4 results about "Fluorescence immunoassay" patented technology

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

This invention discloses a fluorescence immunoassay analyzer, belonging to the technical field of fluorescence immunoassay devices, designed to solve the problem of continuous sample injection in existing devices. The fluorescence immunoassay analyzer disclosed in this invention includes: a housing; a rotating sample holder including a rotating stage with at least two sample slots for holding sample tubes, the sample tubes rotating synchronously with the rotating stage; a vortex oscillation mechanism for vortexing and oscillating the sample tubes; a testing mechanism; a transport mechanism for transporting the sample tubes between the rotating sample holder and the vortex oscillation mechanism; and a pipetting mechanism for adding or extracting liquid from the sample tubes. The fluorescence immunoassay analyzer disclosed in this invention includes a rotating stage, allowing the next batch or more batches of sample tubes to be placed on the rotating stage and injected with extraction liquid while the previous batch of sample tubes is vortexed on the vortex oscillation mechanism, achieving continuous sample injection and high detection efficiency.

This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a reagent card positioning device for a fluorescence immunoassay analyzer. It includes an instrument assembly, a reagent card assembly, and a positioning assembly. The instrument assembly includes an analyzer body, the reagent card assembly includes a reagent card body, and the positioning assembly includes a linkage slide plate and a handle. A groove is formed on the inner side of the front end of the analyzer body, and a positioning inner post is formed on the inner side of the analyzer body. A reagent hole is provided on the inner side of the upper part of the reagent card body, and a positioning inner hole is formed on the inner side of the front wall of the reagent card body. One end of the linkage slide plate is connected to a positioning insertion rod. The analyzer body is designed for fluorescence immunoassay analysis of samples within the reagent card body. The elastic buffer pad absorbs the impact force when the reagent card body is inserted, preventing sample splashing or displacement due to collision within the reagent hole.

This invention relates to a droplet microfluidic-based PSA detection method, which solves the technical problems of high detection limit and low sensitivity in existing ELISA methods for detecting the prostate cancer biomarker PSA. The method first prepares an oil phase, then mixes an immune complex suspension and FDG substrate to prepare an aqueous phase, then generates and captures droplets in a flow-focusing microfluidic chip to form a monolayer droplet array, and finally incubates and performs fluorescence immunoassay to achieve quantitative detection of PSA.