267 results about "Fluorescence immunoassay" patented technology

The invention discloses a class of fluorescent nanoparticles and a preparation method and an application thereof. The fluorescent nanoparticle comprises matrix material and fluorescent dye dispersed in the matrix material, wherein the matrix material is the compound composed of hydrophobic polymer main chain of which is hydrocarbon chain, organo-siloxane and organosilicon-polymer, and the fluorescent dye is rare earth complex with photoluminescence performance. The preparation method of the fluorescent nanoparticle comprises the following steps: dissolving rare earth complex, hydrophobic polymer and compound RSi (OR') 3 into organic solvent miscible with water; adding the mixture into aqueous solution containing surfactant; using precipitation and hydrolytic condensation reaction to form nanoparticles. The nanoparticle of the invention has favourable luminous performance and favourable stability, can have a silicon oxide shell and a surface functional group and can be used for bondingbiomolecules; biological probes based on the fluorescent nanoparticle have wide application prospects on the aspects of high-sensitivity fluorescence immunoassay, imaging and the like.

The invention relates to a novel, quick, high-sensitivity, qualitative and quantitative clinical test method which uses a chemically modified functional polymorphic substrate as media and combines inorganic fluorescent semiconductor nano materials (quantum dots) and immunology means. The multi-functional quick fluorescence immunoassay test method using a functional substrate as media and using single color and multi-color quantum dots as a mark is suitable for a single or compound qualitative and quantitative test of various hormone, protein, nucleic acid and pathogens. Compared with traditional enzyme-linked immunoassay and colloidal gold test paper, the multi-functional quick fluorescence immunoassay test method has the advantages of being simple, quick, high in sensitivity, long in storage time and the like. The multi-functional quick fluorescence immunoassay test method can meet requirements of different testing conditions and samples, can conduct the single or compound qualitative and quantitative test on different antigens on the same substrate media at the same time, and has great potential on aspects of clinical applications.

The invention provides a kit suitable for rapidly detecting AMH and INHB by using a double-tagging time resolution fluorescence immunoassay method. The kit comprises (1) a solid phase carrier coated by an AMH coating antibody and an INHB coating antibody; (2) a mixed calibration product of AMH and INHB; (3) an AMH detection antibody marked by using a lanthanide element 1; (4) an INHB detection antibody marked by a lanthanide element 2; (5) an immunoreaction accelerant liquid; (6) a concentrated washing liquid; and (7) a reinforcing liquid. The invention further provides a use method of the kit for rapidly detecting AMH and INHB by using the double-tagging time resolution fluorescence immunoassay method. By adopting a double-tagging time resolution fluorescence immunoassay technique, the defect that conventional AMH and INHB respectively need a single kit, that is, one kit can be only used for detecting AMH or INHB can be overcome, the detection steps can be reduced, the sampling time can be shortened, and the use amount of a blood sample can be reduced.

The invention discloses a piece of fluorescence immunoassay chromatography test paper which is rapid, sensitive, simple and convenient to operate to test the residual quantity of cefalexin, and preparation of the test paper. The test paper comprises a sample pad, a binding pad, a nitrocellulose membrane and a water absorbing pad, which are adhered to a substrate in a mutual lap joint manner in sequence, wherein the nitrocellulose membrane is coated with a detection line and a quality control line; the binding pad is coated with a cefalexin monoclonal antibody with a fluorescent mark. The preparation method of the fluorescence immunoassay chromatography test paper for cefalexin residue comprises the following steps: synthesizing cefalexin-ovalbumin coating antigen, preparing a goat anti-mouse immune globulin antibody, coating the cefalexin-ovalbumin coating antigen and the goat anti-mouse immune globulin antibody onthe nitrocellulose membrane to serve as the detection line and the quality control line, preparing a fluorescence nano-particle mark cefalexin monoclonal antibody, then coating glass fiber to serve as the binding pad, sequentially adhering the sample pad, the binding pad, the nitrocellulose membrane and the water absorbing pad to a back plate in the lap joint manner in sequence, cutting the obtained test paper into the width of 4mm, and preserving at normal temperature.

The invention belongs to technical field of immunoassay, and particularly relates to a preparation method of a signal amplifying type quantum dot immune fluorescent probe and an application of the signal amplifying type quantum dot immune fluorescent probe. At first, According to the method, a microemulsion solvent evaporation method is adopted for preparing a quantum dot-polymer composite nanosphere with controllable grain size and good dispersibility by utilizing quantum dot and polymer solution; and an antibody is covalently coupled on the surface of the composite nanosphere to obtain the signal amplifying type quantum dot immune fluorescent probe. The invention provides an application of the quantum dot immune fluorescent probe in a fluorescence immunoassay method or a fluorescence immunoassay detection kit. Compared with traditional quantum dot immune fluorescent probe, the quantum dot immune fluorescent probe prepared by the method provided by the invention has stronger signals and good detection sensitivity.

Disclosed is a method of quantum dot-labeled indirect competitive fluorescence immunoassay of betamethasone, which belongs to the immunoassay method technique field. Quantum dots for labeling antibodies of the invention have the emission spectra of QD590, and the method comprises: directly covering coating antigens in micro-holes of an enzyme label plate, adding betamethasone standard solution or a sample under test to form an antigen-antibody fluorescence immunity compound body, stimulating and detecting the fluorescence intensity of the formed antigen-antibody fluorescence immunity compound body with a fluorescence enzyme-labeling instrument, and obtaining the concentration of betamethasone in the sample under test through comparing with the standard solution. The invention can detect the content of betamethasone in the sample under test without adding chromogenic substance, namely, the concentration of betamethasone in the sample under test can be detected indirectly through the fluorescence intensity of the antigen-antibody immunity compound body, and both the operation and reaction need only one step; and the quantum dots for labeling antibodies of the invention have advantages of stronger emitted fluorescence intensity and long stabilization time of fluorescence compared with the traditional fluorescence.

The invention relates to fluorescence immunoassay detection method and kit for HER-2. The method comprises the following steps: preparation of a probe fixing pad; preparation of an immunoassay test strip; preparation of sample diluent; and inspection of the sample. HER-2 monoclonal antibody fluorescence latex particles and goat anti-rabbit IgG fluorescence latex particles are mixed and then diluted by using gold diluent, and the diluted mixture is sprayed on glass fiber so as to prepare the probe fixing pad; and a detection line is coated by an HER-2 monoclonal antibody, and a control line is coated by goat anti-rabbit IgG so as to prepare the fluorescence immunoassay test strip. The detection kit provided by the invention comprises a PVC liner plate, a sample pad, the probe fixing pad, a cellulose nitrate membrane and absorbent paper. The probe fixing pad is prepared by mixing and drying the HER-2 monoclonal antibody fluorescence latex particles and the goat anti-rabbit IgG fluorescence latex particles. Thus, the fluorescence immunoassay detection method and kit have the advantages of high sensitivity, high accuracy, simple operation and low cost.

The invention relates to fluorescent nanoparticles Ru(bpy)3/SiO2, a preparation method and application thereof. The fluorescent nanoparticles have nuclear shell structures; the nuclear shell structure is formed by taking tris(2,2'-bipyridyl)ruthenium as a core, covering silicon dioxide with netlike structure on the surface of the tris(2,2'-bipyridyl)ruthenium and carrying active amino groups on the surface of the silicon dioxide, wherein the mass ratio of the tris(2,2'-bipyridyl)ruthenium to the silicon dioxide is 1:5 to 1:8; and every milligram of nanoparticles comprises 385nmol of amino group. The silicon fluorescent nanoparticles Ru(bpy)3/SiO2 have the advantages of uniform size, high monodispersity, mean diameter of 70+/-6nm, high light stability and difficult dye leakage in aqueous solution. The fluorescent probe is applied to a protein microarray chip to detect HIV p24 antigen after marking streptavidin; the analysis method is a sandwich fluorescence immunoassay method; and the result shows that the fluorescence intensity is in good positive relationship with p24 concentration and the analytical sensitivity is 3.1ng/mL. The result shows that the nanoparticles, serving as a novel fluorescent probe, can be applied to the systems of the protein microarray chip and fluorescence immunoassay and the like for high flexibility detection.

The invention belongs to the field of immuno-chromatographic detection, and in particular relates to a quantitative detection and computation method based on the fluorescence immuno-chromatographic technology, comprising the following steps: inserting a fluorescence immunostrip dripped with a sample solution to be detected into a fluorescence immunoassay instrument, and sequentially performing irradiation with an LED light source, optical filtering processing with an optical filter, detection with a photoelectric detector, electrical signal processing, AD conversion processing, baseline correction, L-M algorithm processing, area computation and concentration computation. The quantitative detection and computation method stresses on signal reconstruction by using an algorithm, eliminates influence of noise and various interferences, ensures the signal processing course undistorted, and can obtain high-precision and low-error results, thereby quickly, conveniently and accurately determining the concentration result.

The invention discloses a fully-automatic fluorescence immunoassay system comprising a suction head placing frame, a sample uniformly-shaking unit, a buffer solution feeding unit, a test paper sheet conveying unit, a three-dimensional sample adding unit, a detection unit and a signal processing control unit, wherein a suction head is placed on the suction head placing frame; the sample uniformly-shaking unit is used for uniformly shaking a to-be-detected sample; the buffer solution feeding unit is used for conveying a buffer solution plate to a working position, and the buffer solution plate is abandoned after running out; the test paper sheet conveying unit is used for pushing out test paper sheets from a test paper box and respectively conveying the test paper sheets to a sample adding position and a detection position; the three-dimensional sample adding unit is used for loading the suction head; and the detection unit is used for detecting the test paper sheets which are subjected to sample adding, and the test paper sheets are abandoned after detection ends. According to the fully-automatic fluorescence immunoassay system disclosed by the invention, the strength and error rate of detection operations can be significantly reduced by virtue of fully-automatic operations, the detection throughput can reach 60 tests per hour, and thus the requirements of large batch detection can be met; and according to the fully-automatic fluorescence immunoassay system, the suction and mixing of the samples and the sample adding of the test paper sheets are performed by using the disposable suction heads, so that the cross contamination among the samples can be avoided, and the accuracy of results can be improved.

The invention discloses a method for detecting imidacloprid by quantum-dot-marked sandwich fluorescence immunoassay. According to the technical scheme, the method comprises the following steps of: 1, preparing a nuclear shell quantum dot and modifying the water solubility of a fat-soluble quantum dot by modification of different macromolecules; 2, preparing and passivating a quantum probe; and 3, constructing an imidacloprid antibody quantum dot immunoassay method. The method is applied to preparation, passivation and performance representation of the quantum dot and detection of precipitate imidacloprid residues and is a fluorescence immunoassay method for sensitively and quickly detecting the imidacloprid in vegetables based on the quantum dot and conjugates of IgG-HRP.

The invention relates to the field of medical detection and relates to a biochemical and immunological analysis meter and a biochemical and immunological detecting method. The biochemical and immunological analysis meter comprises a test board, a supporting base, a fluorescent scanning light path component and a display component; a reagent card which is fixedly inserted into a supporting part is subjected to dry biochemical analysis, and the fluorescent scanning light path component can be driven by a driving mechanism to drive a moving component to slide along the length direction of a groove, thereby carrying out fluorescent scanning analysis on the fixed reagent card and outputting a detection result by the display component. The biochemical and immunological analysis meter has the functions of fluorescence immunoassay quantitation and dry biochemical analysis, the range of detection items is widened, and detection time is saved. According to the method, the biochemical and immunological analysis meter is adopted for detecting, and further simultaneous measurement of fluorescence immunoassay quantitative analysis and dry biochemical quantitative analysis is realized; in addition, two measurement methods are in mutually non-intervention and are separated from each other.

The invention relates to the technical field of fluorescence immunoassay in medical immunology, and in particular relates to a serumamyloid A (SAA) testing kit (fluorescence immunochromatographic method) and a manufacturing method. The kit is provided with a test paper card. The kit is characterized in that a PVC (Polyvinyl Chloride) plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorbing pad are sequentially arranged on the test paper card from bottom to top; a rare-earth Eu<3+> fluorescent microsphere marked serum amyloid A monoclonal antibody is adsorbed on the conjugate pad; the diameter of a rare-earth fluorescent microsphere is 200nm; the rare-earth fluorescent microsphere contains a rare-earth lanthanide element Eu<3+>; the rare-earth fluorescent microsphere is stable in a ground state and emits fluorescence of which the wavelength is 615nm under the stimulation action of laser of 337nm; and the monoclonal antibody is a monoclonal antibody which is purified and mixed, and comes from a monoclonal antibody cell strain for 2-6 different serum amyloid A epitopes. The kit has the advantages of being simple and convenient to operate, rapid in reaction, high in sensitivity, good in specificity and the like.

The invention provides a fluorescence immunoassay test strip used for detecting a tumor marker CA72-4 and a preparation method of the fluorescence immunoassay test strip. The test strip comprises a sample cushion, a quantum dot marker combination pad, a nitrocellulose membrane and a piece of water absorption paper which are sequentially laid on a bottom plate in an overlapped mode. The double-antibody sandwich immunochromatographic method is adopted. A CA72-4 resistant monoclonal antibody CC49 coating provided with a quantum dot marker is arranged on the quantum dot marker combination pad. A detection line and a quality control line are arranged on the nitrocellulose membrane. A CA72-4 monoclonal antibody B72.3 is arranged on the detection line. A goat-anti-mouse IgG is arranged on the quality control line. The preparation method mainly relates to preparation of the quantum dot marker antibody. After immunochromatography is conducted through the test strip, the quantitative result of CA72-4 is obtained by conducting analysis through a fluorescence immunochromatographic chip detection instrument, operation is easy and convenient, bedside timely and rapid detection can be conducted, and stability and flexibility are high.

A pentamethine cyanine fluorescent dye with N-substituting at β-position of conjugated chain is disclosed. The dye reacts with biosubstrate including protein, liposome and DNA, and shows excellent selectivity. It has good living cell membrane permeability and shows good selectivity in living cell staining In addition, the dye has good stability and low background fluorescence, and can be used together with other fluorescent dyes having consistent wavelengths for single-channel excitation and multi-channel detection. The dye can be used in the fields of protein labeling and detecting, fluorescence immunoassay and living cell selective imaging and the like.

The present invention relates to a porcine trichinosis antibody test strip, a preparation method and application thereof, and belongs to the technical field of fluorescence immunoassay. In order to detect trichinella infection more quickly, stably and accurately, the present invention provides a porcine trichinosis antibody test strip. The porcine trichinosis antibody test strip comprises a samplepad, a binding pad, a chromatographic membrane, an absorbent pad and a bottom plate; the binding pad is marked with time-resolved fluorescent microspheres coupled with the goat anti-porcine IgG; andthe chromatographic membrane is provided with a detection line and a quality control line, wherein the detection line is sprayed with the trichinella cocktail antigen composed of a recombinant musclelarval stage Ts-WM5 antigen expressed by prokaryotic cells, an intestinal infectious larval stage Ts-WN10 antigen, an adult stage Ts-ZH68 antigen and a newborn larval stage Ts-T668-C antigen, and thequality control line is sprayed with the rabbit anti-goat IgG. The test strip is prepared after combination of the components. The technical scheme of the present invention can be used for early rapiddetection of porcine trichinella infection.

The invention discloses a kit and detection method for mycoplasma pneumoniae IgM on the basis of a fluorescence immunoassay chromatographic technique.The method comprises the steps that fluorescein serves as a marking material, and a mouse antihuman IgM mu chain monoclonal antibody is marked.A mycoplasma pneumoniae specific IgM antibody in serum is combined with a fluorescein marked antibody to form a reaction compound which is caught by a mycoplasma pneumoniae antigen, the mycoplasma pneumoniae IgM catch yield is in positive correlation with signal strength of the fluorescent antibody, and the mycoplasma pneumoniae IgM in the serum can be detected out by means of an immunofluorescence detector reading.The method has the advantages of being short in time, good in specificity, good in accuracy and stability and the like, and the coincidence rate of the detection result with that of a European Union mycoplasma pneumoniae IgM detection kit is high.A new method for rapidly and accurately detecting the mycoplasma pneumoniae IgM in clinic is provided, and a good market prospect is achieved.

The invention provides a fluorescence immunoassay kit used for detecting porcine circovirus type 2 (PCV2), which comprises a single factor serum antibody used for detecting PCV2, an FITC (fluorescein isothiocyanate) labeled goat anti-mouse IgG (immunoglobulin G), a positive control sample, stationary liquid and PBS (phosphate buffer) washing liquor, wherein the single factor serum antibody used for detecting PCV2 is prepared from a PCV2 Cap protein antigen immunized mouse with an amino acid sequence of SEQ ID NO: 1. The main ingredient in the kit used for detecting PCV2 prepared in the invention is a single factor serum antibody of mouse anti-porcine circovirus type 2 and is prepared from the antigen immunized mouse with the amino acid sequence of SEQ ID NO: 1, and the prepared antibody can generate a specific reaction with PCV2. The fluorescence immunoassay kit of porcine circovirus type 2 created on the basis of the single factor serum can be used for detecting antigen in the diseased tissues of an PCV2 infected pig on clinic and judging whether the pig is infected with PCV2, and the fluorescence immunoassay kit is strong in specificity, high in sensitivity, good in repeatability and high in detection speed.

The invention discloses a fluorescence immunoassay chromatography test strip for detecting vomitoxin. The fluorescence immunoassay chromatography test strip comprises a supporting body and an adsorption layer fixed to the supporting body. The adsorption layer sequentially comprises an adsorption fiber layer, a fluorescent antibody fiber layer, a cellulose membrane layer and a water absorption material layer starting from the test end. Invisible detection prints printed by a coupling DON carrier protein solution and invisible contrast prints printed by a goat anti-mouse IgG or rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solution are arranged on the cellulose membrane layer. The fluorescent antibody fiber layer is made from glass fiber cotton for adsorbing fluorescent antibodies. The fluorescent antibodies are graphene oxide fluorescent nanometer materials or NaYF4:Yb or Tm nano-particles or DON monoclonal antibodies or polyclonal antibodies marked by NaGd(WO4)2:Eu3+ nano-particles. The test strip has the advantages of being high in specificity, sensitivity and stability, good in safety and easy, convenient and rapid to use, can achieve on-site quantitative detection under a portable fluorescent reading device and can meet the requirements of people of different levels.

The invention discloses a monochromatic fluorescence detection device. The monochromatic fluorescence detection device comprises a photoelectric detector, a micropore diaphragm, a focusing lens, a first optical filter, a dichroic mirror, a parabolic reflector, a second optical filter, a collimating lens and an LED light source. The photoelectric detector, the micropore diaphragm, the focusing lensand the first optical filter are sequentially arrayed in an optical axis coincided manner to form a receiving light path. Optical axes of the dichroic mirror and the receiving light path form a 45-degree angle horizontally. The second optical filter, the collimating lens and the LED light source behind the dichronic mirror are sequentially arrayed in an optical axis coincided manner to form an excitation light path. The detection device for temporal separation of C line and T line optical signals on the basis of an optical path conversion structure of a parabolic reflector micromotion mechanism is small in size, high in measurement precision, portable and convenient for onsite detection and can be applied to researching of fluorescence immunoassay quantitative detection instruments.

The invention discloses a method for quantitatively detecting PSI-OAm-NAPI amphiphilic polymer nano-drug carriers based on trace fluorescence immunoassay. The method comprises the following steps: hydrolyzing PSI-OAm-NAPI to prepare and obtain immune antigens and coating antigens of MFAP, diluting the MFAP coating antigens through a coating solution and then coating the MFAP coating antigens in an elisa plate, sealing, adding MFAP standard products with different concentrations, taking FITC-labeled MFAP antibodies as primary antibodies, and building a direct competition fluorescence immunoassay quantitative detection MFAP. The method is capable of making full use of high specificity of the antigens and antibodies in the immunologic reaction and combining the high specificity of the antigens and the antibodies with sensibility of fluorescence, so that signals of the immunoreactions are further improved; the fluorescence intensity is used for carrying out quantitative analysis; the method is convenient and rapid, is low in detection cost, high in specificity and high in sensitivity, and is capable of carrying out high-flux measurement.