The invention provides a method for preparing rabies vaccines for human use, which is used for solving the problems of low virulence and low yield of viruses harvested after virus vaccination in the prior art. The method for preparing rabies vaccines for human use comprises the steps of 1) reviving and inheriting production cells, and then putting the inherited production cells into a microcarrier bioreactor for multiplication culture; 2) adding a treatment solution into the microcarrier bioreactor, adjusting the perfusion rate of the microcarrier bioreactor to be zero, and cleaning the surfaces of the production cells; 3) vaccinating rabies viruses to the cleaned production cells to fix strains; 4) harvesting a virus solution, and performing ultra-filtration concentration and inactivation; 5) separating and purifying. The method disclosed by the invention has the advantages that before the viruses are vaccinated, the cells are treated by using the treatment solution, so that the quantity of the viruses entering the cells is increased; after the viruses are vaccinated, the yield of the viruses is greatly improved, and the times of ultra-filtration concentration is reduced.