Novel extraction method of momordica saponins

A kind of balsam pear saponin and extraction method technology, applied in the new extraction field of balsam pear saponin, can solve the problems of low active pharmaceutical ingredients, residual organic solvent, low yield of target product, etc., and achieve low cost, mild reaction conditions and increased yield Effect

Active Publication Date: 2016-10-12
SHANTOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a novel extraction method of bitter melon saponins, increase the yield of total bitter melon saponins through the combination of a novel two-phase system and a macroporous resin separation method, and solve the problem of organic solvent residues in existing extraction methods and low drug active ingredients , problems such as low yield of target product

Method used

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  • Novel extraction method of momordica saponins
  • Novel extraction method of momordica saponins
  • Novel extraction method of momordica saponins

Examples

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Effect test

Embodiment 1

[0035] Example 1 Preparation of Momordica charantia saponin using three different extraction methods

[0036] S1 Preparation of Momordica charantia saponin extract A: Take 55g of dried Momordica charantia powder and dissolve it in 70% ethanol at a solid-to-liquid ratio of 1:20 (g / mL), shake it well, place it in a 60℃ water bath, and take it out after 1 hour Centrifuge at 4000 rpm for 10 min, and combine the supernatants to obtain an alcohol extract of momordica charantia L. and concentrate it in a rotary evaporator until the concentrated solution has no alcohol taste to prepare a momordica saponin extract A.

[0037] S2 Preparation of Momordica charantia saponin extract B1: using ethanol / (NH 4 ) 2 SO 4 Extraction of Momordica charantia saponins alcohol extract A by aqueous two-phase system, the conditions of aqueous two-phase extraction are as follows: Tether length (TLL) is 45, compared (R) is 2.5, and the mass fraction of each component is 45.195% ethanol and 33.594% ( NH 4 ) 2 S...

Embodiment 2

[0042] Example 2 Evaluation of indicators for in vitro hypoglycemic activity

[0043] The samples in this embodiment are acarbose and momordica saponins D1, D2, and D3. After purification of Momordica charantia saponins extract A and B1, the eluates with an ethanol concentration of 60-70% are collected, and the ethanol is removed by rotary evaporation to prepare refined Momordica charantia saponins C1 and C2. Momordica charantia saponin extract B2 is purified and the ethanol is collected The eluate with a concentration of 72-82% is rotary evaporated to remove the ethanol to prepare the refined extract of Momordica charantia saponins C3. The finished products of Momordica charantia saponins D1, D2 and D3 were prepared by freeze-drying. Momordica charantia saponins in vitro hypoglycemic activity test indicators are evaluated by its inhibition rate of α-glucosidase activity.

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Abstract

The invention relates to the technical field of natural product deep processing, in particular to a novel extraction method of momordica saponins. The novel extraction method comprises the following steps that 1, dry bitter gourd powder is taken to be dissolved into ethyl alcohol, oscillating and shaking are conducted to enable the mixture to be uniform, the mixture is put into a 60-DEG C water bath kettle to be extracted for 1 hour and then centrifuged to obtain a momordica saponins alcohol extracting solution, the momordica saponins alcohol extracting solution is subjected to vacuum concentration in a rotary evaporator until a concentrated solution is free of alcohol smell, and then a momordica saponins extracting solution A is prepared; 2, extraction is conducted by adopting an isopropyl alcohol-(NH4)2SO4 aqueous two-phase system, after partition equilibrium is achieved, phase separation is conducted, an upper phase is take, and a momordica saponins extracting solution B is prepared; 3, the crude momordica saponins extracting solution B is further purified through AB-8 type macroporous adsorption resin, and a fine momordica saponins extract C is prepared; 4, freeze drying is conducted to prepare the finished momordica saponins. Compared with a traditional method, the momordica saponins prepared through ethanol extraction, aqueous two-phase extraction and macroporous resin separation has the advantages that the yield of the momordica saponins is increased, and the blood glucose reducing activity of the momordica saponins is also obviously improved.

Description

Technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a novel extraction method of momordica saponins. Background technique [0002] Momordica charantia belongs to the genus Momordica in the Cucurbitaceae family. It is a natural plant with "dual use for medicine and food". Momordica charantia contains various chemical components such as saponins, polysaccharides, proteins and peptides. Momordica charantia saponins are a kind of more complex glycoside compounds, also known as glycosides. They are compounds formed by connecting sugar or sugar derivatives with another non-sugar substance through its terminal carbon atom. Sapogenin is composed of sugar, uronic acid or other organic acids. According to the chemical structure of aglycon, it can be divided into two categories: triterpene saponins and steroidal saponins. According to reports, it has the functions of anti-tumor, improving immunity, lowering blood suga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 刘杨李明莉肖湘
Owner SHANTOU UNIV
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