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Method for preparing rabies vaccines for human use

A rabies vaccine and rabies virus technology, applied in the field of vaccines, can solve the problems of small yield and low virulence of harvested viruses, and achieve the effects of stabilizing the filtration speed, increasing the amount of virus production, and reducing accumulation

Inactive Publication Date: 2014-09-10
SHANDONG YIDU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention proposes a preparation method of human rabies vaccine, which solves the problems of low virus toxicity and small yield in the prior art after virus inoculation

Method used

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  • Method for preparing rabies vaccines for human use
  • Method for preparing rabies vaccines for human use
  • Method for preparing rabies vaccines for human use

Examples

Experimental program
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Effect test

preparation example Construction

[0034] Such as figure 1 Shown, a kind of preparation method of human rabies vaccine comprises the following steps:

[0035] 1) recovery and subculture of production cells, and then placing the subcultured production cells in a microcarrier bioreactor for expansion and culture;

[0036] 2) Add treatment solution to the microcarrier bioreactor, adjust the perfusion velocity of the microcarrier bioreactor to zero, and clean the surface of the production cells;

[0037] 3) Inoculating the fixed strain of rabies virus on the washed production cells;

[0038] 4) Harvest the virus liquid, carry out ultrafiltration concentration, inactivation;

[0039] 5) separation and purification.

[0040] As a preferred technical scheme, the treatment liquid in the step 2) is a sterile PBS solution containing sodium butyrate and protamine sulfate solution, the concentration of the sodium butyrate is 0.1~1mmol / l, and the fish sulfate The concentration of the protamine solution is 80-120 μg / ml, ...

specific Embodiment

[0049] Experiment 1. Virus culture and harvest

[0050] Experimental Materials:

[0051] Cells: Vero cells, PV strains

[0052] Culture medium: Improved MEM liquid medium, that is, adding 1000-3000 mg / L glucose to the basic MEM culture medium.

[0053] Main equipment: bioreactor (BIO-FLO110, NBS)

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Abstract

The invention provides a method for preparing rabies vaccines for human use, which is used for solving the problems of low virulence and low yield of viruses harvested after virus vaccination in the prior art. The method for preparing rabies vaccines for human use comprises the steps of 1) reviving and inheriting production cells, and then putting the inherited production cells into a microcarrier bioreactor for multiplication culture; 2) adding a treatment solution into the microcarrier bioreactor, adjusting the perfusion rate of the microcarrier bioreactor to be zero, and cleaning the surfaces of the production cells; 3) vaccinating rabies viruses to the cleaned production cells to fix strains; 4) harvesting a virus solution, and performing ultra-filtration concentration and inactivation; 5) separating and purifying. The method disclosed by the invention has the advantages that before the viruses are vaccinated, the cells are treated by using the treatment solution, so that the quantity of the viruses entering the cells is increased; after the viruses are vaccinated, the yield of the viruses is greatly improved, and the times of ultra-filtration concentration is reduced.

Description

technical field [0001] The invention relates to the field of vaccines, in particular to a preparation method of a human rabies vaccine. Background technique [0002] Rabies is a mammalian disease affecting mainly canids (dogs, wolves, foxes, mountain dogs, and jackals), procyonidae (raccoons), civets (mongooses), skunks ( skunk) and chiroptera (bats) as hosts or carriers. All mammals are susceptible to this disease. [0003] Almost all human rabies infections are secondary to animal bites or scratches. Infection of the cornea, tissues, and organs by virus inhalation, vaccination with incompletely inactivated vaccines, or transplantation has also occurred. Dogs are the main host of human rabies in most of the world, although cats are not hosts of rabies virus, but they are also important carriers of the virus. Rabies is mainly manifested as an acute invasive viral encephalitis, transmitted by exposure to saliva of infectious virus or other routes, and the disease can be t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/205C12N7/00C12N7/02A61P31/14
Inventor 王雪云梁海岩高红云刘帅赵小娥樊可董文娟李良褚秀远
Owner SHANDONG YIDU BIOTECH
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