820 results about "Immune profiling" patented technology

An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and a second immunosensor that acts as an immuno-reference sensor and generates a signal that is the same as or predictably related to the degree of non-specific binding which occurs in the region of the first immunosensor, and has an immunocomplex between an immobilized antibody and an endogenous or exogenous protein that is in the sample and that is not the target analyte.

The invention relates to an enzyme-linked immune analysis method and a fully-automatic enzyme-linked immune analyzer. The analyzer comprises a rack component, a washing component, a sample adding/reagent adding component, a heating and temperature control component, a fluid path system, an input and output device, an optical measurement component and a control system, wherein the rack component comprises a rack soleplate; a pillar is arranged on the rack soleplate; a lower fixed plate and an upper fixed plate are arranged on the pillar from bottom to top in sequence; a circular reaction plate is arranged between the lower fixed plate and the upper fixed plate; the circular reaction plate is connected with the upper fixed plate and the lower fixed plate through a central shaft; a first motor is fixedly arranged at the lower part of the lower fixed plate; and the first motor is used for driving the circular reaction plate to rotate through a first transmission mechanism. When in use, the enzyme-linked immune analysis method and the fully-automatic enzyme-linked immune analyzer can carry out the detection of corresponding projects without wasting reagent through only one sample; and detection reagents need not to be respectively contained in different reagent bottles, so that the operation is very convenient, and the operation error is not easily caused, thereby ensuring the accuracy of the detection results.

The invention relates to a full-automatic fluorescent quantitative immunity analyzer and a method, and belongs to the technical field of detection. The full-automatic fluorescent quantitative immunity analyzer comprises a sample feeding device, a hatching device, a sampling device, a data acquisition device, a reagent card device and a system control module; the purpose of automatic quantitative immunity analysis is achieved through organic combination of all the devices and modules and mutual cooperation of all elements. According to the fluorescent quantitative immunity detection method, the full-automatic fluorescent quantitative immunity analyzer is adopted, therefore, full-automatic instrumental analysis is achieved, the detection efficiency is improved, and the professional requirement on an operator is lowered.

The invention discloses an application of nano-gold directly bonded with luminol in an immunoassay. The invention is characterized in that the immunoassay probe of a nano-gold directly bonded with luminol comprises antibodies which are labeled by the nano-golds directly bonded with luminol, wherein the nano-gold directly bonded with luminol are prepared through reducing chloroauric acid by the luminol at a single step; a chemiluminescence immunoassay method is based on the immunoassay probe of the nano-gold directly bonded with luminol; and a kit is used for carrying out the immunoassay method. The chemiluminescence immunoassay method of the invention has the advantages of high sensitivity (for instance, the detection limit can reach 1.0pg/mL for detecting human IgG), wide range of linearity, good repeatability, simple operation, low cost and the like, can be applied to detect multi-analyte in various samples and has key application prospect in fields, such as clinical diagnosis and cure, drug analysis, food security detection, environmental monitoring and the like.

The invention relates to the immunoassay medical field, particularly provides a magnetic particle competition method chemiluminescence immunoassay assay kit and a preparation method thereof used for detecting hormones. The kit according to the invention comprises: 1) a calibrator; 2) magnetic particles which are coated with streptavidin; 3) hormone antigens of enzyme markers; and 4) a chemiluminescence substrate. Further, the method for preparing the kit according to the invention has the following steps: 1) pure raw materials are used to prepare the calibrator; 2) the antigens are used to coat the magnetic particles; 3) the antigens of the enzyme markers are prepared; 4) the calibrator, the chemiluminescence substrate and the antigens of the enzyme markers are packaged in a separated way; and 5) a finished product is packaged. The kit has the advantages of convenience, rapidness, sensitivity, stability, and the like.

The invention relates to an immunoassay detection kit, and provides an allergenic specific IgE antibody immunoassay detection kit and a preparation method thereof. The detection kit comprises magnetic microspheres coupled with allergenic protein, magnetic microspheres coupled with anti-human IgE antibody, a human IgE antibody standard substance, alkali phosphatase labeled anti-human IgE antibody and an alkali phosphatase chemical luminous substrate AMPPD. The preparation method for the detection kit comprises the following steps: coupling the allergenic protein to the magnetic microspheres, coupling the anti-human IgE antibody to the magnetic microspheres, preparing the human IgE antibody standard substance, preparing the alkali phosphatase labeled anti-human IgE antibody, preparing the alkali phosphatase chemical luminous substrate AMPPD and packing the combination. The detection kit has the advantages of high sensitivity, high accuracy, quick response, convenient usage, low dosage and low cost, can assist in precisely and quantitatively measuring sensitive allergene, and guides clinical anaphylactic reaction treatment.

Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.

The invention relates to an electrochemical immunosensor, a preparation method and application thereof, in particular to a sandwich type electrochemical immunosensor constructed by a marker based on dumbbell type Pt-Fe3O4 nano particles serving as a second antibody, a preparation method thereof, and application of the electrochemical immunosensor prepared by using the method in measurement of tumor markers. The electrochemical immunosensor is high in accuracy, stability, reproducibility and sensitivity, can quickly and conveniently perform immunoassay detection and can be used for clinical analysis.

The invention discloses a sealing and stabilizing agent for a microporous board. In terms of 100ml water, 0.1-3g of protein, 3-20g of saccharide, 0.01-1g of organic polymer, 0.01-0.05ml of nonionic surfactant, 0.01-0.1g of preservative and 10-100mmol of buffer solution are contained. The sealing and stabilizing agent disclosed by the invention is researched and designed on account of the characteristic that antigen or antibodies coated on a stationary phase are easy to inactivate. The sealing and stabilizing agent includes not only sealing components but also organic components and inorganic components, wherein the sealing components can be used for effectively sealing excessive loci on a sealing board, and the organic components and the inorganic component can be used for effectively stabilizing the antigen or the antibodies, so that the sealing purpose and the stabilizing purpose can be organically combined together through one-step operation. The sealing and stabilizing agent disclosed by the invention can be applied to immunological detection methods such as enzymelinked immunosorbent assay, chemiluminesent immunoassay, time resolved fluoroimmunoassay and the like, wherein the microporous board serves as the stationary phase; and the sealing and stabilizing agent can be used for effectively stabilizing the activity of the antigen or the antibodies coated on a stabilizing board.

Systems and methods that analyze blood-based cancer diagnostic tests using multiple classes of molecules are described. The system uses machine learning (ML) to analyze multiple analytes, for example cell-free DNA, cell-free microRNA, and circulating proteins, from a biological sample. The system can use multiple assays, e.g., whole-genome sequencing, whole-genome bisulfite sequencing or EM-seq, small-RNA sequencing, and quantitative immunoassay. This can increase the sensitivity and specificity of diagnostics by exploiting independent information between signals. During operation, the system receives a biological sample, and separates a plurality of molecule classes from the sample. For a plurality of assays, the system identifies feature sets to input to a machine learning model. The system performs an assay on each molecule class and forms a feature vector from the measured values. The system inputs the feature vector into the machine learning model and obtains an output classification of whether the sample has a specified property.

The invention relates to a pathogen detection method based on a micro-fluidic chip. The method adopts a micro-fluidic chip integrated with a micro-magnetic field as a reaction container, adopts magnetic balls as solid phase carriers, and adopts quantum dots modified by streptavidin (SA-QDs) as fluorescent markers; under the action of the micro-magnetic field, magnetic balls are captured at specific parts in a chip channel, and thus a micro-reaction zone is formed; pathogens are captured through a sandwich immunization reaction; with the interaction between biotin and streptavidin, the immunofluorescence quantitative analysis of the pathogens is realized. The detection method combines the micro-fluidic chip, magnetic immunoassay, and fluorescence detection, has the advantages of rapidness, high efficiency, and few sample using amount for the micro-fluidic chip, high specificity and strong manoeuvrability for magnetic immunoassay, and excellent optical properties for quantum dots, and is a multi-objective real-time pathogen detection method.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for phosphatidylinositol proteoglycan-3(GPC-3) and a preparation method thereof, and realizes the simultaneous serological detection of GPC-3 N terminal and C-terminal protein with the chemiluminescent immunoassay method. The kit has the advantages of simple sampling, convenient detection and accurate and specific technical method. The invention adopts a biotin-strapavidin system to coat antibodies and improve the efficiency of antibody coating and the linear range of detection as well as sensitivity, and can be conveniently used for the tracing observation of early diagnosis or treatment effect for primary carcinoma of liver.

This invention discloses a citrinin immune chromatography test paper and its process method and utility. The test paper comprises pad, sample pad, tracing particle compound pad, pyroxylin film, adsorption pad, test thread, and control thread. The method is the following: to orderly put sample pad, tracing particle compound pad, pyroxylin film and adsorption pad on the pad; the tracing particle compound pad has citrinin antibody with tracing particle mark; the pyroxylin film has test thread and control thread; the test thread has citrinin antigen and antibody of citrinin antigen.

The invention relates to the medical field of immunoassay, particularly provides a measuring kit of the chemiluminescence immunoassay of a CA125 magnetic particle. The kit of the invention comprises: 1) a CA125 working calibrator; 2) the mixed solution of the magnetic particle coated with a CA125 monoclonal antibody and the CA125 monoclonal antibody marked by biotin; 3) streptavidin marked by horse radish peroxidase; 4) a chemiluminescence substrate; and 5) a reaction tube. Further, the method for preparing the kit according to the invention comprises the following steps: 1) the working calibrator is prepared by CA125 pure products; 2) the mixed solution of the magnetic particle coated with the monoclonal antibody and the monoclonal antibody marked by the biotin is prepared; 3) the horse radish peroxidase is used for marking the streptavidin; 4) the chemiluminescence substrate is prepared; 5) the working calibrator, the mixed solution, the enzyme markers, the chemiluminescence substrate and the reaction tube are sub-assembled; and 6) assembly is carried out to obtain a finished product. The kit of the invention has the advantages of simpleness and convenience, fastness, sensitivity and stability and the like.

The invention relates to a reagent box for quantificationally detecting the content of fumonisin B1 in food by adopting an enzymoimmunoassay technology and the detecting method thereof. The reagent box includes the steps that a fumonisin B1-KLH antigen is prepared; a polyclonal fumonisin B1 antibody is prepared; an enzyme labeling antigen is prepared; a coated plate is prepared, and a reagent is prepared. The invention mainly adopts the enzymoimmunoassay technology to detect the fumonisin B1, can realize the aim of quantificationally detecting the content of fumonisin B1 in food and alimentary crops; and the reagent box has simple structure, convenient usage, cheap price and high sensitivity; the detecting method has strong specificity, high sensitivity, good veracity, and the sensitivity can reach gamma/kg grade.

The invention belongs to the veterinary medicine residual analysis and immunity analysis technique field and specifically relates to an enzyme-linked immunity method and the reagent kit thereof which can discern specificity monoclonal antibodies of tylosin and tilmicosin and detect the residuals of tylosin and tilmicosin at the same time; the monoclonal antibodies in the invention is secreted from hybridoma cell strain P3C4 established by the applicant; the hybridoma cell strain is preserved in China Center of Type Culture Collection; the number of preservation of the hybridoma cell strain is CCTCC No: C200719; the invention discloses the preparation method and enzyme-linked immunity detection method of the monoclonal antibody, coating antigen and immunogen. Compared with the prior art, the monoclonal antibody prepared in the invention can discern tylosin and tilmicosin at the same time and add the detecting object in the prior art. The reagent kit and the method in the invention has the advantages of simple, convenience, swiftness, sensitivity and accuracy; furthermore, the reagent kit and method in the invention can detect the residuals of tylosin and tilmicosin in an animal edibility tissue at the same time.

The invention relates to the medical field of immunoassay, and concretely provides a dissociate human chorionic gonadotrophin beta-subunit magnetic particle chemiluminescence quantitative assay kit having the advantages of simplicity, rapidness, high sensitivity, wide linear range and good stability, and its preparation method. The method combines a magnetic particle immune separating technology to apply an enzymatic chemiluminescence substrate on the basis of enzyme-linked immunosorbent assay, and allows an optical signal generated by the detection of the luminescence substrate to substitute a chromogenic substrate in enzyme immune assay, so the method has the advantages of substantially improved sensitivity, simple operation and wide practicality, can be applied to open semi-automatic chemiluminescence detectors, can also be applied to full-automatic measure systems, and can realize batch and fast detection, low use cost, and easy popularization and application.