871 results about "Immune profiling" patented technology

An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and a second immunosensor that acts as an immuno-reference sensor and generates a signal that is the same as or predictably related to the degree of non-specific binding which occurs in the region of the first immunosensor, and has an immunocomplex between an immobilized antibody and an endogenous or exogenous protein that is in the sample and that is not the target analyte.

The invention relates to the immunoassay medical field, particularly provides a magnetic particle separation chemiluminescence immunoassay assay kit and a preparation method thereof used for detecting disease related markers. The kit of the invention comprises: 1) a calibrator; 2) magnetic particles which are coated with streptavidin; 3) disease related marker antibodies of enzyme markers and biotin markers; and 4) a chemiluminescence substrate. Further, the method for preparing the kit according to the invention includes the following steps: 1) pure raw materials are used to prepare the calibrator; 2) the streptavidin is used to coat the magnetic particles; 3) the mixed liquid of the enzyme and the biotin markers are prepared; 4) the calibrator, the chemiluminescence substrate as well as the mixed liquid of the enzyme and the biotin markers are packaged in a separated way; and 5) a finished product is packaged. The kit has the advantages of convenience, rapidness, sensitivity, stability, and the like.

The invention relates to an enzyme-linked immune analysis method and a fully-automatic enzyme-linked immune analyzer. The analyzer comprises a rack component, a washing component, a sample adding / reagent adding component, a heating and temperature control component, a fluid path system, an input and output device, an optical measurement component and a control system, wherein the rack component comprises a rack soleplate; a pillar is arranged on the rack soleplate; a lower fixed plate and an upper fixed plate are arranged on the pillar from bottom to top in sequence; a circular reaction plate is arranged between the lower fixed plate and the upper fixed plate; the circular reaction plate is connected with the upper fixed plate and the lower fixed plate through a central shaft; a first motor is fixedly arranged at the lower part of the lower fixed plate; and the first motor is used for driving the circular reaction plate to rotate through a first transmission mechanism. When in use, the enzyme-linked immune analysis method and the fully-automatic enzyme-linked immune analyzer can carry out the detection of corresponding projects without wasting reagent through only one sample; and detection reagents need not to be respectively contained in different reagent bottles, so that the operation is very convenient, and the operation error is not easily caused, thereby ensuring the accuracy of the detection results.

There is provided a lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample where the lateral flow assay device has a porous membrane in communication with a conjugate pad and a wicking pad. The porous membrane has a detection zone which has an immobilized first capture reagent configured to bind to at least a portion of the analyte and analyte-conjugate complexes to generate a detection signal. A control zone may be located downstream from the detection zone on the porous membrane and has a second capture reagent immobilized within the control zone. The conjugate pad is located upstream from the detection zone, and has detection probes with specific binding members for the analyte. The sample is deposited between the control and detection zones. A buffer release zone is located upstream of the conjugate pad and provides for buffer addition to the device, the buffer serving to move the detection probes to the detection and control zones.

The invention relates to a full-automatic fluorescent quantitative immunity analyzer and a method, and belongs to the technical field of detection. The full-automatic fluorescent quantitative immunity analyzer comprises a sample feeding device, a hatching device, a sampling device, a data acquisition device, a reagent card device and a system control module; the purpose of automatic quantitative immunity analysis is achieved through organic combination of all the devices and modules and mutual cooperation of all elements. According to the fluorescent quantitative immunity detection method, the full-automatic fluorescent quantitative immunity analyzer is adopted, therefore, full-automatic instrumental analysis is achieved, the detection efficiency is improved, and the professional requirement on an operator is lowered.

The invention discloses an application of nano-gold directly bonded with luminol in an immunoassay. The invention is characterized in that the immunoassay probe of a nano-gold directly bonded with luminol comprises antibodies which are labeled by the nano-golds directly bonded with luminol, wherein the nano-gold directly bonded with luminol are prepared through reducing chloroauric acid by the luminol at a single step; a chemiluminescence immunoassay method is based on the immunoassay probe of the nano-gold directly bonded with luminol; and a kit is used for carrying out the immunoassay method. The chemiluminescence immunoassay method of the invention has the advantages of high sensitivity (for instance, the detection limit can reach 1.0pg / mL for detecting human IgG), wide range of linearity, good repeatability, simple operation, low cost and the like, can be applied to detect multi-analyte in various samples and has key application prospect in fields, such as clinical diagnosis and cure, drug analysis, food security detection, environmental monitoring and the like.

It is an object of the present invention to provide magnetic substance-encapsulated particles which have uniform magnetism, high dispersion stability and a narrow particle size distribution, a method of producing the same, particles for immunoassay formed by using the magnetic substance-encapsulated particles and a method of immunoassay in which the magnetic substance-encapsulated particles or the particles for immunoassay are used. The present invention relates to a magnetic substance-encapsulated particle, which comprises an organic polymer material and a magnetic substance having an average particle size of 1 to 30 nm, the magnetic substance being contained within a particle in a state of being dispersed.

Immunoassay analytical test apparatus for allergy diagnosis, which apparatus comprises a zone for receiving a sample containing an analyte, a zone for receiving a mobile phase (the zone may be the same as the sample receiving zone, or different thereto), a detection means for permitting detection of the analyte by immunoreaction, a first flow path for flow of the analyte in the mobile phase from the sample receiving zone to the detection means, and a second flow path permitting flow of a mobile phase to the detection means.

The invention relates to the immunoassay medical field, particularly provides a magnetic particle competition method chemiluminescence immunoassay assay kit and a preparation method thereof used for detecting hormones. The kit according to the invention comprises: 1) a calibrator; 2) magnetic particles which are coated with streptavidin; 3) hormone antigens of enzyme markers; and 4) a chemiluminescence substrate. Further, the method for preparing the kit according to the invention has the following steps: 1) pure raw materials are used to prepare the calibrator; 2) the antigens are used to coat the magnetic particles; 3) the antigens of the enzyme markers are prepared; 4) the calibrator, the chemiluminescence substrate and the antigens of the enzyme markers are packaged in a separated way; and 5) a finished product is packaged. The kit has the advantages of convenience, rapidness, sensitivity, stability, and the like.

The invention relates to an immunoassay detection kit, and provides an allergenic specific IgE antibody immunoassay detection kit and a preparation method thereof. The detection kit comprises magnetic microspheres coupled with allergenic protein, magnetic microspheres coupled with anti-human IgE antibody, a human IgE antibody standard substance, alkali phosphatase labeled anti-human IgE antibody and an alkali phosphatase chemical luminous substrate AMPPD. The preparation method for the detection kit comprises the following steps: coupling the allergenic protein to the magnetic microspheres, coupling the anti-human IgE antibody to the magnetic microspheres, preparing the human IgE antibody standard substance, preparing the alkali phosphatase labeled anti-human IgE antibody, preparing the alkali phosphatase chemical luminous substrate AMPPD and packing the combination. The detection kit has the advantages of high sensitivity, high accuracy, quick response, convenient usage, low dosage and low cost, can assist in precisely and quantitatively measuring sensitive allergene, and guides clinical anaphylactic reaction treatment.

The invention discloses a multi-fluorescence immunity analysis method for quickly distinguishing PEDV, TGEV and PoRV. The method is easy to operate, a target amplified fragment is obtained through PCR, then hybridization is conducted on an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin, the MFi value is read through a detection instrument, and different types of viruses are distinguished. By means of the method, porcine epizootic diarrhea, swine transmissible gastroenteritis and pig group A rotavirus can be accurately detected at the same time, the specificity is high, the sensitivity is high, and the repeatability is good. Compared with a traditional detection method, various molecules of different purposes in the same sample are detected at the same time, the sample consumption is little, operation is simple and fast, and the detection cost can be greatly lowered.

Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.

The invention relates to a preparation method of a surface-functionalized molecularly imprinted polymer microsphere. The crosslinking degree of the molecularly imprinted polymer microsphere is above 60 percent, and therefore, the molecularly imprinted polymer microsphere has excellent molecule recognition to a template molecule and the particle size of 1-5 microns and is provided with an active function group on the surface. The molecularly imprinted polymer microsphere is prepared by adopting a new method of controllable / active free radical deposition and polymerization. The invention has the advantages of simple method, wide application range, pure product and the like. The prepared molecularly imprinted polymer microsphere has a wide application potential in mass fields of chromatographic stationary phase, biological sample analysis, medial clinic immunity analysis, food and environment monitoring, analogy enzyme catalysis, biomimetic sensors and the like.

The invention relates to an electrochemical immunosensor, a preparation method and application thereof, in particular to a sandwich type electrochemical immunosensor constructed by a marker based on dumbbell type Pt-Fe3O4 nano particles serving as a second antibody, a preparation method thereof, and application of the electrochemical immunosensor prepared by using the method in measurement of tumor markers. The electrochemical immunosensor is high in accuracy, stability, reproducibility and sensitivity, can quickly and conveniently perform immunoassay detection and can be used for clinical analysis.

The invention discloses a sealing and stabilizing agent for a microporous board. In terms of 100ml water, 0.1-3g of protein, 3-20g of saccharide, 0.01-1g of organic polymer, 0.01-0.05ml of nonionic surfactant, 0.01-0.1g of preservative and 10-100mmol of buffer solution are contained. The sealing and stabilizing agent disclosed by the invention is researched and designed on account of the characteristic that antigen or antibodies coated on a stationary phase are easy to inactivate. The sealing and stabilizing agent includes not only sealing components but also organic components and inorganic components, wherein the sealing components can be used for effectively sealing excessive loci on a sealing board, and the organic components and the inorganic component can be used for effectively stabilizing the antigen or the antibodies, so that the sealing purpose and the stabilizing purpose can be organically combined together through one-step operation. The sealing and stabilizing agent disclosed by the invention can be applied to immunological detection methods such as enzymelinked immunosorbent assay, chemiluminesent immunoassay, time resolved fluoroimmunoassay and the like, wherein the microporous board serves as the stationary phase; and the sealing and stabilizing agent can be used for effectively stabilizing the activity of the antigen or the antibodies coated on a stabilizing board.

The invention relates to a polymer chemiluminescent marker, and in particular relates to a polymer chemiluminescent labeling reagent as well as a preparation method and the application of the reagent. The polymer chemiluminescent labeling reagent comprises the following raw materials: a chemiluminescent marker, a polymer framework, and a ligand, a receptor or a functional group. The preparation method of the polymer chemiluminescent labeling reagent comprises the steps of (1) modifying the polymer framework; (2) modifying the ligand or the receptor; (3) synthesizing a ligand-polymer conjugate body or a receptor-polymer conjugate body; and (4) synthesizing the polymer chemiluminescent labeling reagent. The polymer chemiluminescent labeling reagent can be widely applied to the fields of medical diagnosis, biotechnology, biological medicine, life science, food safety, environmental detection, etc. Specifically, the polymer chemiluminescent labeling reagent is most commonly applied to immune analysis diagnosis, secondly applied to the analysis of the interaction between the ligand and the receptor in new drug research and development, and thirdly applied to nucleic acid or gene detection.

The invention discloses a luminol-luminescent functionalized nano-silver as well as a preparation method and an application for the same. The invention discloses a functionalized nano-silver, formed by connecting silver nanoparticles with luminol, wherein the luminol is connected to the surfaces of the silver nanoparticles via a covalent bond Ag-N. In the preparation method, nano-silver is prepared by directly reducing silver nitrate by luminol in the presence of ethanol, and luminol is used as a reducing agent and a protecting reagent simultaneously during synthesis process. The method has the advantages of being simple, fast, cheap and the like, as well as is a new technology for synthesising nano-silver at a temperature ranging from 25 to 40 DEG C. The particle size and shape of the nano-silver synthesised by the method can be regulated and controlled by the ratio of silver nitrate to luminol, and the obtained nano-silver has excellent chemiluminescent characteristic. The invention further discloses a bioanalysis probe based on the luminescent functionalized nano-silver, as well as an analysis method based on the probe and a kit. The functionalized nano-silver, the bioanalysis probe thereof, the analysis method and the kit disclosed by the invention can be used in the fields of immunoassay, nucleic acid analysis, molecular imaging, sensor etc.

The invention discloses a hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and a preparation and application thereof. In the mimic enzyme, nano-particles are of a hollow structure, the Pt / Cu / Co molar ratio in alloy composition is 1.33:1:1, and the average particle size is 32.2+ / -5.1 nm. The preparation method includes the steps that aqueous solutions of potassium chloroplatinate, copper chloride and nickel chloride are mixed, then polyvinylpyrrolidone, glycine and Co3O4 are added, and stirring and ultrasonic treatment are carried out to make the powder fully dispersed; thetemperature is increased to 180-210 DEG C, a constant-temperature reaction is carried out for 6-10 hours, after the reaction is finished, centrifugal separation and washing are carried out to obtainthe alloy nanoparticles, and the alloy nanoparticles are dispersed into water to obtain a platinum-copper-cobalt alloy nanoparticle mimic enzyme solution. The obtained platinum-copper-cobalt alloy nanoparticle mimic enzyme has very high chemical stability under strong acid, strong base, high salinity and high temperature conditions, and has potential application value in the fields of immunoassay,biological detection, clinical diagnosis and the like as a novel mimic enzyme.

An assay device for testing of liquid samples for drugs of abuse has a transparent container for retaining a liquid sample. A backing member is within the container and is curved so that its front surface corresponds to the curvature of the container wall. Immunoassay test strips are on the front face of the backing and are visible through the container wall. Each test strip is enclosed in a transparent pocket which has a bottom opening through which the bottom portion of the test strip protrudes to contact the liquid sample within the container. The liquid then flows upwardly through the test strip to react with reagents within the test strip.

The invention relates to a preparation method and application of a sandwich type immunosensor for detecting a stomach cancer tumor marker, and belongs to the field of novel nano functional materials,immunoassay and biological sensors. Three-dimensional porous molybdenum disulfide loading gold nano particle h-MoS2 / Au NPs is prepared to serve as a substrate material to modify a bare glassy carbon electrode, the solid supporting amount of the tumor marker to capture antibody is improved, and the electron transferring rate of electrode surface is effectively accelerated; an octahedral cuprous oxide / titanium dioxide core-shell structure loading mesoporous platinoid nano particle Cu2O@TiO2 / PtCu composite nano material is prepared to immobilize and detect the antibody, and the simple, rapid andsensitive sandwich type electrochemical immunosensor is prepared by utilizing the excellent electrochemical catalytic property of the composite nano material to hydrogen peroxide H2O2, and the immunosensor is used for detecting related tumor markers of stomach cancer.

The micro-amp immunity sensor based on mixed self-assembly film comprises: a silicon substrate, a SiO2 and Si3N4 insulation layer, and the concentric circular gold film electrode, SU-8 photoresist ring sensitive film pond, a ring Pt-film counter electrode / reference electrode, a Su-8 photoresist ring reaction pond, and a bio-molecular sensitive film fixed on the electrode. With nano technology and mixed self-assembly film, this invention has well consistence, correlativity and compatibility with IC, and shows a new path for immunity analysis and detection.

Systems and methods that analyze blood-based cancer diagnostic tests using multiple classes of molecules are described. The system uses machine learning (ML) to analyze multiple analytes, for example cell-free DNA, cell-free microRNA, and circulating proteins, from a biological sample. The system can use multiple assays, e.g., whole-genome sequencing, whole-genome bisulfite sequencing or EM-seq, small-RNA sequencing, and quantitative immunoassay. This can increase the sensitivity and specificity of diagnostics by exploiting independent information between signals. During operation, the system receives a biological sample, and separates a plurality of molecule classes from the sample. For a plurality of assays, the system identifies feature sets to input to a machine learning model. The system performs an assay on each molecule class and forms a feature vector from the measured values. The system inputs the feature vector into the machine learning model and obtains an output classification of whether the sample has a specified property.

The invention relates to a pathogen detection method based on a micro-fluidic chip. The method adopts a micro-fluidic chip integrated with a micro-magnetic field as a reaction container, adopts magnetic balls as solid phase carriers, and adopts quantum dots modified by streptavidin (SA-QDs) as fluorescent markers; under the action of the micro-magnetic field, magnetic balls are captured at specific parts in a chip channel, and thus a micro-reaction zone is formed; pathogens are captured through a sandwich immunization reaction; with the interaction between biotin and streptavidin, the immunofluorescence quantitative analysis of the pathogens is realized. The detection method combines the micro-fluidic chip, magnetic immunoassay, and fluorescence detection, has the advantages of rapidness, high efficiency, and few sample using amount for the micro-fluidic chip, high specificity and strong manoeuvrability for magnetic immunoassay, and excellent optical properties for quantum dots, and is a multi-objective real-time pathogen detection method.

The invention belongs to the technical fields of immunoassay and biosensing and discloses a preparation method and application of an electrochemical immunosensor based on an HS-beta-CD-Ag-GOD conjugate. The electrochemical immunosensor is used for rapidly detecting a hog cholera virus antigen CSFV. The manufacturing scheme comprises the following steps: modifying a working electrode a bare platinum carbon electrode by using MWCNTs-CD-Fc-Ab1, and sequentially adding bovine serum albumin, hog cholera virus antigen CSFV and an Ab2-HS-beta-CD-Ag-GOD conjugate. The HS-beta-CD-Ag-GOD conjugate can convert glucose into gluconic acid, two protons and two electrons are transferred to oxygen molecules, hydrogen peroxide is generated, and an HS-beta-CD-Ag nanometer material serves as a mimic enzyme, the reduction of the hydrogen peroxide is catalyzed, the dual amplification of an electrochemical signal is realized, the high sensitivity is realized, and the detection limit can be low to 0.33pg / mL.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for phosphatidylinositol proteoglycan-3(GPC-3) and a preparation method thereof, and realizes the simultaneous serological detection of GPC-3 N terminal and C-terminal protein with the chemiluminescent immunoassay method. The kit has the advantages of simple sampling, convenient detection and accurate and specific technical method. The invention adopts a biotin-strapavidin system to coat antibodies and improve the efficiency of antibody coating and the linear range of detection as well as sensitivity, and can be conveniently used for the tracing observation of early diagnosis or treatment effect for primary carcinoma of liver.

This invention discloses a citrinin immune chromatography test paper and its process method and utility. The test paper comprises pad, sample pad, tracing particle compound pad, pyroxylin film, adsorption pad, test thread, and control thread. The method is the following: to orderly put sample pad, tracing particle compound pad, pyroxylin film and adsorption pad on the pad; the tracing particle compound pad has citrinin antibody with tracing particle mark; the pyroxylin film has test thread and control thread; the test thread has citrinin antigen and antibody of citrinin antigen.

The invention discloses a double-antibody sandwich method for detecting salmonella typhimurium in food based on monoclonal antibodies, belonging to the technical field of immunoassay. Salmonella typhimurium ATCC13311 and smooth salmonella typhimurium LPS are adopted for mixed immunity of a 7-week BALB / c mouse, 10 LPS monoclonal antibodies are obtained by immunity, fusion and screening, horse radish peroxidases (HRP) are labeled respectively, and the salmonella typhimurium is paired two by two. A sandwich enzyme-linked immuno sorbent assay (ELISA) method is established by taking 6E2 CGMCC No.7206 monoclonal antibodies as coated antibodies and enzyme-labeled antibodies and by taking the salmonella typhimurium as standards, and the LOD is 500cfu / mL. The sandwich method, established by using the monoclonal antibodies which are highly uniform in physicochemical property and high in specificity and can be prepared on a large scale, is high in sensitivity and low in cost; the salmonella typhimurium is not in cross reaction with salmonella enteritidis, salmonella arizonae, E.coli, E.coliO157:H7, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes; a quick and efficient analysis way is provided for detection of the salmonella typhimurium in the food.

The invention belongs to the technical field of biological analysis and detection, in particular to a microfludic chip for rapid detection of microcystins and a preparation method thereof. The microfludic chip is made of optically transparent polydimethylsiloxane and the like by a molding method, and mainly comprises a sample reaction microchannel layer, a valve control layer and a substrate layer. The microfludic chip comprises a sample enriched and immunoassay module which consists of parallel immune chromatographic column microanalysis chambers in nanolitre volume. Microcystin antibody proteins or antigens are fixed in each analysis chamber, and rapid on-site detection of representative algal toxins, namely microcystins in samples with different sources is realized. Besides the advantages of high sensitivity and specificity, the microfludic chip has the characteristics of rapidness, high efficiency, portability, low cost and easily automatic control, can finish automatic signal acquisition, remote transmission and signal analysis, and is suitable for on-site rapid detection and remote control detection in a wide range for the microcystins in the water environment.

The invention relates to a method for detecting microcystin-LR under condition that the end surface of a gold nanorod is self-assembled and mediated by using a Raman spectrum, belonging to the technical field of immunoassay chemistry. In the invention, the end surface of the gold nanorod is respectively coupled with a coating source of the microcystin-LR and an antibody for resisting the microcystin-LR to form a probe of the gold nanorod, and the synthesized gold nanorod probe is utilized to carry out an immune reaction so as to form nano chains due to an antigen antibody reaction on the end surface of the gold nanorod; the difference of the lengths of assembled nano chains ensures that the intensities of Raman signals are different; and the purpose of detecting the content of the MC-LR is achieved through the changes of the Raman signals. The reaction in the invention is carried out in a liquid environment without a cleaning step, and only one reaction step is needed, thereby reaction conditions are simplified, the labor intensity is lightened, and the detection sensitivity is improved.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for free prostate specific antigens and a preparation method thereof. The kit of the invention comprises: 1) free prostate specific antigen calibrators, 2) solid-phase vectors which are coated by prostate specific antigen monoclonal antibodies, 3) anti-free prostate specific antigen antibodies which are marked by enzyme and 4) chemiluminescent substrate solutions. Further, the preparation method of the kit according to the invention comprises the following steps: 1) preparing the calibrators, 2) coating the solid-phase vectors with the free prostate specific antigen monoclonal antibodies, 3) marking the anti-free prostate specific antigen antibodies with the enzyme, 4) packaging the calibrators, the enzyme markers and chemiluminescent substrate solutions, and 5) assembling finished products. The kit of the invention has the advantages of convenience, rapidness, sensitivity, stability, and the like.