Method used for preparing microcarrier/polymer composite scaffold by electro-deposition

Abstract

The invention relates to a method used for preparing a microcarrier / polymer composite scaffold by electro-deposition. The method comprises following steps: the surface of a microcarrier is modified, so that the surface of the microcarrier is positively charged; the microcarrier is loaded with a bioactive component so as to obtain the functional microcarrier; the functional microcarrier is delivered into an organic solvent, and the mixture is treated by ultrasonic and is stirred so as to obtain an electro-deposition solution with a concentration of 0.1 to 1.0mg / ml; a polymer scaffold is prepared, an electrode provided with the polymer scaffold is taken as a cathode, and a blank electrode is taken as an anode; the cathode and the anode are delivered into the electro-deposition solution, and the electro-deposition solution is stirred for electro-deposition so as to obtain the composite scaffold; and the composite scaffold is washed and dried in the air so as to obtain the microcarrier / polymer composite scaffold. Preparation time is short; reaction conditions are mild; and it is impossible to cause early release or inactivation of the bioactive component loaded on the microcarrier. The microcarrier / polymer composite scaffold is capable of simulating the multiple interaction of cells in the body, ECM and growth factors, and providing an ideal environment for tissue therapy and tissue repair.

Description

technical field [0001] The invention belongs to the field of preparation of composite supports, in particular to a method for preparing microcarrier / polymer composite supports by using electrodeposition technology. Background technique [0002] In the process of tissue repair, the scaffold plays a vital role in supporting cell growth, guiding tissue regeneration, controlling tissue structure and releasing active factors, and is one of the key factors determining its success or failure. Functional modification of polymer scaffolds, such as compounding some bioactive components such as cell growth regulators, drugs, and enzymes, can promote the repair of damaged tissues. [0003] Since these biologically active components are easily denatured, and excessive release may cause toxicity and carcinogenicity, the use of effective carriers is a necessary condition for realizing their biological functions. Commonly used microcarriers include mesoporous silica, hydroxyapatite, carbon...

AI Technical Summary

Problems solved by technology

One method is to prepare microcarriers on the surface or inside of the polymer matrix by in-situ composite method, such as Masanori et al. (Biomaterials2001; 22:1705-11.) 2 , H 3 PO 4 And collagen as raw materials, control the reaction temperature, pH of the solution, carry out co-precipitation reaction, synthesize hydroxyapatite (HA) / collagen complex, but this method is difficult to load bioactive components in microcarriers at the same time

Another method is to prepare microcarriers first, and then compound them with polymer scaffolds, such as Li et al. (J Mater Sci-Mater M2011;23:547-54.) adding HA directly to polycaprolactone (PCL) solution Among them, the HA / PCL composite scaffold was prepared by electrospinning technology, but this method is easy to inactivate the loaded bioactive components under high pressure conditions, thus affecting its biological properties

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