Method used for preparing microcarrier/polymer composite scaffold by electro-deposition

A composite scaffold and microcarrier technology, applied in prosthetics, medical science, etc., can solve the problems of inactivation of bioactive components, load of bioactive components, affecting biological performance, etc., and achieve the effect of short preparation time and mild conditions

Inactive Publication Date: 2013-11-27
DONGHUA UNIV
View PDF9 Cites 37 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One method is to prepare microcarriers on the surface or inside of the polymer matrix by in-situ composite method, such as Masanori et al. (Biomaterials2001; 22:1705-11.) 2 , H 3 PO 4 And collagen as raw materials, control the reaction temperature, pH of the solution, carry out co-precipitation reaction, synthesize hydroxyapatite (HA) / collagen complex, but this method is difficult to load bioactive components in microcarriers at the s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method used for preparing microcarrier/polymer composite scaffold by electro-deposition
  • Method used for preparing microcarrier/polymer composite scaffold by electro-deposition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of mesoporous silica nanoparticles, which are aminated and loaded with dexamethasone;

[0028] (2) PLLA / PCL70:30 three-dimensional porous nanofibrous scaffold was prepared by thermal phase separation technology: PLLA:PCL was dissolved in tetrahydrofuran with a mass ratio of 70:30, heated and stirred to form a homogeneous solution with a concentration of 10wt%; Then inject it into the syringe, and place it at -80°C quickly to cause the phase separation process to occur overnight; after taking it out, return the syringe, soak the gelled polymer in deionized ice water for 2 days, and change the solvent every day Three times of deionized ice water; after freeze-drying, a three-dimensional nanofiber scaffold with a porous structure was obtained.

[0029] (3) Weigh 50 mg of the mesoporous silicon loaded with dexamethasone prepared in (1), dissolve it in 200 mL of absolute ethanol, ultrasonicate and stir to obtain a uniform electrolyte;

[0030] (4) Fix the th...

Embodiment 2

[0032] (1) Prepare chitosan microspheres and load bone morphogenetic protein;

[0033] (2) Preparation of PLLA nanofiber membrane by electrospinning technology: PLLA was dissolved in a mixed solution of dichloromethane and N,N-dimethylformamide with a volume of 9:1 to obtain a 10wt% electrospinning solution; 18G The injection needle is a spinneret, and the nanofiber membrane is received under the conditions of a voltage of 10kV, a receiving distance of 15cm, and a liquid supply rate of 1mL / h.

[0034] (3) Weigh 20 mg of chitosan microspheres loaded with bone morphogenetic protein prepared in (1), dissolve in 200 mL of absolute ethanol, ultrasonicate and stir to obtain a uniform electrolyte;

[0035] (4) Fix the nanofiber membrane scaffold prepared in (2) on the stainless steel electrode as the working electrode, the blank platinum electrode as the counter electrode, and the calomel electrode as the reference electrode, and place them together in the electrolyte solution in (3)...

Embodiment 3

[0037] (1) Prepare PLGA microspheres, use chitosan for surface modification, and load sphingosine phosphate;

[0038] (2) Collagen nanofiber membrane was prepared by electrospinning technology; collagen was dissolved in hexafluoroisopropanol to obtain 8wt% electrospinning solution; 18G injection needle was used as the spinneret, and the voltage was 16-18kV, accepted The nanofibrous membrane was received under the condition that the distance was 15cm and the liquid supply rate was 0.8mL / h.

[0039] (3) Weigh 30 mg of PLGA microspheres loaded with sphingosine phosphate prepared in (1), dissolve in 200 mL of isopropanol, ultrasonicate and stir to obtain a uniform electrolyte;

[0040] (4) Fix the nanofiber membrane support prepared in (2) on the stainless steel electrode as the working electrode, the blank titanium electrode as the counter electrode, and the calomel electrode as the reference electrode, and place them together in the electrolyte in (3). Electrochemical depositio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method used for preparing a microcarrier/polymer composite scaffold by electro-deposition. The method comprises following steps: the surface of a microcarrier is modified, so that the surface of the microcarrier is positively charged; the microcarrier is loaded with a bioactive component so as to obtain the functional microcarrier; the functional microcarrier is delivered into an organic solvent, and the mixture is treated by ultrasonic and is stirred so as to obtain an electro-deposition solution with a concentration of 0.1 to 1.0mg/ml; a polymer scaffold is prepared, an electrode provided with the polymer scaffold is taken as a cathode, and a blank electrode is taken as an anode; the cathode and the anode are delivered into the electro-deposition solution, and the electro-deposition solution is stirred for electro-deposition so as to obtain the composite scaffold; and the composite scaffold is washed and dried in the air so as to obtain the microcarrier/polymer composite scaffold. Preparation time is short; reaction conditions are mild; and it is impossible to cause early release or inactivation of the bioactive component loaded on the microcarrier. The microcarrier/polymer composite scaffold is capable of simulating the multiple interaction of cells in the body, ECM and growth factors, and providing an ideal environment for tissue therapy and tissue repair.

Description

technical field [0001] The invention belongs to the field of preparation of composite supports, in particular to a method for preparing microcarrier / polymer composite supports by using electrodeposition technology. Background technique [0002] In the process of tissue repair, the scaffold plays a vital role in supporting cell growth, guiding tissue regeneration, controlling tissue structure and releasing active factors, and is one of the key factors determining its success or failure. Functional modification of polymer scaffolds, such as compounding some bioactive components such as cell growth regulators, drugs, and enzymes, can promote the repair of damaged tissues. [0003] Since these biologically active components are easily denatured, and excessive release may cause toxicity and carcinogenicity, the use of effective carriers is a necessary condition for realizing their biological functions. Commonly used microcarriers include mesoporous silica, hydroxyapatite, carbon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/54A61L27/02A61L27/18A61L27/20A61L27/24
Inventor 何创龙仇可新王伟忠冯炜王红声莫秀梅
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap