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Prostateexosomal protein antigen, antibody and application thereof

A prostatic corpuscle and protein antigen technology, which is applied to the prostatic corpuscle exocytosis protein antigen and the preparation field thereof, can solve the problems that the detection of prostatic inflammation is not suitable, and the samples of patients with chronic prostatitis and normal people cannot be well differentiated, and the detection is achieved. Simple and reliable method

Active Publication Date: 2015-09-09
ONCO BIOMEDICAL TECH SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this kit is only suitable for the detection of prostate cancer and cannot distinguish between samples from patients with chronic prostatitis and normal people, so it is not suitable for the detection of prostatitis

Method used

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  • Prostateexosomal protein antigen, antibody and application thereof
  • Prostateexosomal protein antigen, antibody and application thereof
  • Prostateexosomal protein antigen, antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of prostatic exudate protein antigen (PSEP): Take 500 ml of mid-morning urine from patients with chronic prostatitis, add an equal amount of HEPES buffer (pH7.3) and protease inhibitor (Sigma, USA), and filter with gauze. The filtrate was taken, placed in ten 50ml centrifuge tubes, and centrifuged at 400g for 10 minutes at 4°C. Remove the precipitate and take the supernatant. Centrifuge at 10,000g for 20 minutes at 4°C. Remove the precipitate and take the supernatant. Place them in 200 3ml ultracentrifuge tubes and centrifuge at 100,000g for 120 minutes at 4°C. Pour off the supernatant and take the precipitate. Add 2.5 ml of TBS buffer (pH7.8) and protease inhibitors (Sigma, USA), and mix well. Centrifuge at 100,000g for 120 minutes at 4°C. Pour off the supernatant and take the precipitate. They were dissolved in 2.5 ml of 0.5% NP-40, 80mMNaCl, 0.2% Deoxycholate, 50mM Tris, pH8.0 and protease inhibitors (Sigma, USA). Centrifuge at 100,000g for 20 minu...

Embodiment 2

[0046] The composition of the kit described in this embodiment is as follows: mid-section urine of a patient with prostatitis; blocking solution; prostatic exosomal protein antigen (PSEP); PSEP detection antibody; cleaning solution; chromogen; stop solution.

[0047] The specific method for detecting or diagnosing prostatitis using the above kit is as follows:

[0048] 1. Source of specimens: According to the diagnostic criteria for prostatitis in the "Guidelines for the Diagnosis and Treatment of Urological Diseases in China" (Na Yanqun et al., 2011), 213 samples of mid-section urine from patients with prostatitis were collected, and chronic prostatitis patients who fully met the clinical comprehensive diagnosis were selected. 176 cases of prostatitis. Aged 16-72 years old, average age 34 years old. 100 samples of urine from normal people, aged 20-68 years. The average age is 34 years old. Urine routine were normal. Test the urine immediately after collection, or store it...

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Abstract

The invention relates to a prostateexosomal protein (PESP) antigen, a specific corresponding antibody and application thereof, and on the basis further acquires a kit based on prostasome leakage protein quantitative indirect enzyme-linked immunoadsorption so as to effectively detect and diagnose chronic prostatitis. By means of reaction system precision detection, stability test and destructive test on the urine detection kit based on PESP antibody-antigen reaction, the reagent quality shows high batch production probability. The application is truly noninvasive, the operation is simple, and the kit has high sensitivity and specificity. The kit can alleviate the patient pain caused by chronic pelvic pain syndrome / chronic prostatitis, and also early diagnosis can further strengthen prevention and treatment of prostate cancer, therefore the kit has wide application prospect.

Description

technical field [0001] The invention relates to a prostatic corpuscle leaking protein antigen, in particular to a prostatic corpuscle leaking protein antigen and a preparation method and application thereof. Background technique [0002] Chronic prostatitis (CP) is one of the most common diseases in young and middle-aged men in urology department, and it is reported that it accounts for 30% of outpatient visits. According to epidemiological surveys, more than 10% of adult males in my country have prostatitis-like symptoms, and 50% of males have suffered from prostatitis in different periods. It shows that there are many patients with potential chronic prostatitis or chronic pelvic pain (CPPS). At the same time, the inflammatory process caused by chemicals, physical factors or biological agents is also an important factor in the pathogenesis of human cancer. In prostate cancer, the presence of inflammation and regeneration of cellular epithelial tissue after injury is consi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/577G01N33/68
Inventor 钱泽陆群曾燕张娇
Owner ONCO BIOMEDICAL TECH SUZHOU
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