4 results about "Apramycin" patented technology

The invention constructs a biosensor which responds to the apramycin concentration and generates obvious fluorescence signal change, and provides a biosensor skeleton which can be adapted to other antibiotics. Specifically, the invention relates to the expression cassette comprising the apramycin resistance gene sequence, the apramycin resistance gene sequence comprising the expression cassette, a method for preparing the apramycin biosensor, and application of the apramycin biosensor to detection of a bacterial strain for producing apramycin. The method accelerates the step of screening the high-yield apramycin strain, provides a biosensor skeleton for screening other antibiotics, and potentially accelerates the screening of the antibiotic high-yield strain with the corresponding resistance gene.

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

The invention relates to the technical field of biological medicine detection, in particular to a method for detecting apramycin by using a quantum dot fluorescence immunoassay method. A sample and an apramycin monoclonal antibody solution are added into a coated elisa plate for incubation, then a fluorescent probe solution is subjected to incubation reaction, the fluorescence intensity is measured, and finally the apramycin content in the sample is calculated. CdSe / ZnS quantum dots and rabbit anti-mouse IgG are combined to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of apramycin in animal feed, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay method, the fluorescence immunoassay method is more sensitive.

The present invention relates to novel complexes of gallium and apramycin. The complexes according to the invention are useful in the treatment of bacterial infections. The complexes are particularly suitable for administration by inhalation when they are provided in the form of spray-dried particles.