71 results about "Fluorescence immunoassay" patented technology

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention relates to the technical field of fluorescence immunoassay, and discloses a quantum resonance fluorescence immunoassay medical detection method, which comprises the following steps: 1, carrying out fixation and permeation treatment on cells, respectively incubating the cells by using labeled different quantum dot-antibody compounds, specifically binding antibodies with corresponding target proteins in the cells, and detecting the target proteins in the cells; a labeled cell sample is obtained; 2, under a confocal microscope, laser with a specific wavelength is adopted to excite the marked cell sample, and fluorescence lifetime image data of different quantum dot emission wavelengths are collected; according to the method, the fluorescence lifetime image data of different quantum dot emission wavelengths are acquired through the confocal microscope, the position and content information of different target proteins can be clearly distinguished, simultaneous analysis and accurate positioning of multiple target proteins are realized, and an efficient means is provided for multi-protein research in a complex biological system.

The invention belongs to the technical field of immunoassay rapid detection, and relates to a method for screening residues of traditional Chinese medicinal material aflatoxin B1 based on multicolor evolution fluorescence immunochromatography, which comprises the following steps: coupling red quantum dot fluorescent microspheres with an AFB1 antibody to prepare a detection probe; coupling or mixing the green quantum dot fluorescent microspheres with an AFB1 antigen to prepare an internal standard probe; respectively coating an internal standard probe and goat anti-mouse IgG on an NC membrane to complete preparation of a T line and a C line; preparing a test solution containing a detection probe, dripping the test solution on a test paper sample pad, and observing fluorescence colors of a T line and a C line by ultraviolet; and judging whether the residual quantity of the traditional Chinese medicine AFB1 exceeds the limit specification or not according to the T-line fluorescence color. By introducing the internal standard probe, a ratio fluorescence immunoassay system is constructed, so that the sensitivity and the accuracy of an analysis result can be improved, and the interference of a sample matrix is reduced; the used detection test paper is small in matrix influence and wide in application range, and can be used for rapid limited screening of AFB1 residues in traditional Chinese medicinal materials.

The invention provides a quadruple test method for inflammatory factor markers CRP, SAA, PCT and IL-6, and belongs to the technical field of biological detection. According to the present invention, the digital fluorescence immunoassay technology is adopted, the antibodies are immobilized on the detection plate, and after the antigen in the sample is combined with the immobilized antibodies, the fluorescein-labeled antibody is combined with the other antigen determinant on the antigen so as to detect the antigen in the sample; therefore, a sandwich type compound structure is constructed between the immobilized antibody and the fluorescein labeled antibody, the higher the antigen concentration is, the more the fluorescent particles are, and the content of the antigen is quantified through the number of the fluorescent particles. By means of the unique design, high-sensitivity and high-precision detection of antigens can be achieved, the content of the antigens can be accurately quantified by accurately counting the number of the fluorescent particles, the influence of lipemia / hemolysis on the detection result can be avoided, the detection range is wide, sensitivity is high, accuracy is high, and detection is automatic, convenient and rapid.

The invention relates to the technical field of fluorescence immunoassay, in particular to an immunoassay instrument. The immunity analyzer comprises: a detection module suitable for inserting a plurality of test pieces; a scanning unit, a light path unit and a camera shooting unit are arranged in the detection module; the scanning unit is used for acquiring information parameters of the test pieces, the light path unit is used for exciting light to enable all the test pieces to generate light spots, and the camera shooting unit is used for acquiring image information of the light spots; the shell module is in communication connection with the detection module; the shell module is used for dividing the image information into an edge area and a middle area and adjusting the edge area; and calculating the concentration value of the to-be-tested object on the test piece based on the middle area. According to the embodiment, the edge and the middle area can be accurately distinguished, and interference of edge brightness on the middle area is avoided. Meanwhile, only the marginal area is processed, the detection result of other parts is not influenced, and the accuracy of a fluorescence signal is ensured.

The invention discloses a brain-derived Tau protein single-molecule fluorescence immunoassay detection kit and a use method, the kit provided by the invention is composed of a BD-Tau magnetic bead reagent coated with a capture antibody, a BD-Tau calibrator, a BD-Tau quality control product, a biotinylated BD-Tau Detector reagent for detecting the antibody, a BD-Tau SBG reagent, an RGP reagent and a BD-Tau sample diluent, the magnetic bead concentration of the BD-Tau magnetic bead reagent is 0.04 mg / mL-0. 08mg / mL of carboxyl magnetic beads, the carboxyl magnetic beads are coated with Tau J5.H3 coated antibodies, the detection antibody in the BD-Tau Detector reagent of the biotinylated detection antibody is Tau12, the concentration of the detection antibody is 0.3 mu g / mL-0. 8mu g / mL, and the concentration of SBG in the BD-Tau SBG reagent is 40 pM-60 pM; the kit disclosed by the invention can be used by adopting any one of a one-step method, a two-step method or a three-step method to detect the content of the BD-Tau protein in a plasma sample, the detection limit is 0.050 pg / mL, and the detection range is 0.050 pg / mL-150 pg / mL; according to the invention, the specificity, accuracy and reliability of determination of the Alzheimer's disease are improved, and the problems of early diagnosis and early screening of the Alzheimer's disease are solved.

The present invention discloses a preparation method and application of a composite probe for gold nanoclusters emitting enhanced fluorescence induced by the spatial confinement effect of ZIF-8 surface. HAuCl4 and glutathione are dissolved in water, the two solutions are mixed and reacted, and purified to obtain an AuNCs solution; Zn(NO3)2·6H2O and 2-methylimidazole are dissolved in methanol, the two solutions are mixed and reacted, and the generated white precipitate is treated to obtain a zeolite imidazole framework-8 solution; the obtained AuNCs solution and the zeolite imidazole framework-8 solution are mixed, stirred, self-assembled, and treated to obtain ZIF-8@(Au) nanoparticles; zinc nitrate and dimethylimidazole are added to the nanoparticle solution for reaction, and after treatment, a sandwich ZIF-8@Au@ZIF-8 nanomaterial is obtained; the nanomaterial is mixed and treated with the AuNCs solution to obtain a ZIF-8@Au loaded with two layers of AuNCs; the operation is repeated to obtain a ZIF-8@(Au@ZIF-8) loaded with n layers of AuNCs. n The present invention significantly improves the effective utilization rate of assembly space and fluorescence performance, and realizes ultra-sensitive detection of fluorescence immunoassay.

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

The utility model discloses a reagent box for fluorescence immunoassay, which belongs to the technical field of biochemical detection.The reagent box for fluorescence immunoassay comprises a box body and a box door, and the box door is hinged to the box body; a plurality of ice cavities are formed in the pulling plate, and the ice cavities are filled with ice bags; wherein a gap is formed between every two ice cavities, the gaps are used for containing reagents, the reagents are attached to the outer side walls of the ice cavities, and the pulling plate freely slides in the box body and can stretch out of the box body; wherein the ice cavity is higher than the reagent; wherein the drawing plate is a structural member made of a low heat conduction coefficient, and the ice cavity is a structural member made of a high heat conduction coefficient material. According to the utility model, the technical problem that the transportation cost of the fluorescence immunoassay reagent is too high in the short-distance transportation process can be solved.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The utility model provides an automatic sampling device for a fluorescence immunoassay analyzer, which comprises a fixed bracket arranged on the fluorescence immunoassay analyzer, the X-axis movement mechanism is connected with the fixed bracket in a sliding manner; the Y-axis movement mechanism is mounted on the X-axis movement mechanism; the sampling arm assembly is positioned in the Z-axis direction and is fixedly connected with the X-axis movement mechanism; the X-axis movement mechanism is fixedly connected with the sampling arm assembly through an X-axis protection chain support, and the Y-axis movement mechanism is fixedly connected with the Y-axis protection chain support through a Y-axis protection chain support. According to the utility model, on the basis of ensuring that the sampling arm assembly effectively completes automatic sampling and moving processes in the operations of cleaning, washing liquid, spitting liquid and uniformly mixing, the tension of the wire harness on the bent parts of the X-axis protection chain and the Y-axis protection chain is reduced through the X-axis protection chain bracket and the Y-axis protection chain bracket, so that the protection chains are prevented from being damaged and broken; the service life of the automatic sampling device is prolonged, and the normal detection of a fluorescence immunoassay instrument is ensured.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a magnetic bead bearing plate sealing imaging mechanism which comprises a mounting bottom plate, a bearing mechanism and an imaging mechanism, the bearing mechanism comprises a bearing disc arranged on the mounting bottom plate, a bearing groove formed in the bearing disc, a shaft hole formed in the center of the bearing groove, a rotating shaft arranged in the shaft hole and a rotating motor arranged at the lower end of the bearing disc, the upper end of the rotating shaft penetrates through the shaft hole and extends upwards, and the lower end of the rotating shaft is in transmission connection with the rotating motor; the imaging mechanism comprises a supporting frame arranged at the lower end of the mounting bottom plate and a fluorescence microscope arranged on the supporting frame, an observation hole is formed in the bearing groove, and the lens end of the fluorescence microscope corresponds to the observation hole. The design is simple in structure, low in cost and stable in operation.

The utility model relates to the technical field of quantum dot fluorescence immunoassay analyzers, in particular to a detection platform for a quantum dot fluorescence immunoassay analyzer. The detection platform for the quantum dot fluorescence immunoassay analyzer comprises a detection table and an immunoassay analyzer body mounted on one side of the detection table, and the immunoassay analyzer body is provided with a detection window for placing a sample for detection; the control box is mounted on the other side of the detection table; the stand column is fixedly installed on the detection table and located on one side of the detection window, a sleeve is rotationally installed on the stand column, a hollow annular disc is fixedly installed at the top end of the sleeve, a plurality of through grooves are evenly formed in the annular disc, supporting shafts are rotationally installed in the through grooves through damping sleeve shafts, and bearing blocks are fixedly installed on the supporting shafts; the upper end and the lower end of the bearing block are each provided with a containing assembly used for containing a sample. The detection platform for the quantum dot fluorescence immunoassay analyzer provided by the utility model has the advantage of high efficiency and rapidness in detection.

The invention relates to the technical field of detection equipment, and discloses a biochemical blood coagulation and immunity integrated analyzer which comprises a shell, a biochemical blood coagulation detection module and an immunity detection module which are arranged in the shell, a biochemical blood coagulation detection port and an immunity detection port which are formed in the front surface of the shell, and a baffle plate arranged at an opening of the biochemical blood coagulation detection port, the heat dissipation channel is formed in the side face of the shell, and the biochemical blood coagulation detection module extends out of the immunodetection module. According to the biochemical blood coagulation and immunity integrated analyzer, the biochemical blood coagulation detection module and the immunity detection module are integrated in the shell, multiple devices do not need to detect for several times, integrated detection of biochemical, blood coagulation and immune markers is achieved, the detection efficiency is greatly improved, and the medical cost is reduced; based on the advantages of portability of microfluidics and high sensitivity of fluorescence immunoassay, POCT scene requirements are met, the sample dosage and detection time consumption are reduced, and support is provided for rapid clinical diagnosis.

The present application provides an optical path transmission mechanism and an optical circuit module of a dry fluorescent immunoassay analyzer, wherein the optical path transmission mechanism comprises a light source (5), and further comprises: a first convex mirror (11), arranged at a first relative position of the light source (5), so that the light emitted by the light source (5) can be transmitted to a reagent strip reactant through the first convex mirror (11); a second convex mirror (9), arranged at a second relative position of the first convex mirror (11), so that the reflected light of the reagent strip reactant can be transmitted to the second convex mirror (9) through the first convex mirror (11), and then transmitted out through the second convex mirror (9). In the technical solution provided by the present application, the intensity of the optical signal is improved, and the uncertainty of the measurement result of the dry fluorescent immunoassay analyzer is reduced.

The invention relates to the field of fluorescence immunoassay equipment, and provides full-automatic fluorescence immunoassay equipment which comprises a card bin base, a reagent card bin and a card pushing assembly, the card bin base is provided with a card slot, and the bottom of the card slot is provided with a first through slot. The reagent card bin is placed in the clamping groove, the reagent card bin comprises a box body and reagent cards, the reagent cards are placed in the box body, and the box body is provided with a pushing notch and a discharging port; the card pushing assembly comprises a card pushing unit, an X-direction driving unit and a Z-direction driving unit, the X-direction driving unit and the Z-direction driving unit are both connected with the card pushing unit, the Z-direction driving unit is used for driving the card pushing unit to ascend and descend, and the X-direction driving unit is used for driving the card pushing assembly to push out the first reagent card; the card pushing unit comprises a mounting block, a card pushing piece and a partition plate; the card pushing piece is used for pushing the reagent card to move; the partition plate is located above the card pushing piece and used for being inserted into the lower portion of the second reagent card. The problems that the reagent card supply efficiency is low, and the jamming phenomenon is prone to occurring are solved.

The utility model provides a fluorescence immunoassay analyzer with a suction head tray positioning structure, and relates to the technical field of fluorescence immunoassay analyzers, the fluorescence immunoassay analyzer comprises a base assembly and a positioning detection mechanism, a clamping transfer part assembled by a bolt is arranged above one side of the base assembly, and the positioning detection mechanism is arranged above the other side of the base assembly. A positioning detection mechanism for bolt assembly is arranged on the top side of the base assembly, and the positioning detection mechanism comprises a sleeving base, a side shell, a spring rod set, a clamping piece, a limiting block, a lower sleeving rod, an inserting plate, a magnetic suction cup, a connecting pipe, an air pump and a discharging opening; the magnetic suction cup is arranged below the lower sleeve rod, the lower sleeve rod is connected to the side shell in an inserted mode, the inserting plate is fixed through cooperation of the spring rod set, the clamping piece and the limiting block, and the magnetic suction cup and the lower sleeve rod are effectively connected after a product is fixed. And finally, through output power of an air pump, a magnetic suction cup can effectively suck the lower sleeve rod tightly, so that the suction tightness degree of the position can be improved, and the position accuracy is guaranteed.

The present invention provides a temperature-corrected analyte detection method, which involves adding a series of analyte calibrators with different concentrations to an immunoassay strip, and then using an optical analyzer to measure the signal values generated after each concentration of analyte calibrator is captured on the detection pad of the immunoassay strip at each segmented temperature point. Then, with the T / C value at each segmented temperature point as the X-axis and the concentration of the analyte calibrator as the Y-axis, a corresponding equation is plotted; then, according to the selected n segmented temperature points, temperature segmentation is performed, and different formulas are used for calculation in different temperature segments. This temperature-corrected analyte detection method can be based on time-resolved fluorescence immunoassay and is used to more accurately detect myocardial markers, upper respiratory virus infections, metabolic hormones, digestive tract series markers, allergens, etc.

The invention belongs to the technical field of analysis and detection, and particularly discloses a capillary electrophoresis fluorescence immunoassay method for detecting abrus precatorius toxin. A fluorescein isothiocyanate (FITC) labeled abrus precatorius toxin antibody probe is prepared, a non-competitive mode is adopted, a sample solution and an FITC labeled antibody are subjected to an incubation reaction, and the FITC labeled antibody is relatively excessive. The FITC labeled antibody-antigen compound and the excessive FITC labeled antibody in the mixed solution are divided into different zones in the separation capillary tube according to different migration rates, and fluorescence signal detection is performed through a window of a laser-induced fluorescence detector in sequence. Qualitative and quantitative analysis is carried out on the abrus precatorius toxin through the fluorescence signal peak and the intensity of the compound. According to the present invention, the detection method of the abrus precatorius toxin fluorescence immunocapillary electrophoresis is clarified, the detection automation is improved while the detection time is reduced compared with the traditional immunization method, the linear range of the abrus precatorius toxin standard solution detection is 75.8-606.4 nM, and the detection limit is 75.8 nM.

The invention provides a preparation process of a Hep-2 cell extract suitable for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies and a preparation method of the test strip. The method comprises the following steps: transferring cultured Hep-2 cells into a PBS (Phosphate Buffer Solution), carrying out centrifugal separation on the obtained Hep-2 cell suspension to obtain Hep-2 cell agglomerates, carrying out ultrasonic treatment on a mixture of the Hep-2 cell agglomerates and the extraction buffer solution in an ice bath, centrifuging the mixture subjected to ultrasonic treatment, collecting supernatant, and passing the supernatant through a micro-filtration membrane to obtain filtrate, namely the Hep-2 cell extract. The extraction buffer solution is an HEPES buffer solution containing isopropanol, trehalose, bovine serum albumin and phenylmethylsulfonyl fluoride. The prepared HEP-2 cell extract is used for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies, and the detection sensitivity, the specificity and the reliability of a detection result can be considered at the same time.

The invention discloses a fluorescence immunoadsorption method for detecting an O-type foot-and-mouth disease virus VP1 protein antibody and application, and relates to the technical field of genetic engineering, and the method is characterized by comprising the following steps: S1, preparing O-type foot-and-mouth disease virus VP1 protein by using a prokaryotic expression system; s2, preparing an anti-O type foot-and-mouth disease VP1 protein monoclonal antibody capable of recognizing a VP1 protein epitope; s3, coupling quantum dots with the anti-O-type foot-and-mouth disease VP1 protein monoclonal antibody to obtain a novel fluorescent probe; and S4, quantitatively detecting the O-type foot-and-mouth disease virus antibody through a fluorescence immunoadsorption method. According to the method, quantum dots and an O-type foot-and-mouth disease VP1 protein resisting monoclonal antibody are coupled to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of the O-type foot-and-mouth disease virus antibody, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay, the method is more sensitive.

This invention discloses a fluorescence immunoassay analyzer, belonging to the technical field of fluorescence immunoassay devices, designed to solve the problem of continuous sample injection in existing devices. The fluorescence immunoassay analyzer disclosed in this invention includes: a housing; a rotating sample holder including a rotating stage with at least two sample slots for holding sample tubes, the sample tubes rotating synchronously with the rotating stage; a vortex oscillation mechanism for vortexing and oscillating the sample tubes; a testing mechanism; a transport mechanism for transporting the sample tubes between the rotating sample holder and the vortex oscillation mechanism; and a pipetting mechanism for adding or extracting liquid from the sample tubes. The fluorescence immunoassay analyzer disclosed in this invention includes a rotating stage, allowing the next batch or more batches of sample tubes to be placed on the rotating stage and injected with extraction liquid while the previous batch of sample tubes is vortexed on the vortex oscillation mechanism, achieving continuous sample injection and high detection efficiency.

The invention discloses a method for detecting clothianidin and thiamethoxam in ginger based on quantum dot microsphere fluorescence immunochromatography, and belongs to the technical field of compound detection. The method comprises the following steps: (a) extracting a ginger sample by adopting an acetonitrile solution, and diluting supernate by using a PBS (Phosphate Buffer Solution) to obtain a solution to be detected; (b) dropwise adding a solution to be detected onto the sample pad of the test strip, incubating, reading a fluorescence signal by using a quantum dot microsphere fluorescence immunoassay analyzer, respectively obtaining signal intensities and ratios of detection lines and quality control lines of clothianidin and thiamethoxam, correspondingly substituting the ratios into a clothianidin and thiamethoxam standard curve, and calculating to obtain the content of clothianidin and thiamethoxam. The method disclosed by the invention has the characteristics of uniform sample pretreatment, simplicity, convenience and rapidness in detection, high sensitivity, capability of simultaneously providing quantitative results of two items and the like, and is suitable for on-site screening of large-batch samples.

The immunochromatography analyzer comprises an optical module and a base, the base is provided with a placing groove for placing a reagent card, the placing groove extends along a first direction of the base, and the optical module is arranged above the placing groove; wherein the optical modules comprise at least one of a colloidal gold module for carrying out colloidal gold immunoassay detection and a fluorescent module for carrying out fluorescence immunoassay detection, and when the number of the optical modules is more than two, the colloidal gold module is used for carrying out colloidal gold immunoassay detection; and each optical module can be independently subjected to position adjustment in a second direction relative to the base, and an included angle is formed between the second direction and the first direction.

The invention relates to the technical field of nano biosensing, in particular to a mycoplasma bovis rapid detection method based on a nano antibody, which comprises the following steps: expressing a mycoplasma bovis specific nano antibody by gene-edited escherichia coli, and culturing in a culture medium containing a novel protein expression accelerant and a self-assembly inducer to obtain the mycoplasma bovis rapid detection method based on the nano antibody. Chemical marking is carried out through clicking by a special fluorescent marker; treating a sample with a surfactant and a nuclease inhibitor, centrifuging, taking supernatant, and reacting with the labeled nano antibody and the signal amplifying agent in the micro-fluidic chip; magnetic separation is combined with time-resolved fluorescence immunoassay, and a fluorescence signal ratio is detected through dual-wavelength excitation, so that whether the mycoplasma bovis exists in a sample is judged. The detection method is rapid in detection and can be completed within one hour; the accuracy is high, and misjudgment is avoided; the operation is simple and convenient; cost is low and popularization is easy; and high throughput and universality are achieved.

This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a reagent card positioning device for a fluorescence immunoassay analyzer. It includes an instrument assembly, a reagent card assembly, and a positioning assembly. The instrument assembly includes an analyzer body, the reagent card assembly includes a reagent card body, and the positioning assembly includes a linkage slide plate and a handle. A groove is formed on the inner side of the front end of the analyzer body, and a positioning inner post is formed on the inner side of the analyzer body. A reagent hole is provided on the inner side of the upper part of the reagent card body, and a positioning inner hole is formed on the inner side of the front wall of the reagent card body. One end of the linkage slide plate is connected to a positioning insertion rod. The analyzer body is designed for fluorescence immunoassay analysis of samples within the reagent card body. The elastic buffer pad absorbs the impact force when the reagent card body is inserted, preventing sample splashing or displacement due to collision within the reagent hole.

This application relates to the field of fluorescence immunoassay equipment, and provides a fully automatic fluorescence immunoassay device, which includes a cartridge base, a reagent cartridge, and a card pushing assembly; the cartridge base is provided with a card slot, and a first through groove is provided at the bottom of the card slot; the reagent cartridge is placed in the card slot, and the reagent cartridge includes a box body and a reagent card, the reagent card is placed in the box body, and the box body is provided with a material pushing notch and a discharge port; the card pushing assembly includes a card pushing unit, an X-direction driving unit, and a Z-direction driving unit, both the X-direction driving unit and the Z-direction driving unit are connected to the card pushing unit, the Z-direction driving unit is used to drive the card pushing unit to lift, and the X-direction driving unit is used to drive the card pushing assembly to push out the first reagent card; the card pushing unit includes a mounting block, a card pushing member, and a partition plate; the card pushing member is used to push the reagent card to move; the partition plate is located above the card pushing member and is used to be inserted under the second reagent card. This application solves the problems of low reagent card supply efficiency and easy jamming phenomenon.

The present invention constructs a method for a three-enzyme cascade-triggered fluorescence immunosensing platform by sequentially integrating ALP, TYR, and HRP. This platform relies on the fact that HRP and H2O2 can promote the rapid in-situ fluorescence reaction of dopamine and 1,5-naphthalenediol to produce a strong yellow fluorescent compound (AFC). The molecular formula (C 18 H 15 NO4) and structure of the synthesized AFC were determined by nuclear magnetic resonance mass spectrometry characterization, and based on the fact that ALP can induce the hydrolysis of p-aminophenylethyl phenyl phosphate (PAPP) to produce the intermediate tyramine, and TYR can catalyze the production of DA, which in turn activates HRP, a three-enzyme fluorescence immune system was constructed. The high-sensitivity detection of cardiac troponin I was achieved using this system. This advanced signal transduction scheme can provide a new method for studying multi-enzyme cascade systems and constructing multifunctional biosensors.