The invention discloses a detection method for the logarithmic growth phase of an actinomycete strain producing apramycin, and relates to the technical field of microbial fermentation seed preparation. The detection method comprises the following steps of: S1, strain preservation: preparing a batch of actinomycete seed liquid culture medium and fermentation culture medium, subpackaging the liquidculture medium and the fermentation culture medium into seed shake flasks, setting three parallel experiments, and subpackaging the fermentation culture medium into fermentation shake flasks; and S2,seed culture medium: inoculating the preserved strain into the seed liquid culture medium in the step S1, taking the culture medium which is not inoculated with the strain as a blank control, and carrying out culture under the same condition and synchronizing with seed tank culture. According to the detection method for the logarithmic growth phase of the actinomycete strain producing apramycin, the original measurement technology is combined together and is combined with production experience, so that the defect of a single technology is overcome, the measurement result is more accurate, thetheory and the actual production experience are combined together, the laboratory and the production are tightly combined, and excellent seeds meeting the conditions are provided for production.