14 results about "Fluorescence immunoassay" patented technology

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

The utility model relates to the technical field of quantum dot fluorescence immunoassay analyzers, in particular to a detection platform for a quantum dot fluorescence immunoassay analyzer. The detection platform for the quantum dot fluorescence immunoassay analyzer comprises a detection table and an immunoassay analyzer body mounted on one side of the detection table, and the immunoassay analyzer body is provided with a detection window for placing a sample for detection; the control box is mounted on the other side of the detection table; the stand column is fixedly installed on the detection table and located on one side of the detection window, a sleeve is rotationally installed on the stand column, a hollow annular disc is fixedly installed at the top end of the sleeve, a plurality of through grooves are evenly formed in the annular disc, supporting shafts are rotationally installed in the through grooves through damping sleeve shafts, and bearing blocks are fixedly installed on the supporting shafts; the upper end and the lower end of the bearing block are each provided with a containing assembly used for containing a sample. The detection platform for the quantum dot fluorescence immunoassay analyzer provided by the utility model has the advantage of high efficiency and rapidness in detection.

The invention relates to the technical field of detection equipment, and discloses a biochemical blood coagulation and immunity integrated analyzer which comprises a shell, a biochemical blood coagulation detection module and an immunity detection module which are arranged in the shell, a biochemical blood coagulation detection port and an immunity detection port which are formed in the front surface of the shell, and a baffle plate arranged at an opening of the biochemical blood coagulation detection port, the heat dissipation channel is formed in the side face of the shell, and the biochemical blood coagulation detection module extends out of the immunodetection module. According to the biochemical blood coagulation and immunity integrated analyzer, the biochemical blood coagulation detection module and the immunity detection module are integrated in the shell, multiple devices do not need to detect for several times, integrated detection of biochemical, blood coagulation and immune markers is achieved, the detection efficiency is greatly improved, and the medical cost is reduced; based on the advantages of portability of microfluidics and high sensitivity of fluorescence immunoassay, POCT scene requirements are met, the sample dosage and detection time consumption are reduced, and support is provided for rapid clinical diagnosis.

The invention discloses a fluorescence immunoadsorption method for detecting an O-type foot-and-mouth disease virus VP1 protein antibody and application, and relates to the technical field of genetic engineering, and the method is characterized by comprising the following steps: S1, preparing O-type foot-and-mouth disease virus VP1 protein by using a prokaryotic expression system; s2, preparing an anti-O type foot-and-mouth disease VP1 protein monoclonal antibody capable of recognizing a VP1 protein epitope; s3, coupling quantum dots with the anti-O-type foot-and-mouth disease VP1 protein monoclonal antibody to obtain a novel fluorescent probe; and S4, quantitatively detecting the O-type foot-and-mouth disease virus antibody through a fluorescence immunoadsorption method. According to the method, quantum dots and an O-type foot-and-mouth disease VP1 protein resisting monoclonal antibody are coupled to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of the O-type foot-and-mouth disease virus antibody, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay, the method is more sensitive.

This invention discloses a fluorescence immunoassay analyzer, belonging to the technical field of fluorescence immunoassay devices, designed to solve the problem of continuous sample injection in existing devices. The fluorescence immunoassay analyzer disclosed in this invention includes: a housing; a rotating sample holder including a rotating stage with at least two sample slots for holding sample tubes, the sample tubes rotating synchronously with the rotating stage; a vortex oscillation mechanism for vortexing and oscillating the sample tubes; a testing mechanism; a transport mechanism for transporting the sample tubes between the rotating sample holder and the vortex oscillation mechanism; and a pipetting mechanism for adding or extracting liquid from the sample tubes. The fluorescence immunoassay analyzer disclosed in this invention includes a rotating stage, allowing the next batch or more batches of sample tubes to be placed on the rotating stage and injected with extraction liquid while the previous batch of sample tubes is vortexed on the vortex oscillation mechanism, achieving continuous sample injection and high detection efficiency.

The invention discloses a method for detecting clothianidin and thiamethoxam in ginger based on quantum dot microsphere fluorescence immunochromatography, and belongs to the technical field of compound detection. The method comprises the following steps: (a) extracting a ginger sample by adopting an acetonitrile solution, and diluting supernate by using a PBS (Phosphate Buffer Solution) to obtain a solution to be detected; (b) dropwise adding a solution to be detected onto the sample pad of the test strip, incubating, reading a fluorescence signal by using a quantum dot microsphere fluorescence immunoassay analyzer, respectively obtaining signal intensities and ratios of detection lines and quality control lines of clothianidin and thiamethoxam, correspondingly substituting the ratios into a clothianidin and thiamethoxam standard curve, and calculating to obtain the content of clothianidin and thiamethoxam. The method disclosed by the invention has the characteristics of uniform sample pretreatment, simplicity, convenience and rapidness in detection, high sensitivity, capability of simultaneously providing quantitative results of two items and the like, and is suitable for on-site screening of large-batch samples.

This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a reagent card positioning device for a fluorescence immunoassay analyzer. It includes an instrument assembly, a reagent card assembly, and a positioning assembly. The instrument assembly includes an analyzer body, the reagent card assembly includes a reagent card body, and the positioning assembly includes a linkage slide plate and a handle. A groove is formed on the inner side of the front end of the analyzer body, and a positioning inner post is formed on the inner side of the analyzer body. A reagent hole is provided on the inner side of the upper part of the reagent card body, and a positioning inner hole is formed on the inner side of the front wall of the reagent card body. One end of the linkage slide plate is connected to a positioning insertion rod. The analyzer body is designed for fluorescence immunoassay analysis of samples within the reagent card body. The elastic buffer pad absorbs the impact force when the reagent card body is inserted, preventing sample splashing or displacement due to collision within the reagent hole.

The utility model discloses a reaction device for heavy metal fluorescence immunoassay and a heavy metal detection system, and relates to the technical field of heavy metal detection, and the reaction device comprises a shell, a sample loading port, a probe inlet, a cleaning port and a liquid outlet. An inner cavity of the shell is used for enriching a functional solid-phase carrier of heavy metal ions, and at least part of the wall surface of the shell is made of a transparent material to form an optical detection window; the sample loading port is formed in the shell and is used for introducing a liquid sample to be detected into the inner cavity; the probe inlet is formed in the shell and is used for introducing a fluorescent probe coupled with a heavy metal specific antibody into the inner cavity; the cleaning opening is formed in the shell and used for introducing cleaning liquid into the inner cavity; the liquid outlet is formed in the shell and is used for discharging reacted liquid. The scheme provided by the utility model is simple to operate and low in cost.

The application discloses a kind of simultaneously identifying wild type (W-MPT64) and 63bp deletion mutant (D-MPT64) Mycobacterium tuberculosis secreted protein 64 monoclonal antibody.The antibody heavy chain and light chain variable region amino acid sequence as shown in SEQ ID NO.1 and SEQ ID NO.5.The Mycobacterium tuberculosis MPT64 double antibody sandwich enzyme-linked immunosorbent assay and fluorescence immunoassay method established using the monoclonal antibody reach 1.5625 pg / mL, significantly higher than the minimum detection limit of prior art, can be used for qualitative and quantitative detection of Mycobacterium tuberculosis secreted protein 64.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

This invention relates to a droplet microfluidic-based PSA detection method, which solves the technical problems of high detection limit and low sensitivity in existing ELISA methods for detecting the prostate cancer biomarker PSA. The method first prepares an oil phase, then mixes an immune complex suspension and FDG substrate to prepare an aqueous phase, then generates and captures droplets in a flow-focusing microfluidic chip to form a monolayer droplet array, and finally incubates and performs fluorescence immunoassay to achieve quantitative detection of PSA.