329 results about "Immune profiling" patented technology

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

The invention belongs to the technical field of immunoassay and biosensing detection, and particularly relates to a preparation method and application of an antibiotic electrochemical luminescence sensor based on a double-enhancement strategy. According to the invention, a bifunctional metal organic gel is used as a substrate, a graphite-phase carbon nitride quantum dot is used as a luminous body, and a self-assembly split aptamer walker is combined to construct the electrochemical luminescence sensor for sensitive detection of antibiotics. The sensor has the advantages of extremely high specificity and sensitivity, wide detection range, low detection limit and strong anti-interference performance, and has important scientific significance and application value in detection of antibiotic residues in the environment.

The invention discloses a preparation method of Fe3O4 magnetic nanoparticles rich in carboxyl groups on the surface, which comprises the following steps: modifying Fe3O4 nanoparticles by using polyethylene glycol and silicon dioxide to enhance the dispersity and biocompatibility of the magnetic nanoparticles, modifying the surfaces of PEG-Fe3O4 coated SiO2 nanoparticles by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface, and modifying the magnetic nanoparticles rich in carboxyl groups on the surface by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface. Then octavinyl-POSS and double bonds on the surfaces of the nano particles are subjected to click modification to form an octahedral cage, 4-mercaptobenzoic acid and double bonds are further subjected to click modification, the surfaces of the nano particles are rich in carboxyl, and a polymer brush rich in carboxyl is constructed. Compared with a conventional streptavidin coupling method, a high-density polymer chain provides a large number of carboxyl active binding sites, so that the streptavidin coupling amount is increased, the antibody coupling rate is increased, and a solution is provided for applications such as high-sensitivity biological detection, efficient targeted drug delivery and stable immunoassay.

Provided is an ultrasensitive method for measuring an anti-drug antibody that is simpler and less expensive than conventional methods. Provided is an ultrasensitive method for measuring an analyte using a capture probe and an assist probe and adopting an improved PALSAR method. By using the capture probe and the assist probe and adopting the improved PALSAR method in a double antigen bridging immunoassay, ultrasensitive measurement of an anti-drug antibody can be performed simply and inexpensively.

The present disclosure relates to predicting genomic instability status in biological samples using machine learning techniques with comprehensive genomic and immune profiling (CGIP) data. Particularly, aspects are directed towards performing a genomic instability test on a biological sample. Then, multi-omics data for the subject are obtained by DNA sequencing and RNA sequencing assays, including genomic alteration data for a first set of genes and expression data for a second set of immune genes. The multi-omics data are input into a machine learning model having a tree-based architecture, which is configured to analyze features by traversing paths from root nodes to terminal nodes in each tree based on values generated from the data. The model predicts a genomic instability status, which is then provided via a user interface notification or as part of a testing report.

The invention provides a safrole hapten and application of the safrole hapten in detection of safrole through immunoassay. The structure of the safrole hapten is shown in the specification. The two safrole haptens provided by the invention have spacer arms with proper lengths, and after the safrole haptens are coupled with carrier protein, the haptens can be fully exposed, and recognition of the spacer arms can be avoided as much as possible. The safrole hapten is used for preparing an artificial antigen and an antibody, and the obtained safrole monoclonal antibody is high in titer, strong in specificity and high in affinity. An immunoassay method constructed on the basis of the safrole artificial antigen and antibody has high specificity and sensitivity, the lowest detection limit LOD is 0.09 ng / mL, the half inhibitory concentration IC50 is 2.05 ng / mL, the quantitative detection range is 0.28-14.89 ng / mL, no cross reaction exists on safrole analogues, and rapid qualitative and quantitative detection can be conducted on safrole in a sample.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention provides an analgin hapten, a preparation method thereof, an artificial antigen, an antibody and application. The structures of the analgin haptens are as shown in a formula (I) or a formula (II), the two analgin haptens provided by the invention have spacer arms with proper lengths, and after the two analgin haptens are coupled with protein, the haptens can be fully exposed to an immune system, the spacer arms can be prevented from being recognized as much as possible, and a better antibody is generated to recognize analgin. The analgin hapten is used for preparing artificial antigen and antibody, the obtained analgin monoclonal antibody has high titer and strong specificity, an immunoassay method constructed based on the analgin monoclonal antibody has high specificity and sensitivity, analgin in a sample can be rapidly, qualitatively and quantitatively detected, the operation is simple and convenient, and the detection result is accurate and reliable.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

The utility model relates to the technical field of detection equipment, in particular to a fluorescence immunoassay analyzer. Comprising a machine body, a detection window is arranged on the front side of the machine body, a multi-channel reaction disc is arranged on one side of the detection window, a plurality of incubation grooves are formed in the front side of the multi-channel reaction disc, the multi-channel reaction disc comprises a plurality of independently arranged timing modules, buzzers and indicator lamps, and the buzzers and the indicator lamps are electrically connected with the timing modules. When the reaction time of the test card on a certain channel in the multi-channel reaction disc is over, the buzzer and the indicator light can give an alarm; the device can immediately detect a plurality of temperature-controlled test cards in sequence, and does not need to wait for the completion of the temperature control of the next test card, so that a large amount of time can be saved.

The present invention provides a quantitative measurement method using competitive immunochromatography, which has not been previously reported. The quantitative measurement method comprises: (1) creating a calibration curve based on the signal intensity of a test line according to the concentration of a standard material by using multiple strips, and calculating the concentration of a target analyte by using the calibration curve; (2) when the concentration of the target analyte increases to a significant level on the calibration curve of the standard material, a labeled signal of a detection ligand on a control line remains unchanged in a case where a detection ligand of a target analyte-detection ligand conjugate binds to and saturates a capture ligand on the control line, making it impossible to estimate the concentration beyond that level; however, by designing a binding site for the capture ligand on the control line to be the target analyte of the target analyte-detection ligand conjugate, a high concentration of the target analyte breaks down the triple conjugate of detection ligand-target analyte-capture ligand, leading to dissociation of the labeled detection ligand and thereby weakening the signal (hook effect); and (3) comparing the signal intensity patterns of the test line and the control line according to the calibration curve of the standard material with the signal intensity pattern of a sample target analyte, and determining how far the target analyte is from the saturation point of the detection ligand. This method is characterized in that, in cases where the target analyte is present in an excessively large amount and exceeds the maximum value of a measurement range (dynamic range) of the calibration curve, the calibration curve using multiple strips and the configurations (test line and control line) of an immunochromatographic device are designed differently to distinguish such cases.

The invention discloses a multifunctional glass microbubble, a high-flux rapid immunoassay system and application thereof, and the multifunctional glass microbubble is prepared by the following steps: S1, adding a bovine serum albumin solution into hollow glass microbubbles to obtain a bovine serum albumin coating, centrifuging, removing a lower-layer solution, cleaning and drying to obtain a hollow glass microbubble solution; the antifouling hollow glass microbubbles are obtained; s2, mixing the antifouling hollow glass microbubbles with tetrazine-active ester, centrifuging, removing a lower solution, washing and drying to obtain the antifouling hollow glass microbubbles with the biological orthogonal function. The anti-fouling hollow glass microbubble has the beneficial effects that the anti-fouling hollow glass microbubble with a biological orthogonal function, the self-suspension performance, the anti-fouling function and the bovine serum albumin rich in tyrosine residues not only can realize high-flux array detection in a 96-well plate, but also can realize the effects of anti-fouling and signal amplification, and can be applied to the field of biosensor detection. Therefore, the defects of low flux, low sensitivity, long detection time and the like of a traditional detection method are overcome.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

This application relates to a detection reagent, kit, and method that simultaneously improves sensitivity and broadens the detection range in competitive immunoassay. The reagent includes a detection antibody bound to luminescent microspheres that specifically binds to analyte molecules in the sample, and two sets of labeled antigens containing the same competing antigen and labeled molecules of different molecular weights bound to it. The competing antigen competes with the analyte molecule for binding to the detection antibody, and the affinity of the competing antigen for binding to the detection antibody is higher than that of the analyte molecule for binding to the detection antibody. The technical solution of this application, by using two sets of labeled antigens with different affinities, ensures that the labeled antigens that play a dominant role in samples with varying concentrations of analyte molecules differ. This is suitable for competitive immunoassay of small molecule antigens or haptens in both low-concentration and high-concentration samples, and can broaden the detection range while ensuring high functional sensitivity.

The present application relates to the field of immunoassay technology, and more particularly to a preparation method of Netrin-1 antibody coated magnetic microparticles, the magnetic microparticles prepared by the method and application thereof. The present application provides a preparation method of Netrin-1 antibody coated magnetic microparticles, the magnetic microparticles prepared by the method and application thereof. The method steps of the present application support each other, interact with each other, and together achieve a good magnetic bead coating effect. The prepared magnetic beads have high specificity, high sensitivity, good stability and higher accuracy. The kit of the present application realizes low background and high signal of the immune response, has good specificity, and makes the antigen and antibody have high detection sensitivity. The kit realizes automatic determination. The kit of the present application can be used with a full-automatic chemiluminescence instrument, reduces the influence of human operation on the test results, makes the detection results more reliable, more accurate, faster and more repeatable, and therefore has a good clinical application prospect.

This application provides an immunoassay analyzer, a magnetic separation device, and a method for cleaning the aspiration needle, belonging to the technical field of immunoassay equipment. The immunoassay analyzer includes a magnetic separation device, which includes a magnetic separation disk, a liquid injection device, an aspiration needle, and a controller. It also includes a separation disk heating module, or a separation disk heating module and a cleaning agent heating module. The magnetic separation device has a first mode and a second mode. In the first mode, it can clean the magnetic particles in the reaction cup. In the second mode, the controller can control the heating module to heat the aspiration needle cleaning agent to a first temperature. The aspiration needle cleaning agent has high activity and cleaning ability at the first temperature, and can efficiently clean the deposits attached to the inner wall of the aspiration needle.

This invention relates to a hybridoma cell line secreting a monoclonal antibody against pendimethalin and its application, belonging to the field of immunoassay. The hybridoma cell line secreting the pendimethalin monoclonal antibody of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46744. The pendimethalin monoclonal antibody secreted by this hybridoma cell line is used for the analysis and detection of pendimethalin residues in food safety testing. The pendimethalin monoclonal antibody cell line obtained by this invention can be used for immunoassay detection, exhibiting good detection sensitivity and specificity (IC50) for pendimethalin. 50 The value was 1.87 ng / mL.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

This invention belongs to the field of immunoassay and biosensing technology, and discloses a method for preparing a ratiometric ECL biosensor based on Eu / Tb-Lu MOGs. Using Eu / Tb-Lu MOGs as the luminescent material and H3-tctpy and luminol as ligands, a single-luminescent material with dual ECL emission is synthesized. Lanthanide ions provide the cathode ECL emission, enhanced by the antenna effect and Tb→Eu energy transfer; the anode emits light from luminol using dissolved oxygen in water, requiring only the addition of K2S2O8 as a single co-reactant, resulting in fewer side reactions and a stable signal. The dual-potential ratiometric sensing utilizes the dual ECL signal for self-calibration, improving anti-interference and reliability; the single-luminescent dual-potential system achieves synchronous anode / cathode luminescence, simplifying the system and improving detection accuracy. By detecting changes in the ECL signal of different concentrations of analytes, the detection of microRNA-126 is achieved.

The application discloses a kind of diflufenican hapten, antigen, antibody and its preparation method and application.The application prepares diflufenican hapten as shown in formula (I) and corresponding diflufenican antigen, and specific antibody for detecting diflufenican is prepared by diflufenican antigen, and further constructs the immune analysis method for directly detecting diflufenican pesticide residue, which is particularly suitable for on-site rapid detection, and can meet the needs of regulatory authorities and detection agencies formula (I).

The invention relates to a liquid injection module, an immunoassay device and a liquid injection method.The immunoassay device comprises an incubation module and the liquid injection module, the incubation module comprises a reaction disc cover, the liquid injection module comprises a support, a liquid injection assembly, a heating assembly and a driving assembly, the driving assembly can drive the support to slide on the reaction disc cover, and the liquid injection assembly is installed on the support; the liquid injection assembly comprises a liquid injection head and a liquid bottle assembly, a to-be-injected object is stored in the liquid bottle assembly, the heating assembly comprises a heating pipeline and a heating part, the heating part can heat the heating pipeline, one end of the heating pipeline can be communicated with the liquid bottle assembly, and the other end of the heating pipeline is communicated with the liquid injection head. After the heating assembly heats the to-be-injected object, the to-be-injected object is injected into the reaction cup through the liquid injection assembly, the to-be-injected object does not need to be heated in the reaction cup, the incubation time is shortened, and the incubation effect is guaranteed. The liquid injection method is achieved through the liquid injection module, liquid injection, cleaning and to-be-injected object replacement can be achieved, incubation in the immunoassay process is facilitated, and the immunoassay efficiency is improved.

The application discloses a kind of filling assembly and control method, magnetic separation cleaning device.Adding component includes cup rotating member and injection needle, cup rotating member is used to grab reaction cup and drive it to rotate, and its central axis is provided with injection channel;Injection needle is coaxially arranged with cup rotating member and can be from injection channel into reaction cup.In control timing, cup rotating member first drives reaction cup to rotate, and after it is in rotating state, injection needle then adds cleaning fluid or substrate into reaction cup.The design will overlap the timing of filling and mixing steps, effectively reduce the filling splash and magnetic bead loss, improve the purification efficiency, and can complete efficient processing in the short residence time of magnetic separation carousel, especially suitable for high-throughput chemiluminescence immunoassay equipment, while ensuring detection accuracy significantly improve detection throughput.

The utility model relates to a reagent disc applied to an immunity analyzer. The reagent disc comprises a reagent disc, a fixed cover fixedly mounted at the top end of the reagent disc and a movable cover detachably mounted, a reagent pot is detachably mounted in the reagent disc, and the outer wall of the reagent pot abuts against the inner wall of the reagent disc; a reagent tray is rotatably installed in the reagent pot in a limited mode, a plurality of reagent boxes are sequentially rotatably installed on the reagent tray in the circumferential direction in a limited mode, pinions are detachably installed on the reagent boxes, fixed gears are detachably installed in the reagent pot, the fixed gears and the reagent tray are coaxially arranged, and the fixed gears are meshed with the pinions. A rotating mechanism is mounted on the reagent tray and is in transmission fit with the reagent tray; a refrigeration mechanism for cooling the interior of the reagent pot is mounted on the reagent pot; according to the chemiluminescence immunoassay kit, reagents for chemiluminescence immunoassay can be uniformly mixed and refrigerated, so that the accuracy of subsequent detection results is improved.

The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

The present invention concerns a diagnostic device and method for detecting thiamine and Vitamin A deficiencies using salivary biomarkers. The device utilizes a lateral flow assay to detect secretory Immunoglobulin A (sIgA) and amylase in saliva, providing a non-invasive, easy-to-use, and reliable method for assessing nutritional deficiencies. This invention is particularly useful for monitoring health status in populations with limited access to medical facilities.

The application relates to a preparation method of a Pt single-atom nanoscale enzyme and application of the Pt single-atom nanoscale enzyme in detection of cardiac troponin I, and the preparation method comprises the following steps: 1) ultrasonic dispersion of cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized, grinding the solid mixture into a powder; 2) performing Joule heat treatment on the powder in a heating device under an air atmosphere, and performing washing and vacuum drying on the reactants to obtain the Pt single-atom nanoscale enzyme. In the one-step chemical reaction process, Se doping is introduced to construct oxygen vacancies, rapid heating and structure shaping are realized by combining the Joule heat treatment, a Pt single-atom catalyst loaded with Se-doped Co3O4 is obtained, the oxygen vacancies and the Se doping sites are used as anchor sites in cooperation, the Pt single atoms are effectively fixed, and the loading capacity and the dispersity of the Pt single atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The utility model provides a fluorescence immunoassay analyzer, which is used for detecting a reagent card and comprises an analyzer body and a positioning block, the analyzer body is provided with an inlet / outlet and an accommodating groove communicated with the inlet / outlet; the positioning block is arranged in the accommodating groove in a penetrating manner and is detachably connected to the analyzer body; the positioning block is provided with a clamping groove and an elastic buckle, and the reagent card can move along the clamping groove and is clamped with the elastic buckle. According to the utility model, the positioning block provided with the elastic buckle is detachably connected to the analyzer body, so that when the elastic buckle is subjected to structural fatigue or damage due to frequent insertion and extraction of the reagent card, the analyzer body does not need to be disassembled, and only the positioning block needs to be taken out of the accommodating groove for replacement, thereby simplifying the maintenance process, shortening the maintenance time and improving the maintenance efficiency. The risk of secondary damage to precise detection elements in the analyzer in the disassembling process is also avoided, the maintenance efficiency of equipment is improved, and the service life of the equipment is prolonged.

The present invention belongs to the field of biochemical technology, and specifically relates to an artificial hapten and an artificial antigen for benzhexyphenidyl, as well as a preparation method and application thereof. The molecular structural formula of the artificial hapten for benzhexyphenidyl is shown in Formula (I), and the molecular structural formula of the artificial antigen for benzhexyphenidyl is shown in Formula (II). The artificial hapten for benzhexyphenidyl can be used in the field of preparing anti-benzhexyphenidyl antibodies. The artificial hapten for benzhexyphenidyl of the present invention retains the characteristic structure of benzhexyphenidyl to the greatest extent, and has an active group that can be coupled with a carrier protein, and can serve as an antigenic determinant. The artificial hapten for benzhexyphenidyl obtained by further preparation can be used to immunize and obtain anti-benzhexyphenidyl antibodies with high affinity, high sensitivity, and strong specificity. The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:256,000, and can be used for rapid and accurate immunodetection and immunoanalysis of benzhexyphenidyl.