30 results about "Perfusion rate" patented technology

A method and apparatus for noninvasive glucose measurement measures glucose indirectly from the natural response of tissue to variations in analyte concentration. The indirect measurement method utilizes factors affected by or correlated with the concentration of glucose, such as refractive index, electrolyte distribution or tissue scattering. Measurement reliability is greatly improved by stabilizing optical properties of the tissue at the measurement site, thus blood perfusion rates at the sample site are regulated. Perfusion is monitored and stabilized by spectroscopically measuring a control parameter, such as skin temperature, that directly affects perfusion. The control parameter is maintained in a range about a set point, thus stabilizing perfusion. Skin temperature is controlled using a variety of means, including the use of active heating and cooling elements, passive devices, such as thermal wraps, and through the use of a heated coupling medium having favorable heat transfer properties.

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

The present invention relates to an apparatus for measuring the perfusion rate of legs. The measurement apparatus of the present invention includes a light leakage prevention unit including a light emitting device for radiating light having a certain wavelength onto a living body injected with Indocyanine Green (ICG), and a light leakage prevention housing formed to prevent transmission of external light. A data acquisition unit includes a band pass filter for passing therethrough only light, having a near infrared wavelength, in a fluorescence image emitted by the ICG and the light emitting device, and a Charge Coupled Device (CCD) camera for photographing a near infrared wavelength region. A data processing unit calculates a perfusion rate of the living body on a basis of data about the near infrared wavelength region through a data cable connected to the data acquisition unit. A data output unit outputs the perfusion rate calculated by the data processing unit.

Techniques are provided for use by a pacemaker or other implantable medical device for detecting and tracking trends in cardiopulmonary fluid transfer rates—such as heart-to-lung fluid perfusion rates and lung-to-lymphatic system fluid excretion rates—and for detecting heart failure, dyspnea or other cardiopulmonary conditions. In one example, the device periodically measures transthoracic admittance values. A first exponential time-constant (k1) is determined using curve-fitting from admittance values obtained while the patient is in a sleep posture. Time-constant k1 is representative of the fluid perfusion rate. A second exponential time-constant (k2) is determined based on admittance values obtained while the patient is standing / walking / sitting. The second exponential time-constant (k2) is representative of the fluid excretion rate from the lungs. The device then detects trends, if any, in the time-constants (or in “DC” baseline values) to detect or predict medical conditions such as an imminent heart failure exacerbation.

The invention discloses a temperature control gas phase working medium perfusion method for a micron heat pipe, which is applied to heat dissipation of high heating flux devices. The method is characterized in that the temperature of the micron heat pipe and the temperature of a working medium container are respectively controlled; the micron heat pipe and the working medium container are respectively vacuumized; then the working medium container is connected with a working medium source, so that working medium steam can get into the working medium container; the micron heat pipe and the working medium container are communicated, so that perfusion of the gas phase working medium can be realized by utilizing the temperature difference between the micron heat pipe and the working medium container; the perfusion amount of the working medium can be controlled as per time, and after a perfusion port of the micron heat pipe is sealed, working medium perfusion for the micron heat pipe is accomplished. The working medium exists in a perfusion system in gas phase, so the perfusion rate, the repeatability and the controllability are good; the temperature of the micron heat pipe and the temperature of the working medium are respectively controlled, so that the perfusion efficiency of the working medium is improved; the method can improve the performance of the micron heat pipe.

The invention discloses a technology for producing a recombinant antibody protein through the high density perfusion culture of hybridoma cells. The technology which aims at the metabolic characteristic of the hybridoma cells screens a specific culture component for the perfusion culture, and allows metabolic substrates to be fully supplied in the culture process and the concentration of metabolic byproducts to be simultaneously and effectively reduced, so objects of high efficiency and high yield is reached. In the process control aspect, a specific perfusion rate CSPR perfusion culture calculating model is established to carry out real time calculation on culture parameters, and concentrated medium subsection fed batch is adopted to control the perfusion rate, so important substrates ofglucose and glutamine are supplied and the influence of the byproducts to culture is minimized. The technology which is suitable for the preparation of the production antibody protein through the large scale culture of the hybridoma cells allows a large amount of protein antibodies to be provided in a short time, has the advantages of small scale, low investment, realization of the high efficiency output in a short time, low batch difference, high repeatability, and realization of the production of antibody medicines.

A method and apparatus for noninvasive glucose measurement measures glucose indirectly from the natural response of tissue to variations in analyte concentration. The indirect measurement method utilizes factors affected by or correlated with the concentration of glucose, such as refractive index, electrolyte distribution or tissue scattering. Measurement reliability is greatly improved by stabilizing optical properties of the tissue at the measurement site, thus blood perfusion rates at the sample site are regulated. Perfusion is monitored and stabilized by spectroscopically measuring a control parameter, such as skin temperature, that directly affects perfusion. The control parameter is maintained in a range about a set point, thus stabilizing perfusion. Skin temperature is controlled using a variety of means, including the use of active heating and cooling elements, passive devices, such as thermal wraps, and through the use of a heated coupling medium having favorable heat transfer properties.

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

Described herein is a rapid continuous biomanufacturing platform process that combines a perfusion mammalian cell culture with a synchronized purification of the antibodies produced by the cell culture without the use of intermediate media holding tanks or other large retention devices. This method described herein includes continuous cell culture of mammalian cells expressing the biological of interest and comprising a cell retention device wherein the perfusion of fresh media into the reactor and hence the harvest rate of antibody containing spent media from the reactor has a rate of 1 vessel volume per day (vvd) or less obtained by glucose control with a cell density of between 40 million cell / ml and 60 million cell / nil. Immediate recovery and purification of the antibody is obtained by synchronizing the rate of perfusion with the antibody capture and elution step cycle in a column that contains affinity resin that is between 0.01 and 0.001 times the volume of the bioreactor. The perfusion rate and capture rate were perfectly synchronized by extensive method and media development resulting in equal rates and eliminating the need for holding tanks and cell bleeding. The result is an order of magnitude faster antibody production as compared to conventional fed-batch mode.

The combined multiple-antenna and heat field method for microwave intervention treatment of tumor includes the following steps. In the first step, the real-time 3D solidification heat field distribution of single antenna under different radiation power is obtained via measurement and calculation. In the second step, 3D solidification heat field distribution data base is found out for two or more antennas via permutation and combination to establish computer model and analysis with the limited element software based on the conditions of antennas, power, time of different power and blood stream perfusion rate of cancerized tissue. In the third step, the temperature boundary condition for the treatment after being 3D reconstructed is added into the heat field distribution data base in different conditions to form in the computer real-time heat field distribution data and corresponding 3D solidification heat field illustration under the different condition parameters.

A body temperature detector is particularly suited to axillary temperature measurements of adults. The radiation sensor views a target surface area of the body and electronics compute an internal temperature of the body as a function of ambient temperature and sensed surface temperature. The function includes a weighted difference of surface temperature and ambient temperature, the weighting being varied with target temperature to account for varying perfusion rate. Preferably, the coefficient varies from a normal of about 0.13 through a range to include 0.09. The ambient temperature used in the function is assumed at about 80° F. but modified with detector temperature weighted by 20%.

The invention provides a method for preparing rabies vaccines for human use, which is used for solving the problems of low virulence and low yield of viruses harvested after virus vaccination in the prior art. The method for preparing rabies vaccines for human use comprises the steps of 1) reviving and inheriting production cells, and then putting the inherited production cells into a microcarrier bioreactor for multiplication culture; 2) adding a treatment solution into the microcarrier bioreactor, adjusting the perfusion rate of the microcarrier bioreactor to be zero, and cleaning the surfaces of the production cells; 3) vaccinating rabies viruses to the cleaned production cells to fix strains; 4) harvesting a virus solution, and performing ultra-filtration concentration and inactivation; 5) separating and purifying. The method disclosed by the invention has the advantages that before the viruses are vaccinated, the cells are treated by using the treatment solution, so that the quantity of the viruses entering the cells is increased; after the viruses are vaccinated, the yield of the viruses is greatly improved, and the times of ultra-filtration concentration is reduced.

A microflow perfusion method and its apparatus feature that a hydroscopic expending body in the casing is used to apply a force to a liquid storage bag for pressing it, and the expanding rate of said hydroscopic expending body and in turn the perfusion rate of liquid storage bag can be controlled by the water-sucking tube which has a selective diameter of its internal channel. After said diameter of internal channel, the flow of perfusion is constant.

Techniques are provided for use by a pacemaker or other implantable medical device for detecting and tracking trends in cardiopulmonary fluid transfer rates—such as heart-to-lung fluid perfusion rates and lung-to-lymphatic system fluid excretion rates—and for detecting heart failure, dyspnea or other cardiopulmonary conditions. In one example, the device periodically measures transthoracic admittance values. A first exponential time-constant (k1) is determined using curve-fitting from admittance values obtained while the patient is in a sleep posture. Time-constant k1 is representative of the fluid perfusion rate. A second exponential time-constant (k2) is determined based on admittance values obtained while the patient is standing / walking / sitting. The second exponential time-constant (k2) is representative of the fluid excretion rate from the lungs. The device then detects trends, if any, in the time-constants (or in “DC” baseline values) to detect or predict medical conditions such as an imminent heart failure exacerbation.

A body temperature detector is particularly suited to axillary temperature measurements of adults. The radiation sensor views a target surface area of the body and electronics compute an internal temperature of the body as a function of ambient temperature and sensed surface temperature. The function includes a weighted difference of surface temperature and ambient temperature, the weighting being varied with target temperature to account for varying perfusion rate. Preferably, the coefficient varies from a normal of about 0.13 through a range to include 0.09. The ambient temperature used in the function is assumed at about 80° F. but modified with detector temperature weighted by 20%.

The invention discloses an ophthalmic collagen cross-linking integrated device which comprises a working device, a control device, a perfusion device, a light source device and a power supply device,wherein the power supply device is electrically connected with the working device, the infusion device, the control device and the light source device respectively, the perfusion device is connected to the working device through a conduit, the light source device and the working device are connected by light, the control device is connected with the working device, the perfusion device and the light source device by a wireless or wired manner, the devices can be fixedly connected or actively connected, and the working device is used for alignment of treatment parts, the control device is usedfor displaying and controlling the working state, the perfusion device is used for controlling the perfusion rate of a riboflavin solution, and the light source device is used for generating therapeutic light. The device is small in size, quick and convenient to carry, simple and flexible to operate, integrates ultraviolet light irradiation and riboflavin perfusion in one, not only improves the accuracy of the treatment process, but also liberates the hands of an operator, makes a treatment system more efficient and flexible.

The invention discloses a self-adaptive radiotherapy method based on image processing, and particularly relates to the technical field of image processing. The method mainly comprises the steps of obtaining a tissue density image of a target tissue in an initial state, and screening a region in a preset density range according to the tissue density image as a target region, setting an initial adjustment amount according to the density of the target region, according to the initial adjustment amount, adjusting the tissue density at the target region back, and starting to heat the tissue at the target region, in a heating state, calculating a current density influence parameter according to the real-time temperature, and calling a corresponding scaling coefficient from the contrast relation table according to the density influence parameter, and scaling the initial adjustment amount according to the scaling coefficient, and callback the tissue density at the target region according to the scaled adjustment amount. The scaling coefficient of the regulating variable is adjusted in real time according to the temperature field, the heat loss degree and the perfusion rate, so that the regulating variable can dynamically change according to the change of the activation energy of the biological tissue.

The present disclosure relates to method for predicting location and depth of abnormal tissue in breast tissue by prediction system. The prediction system predicts location based on 2D thermal image generated based on temperature values and intermediate temperature values. The intermediate temperature values are estimated using triangular and rectangular patterns formed on pre-defined model of breast, thermal conductivity of breast tissue, 2D coordinates on one of triangular and rectangular patterns and temperature values at steady state of breast tissue. The depth is predicted based on 3D thermal image of breast tissue generated using temperature values and intermediate temperature values and error parameter. The intermediate temperature values are estimated based on one of tetrahedral and hexahedral patterns formed on predefined model of breast, density value, thermal conductivity of tissue, blood perfusion rate, specific heat capacity of blood, 3D coordinates, arterial temperature and metabolic heat generation value in normal and abnormal tissue.

The invention discloses an adaptive radiotherapy method based on image processing, specifically relates to the technical field of image processing, and mainly includes the steps of: acquiring a tissue density image in an initial state of a target tissue, and screening out tissue density images within a preset density range according to the tissue density image The area is used as the target area; the initial adjustment amount is set according to the density of the target area; the tissue density at the target area is called back according to the initial adjustment amount, and the tissue at the target area is started to be heated; in the heating state, the current density influencing parameters are calculated according to the real-time temperature , and the corresponding scaling factor is retrieved from the comparison table according to the density-affecting parameter; the initial adjustment amount is scaled according to the scaling factor, and the tissue density at the target area is recalled according to the adjusted adjustment amount after scaling. The invention adjusts the scaling coefficient of the adjustment amount in real time according to the temperature field, the degree of heat loss and the perfusion rate, so that the adjustment amount can be dynamically changed according to the change of the activation energy of the biological tissue.

The invention provides a method for nondestructively measuring thermal parameters of living biological tissue, which is used for determining the thermal parameters of cylindrical living living tissue through three-point surface temperature measurement. The method includes: establishing a two-dimensional Pennes heat transfer model of a cylindrical living body tissue under cylindrical coordinates; using a row-by-row method to solve the temperature change in the cylindrical living body tissue under the interference of a step heat flow or a square wave heat flow; , analyze the correlation between the sensitivity coefficients of thermal parameters and the influence of thermal parameters on temperature changes, and optimize the selection of three measurement points; develop a measurement system for the surface temperature of biological living tissue, and collect temperature transient response data in real time; application improvement Using the Gaussian parameter estimation method, thermal parameters such as thermal conductivity, thermal diffusivity, blood perfusion rate, and volumetric heat capacity of cylindrical biological living tissue are obtained at the same time. The advantage is that it realizes convenient and simple non-destructive measurement.

A framework for the accurate and noninvasive determination of perfusion in a mammal is provided, including a novel scaling law of a form-function relationship between the number of capillaries in a vascular network as compared to the perfusion of such network. Methods are disclosed that apply such scaling laws in connection with the steps of determining a capillary density of a targeted tissue comprising at least a portion of a capillary network, and calculating perfusion of the targeted tissue based on the determined capillary density of the targeted tissue. Additional methods for determining a therapeutic drug dosage for a biological subject are also provided based on the scaling-laws hereof, as well as methods of identifying a deviation in perfusion rates in a mammal noninvasively.

The invention discloses an Expi293F cell culture method, which comprises the following steps: inoculating Expi293F cells into a bioreactor for culture, and carrying out perfusion dynamic culture for 32-48 hours: uniformly dividing a culture solution to be perfused into a plurality of strands, and perfusing into the bioreactor along the inner wall of the bioreactor in a wall-attached manner, with the stirring speed in the bioreactor being 80-90 rpm and the perfusion rate of the perfused solution being 0.5-2 working volumes / d, during dynamic perfusion culture, the culture conditions in the reactor are kept as follows: the temperature is 36.5-37 DEG C, the DO is 40-60%, and the pH is 7.2 + / -0.2; a serum-free culture solution used in the culture process is prepared from 95wt% of 24.75 g / L Dynami culture medium, 4wt% of 200mM glutamine and 1wt% of an anti-caking agent. According to the invention, the perfusion mode is improved, and proper perfusion rate and stirring strength reduction are matched, so that the injection of the culture medium in the perfusion process also plays roles in expanding mass transfer, reducing the influence of cell agglomeration on cell metabolism and reducing physical damage to cells, high-density cells and high survival rate are obtained, and remarkable effects are achieved.

The invention discloses a technology for producing a recombinant antibody protein through the high density perfusion culture of hybridoma cells. The technology which aims at the metabolic characteristic of the hybridoma cells screens a specific culture component for the perfusion culture, and allows metabolic substrates to be fully supplied in the culture process and the concentration of metabolic byproducts to be simultaneously and effectively reduced, so objects of high efficiency and high yield is reached. In the process control aspect, a specific perfusion rate CSPR perfusion culture calculating model is established to carry out real time calculation on culture parameters, and concentrated medium subsection fed batch is adopted to control the perfusion rate, so important substrates ofglucose and glutamine are supplied and the influence of the byproducts to culture is minimized. The technology which is suitable for the preparation of the production antibody protein through the large scale culture of the hybridoma cells allows a large amount of protein antibodies to be provided in a short time, has the advantages of small scale, low investment, realization of the high efficiency output in a short time, low batch difference, high repeatability, and realization of the production of antibody medicines.

This application relates to the field of biomedical engineering, and discloses a method and system for evaluating the individualized blood perfusion rate of biological tissues. Based on the influence of peripheral blood flow on the thawing process of the target biological tissue after freezing, the calculation of local tissue is established by the principle of boundary convection heat transfer. An algorithm for personalized blood perfusion rate, the method includes: obtaining the surface area of ​​the ice ball formed by freezing the target biological tissue; obtaining the temperature gradient of the ice ball surface and its change over time according to the temperature of the ice ball surface The gradient and its variation with time and the surface area of ​​the ice ball determine the personalized blood perfusion rate around the target biological tissue; output the personalized blood perfusion rate around the target biological tissue.

The present invention relates to cell culture in bioreactors, such as flexible cellbag bioreactors. More closely the invention relates to a method and system for determining the cell density in a bioreactor culture and for controlling the perfusion rate of a suspension culture of cells in a bioreactor, comprising measuring the oxygen uptake of primary mononuclear cells in a non-static bioreactor.

The invention relates to the field of biomedical engineering, and discloses a biological tissue personalized blood perfusion rate evaluation method and system. The biological tissue personalized blood perfusion rate evaluation method comprises the steps that an algorithm for calculating the local tissue personalized blood perfusion rate is calculated through a boundary convective heat transfer principle based on the influence of peripheral blood flow on the thawing process of a target biological tissue after freezing; the surface area of an ice ball formed by freezing a target biological tissue is obtained; the temperature gradient of the surface of the ice ball and the change of the ice ball along with time are obtained, and the personalized blood perfusion rate around the target biological tissue is determined according to the temperature gradient of the surface of the ice ball, the change of the ice ball along with time and the surface area of the ice ball; and the personalized blood perfusion rate around the target biological tissue is output.

The invention discloses a method for separating and preparing primary hepatocytes, which comprises the following steps: a. Extracorporeal perfusion of the liver: take the isolated animal liver, and first perfuse it with perfusate I at a rate of 300-1000mL / min, The perfusion time is 10-12 minutes; then perfusate with perfusate II, the perfusion rate is 300-1000mL / min, and the perfusion time is 2-3 minutes; finally perfusate with perfusate III, the perfusion rate is 400-1200mL / min, the perfusion time 25‑40 minutes; b. Separation of liver cells: wash the liver treated in step a, collect the liver cell suspension, filter, centrifuge, and collect the precipitate. The method for separating and preparing primary hepatocytes of the present invention can isolate and obtain hepatocytes with high quantity, viability and purity, and has a good application prospect.