461 results about "Immune profiling" patented technology

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention discloses a sample concentration detection method, a standard curve group establishment method, a matrix specificity standard curve optimization method, a standard curve group establishment device and an immunity analyzer, and belongs to the field of diagnostic analysis. Acquiring matrix information of a to-be-detected sample; selecting a matrix specificity standard curve corresponding to the matrix of the sample to be detected; and detecting the sample and calculating the concentration of the analyte in the sample to be detected by using a matrix specificity standard curve selected from a plurality of standard curve groups of which the specificity corresponds to different matrixes. The corresponding substrate specificity standard curves are configured for various types of substrates in advance, and when a detection task is carried out, the corresponding substrate specificity standard curves can be selected and called, so that the detection is more targeted, the substrate effect caused by using a general standard curve can be avoided, the substrate effect can be reduced, the implementation operation is simpler, and the detection efficiency is improved. And the working time of detection personnel can be remarkably saved, and the detection cost is effectively reduced.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

A fully automated single‑molecule immunoassay analyzer, comprising a sample adding module (1), a reagent module (4), an incubation temperature control module (7), and an optical detection module. The incubation temperature control module (7) comprises: a turntable (85) provided with multiple circumferentially arranged bearing positions (86) for reaction vessels (57); a motor (81) and a first rotating shaft (82), the turntable (85) being fixed on the first rotating shaft (82), and the motor (81) driving the first rotating shaft (82) to rotate; a heating component and a temperature sensor disposed on the turntable (85); an enclosure (84) and an upper cover (87), the enclosure (84) surrounding the turntable (85), and the upper cover (87) being disposed at an upper opening of the enclosure (84); and a mixing mechanism (5) disposed below the turntable (85), wherein when the turntable (85) rotates and a reaction vessel (57) at any of the bearing positions (86) is above the mixing mechanism (5), the mixing mechanism (5) lifts the reaction vessel (57) and rotates.

The invention belongs to the technical field of immunoassay and biosensing, and provides a novel construction mode of a heterojunction dual-photoelectrode photoelectric sensing strategy and a preparation method of an in-situ heterojunction MIO photocathode. The invention relates to a preparation method of a heterojunction dual-photoelectrode type photoelectrochemical sensor based on multi-ball cascade DNA reaction amplification. Specifically, the design is composed of an In2O3 / CdS photoanode and an in-situ formed MIL-68 / In2O3 (MIO) photocathode. The heterojunction dual-photoelectrode sensing strategy effectively prolongs an electron-hole transmission path, enhances photo-generated charge separation, and realizes' super-open 'PEC signal output, thereby providing a steady baseline for signal conversion. Meanwhile, a multi-sphere cascade DNA reaction is combined to output a three-dimensional DNA net structure to provide a large number of sites for embedding of a PEC quenching probe doxorubicin-ferrocene carboxylic acid (DOX-FcCOOH), obvious PEC signal quenching is realized by utilizing the efficient competitive electron transfer capability of the three-dimensional DNA net structure, and finally, a signal conversion system with'opening-super opening-closing 'multistage response characteristics is constructed. The detection of the nucleic acid marker Pax-5a gene of the B cell acute lymphatic leukemia is realized by detecting target objects with different concentrations.

The invention belongs to the technical field of immunoassay and biosensing detection, and particularly relates to a preparation method and application of an antibiotic electrochemical luminescence sensor based on a double-enhancement strategy. According to the invention, a bifunctional metal organic gel is used as a substrate, a graphite-phase carbon nitride quantum dot is used as a luminous body, and a self-assembly split aptamer walker is combined to construct the electrochemical luminescence sensor for sensitive detection of antibiotics. The sensor has the advantages of extremely high specificity and sensitivity, wide detection range, low detection limit and strong anti-interference performance, and has important scientific significance and application value in detection of antibiotic residues in the environment.

The invention relates to the technical field of immunoassay, in particular to a full-automatic single-molecule fluorescence immunoassay analyzer which comprises an analyzer body and a sample adding bin and further comprises a plurality of transfer pipes located in the sample adding bin. The knocking rods are located in the sample adding bin, the knocking rods are used for knocking and vibrating the bottom of the transfer pipe, when the full-automatic single-molecule fluorescence immunoassay analyzer is used, the existence of bubbles can interfere with reading of fluorescence signals, so that an analysis result is inaccurate, the bubbles are discharged through knocking and vibrating, and the accuracy of the analysis result is improved. According to the method, accurate reading of fluorescence signals can be ensured, so that the analysis accuracy is improved, the fluorescence signal intensity is optimized, bubbles can shield part of the fluorescence signals, the signal intensity is weakened, after the bubbles are discharged, the fluorescence signals can be more fully detected, the signal intensity is optimized, and the result is clearer.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention belongs to the technical field of treatment equipment, and relates to a reagent treatment device of a single molecule immunity analyzer, which comprises a treatment box, the upper part of the treatment box is open, and a reagent disc is arranged in the opening; the device comprises a fixed seat, a rotating shaft, a moving rod, a rotating assembly, a lifting assembly, an oscillating assembly and a clutch assembly. When the movable rod is driven to move upwards, the movable rod is connected with the fixed seat to drive the fixed seat to rotate so as to perform centrifugal treatment on a reagent, and when the movable rod is driven to move downwards, the movable rod is connected with the oscillation assembly to drive the fixed seat to perform circumferential oscillation so as to perform vortex treatment on the reagent; the time interval between each centrifugation and vortex can be kept consistent, the sedimentation uniformity is improved, the time required by the whole process can be shortened, sedimentation particles are prevented from caking, uniform mixing during vortex is more thorough, and the accuracy and repeatability of detection are guaranteed.

The invention relates to the technical field of fluorescence immunoassay, and discloses a quantum resonance fluorescence immunoassay medical detection method, which comprises the following steps: 1, carrying out fixation and permeation treatment on cells, respectively incubating the cells by using labeled different quantum dot-antibody compounds, specifically binding antibodies with corresponding target proteins in the cells, and detecting the target proteins in the cells; a labeled cell sample is obtained; 2, under a confocal microscope, laser with a specific wavelength is adopted to excite the marked cell sample, and fluorescence lifetime image data of different quantum dot emission wavelengths are collected; according to the method, the fluorescence lifetime image data of different quantum dot emission wavelengths are acquired through the confocal microscope, the position and content information of different target proteins can be clearly distinguished, simultaneous analysis and accurate positioning of multiple target proteins are realized, and an efficient means is provided for multi-protein research in a complex biological system.

The invention discloses a preparation method of Fe3O4 magnetic nanoparticles rich in carboxyl groups on the surface, which comprises the following steps: modifying Fe3O4 nanoparticles by using polyethylene glycol and silicon dioxide to enhance the dispersity and biocompatibility of the magnetic nanoparticles, modifying the surfaces of PEG-Fe3O4 coated SiO2 nanoparticles by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface, and modifying the magnetic nanoparticles rich in carboxyl groups on the surface by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface. Then octavinyl-POSS and double bonds on the surfaces of the nano particles are subjected to click modification to form an octahedral cage, 4-mercaptobenzoic acid and double bonds are further subjected to click modification, the surfaces of the nano particles are rich in carboxyl, and a polymer brush rich in carboxyl is constructed. Compared with a conventional streptavidin coupling method, a high-density polymer chain provides a large number of carboxyl active binding sites, so that the streptavidin coupling amount is increased, the antibody coupling rate is increased, and a solution is provided for applications such as high-sensitivity biological detection, efficient targeted drug delivery and stable immunoassay.

The invention belongs to the technical field of immunoassay and biosensing, and provides a preparation method of a photoelectrochemical sensor based on CuO-induced photocurrent polarity reversal. The invention provides a preparation method of the photoelectrochemical sensor based on CuO-induced photocurrent polarity reversal. Specifically, hollow polyhedral flower-shaped CuO with a unique inner cavity structure is adopted as a polarity induction factor, so that multiple light refraction and scattering effects are remarkably enhanced, and the light capture efficiency is improved. Besides, when CuO is introduced to the surface of the In2O3 / ZnCdS electrode as a photocurrent polarity reversal factor, due to the energy band structure difference between CuO and In2O3 / ZnCdS, the migration path of a photon-generated carrier is changed, and the photocurrent polarity is reversed from an anode to a cathode photocurrent, so that the detection specificity of the gastrin-releasing peptide is improved. The photocurrent polarity reversal strategy effectively eliminates potential false positive signal interference, and the accuracy and sensitivity of detection are remarkably improved. According to different photoelectrochemical signal intensities of to-be-detected substances with different concentrations, the gastrin-releasing peptide is detected.

The invention relates to the technical field of antibody detection methods, in particular to a diagnosis method and a detection system based on autoimmune disease antibody detection, and the method comprises the following steps: S1, an analysis module obtains electrochemistry related information and light reflection related information through electrochemical analysis and light reflection analysis; related information of electrochemistry and related information of light reflection are transmitted to the control module; s2, the control module obtains an antibody concentration index according to the electrochemistry related information and the light reflection related information, and transmits the antibody concentration index to a diagnosis module; s3, the diagnosis module obtains strong or weak immunoreaction information according to the antibody concentration index, and transmits the strong or weak immunoreaction information to the communication module; and S4, the communication module transmits strong or weak immunoreaction information to the user side. Immunoassay is combined with electrochemical analysis and light reflection analysis for detection, and the accuracy and comprehensiveness of detection can be improved by analyzing in different directions and combining results.

Provided is an ultrasensitive method for measuring an anti-drug antibody that is simpler and less expensive than conventional methods. Provided is an ultrasensitive method for measuring an analyte using a capture probe and an assist probe and adopting an improved PALSAR method. By using the capture probe and the assist probe and adopting the improved PALSAR method in a double antigen bridging immunoassay, ultrasensitive measurement of an anti-drug antibody can be performed simply and inexpensively.

The present disclosure relates to predicting genomic instability status in biological samples using machine learning techniques with comprehensive genomic and immune profiling (CGIP) data. Particularly, aspects are directed towards performing a genomic instability test on a biological sample. Then, multi-omics data for the subject are obtained by DNA sequencing and RNA sequencing assays, including genomic alteration data for a first set of genes and expression data for a second set of immune genes. The multi-omics data are input into a machine learning model having a tree-based architecture, which is configured to analyze features by traversing paths from root nodes to terminal nodes in each tree based on values generated from the data. The model predicts a genomic instability status, which is then provided via a user interface notification or as part of a testing report.

The invention provides a safrole hapten and application of the safrole hapten in detection of safrole through immunoassay. The structure of the safrole hapten is shown in the specification. The two safrole haptens provided by the invention have spacer arms with proper lengths, and after the safrole haptens are coupled with carrier protein, the haptens can be fully exposed, and recognition of the spacer arms can be avoided as much as possible. The safrole hapten is used for preparing an artificial antigen and an antibody, and the obtained safrole monoclonal antibody is high in titer, strong in specificity and high in affinity. An immunoassay method constructed on the basis of the safrole artificial antigen and antibody has high specificity and sensitivity, the lowest detection limit LOD is 0.09 ng / mL, the half inhibitory concentration IC50 is 2.05 ng / mL, the quantitative detection range is 0.28-14.89 ng / mL, no cross reaction exists on safrole analogues, and rapid qualitative and quantitative detection can be conducted on safrole in a sample.

The invention belongs to the technical field of immunoassay rapid detection, and relates to a method for screening residues of traditional Chinese medicinal material aflatoxin B1 based on multicolor evolution fluorescence immunochromatography, which comprises the following steps: coupling red quantum dot fluorescent microspheres with an AFB1 antibody to prepare a detection probe; coupling or mixing the green quantum dot fluorescent microspheres with an AFB1 antigen to prepare an internal standard probe; respectively coating an internal standard probe and goat anti-mouse IgG on an NC membrane to complete preparation of a T line and a C line; preparing a test solution containing a detection probe, dripping the test solution on a test paper sample pad, and observing fluorescence colors of a T line and a C line by ultraviolet; and judging whether the residual quantity of the traditional Chinese medicine AFB1 exceeds the limit specification or not according to the T-line fluorescence color. By introducing the internal standard probe, a ratio fluorescence immunoassay system is constructed, so that the sensitivity and the accuracy of an analysis result can be improved, and the interference of a sample matrix is reduced; the used detection test paper is small in matrix influence and wide in application range, and can be used for rapid limited screening of AFB1 residues in traditional Chinese medicinal materials.

The invention provides multi-channel multi-joint inspection POCT full-automatic chemiluminescence equipment. The equipment comprises a rack; the sampling gun head accommodating mechanism, the test tube accommodating mechanism and the HGB detection mechanism are arranged on the rack in a longitudinal reciprocating motion manner, and a treating fluid container is arranged on the sampling gun head accommodating mechanism; the sampling mechanism is arranged on the rack in a transverse reciprocating motion manner; the reaction bin mechanism can be longitudinally arranged on the rack in a reciprocating manner and comprises a reaction bin body used for loading multiple joint inspection reagent strips, and the reaction bin body is provided with pressing elastic pieces used for pressing the multiple joint inspection reagent strips; the clamping and locking mechanism is located below the reaction bin mechanism, the reaction bin mechanism further comprises a driving device and a synchronous conveying belt connected with the driving device and a reaction bin body, and the clamping and locking mechanism is used for clamping, locking or loosening and unlocking the synchronous conveying belt so as to lock or unlock the reaction bin body. According to the present invention, the device has the immunoassay function, the HGB detection can be achieved, the multi-joint detection reagent strip does not shake or tilt, and the reaction bin body can be locked.

The invention relates to the technical field of machinery, and discloses a high-speed full-automatic chemiluminescence immunoassay analyzer and a control method thereof.The high-speed full-automatic chemiluminescence immunoassay analyzer comprises an immunoassay analyzer body, a dilution module is arranged in the immunoassay analyzer body, and a cleaning module is arranged in the immunoassay analyzer body; the immunity analyzer comprises a cup arranging device module, a sample arm module is arranged on the back face of the cup arranging device module, the right side of the sample arm module and a dilution module are arranged, the dilution module comprises a dilution needle, a motor, a transfer sliding block base, a dilution disc, a transfer disc, a pipetting needle, a uniform mixing mechanism A and a uniform mixing mechanism B, and the sample arm module comprises a sample needle. The transfer disc is provided with 36 cup positions in total, and three cup positions can be rotated at a time. According to the high-speed full-automatic chemiluminescence immunoassay analyzer and the control method thereof, the information of the placed reagent disc can be automatically diluted and detected, manual operation is not needed, the full-automatic detection capability is improved, and the positioning and monitoring functions of the reagent cup are enhanced.

The invention provides an immunoassay method based on instant synthesis of a nano-enzyme of a bimetallic CuCo Prussian blue analogue (CuCo PBA), and belongs to the field of chemical and biological sensing. According to the method, a sandwich type immune mode is formed by a glucose oxidase labeled antibody, a target object and an immobilized antibody, so that the concentration of the target object is in direct proportion to the amount of glucose oxidase. Hydrogen peroxide generated by catalyzing glucose by glucose oxidase catalyzes 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to oxidize and develop color under the assistance of CuCo PBA nano-enzyme (Cu < 2 + > and K3 [Co (CN) 6]) which is generated instantly (Cu < 2 + > and K3 [Co (CN) 6] are added instantly). And the color change of the detection liquid is recorded and is used for visual detection and quantitative detection by a microplate reader. The problems of advanced synthesis and storage of nano materials are avoided, the cost is low, the colorimetric detection method is simple in operation process, rapid in color development and high in sensitivity, and a novel method which is convenient and stable in performance is provided for clinical immunological detection.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention provides an analgin hapten, a preparation method thereof, an artificial antigen, an antibody and application. The structures of the analgin haptens are as shown in a formula (I) or a formula (II), the two analgin haptens provided by the invention have spacer arms with proper lengths, and after the two analgin haptens are coupled with protein, the haptens can be fully exposed to an immune system, the spacer arms can be prevented from being recognized as much as possible, and a better antibody is generated to recognize analgin. The analgin hapten is used for preparing artificial antigen and antibody, the obtained analgin monoclonal antibody has high titer and strong specificity, an immunoassay method constructed based on the analgin monoclonal antibody has high specificity and sensitivity, analgin in a sample can be rapidly, qualitatively and quantitatively detected, the operation is simple and convenient, and the detection result is accurate and reliable.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

The invention relates to the technical field of chemiluminescence immunoassay, and discloses a horse radish peroxidase-labeled secondary antibody compound and a preparation method and application thereof, the horse radish peroxidase-labeled secondary antibody compound comprises a chitosan compound skeleton, and horse radish peroxidase and a secondary antibody carried on the chitosan compound skeleton; the chitosan compound skeleton is a macromolecular skeleton compounded by a chitosan spherical cross-linking body and dopamine. Through optimization and improvement of a polymer skeleton, the problems of large steric hindrance, low sensitivity and non-specific staining of a traditional polymer can be solved, and further improvement of the sensitivity of a DAB staining method for immunoassay detection is facilitated; the adopted chitosan compound skeleton has abundant amino groups, can bear more labeled enzymes and secondary antibodies, reduces steric hindrance, increases the penetrating power to a cell nuclear membrane, and improves the detection sensitivity.

The utility model discloses a cleaning disc of a full-automatic single-molecule immunoassay device, which comprises a cleaning disc barrel, a turntable mechanism arranged in the cleaning disc barrel and a needle body lifting mechanism arranged above the cleaning disc barrel, a reaction cup is placed on a turntable of the turntable mechanism, and the needle body lifting mechanism is arranged above the cleaning disc barrel. The rotating disc can drive a reaction cup to rotate, a liquid suction needle is arranged on the needle body lifting mechanism, a liquid injection needle is fixedly arranged at the top of a cleaning disc barrel, a cleaning barrel is arranged at the top of the cleaning disc barrel and located below the needle body lifting mechanism, a water receiving barrel is further arranged on the rotating disc, and a water outlet is formed in the water receiving barrel. A drainage cylinder is arranged in the cleaning disc cylinder and located below the rotary disc. And the cleaning water after the liquid injection needle is cleaned and the cleaning water after the liquid suction needle is inserted into the cleaning barrel for cleaning are discharged out of the cleaning disc through the water receiving barrel and the water discharging barrel. When the liquid injection needle and the liquid suction needle are cleaned, the cleaning structure and steps of the liquid injection needle and the liquid suction needle are simplified.

The utility model relates to the technical field of detection equipment, in particular to a fluorescence immunoassay analyzer. Comprising a machine body, a detection window is arranged on the front side of the machine body, a multi-channel reaction disc is arranged on one side of the detection window, a plurality of incubation grooves are formed in the front side of the multi-channel reaction disc, the multi-channel reaction disc comprises a plurality of independently arranged timing modules, buzzers and indicator lamps, and the buzzers and the indicator lamps are electrically connected with the timing modules. When the reaction time of the test card on a certain channel in the multi-channel reaction disc is over, the buzzer and the indicator light can give an alarm; the device can immediately detect a plurality of temperature-controlled test cards in sequence, and does not need to wait for the completion of the temperature control of the next test card, so that a large amount of time can be saved.

The present invention provides a quantitative measurement method using competitive immunochromatography, which has not been previously reported. The quantitative measurement method comprises: (1) creating a calibration curve based on the signal intensity of a test line according to the concentration of a standard material by using multiple strips, and calculating the concentration of a target analyte by using the calibration curve; (2) when the concentration of the target analyte increases to a significant level on the calibration curve of the standard material, a labeled signal of a detection ligand on a control line remains unchanged in a case where a detection ligand of a target analyte-detection ligand conjugate binds to and saturates a capture ligand on the control line, making it impossible to estimate the concentration beyond that level; however, by designing a binding site for the capture ligand on the control line to be the target analyte of the target analyte-detection ligand conjugate, a high concentration of the target analyte breaks down the triple conjugate of detection ligand-target analyte-capture ligand, leading to dissociation of the labeled detection ligand and thereby weakening the signal (hook effect); and (3) comparing the signal intensity patterns of the test line and the control line according to the calibration curve of the standard material with the signal intensity pattern of a sample target analyte, and determining how far the target analyte is from the saturation point of the detection ligand. This method is characterized in that, in cases where the target analyte is present in an excessively large amount and exceeds the maximum value of a measurement range (dynamic range) of the calibration curve, the calibration curve using multiple strips and the configurations (test line and control line) of an immunochromatographic device are designed differently to distinguish such cases.

The invention discloses a multifunctional glass microbubble, a high-flux rapid immunoassay system and application thereof, and the multifunctional glass microbubble is prepared by the following steps: S1, adding a bovine serum albumin solution into hollow glass microbubbles to obtain a bovine serum albumin coating, centrifuging, removing a lower-layer solution, cleaning and drying to obtain a hollow glass microbubble solution; the antifouling hollow glass microbubbles are obtained; s2, mixing the antifouling hollow glass microbubbles with tetrazine-active ester, centrifuging, removing a lower solution, washing and drying to obtain the antifouling hollow glass microbubbles with the biological orthogonal function. The anti-fouling hollow glass microbubble has the beneficial effects that the anti-fouling hollow glass microbubble with a biological orthogonal function, the self-suspension performance, the anti-fouling function and the bovine serum albumin rich in tyrosine residues not only can realize high-flux array detection in a 96-well plate, but also can realize the effects of anti-fouling and signal amplification, and can be applied to the field of biosensor detection. Therefore, the defects of low flux, low sensitivity, long detection time and the like of a traditional detection method are overcome.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a spectral flow cytometer with a least three excitation lasers and thirty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.