87results about How to "Simple extraction process" patented technology

The invention provides a method for efficiently extracting light-color lignin and application. The method comprises the steps that bamboo is smashed, bamboo powder is screened out and dried, then formic acid is used for pretreatment and filtration to obtain solid residues, deionized water is used for washing the solid residues to be neutral, and solid matter is obtained after drying; treating the solid substance by using a tetrahydrofurfuryl alcohol / p-toluenesulfonic acid aqueous solution system, filtering out insoluble impurities to obtain filtrate, washing the insoluble impurities by using a tetrahydrofurfuryl alcohol aqueous solution and deionized water until the insoluble impurities are neutral, and reserving a washing solution; and adding water into the filtrate and the washing liquid for precipitation to obtain light-color lignin. According to the method for extracting the lignin, the yield is 66.9%, the purity is 99.64%, the obtained lignin is light in color (lightness: 70.48), the molecular weight of the obtained lignin is moderate (Mw: 4695), and the negative influence of the extraction process on lignin structural units is limited.

The invention provides a process for extracting fresh peony flower cell water from fresh peony flowers. The extraction process comprises the following steps: A, firstly, picking fresh peony flowers; b, the picked peony flowers are pretreated; c, performing shearing treatment on the pretreated peony flowers; d, the sheared peony flowers are added into a presser to be pressed, and pressed residues and filtrate are obtained; e, adding the pressure slag into a distillation retort for primary distillation treatment, and filtering to obtain primary distillate; f, mixing the filtrate and the primary distillate, adding the mixture into a reaction kettle, and adding enzymatic hydrolysate for enzymolysis treatment to obtain a primary enzymolysis solution; and G, filtering the primary enzymolysis solution, adding the filtered solution into a distillation retort, carrying out secondary distillation treatment, and filtering to obtain a secondary distillate. The extracted cell water does not contain impurity ions and harmful substances, spice components and thermosensitive substances in the peony petals are reserved, and the activity of cosmetics can be effectively improved when the cell water is added into the cosmetics.

The invention discloses deer myocardial peptide capable of replenishing qi and enriching blood as well as a preparation method and application of the deer myocardial peptide, and relates to the technical field of functional foods. The preparation method comprises the following steps: uniformly mixing fresh deer heart and a fermentation composition, and fermenting to obtain fermentation liquor; homogenizing the fermentation liquor to obtain homogenized liquor; mixing the homogenized liquid with compound enzyme, and performing enzymolysis to obtain enzymatic hydrolysate; and carrying out ultrafiltration concentration on the enzymatic hydrolysate, and drying to obtain the deer myocardial peptide. The micromolecular deer myocardial peptide prepared by the invention is high in purity and high in activity, and shows a good improvement effect in the aspects of replenishing qi and enriching blood, so that the application of the deer myocardial peptide is expanded.

According to the application, the excellent insect-resistant activity of the zingiberaceae plant extract on pests such as agricultural mites and thrips and the compounding synergistic and co-extraction synergistic relationship among different species of ginger extracts are found for the first time, the application range of the ginger extract is expanded, and a new choice is also provided for controlling the mites and the thrips in agriculture.

The invention belongs to the technical field of agriculture, and particularly relates to application of an astragalus extract containing astragalus polysaccharide in promoting plant growth. Purified astragalus polysaccharide is proved to have good growth regulation and stress resistance enhancement functions on plants, but the extraction and purification process of the polysaccharide is high in cost and low in efficiency, the requirement of large-scale production is difficult to meet, and the application of the astragalus polysaccharide in agriculture is limited. The invention provides an application of an astragalus extract containing astragalus polysaccharide, and particularly relates to an application of the astragalus extract as a plant growth promoter for promoting plant growth and improving stress resistance of plants, reducing the use concentration of the astragalus polysaccharide, promoting the application of the astragalus polysaccharide in agriculture, and providing a new choice for regulating and controlling plant growth in agriculture.

The invention relates to the technical field of diglyceride oil, and discloses a diglyceride-containing grease composition and a preparation method thereof. According to the present invention, a variety of vegetable oils are combined, the combined vegetable oil is subjected to glycerol diesterification, and the turmeric is directly extracted by using the oil after the enzymolysis so as to integrally prepare the curcumin-containing diglyceride oil; according to the present invention, the mass content of diglyceride in the diglyceride oil prepared by combining a plurality of vegetable oils is 40-95%, the mass content of oryzanol is 10000-20000 ppm, the mass content of sterol is 8000-10000 ppm, and the mass content of curcumin is 0.1-0.2%, such that the diglyceride, the oryzanol, the sterol and the curcumin in the diglyceride oil are subjected to a specific content and proportion so as to prevent the color reversion of the oil composition, such that the color reversion of the oil composition can be prevented, and the oil composition can be easily obtained. The grease composition keeps bright appearance and has the advantage of being good in stability.

The invention belongs to the field of pharmaceutical analysis, and particularly relates to a method for establishing a traditional Chinese medicine fingerprint spectrum of a gastrodia elata and uncaria decoction. The method is realized by the following steps: firstly, preparing a reference substance solution and a test solution, then detecting by adopting a high performance liquid chromatography, and establishing a fingerprint spectrum. According to the method provided by the invention, by reasonably selecting the components of the reference substance and adopting a multi-wavelength method according to the maximum absorption wavelength of the components, the change of the component amount between the samples can be effectively represented, so that the quality of the samples is more accurately judged; the invention further provides a screening and optimizing method for the preparation method of the gastrodia elata and uncaria decoction, the prepared method is good in repeatability and stability, the quality of the gastrodia elata and uncaria decoction can be monitored more comprehensively, and the method provides valuable reference for quality evaluation and standardization of traditional Chinese medicine decoction.

The invention discloses a plant cell nucleus extraction method and application, and belongs to the technical field of plant single cell sequencing. The method comprises the following steps: carrying out tissue immobilization on plant tissues by using precooled methanol; preparing a cell nucleus lysis solution, namely an NIB solution; cleaning and chopping the fixed tissue sample, and incubating the tissue sample in an NIB solution; filtering with a screen; centrifuging, discarding supernatant, and resuspending and precipitating the NIB solution to obtain a suspension; slowly dripping the suspension into a Percoll solution, and carrying out density gradient centrifugation to purify cell nucleuses. The invention provides a high-applicability and high-quality cell nucleus extraction method which is suitable for cryopreserved or fresh tissue samples, higher cell nucleus yield and mRNA capture rate are obtained, and the data quality of single cell sequencing is improved.

The invention relates to a multi-modal knowledge representation method oriented to information compliance management. The method comprises the following steps: firstly, extracting visual modal features, considering the efficiency problem encountered by using a convolution-free image segmentation method to standardize and segment an image by using a convolution-free image segmentation method, and then obtaining image data features through projection; extracting a subject term from the long text through a KeyBert model, encoding the text by using MacBert, performing semantic substitution on the original text through position information of the subject term contained in the text, and obtaining a text modal feature in combination with a type feature of the original text; and finally, performing multi-modal fusion on the two modal features by using a BiLSTM-Attention model to realize semantic representation of government affair field information. According to the method, the efficiency of visual modal feature extraction can be improved, text modal feature extraction oriented to long text data is realized, and effective representation of government affair field information is completed through the fusion model, so that government affair information is further discriminated.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a foundation-consolidating and anti-aging traditional Chinese medicine composition and a preparation method thereof. The traditional Chinese medicine composition is prepared from corn stigma, semen cuscutae, oysters, dogwood, gordon euryale seeds, white poria cocos, radix asparagi, lotus seeds, polygala tenuifolia pulp, ophiopogon roots, prepared rehmannia roots, schisandra chinensis, radix morindae officinalis, semen plantaginis, common yam rhizomes, fructus lycii, radix codonopsis, costus roots, broussonetia papyrifera leaves, platycladi seeds, raspberries, Chinese dates, Chinese yams, ginseng, codonopsis pilosula and salvia miltiorrhiza. The aging process can be slowed down, and the service life can be prolonged.

The invention discloses a servo motor temperature field simulation method and device, and the method comprises the steps: carrying out the grid division of a servo motor, building a conjugate heat transfer model, including fluid domain grid modeling and solid domain grid modeling, carrying out the simulation operation of the fluid domain grid modeling, obtaining the flow field information of the servo motor, and carrying out the simulation operation of the solid domain grid modeling, the flow field information of the servo motor is mapped to the fluid domain grid modeling to serve as initial flow field information, the initial flow field information is kept unchanged in the simulation process, and according to the actual operation working condition and the heat conduction model of the servo motor, the flow field information of the servo motor is mapped to the fluid domain grid modeling to serve as the initial flow field information. And generating simulation results of the fluid temperature field and the solid temperature field of the servo motor. According to the method, the conjugate heat transfer modeling of the servo motor is constructed, so that the fluid temperature field and the solid temperature field of the servo motor can be subjected to real-time coupling solution, and the accuracy and the efficiency of servo motor temperature field simulation calculation are greatly improved.

The invention relates to a finger-pressing type nucleic acid detection micro-fluidic chip, which comprises a bottom layer, a flow channel layer, a finger-pressing layer and a thin film layer, the flow channel layer comprises an LAMP reaction chamber, a CRISPR reaction chamber, a water storage chamber, a Tesla valve, a finger-pressing chamber and a test paper reaction chamber, the LAMP reaction chamber comprises a nucleic acid extraction-free reaction system based on LAMP, the CRISPR reaction chamber comprises a CRISPR-based cas12b reaction system, and the test paper reaction chamber comprises a Tesla valve, a finger-pressing chamber and a test paper reaction system. The water storage chamber is mainly used for diluting a mixed system, and the Tesla valve is used for preventing crosstalk of the two reaction systems; the finger pressing layer comprises an LAMP reaction chamber button and a CRISPR reaction chamber button, when the LAMP reaction chamber button is pressed, liquid in the LAMP reaction chamber is pumped into the CRISPR reaction chamber, when the two buttons are pressed at the same time, a reaction mixing system is pumped into the water storage chamber to be mixed, and then the reaction mixing system is further pumped into the test paper reaction chamber. The chip can be widely applied to the field of rapid diagnosis of nucleic acid molecules, the detection cost is reduced, manual operation is simplified, and the detection efficiency is improved.

The invention belongs to the technical field of food processing, and particularly relates to an extraction process of turnip seed oil and an effective component composition of the turnip seed oil. The invention relates to an extraction process of turnip seed oil, which comprises the following steps: performing staged fermentation pretreatment on turnip seed broken cell walls by using a plurality of microbial strains, and then extracting grease by combining with a nano SiO2 (at) SDS composite active agent and ionic liquid synergistic ultrasonic treatment. Through staged fermentation pretreatment and the synergistic effect of the nano material and the ionic liquid, the extraction efficiency and quality of the turnip seed oil are greatly improved; firstly, cell walls and internal components are gradually degraded by utilizing aspergillus niger, aspergillus oryzae, bacillus licheniformis, lactic acid bacteria and saccharomycetes, grease is released, and the stability of the grease is improved; then, the extraction process is further optimized by a composite active agent formed by nano silicon dioxide and lauryl sodium sulfate and 1-butyl-3-methylimidazolium acetate, and the efficiency is enhanced through ultrasonic treatment;

The application discloses a use of a quaternary deep eutectic solvent of zinc chloride tetrahydrate and phosphoric acid, the quaternary deep eutectic solvent of zinc chloride tetrahydrate and phosphoric acid is composed of zinc chloride as a hydrogen bond acceptor, phosphoric acid as a hydrogen bond donor and water as a solvation regulator; the quaternary deep eutectic solvent of zinc chloride tetrahydrate and phosphoric acid is used for depolymerizing lignin to prepare small molecule lignin; the quaternary deep eutectic solvent of zinc chloride tetrahydrate and phosphoric acid of the application utilizes Lewis acid and Bronsted acid to synergistically catalyze, efficiently depolymerizes lignin at 60-105 DEG C, and targets to cut off aryl ether bonds; the quaternary deep eutectic solvent has low viscosity and good fluidity, meanwhile, water molecules play a nucleophilic quenching role, and inhibit secondary condensation reaction; after the depolymerization reaction is completed, high-activity small molecule lignin can be obtained through simple water addition and precipitation; the solvent can be recycled; washing liquid can be closed-loop circulated, zero wastewater discharge is realized; the obtained product has a significantly reduced weight average molecular weight and abundant free phenolic hydroxyl groups, and can replace phenol to directly serve as a raw material to synthesize phenol-formaldehyde resin adhesive and the like.

The invention belongs to the technical field of algal polysaccharide extraction, and discloses a modification extraction method of algal asparagus polysaccharide and a method for preparing a lutein coating by using the algal asparagus polysaccharide. The modified extraction method comprises the following steps: extracting gracilaria lemaneiformis powder with a weak base solution, enabling lactide and polysaccharide to be subjected to grafting reaction through a redox initiation system under the assistance of ultrasonic waves, and then separating out a first polysaccharide component with high emulsibility and a second polysaccharide component with high gel property through graded alcohol precipitation. The two obtained functional components are used for coating xanthophyll, specifically, the first polysaccharide component emulsifies a xanthophyll oil phase to form a pre-emulsion, the second polysaccharide component is compounded with gelatin, a composite coacervate is formed on an oil drop interface by adjusting the pH value, and finally drying and curing are conducted. According to the method, two modified polysaccharides with definite functions can be synchronously obtained, the prepared xanthophyll coating is high in encapsulation efficiency, good in storage and processing stability and excellent in water dispersibility, and the problems that xanthophyll is unstable and difficult to apply are effectively solved.

The invention relates to soluble chitin extracted from natural traditional Chinese medicine maggot and a preparation method thereof.The soluble chitin is obtained by extracting chitin of maggot powder through an alkaline method and then conducting modification through a modifying agent, the modifying agent is selected from one or more of 1, 2-epoxypropane and ethylene oxide, and the modifying agent is selected from one or more of 1, 2-epoxypropane and ethylene oxide. The mass / mol ratio of the maggot powder to the modifier is 1g: (0.05-0.2 mol). Compared with the prior art, the chitin extraction rate is high, the chitin is modified to be water-soluble, and the use occasion of the chitin is greatly improved.

The invention provides a method for rapidly extracting light-color phenolated lignin, and belongs to the field of biomass refining. The method comprises the following steps: uniformly mixing a lignocellulose raw material with a mixed solution of aromatic sulfonic acid and a phenolic compound according to a certain solid-to-liquid ratio, and carrying out ultrasonic treatment at a certain temperature; and after the treatment is completed, carrying out solid-liquid separation, pouring the obtained extracting solution into water to precipitate lignin, and washing and drying to obtain the light-color phenolated lignin. The method disclosed by the invention is simple in process, low in time consumption and high in yield, and the solvent is easy to recycle; the prepared lignin is extremely light in color and high in phenolic hydroxyl group content, retains most of aryl ether bonds, can adjust the content of phenolic hydroxyl groups and methoxyl groups in the lignin to a certain extent, and solves the problems of dark color, few functional groups and low reaction activity of the existing industrial lignin. The method can be used for producing lignin-based functional products without further color reduction and activation treatment.

The invention belongs to the technical field of natural plant active products, and particularly relates to a magnolol clathrate compound for inhibiting vibrio in aquaculture and a preparation and application of the magnolol clathrate compound. Magnolol is high-purity magnolol obtained by separating and purifying an active component extracted from a natural plant raw material mangnolia officinalis through a subcritical process. Molecular clathration is carried out on magnolol by adopting a molecular clathration technology to prepare a magnolol clathrate compound, the magnolol clathrate compound is further prepared into different dosage forms such as powder, granules and a liquid preparation to obtain an anti-vibrio preparation product, and tests prove that the anti-vibrio effect of the preparation product is remarkable. The raw materials are low in price and easy to obtain, the magnolol extraction process is simple, green and environmentally friendly, magnolol is clathrated through the molecular clathration technology, the water solubility and oral utilization rate of magnolol are increased, the prepared preparation can be added into feed, the use of antibiotics is reduced, healthy development of green fishery is promoted, and the economic benefit is increased. And a foundation is laid for development of antibiotic-free aquatic natural plant feed additive system products.

The application discloses a method for extracting sodium glycolate from pesticide wastewater and belongs to the wastewater treatment field. After 2,4-D production wastewater is stirred after pH is adjusted to 3.0, the filtrate is collected by filtration, the pH of the filtrate is adjusted to 8.0, and the concentrated solution is obtained by concentration under reduced pressure. The concentrated solution, modified cyclodextrin and modified cellulose are mixed according to the weight ratio of 100:0.8-1.2:0.1-0.3, the first mixing and stirring are performed, the second mixing and stirring are performed after cooling, the third mixing and stirring are performed after cooling again, and the filter cake is collected. After the filter cake and deionized water are mixed and stirred, the filtrate is collected by filtration, and the sodium glycolate is obtained by vacuum drying of the filtrate. The application solves the problem of co-crystallization of sodium glycolate and sodium chloride, and improves the purity and yield of sodium glycolate.

The invention relates to the technical field of cosmetics, in particular to soothing and sunscreen spray as well as a preparation method and application thereof. The spray is prepared from the following raw materials: cyclopentasiloxane, octyl polymethylsiloxane, polydimethylsiloxane, butanediol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl polyethylene glycol (PEG)-10 tri (trimethylsiloxy) silyl ethyl polydimethylsiloxane, ethylhexyl triazinone, an organic solvent, an organic solvent and a solvent. The composition is prepared from herba centellae extract, radix gentianae extract and water. The spray prepared by the invention has excellent effects of soothing the skin, blocking the sun and preventing the skin from dullness.

The present invention relates to the field of enzyme catalysis technology, in particular to an aspartate β-decarboxylase mutant, whose amino acid sequence is shown in SEQ ID NO: 3, and has higher thermal stability than wild-type aspartate β-decarboxylase. The aspartate β-decarboxylase mutant of the present invention has a more lasting activity at 45°C-60°C, which can effectively shorten the reaction cycle; the mutant has low requirements for the cooling system of the reaction, thereby reducing energy consumption and reducing costs. Moreover, it can be transported and stored at room temperature, effectively extending the shelf life. In addition, under the condition of high-temperature catalytic reaction, the growth opportunity of miscellaneous bacteria is avoided, thereby reducing the contamination of the product by bacterial metabolites, helping to increase the solubility of the substrate and improve the production efficiency per unit volume.

The present invention relates to a DNA extraction centrifugal disk. The DNA extraction centrifugal disk comprises at least one DNA extraction unit; the front of the DNA extraction unit is provided with a first liquid inlet, a second liquid inlet, a third liquid inlet, a temporary liquid storage area and a filtration area, and the back is provided with a first liquid storage area and a second liquid storage area; a DNA adsorption material is provided in the filtration area; the first liquid inlet and the second liquid inlet are respectively connected to the filtration area through a flow channel; the third liquid inlet is connected to the temporary liquid storage area through a flow channel, and the temporary liquid storage area is connected to the filtration area through a flow channel; the second liquid storage area is connected to the first liquid storage area through a flow channel, and the first liquid storage area is connected to the filtration area through a flow channel. The DNA adsorption material is preferably a silica gel membrane. The present invention integrates the reaction process of the silica gel membrane adsorption method into the centrifugal disk, which can efficiently realize DNA extraction.

The invention discloses a method for preparing licorice protein from licorice residues. Comprising the steps of licorice protein crude product separation, extraction, refining, decoloration and purification. The liquorice protein is prepared from the liquorice residues, and the method has the advantages of being simple in extraction process and high in operability; and the used solvent and raw materials are easy to buy and have small influence on the environment. The glycyrrhiza uralensis residues are used for preparing the glycyrrhiza uralensis protein to the greatest extent, the prepared glycyrrhiza uralensis protein with the content of 55%-70% meets the requirement of the market for feed protein, and the glycyrrhiza uralensis residues have extremely high market prospects.

The invention belongs to the field of medicine analysis, and particularly relates to a method for establishing a traditional Chinese medicine fingerprint spectrum of a postoperative ventilation decoction. The method is realized by the following steps: firstly, preparing a reference substance solution and a test solution, then detecting by adopting a high performance liquid chromatography, and establishing a fingerprint spectrum. According to the method provided by the invention, by reasonably selecting reference substance components and adopting a timing wavelength method according to the maximum absorption wavelength of the components, the change of the component amount between the samples can be effectively represented, so that the quality of the samples is more accurately judged; the invention further provides a screening and optimizing method for the preparation method of the postoperative ventilation prescription decoction, the prepared method is good in repeatability and stability, the quality of the postoperative ventilation prescription decoction can be monitored more comprehensively, and the method provides valuable reference for quality evaluation and standardization of traditional Chinese medicine decoction.

The present application relates to the field of information processing technology, and provides a table information extraction method, device, equipment and medium, the method comprising: obtaining a table to be processed, performing recognition processing on the table to obtain multiple text blocks, and inputting each text block into a pre-trained named entity recognition model, so as to obtain a semantic label corresponding to a target text block belonging to a content record through the named entity recognition model, the semantic label is used to characterize the table header title corresponding to the target text block, and then according to the semantic labels corresponding to each target text block, determine the target semantic entity corresponding to the semantic label in the table, and finally generate structured data according to the semantic label and the target semantic entity corresponding to the semantic label. The table extraction method provided in this embodiment simplifies the extraction process while improving the extraction accuracy, thereby improving the timeliness of information extraction.

The invention provides an anti-esophageal cancer medicine derived from fresh rabdosia rubescens and a preparation method of the anti-esophageal cancer medicine, and relates to the technical field of pharmacy of fresh traditional Chinese medicines. According to the invention, fresh rabdosia rubescens is taken as a basic medicinal material, an extraction process is improved and optimized, an extract containing Oridonin (Ori) and rosmarinic acid (RA) is obtained, and the esophageal cancer resisting effect of the fresh rabdosia rubescens extract is researched. Experimental results show that the treatment effect of Ori combined with RA administration on esophageal cancer model mice is remarkable, the effect is better than that of Ori or RA independent administration, and it is shown that oridonin and rosmarinic acid are combined for use, and the esophageal cancer resisting effect can be remarkably improved. The invention provides a novel medicine and a novel treatment thought for treating esophageal cancer.

The invention relates to stem cells, in particular to application of a subcellular extract of stem cells in preparation of a medicine for treating liver injury. Experiments prove that the prepared mesenchymal stem cell subcellular extract has a remarkable improvement effect on a carbon tetrachloride induced mouse hepatic fibrosis model in vivo and in vitro. The stem cell subcellular extract prepared by a mechanical disruption method can effectively alleviate the morphological problems such as hepatomegaly and rough surface, and improve the symptoms of hepatocyte injury and collagen deposition. In addition, the subcellular extract of the stem cells can significantly reduce the serum transaminase level of mice with hepatic fibrosis and improve the liver function. Meanwhile, by inhibiting the expression of fibrosis-related genes and inflammation-related genes in the liver, the subcellular extract of the stem cells exerts remarkable anti-fibrosis and anti-inflammatory effects. The umbilical cord mesenchymal stem cell subcell extract prepared by a mechanical crushing process provides a new method and thought for clinical treatment of liver injury.

The present invention belongs to the field of bioengineering, and specifically discloses a new process for purifying β-1,3-glucan from a fermentation broth of a saccharomyces cerevisiae cell; comprising the following steps: 1) preparation of a fermentation broth of saccharomyces cerevisiae, 2) purification of β-1,3-glucan; in the preparation of the fermentation broth of saccharomyces cerevisiae, the following initial culture medium is used for fermentation tank culture, including: 25-35 g / L of glucose, 5-6.5 g / L of ammonium sulfate, 7-12 g / L of yeast extract powder, 5-8 g / L of arginine, 0.4-0.6 g / L of magnesium sulfate, 4.1-5.1 g / L of disodium hydrogen phosphate, and 0.5-0.7 g / L of potassium dihydrogen phosphate. The new process disclosed in the present invention has few steps, high unit yield of β-1,3-glucan, convenient purification, low preparation cost, and obvious industrial value.

The invention discloses an application of a soft spot grass extract in the preparation of anti-tumor drugs. The preparation method is to take the dry whole grass of the soft spot grass, wash it with water, dry it and cut it into small pieces, extract it with 5-10 times the amount of solvent at constant temperature reflux for 2-4 times, each time for 1-2.5h, combine the extracts, and concentrate under reduced pressure to obtain a total extract; add 1-3 times the mass of distilled water to the obtained total extract for suspension, use an ultrasonic oscillator to assist the suspension, obtain a suspension, then add 2-5 times the volume of petroleum ether to extract 2-4 times, and combine the petroleum ether extracts; the obtained water layer is extracted 2-4 times with 2-5 times the volume of ethyl acetate, and the ethyl acetate extracts are combined, and the solvent is recovered under reduced pressure to obtain a soft spot grass extract. The invention provides a preparation method of a soft spot grass extract, and the extracted extract is applied to the preparation of anti-tumor drugs, the medicinal value of the soft spot grass is developed, and a theoretical basis is provided for the treatment of tumors and the research and development of new drugs.

The present invention belongs to the technical field of methods for providing apigenin 7-O-glucoside, and specifically relates to a method for extracting and purifying apigenin 7-O-glucoside from peony leaves. A method for extracting and purifying apigenin 7-O-glucoside from peony leaves, comprising the following steps: S1. ethanol extraction; S2. extraction; S3. macroporous resin separation; S4. gel column separation; S5. HPLC preparation and purification. The present invention selects an ethanol concentration with higher extraction efficiency for extraction, and the extract is separated by LX-38 macroporous resin and Sephadex LH-20 gel column respectively. The separation process is mainly based on thin layer chromatography identification, and the standard and the sample are developed under the same conditions, and the components with the same ratio shift value are combined, impurities are removed, and the target compound is retained. Finally, apigenin 7-O-glucoside with higher purity is obtained by semi-preparative high performance liquid chromatography.