7 results about "Time resolved fluorescence immunoassay" patented technology

The invention discloses a time-resolved fluorescence immunoassay analyzer, comprising a base, a sample injection module, an incubation module, a cleaning module and a chip detection module; wherein a chip loading module and a liquid-gas circuit module are arranged in the sample injection module, the chip loading module is used to carry the chip to the liquid-gas circuit module, the liquid-gas circuit module is used to make the sample in the chip flow to a predetermined position; the chip is then transported to the incubation module; the incubation module allows the chip to react at a constant temperature within a set temperature; the incubation module moves freely up and down in a vertical direction, the sample injection module, the cleaning module and the chip detection module are respectively arranged on the side of the vertical movement direction of the incubation module; the incubation module sends the chip cleaned in the cleaning module to the chip detection module for detection. The modules are arranged in a vertical direction, the overall structure is compact, and the floor space is small; and the functions of the modules are coordinated smoothly and simply, and the detection efficiency is high.

The invention discloses a time-resolved microsphere and a preparation method thereof. The preparation method comprises the following steps: S1, preparing a europium complex; s2, preparing white carboxyl latex microspheres; and S3, preparing the time-resolved microspheres by a swelling method. The time-resolved fluorescent microspheres prepared by the invention have the characteristics of high brightness, ultra-long fluorescence lifetime, excellent stability, good biocompatibility, easiness in functionalization and the like, so that the potential of a time-resolved fluorescence immunoassay technology is fully exerted, and the ever-increasing requirements of high sensitivity, high accuracy and rapid detection are met.

The invention discloses a time-resolved fluorescence immunoassay analyzer, which comprises a base, a sample introduction module, an incubation module, a cleaning module and a chip detection module, wherein a chip loading module and a liquid path and gas path module are arranged in the sample injection module; then, the chip is transferred into the incubation module; the incubation module enables the chip to react at a constant temperature within a set temperature; the incubation module freely moves up and down in the vertical direction, and the sample injection module, the cleaning module and the chip detection module are respectively arranged on the side surfaces of the incubation module in the vertical movement direction; the incubation module sends the cleaned chip in the cleaning module to the chip detection module for detection; the cleaning module comprises a U-shaped shifting handle assembly and a cleaning module gas-liquid path assembly; the chip detection module comprises an optical path detection assembly and a detection shifting handle assembly. The modules are arranged in the vertical direction, the overall structure is compact, and the occupied area is small; the modules are smooth and simple in function cooperation, and the detection efficiency is high.

The invention discloses a nano Eu chelate labeled NGAL antibody AKI detection reagent, and relates to the technical field of biomedical detection. The reagent comprises a nano Eu chelate labeled NGAL specific antibody, an NGAL McAb coated plate, an NGAL standard solution and an auxiliary reagent, wherein the nano Eu chelate is prepared by coupling DSC activated carboxyl with the NGAL specific antibody. The time-resolved fluorescence immunoassay technology is adopted, the kit has the advantages of being high in sensitivity, good in stability and rapid in detection, the NGAL concentration in a sample can be accurately detected, index changes can be detected in the early stage after AKI happens, meanwhile, the kit is suitable for monitoring the progress of CKD in all stages, reliable technical support is provided for early diagnosis and illness state evaluation of kidney injury related diseases, and the kit is worthy of popularization and application. The important clinical application value is realized.

The invention relates to a hybridoma cell strain secreting a vitamin B12 monoclonal antibody and application of the hybridoma cell strain. A monoclonal antibody with high sensitivity and specificity to the vitamin B12 is screened out, and the time-resolved fluorescence immunoassay kit for detecting the vitamin B12 is prepared by combining a time-resolved fluorescence analysis technology. The time-resolved fluorescence immunoassay kit disclosed by the invention has high sensitivity to vitamin B12, can detect 0.05 mu g / kg of vitamin B12 in milk powder, and is far lower than the detection limit of 4 mu g / kg in the prior art. And the time-resolved fluorescence immunoassay kit disclosed by the invention has very high specificity on the vitamin B12, and has no inhibition effect on VB3, VB9, VB5 and VB6. Therefore, the time-resolved fluorescence immunoassay kit disclosed by the invention can be used for accurately detecting the vitamin B12, and is particularly widely applied to the detection of special medical foods.

The application discloses a 21-hydroxylase deficiency screening kit and application thereof, relates to the blood analysis technical field, and a steroid hormone marker composition of 21-hydroxylase deficiency, wherein the nine markers comprise 17alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol, corticosterone, progesterone, dihydrotestosterone and 11-deoxycorticosterone; the kit can be used for screening 21-hydroxylase deficiency, 11beta-hydroxylase deficiency, 17alpha-hydroxylase deficiency and other types of diseases; the kit comprises detection components for detecting the hormones; compared with a traditional time-resolved fluorescence immunoassay method, the LC-MS / MS multi-index combined detection scheme adopted in the application can significantly reduce false positive results; and invalid recall, unnecessary family anxiety and medical burden caused by false positive results are greatly reduced.

The invention provides a group of specific antibodies for detecting a renal injury molecule 1 and application thereof. The antibody comprises mouse monoclonal antibodies B0011 and B0035. The invention discloses variable regions and CDR sequences of a heavy chain and a light chain of the antibody. Experiments prove that the antibody has the characteristics of high affinity and strong specificity. A time-resolved fluorescence immunoassay (TRFIA) kit is established on the basis that the antibody B0011 serves as a capture antibody and the antibody B0035 serves as a detection antibody, the detection sensitivity of the kit can reach 5 pg / mL, the linear range is 5-5000 pg / mL, and the kit has no cross reaction with other renal injury markers. The kit can stably and accurately monitor the level of trace KIM-1 in blood, and provides a powerful tool for early diagnosis, curative effect monitoring and prognosis of renal tubular interstitial injury.