650 results about "Immune profiling" patented technology

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention provides a targeted giant salamander iridovirus nano antibody as well as a preparation method and application thereof. The invention discloses a giant salamander iridovirus targeting nano antibody, and aims to provide a giant salamander iridovirus targeting nano antibody which is low in expression cost, low in difficulty and stable in source, can be effectively combined with giant salamander iridovirus, and specifically realizes immunoassay of the giant salamander iridovirus. The amino acid sequences of the nano antibody are shown as SEQ ID NO.1-6, and the nano antibody belongs to the technical field of biological science.

The invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, measuring a signal value generated after the analyte calibrator with each concentration is captured on a detection pad of the immunoassay test strip at each segmented temperature point by using an optical analyzer; drawing a corresponding equation by taking the T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, according to the selected n segmented temperature points, carrying out temperature segmentation, and in different temperature segments, carrying out calculation by adopting different formulas. The analyte detection method subjected to temperature correction can be used for more accurately detecting myocardial markers, upper respiratory virus infection, metabolic hormones, digestive tract series markers, allergens and the like on the basis of a time-resolved fluorescence immunoassay test.

The invention discloses a sample concentration detection method, a standard curve group establishment method, a matrix specificity standard curve optimization method, a standard curve group establishment device and an immunity analyzer, and belongs to the field of diagnostic analysis. Acquiring matrix information of a to-be-detected sample; selecting a matrix specificity standard curve corresponding to the matrix of the sample to be detected; and detecting the sample and calculating the concentration of the analyte in the sample to be detected by using a matrix specificity standard curve selected from a plurality of standard curve groups of which the specificity corresponds to different matrixes. The corresponding substrate specificity standard curves are configured for various types of substrates in advance, and when a detection task is carried out, the corresponding substrate specificity standard curves can be selected and called, so that the detection is more targeted, the substrate effect caused by using a general standard curve can be avoided, the substrate effect can be reduced, the implementation operation is simpler, and the detection efficiency is improved. And the working time of detection personnel can be remarkably saved, and the detection cost is effectively reduced.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

A fully automated single‑molecule immunoassay analyzer, comprising a sample adding module (1), a reagent module (4), an incubation temperature control module (7), and an optical detection module. The incubation temperature control module (7) comprises: a turntable (85) provided with multiple circumferentially arranged bearing positions (86) for reaction vessels (57); a motor (81) and a first rotating shaft (82), the turntable (85) being fixed on the first rotating shaft (82), and the motor (81) driving the first rotating shaft (82) to rotate; a heating component and a temperature sensor disposed on the turntable (85); an enclosure (84) and an upper cover (87), the enclosure (84) surrounding the turntable (85), and the upper cover (87) being disposed at an upper opening of the enclosure (84); and a mixing mechanism (5) disposed below the turntable (85), wherein when the turntable (85) rotates and a reaction vessel (57) at any of the bearing positions (86) is above the mixing mechanism (5), the mixing mechanism (5) lifts the reaction vessel (57) and rotates.

The invention belongs to the technical field of immunoassay and biosensing, and provides a novel construction mode of a heterojunction dual-photoelectrode photoelectric sensing strategy and a preparation method of an in-situ heterojunction MIO photocathode. The invention relates to a preparation method of a heterojunction dual-photoelectrode type photoelectrochemical sensor based on multi-ball cascade DNA reaction amplification. Specifically, the design is composed of an In2O3 / CdS photoanode and an in-situ formed MIL-68 / In2O3 (MIO) photocathode. The heterojunction dual-photoelectrode sensing strategy effectively prolongs an electron-hole transmission path, enhances photo-generated charge separation, and realizes' super-open 'PEC signal output, thereby providing a steady baseline for signal conversion. Meanwhile, a multi-sphere cascade DNA reaction is combined to output a three-dimensional DNA net structure to provide a large number of sites for embedding of a PEC quenching probe doxorubicin-ferrocene carboxylic acid (DOX-FcCOOH), obvious PEC signal quenching is realized by utilizing the efficient competitive electron transfer capability of the three-dimensional DNA net structure, and finally, a signal conversion system with'opening-super opening-closing 'multistage response characteristics is constructed. The detection of the nucleic acid marker Pax-5a gene of the B cell acute lymphatic leukemia is realized by detecting target objects with different concentrations.

The invention belongs to the technical field of immunoassay and biosensing detection, and particularly relates to a preparation method and application of an antibiotic electrochemical luminescence sensor based on a double-enhancement strategy. According to the invention, a bifunctional metal organic gel is used as a substrate, a graphite-phase carbon nitride quantum dot is used as a luminous body, and a self-assembly split aptamer walker is combined to construct the electrochemical luminescence sensor for sensitive detection of antibiotics. The sensor has the advantages of extremely high specificity and sensitivity, wide detection range, low detection limit and strong anti-interference performance, and has important scientific significance and application value in detection of antibiotic residues in the environment.

The invention relates to the technical field of immunoassay, in particular to a full-automatic single-molecule fluorescence immunoassay analyzer which comprises an analyzer body and a sample adding bin and further comprises a plurality of transfer pipes located in the sample adding bin. The knocking rods are located in the sample adding bin, the knocking rods are used for knocking and vibrating the bottom of the transfer pipe, when the full-automatic single-molecule fluorescence immunoassay analyzer is used, the existence of bubbles can interfere with reading of fluorescence signals, so that an analysis result is inaccurate, the bubbles are discharged through knocking and vibrating, and the accuracy of the analysis result is improved. According to the method, accurate reading of fluorescence signals can be ensured, so that the analysis accuracy is improved, the fluorescence signal intensity is optimized, bubbles can shield part of the fluorescence signals, the signal intensity is weakened, after the bubbles are discharged, the fluorescence signals can be more fully detected, the signal intensity is optimized, and the result is clearer.

The utility model relates to the technical field of fluorescence immunoassay analyzers, and discloses a reagent card positioning device of a fluorescence immunoassay analyzer, which comprises a detector, a reagent card is arranged on the outer side of the detector, clamping grooves are arranged on two sides of one end, close to the detector, of the reagent card, and a clamping component is fixedly connected inside one end, close to the reagent card, of the detector. The clamping assembly comprises a fixedly connected clamping block inserted into the end, close to the reagent card, of the detector, a first spring is fixedly connected to the middle of the end, inserted into the detector, of the clamping block, and the end, away from the clamping block, of the first spring is fixedly connected to the inner side of the detector; the separation assembly comprises a separation block arranged on one side, far away from the reagent card, of the clamping block, so that when the reagent card is inserted into the detector for detection, the detector can quickly clamp and position the reagent card by using the clamping assembly, and therefore, the stability of the detector after insertion is improved, and subsequent detection is facilitated.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention belongs to the technical field of treatment equipment, and relates to a reagent treatment device of a single molecule immunity analyzer, which comprises a treatment box, the upper part of the treatment box is open, and a reagent disc is arranged in the opening; the device comprises a fixed seat, a rotating shaft, a moving rod, a rotating assembly, a lifting assembly, an oscillating assembly and a clutch assembly. When the movable rod is driven to move upwards, the movable rod is connected with the fixed seat to drive the fixed seat to rotate so as to perform centrifugal treatment on a reagent, and when the movable rod is driven to move downwards, the movable rod is connected with the oscillation assembly to drive the fixed seat to perform circumferential oscillation so as to perform vortex treatment on the reagent; the time interval between each centrifugation and vortex can be kept consistent, the sedimentation uniformity is improved, the time required by the whole process can be shortened, sedimentation particles are prevented from caking, uniform mixing during vortex is more thorough, and the accuracy and repeatability of detection are guaranteed.

The invention relates to the technical field of fluorescence immunoassay, and discloses a quantum resonance fluorescence immunoassay medical detection method, which comprises the following steps: 1, carrying out fixation and permeation treatment on cells, respectively incubating the cells by using labeled different quantum dot-antibody compounds, specifically binding antibodies with corresponding target proteins in the cells, and detecting the target proteins in the cells; a labeled cell sample is obtained; 2, under a confocal microscope, laser with a specific wavelength is adopted to excite the marked cell sample, and fluorescence lifetime image data of different quantum dot emission wavelengths are collected; according to the method, the fluorescence lifetime image data of different quantum dot emission wavelengths are acquired through the confocal microscope, the position and content information of different target proteins can be clearly distinguished, simultaneous analysis and accurate positioning of multiple target proteins are realized, and an efficient means is provided for multi-protein research in a complex biological system.

The invention discloses a preparation method of Fe3O4 magnetic nanoparticles rich in carboxyl groups on the surface, which comprises the following steps: modifying Fe3O4 nanoparticles by using polyethylene glycol and silicon dioxide to enhance the dispersity and biocompatibility of the magnetic nanoparticles, modifying the surfaces of PEG-Fe3O4 coated SiO2 nanoparticles by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface, and modifying the magnetic nanoparticles rich in carboxyl groups on the surface by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface. Then octavinyl-POSS and double bonds on the surfaces of the nano particles are subjected to click modification to form an octahedral cage, 4-mercaptobenzoic acid and double bonds are further subjected to click modification, the surfaces of the nano particles are rich in carboxyl, and a polymer brush rich in carboxyl is constructed. Compared with a conventional streptavidin coupling method, a high-density polymer chain provides a large number of carboxyl active binding sites, so that the streptavidin coupling amount is increased, the antibody coupling rate is increased, and a solution is provided for applications such as high-sensitivity biological detection, efficient targeted drug delivery and stable immunoassay.

The invention belongs to the technical field of immunoassay and biosensing, and provides a preparation method of a photoelectrochemical sensor based on CuO-induced photocurrent polarity reversal. The invention provides a preparation method of the photoelectrochemical sensor based on CuO-induced photocurrent polarity reversal. Specifically, hollow polyhedral flower-shaped CuO with a unique inner cavity structure is adopted as a polarity induction factor, so that multiple light refraction and scattering effects are remarkably enhanced, and the light capture efficiency is improved. Besides, when CuO is introduced to the surface of the In2O3 / ZnCdS electrode as a photocurrent polarity reversal factor, due to the energy band structure difference between CuO and In2O3 / ZnCdS, the migration path of a photon-generated carrier is changed, and the photocurrent polarity is reversed from an anode to a cathode photocurrent, so that the detection specificity of the gastrin-releasing peptide is improved. The photocurrent polarity reversal strategy effectively eliminates potential false positive signal interference, and the accuracy and sensitivity of detection are remarkably improved. According to different photoelectrochemical signal intensities of to-be-detected substances with different concentrations, the gastrin-releasing peptide is detected.

The invention belongs to the technical field of biochemistry, and particularly relates to a trihexylamine artificial hapten, an artificial antigen as well as a preparation method and application of the trihexylamine artificial hapten and the trihexylamine artificial antigen. The molecular structural formula of the trihexylamine artificial hapten is as shown in a formula (I), and the molecular structural formula of the trihexylamine artificial antigen is as shown in a formula (II). The trihexyle artificial antigen can be applied to the field of preparation of anti-trihexyle antibodies. According to the present invention, the trihexylamine artificial hapten maximally retains the characteristic structure of the trihexylamine, has the active group capable of being coupled with the carrier protein, and can be used as the antigenic determinant; the further prepared trihexylamine artificial antigen can be immunized to obtain an anti-trihexylamine antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing a New Zealand white rabbit reaches up to 1: 256000, and the trihexylamine artificial antigen can be used for rapid and accurate immunodetection and immunoassay of trihexylamine.

The invention relates to the technical field of antibody detection methods, in particular to a diagnosis method and a detection system based on autoimmune disease antibody detection, and the method comprises the following steps: S1, an analysis module obtains electrochemistry related information and light reflection related information through electrochemical analysis and light reflection analysis; related information of electrochemistry and related information of light reflection are transmitted to the control module; s2, the control module obtains an antibody concentration index according to the electrochemistry related information and the light reflection related information, and transmits the antibody concentration index to a diagnosis module; s3, the diagnosis module obtains strong or weak immunoreaction information according to the antibody concentration index, and transmits the strong or weak immunoreaction information to the communication module; and S4, the communication module transmits strong or weak immunoreaction information to the user side. Immunoassay is combined with electrochemical analysis and light reflection analysis for detection, and the accuracy and comprehensiveness of detection can be improved by analyzing in different directions and combining results.

The invention relates to an immunoassay analyzer. Comprising a sampling mechanism, the sampling mechanism comprises a sampling piece, included angles are formed between every two of a first direction, a second direction and a third direction, and the sampling piece can do linear motion in the first direction and the third direction; the transfer mechanism comprises a gripper, and the gripper can do linear motion in the first direction and the third direction; the consumable mechanism comprises a bearing assembly used for containing a sample and a reagent, and the bearing assembly can do linear motion in the second direction; and the input mechanism comprises an input piece used for bearing the reactor, and the input piece can do linear motion in the second direction. Therefore, the structure of the immunity analyzer can be simplified, so that the manufacturing cost and the volume of the immunity analyzer are reduced.

Provided is an ultrasensitive method for measuring an anti-drug antibody that is simpler and less expensive than conventional methods. Provided is an ultrasensitive method for measuring an analyte using a capture probe and an assist probe and adopting an improved PALSAR method. By using the capture probe and the assist probe and adopting the improved PALSAR method in a double antigen bridging immunoassay, ultrasensitive measurement of an anti-drug antibody can be performed simply and inexpensively.

The invention discloses an anti-acrylamide derivative nano antibody mutant with mature affinity and application of the anti-acrylamide derivative nano antibody mutant. A saturated mutation library is constructed on the basis of a wild type anti-acrylamide derivative nano-antibody, an anti-acrylamide derivative nano-antibody mutant with mature affinity and remarkably improved performance is obtained through screening by a phage display method, the IC50 of the nano-antibody mutant is 0.81 mu g / mL and is improved by 55% compared with that of the wild type nano-antibody, and the anti-acrylamide derivative nano-antibody mutant has the advantages of being high in specificity, high in sensitivity and good in stability. The lowest detection limit is 0.052 [mu] g / mL, and the linear range is 0.172-9.793 [mu] g / mL; the nanometer antibody mutant can specifically recognize an acrylamide derivative, the tolerance to an organic solvent is also remarkably improved and is nearly doubled compared with that of a wild type, and the stability is higher; when being used for an enzyme-linked immunosorbent assay method for detecting acrylamide, the enzyme-linked immunosorbent assay method is accurate in detection result and high in stability, and has important significance for detecting the content of acrylamide in food.

The present disclosure relates to predicting genomic instability status in biological samples using machine learning techniques with comprehensive genomic and immune profiling (CGIP) data. Particularly, aspects are directed towards performing a genomic instability test on a biological sample. Then, multi-omics data for the subject are obtained by DNA sequencing and RNA sequencing assays, including genomic alteration data for a first set of genes and expression data for a second set of immune genes. The multi-omics data are input into a machine learning model having a tree-based architecture, which is configured to analyze features by traversing paths from root nodes to terminal nodes in each tree based on values generated from the data. The model predicts a genomic instability status, which is then provided via a user interface notification or as part of a testing report.

The invention provides a safrole hapten and application of the safrole hapten in detection of safrole through immunoassay. The structure of the safrole hapten is shown in the specification. The two safrole haptens provided by the invention have spacer arms with proper lengths, and after the safrole haptens are coupled with carrier protein, the haptens can be fully exposed, and recognition of the spacer arms can be avoided as much as possible. The safrole hapten is used for preparing an artificial antigen and an antibody, and the obtained safrole monoclonal antibody is high in titer, strong in specificity and high in affinity. An immunoassay method constructed on the basis of the safrole artificial antigen and antibody has high specificity and sensitivity, the lowest detection limit LOD is 0.09 ng / mL, the half inhibitory concentration IC50 is 2.05 ng / mL, the quantitative detection range is 0.28-14.89 ng / mL, no cross reaction exists on safrole analogues, and rapid qualitative and quantitative detection can be conducted on safrole in a sample.

The utility model discloses a sample rack separation mechanism, a sample conveying module and an immunoassay analyzer. According to the sample frame separation mechanism disclosed by the invention, the avoiding hole is formed in the sample introduction channel, and the reset piece used for driving the hook piece to be kept at the hooking position is arranged on the rotatable hook piece; therefore, the avoiding hole and the resetting piece on the sample injection channel are ingeniously utilized to control the hooking piece to be located at the hooking position, and under the driving of the separation driving device, the hooking piece is ingeniously controlled to rotate and move to the retreating position by utilizing the limiting of the sample injection channel. Therefore, a driving device specially used for driving the hooking piece to be switched between the hooking position and the returning position is not arranged, the structure and control of the sample frame separating mechanism can be simplified, and the cost of the sample frame separating mechanism is effectively reduced.

The invention relates to the field of in-vitro diagnostic equipment, and provides a pipetting system and an immunoassay analyzer, the pipetting system comprises a pipetting vertical driving assembly and a pipetting crevasse assembly, the pipetting vertical driving assembly comprises a first driving structure, a first ball screw structure, a first compression spring, a spring limiting sleeve, a nut adjusting mounting block, a guide adjusting pin, a first separation blade, a first sensor and a first mounting seat, and the first ball screw structure comprises a first screw rod, a first screw rod nut and a ball; the pipetting crevasse assembly comprises an injection mounting frame and an injection pump, and a pump head of the injection pump is used for loading a disposable TIP head. When the disposable TIP head is loaded, the loading force pressed on the disposable TIP head is buffered by the first compression spring, and the precise control of the loading force is completed through the cooperation of the first separation blade and the first sensor, so that the technical problem that in the prior art, the loading is unsuccessful due to the too small loading force or the disposable TIP head is crushed due to the too large loading force is solved.

The invention belongs to the technical field of medical instruments, and relates to a magnetic separation cleaning method, a magnetic cleaning device and an immunity analyzer, and the method comprises the following steps: transferring a reaction cup among a liquid injection station, an adsorption station and a liquid suction station; cleaning liquid is injected into the reaction cup at the liquid injection station; magnetic particle conjugates in the reaction cups are adsorbed to the inner walls of the reaction cups at the adsorption station, and waste liquid is extracted from the reaction cups injected with the cleaning liquid at the liquid suction station. After the steps are adopted, the magnetic separation cleaning device has the beneficial effects that by reasonably planning the number of the liquid injection stations and the number of the liquid suction stations and matching of the cleaning orders of magnetic separation cleaning, the structure of the magnetic separation cleaning device is effectively simplified, the size of the device is reduced, the manufacturing cost is reduced, and the cleaning efficiency of the magnetic separation cleaning device is improved.

The invention belongs to the technical field of immunoassay rapid detection, and relates to a method for screening residues of traditional Chinese medicinal material aflatoxin B1 based on multicolor evolution fluorescence immunochromatography, which comprises the following steps: coupling red quantum dot fluorescent microspheres with an AFB1 antibody to prepare a detection probe; coupling or mixing the green quantum dot fluorescent microspheres with an AFB1 antigen to prepare an internal standard probe; respectively coating an internal standard probe and goat anti-mouse IgG on an NC membrane to complete preparation of a T line and a C line; preparing a test solution containing a detection probe, dripping the test solution on a test paper sample pad, and observing fluorescence colors of a T line and a C line by ultraviolet; and judging whether the residual quantity of the traditional Chinese medicine AFB1 exceeds the limit specification or not according to the T-line fluorescence color. By introducing the internal standard probe, a ratio fluorescence immunoassay system is constructed, so that the sensitivity and the accuracy of an analysis result can be improved, and the interference of a sample matrix is reduced; the used detection test paper is small in matrix influence and wide in application range, and can be used for rapid limited screening of AFB1 residues in traditional Chinese medicinal materials.

The invention provides multi-channel multi-joint inspection POCT full-automatic chemiluminescence equipment. The equipment comprises a rack; the sampling gun head accommodating mechanism, the test tube accommodating mechanism and the HGB detection mechanism are arranged on the rack in a longitudinal reciprocating motion manner, and a treating fluid container is arranged on the sampling gun head accommodating mechanism; the sampling mechanism is arranged on the rack in a transverse reciprocating motion manner; the reaction bin mechanism can be longitudinally arranged on the rack in a reciprocating manner and comprises a reaction bin body used for loading multiple joint inspection reagent strips, and the reaction bin body is provided with pressing elastic pieces used for pressing the multiple joint inspection reagent strips; the clamping and locking mechanism is located below the reaction bin mechanism, the reaction bin mechanism further comprises a driving device and a synchronous conveying belt connected with the driving device and a reaction bin body, and the clamping and locking mechanism is used for clamping, locking or loosening and unlocking the synchronous conveying belt so as to lock or unlock the reaction bin body. According to the present invention, the device has the immunoassay function, the HGB detection can be achieved, the multi-joint detection reagent strip does not shake or tilt, and the reaction bin body can be locked.

The invention relates to a micro-fluidic chip and an immunoassay instrument. A micro-fluidic cavity is formed in a body; the microfluidic chamber comprises a first valve control structure, a second valve control structure, a third valve control structure, a fourth valve control structure, a first liquid cavity, a second liquid cavity, a first reaction cavity, a detection reaction cavity and a waste liquid cavity; the first liquid cavity, the first valve control structure, the first reaction cavity, the fourth valve control structure and the waste liquid cavity are communicated in sequence according to the distance from the rotating center from near to far, and the second liquid cavity, the second valve control structure, the detection reaction cavity, the third valve control structure and the waste liquid cavity are communicated in sequence. By designing a first liquid cavity, a second liquid cavity, a first reaction cavity and a detection reaction cavity which are matched with each other, the microfluidic chip is suitable for a delay one-step method and two-step method detection scheme of two-time reaction needing accurate quantification, so that the microfluidic chip can be matched with delay one-step method and two-step method immunoreactions; and the universality of the micro-fluidic chip, especially a disc-type micro-fluidic chip, is further expanded.

The invention relates to the technical field of machinery, and discloses a high-speed full-automatic chemiluminescence immunoassay analyzer and a control method thereof.The high-speed full-automatic chemiluminescence immunoassay analyzer comprises an immunoassay analyzer body, a dilution module is arranged in the immunoassay analyzer body, and a cleaning module is arranged in the immunoassay analyzer body; the immunity analyzer comprises a cup arranging device module, a sample arm module is arranged on the back face of the cup arranging device module, the right side of the sample arm module and a dilution module are arranged, the dilution module comprises a dilution needle, a motor, a transfer sliding block base, a dilution disc, a transfer disc, a pipetting needle, a uniform mixing mechanism A and a uniform mixing mechanism B, and the sample arm module comprises a sample needle. The transfer disc is provided with 36 cup positions in total, and three cup positions can be rotated at a time. According to the high-speed full-automatic chemiluminescence immunoassay analyzer and the control method thereof, the information of the placed reagent disc can be automatically diluted and detected, manual operation is not needed, the full-automatic detection capability is improved, and the positioning and monitoring functions of the reagent cup are enhanced.

The invention provides an immunoassay method based on instant synthesis of a nano-enzyme of a bimetallic CuCo Prussian blue analogue (CuCo PBA), and belongs to the field of chemical and biological sensing. According to the method, a sandwich type immune mode is formed by a glucose oxidase labeled antibody, a target object and an immobilized antibody, so that the concentration of the target object is in direct proportion to the amount of glucose oxidase. Hydrogen peroxide generated by catalyzing glucose by glucose oxidase catalyzes 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to oxidize and develop color under the assistance of CuCo PBA nano-enzyme (Cu < 2 + > and K3 [Co (CN) 6]) which is generated instantly (Cu < 2 + > and K3 [Co (CN) 6] are added instantly). And the color change of the detection liquid is recorded and is used for visual detection and quantitative detection by a microplate reader. The problems of advanced synthesis and storage of nano materials are avoided, the cost is low, the colorimetric detection method is simple in operation process, rapid in color development and high in sensitivity, and a novel method which is convenient and stable in performance is provided for clinical immunological detection.