27 results about "Fluorescence immunoassay" patented technology

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a magnetic bead bearing plate sealing imaging mechanism which comprises a mounting bottom plate, a bearing mechanism and an imaging mechanism, the bearing mechanism comprises a bearing disc arranged on the mounting bottom plate, a bearing groove formed in the bearing disc, a shaft hole formed in the center of the bearing groove, a rotating shaft arranged in the shaft hole and a rotating motor arranged at the lower end of the bearing disc, the upper end of the rotating shaft penetrates through the shaft hole and extends upwards, and the lower end of the rotating shaft is in transmission connection with the rotating motor; the imaging mechanism comprises a supporting frame arranged at the lower end of the mounting bottom plate and a fluorescence microscope arranged on the supporting frame, an observation hole is formed in the bearing groove, and the lens end of the fluorescence microscope corresponds to the observation hole. The design is simple in structure, low in cost and stable in operation.

The utility model relates to the technical field of quantum dot fluorescence immunoassay analyzers, in particular to a detection platform for a quantum dot fluorescence immunoassay analyzer. The detection platform for the quantum dot fluorescence immunoassay analyzer comprises a detection table and an immunoassay analyzer body mounted on one side of the detection table, and the immunoassay analyzer body is provided with a detection window for placing a sample for detection; the control box is mounted on the other side of the detection table; the stand column is fixedly installed on the detection table and located on one side of the detection window, a sleeve is rotationally installed on the stand column, a hollow annular disc is fixedly installed at the top end of the sleeve, a plurality of through grooves are evenly formed in the annular disc, supporting shafts are rotationally installed in the through grooves through damping sleeve shafts, and bearing blocks are fixedly installed on the supporting shafts; the upper end and the lower end of the bearing block are each provided with a containing assembly used for containing a sample. The detection platform for the quantum dot fluorescence immunoassay analyzer provided by the utility model has the advantage of high efficiency and rapidness in detection.

The invention relates to the technical field of detection equipment, and discloses a biochemical blood coagulation and immunity integrated analyzer which comprises a shell, a biochemical blood coagulation detection module and an immunity detection module which are arranged in the shell, a biochemical blood coagulation detection port and an immunity detection port which are formed in the front surface of the shell, and a baffle plate arranged at an opening of the biochemical blood coagulation detection port, the heat dissipation channel is formed in the side face of the shell, and the biochemical blood coagulation detection module extends out of the immunodetection module. According to the biochemical blood coagulation and immunity integrated analyzer, the biochemical blood coagulation detection module and the immunity detection module are integrated in the shell, multiple devices do not need to detect for several times, integrated detection of biochemical, blood coagulation and immune markers is achieved, the detection efficiency is greatly improved, and the medical cost is reduced; based on the advantages of portability of microfluidics and high sensitivity of fluorescence immunoassay, POCT scene requirements are met, the sample dosage and detection time consumption are reduced, and support is provided for rapid clinical diagnosis.

The invention belongs to the technical field of analysis and detection, and particularly discloses a capillary electrophoresis fluorescence immunoassay method for detecting abrus precatorius toxin. A fluorescein isothiocyanate (FITC) labeled abrus precatorius toxin antibody probe is prepared, a non-competitive mode is adopted, a sample solution and an FITC labeled antibody are subjected to an incubation reaction, and the FITC labeled antibody is relatively excessive. The FITC labeled antibody-antigen compound and the excessive FITC labeled antibody in the mixed solution are divided into different zones in the separation capillary tube according to different migration rates, and fluorescence signal detection is performed through a window of a laser-induced fluorescence detector in sequence. Qualitative and quantitative analysis is carried out on the abrus precatorius toxin through the fluorescence signal peak and the intensity of the compound. According to the present invention, the detection method of the abrus precatorius toxin fluorescence immunocapillary electrophoresis is clarified, the detection automation is improved while the detection time is reduced compared with the traditional immunization method, the linear range of the abrus precatorius toxin standard solution detection is 75.8-606.4 nM, and the detection limit is 75.8 nM.

The invention discloses a fluorescence immunoadsorption method for detecting an O-type foot-and-mouth disease virus VP1 protein antibody and application, and relates to the technical field of genetic engineering, and the method is characterized by comprising the following steps: S1, preparing O-type foot-and-mouth disease virus VP1 protein by using a prokaryotic expression system; s2, preparing an anti-O type foot-and-mouth disease VP1 protein monoclonal antibody capable of recognizing a VP1 protein epitope; s3, coupling quantum dots with the anti-O-type foot-and-mouth disease VP1 protein monoclonal antibody to obtain a novel fluorescent probe; and S4, quantitatively detecting the O-type foot-and-mouth disease virus antibody through a fluorescence immunoadsorption method. According to the method, quantum dots and an O-type foot-and-mouth disease VP1 protein resisting monoclonal antibody are coupled to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of the O-type foot-and-mouth disease virus antibody, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay, the method is more sensitive.

This invention discloses a fluorescence immunoassay analyzer, belonging to the technical field of fluorescence immunoassay devices, designed to solve the problem of continuous sample injection in existing devices. The fluorescence immunoassay analyzer disclosed in this invention includes: a housing; a rotating sample holder including a rotating stage with at least two sample slots for holding sample tubes, the sample tubes rotating synchronously with the rotating stage; a vortex oscillation mechanism for vortexing and oscillating the sample tubes; a testing mechanism; a transport mechanism for transporting the sample tubes between the rotating sample holder and the vortex oscillation mechanism; and a pipetting mechanism for adding or extracting liquid from the sample tubes. The fluorescence immunoassay analyzer disclosed in this invention includes a rotating stage, allowing the next batch or more batches of sample tubes to be placed on the rotating stage and injected with extraction liquid while the previous batch of sample tubes is vortexed on the vortex oscillation mechanism, achieving continuous sample injection and high detection efficiency.

The invention discloses a method for detecting clothianidin and thiamethoxam in ginger based on quantum dot microsphere fluorescence immunochromatography, and belongs to the technical field of compound detection. The method comprises the following steps: (a) extracting a ginger sample by adopting an acetonitrile solution, and diluting supernate by using a PBS (Phosphate Buffer Solution) to obtain a solution to be detected; (b) dropwise adding a solution to be detected onto the sample pad of the test strip, incubating, reading a fluorescence signal by using a quantum dot microsphere fluorescence immunoassay analyzer, respectively obtaining signal intensities and ratios of detection lines and quality control lines of clothianidin and thiamethoxam, correspondingly substituting the ratios into a clothianidin and thiamethoxam standard curve, and calculating to obtain the content of clothianidin and thiamethoxam. The method disclosed by the invention has the characteristics of uniform sample pretreatment, simplicity, convenience and rapidness in detection, high sensitivity, capability of simultaneously providing quantitative results of two items and the like, and is suitable for on-site screening of large-batch samples.

This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a reagent card positioning device for a fluorescence immunoassay analyzer. It includes an instrument assembly, a reagent card assembly, and a positioning assembly. The instrument assembly includes an analyzer body, the reagent card assembly includes a reagent card body, and the positioning assembly includes a linkage slide plate and a handle. A groove is formed on the inner side of the front end of the analyzer body, and a positioning inner post is formed on the inner side of the analyzer body. A reagent hole is provided on the inner side of the upper part of the reagent card body, and a positioning inner hole is formed on the inner side of the front wall of the reagent card body. One end of the linkage slide plate is connected to a positioning insertion rod. The analyzer body is designed for fluorescence immunoassay analysis of samples within the reagent card body. The elastic buffer pad absorbs the impact force when the reagent card body is inserted, preventing sample splashing or displacement due to collision within the reagent hole.

The utility model discloses a reaction device for heavy metal fluorescence immunoassay and a heavy metal detection system, and relates to the technical field of heavy metal detection, and the reaction device comprises a shell, a sample loading port, a probe inlet, a cleaning port and a liquid outlet. An inner cavity of the shell is used for enriching a functional solid-phase carrier of heavy metal ions, and at least part of the wall surface of the shell is made of a transparent material to form an optical detection window; the sample loading port is formed in the shell and is used for introducing a liquid sample to be detected into the inner cavity; the probe inlet is formed in the shell and is used for introducing a fluorescent probe coupled with a heavy metal specific antibody into the inner cavity; the cleaning opening is formed in the shell and used for introducing cleaning liquid into the inner cavity; the liquid outlet is formed in the shell and is used for discharging reacted liquid. The scheme provided by the utility model is simple to operate and low in cost.

The application discloses a kind of simultaneously identifying wild type (W-MPT64) and 63bp deletion mutant (D-MPT64) Mycobacterium tuberculosis secreted protein 64 monoclonal antibody.The antibody heavy chain and light chain variable region amino acid sequence as shown in SEQ ID NO.1 and SEQ ID NO.5.The Mycobacterium tuberculosis MPT64 double antibody sandwich enzyme-linked immunosorbent assay and fluorescence immunoassay method established using the monoclonal antibody reach 1.5625 pg / mL, significantly higher than the minimum detection limit of prior art, can be used for qualitative and quantitative detection of Mycobacterium tuberculosis secreted protein 64.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a reaction cup transfer device which comprises a mounting frame, an incubation disc and a cleaning disc which are rotationally arranged on the mounting frame, and an incubation lapping seat and a cleaning lapping seat which are respectively arranged at the lower ends of the incubation disc and the cleaning disc in an annular array, the stirring finger mechanism is arranged on the mounting frame and is positioned below the incubation disc and the cleaning disc; the shifting finger mechanism comprises a fixing frame, an overturning motor arranged on the fixing frame, an overturning shaft rotationally arranged on the fixing frame, an overturning sleeve arranged on the overturning shaft in a sleeving mode, a finger plate fixed to the overturning sleeve, a translation motor arranged on one side of the fixing frame and a driven belt wheel arranged on the other side of the fixing frame. The translation belt is arranged at the driving end of the translation motor and on the driven belt wheel, and the translation seat is connected with the translation belt. According to the design, the reaction cup can be stably pushed and transferred, the structure is simple, cost is low, and operation and control are convenient.

The invention provides a time-resolved fluorescence immunochromatography kit for detecting PEDF and a preparation method of the time-resolved fluorescence immunochromatography kit, and belongs to the technical field of in-vitro detection. The invention provides the kit for detecting the content of the pigment epithelial-derived factor (PEDF) by using the time-resolved fluorescence immunochromatography for the first time. The kit comprises a detection card, freeze-dried microspheres and a detection buffer solution, the freeze-dried microspheres comprise a fluorescently-labeled PEDF antibody I and a fluorescently-labeled first antibody; the detection card comprises a bottom plate, a sample pad, an analysis film and a water absorption film are sequentially overlapped on the bottom plate, a quality control line C and a detection line T are marked on the analysis film, and the freeze-dried microspheres are arranged on the sample pad. The kit can be used for rapidly, efficiently, stably and accurately detecting PEDF, and the detection method is simple to operate, rapid, efficient, high in precision and accuracy, low in cost and wide in application prospect.

This invention relates to the field of reagent testing technology, and in particular to a reagent card pushing device and a fluorescence immunoassay analyzer. The reagent card pushing device comprises a frame and a pushing unit. The frame includes a support platform and at least two limiting frames, which are fixed at intervals along the length of the frame to the support platform. Adjacent limiting frames form a placement space extending along the width of the frame. The pushing unit comprises a drive module and a pushing module, with the pushing module having a pushing end. When pushing reagent cards, the cartridge box is placed with its opening facing downwards in the placement space. The reagent card at the bottom will be pushed out of the cartridge box from its opening. The pushing module can move along the width of the frame by driving the pushing end of the pushing module, so that the pushing end pushes the reagent card in the cartridge box out of the placement space from its card outlet, thus facilitating the removal of the reagent card from the cartridge box and providing convenience for fluorescence immunoassay analysis in the fluorescence immunoassay analyzer.

The utility model discloses a fluorescence immunochromatography quantitative detector, which relates to the technical field of fluorescence immunoassay analyzers, and particularly comprises a detector, and a bracket for placing a reagent strip is arranged in a detection cavity; a top cover is detachably mounted at the top of the detection cavity, a third electric actuator is mounted at the top in the top cover, and a fluorescence receiving part is arranged at the lower end of the third electric actuator; an adjusting motor is arranged on one side of the mounting frame body, a rotating shaft of the adjusting motor is connected with the mounting seat body, a second electric actuator is arranged in the mounting seat body, a light source is mounted at the bottom of the telescopic end of the second electric actuator, and light beams emitted by the light source face the reagent strip and are received by the fluorescence receiving part after being reflected by the reagent strip. During use, the distance between the light source and the reagent strip is conveniently adjusted, light rays are concentrated on the reagent strip to the greatest extent, the third electric actuator can be controlled to adjust the distance between the fluorescence receiving part and the reagent strip, and the fluorescence receiving part can receive light signals at the optimal distance.

A horizontal light path groove, a vertical light path groove, a light source generation device and a dichroic mirror are arranged in a light path box body, the vertical light path groove is provided with a first lens assembly and a second lens assembly at the two ends of the dichroic mirror respectively, the first lens assembly comprises a first plano-convex lens and a cylindrical mirror, and the second lens assembly comprises a second plano-convex lens and a second plano-convex lens. The cylindrical mirror is arranged on the side, away from the dichroic mirror, of the first plano-convex lens, the cylindrical mirror can focus light into linear light spots, the problem of mutual interference between adjacent lines of the multi-union card is solved, the linear light spots can excite a single line, the numerical value reading accuracy is improved, and the peak curve recognition rate is increased; the second lens assembly comprises a second plano-convex lens and a third plano-convex lens, the third plano-convex lens is arranged on the side, away from the dichroic mirror, of the second plano-convex lens, the third plano-convex lens is additionally arranged, emitted light spots are parallel light, the light intensity and the light ray are evenly distributed through the secondary convex lens, and the numerical value reading accuracy is improved.

This invention discloses D-glucuronic acid-modified Eu₂O₃ nanoparticles, their preparation method, and their application in cTnT detection. The experimental conditions were optimized to obtain the best D-glucuronic acid-modified Eu₂O₃ nanoparticles. This invention constructs a method for detecting cTnT using time-resolved fluorescence immunoassay and an immunochromatographic method for cTnT detection using DGA-Eu₂O₃ nanoparticles. The detection method of this invention exhibits high sensitivity, specificity, precision, accuracy, and recovery rate.

This invention relates to a droplet microfluidic-based PSA detection method, which solves the technical problems of high detection limit and low sensitivity in existing ELISA methods for detecting the prostate cancer biomarker PSA. The method first prepares an oil phase, then mixes an immune complex suspension and FDG substrate to prepare an aqueous phase, then generates and captures droplets in a flow-focusing microfluidic chip to form a monolayer droplet array, and finally incubates and performs fluorescence immunoassay to achieve quantitative detection of PSA.

This invention discloses an adaptive feature extraction method and system for quantitative fluorescence immunoassay, relating to the field of fluorescence immunoassay technology. The method includes: acquiring a sequence of fluorescence speckle images; calculating the image gradient difference between adjacent frames; constructing a three-dimensional interferometric perturbation tensor; and obtaining the principal perturbation mode map through sparse principal component analysis. Based on the principal perturbation mode map, a self-supervised triplet training sample is constructed, and the perturbation value is used as a pixel-level weighting factor to train an image patch encoder. The trained encoder is used to extract image patch features, which are then matched with a response feature dictionary to generate a response probability map. The perturbation spectral entropy of each time-series map is calculated, and its reciprocal is used for weighted fusion to generate a fused response probability map. The result is output through mask screening and response integral calculation, using a fitting method. This invention effectively enhances the local immune response recognition capability, improves the adaptability of feature extraction under perturbation, and enhances the stability of response fusion, achieving high-precision quantitative fluorescence immunoassay.

The utility model relates to the technical field of medical detection equipment, and discloses a reagent card magazine and a fluorescence immunoassay analyzer. The reagent card magazine comprises a magazine shell and a balancing weight. The magazine shell is provided with a containing cavity used for stacking and containing reagent cards, a card outlet is formed in the lower portion of the magazine shell, a driving groove is formed in the bottom of the shell, and the magazine shell is suitable for allowing an external card outlet driving piece to stretch into the containing cavity through the driving groove so as to drive the reagent cards located on the bottom layer to be discharged from the card outlet; the balancing weight is arranged in the magazine shell, the balancing weight is movably arranged in a containing cavity of the magazine shell, a non-return structure is arranged between the balancing weight and the magazine shell, the non-return structure comprises a limiting step arranged on the inner wall of the magazine shell and an elastic locking piece arranged on the balancing weight, and the elastic locking piece and the limiting step are locked in a one-way mode. Therefore, the balancing weight is suitable for doing one-way movement close to the driving groove. The reagent card magazine provided by the utility model is simple in structure and stable and convenient in card discharging.

The invention discloses a tetrahedral DNA-rare earth fluorescent nanoparticle composite probe and a preparation method and application thereof, and relates to the technical field of nanomaterials, the composite probe comprises rare earth fluorescent nanoparticles, the surface of which is fixed with an antibody and streptavidin; one vertex of the tetrahedral DNA framework is modified with biotin, and the tetrahedral DNA framework is modified on the surface of the rare earth fluorescent nanoparticle through specific binding between the biotin and the streptavidin. The composite probe can be used for preparing a detection reagent, detection test paper or a detection kit for time-resolved fluorescence immunoassay so as to improve the detection specificity and stability.

The invention provides an intelligent multi-channel fluorescence immunochromatography analyzer, which belongs to the technical field of optical immunochromatography analyzers and comprises a shell, a driving motor and a power transmission component, wherein the driving motor and the power transmission component are used for providing operation power for mechanical components in the shell; the optical module is used for emitting and receiving detection light; a base is arranged in the shell, a plurality of slots used for containing colloidal gold are fixedly mounted above the base, and the slots can be customized according to specific requirements of customers so as to be suitable for different numbers of multi-connected cards or single cards; the optical module comprises a vertical light path module, a transverse light path module and a mounting plate for supporting the vertical light path module and the transverse light path module; the chromatographic analyzer provided by the invention can solve the problems that equipment needs to be readjusted when fluorescence immunoassay detection and colloidal gold detection are carried out at the same time, and time and labor are wasted at present.