Method for extracting platycodon root polysaccharide

A technology of platycodon grandiflorum polysaccharide and extraction method, which is applied to the preparation field of platycodon grandiflorum polysaccharide, can solve the problems of low activity and low extraction rate of platycodon grandiflorum polysaccharide, and achieves the effects of high extraction rate, improved economic value and simple process

Inactive Publication Date: 2010-09-15
王振宇
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  • Summary
  • Abstract
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Problems solved by technology

[0003] In order to solve the problems of low extraction rate and low activity of platycodon polysaccharides, the present invention provides a method for...

Method used

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specific Embodiment approach 1

[0005] Specific embodiment one: in this specific embodiment, a kind of extraction method of platycodon grandiflorum polysaccharide is characterized in that the step of the method for platycodon grandiflorum extraction is as follows: one, choose dry platycodon grandiflorum rhizome, pulverize 60 mesh sieves, with certain feed liquid The ratio of 1g: 20mL ~ 1g: 60mL is added to platycodon root powder and aqueous solution respectively, soaked for 1 hour, extracted with a certain microwave power, and then bathed in water at 40-70°C to obtain an extract rich in platycodon grandiflorum polysaccharide. Carry out solid-liquid separation with a decanter centrifuge at a speed of 1500-2000r / min, and separate the extract and residue; 2. Use the sevag method (chloroform: n-butanol = 4: 1) to repeatedly deproteinize, and dialysis bag for dialysis , 45 ~ 55 ℃ vacuum rotation instrument to concentrate the extract; 3. After measuring the absorbance value of the solution with the phenol-sulfuric ...

specific Embodiment approach 2

[0006] Embodiment 2: This embodiment is different from Embodiment 1 in that: In Step 1, the microwave power is 300-500 W for 1-4 minutes. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0007] Embodiment 3: This embodiment is different from Embodiment 1 in that: the water bath time in step 1 is 40-60 minutes. The centrifugation time is 10-20 minutes. Others are the same as in the first embodiment.

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Abstract

The invention discloses a method for extracting platycodon root polysaccharide, which relates to a method for preparing the platycodon root polysaccharide and solves the problems of low yield, long consumed time, low polysaccharide activity and fussy process in the conventional platycodon root polysaccharide extraction process. The method for extracting the platycodon root polysaccharide comprises the following steps of: selecting dry platycodon root, degreasing the crushed powder, then extracting polysaccharide from the platycodon root by a microwave-aid water extracting method to obtain a polysaccharide extract, removing protein and then separating the extract by using an ultra-speed centrifuge, concentrating the extract by using a film under vacuum, and freeze-drying the extract by using a vacuum freeze dryer after dialysis concentration to obtain platycodon root polysaccharide freeze-drying powder. The platycodon root polysaccharide extracted by the method has the effects of enhancing immunologic function, resisting tumor, reducing blood fat and blood pressure, resisting cruor and thrombus, losing weight, caring skin, promoting the healing of cornea wound, improving dry eyes and the like. The platycodon root polysaccharide can be applied in the fields of food, health-care products, cosmetics, medicaments, biochemical engineering and the like.

Description

technical field [0001] The invention relates to a preparation method of bellflower polysaccharide. Background technique [0002] The traditional extraction method of Platycodon grandiflorum polysaccharides is mainly based on water-bath extraction, which is time-consuming, labor-intensive, and consumes a lot of ethanol, and the extraction rate of polysaccharides is low and the cost is high. At present, ultrasonic, supercritical extraction technology, microwave-assisted method and enzymatic method are also used to extract polysaccharides. Ultrasonic extraction can greatly shorten the extraction time, improve work efficiency and save solvents, but because the cavitation effect of ultrasonic waves has a destructive effect on macromolecular sugar chains, the molecular weight of the extracted polysaccharides is reduced and the original polysaccharide structure is destroyed; However, supercritical extraction technology cannot be widely used in a large amount of extraction process,...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王振宇焦岩王会友
Owner 王振宇
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