138 results about "Microcarrier" patented technology

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The embodiment of the invention discloses a method for constructing a cartilage micro-tissue by compounding an extracellular matrix with a vascular matrix component and application. The method comprises the following steps: preparing a porcine decellularized cartilage extracellular matrix; generating a cartilage extracellular matrix suspension; carrying out screening treatment on the cartilage extracellular matrix suspension; preparing a porcine acellular cartilage extracellular matrix microcarrier; preparing a target suspension of a common system from the porcine acellular cartilage extracellular matrix microcarrier and vascular matrix component cells, and transferring the target suspension to a bionic floating microenvironment of a shaker bioreactor for dynamic culture; carrying out culture treatment on the porcine acellular cartilage extracellular matrix microcarrier; and taking out the cell-loaded porcine decellularized cartilage extracellular matrix microcarrier from the cultured and treated porcine decellularized cartilage extracellular matrix microcarrier as the constructed cartilage micro-tissue. According to the embodiment, the condition of secondary injury to a user can be improved and reduced, the repairing effect is improved, and the adaptability of the constructed cartilage micro-tissue is improved.

The invention relates to image differentiated multiplex assays. Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarrier is encoded using an analog code for identification and includes a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits related to multiplex assays using the microcarriers described herein.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

Devices for filtering liquids are provided herein. More particularly, the devices may be devices for separating biological cells from microcarriers the cells have been grown with, as well as other debris and materials, that are found in the cell culturing liquid. The devices may also be foldable or collapsible.

The application provides a method for preparing FFPE quality control products by using cell lines, a reference product and a kit, and belongs to the fields of medicine, clinical laboratory science and biotechnology, and comprises the following steps: culturing cell lines by using microcarriers; mixing different cell lines based on requirements; centrifuging the mixed cell microcarrier suspension in a sealed malt pipe at one end; removing supernatant after the treatment; packaging the two ends of the malt pipe by using a semi-permeable membrane; removing the semi-permeable membrane after fixing, dehydrating and transparentizing the malt pipe by using a formaldehyde solution; separating the cell microcarriers after the wax immersion treatment from the malt pipe; and embedding the cell microcarriers into a paraffin block by using wax. The quality control product can be used in subsequent quality control processes, the mutation frequency of the quality control product can be kept in good uniformity, and batch production and large-scale production of the quality control product can be realized.

The present invention relates to a single-phase, spherical edible microcarrier for the three-dimensional culture of stem cells intended for the preparation of cultured meat. The microcarrier comprises a homogeneous mixture of edible ionic polysaccharide (0.375-1.5%), edible protein (1-2.5%), edible polymer (0.025-0.1%) and water (95.9-98.6%). The invention also relates to a method for preparing these microcarriers by mixing three solutions: ionic polysaccharide in saline solution, edible protein in saline solution and edible polymer in water. The mixture is subjected to a process involving microspraying or electrospraying with calcium chloride in order to form spherical microcarriers of 50-300 micrometres. The design of the single-phase, spherical edible microcarrier simplifies the production of cultured meat by dispensing with the need for core-shell structures, thereby improving cell anchorage efficiency and facilitating cell recovery.

A system, method, and apparatus for delivering genetic material to a shoot apical meristem. The delivery system may have a biolistic particle delivery assembly having a gas acceleration tube and a bombardment chamber. The bombardment chamber has a microcarrier launch assembly comprising an opening adapted to receive gas for acceleration of the microcarrier system and to direct gas to a target. The delivery system may have a source of pressurized carrier gas operatively connected to a first end of the gas acceleration tube; and an extender. The extender has a channel configured to fit through the opening of the microcarrier launch assembly. The length of the extender facilitates the penetration of the microcarrier system to an L2 layer of the shoot apical meristem, conferring germline transformation and / or modification.

This invention belongs to the field of biomedical engineering technology, specifically the field of cell function detection technology, and relates to a method for evaluating the ability of mesenchymal stem cells (MSCs) to promote Treg cell proliferation. This method utilizes porous microcarriers to achieve three-dimensional cell distribution, employing low-speed rotation throughout the culture process to simulate the in vivo suspension microenvironment. A culture medium matching human physiological parameters is configured, with oxygen concentration adjusted to a hypoxic range of 1%-3% to simulate the microenvironment of inflammatory sites. Mononuclear cells with a purity of over 95% are obtained through magnetic bead sorting and co-cultured with MSCs for approximately 3 days, significantly shortening the culture period and reducing data deviation, thus achieving specific screening of MSCs with high immunomodulatory functions. By constructing a co-culture microenvironment that conforms to human physiological characteristics, this invention effectively improves detection sensitivity and repeatability, avoids interference from individual differences in peripheral blood mononuclear cells from different sources, and provides technical support for the quality control and clinical application of MSCs.

The application discloses a kind of double-network amphiphilic hydrogel microcarriers capable of maintaining cell stemness and application thereof, and belongs to the technical field of cell culture.The present application copolymerizes SBMA with acrylamide (AAm) monomer to form a double-network structure with acrylic acid (PAA) to prepare hydrogel microcarriers.The double-network structure hydrogel microcarriers PSAm obtained based on amphiphilic materials can effectively expand MSCs while maintaining their cell stemness for a long time, which is conducive to long-term culture of MSCs.Compared with ordinary culture, the artificial subculture process can be reduced, manpower and material resources can be saved, resource waste can be avoided, and a new experience method is provided for long-term culture, preservation and maintenance of cell stemness of MSCs.

The invention discloses a method for large-scale cryopreservation of animal cells. The method comprises the following steps: carrying out first-stage amplification on to-be-preserved cells under the support of matrigel; performing second-stage amplification on the to-be-preserved cells subjected to the first-stage amplification under the support of a microcarrier; mixing the to-be-preserved cells with a serum-free cryopreservation solution to prepare a micro-capsule suspension; and putting the microcapsule suspension into a cryopreservation container to carry out cryopreservation. According to the invention, through amplification of two stages, the to-be-preserved cells have reached a high density of 109 orders of magnitude before cryopreservation, a re-amplification link in a cell recovery stage can be greatly shortened, and the to-be-preserved cells are subjected to microencapsulation through the micro-fluidic chip and then cryopreserved, so that physical protection is given to the to-be-preserved cells; the damage of ice crystals in the cryopreservation process is reduced, the survival rate after resuscitation is improved, and the microencapsulated cells can also be directly subcultured, so that the resuscitation operation is simplified; according to the cell cryopreservation device, the cryopreservation container with a large volume is used, so that the cost of cell cryopreservation is reduced.

A substrate for culturing cells that comprises a modified prolamin having a net positive charge is provided. The modified prolamin may be a prolamin that has been modified through amidation or esterification, such as the addition of a methyl-ester group or an ethanolamine group. The modified prolamin is animal-free may be food-grade and / or may be free of cross-linkers. The modified prolamin may be in a coating that can be applied to a surface for culturing adherent cells, such as a microcarrier (e.g., beads) or well plates. Substrates coated with the modified prolamin are able to support cell expansion and can be used for food-grade applications such as cultured meat.

The invention provides a composite conditioner for acid soil remediation and application thereof, and belongs to the technical field of agricultural environment and soil remediation. The composite conditioner comprises a first component, a second component and a third component which are independently packaged, wherein the modified straw biochar in the first component is used as a'microcarrier 'and a'buffer', calcium magnesium ore powder containing high-activity calcium magnesium oxide is loaded, slow release of alkaline substances is achieved, local over-alkalinity and hardening risks are avoided, and the porosity and the fertilizer retention capacity are improved; the second component is compounded with high-molecular polymer hydrolytic polymaleic anhydride, polyaspartic acid and polyglutamic acid according to a specific proportion, and the synergistic chelation capacity to aluminum and manganese ions and the dispersion capacity to soil particles are obviously superior to those of any single component and other proportion combinations; the third component selects an acid-resistant compound strain and is supplemented with amino acid to serve as a nutrient substance for rapid proliferation of the acid-resistant compound strain, so that high survival rate, colonization success rate and functional expression efficiency of the exogenous flora in an acidic stress are ensured.

The invention discloses a POCT (Point of Care Testing) microfluidic detection chip carrying a photonic crystal structure color coding microcarrier and a preparation method of the POCT microfluidic detection chip. The POCT chip comprises a high-definition and high-transparency cover glass used for packaging and visual detection and a chip main body used for placing the photonic crystal structure color coding microcarrier. And the liquid inlets on the chip main body are uniformly dispersed into the reaction chamber of the chip through the multi-stage flow channel. The reaction chamber comprises a plurality of limiting columns which can separate different microcarriers, so that each microcarrier can be in uniform contact with reaction liquid. After the microcarrier is placed on the chip main body in a random mode, the chip main body is packaged by cover glass and biological glue. Due to the fact that the whole chip is subjected to hydrophilic treatment, when an extremely small amount of to-be-detected sample is dropped into the liquid inlet, reaction liquid can rapidly infiltrate the interior of the whole chip and react with the coding microcarrier in the chip. And after incubation for half an hour, washing the residual sample with a buffer solution, and finally reading the result. According to the invention, trace unknown samples can be detected in a short time, and development of on-site rapid detection can be promoted.

The invention relates to a cell attachment peptide containing an RGD amino acid motif, characterized by a material-side motif derived from poly-L-lysine and a cell-side motif derived from vitronectin. The peptide may be used to functionalize materials for use in cell cultures, such as microcarriers, scaffolds, and substrates.

The present disclosure relates to a microcarrier for cell culture comprising polystyrene-based particles, a method for preparing the microcarrier for cell culture, and a cell culture composition using the same.

A method for forming microcarriers includes forming liquid drops from a sol-gel solution; depositing the drops in the form of a liquid on a first, preferably hydrophobic support; deforming the drops deposited on the first support; solidifying the drops by gelling and drying, so as to form solid microcarriers; and extracting the solidified microcarriers from the first support.

The present application relates to a kind of microcarrier and its application, the microcarrier has three-dimensional structure, the microcarrier has shell and inside is hollow or solid, the shell outer wall is uneven and has multiple concave points.This microcarrier improves the permeability of traditional microcarrier, is conducive to the material exchange between cell and external environment, improves cell survival rate, provides good mechanical protection for cell proliferation and constructs good three-dimensional structure for cell growth, realizes the friendly connection of cell, is conducive to the large-scale expansion of cell in limited space, and maintains the stability of cell physicochemical properties in long-term culture.

The invention provides a microcarrier freeze-dried bead and a preparation method thereof. The microcarrier freeze-dried beads are prepared by the following steps: uniformly mixing a wet microcarrier with an excipient, dispersing and dripping the mixture into liquid nitrogen, quickly freezing into small solid round ice balls with uniform and regular forms, and then freeze-drying to form the freeze-dried beads. The freeze-dried bead is uniform in particle size and round and full in shape, one freeze-dried bead contains 150-4000 microcarriers, the freeze-dried bead has a loose net structure and is quick to redissolve, and the redissolved microcarriers are good in dispersity. The form of the microcarrier freeze-dried beads changes the conventional form of freeze-dried powder of the microcarrier, and basically eliminates the electrostatic adsorption phenomenon of the microcarrier freeze-dried powder, and solves the problems that the microcarrier freeze-dried powder is difficult to weigh, easy to fly and pollute, difficult to subpackage and the like. A certain amount of microcarriers are contained in a single freeze-dried bead, so that quantitative use is facilitated. A freeze-dried bead skeleton structure formed by the excipient formula has dispersion and protection effects on the microcarrier, so that the freeze-dried beads cannot be separated during transportation, are quickly redissolved and dispersed during use, and can be subsequently used for cell culture.

The present invention relates to a filter bag assembly comprising a flexible bag delimiting a compartment configured to contain a fluid. An inlet port and an outlet port are each secured to the flexible bag so as to communicate with the compartment. A porous filter sheet is disposed within the compartment of the flexible bag such that fluid entering the compartment through the inlet port must pass through the filter sheet before exiting the compartment through the outlet port. A first retention seal secures the porous filter sheet to a portion of the flexible bag within the compartment, the first retention seal having an outer peripheral edge forming an annular continuous loop.

This invention discloses a method for large-scale expansion of MSC cells using 3D culture, comprising the following steps: MSC seed cell resuscitation; MSC seed cell planar culture; MSC seed cell planar expansion; MSC seed cell concentration and washing; primary scale-up culture of MSC seed cells; MSC cell washing and concentration; MSC seed cell scale-up culture; microcarrier lysis and cell suspension collection; MSC cell washing and concentration; MSC cell aliquoting and cryopreservation. The advantages of this invention are: it achieves highly efficient scale-up of MSC cells from 2D planar culture to a 3D microcarrier bioreactor, enabling cell expansion to reach more than 20-fold (primary scale-up culture) to more than 200-fold (secondary scale-up culture), while maintaining cell viability >95%.

This application discloses a hepatocyte spheroid, a hepatocyte spheroid formulation, its preparation method, and its application. Hepatic progenitor cells are seeded onto microcarriers and cultured in 3D under oxygen conditions. The microcarriers are then degraded using digestive enzymes to obtain hepatocyte progenitor cell clusters. These clusters are then cultured in a 3D reactor with stirring, and induced to differentiate in serum-free hepatocyte culture medium to obtain hepatocyte spheroids. The hepatocyte progenitor cell clusters of this application are cryopreserved and spontaneously spheroidize upon thawing before use. The induction into a hepatocyte spheroid formulation takes only about one week, resulting in higher spheroidization efficiency, less cell loss, and higher thawing viability, meeting clinical treatment needs. Fresh formulations can be directly used for the clinical treatment of various chronic, chronic-onset, and acute liver diseases. Compared to single cells, these spheroids exhibit longer and more stable survival periods in vivo and in vitro, as well as better storage, transportation, and usage stability. Preparation is also simpler, requiring no cell digestion or other procedures.

The utility model discloses microsphere and microcarrier continuous generation equipment which comprises two supporting plates, the upper surfaces of the two supporting plates are both fixedly connected with heat insulation plates, the inner surfaces of the heat insulation plates are fixedly connected with electrode connecting columns, the electrode connecting columns are fixedly connected with heating blocks, and the heating blocks are fixedly connected with heating wires. Four fixing blocks are fixedly connected to the upper surface of the heat insulation plate, a mounting plate is fixedly connected to the upper surfaces of the four fixing blocks, a mounting groove is formed in the mounting plate, an electromagnetic valve is fixedly connected to the upper surface of the mounting plate, and a connecting pipe is fixedly connected to the input end of the electromagnetic valve; and the output end of the electromagnetic valve is fixedly connected with an instillation head, the instillation head is located in the mounting groove, and the lower surface of the instillation head is fixedly connected with a splash-proof lengthened pipe. Heat generated by the heating block is effectively prevented from being disorderly dissipated to the environment through the heat insulation plate, energy consumption in long-term operation is reduced, and the sustainability of equipment operation is guaranteed from the energy utilization link.

In cell culture, the microcarriers are brought into contact with cells adhering to the bottom surface of a first culture substrate having a bottom surface treated for cell adhesion.

To provide a novel method for producing induced pluripotent stem cells (iPS cells) employing a novel scaffold material that can reduce problems associated with conventional microcarriers, and a method for producing differentiated cells using the iPS cells obtained by the production method.SOLUTION: Somatic cells are brought into contact with reprogramming factors, and induced pluripotent stem cells are established in the presence of a sheet-like piece having a surface containing a polymer material as at least one principal surface.SELECTED DRAWING: Figure 1

The invention relates to an elastic inorganic hydroxyapatite nanofiber microsphere as well as a preparation method and application thereof. The elastic inorganic hydroxyapatite nanofiber microspheres are composed of hydroxyapatite nanowires and have pore structures which are communicated with one another. The elastic inorganic hydroxyapatite nanofiber microsphere provided by the invention has good stem cell attachment and proliferation capacities, does not contain any organic component, has no endotoxin pollution risk, is easy to store and disinfect, is a novel cell microcarrier material, and has good application prospects. Wide application prospects are realized in the fields of drug delivery, medical treatment, medical beauty filling, scientific research and the like.