74 results about "Ultracentrifuge" patented technology

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

The invention discloses an ultra centrifuge. The ultra centrifuge solves the two major problems of development of high-speed motors and development of high-speed vacuum sealing devices, air floatation and air floatation hybrid bearing technology is adopted in the design of the high-speed motors, and bearing stiffiness and bearing stability are enhanced greatly; a large interference shrink fit method is adopted in the design of protective sleeves, and completeness of rotors is protected effectively; the rotors are of a three-section structure; a stator core is of a relatively eclectic 12-groove structure, and a winding utilizes a ring-type winding; and the cooling method is a force-air cooling method, and reliable and stable operation of an ultra centrifuge driving device is guaranteed. High-speed vacuum sealing utilizes a magnetic drive vacuum dynamic sealing connection method, and non-contact transmission of motive power is achieved through magnetic transmission between an inner magnet rotor and an outer magnet rotor. The successful development of the ultra centrifuge changes the passive situation that out country completely relies on import in an ultra centrifuge market, and the ultra centrifuge has wide market prospects and great social benefits.

The invention relates to a method for preparing monodisperse polymer microspheres. The method comprises the following process steps: adding a dispersion medium with a stabilizing agent dissolved inside to an impinging stream reactor, heating the dispersion medium, adding a monomer with an initiating agent dissolved inside in one step, carrying out constant-temperature polymerization reaction, obtaining polymer microsphere emulsion after stopping cooling, then using an ultracentrifuge to carry out centrifugal sedimentation, adding absolute ethyl alcohol after removing supernatant, carrying out ultrasonic washing multiple times and drying the washed microspheres to constant weight, thus obtaining the monodisperse polymer microspheres. Compared with the prior art, the method has the following advantages: the prepared polymer microspheres have good apparent morphology, good smoothness and monodispersity and uniform grain sizes; the process is simple to operate; the grain sizes of the microspheres can be well controlled according to the formula; and the grain size range applicability is better.

The invention discloses a method for extracting and separating extracellular secretions. The method comprises the following operation steps of placing target cells into a selected culture medium, obtaining the target cells 72 hours after cell culture, and conducting centrifugation to obtain a cell culture medium supernatant; centrifuging the obtained cell culture medium supernatant through a low-temperature centrifuge at 4 DEG C and 6,000 xg for 30 minutes, transferring the supernatant in a centrifuge tube to a new tube for later use after the centrifugation is finished, and discarding the precipitate; sucking the supernatant through a disposable syringe, and slowly dripping the supernatant to form a cell filter membrane with the specification of 0.22 microns for filtration; placing the cell culture medium into an ultracentrifuge to carry out ultra-speed centrifugation at 4 DEG C and 100,000 xg for 70 minutes, removing a supernatant, and keeping a precipitated part after the centrifugation is finished; washing and re-suspending the precipitate through a PBS buffer solution, putting the mixed liquid into the ultracentrifuge again to carry out ultra-speed centrifugation at 4 DEG C and 100,000 xg for 70 minutes, carefully sucking out a supernatant and keeping a precipitated part after the centrifugation is finished; using the PBS buffer solution for resuspending and precipitationto obtain a precipitate, namely an extracted and separated exosome product.

The invention relates to an apple polyphenol extracting method, in particular to an apple polyphenol preparation method. The invention solves the problems of low extraction rate, poor activity and complex production process of the traditional apple polyphenol extracting method. The apple polyphenol extracting method comprises the following steps: selecting apples, crushing, pulping, extracting by a highfield, separating extract liquor by an ultracentrifuge, removing residues, concentrating by a vacuum wiped thin film, drying by vacuum freeze drying equipment to obtain freeze dried powder of apple polyphenol, and respectively adding blueberry anthocyanin, vitamin C and seabuckthorn flavone to prepare a nutrition enhancer. The apple polyphenol prepared by the invention is a natural extract without side effects on a human body, has functions of strengthening the immunity, resisting tumors, reducing blood fat, resisting coagulation and thrombus, reducing the weight, beautifying the skin and the like, and can be applied to the fields of foods, health care products, cosmetics, medicines, biochemical industry and the like.

The invention provides an integrated detection method for separating, enriching and detecting an exosome. The method comprises the following steps: designing and assembling a dual-membrane filtration chip, constructing an ELISA detection standard curve, collecting a sample, injecting and washing, performing chip ELISA detection, and performing data processing analysis. The invention also provides an integrated detection chip for separating, enriching and detecting a urine exosome. A reaction system and the detection method of the present invention can be used for detecting the content of a urine sample of a bladder cancer patient and the exosome in a supernatant of a bladder cancer cell line nutrient solution. The detection chip has characteristic of high specificity, generates no influence or wound on human body, and the detection method does not require the expensive precision experiment apparatus (such as an ultra centrifuge and a fluorescence microscope), and has large application prospect.

The invention belongs to the field of biomedicine and particularly discloses a method for preparing monodisperse polystyrene microspheres with a controllable particle size. The method is characterized by comprising the following steps of: adding water and emulsifier into a three-necked flask, stirring a solution until the solution is clarified, introducing nitrogen to replace air in the reaction system, adding a certain amount of monomers, emulsifying for 0.5 to 2.5 hours, heating to reaction temperature of between 65 and 90 DEG C, stirring again, dripping an initiating agent under the protection of nitrogen, and performing polymerization reaction for 12 to 48 hours; and stopping reaction to obtain emulsion particles, performing centrifugal sedimentation by using an ultracentrifuge, discarding supernate, adding absolute ethanol, performing ultrasonic dispersion, repeating the steps for 3 to 4 times, pouring washed microspheres into culture dish, putting in a vacuum drying oven, and drying to obtain polymer microspheres. Compared with the prior art, the method for preparing the monodisperse polystyrene microspheres with the controllable particle size has the advantages that raw materials are low in cost and can be obtained easily; the process is simple; and the prepared monodisperse polystyrene microspheres with the controllable particle size can be applied to the field of biomedicine, micro electronic techniques and the like well.

The invention discloses a method for extracting platycodon root polysaccharide, which relates to a method for preparing the platycodon root polysaccharide and solves the problems of low yield, long consumed time, low polysaccharide activity and fussy process in the conventional platycodon root polysaccharide extraction process. The method for extracting the platycodon root polysaccharide comprises the following steps of: selecting dry platycodon root, degreasing the crushed powder, then extracting polysaccharide from the platycodon root by a microwave-aid water extracting method to obtain a polysaccharide extract, removing protein and then separating the extract by using an ultra-speed centrifuge, concentrating the extract by using a film under vacuum, and freeze-drying the extract by using a vacuum freeze dryer after dialysis concentration to obtain platycodon root polysaccharide freeze-drying powder. The platycodon root polysaccharide extracted by the method has the effects of enhancing immunologic function, resisting tumor, reducing blood fat and blood pressure, resisting cruor and thrombus, losing weight, caring skin, promoting the healing of cornea wound, improving dry eyes and the like. The platycodon root polysaccharide can be applied in the fields of food, health-care products, cosmetics, medicaments, biochemical engineering and the like.

A blackfungus polyhexose extraction method relates to a Blackfungus polyhexose preparation method. The invention solves the problems of low extraction rate, low activity and fussy production technique existing in extracting the blackfungus polyhexose with the existing traditional method. The technical steps of the blackfungus polyhexose extraction method are as follows: blackfungus is selected, sliced and cracked, and is extracted by a strong electric field; then a supercentrifuge is used for separating the extraction liquid and removing the protein and lipid materials, and then a vacuum scraper type film is used for concentrating, and a vacuum freezedryer is used for freeze-drying, thus obtaining the freeze-dried powder of the blackfungus polyhexose. The blackfungus polyhexose prepared by the invention is a natural extract, does not have side effects on the human bodies, and has the functions like increasing the immunologic function, resisting knubs, reducing blood-fat and blood pressure, resisting cruor and thrombus, losing weight, caring beauty and skin, and the like. The blackfungus polyhexose can be broadly applied in the fields like food, health products, cosmetics, medicines, biochemical industry, and the like.

The invention discloses a method for adsorbing and separating extracellular vesicles including exosomes secreted to a medium by cells based on an anion exchange resin, the extracellular vesicles including exosomes produced by the method, and application thereof. The method provided by the invention is simple and convenient to operate and only needs to use chromatographic tubes / columns and pipetteguns and other common laboratory consumables, does not require use of ultra centrifuges, saves cost and time; the obtained extracellular vesicles has high purity and large concentration, can be produced in a large scale, and is easy to be further applied clinically.

Disclosed is a method of isolating extracellular vesicles using an aqueous two-phase system (ATPS), including (a) preparing an ATPS by mixing a first material and a second material, which are immiscible with each other, with a body fluid or an aqueous solution containing extracellular vesicles and (b) isolating extracellular vesicles concentrated in the second material of the ATPS. This method can exhibit very high isolation efficiency, a simple isolation manner, and a very short isolation time. The isolation of extracellular vesicles using the ATPS requires no ultracentrifuge and achieves almost 100% isolation efficiency within a short time of about 10˜20 min, and thus the method of the invention is practical, is economical due to low costs thereof, can increase the purity of extracellular vesicles contaminated with protein, enables the diagnosis of disease using the isolated extracellular vesicles, and can be applied to various fields using extracellular vesicles.

This invention provides a method for separating and enriching isotopes in an efficient and low-cost manner from a condensation-system (liquid or / and solid) material comprising two or more different isotopes by taking advantage of the sedimentation of atoms through an acceleration field by ultra-high speed rotation. A condensation-system (liquid or / and solid) material (5) comprising the two or more isotopes is placed in a sedimentation tank (for example, 2) which is then housed in a supercentrifuge. The supercentrifuge in its rotor is rotation driven by an ultra-high speed rotation power source, and an acceleration field of energy of 100000 G to 1500000 G, i.e., about 100 to 800 m / s in terms of peripheral velocity, is applied to the above condensed (liquid or / and solid) material under such a temperature that is specified by an isotope material to be enriched. In this case, a difference in centrifugal force applied is provided between the isotopes in the condensed (liquid or / and solid) material comprising the at least two isotopes. By virtue of sedimentation by taking advantage of this difference, isotope atoms within the condensed material interact, and, consequently, separation and enrichment of the isotopes can be realized in a higher efficiency than the case where gas is used, by conducting the separation and enrichment of the isotopes within the liquid material, using an effective material, and using a multistaged rotor system.

A system for separating a sample and collecting the separated sample. The system includes an ultracentrifuge having a cylindrical rotor. The system includes a gradient delivery assembly for delivering a gradient solution to the ultracentrifuge rotor, and a sample delivery assembly for delivering the solution containing the sample to the ultracentrifuge rotor. he system includes a fraction collection assembly for collecting discrete volumes of the separated sample. The system includes a processor for controlling operation of the ultracentrifuge, as well as the gradient delivery assembly, the sample delivery assembly, and / or the fraction collection assembly.

The invention relates to cytochrome P450, specifically to a method for measuring the content of a biomarker-cytochrome P450 in earthworm viscera, which comprises the following steps of: (1) soaking a live earthworm in glycerol solution, and immediately dissecting and taking out the viscera thereof; (2) cleaning the viscera with cold saline water, transferring to a homogenization buffer, breaking cells with a glass tissue grinder, centrifuging with a ultra-speed centrifuge at low temperature (3DEG C) and 10,000g for 20min, centrifuging the supernatant at 150,000g for 90min, removing the supernatant, and collecting microsome sediment; and (3) dissolving the microsome sediment in a storage buffer, mixing, and measuring the total amount of cytochrome P450 and the protein content in the microsome suspension. The total amount of cytochrome P450 is quantified with sodium hydrosulfite-reduced CO difference spectra method. The invention has the advantages of simplicity, rapidness, low consumption, and accurate qualification and quantitation.

The invention provides a method for extracting pine nut protein of the Korean pine, relating to a preparation method of pine nut protein of the Korean pine. The invention solves the problems of the prior traditional method that extraction yield of the pine nut protein of the Korean pine is low, activity of the extracted pine nut protein of the Korean pine is not high and the processing technique is complicated. The method has the following technologic steps: selecting pine nuts of the Korean pine, decorticating pine nuts, removing red shell of the pine nuts, grinding, degreasing, soaking and extracting the pine nutsin a highfield; using an overspeed centrifugal machine to separate the extracting solution followed by concentrating; freezing out the extracting solution by a vacuum freeze dryer, thus obtaining the freeze-dried powder of the pine nut protein of the Korean pine. The pine nut protein of the Korean pine prepared in the invention is a natural extractive which has no side effect on human body and has the effects of resisting aging, lengthening longevity, losing weight, lowering blood fat level, resisting cancer, nourishing the five internal organs, resisting fatigue, beautifying and the like. The pine nut protein of the Korean pine prepared in the invention is applicable to food, health products, cosmetics, medicine, biochemistry and other fields.

The invention belongs to the field of coking chemical products in metallurgical coking industry and particularly relates to a technique and a device for preventing deposition and blockage of tar residues of a tar system. The technique is characterized in that fluid dredging devices are arranged at positions liable to generate deposition and blockage in a tar-ammonia water separating tank and a tar storage tank, and the fluid dredging devices are controlled by controllers to periodically work so as to eliminate the blockage phenomenon caused by the deposition of tar residue. The device comprises a first fluid dredging device and a second fluid dredging device, wherein the first fluid dredging device is of a hollow tubular structure and is arranged along the bottom part of a conical groove of the tar-ammonia water separating tank in parallel, and the second fluid dredging device is of a multi-branch tubular structure and is arranged at the bottom part of the tar storage tank in parallel. Compared with the prior art, the technique and the device have the advantages that (1) the tar residue deposition and pipeline blockage phenomena of the equipment are greatly improved; (2) the operation continuity is improved and the maintenance time is greatly decreased; (3) the operation of an ultracentrifuge is stable and the separating effect is greatly improved; and (4) the operating environment is obviously improved and the safety is high.

The invention discloses an extraction method of cervus elaphus linnaeus insulin active factors and relates to a preparation method of the cervus elaphus linnaeus insulin. The invention solves the problems of low extraction rate, low activity and fussy production technique of a traditional method in extracting the cervus elaphus linnaeus insulin active factors. The technical process of the extraction method of the cervus elaphus linnaeus insulin active factors comprises the following steps of: selecting fresh two-branched velvet of a red deer; selecting the one third location of the front end, using a liquid nitrogen and a stirring bead grinding method to crack the cervus elaphus linnaeus, using a strong electric field for extraction, using a low-temperature overspeed centrifuge to separate an extract and remove grease matters, and then using a vacuum scraper type film for condensing and a vacuum freezer for frozen-drying, thus obtaining the frozen-dried powder of the cervus elaphus linnaeus insulin active factors. The cervus elaphus linnaeus insulin prepared by the method is a natural extract, does not have side effects to human bodies, has the effects of reducing blood sugar, resisting aging and fatigue, hairdressing, beautifying, protecting liver and nourishing kidney, and can be applied to the fields such as food, health products, makeup, medicines, biochemical industry, and the like.

A method for determining P450 content in biological tag ¿C earthworm body includes soaking earthworm living body in glycerin then moving it into salt solution for breaking it to be pieces, moving broken matter into homogenizing solution for homogenizing of 30 ¿C 50 second at 6000 ¿C 8000 rpm, then centrifugalizing homogenized matter for 20 ¿C 30 min at 12000 ¿C 15000 g under 3 ¿C 5 Deg C.; removing off supernatant then centrifugalizing for 90 ¿C 120 min at 120000 ¿C 150000g, discarding supernatant and putting particles in ice water, removing off red matter; dissolving particle in buffer solution and taking out 1 ¿C 2 mI for detecting protein content as the rest for being used to determine P450 content .

Provided herein is a method for treating a pulmonary disease using mesenchymal stem cell-derived artificial nanosomes exhibiting a significantly excellent capability to regenerate alveoli compared to mesenchymal stem cells themselves and natural exosomes derived from human adipose-derived mesenchymal stem cells, wherein the mesenchymal stem cell-derived artificial nanosomes of the present disclosure may be collected through a simple production method using an extruder and an ultracentrifuge, instead of treating mesenchymal stem cells with a separate chemical substance and the potential side effects caused by stem cell therapeutic agents is low.

The invention discloses an elastic damping support for a motor rotor of an ultracentrifuge, which comprises the structures of O-shaped rubber rings, a high-speed bearing, a supporting seat, the motor rotor, a motor stator and a motor shell, wherein the outer sides of the high-speed bearing at two ends of the rotor are provided with O-shaped rubber ring bases, and the O-shaped rubber rings are arranged between the supporting seat and the O-shaped rubber ring bases. The elastic damping support overcomes the defects that a domestic product has low precision, easy heat generation and short service life, and simultaneously solves the manufacturing difficulty of using metal rings in foreign countries; besides, the operation of the elastic damping support is better than the motor with the foreign metal rings, and the elastic damping support replaces the import and widens the international market.

A method for preparing compound with hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors relates to a method for preparing cornu cervi pantotrichum insulin and a method for preparing a compound with hypoglycemic function. The invention solves the problems of low extraction rate in extracting the cornu cervi pantotrichum insulin active factors, low activity and complicated production process in the existing traditional methods. The technical steps of the method for preparing the compound with hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors are as follows: selecting fresh two branched antler of red deer as well as the one third position of the tip; using a liquid nitrogen and agitated bead grinding method to crush the cornu cervi pantotrichum; separating extracting solution by using a low temperature supercentrifuge by a high field for extraction, and removing lipids; then using a vacuum scraper membrane for concentration and a vacuum freeze dryer for freeze-drying; and subsequently compounding the obtained freeze-dried cornu cervi pantotrichum insulin active factor powder with seabuckthorn flavone, auricularian and balsampear fruit extract to prepare the compound with the hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors. The compound with the hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors prepared has no side effect on the human body but the functions of reducing blood sugar and blood fat, resisting fatigue, beautifying and keeping youthfulness, and protecting the liver and nourishing the kidney, and can be used in the fields of food, health care products, cosmetics, medicines, etc.

The invention discloses a preparing and collecting method for starch particles. The preparing and collecting method comprises the steps that starch is subjected to superfine grinding and then prepared into a starch solution with a certain concentration; the starch solution is subjected to homogenization with a high-pressure homogenizing machine and treated with a high-speed fluidizing device and then passes through a super centrifuge, centrifugal separation is carried out at different rotating speeds, and starch particles of different sizes are obtained; finally, the starch solution containing starch particles of different sizes is led into an electrophoresis tank, a cationic surfactant is added, polar plates in different shapes or with specific patterns are arranged, and starch particles assembled in a patterned mode in different sizes are obtained on a collector. According to the preparing method, the starch particles obtained after superfine grinding in combination with high-speed fluidizing are obvious in damage of crystalline structures, active groups are fully exposed, the action between the starch particles and the cationic surfactant is easier, and the collected starch particles with different patterns and different sizes are formed at a time and uniform and can be widely applied in the fields of preparing functional starch films, patterned paper and the like.

The invention provides a high-tensile strength platycodon edible film and a preparation method thereof, and relates to a platycodon edible film product and a preparation method thereof. The preparation method of the high-tensile strength platycodon edible film comprises the following steps of: preparing moulding solution with 2.0%-3.0% (weight) of platycodon polysaccharide extractive, 2.5%-3.0% of sodium alga acid, 1.5%-2.0% of glycerol and 80-95% of dissolvent; stirring; degassing; paving a film; and drying to obtain the high-tensile strength platycodon edible film with 0.05-0.2mm. Specifically, the preparation technology of the high-tensile strength platycodon edible film comprises the following steps of: selecting the dried root of the platycodon; degreasing the smashed powder; extracting the polysaccharide extractive from the platycodon through a microwave auxiliary extraction method; separating the extracting liquid with an ultracentrifuge; concentrating with a vacuum rotary evaporator; drying in a freeze way with a vacuum freeze dryer to obtain freeze-drying powder of the platycodon polysaccharide extractive; matching the freeze-drying powder with proper amount of intensifier and plasticizer to prepare film liquid; stirring; degassing; paving a film; and drying to obtain the platycodon edible film with better tensile strength.

A method for preparing beauty mask containing active compound of pilose antler and traditional Chinese medicine composition relates to a method for extracting active compound of pilose antler and a method for preparing beauty mask. The invention settles the problems of low extraction rate, low activity and tedious production process when a prior traditional method is used for extracting active compound of pilose antler. The method for preparing beauty mask containing active compound of pilose antler comprises the following steps: selecting fresh two-branched antler of cervus elaphus, selecting a one-third part at the front end, crushing the pilose antler of cervus elaphus with liquid nitrogen through a mixing-bead milling method, extracting through highfield, separating the raffinate with a low-temperature ultracentrifuge, eliminating the lipid matter, condensing with a vacuum blade type film, mixing the concentrated solution of active compound of pilose antler, Auricularia Auricular polysaccharide, ginseing extract, angelia extract and Bletilla striata extract, milling with a colloid mill and homogenizing for preparing the beauty mask containing the active compound of pilose antler. The beauty mask containing active compound of pilose antler and traditional Chinese medicine composition, which is prepared according to the invention, has no side effect for human body, has the functions of beautifying and face nursing, and can be applied for the fields of beautifying, cosmetic manufacturing, etc.

The invention relates to an efficient preparation method for for acquiring phellinus igniarius polyphenols from submerged fermentation mycelia of phellinus igniarius. The method comprises the following steps: inoculating a phellinus igniarius strain into a phellinus igniarius mycelium culture medium, carrying out submerged fermentation culture on the inoculated phellinus igniarius mycelium culture medium, freeze-drying the obtained phellinus igniarius mycelia, conducting pulverizing, and carrying out screening with a 40-mesh sieve to obtain phellinus igniarius mycelium powder; mixing the S phellinus igniarius mycelium powder with a deep eutectic solvent having a water content of 30%-50% according to a mass ratio of 1: 30 to 1: 50; extracting an S mixed solution in an ultrasonic extractor for 30-50 minutes, conducting centrifuging in an ultracentrifuge, and taking a supernatant; separating and purifying the supernatant through D101 macroporous resin to obtain a refined phellinus igniarius polyphenol mixed solution, and removing ethanol from the mixed solution through rotary evaporation to obtain refined phellinus igniarius polyphenols. According to the method, phellinus igniarius polyphenol can be more effectively extracted, the phellinus igniarius mycelia can be fully utilized, resources are not wasted, and the method is environmentally friendly.

The invention discloses a dechlorination method for tar. The method comprises the following steps: heating tar and water to 70-95 DEG C, and fully mixing the two, wherein the adding amount of the water accounts for 5-25% of the mixture of the tar and the water in percentage by mass; at a temperature of 80-95 DEG C, after the tar is fully standing, separating the tar so as to obtain an oil phase and an upper high-chlorine water phase, and then carrying out further centrifugal separation on the obtained oil phase by an ultracentrifuge at a temperature of 50-90 DEG C so as to obtain low-chloride tar and a high-chlorine water phase. According to the invention, through a water washing process, most of chlorine in tar is removed, so that an effect that no alkali is added in tar processing, no corrosion is increased, the metal content of asphalt products such as sodium ions and the like obtained by distillation is low, and useful substances such as phenol and ammonia and the like in the upper high-chlorine water phase obtained after separation also can be recycled is achieved, therefore, the method is economic and environmental-friendly.

A fluid handling system is provided that includes a controller, a plurality of valves, a pump, and an interface in communication with the controller. The valves can be operatively connected with a conduit to along a flow path. Each of the valves is controlled by the controller for selective movement between a first position and a second position. The first position closes the flow path, while the second position opens the flow path. The pump can be operatively connected with the conduit and the is controlled by the controller for selective pumping of fluid through the flow path. The interface allows selective definition of the flow path through the valves.