6 results about "Apramycin" patented technology

The invention belongs to the technical field of biology, and particularly relates to a construction method and application of an exogenous gene cluster chromosome integration system in streptomyces. The invention reports a new way of integrating an exogenous gene cluster onto a streptomyces chromosome, namely RMCE. The RMCE reduces the influence of other DNA sequences on the stable existence of the gene cluster as much as possible; secondly, the RMCE landing site still has functions after recombination and can be reused; in addition, lox71 and lox66 sites are added on a Dre, Vika and PhiBT1 mediated integration vector for knockout of the resistance gene of the apolamycin, so that the resistance gene can be conveniently used for multiple times. In addition, the recombinase Cre, the recombinase Dre and the recombinase Vika are used for integrating gene clusters in the streptomycete for the first time, the function of the tyrosine recombinase in the streptomycete is expanded, and therefore the tyrosine recombinase has good practical application value.

The invention constructs a biosensor which responds to the apramycin concentration and generates obvious fluorescence signal change, and provides a biosensor skeleton which can be adapted to other antibiotics. Specifically, the invention relates to the expression cassette comprising the apramycin resistance gene sequence, the apramycin resistance gene sequence comprising the expression cassette, a method for preparing the apramycin biosensor, and application of the apramycin biosensor to detection of a bacterial strain for producing apramycin. The method accelerates the step of screening the high-yield apramycin strain, provides a biosensor skeleton for screening other antibiotics, and potentially accelerates the screening of the antibiotic high-yield strain with the corresponding resistance gene.

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

The invention relates to the technical field of biological medicine detection, in particular to a method for detecting apramycin by using a quantum dot fluorescence immunoassay method. A sample and an apramycin monoclonal antibody solution are added into a coated elisa plate for incubation, then a fluorescent probe solution is subjected to incubation reaction, the fluorescence intensity is measured, and finally the apramycin content in the sample is calculated. CdSe / ZnS quantum dots and rabbit anti-mouse IgG are combined to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of apramycin in animal feed, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay method, the fluorescence immunoassay method is more sensitive.

The present invention relates to novel complexes of gallium and apramycin. The complexes according to the invention are useful in the treatment of bacterial infections. The complexes are particularly suitable for administration by inhalation when they are provided in the form of spray-dried particles.

The invention belongs to the technical field of veterinary drugs, discloses a preparation method and application of compound plant volatile oil for preventing and treating bacterial enteritis of meat ducks, and particularly discloses a water-soluble compound plant volatile oil solution. Comprising the following components: cinnamon volatile oil, origanum volatile oil, vitamins, oligosaccharide, a surfactant, a co-emulsifier, a preservative and an antioxidant. The compound plant volatile oil solution disclosed by the invention has a relatively strong in-vitro antibacterial effect, can replace antibiotics such as neomycin sulfate, apramycin and colistin sulfate to effectively prevent and treat enteritis of the meat ducks, has no withdrawal period and no medicine residue, improves the clinical dilute fecal symptom, improves the intestinal health degree and slaughter weight of ducks, ensures the slaughter rate, reduces the feed conversion ratio, and has a good market prospect. The weight of duck gizzards and duck intestines is increased, and the economic benefits of meat duck breeding and slaughtering are improved.