46 results about "Antibody antigen reactions" patented technology

The invention belongs to the technical field of biology and relates to a protein chip reagent kit and a method for comprehensively detecting a lung cancer marker. The protein chip reagent kit for comprehensively detecting the lung cancer marker comprises: (1) a chip (1) and (2) a reaction agent and a detection agent, wherein a plurality of specific antibodies are simultaneously fixed on the chip, can generate antibody-antigen reaction with the lung cancer marker and are fixed on a bottom film of the chip to form a plurality of independent recognition sites; and the reaction agent and the detection agent are used for detecting whether a matter capable of generating antibody-antigen reaction with the specific antibodies exists in a sample to be detected or not through a TRFIA (Time Resolved Fluorescence Immunoassay) method. The reagent kit and the method have the beneficial effects that the six indexes of NSE (Neuron Specific Enolase), SCC (Squamous Cell Carcinoma), CEA (Carcino Embryonie Antigen), CA (Carbonic Anhydrase) 19-9, CYFR (Cytokeratin Fragment) A21-1 and pro-GRP (Glass Reinforced Plastic) can be simultaneously detected, the detection accuracy is improved, the repetitive experimental steps are reduced, and the time and the cost are saved.

The invention discloses an optical waveguide immunosensor and a detection method thereof. The optical waveguide immunosensor basically comprises an optical waveguide resonator cavity, a magnetic needle and an immunologic reaction tank. The method comprises the following steps of: carrying out surface specificity modification to nano magnetic particles; capturing a substance to be detected by utilizing an antibody-antigen reaction; orderly limiting the nano magnetic particles on the surface of the circular or disc-shaped optical waveguide resonator cavity by utilizing the magnetic needle; sensing refraction index change before and after an immunologic reaction by utilizing an evanescent field; and analyzing corresponding resonance spectrum change to acquire the content of the substance to be detected in a sample. The optical waveguide immunosensor has simple structure. Based on the circular or disc-shaped optical waveguide resonator cavity with compact structure, the nano particles after immunologic specificity modification are absorbed to the optical waveguide surface by the magnetic needle to measure the offset of a resonance frequency and accurately and sensitively detect the immunologic reaction, and the device can be repeatedly used so that the invention has favorable application prospects.

There is provided a polydiacetylene supramolecule comprising diacetylene molecules, capable of immobilizing a receptor molecule having a thiol group. Since the polydiacetylene supramolecule has a receptor immobilized thereon having a thiol group, for example, an antibody, and thus shows color transition when reacting with a sample, an antigen can be detected through specific color transition of the polydiacetylene when employing in a receptor-ligand reaction, for example, an antibody-antigen reaction.

The microfluid control chip for immunological analysis features that the chip consists of four basic units connected successively, with the sample outlet of the upper basic unit being connected to the sample inlet of the next basic unit. The first basic unit is sample injecting unit; the second one is antibody and antigen reaction unit with specific antibody linked to the inner wall of the micro channel loaded on the stuffing; the third one is post-column fluorescent mark unit; and the fourth one is antigen separating and detecting unit. The chip of the present invention is used in analysis of target antigen to obtain information in short time and through simple operation.

The invention provides a full-automatic hybridization oven and an implementation method and application thereof. The full-automatic hybridization oven structurally comprises a hybridization reacting box, a sample feeding device for adding a reagent to the hybridization reacting box, a reagent supply device, a reagent temperature controlling device and a control terminal, wherein, the hybridization reacting box is provided with hybridization membrane strips, the reagent supply device is sequentially connected with the reagent temperature controlling device and the sample feeding device througha pipeline, and the control terminal is connected with the reagent temperature controlling device. The hybridization process of the hybridization oven of the invention is controlled by the control terminal to realize full-automatic hybridization operation. The invention can be applied to existence and quantification of affinity mates in chemical molecular binding reactions such as nucleic acid hybridization, antibody-antigen reaction, receptor-ligand reaction, ion-culture medium reaction in a chemical binder and the like.

The invention discloses a kit for detecting phosphorylated epidermal growth factor receptor and the kit adopts the protein chip technology. The kit comprises a base film (1) fixed with several kinds of specific antibodies and reactant and detection agent (2), wherein specific antibodies and phosphorylated epidermal growth factor receptor can perform antibody-antigen reaction, each kind of specific antibodies are fixed on a plurality of independent recognition sites which are fixed on the base film; and the reactant and detection agent (2) are used to detect whether the substance which can perform antibody-antigen reaction with specific antibodies exists in a sample to be detected through the array-ELISA method. The invention also discloses a preparation method of the kit. By adopting the kit of the invention, 17 phosphorylated epidermal growth factor receptors in a plurality of samples can be detected, lots of defects of the prior art can be overcome and the kit has the advantages that the kit is cheap, convenient, sensitive and accurate, has high flux and less amount of sample and can be popularized in the ordinary laboratory and scaled, etc.

A biosensor capable of detecting, for example, the index of refraction, the concentration of proteins, or antibody-antigen reactions in a sample using surface plasmon resonance are provided, as well as methods for producing the biosensor sensor measurement systems using the biosensor. A biosensor may comprise transparent rod 2; metallic reflector 40 formed on end surface 2a of one end of transparent rod 2; metallic thin film 3 formed on the outer circumferential surface of said one end of transparent rod 2; and organic substance layer 4 comprising a photo immobilizing agent containing a photo cross linking agent and a substance to be immobilized which is formed on the metallic thin film 3 on the outer circumferential surface and immobilized.

The invention relates to the field of molecular biology, particularly a kit for separating RNA (ribonucleic acid) bound in an RNA binding protein. The kit provided by the invention is used for separating, extracting and purifying RNA bound in a specific RNA binding protein. The kit comprises a lysis solution, a buffer solution and magnetic beads. The reagents in the kit are proportionally improved and optimized, and thus, can simply and stably obtain the mRNA (messenger ribonucleic acid) bound in the specific RNA binding protein in cells at higher yield through antibody antigen reaction and magnetic field principle. The kit is used for further cDNA real-time quantification, micro-array chip detection and other subsequent researches.

The invention discloses a kit for diethyl phthalate fluorescence polarization immunoassay. The kit comprises a fluorescence indicator stock solution, a DEP (Diethyl Phthalate) standard solution, a DEP polyclonal antibody serum and a concentrated phosphate buffer solution (PBS), which are respectively encapsulated in a reagent tube for detecting the concentration of the DEP in a sample. According to the detection method, a fluorescence polarization technology is adopted, a standard curve is obtained through measuring the fluorescence polarization of a series of DEP solutions with the known concentrations, and the concentration of the DEP in a mixture is computed by using the standard curve. The kit, provided by the invention, has the advantages that high degree of specificity of an antibody-antigen reaction and high sensibility of a fluorescence indicator are combined by the detection method, so that the kit is simple and quick to operate, free of operations such as separation during detecting, and high in detection precision, the maximum detection range of the DEP is 10 to 200 ng/mL, the sensitivity reaches 40.4 ng/mL, and the kit plays an important role in quick detection of the residual DEP in actual systems, such as water, juice drinks and capsules.

The invention relates to a filtration recombiner, in particular to a parallel circulation cell selective filtration recombiner. The parallel circulation cell selective filtration recombiner adopts the technical proposal that the parallel circulation cell selective filtration recombiner comprises an injection pump, a first pipe, a second pipe, a first right connecting pipe, a first left connecting pipe, a second right connecting pipe, a second left connecting pipe, a first bidirectional crack valve, a second bidirectional crack valve, a switch valve, an exhaust hole and a peristaltic pump, and also comprises a filtration box which is provided with a filter screen and a movable cover. The parallel circulation cell selective filtration recombiner has the advantages of needlessness of antibody and antigen reaction, needlessness of using beads and other media, needlessness of in vitro culture, needlessness of special centrifugal equipment, quick speed, and utilization of the adhesivity of bone marrow stromal cells to perform bone marrow stromal cell filter-type quick screening on marrow blood.

The invention discloses an electrophoresis flowing type ELISA method based on a PEM substrate, belonging to the field of biomaterials. The method comprises the following steps: allowing a first antibody to be adsorbed on the surface of a porous membrane and then carrying out blocking agent protein adsorption; carrying out antigen-first antibody reaction under the driving of electrophoresis; carrying out second antibody-antigen reaction and carrying out color development and quantification on the enzyme-labeled second antibody, i.e., after the antigen-first antibody reaction under the driving of electrophoresis, placing the porous membrane substrate having undergone electrophoresis in a second antibody solution, carrying out a reaction, then carrying out cleaning, placing the obtained porous membrane substrate in a substrate solution of second antibody identification enzyme and carrying out color development; and carrying out quantitative analysis on antigen by detecting absorbance. Preferably, an electropositive PDDA/PSS modified cellulose acetate membrane PEMs-CA is used. According to the invention, through modification of PEMs, non-specific adsorption is reduced; electrophoresis driving force enables antigen molecules to be locally concentrated around the first antibody in a short period of time; so rapid and sensitive detection is realized.

A silicon biosensor and a manufacturing method thereof is provided, the silicon biosensor includes: a light source performing self emission a light detector generating a photoelectric current corresponding to an amount of incident light an optical fiber transmitting the light from the light source to the light detector and a micro fluidic channel adjusting an optical transmission rate of the optical fiber according to an antibody-antigen reaction when the antibody-antigen reaction occurs. The silicon biosensor can be easily integrated or bonded with a silicon electronic device, so that it is possible to manufacture the biosensor with a low price, under mass production.

The present invention is based on rapid displacement of solvents under mild vacuum in solution-solid phase reaction of immunocomplex [Ijsselmuiden et al., J. Immunol. Methods, 6 (1989): 35]. In the present invention, this is achieved by a microporous absorbing pad upon which a nitrocellulose transfer membrane is placed. The absorbing pad under mild vacuum generated/regulated by running tap effectively filters out the unbound ligand and rinsing solutions through transfer membrane, thus enhancing the reaction kinetics of immunocomplex. This mechanism, in turn reduces the incubation steps of antibody-antigen reaction from hours to few minutes, allowing total assay time in less than 20 minutes.

The invention relates to an HLA antibody specificity detecting method, a cell dish and a reagent kit, belonging to the field of immunity detection. The HLA antibody specificity detecting method comprises the following steps: (1) enabling serum to be detected to respectively undergo antibody antigen reaction with peripheral blood lymphocytes taken from different donors; (2) detecting the result of the antibody antigen reaction; and (3) processing data to obtain the HLA antibody specificity. The HLA antibody specificity detecting method is characterized in that the HLA antigen frequency is adjusted by selecting the taken donors so that the detected PRA value is lower than the PRA value of the serum to be detected, and the limitation to the HLA antibody specificity detection caused by the HLA antigen overlapping phenomenon is reduced. The method can be used for detecting the HLA antibody specificity of a serum with higher PRA value and the HLA antibody specificity of the serum to be detected once so that the HLA antibody specificity detecting method is stepped forwards in a leap way and brings benefit to people.

The invention relates to an antibody modified nanoparticle electrophoresis flowing type ELISA (enzyme linked immunosorbent assay) method and belongs to the field of biological materials. The method is characterized in that a first antibody attaches to the surface of a porous film, and sealant protein absorption is performed; antigen-first antibody reaction is performed; electrophoresis is used to drive the antibody to modified nanoparticles to perform second antibody-antigen reaction, and developing quantification is performed on an enzyme-labeled second antibody; the porous film is cleaned after the electrophoresis is used to drive the second antibody-antigen reaction, and the obtained porous film substrate is placed into the substrate solution of second antibody labeling enzyme for developing; antigen quantitative analysis is performed by detecting light absorbency. The method has the advantages that positive PDDA/PSS is used to modify a cellulose acetate membrane PEMs-CA, and carboxyl polystyrene nanoparticles are used as the carriers modified by the enzyme-labelled second antibody; PEMs modification lowers nonspecific absorption, antibody modified nanoparticles can amplify signals, electrophoresis drive force allows the second antibody to locally concentrates to the periphery of the antigen in a short time, and fast and sensitive detection is achieved.

The invention provides a melamine quick detection reagent which adopts highly specific antibody and antigen reaction and immunity-chromatographic technology to achieve the aim of quickly detecting the melamine in a sample. The reagent has the advantages of simple, convenient and quick operation and the like, can be applied to the production fields such as bread cultivation, breeding, animal husbandry, food processing and the like for quickly detecting the melamine in the food and feed in-situ and also provides simple and convenient means for the safety detection of household food.

A noncontact stirring method, a noncontact stirring apparatus, a method and apparatus for reacting nucleic acid hybridization using the apparatus, a method for detecting nucleic acid in a sample, an apparatus for detecting nucleic acid, a method for detecting antibodies in a sample, and an apparatus for detecting antibodies in minute droplets 1 μmL or less in an electric field are proposed that can be utilized to speed up biodetection processes, hybridization processes in DNA analysis, ELISA antigen fixing processes, blocking processes, antibody-antigen reaction processes and color reaction processes; and pathogen identification, CRP tests, methods of propagating cells or bacilli such as colon bacilli in a liquid culture medium, and chemical analyses.

The invention discloses a detection kit for a free human chorionic gonadotropin beta subunit. The detection kit comprises a biotin-labeled free human chorionic gonadotropin beta subunit antibody, a streptavidin magnetic particle suspension, an acridinium ester-labeled free human chorionic gonadotropin beta subunit antibody, a standard substance, a cleaning solution, a diluent, a chemiluminescent substrate solution A and a chemiluminescent substrate solution B. The streptavidin and the biotin have high-specificity binding capacity, streptavidin and biotin-labeled high-purity antibody non-covalent bonds are specifically bound, the cascade amplification effect is achieved and the reaction is highly specific. The high specificity of antibody-antigen reaction and high sensitivity of acridiniumester luminescence are combined, photons generated by reaction are captured by acridinium ester to detect the concentration of the product, and the detection kit has the characteristics of higher sensitivity, short reaction time, simple operation and high anti-interference performance.