Time resolution fluorescence immunoassay method and kit for fast detecting gutter oil

A time-resolved fluorescence, waste oil technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of poor specificity, prone to false positives, and low accuracy of waste oil detection methods, and achieves short detection time, The effect of high sensitivity and wide detection range

Inactive Publication Date: 2015-08-19
GUANGZHOU CENT FOR DISEASE CONTROL & PREVENTION (GUANGZHOU HYGIENE INSPECTION CENT GUANGZHOU CENT FOR FOOD SAFETY RISK SURVEILLANCE & ASSESSMENT INST OF PUBLIC HEALTH OF GUANGZHOU MEDICAL UNIV)
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Problems solved by technology

However, thin layer chromatography is prone to false positives for some unpurified oils (such as sesame oil, pepper oil, etc.) and oils with other ingredients (such as chili oil)
[0008] In summary, the existing methods are not very accurate and specific
On October 12, 2011, the spokesperson of the Ministry of Health of my country, Deng Haihua, revealed that the 5 detection methods for gutter oil collected so far are not very specific, and the relevant departments will open the collection methods to the public again.

Method used

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  • Time resolution fluorescence immunoassay method and kit for fast detecting gutter oil
  • Time resolution fluorescence immunoassay method and kit for fast detecting gutter oil
  • Time resolution fluorescence immunoassay method and kit for fast detecting gutter oil

Examples

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Embodiment 2

[0101] Example 2 Detection of commercially available low-grade edible oil samples

[0102] First process the sample: add 5ml of waste oil sample into the test tube, then add 2ml of distilled water, shake vigorously, and take 1ml of water for later use when the water and oil are separated.

[0103] Take the LPS-BSA strip, add 50 μL of LPS standard or processed samples to the respective microwells, add 50 μL of LPS monoclonal antibody diluted 1:20 in buffer, shake at 25°C for 0.5 hours, wash with washing solution 3 times, Add 100 μL of Eu3+-goat anti-mouse antibody diluted with buffer 1:20, shake at 25°C for 1 hour, wash with washing solution 6 times, add 200 μL of enhancement solution and shake for 5 minutes before measuring. Calculate the FB1 content in the sample from the standard curve, see Table 1, the sample concentration in this example is 31.7ng / mL (5241ng / g). see table 3

[0104] table 3

[0105]

[0106] The time-resolved immunological detection method of the pre...

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Abstract

The invention discloses a time resolution fluorescence immunoassay method and kit for fast detecting gutter oil. The method starts with the germ types massively propagated in the gutter oil, and advantageous germs polluting the gutter oil are analyzed, and specific components of the advantageous germs are determined to achieve the aim of identifying the gutter oil; the research method is very unique; lipopolysaccharide of the advantageous germs in the gutter oil is used for screening monoclonal antibodies and a TRFIA detection method of the lipopolysaccharide is established. The method has the advantages of high specificity and high detection sensitivity for the gutter oil, and has very important significance in identification and detection of the gutter oil.

Description

technical field [0001] The invention relates to the fields of food safety and biochemical monitoring, in particular to a time-resolved fluorescent immunological method and a kit for rapidly detecting waste oil. Background technique [0002] Waste oil is a non-edible oil with extremely poor quality and hygiene, and indicators such as peroxide value, acid value, moisture, carbonyl value, malondialdehyde, and AFBI (Aflatoxin Bl (AFBI)) seriously exceed the standard. Waste oil from the catering industry contains a large amount of toxic and harmful substances. After human consumption, it will cause poisoning symptoms such as dizziness, nausea, vomiting, diarrhea, insomnia, fatigue, indigestion, liver discomfort and severe abdominal cramps. Long-term consumption will cause anemia, Malnutrition and damage to internal organs can lead to weight loss and developmental disorders in children. In severe cases, toxic liver disease may occur, which can induce gastric adenocarcinoma, kidney...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 李军涛陈守义朱伟杨智聪
Owner GUANGZHOU CENT FOR DISEASE CONTROL & PREVENTION (GUANGZHOU HYGIENE INSPECTION CENT GUANGZHOU CENT FOR FOOD SAFETY RISK SURVEILLANCE & ASSESSMENT INST OF PUBLIC HEALTH OF GUANGZHOU MEDICAL UNIV)
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