48 results about "Fluorescence immunoassay" patented technology

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The present invention discloses a preparation method and application of a composite probe for gold nanoclusters emitting enhanced fluorescence induced by the spatial confinement effect of ZIF-8 surface. HAuCl4 and glutathione are dissolved in water, the two solutions are mixed and reacted, and purified to obtain an AuNCs solution; Zn(NO3)2·6H2O and 2-methylimidazole are dissolved in methanol, the two solutions are mixed and reacted, and the generated white precipitate is treated to obtain a zeolite imidazole framework-8 solution; the obtained AuNCs solution and the zeolite imidazole framework-8 solution are mixed, stirred, self-assembled, and treated to obtain ZIF-8@(Au) nanoparticles; zinc nitrate and dimethylimidazole are added to the nanoparticle solution for reaction, and after treatment, a sandwich ZIF-8@Au@ZIF-8 nanomaterial is obtained; the nanomaterial is mixed and treated with the AuNCs solution to obtain a ZIF-8@Au loaded with two layers of AuNCs; the operation is repeated to obtain a ZIF-8@(Au@ZIF-8) loaded with n layers of AuNCs. n The present invention significantly improves the effective utilization rate of assembly space and fluorescence performance, and realizes ultra-sensitive detection of fluorescence immunoassay.

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

The utility model discloses a reagent box for fluorescence immunoassay, which belongs to the technical field of biochemical detection.The reagent box for fluorescence immunoassay comprises a box body and a box door, and the box door is hinged to the box body; a plurality of ice cavities are formed in the pulling plate, and the ice cavities are filled with ice bags; wherein a gap is formed between every two ice cavities, the gaps are used for containing reagents, the reagents are attached to the outer side walls of the ice cavities, and the pulling plate freely slides in the box body and can stretch out of the box body; wherein the ice cavity is higher than the reagent; wherein the drawing plate is a structural member made of a low heat conduction coefficient, and the ice cavity is a structural member made of a high heat conduction coefficient material. According to the utility model, the technical problem that the transportation cost of the fluorescence immunoassay reagent is too high in the short-distance transportation process can be solved.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The present invention provides a temperature-corrected analyte detection method, and relates to the following steps: after a series of analyte calibrators with different concentrations are added into an immunoassay test strip, using an optical analyzer to measure, at each segmented temperature point, a signal value generated after the analyte calibrator of each concentration is captured on a detection pad of the immunoassay test strip; drawing a corresponding equation by taking a T / C value at each segmented temperature point as an X axis and the concentration of the analyte calibrator as a Y axis; and then, performing temperature segmentation according to n selected segmented temperature points, and in different temperature segments, using different formulas to perform calculation. The temperature-corrected analyte detection method can be used for more accurately detecting myocardial markers, upper respiratory tract viral infections, metabolic hormones, gastrointestinal panel markers, allergens, and the like on the basis of time-resolved fluorescence immunoassay.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a magnetic bead bearing plate sealing imaging mechanism which comprises a mounting bottom plate, a bearing mechanism and an imaging mechanism, the bearing mechanism comprises a bearing disc arranged on the mounting bottom plate, a bearing groove formed in the bearing disc, a shaft hole formed in the center of the bearing groove, a rotating shaft arranged in the shaft hole and a rotating motor arranged at the lower end of the bearing disc, the upper end of the rotating shaft penetrates through the shaft hole and extends upwards, and the lower end of the rotating shaft is in transmission connection with the rotating motor; the imaging mechanism comprises a supporting frame arranged at the lower end of the mounting bottom plate and a fluorescence microscope arranged on the supporting frame, an observation hole is formed in the bearing groove, and the lens end of the fluorescence microscope corresponds to the observation hole. The design is simple in structure, low in cost and stable in operation.

The utility model relates to the technical field of quantum dot fluorescence immunoassay analyzers, in particular to a detection platform for a quantum dot fluorescence immunoassay analyzer. The detection platform for the quantum dot fluorescence immunoassay analyzer comprises a detection table and an immunoassay analyzer body mounted on one side of the detection table, and the immunoassay analyzer body is provided with a detection window for placing a sample for detection; the control box is mounted on the other side of the detection table; the stand column is fixedly installed on the detection table and located on one side of the detection window, a sleeve is rotationally installed on the stand column, a hollow annular disc is fixedly installed at the top end of the sleeve, a plurality of through grooves are evenly formed in the annular disc, supporting shafts are rotationally installed in the through grooves through damping sleeve shafts, and bearing blocks are fixedly installed on the supporting shafts; the upper end and the lower end of the bearing block are each provided with a containing assembly used for containing a sample. The detection platform for the quantum dot fluorescence immunoassay analyzer provided by the utility model has the advantage of high efficiency and rapidness in detection.

The invention relates to the technical field of detection equipment, and discloses a biochemical blood coagulation and immunity integrated analyzer which comprises a shell, a biochemical blood coagulation detection module and an immunity detection module which are arranged in the shell, a biochemical blood coagulation detection port and an immunity detection port which are formed in the front surface of the shell, and a baffle plate arranged at an opening of the biochemical blood coagulation detection port, the heat dissipation channel is formed in the side face of the shell, and the biochemical blood coagulation detection module extends out of the immunodetection module. According to the biochemical blood coagulation and immunity integrated analyzer, the biochemical blood coagulation detection module and the immunity detection module are integrated in the shell, multiple devices do not need to detect for several times, integrated detection of biochemical, blood coagulation and immune markers is achieved, the detection efficiency is greatly improved, and the medical cost is reduced; based on the advantages of portability of microfluidics and high sensitivity of fluorescence immunoassay, POCT scene requirements are met, the sample dosage and detection time consumption are reduced, and support is provided for rapid clinical diagnosis.

The utility model provides a fluorescence immunoassay analyzer with a suction head tray positioning structure, and relates to the technical field of fluorescence immunoassay analyzers, the fluorescence immunoassay analyzer comprises a base assembly and a positioning detection mechanism, a clamping transfer part assembled by a bolt is arranged above one side of the base assembly, and the positioning detection mechanism is arranged above the other side of the base assembly. A positioning detection mechanism for bolt assembly is arranged on the top side of the base assembly, and the positioning detection mechanism comprises a sleeving base, a side shell, a spring rod set, a clamping piece, a limiting block, a lower sleeving rod, an inserting plate, a magnetic suction cup, a connecting pipe, an air pump and a discharging opening; the magnetic suction cup is arranged below the lower sleeve rod, the lower sleeve rod is connected to the side shell in an inserted mode, the inserting plate is fixed through cooperation of the spring rod set, the clamping piece and the limiting block, and the magnetic suction cup and the lower sleeve rod are effectively connected after a product is fixed. And finally, through output power of an air pump, a magnetic suction cup can effectively suck the lower sleeve rod tightly, so that the suction tightness degree of the position can be improved, and the position accuracy is guaranteed.

The present invention provides a temperature-corrected analyte detection method, which involves adding a series of analyte calibrators with different concentrations to an immunoassay strip, and then using an optical analyzer to measure the signal values generated after each concentration of analyte calibrator is captured on the detection pad of the immunoassay strip at each segmented temperature point. Then, with the T / C value at each segmented temperature point as the X-axis and the concentration of the analyte calibrator as the Y-axis, a corresponding equation is plotted; then, according to the selected n segmented temperature points, temperature segmentation is performed, and different formulas are used for calculation in different temperature segments. This temperature-corrected analyte detection method can be based on time-resolved fluorescence immunoassay and is used to more accurately detect myocardial markers, upper respiratory virus infections, metabolic hormones, digestive tract series markers, allergens, etc.

The invention belongs to the technical field of analysis and detection, and particularly discloses a capillary electrophoresis fluorescence immunoassay method for detecting abrus precatorius toxin. A fluorescein isothiocyanate (FITC) labeled abrus precatorius toxin antibody probe is prepared, a non-competitive mode is adopted, a sample solution and an FITC labeled antibody are subjected to an incubation reaction, and the FITC labeled antibody is relatively excessive. The FITC labeled antibody-antigen compound and the excessive FITC labeled antibody in the mixed solution are divided into different zones in the separation capillary tube according to different migration rates, and fluorescence signal detection is performed through a window of a laser-induced fluorescence detector in sequence. Qualitative and quantitative analysis is carried out on the abrus precatorius toxin through the fluorescence signal peak and the intensity of the compound. According to the present invention, the detection method of the abrus precatorius toxin fluorescence immunocapillary electrophoresis is clarified, the detection automation is improved while the detection time is reduced compared with the traditional immunization method, the linear range of the abrus precatorius toxin standard solution detection is 75.8-606.4 nM, and the detection limit is 75.8 nM.

The invention provides a preparation process of a Hep-2 cell extract suitable for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies and a preparation method of the test strip. The method comprises the following steps: transferring cultured Hep-2 cells into a PBS (Phosphate Buffer Solution), carrying out centrifugal separation on the obtained Hep-2 cell suspension to obtain Hep-2 cell agglomerates, carrying out ultrasonic treatment on a mixture of the Hep-2 cell agglomerates and the extraction buffer solution in an ice bath, centrifuging the mixture subjected to ultrasonic treatment, collecting supernatant, and passing the supernatant through a micro-filtration membrane to obtain filtrate, namely the Hep-2 cell extract. The extraction buffer solution is an HEPES buffer solution containing isopropanol, trehalose, bovine serum albumin and phenylmethylsulfonyl fluoride. The prepared HEP-2 cell extract is used for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies, and the detection sensitivity, the specificity and the reliability of a detection result can be considered at the same time.

The invention discloses a fluorescence immunoadsorption method for detecting an O-type foot-and-mouth disease virus VP1 protein antibody and application, and relates to the technical field of genetic engineering, and the method is characterized by comprising the following steps: S1, preparing O-type foot-and-mouth disease virus VP1 protein by using a prokaryotic expression system; s2, preparing an anti-O type foot-and-mouth disease VP1 protein monoclonal antibody capable of recognizing a VP1 protein epitope; s3, coupling quantum dots with the anti-O-type foot-and-mouth disease VP1 protein monoclonal antibody to obtain a novel fluorescent probe; and S4, quantitatively detecting the O-type foot-and-mouth disease virus antibody through a fluorescence immunoadsorption method. According to the method, quantum dots and an O-type foot-and-mouth disease VP1 protein resisting monoclonal antibody are coupled to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of the O-type foot-and-mouth disease virus antibody, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay, the method is more sensitive.

This invention discloses a fluorescence immunoassay analyzer, belonging to the technical field of fluorescence immunoassay devices, designed to solve the problem of continuous sample injection in existing devices. The fluorescence immunoassay analyzer disclosed in this invention includes: a housing; a rotating sample holder including a rotating stage with at least two sample slots for holding sample tubes, the sample tubes rotating synchronously with the rotating stage; a vortex oscillation mechanism for vortexing and oscillating the sample tubes; a testing mechanism; a transport mechanism for transporting the sample tubes between the rotating sample holder and the vortex oscillation mechanism; and a pipetting mechanism for adding or extracting liquid from the sample tubes. The fluorescence immunoassay analyzer disclosed in this invention includes a rotating stage, allowing the next batch or more batches of sample tubes to be placed on the rotating stage and injected with extraction liquid while the previous batch of sample tubes is vortexed on the vortex oscillation mechanism, achieving continuous sample injection and high detection efficiency.

The invention discloses a method for detecting clothianidin and thiamethoxam in ginger based on quantum dot microsphere fluorescence immunochromatography, and belongs to the technical field of compound detection. The method comprises the following steps: (a) extracting a ginger sample by adopting an acetonitrile solution, and diluting supernate by using a PBS (Phosphate Buffer Solution) to obtain a solution to be detected; (b) dropwise adding a solution to be detected onto the sample pad of the test strip, incubating, reading a fluorescence signal by using a quantum dot microsphere fluorescence immunoassay analyzer, respectively obtaining signal intensities and ratios of detection lines and quality control lines of clothianidin and thiamethoxam, correspondingly substituting the ratios into a clothianidin and thiamethoxam standard curve, and calculating to obtain the content of clothianidin and thiamethoxam. The method disclosed by the invention has the characteristics of uniform sample pretreatment, simplicity, convenience and rapidness in detection, high sensitivity, capability of simultaneously providing quantitative results of two items and the like, and is suitable for on-site screening of large-batch samples.

The invention relates to the technical field of nano biosensing, in particular to a mycoplasma bovis rapid detection method based on a nano antibody, which comprises the following steps: expressing a mycoplasma bovis specific nano antibody by gene-edited escherichia coli, and culturing in a culture medium containing a novel protein expression accelerant and a self-assembly inducer to obtain the mycoplasma bovis rapid detection method based on the nano antibody. Chemical marking is carried out through clicking by a special fluorescent marker; treating a sample with a surfactant and a nuclease inhibitor, centrifuging, taking supernatant, and reacting with the labeled nano antibody and the signal amplifying agent in the micro-fluidic chip; magnetic separation is combined with time-resolved fluorescence immunoassay, and a fluorescence signal ratio is detected through dual-wavelength excitation, so that whether the mycoplasma bovis exists in a sample is judged. The detection method is rapid in detection and can be completed within one hour; the accuracy is high, and misjudgment is avoided; the operation is simple and convenient; cost is low and popularization is easy; and high throughput and universality are achieved.

This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a reagent card positioning device for a fluorescence immunoassay analyzer. It includes an instrument assembly, a reagent card assembly, and a positioning assembly. The instrument assembly includes an analyzer body, the reagent card assembly includes a reagent card body, and the positioning assembly includes a linkage slide plate and a handle. A groove is formed on the inner side of the front end of the analyzer body, and a positioning inner post is formed on the inner side of the analyzer body. A reagent hole is provided on the inner side of the upper part of the reagent card body, and a positioning inner hole is formed on the inner side of the front wall of the reagent card body. One end of the linkage slide plate is connected to a positioning insertion rod. The analyzer body is designed for fluorescence immunoassay analysis of samples within the reagent card body. The elastic buffer pad absorbs the impact force when the reagent card body is inserted, preventing sample splashing or displacement due to collision within the reagent hole.

This application relates to the field of fluorescence immunoassay equipment, and provides a fully automatic fluorescence immunoassay device, which includes a cartridge base, a reagent cartridge, and a card pushing assembly; the cartridge base is provided with a card slot, and a first through groove is provided at the bottom of the card slot; the reagent cartridge is placed in the card slot, and the reagent cartridge includes a box body and a reagent card, the reagent card is placed in the box body, and the box body is provided with a material pushing notch and a discharge port; the card pushing assembly includes a card pushing unit, an X-direction driving unit, and a Z-direction driving unit, both the X-direction driving unit and the Z-direction driving unit are connected to the card pushing unit, the Z-direction driving unit is used to drive the card pushing unit to lift, and the X-direction driving unit is used to drive the card pushing assembly to push out the first reagent card; the card pushing unit includes a mounting block, a card pushing member, and a partition plate; the card pushing member is used to push the reagent card to move; the partition plate is located above the card pushing member and is used to be inserted under the second reagent card. This application solves the problems of low reagent card supply efficiency and easy jamming phenomenon.

The present invention constructs a method for a three-enzyme cascade-triggered fluorescence immunosensing platform by sequentially integrating ALP, TYR, and HRP. This platform relies on the fact that HRP and H2O2 can promote the rapid in-situ fluorescence reaction of dopamine and 1,5-naphthalenediol to produce a strong yellow fluorescent compound (AFC). The molecular formula (C 18 H 15 NO4) and structure of the synthesized AFC were determined by nuclear magnetic resonance mass spectrometry characterization, and based on the fact that ALP can induce the hydrolysis of p-aminophenylethyl phenyl phosphate (PAPP) to produce the intermediate tyramine, and TYR can catalyze the production of DA, which in turn activates HRP, a three-enzyme fluorescence immune system was constructed. The high-sensitivity detection of cardiac troponin I was achieved using this system. This advanced signal transduction scheme can provide a new method for studying multi-enzyme cascade systems and constructing multifunctional biosensors.

The present invention belongs to the field of medical immunology. In particular, it relates to the field of detecting glycated hemoglobin (HbA1c) using a time-resolved fluorescence immunosensor. The method includes the following steps: synthesis of a functionalized Eu<supgt;3+< / supgt; chelate; optimization of the enhancement solution; establishment of an HbA1c standard curve; application of the sensor. The present invention intends to utilize the specific recognition of the glycosylated part of glycated hemoglobin by boric acid to synthesize a functionalized Eu<supgt;3+< / supgt> chelate that can specifically recognize glycated hemoglobin. At the same time, by means of the highly specific affinity of antigen-antibody, a time-resolved fluorescence immunosensor with high sensitivity, strong specificity, convenience, and rapidity is constructed to provide a new method for the quantitative detection of glycated hemoglobin.

The utility model discloses a reaction device for heavy metal fluorescence immunoassay and a heavy metal detection system, and relates to the technical field of heavy metal detection, and the reaction device comprises a shell, a sample loading port, a probe inlet, a cleaning port and a liquid outlet. An inner cavity of the shell is used for enriching a functional solid-phase carrier of heavy metal ions, and at least part of the wall surface of the shell is made of a transparent material to form an optical detection window; the sample loading port is formed in the shell and is used for introducing a liquid sample to be detected into the inner cavity; the probe inlet is formed in the shell and is used for introducing a fluorescent probe coupled with a heavy metal specific antibody into the inner cavity; the cleaning opening is formed in the shell and used for introducing cleaning liquid into the inner cavity; the liquid outlet is formed in the shell and is used for discharging reacted liquid. The scheme provided by the utility model is simple to operate and low in cost.

The application discloses a kind of simultaneously identifying wild type (W-MPT64) and 63bp deletion mutant (D-MPT64) Mycobacterium tuberculosis secreted protein 64 monoclonal antibody.The antibody heavy chain and light chain variable region amino acid sequence as shown in SEQ ID NO.1 and SEQ ID NO.5.The Mycobacterium tuberculosis MPT64 double antibody sandwich enzyme-linked immunosorbent assay and fluorescence immunoassay method established using the monoclonal antibody reach 1.5625 pg / mL, significantly higher than the minimum detection limit of prior art, can be used for qualitative and quantitative detection of Mycobacterium tuberculosis secreted protein 64.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

The fluorescent quantitative analyzer convenient to clean comprises a fluorescent quantitative analyzer body and a discharging piece, a discharging bin is horizontally formed in the front end face of the fluorescent quantitative analyzer body, a sliding groove is transversely formed in the bottom face in the discharging bin, the discharging piece comprises a liquid falling box, the liquid falling box is horizontally inserted into the discharging bin, and the sliding groove is formed in the bottom face in the discharging bin. A sliding frame is horizontally fixed to the bottom face of the liquid falling box, the sliding frame is horizontally assembled in a sliding groove of the discharging bin in a sliding mode, a rubber box is vertically installed in the liquid falling box in an inserted mode, an absorbent cotton box is vertically installed in the rubber box in an inserted mode, and a supporting piece is vertically arranged on the top face of the liquid falling box. The problem that the interior of the fluorescence immunoassay quantitative analyzer is polluted due to the fact that the sample diluent in a test tube overflows and drips when the sample diluent is poured and overflows or the detection frame is pushed due to inertial influence caused by inclined grasping when a sample is taken and placed on the detection frame in the later period is solved.

The invention belongs to the technical field of clinical medical examination, and relates to chromatographic test paper for combined detection of primary liver cancer markers and a preparation method, and the chromatographic test paper comprises a sample pad, a fluorescent microsphere combination pad, a nitrocellulose membrane and absorbent paper which are sequentially pasted on a bottom plate in the horizontal direction with overlapped parts; the fluorescent microsphere combination pad contains a fluorescent microsphere labeling mixed solution containing an AFP detection antibody and an FER detection antibody; and the nitrocellulose membrane is coated with a detection line of an FER capture antibody, a detection line of an AFP capture antibody and a quality control line of goat anti-mouse IgG. According to the chromatographic test paper, low-background and high-sensitivity near-infrared fluorescence is used as a detection signal, sensitive, accurate and rapid detection of a fluorescence immunochromatography platform is achieved, meanwhile, synchronous quantitative analysis of the primary liver cancer specific markers AFP and FER is achieved through one-time sampling and one-time sample adding, and therefore the precise diagnosis efficiency of the primary liver cancer is further improved; the patient examination cost is reduced.

The utility model discloses portable food safety comprehensive detection equipment which comprises an upper box body cover and a lower box body which are hinged, a display screen and a main control board are arranged in the upper box body cover, and a lower panel, a printer module, an interface circuit board, a battery and a plurality of detection modules are arranged in the lower box body; the main control board interacts with the detection module and the printer module through the interface circuit board, and the detection module at least comprises a colorimetric spectroscopic detection module, a colloidal gold photographing module and a fluorescence scanning module. The comprehensive food safety rapid detection equipment adopts a portable structure, integrates a plurality of detection modules such as the main control board, the large-size display screen, the printer, the colorimetric spectroscopic detection module, the colloidal gold photographing module and the fluorescence immunoassay scanning module through reasonable space distribution, has relatively comprehensive detection capability, and is convenient to use. And the unified management of various detection data is realized, the requirements of field detection can be met, and the portability of the equipment is improved.

The invention provides a method for reducing non-specific adsorption of a plate type biomarker and application of the method, and belongs to the technical field of biological detection. The carboxylation plate is used for replacing a conventional elisa plate used in a digital fluorescence immunoassay technology, compared with the conventional elisa plate, the carboxylation plate has the advantages that the background of the carboxylation plate is obviously reduced, the carboxylation plate can be used for immobilizing an antibody through covalent bond combination, and the carboxylation plate has the advantages of good stability, excellent repeatability and obviously lower non-specific adsorption; the accuracy of digital fluorescence immunodetection can be improved, the accuracy of clinical related biomarker detection is improved, and technical support is provided for accurate diagnosis of patient diseases.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a reaction cup transfer device which comprises a mounting frame, an incubation disc and a cleaning disc which are rotationally arranged on the mounting frame, and an incubation lapping seat and a cleaning lapping seat which are respectively arranged at the lower ends of the incubation disc and the cleaning disc in an annular array, the stirring finger mechanism is arranged on the mounting frame and is positioned below the incubation disc and the cleaning disc; the shifting finger mechanism comprises a fixing frame, an overturning motor arranged on the fixing frame, an overturning shaft rotationally arranged on the fixing frame, an overturning sleeve arranged on the overturning shaft in a sleeving mode, a finger plate fixed to the overturning sleeve, a translation motor arranged on one side of the fixing frame and a driven belt wheel arranged on the other side of the fixing frame. The translation belt is arranged at the driving end of the translation motor and on the driven belt wheel, and the translation seat is connected with the translation belt. According to the design, the reaction cup can be stably pushed and transferred, the structure is simple, cost is low, and operation and control are convenient.