210 results about "Immune profiling" patented technology

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention discloses a preparation method of Fe3O4 magnetic nanoparticles rich in carboxyl groups on the surface, which comprises the following steps: modifying Fe3O4 nanoparticles by using polyethylene glycol and silicon dioxide to enhance the dispersity and biocompatibility of the magnetic nanoparticles, modifying the surfaces of PEG-Fe3O4 coated SiO2 nanoparticles by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface, and modifying the magnetic nanoparticles rich in carboxyl groups on the surface by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface. Then octavinyl-POSS and double bonds on the surfaces of the nano particles are subjected to click modification to form an octahedral cage, 4-mercaptobenzoic acid and double bonds are further subjected to click modification, the surfaces of the nano particles are rich in carboxyl, and a polymer brush rich in carboxyl is constructed. Compared with a conventional streptavidin coupling method, a high-density polymer chain provides a large number of carboxyl active binding sites, so that the streptavidin coupling amount is increased, the antibody coupling rate is increased, and a solution is provided for applications such as high-sensitivity biological detection, efficient targeted drug delivery and stable immunoassay.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

This application relates to a detection reagent, kit, and method that simultaneously improves sensitivity and broadens the detection range in competitive immunoassay. The reagent includes a detection antibody bound to luminescent microspheres that specifically binds to analyte molecules in the sample, and two sets of labeled antigens containing the same competing antigen and labeled molecules of different molecular weights bound to it. The competing antigen competes with the analyte molecule for binding to the detection antibody, and the affinity of the competing antigen for binding to the detection antibody is higher than that of the analyte molecule for binding to the detection antibody. The technical solution of this application, by using two sets of labeled antigens with different affinities, ensures that the labeled antigens that play a dominant role in samples with varying concentrations of analyte molecules differ. This is suitable for competitive immunoassay of small molecule antigens or haptens in both low-concentration and high-concentration samples, and can broaden the detection range while ensuring high functional sensitivity.

This application provides an immunoassay analyzer, a magnetic separation device, and a method for cleaning the aspiration needle, belonging to the technical field of immunoassay equipment. The immunoassay analyzer includes a magnetic separation device, which includes a magnetic separation disk, a liquid injection device, an aspiration needle, and a controller. It also includes a separation disk heating module, or a separation disk heating module and a cleaning agent heating module. The magnetic separation device has a first mode and a second mode. In the first mode, it can clean the magnetic particles in the reaction cup. In the second mode, the controller can control the heating module to heat the aspiration needle cleaning agent to a first temperature. The aspiration needle cleaning agent has high activity and cleaning ability at the first temperature, and can efficiently clean the deposits attached to the inner wall of the aspiration needle.

This invention relates to a hybridoma cell line secreting a monoclonal antibody against pendimethalin and its application, belonging to the field of immunoassay. The hybridoma cell line secreting the pendimethalin monoclonal antibody of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46744. The pendimethalin monoclonal antibody secreted by this hybridoma cell line is used for the analysis and detection of pendimethalin residues in food safety testing. The pendimethalin monoclonal antibody cell line obtained by this invention can be used for immunoassay detection, exhibiting good detection sensitivity and specificity (IC50) for pendimethalin. 50 The value was 1.87 ng / mL.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

This invention belongs to the field of immunoassay and biosensing technology, and discloses a method for preparing a ratiometric ECL biosensor based on Eu / Tb-Lu MOGs. Using Eu / Tb-Lu MOGs as the luminescent material and H3-tctpy and luminol as ligands, a single-luminescent material with dual ECL emission is synthesized. Lanthanide ions provide the cathode ECL emission, enhanced by the antenna effect and Tb→Eu energy transfer; the anode emits light from luminol using dissolved oxygen in water, requiring only the addition of K2S2O8 as a single co-reactant, resulting in fewer side reactions and a stable signal. The dual-potential ratiometric sensing utilizes the dual ECL signal for self-calibration, improving anti-interference and reliability; the single-luminescent dual-potential system achieves synchronous anode / cathode luminescence, simplifying the system and improving detection accuracy. By detecting changes in the ECL signal of different concentrations of analytes, the detection of microRNA-126 is achieved.

The application discloses a kind of diflufenican hapten, antigen, antibody and its preparation method and application.The application prepares diflufenican hapten as shown in formula (I) and corresponding diflufenican antigen, and specific antibody for detecting diflufenican is prepared by diflufenican antigen, and further constructs the immune analysis method for directly detecting diflufenican pesticide residue, which is particularly suitable for on-site rapid detection, and can meet the needs of regulatory authorities and detection agencies formula (I).

The application discloses a kind of filling assembly and control method, magnetic separation cleaning device.Adding component includes cup rotating member and injection needle, cup rotating member is used to grab reaction cup and drive it to rotate, and its central axis is provided with injection channel;Injection needle is coaxially arranged with cup rotating member and can be from injection channel into reaction cup.In control timing, cup rotating member first drives reaction cup to rotate, and after it is in rotating state, injection needle then adds cleaning fluid or substrate into reaction cup.The design will overlap the timing of filling and mixing steps, effectively reduce the filling splash and magnetic bead loss, improve the purification efficiency, and can complete efficient processing in the short residence time of magnetic separation carousel, especially suitable for high-throughput chemiluminescence immunoassay equipment, while ensuring detection accuracy significantly improve detection throughput.

The utility model relates to a reagent disc applied to an immunity analyzer. The reagent disc comprises a reagent disc, a fixed cover fixedly mounted at the top end of the reagent disc and a movable cover detachably mounted, a reagent pot is detachably mounted in the reagent disc, and the outer wall of the reagent pot abuts against the inner wall of the reagent disc; a reagent tray is rotatably installed in the reagent pot in a limited mode, a plurality of reagent boxes are sequentially rotatably installed on the reagent tray in the circumferential direction in a limited mode, pinions are detachably installed on the reagent boxes, fixed gears are detachably installed in the reagent pot, the fixed gears and the reagent tray are coaxially arranged, and the fixed gears are meshed with the pinions. A rotating mechanism is mounted on the reagent tray and is in transmission fit with the reagent tray; a refrigeration mechanism for cooling the interior of the reagent pot is mounted on the reagent pot; according to the chemiluminescence immunoassay kit, reagents for chemiluminescence immunoassay can be uniformly mixed and refrigerated, so that the accuracy of subsequent detection results is improved.

The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

The present invention concerns a diagnostic device and method for detecting thiamine and Vitamin A deficiencies using salivary biomarkers. The device utilizes a lateral flow assay to detect secretory Immunoglobulin A (sIgA) and amylase in saliva, providing a non-invasive, easy-to-use, and reliable method for assessing nutritional deficiencies. This invention is particularly useful for monitoring health status in populations with limited access to medical facilities.

The application relates to a preparation method of a Pt single-atom nanoscale enzyme and application of the Pt single-atom nanoscale enzyme in detection of cardiac troponin I, and the preparation method comprises the following steps: 1) ultrasonic dispersion of cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized, grinding the solid mixture into a powder; 2) performing Joule heat treatment on the powder in a heating device under an air atmosphere, and performing washing and vacuum drying on the reactants to obtain the Pt single-atom nanoscale enzyme. In the one-step chemical reaction process, Se doping is introduced to construct oxygen vacancies, rapid heating and structure shaping are realized by combining the Joule heat treatment, a Pt single-atom catalyst loaded with Se-doped Co3O4 is obtained, the oxygen vacancies and the Se doping sites are used as anchor sites in cooperation, the Pt single atoms are effectively fixed, and the loading capacity and the dispersity of the Pt single atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The utility model provides a fluorescence immunoassay analyzer, which is used for detecting a reagent card and comprises an analyzer body and a positioning block, the analyzer body is provided with an inlet / outlet and an accommodating groove communicated with the inlet / outlet; the positioning block is arranged in the accommodating groove in a penetrating manner and is detachably connected to the analyzer body; the positioning block is provided with a clamping groove and an elastic buckle, and the reagent card can move along the clamping groove and is clamped with the elastic buckle. According to the utility model, the positioning block provided with the elastic buckle is detachably connected to the analyzer body, so that when the elastic buckle is subjected to structural fatigue or damage due to frequent insertion and extraction of the reagent card, the analyzer body does not need to be disassembled, and only the positioning block needs to be taken out of the accommodating groove for replacement, thereby simplifying the maintenance process, shortening the maintenance time and improving the maintenance efficiency. The risk of secondary damage to precise detection elements in the analyzer in the disassembling process is also avoided, the maintenance efficiency of equipment is improved, and the service life of the equipment is prolonged.

The invention discloses a handheld fluorescence immunoassay analyzer with a dustproof function, and aims to solve the problem that dust and stains easily enter an existing handheld fluorescence immunoassay analyzer from a test paper insertion hole, so that the accuracy of a detection result is influenced. The analyzer comprises an analyzer body and a test paper insertion hole formed in one end of the analyzer body, a dustproof assembly is arranged at the end, close to the outer side of the analyzer body, of the test paper insertion hole, the dustproof assembly comprises a dustproof plate, a driving part and a stirring part, and the dustproof plate can elastically ascend and descend in the test paper insertion hole. During use, the stirring piece is rotated to drive the driving piece to enable the dustproof plate to open the test paper insertion hole, fingers do not need to contact the dustproof plate, insertion of test paper cannot be hindered, and the test paper insertion hole is closed after detection is completed, so that dust is effectively prevented from entering the analyzer in the detection process and after detection is completed; the accuracy of a detection result is improved.

The present invention relates to an immunoassay chip and, more particularly, to an immunoassay chip having improved specimen detection sensitivity using a scattered-light signal of a label material.

The invention discloses a fluorescence immunoassay analyzer test fixture and a test method thereof, and belongs to the technical field of medical instrument tests, the fluorescence immunoassay analyzer test fixture comprises a first assembly body and a second assembly body movably connected with the first assembly body; the first assembly body is of a plate-shaped structure, the second assembly body is of a hollow box-shaped structure with a top cover and without a bottom cover, and the first assembly body is matched with the second assembly body; the first assembly body is of a double-layer structure and comprises a bottom plate and a fixing plate arranged on the bottom plate. Wherein the fixing plate is provided with a plurality of fixing grooves and fixing columns which are matched with the fluorescence immunoassay analyzer; clamping grooves matched with the second assembly bodies are further formed in the four corners of the fixing plate. The fluorescence immunoassay analyzer is accurately fixed and positioned through detachable connection of the second assembly body and the first assembly body, and the technical effects of simplifying the test operation process and improving the test stability and safety are achieved.

The present application belongs to the technical field of bio-chemical industry, and particularly relates to a lorcaserin artificial hapten, a lorcaserin artificial antigen, and a preparation method and application thereof. The lorcaserin hydrochloride is used as a starting material for synthesizing the artificial hapten, the lorcaserin artificial hapten of the present application retains the characteristic structure of lorcaserin to the greatest extent, and has an active group which can be coupled with a carrier protein, and can be used as an antigenic determinant; the lorcaserin artificial antigen prepared further can be used for immunization to obtain an anti-lorcaserin artificial antibody with high affinity, high sensitivity and high specificity, the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:256000, and the immune serum can be used for rapid and accurate immunodetection and immunological analysis of lorcaserin.

This invention relates to a medemididine hapten, a complete antigen, and their preparation methods and applications. The structure of the medemididine hapten is shown in Formula I. The preparation method of the medemididine hapten includes: (1) substituting medemididine with ethyl 3-bromopropionate to obtain intermediate 2; (2) adding sodium hydroxide to deprotect the ester group to obtain medemididine hapten 3. The structure of the complete antigen is shown in Formula II. The preparation method of the complete antigen includes: coupling the medemididine hapten with a carrier protein to obtain the complete antigen. This invention discloses a method for preparing medemididine hapten and complete antigen. The synthesis steps are simple and effective, and it can be used in immunoassay. Among them, colloidal gold immunochromatography is simple to operate, has a short detection time, high specificity, and good reproducibility, which can meet the domestic demand for immunoassay of medemididine.

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

The application discloses a kind of full-automatic chemiluminescence immunoassay analyzer, it is related to immunoassay analyzer technical field, the analyzer includes main casing and front casing, the front casing is set in the side end of main casing, the inside of main casing is provided with reaction disc, the periphery of reaction disc is provided with conveying assembly, sampling assembly, gripper assembly, photometric assembly, mixing assembly and magnetic separation component, compared with the analyzer of current setting reagent processing unit and its supporting isolation component, high-precision sample reagent storage environment is constructed by isolation component, fundamentally solve the problem that traditional analyzer is easily polluted by outside and energy consumption is too high in non-working state, in addition, the linkage of driving assembly, isolation component and collaborative component in the application ensures that reagent processing unit has fault redundancy capability, so that equipment can still complete current detection process when local fault, effectively improve work efficiency.

This invention discloses a shake-type fully automated chemiluminescence immunoassay analyzer, belonging to the field of immunoassay analyzer technology. It includes a main body and a display screen. The main body has a detection port at its end, with a detection chip placed on the surface of the port. A groove is formed on the surface of the main body, and a placement assembly is located inside the groove. The placement assembly is used to place multiple sets of solution bottles to be tested. A sealing plate is provided on the surface of the groove, and a clamping assembly is provided on the surface of the placement assembly. This invention controls the output of a servo motor to drive a main gear to rotate coaxially. When the main gear rotates, it drives a secondary gear to mesh and rotate. When the secondary gear rotates, it drives a locking post to rotate coaxially. When the locking post rotates, it drives a first connecting plate to rotate coaxially. At this time, a second connecting plate located above the first connecting plate rotates coaxially under the action of a buffer assembly, thereby achieving centrifugal rotation of the solution bottles placed inside the placement seat, ensuring uniform mixing of the internal solutions.

Provided is an automatic analysis device capable of achieving reagent suction not affected by atmospheric pressure and stabilizing a reagent supply amount and a residual reagent amount. Provided are a suction nozzle (suction part) 3, a discharge nozzle (discharge part) 4, a syringe 10, and a control part 102 for controlling and driving the syringe 10 to suck liquid from a first container 1 through the suction nozzle 3 and to discharge the liquid sucked through the suction nozzle 3 into a second container 2 through the discharge nozzle 4. The control part 102 controls suction time per session of the suction nozzle 3 from the first container 1 on the basis of the outside atmospheric pressure or the altitude of an immunoassay device (automatic analysis device) 101.

The invention discloses a high-sensitivity wide-spectrum specific antibody for resisting parathion, parathion-methyl and fenitrothion, the high-sensitivity wide-spectrum specific antibody comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO: 2; the amino acid sequence of the light chain variable region coding gene is shown as SEQ ID NO: 4. The invention also provides an antibody expression plasmid. The obtained variable region sequence gene is respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a new recombinant full-length antibody is successfully obtained through an HEK293 (F) mammalian cell expression system. Based on the characteristics of stability and easy preservation of recombinant expression plasmids, the recombinant expression plasmids can be used for multi-batch repeated production of prohibited and limited organophosphorus pesticide recombinant antibodies for resisting parathion and the like; a stable and reliable core reaction reagent is provided for construction of an immunoassay method for multiple residues of organophosphorus pesticides such as parathion in a development environment and a food sample and development of a field screening product.

The embodiment of the present application relates to the field of immunoassay analyzer, and discloses a liquid sucking method, a storage medium, an electronic device and an immunoassay analyzer, the method comprising: acquiring an image of a target test tube carrying a liquid; identifying the test tube type of the target test tube based on the image; determining the effective height threshold corresponding to the target test tube based on the preset correspondence relationship between the test tube type and the effective height threshold; and detecting the liquid level of the liquid in real time during the movement of the liquid sucking tool from the top of the target test tube to the liquid surface, wherein the effective height threshold is used as one of the liquid level detection standards of the liquid. The problem that the prior art cannot meet the requirement of customers for a lower dead space amount can be solved.

Aspects of the disclosure relate to methods, systems, and computer-readable storage media, that are useful for characterizing subjects having cancer. The disclosure is based, in part, on methods for immunoprofiling a cancer subject and the subject's prognosis and / or likelihood of responding to an immunotherapy based upon analysis of leukocyte populations in the peripheral blood of the subject.

The application discloses a kind of natural product antiviral drug screening method based on hexagon protein and application, belong to active ingredient screening and antiviral drug development field.The method includes: preparation nitrogen-doped mesoporous carbon and silver nanosheet composite solution, drop coating on the surface of pretreated glassy carbon electrode, after drying by activation, hexagon protein incubation, blocking and enzyme label antibody reaction, construct electrochemical sensing electrode;With the electrode, the sample to be screened natural product is incubated, and the current response signal is detected;According to signal change combination inhibition rate formula evaluation antiviral activity.The application takes hexagon protein as recognition target, based on the principle of competitive immunoassay, realizes the specific, high sensitivity detection of antiviral active ingredient in natural product, with the advantages of fast operation, low cost, small sample consumption, high throughput, etc., provides effective technical support for the mining of antiviral active ingredient in natural product such as traditional Chinese medicine.