Inula flower extract, preparation method thereof and cosmetic composition

Abstract

The invention relates to the technical field of cosmetics, in particular to an inula flower extract, a preparation method thereof and a cosmetic composition. The invention relates to an inula flower extract for reducing skin texture (smoothing skin), resisting wrinkles, enriching apple muscle, lifting mandibular line, reducing pore depth and improving skin elasticity, the extract comprises an aqueous extract of inula flower and / or an organic solvent extract, and the volume fraction of the organic solvent is 0.01%-100%. The inula flower extract disclosed by the invention is rich in phenolic acid, flavone, polysaccharide and sesquiterpenoids, and has the effects of reducing the skin texture degree (smoothing the skin), resisting wrinkles, enriching apple muscles, lifting the mandibular line, reducing the pore depth and improving the skin elasticity.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an Inula flower extract, a preparation method thereof and a cosmetic composition. Background Art

[0002] Aging is an inevitable law of the human life process and an eternal and long-term proposition in the skin care industry. Nowadays, with the rapid development of the skin care industry, the speed of product innovation is also getting faster and faster. The progress of science and technology has filled the gap in the research and development of skin care products in my country. More and more materials can be used in the field of skin care products, but people are increasingly favoring natural products in the selection of skin care material sources. Generally speaking, natural products have the special properties of safety, low irritation, and no pollution, which meets the current people's pursuit of a healthy and high-quality life. Therefore, natural products are widely used in skin care products.

[0003] Inula flower is a traditional Chinese herbal medicine of the genus Inula of the Asteraceae family. It is included in the Chinese Pharmacopoeia under the item Inula flower. The dried flower heads are used as medicine. It is bitter, pungent, salty and slightly warm in nature. It enters the lung, spleen, stomach and large intestine meridians. It has the effects of lowering qi, eliminating phlegm, promoting water flow and stopping vomiting. It is mainly used to treat wind-cold cough, phlegm and fluid accumulation, chest tightness, wheezing, coughing and sputum, vomiting and belching, and hardness under the heart. Modern medical research shows that Inula flower has good biological activity in anti-tumor, anti-asthma, anti-fungal, prevention of hepatitis and liver protection, as well as prevention and treatment of diabetes.

[0004] Wrinkles are the most important surface feature of skin aging. With aging and daily life, the skin gradually becomes thinner, the dermal elastic fibers decrease and the muscles relax. The facial expression muscles are attached to the skin. When the skin contracts, wrinkles will appear at right angles to the direction of contraction. Therefore, wrinkles, sagging and collapse of the apple muscles, and sagging of the jawline are also the focus of anti-aging, and their detection and quantitative evaluation are of great research significance. In evaluating the anti-wrinkle, apple muscle plumping and firming effects, the skin wrinkle indicators used are: skin roughness (Ra, Rq, crow's feet), where roughness Ra represents the arithmetic mean of the peak values ​​on the surface contour line of the skin in the tested local area, and Rq represents the square root of the average value of the peak values ​​on the contour line within the entire test length range. The smaller the value, the smoother the skin and the fewer fine lines on the skin; the crow's feet depth represents the severity of crow's feet, and the smaller the value, the smaller the depth of crow's feet; the above can reflect the smooth skin and anti-wrinkle effect of the skin; when observing the plumpness of the apple muscles, the volume of the apple muscles measured by the instrument is used as an indicator. The principle is to calculate the skin morphology volume above the reference plane in the test area. The volume higher than this reference plane is called positive volume. If the volume increases, it means that the local skin is three-dimensional and plump; due to aging and relaxation of the skin, the skin elasticity decreases, the jaw line relaxes, and the angle of the vertical line with the angle of the lip corner increases. When the skin is lifted and tightened, the angle of the vertical line with the lip corner decreases. If the skin is loose, when the skin is adsorbed, the smaller the ratio of the total elastic-plasticity of the rebound part to the total elastic-plasticity of the stretching part, when the skin is firm, this value is closer to 1, and the area within the envelope curve of the test area, the smaller the F4 value, the more the skin can resist suction (skin firmness). Due to aging, skin loosening, collagen loss, gravity and other skin aging, some pores naturally expand. When the skin is firm, the depth of the pores decreases, so the above can reflect the firmness of the skin. In the related technology, when cosmetics need to comprehensively reflect the effects of anti-wrinkle, firming, and lifting apple muscles, they often need to be combined with different raw materials to achieve this, which is relatively costly and the production process is relatively complicated. At the same time, the different components in the raw material composition will also affect the expression of their efficacy, resulting in the actual product efficacy being lower than expected.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] One object of the present invention is to provide an Inula flower extract, which has the effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up apple muscles, lifting jawline, reducing pore depth, and improving skin elasticity.

[0007] Another object of the present invention is to provide a method for preparing the Inula japonica extract, which is simple and easy to implement.

[0008] Another object of the present invention is to provide a use of the Inula fragrans extract as described above or the Inula fragrans extract obtained by the method in preparing a cosmetic composition.

[0009] Another object of the present invention is to provide a cosmetic composition.

[0010] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0011] The invention discloses an Inula flower extract, which comprises a water extract and / or an organic solvent extract of the Inula flower, wherein the volume fraction of the organic solvent is 0.01% to 100%.

[0012] In one embodiment, the organic solvent extract comprises an alcohol compound solution extract.

[0013] In one embodiment, the alcohol compound includes at least one of a monohydric alcohol and a polyhydric alcohol.

[0014] The preparation method of the Inula japonica extract comprises the following steps:

[0015] The first mixed system is obtained by soaking the inula flower in water or an organic solvent with a volume fraction of 0.01% to 100%. The first mixed system is subjected to heat reflux extraction to obtain a first mixed extraction system, and then solid-liquid separation is performed, and the liquid phase is collected to obtain the inula flower extract.

[0016] In one embodiment, the usage ratio of Inula japonica to water or organic solvent is 1 g: (5-50) mL.

[0017] In one embodiment, the soaking treatment time is 20 to 40 minutes.

[0018] In one embodiment, the temperature of the hot reflux extraction is 78-80° C., and the time of the hot reflux extraction is 50-80 min.

[0019] Use of the Inula fragrans extract as described above or the Inula fragrans extract obtained by the method in preparing cosmetics with efficacy.

[0020] A cosmetic composition comprises the Inula fragrans extract or the Inula fragrans extract obtained by the method; in the cosmetic composition, the mass fraction of the Inula fragrans extract is greater than or equal to 0.5%.

[0021] In one embodiment, the cosmetic composition further comprises an auxiliary material; the auxiliary material comprises at least one of a moisturizer, a preservative, a stabilizer, a surfactant and water.

[0022] In one embodiment, the cosmetic composition is mainly composed of the following components in percentage by mass:

[0023] The composition includes 0.5% to 6% of Inula japonica extract, 0.02% to 0.08% of stabilizer, 1% to 3% of moisturizer, 0.5% to 1% of preservative, and the balance of water.

[0024] In one embodiment, the cosmetic composition is mainly composed of the following components in percentage by mass:

[0025] The composition includes 2% to 5% of Inula japonica extract, 0.03% to 0.05% of a stabilizer, 1.5% to 2.5% of a moisturizer, 0.5% to 1% of a preservative, and the balance of water.

[0026] In one embodiment, the stabilizer comprises disodium edetate.

[0027] In one embodiment, the humectant comprises glycerin.

[0028] In one embodiment, the preservative comprises p-hydroxyacetophenone, 1,2-hexanediol.

[0029] In one embodiment, the method for preparing the cosmetic composition comprises the following steps:

[0030] The mixture of stabilizer, moisturizer and water is subjected to heat treatment and then to cooling treatment to obtain a first raw material system; the first raw material system is mixed with Inula japonica extract to obtain a second raw material system; the second raw material system is mixed with a preservative to obtain a cosmetic composition.

[0031] In one embodiment, the heat treatment temperature is 85-90°C.

[0032] In one embodiment, the cooling treatment is performed to a temperature of 40-50°C.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The inula flower extract of the present invention has the effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up the apple cheeks, lifting the jawline, reducing the depth of pores, and improving skin elasticity.

[0035] (2) The preparation method of the Inula japonica extract of the present invention is simple, efficient and low-cost, and the obtained Inula japonica extract is rich in polysaccharides and polyphenols. Compared with the commonly used peptide raw materials for cosmetics with firming effects, the Inula japonica extract has the anti-wrinkle effect of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up apple muscles, lifting the jawline, reducing the depth of pores, and improving skin elasticity. The extraction process involved is relatively simple and low-cost.

[0036] (3) The cosmetic composition of the present invention has excellent anti-wrinkle effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up the apple cheeks, lifting the jawline, reducing the depth of pores, and improving skin elasticity. DETAILED DESCRIPTION

[0037] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. If no specific conditions are specified in the examples, the conditions are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be obtained commercially.

[0038] According to one aspect of the present invention, the present invention relates to an Inula flower extract, which comprises a water extract and / or an organic solvent extract of the Inula flower, wherein the volume fraction of the organic solvent is 0.01% to 100%.

[0039] The inula flower extract of the present invention is a water extract and / or a 0.01% to 100% organic solvent extract. The inula flower extract is rich in phenolic acid, flavonoids, polysaccharides, and sesquiterpenoid components, and has excellent anti-wrinkle effects on skin texture and crow's feet depth, such as reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up apple muscles, lifting the jawline, reducing the depth of pores, and improving skin elasticity.

[0040] In one embodiment, the organic solvent extract comprises an alcohol compound solution extract.

[0041] In one embodiment, the alcohol compound includes at least one of a monohydric alcohol and a polyhydric alcohol.

[0042] In one embodiment, the alcohol compound includes a medium polar alcohol compound and / or a low polar alcohol compound.

[0043] In one embodiment, the alcohol compound includes ethanol, propanol, propylene glycol, butanediol, pentanediol, octanediol, hexanediol, nonanediol, decanol, decanediol, 3-methoxybutanol, 3-hexenol, 4-terpineol, o-cymene-5-ol, aminopropylene glycol, aminomethyl propanol, aminomethyl propanediol, aminoethyl propanediol, phenylpropanol, benzyl alcohol, phenoxypropanediol, phenoxyethanol, phenoxyisopropanol, phenylethyl alcohol, denatured ethanol, butoxydiglycol, butoxyethanol, diethylene glycol, dimethyl Methoxychromanol, dithiooctanediol, dichlorobenzyl alcohol, diethoxydiglycol, dioleyl tocopheryl methylsilanol, olive oil alcohol, heptyl undecyl alcohol, cystine bis-PG-propyl silanetriol, silanetriol, decyl tetradecanol, methyl phenyl butanol, methyl benzyl alcohol, methoxymethyl butanol, methoxyisopropyl alcohol, cetyl alcohol, cetyl glycol, cetearyl alcohol, ceteareth-60 myristyl glycol, polydimethylsiloxane alcohol, polydimethylsiloxane alcohol panthenol, polyvinyl alcohol , kaempferol, kaempferol, cholesterol chloride, menthyl glycol, pinanediol, hydroxypropyl tetrahydropyrantriol, hydrogenated dimer linoleyl alcohol, hydrogenated tallow alcohol polyether-60 myristyl glycol, hydrogenated lanolin alcohol, sodium lactate methylsilanol, trifluoropropyl polydimethicone alcohol, triethylene glycol, trichlorobutanol, behenyl alcohol, cetyl stearyl alcohol, tetradecyl eicosanol, dipropylene glycol, diacetone alcohol, hydrolyzed silk PG-propyl methylsilanediol, hydrolyzed soy protein PG-propyl methylsilanediol, At least one of hydrolyzed collagen PG-propyl methylsilanediol, hydrolyzed collagen PG-propyl polydimethicone, tetrahydromagnanol, terpineol, tall oil sterol, amyl cinnamyl alcohol, citronellol, geraniol, caprylyl glycol, octyldecanol, octyldodecanol, lanosterol, coconut alcohol, ethyl linalool, acetylated lanolin alcohol, ethoxydiglycol, isobutyl methyltetrahydropyranol, isocetyl alcohol, stearyl alcohol, oleyl alcohol, lauryl alcohol, lauryl iminodipropylene glycol, phytol, phytantriol and palmitoleyl alcohol.

[0044] In one embodiment, the alcohol compound includes one or at least two of ethanol, ethanol, propanol, propylene glycol, butanediol, pentanediol, octanediol, hexanediol, nonanediol, decanol and decanediol.

[0045] In one embodiment, the method for preparing the Inula japonica extract comprises the following steps:

[0046] The first mixed system is obtained by soaking the inula flower in water or an organic solvent with a volume fraction of 0.01% to 100%. The first mixed system is subjected to heat reflux extraction to obtain a first mixed extraction system, and then solid-liquid separation is performed, and the liquid phase is collected to obtain the inula flower extract.

[0047] The preparation method of the Inula flower extract of the present invention is simple and easy, and is environmentally friendly. The obtained Inula flower extract contains polyphenols and polysaccharides, and has the anti-wrinkle effect of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up apple muscles, lifting the jawline, reducing the depth of pores, and improving skin elasticity.

[0048] In one embodiment, the dosage ratio of the Inula flower and water or the organic solvent with a volume fraction of 0.01% to 100% is 1g: (5 to 50) mL. In one embodiment, the dosage ratio of the Inula flower and the alcohol compound solution includes but is not limited to 1g: 5mL, 1g: 10mL, 1g: 20mL, 1g: 30mL, 1g: 40mL, 1g: 50mL, etc. By adopting a suitable material-liquid ratio, the active ingredients in the Inula flower are more easily dissolved to ensure the Inula flower extract has the anti-wrinkle effect of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping apple muscles, lifting the jawline, reducing pore depth, and improving skin elasticity.

[0049] The present invention soaks the Inula flower in advance, so that the Inula flower becomes soft and the cells swell, and the active ingredients are first dissolved in the Inula flower tissue. When a concentration difference occurs inside and outside the cell and a large osmotic pressure is generated, the active ingredients diffuse into the water outside the cell. In one embodiment, the soaking time is 20 to 40 minutes, such as 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, etc. The appropriate pre-soaking time is more conducive to the later extraction.

[0050] In one embodiment, the temperature of the hot reflux extraction is 78-80°C, such as 78°C, 78.5°C, 79°C, 795°C, 80°C, and the time of the hot reflux extraction is 50-80min, such as 50min, 55min, 60min, 65min, 70min, 75min or 80min, etc. The appropriate hot reflux temperature and time are conducive to the extraction of polyphenols and polysaccharides in Inula japonica, ensuring its efficacy.

[0051] According to another aspect of the present invention, the present invention also relates to the use of the Inula fragrans extract as described above or the Inula fragrans extract prepared by the method in preparing a cosmetic composition.

[0052] The inula flower extract of the present invention is used to prepare cosmetics, which can simultaneously have the excellent effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, reducing wrinkle depth, plumping apple muscles, lifting jaw lines, reducing pore depth, and improving skin elasticity.

[0053] According to another aspect of the present invention, the present invention also relates to a cosmetic composition, comprising the Inula fragrans extract or the Inula fragrans extract obtained by the method described; in the cosmetic composition, the mass fraction of the Inula fragrans extract is greater than or equal to 0.5%.

[0054] In one embodiment, the cosmetic composition further comprises an auxiliary material; the auxiliary material comprises at least one of a moisturizer, a stabilizer, a preservative, a stabilizer, a surfactant and water.

[0055] In one embodiment, the cosmetic composition is mainly composed of the following components in percentage by mass:

[0056] The composition includes 0.5% to 6% of Inula japonica extract, 0.02% to 0.08% of stabilizer, 1% to 3% of moisturizer, 0.5% to 1% of preservative, and the balance of water.

[0057] The present invention adopts appropriate amounts of Inula japonica flower extract, stabilizer, moisturizer and preservative to obtain a cosmetic composition with more excellent anti-wrinkle effect of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up apple muscles, lifting jawline, reducing pore depth and improving skin elasticity.

[0058] In one embodiment, the Inula japonica extract is 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5% or 6% by mass.

[0059] In one embodiment, the stabilizer is 0.02%, 0.025%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, etc. The present invention adopts a suitable amount of stabilizer, coordinated with other components, can reduce the irritation of cosmetics to the skin, enhance the anti-wrinkle, reduce the depth of pores, and improve the elasticity of the skin. In one embodiment, the stabilizer includes disodium ethylenediaminetetraacetic acid.

[0060] In one embodiment, the humectant is 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 3%, etc. The present invention adopts a suitable amount of humectant, and cooperates with other components to achieve better moisturizing effect, improve anti-wrinkle effect, reduce pore depth, and improve skin elasticity. In one embodiment, the humectant includes glycerol. Glycerol has a water absorption effect and can absorb water molecules in the air to keep the stratum corneum covered by it moist.

[0061] In one embodiment, the preservative is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc. The present invention has a better preservative effect by adding a suitable amount of preservatives and coordinating with other components. In one embodiment, the preservative includes at least one of phenoxyethanol, benzyl alcohol, phenylethyl alcohol, benzoic acid, sodium benzoate, p-hydroxyacetophenone, and 1,2-hexanediol.

[0062] In one embodiment, the cosmetic composition is mainly composed of the following components in percentage by mass:

[0063] 2% to 5% of Inula japonica extract, 0.03% to 0.05% of stabilizer, 1.5% to 2.5% of moisturizer, 0.5% to 1% of preservative, and the balance of water;

[0064] In one embodiment, the method for preparing the cosmetic composition comprises the following steps:

[0065] The mixture of stabilizer, moisturizer and water is subjected to heat treatment and then to cooling treatment to obtain a first raw material system; the first raw material system is mixed with Inula japonica extract to obtain a second raw material system; the second raw material system is mixed with a preservative to obtain a cosmetic composition.

[0066] The above-mentioned specific adding order of the present invention is more conducive to the full mixing and dissolution of each component, which is conducive to the better coordination of each component, so that the obtained cosmetic composition has better anti-wrinkle effect, pore depth reduction and skin elasticity improvement effect.

[0067] In one embodiment, the temperature of the heat treatment is 85-90°C, such as 85°C, 86°C, 87°C, 88°C, 89°C, 90°C, etc. By adopting a suitable temperature, the stabilizer and the humectant can be better dissolved and mixed. In one embodiment, the temperature of the cooling treatment is 40-50°C, such as 40°C, 42°C, 45°C, 48°C, 50°C, etc.

[0068] In summary, the present application provides an extract of Inula japonica, which has the effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, reducing the anti-wrinkle effect, plumping the apple muscles, lifting the jaw line, reducing the depth of pores, and improving skin elasticity. In verifying the above functions, the skin texture (smoothing skin) is evaluated by skin roughness (Ra and Rq), the anti-wrinkle effect of the raw material is evaluated by the depth of crow's feet, the plumping effect is evaluated by the volume of the apple muscles, and the skin firming effect is evaluated by the jaw angle, elasticity (R2), F4 (firmness), and pore depth.

[0069] The following is further explained in conjunction with specific embodiments and comparative examples.

[0070] Example 1

[0071] A method for preparing an Inula flower extract comprises the following steps:

[0072] (1) Soaking: Weigh a certain amount of Inula japonica, add 50% ethanol solution with a solid-liquid ratio of 1:20 (g / mL), and soak for 0.5 h;

[0073] (2) Extraction: 79°C reflux extraction for 1 h;

[0074] (3) Filtration: Filter to obtain a clear and transparent filtrate.

[0075] A cosmetic composition, comprising the following components in percentage by weight:

[0076] The present embodiment comprises 5% of the Inula japonica extract, 0.04% of disodium edetate, 2% of glycerol, 1% of a preservative, and the balance of water.

[0077] The preparation method of the cosmetic composition in this embodiment comprises the following steps:

[0078] (a) heating a mixture of disodium ethylenediaminetetraacetate, glycerol and water to 87° C., dissolving and stirring the mixture evenly, and then cooling the mixture to 45° C. to obtain a first raw material system;

[0079] (b) adding the Inula japonica extract to the first raw material system of step (a), stirring evenly, to obtain a second raw material system;

[0080] (c) adding the preservative into the second raw material system and stirring evenly;

[0081] (d) Samples that pass the inspection are discharged and marked as sample A.

[0082] Example 2

[0083] A method for preparing an Inula flower extract comprises the following steps:

[0084] (1) Soaking: Weigh a certain amount of Inula japonica, add 75% ethanol solution at a solid-liquid ratio of 1:20 (g / mL), and soak for 0.5 h;

[0085] (2) Extraction: 79°C reflux extraction for 1 h;

[0086] (3) Filtration: Filter to obtain a clear and transparent filtrate.

[0087] A cosmetic composition, comprising the following components in percentage by weight:

[0088] The present embodiment comprises 5% of the Inula japonica extract, 0.04% of disodium edetate, 2% of glycerol, 1% of a preservative, and the balance of water.

[0089] The preparation method of the cosmetic composition in this embodiment comprises the following steps:

[0090] (a) heating a mixture of disodium ethylenediaminetetraacetate, glycerol and water to 87° C., dissolving and stirring the mixture evenly, and then cooling the mixture to 45° C. to obtain a first raw material system;

[0091] (b) adding the Inula japonica extract to the first raw material system of step (a), stirring evenly, to obtain a second raw material system;

[0092] (c) adding the preservative into the second raw material system and stirring evenly;

[0093] (d) Samples that pass the inspection are discharged and marked as sample B.

[0094] Example 3

[0095] A method for preparing an Inula flower extract comprises the following steps:

[0096] (1) Soaking: Weigh a certain amount of Inula japonica, add 50% by volume butanediol solution at a solid-liquid ratio of 1:20 (g / mL), and soak for 0.5 h;

[0097] (2) Extraction: 79°C reflux extraction for 1 h;

[0098] (3) Filtration: Filter to obtain a clear and transparent filtrate.

[0099] A cosmetic composition, comprising the following components in percentage by weight:

[0100] The present embodiment comprises 5% of the Inula japonica extract, 0.04% of disodium edetate, 2% of glycerol, 1% of a preservative, and the balance of water.

[0101] The preparation method of the cosmetic composition in this embodiment comprises the following steps:

[0102] (a) heating a mixture of disodium ethylenediaminetetraacetate, glycerol and water to 87° C., dissolving and stirring the mixture evenly, and then cooling the mixture to 45° C. to obtain a first raw material system;

[0103] (b) adding the Inula japonica extract to the first raw material system of step (a), stirring evenly, to obtain a second raw material system;

[0104] (c) adding the preservative into the second raw material system and stirring evenly;

[0105] (d) Samples that pass the inspection are discharged and marked as C samples.

[0106] Example 4

[0107] A cosmetic composition, comprising the following components in percentage by weight:

[0108] Inula japonica extract 1%, disodium EDTA 0.04%, glycerin 2%, preservative 1%, and the balance is water.

[0109] The preparation method of the Inula japonica extract in this example is the same as that in Example 3.

[0110] The preparation method of the cosmetic composition in this example is the same as that in Example 3 except for the different proportions of the raw materials used. The product of this example is recorded as sample D.

[0111] Example 5

[0112] A cosmetic composition, comprising the following components in percentage by weight:

[0113] Inula japonica extract 0.5%, disodium edetate 0.04%, glycerin 2%, preservative 1%, and the balance water.

[0114] The preparation method of the Inula japonica extract in this example is the same as that in Example 3.

[0115] The preparation method of the cosmetic composition in this example is the same as that in Example 3 except for the different ratio of raw materials. The product of this example is recorded as Sample E.

[0116] Comparative Example 1

[0117] A cosmetic composition, comprising the following components in percentage by weight:

[0118] Rambutan leaf extract 5%, disodium EDTA 0.04%, glycerin 2%, preservative 1%, balance water.

[0119] The preparation method of the cosmetic composition in this comparative example is the same as that in Example 3 except that the raw materials and the usage ratio are different. The product of this comparative example is recorded as sample F.

[0120] Comparative Example 2

[0121] A composition comprising the following components in percentage by weight:

[0122] Disodium EDTA 0.04%, glycerin 2%, preservative 1%, the balance is water.

[0123] The preparation method of the composition in this comparative example comprises:

[0124] (a) heating a mixture of disodium ethylenediaminetetraacetate, glycerol and water to 87° C., dissolving and stirring the mixture evenly, and then cooling the mixture to 45° C. to obtain a first raw material system;

[0125] (b) adding the preservative to the first raw material system of step (a) and stirring evenly; the product of this comparative example is marked as sample G.

[0126] Comparative Example 3

[0127] A cosmetic composition, comprising the following components in percentage by weight:

[0128] Arnica flower extract 5%, disodium EDTA 0.04%, glycerin 2%, preservative 1%, the balance is water.

[0129] The preparation method of the cosmetic composition in this comparative example is the same as that in Example 3 except that the raw materials and the usage ratio are different. The product of this comparative example is recorded as sample H.

[0130] Comparative Example 4

[0131] A cosmetic composition, comprising the following components in percentage by weight:

[0132] Oatmeal firming protein 5%, disodium EDTA 0.04%, glycerin 2%, preservative 1%, the balance is water.

[0133] The preparation method of the cosmetic composition in this comparative example is the same as that in Example 3 except that the raw materials and the usage ratios are different. The product of this comparative example is recorded as Sample I.

[0134] Experimental example

[0135] Through human efficacy tests, it was verified that the extract of Inula japonica has the anti-wrinkle effect of reducing skin texture (smoothing skin), reducing the depth of crow's feet, plumping up the apple muscles, lifting the jawline, reducing the depth of pores, and improving skin elasticity.

[0136] 1. Subject recruitment

[0137] Recruit 120 volunteers.

[0138] Inclusion criteria:

[0139] ① People with sagging face;

[0140] ②Women aged 25-45 years old;

[0141] ③ Those who are willing to participate and can complete the required content according to the test requirements;

[0142] ④ Have not used products related to the tested efficacy in the past month, and have not received facial aesthetic treatments in the past 2 months;

[0143] ⑤ Those without severe systemic diseases, immunodeficiency or autoimmune diseases;

[0144] ⑥ Those without active allergic diseases;

[0145] ⑦ Those who have no history of allergy to skin care cosmetics;

[0146] ⑧ Those who have not used hormone drugs and immunosuppressants recently;

[0147] ⑨ Those who have not participated in other human experiments or clinical trials recently.

[0148] Exclusion criteria:

[0149] ①Those who do not meet the age requirement;

[0150] ② Those who have participated in the test on firming direction and its effect on skin condition within the last month;

[0151] ③People with skin allergic diseases;

[0152] ④ Those who are easily allergic to cosmetics;

[0153] ⑤ Patients who have recently used hormone drugs and have immunodeficiency or autoimmune diseases;

[0154] ⑥ Pregnant or lactating women

[0155] ⑦ Those with low degree of cooperation.

[0156] 2. Sample grouping

[0157] Group 1: A sample VS G sample.

[0158] Group 2: B samples vs. G samples.

[0159] Group 3: C sample VS G sample.

[0160] Group 4: D samples VS G samples.

[0161] Group 5: E sample VS G sample.

[0162] Group 6: C sample VS F sample.

[0163] Group 7: C sample VS H sample.

[0164] Group 8: C samples vs. I samples.

[0165] 3. Usage

[0166] Random half face use.

[0167] 4. Detection indicators

[0168] Skin roughness (Ra, Rq), crow's feet depth, apple muscle volume, jaw angle, pore depth, skin elasticity (R2 and F4);

[0169] In the skin roughness index, the roughness Ra represents: the arithmetic mean of the peak values ​​on the surface contour line of the skin in the tested local area. Rq represents: the square root of the average value of the peak values ​​on the contour line within the entire test length range. The smaller the value, the smoother the skin.

[0170] The larger the crow's feet depth and pore depth values ​​are, the shallower the crow's feet depth and pore depth are.

[0171] The volume of the apple muscles represents the fullness of the apple muscles. The larger the value, the fuller the apple muscles are.

[0172] The mandibular angle represents the angle between the horizontal line in the image and the mandible. An increase in the value indicates an increase in the mandibular angle.

[0173] In the skin elasticity test, the closer the elasticity R2 value is to 1, the better the skin elasticity; the smaller the F4 value is, the better the skin firmness is.

[0174] 5. Test sequence

[0175] Methods: Cleanse face (or sit quietly for 30 minutes) → take EVASKIN imaging pictures, VISIA-CR, and skin elasticity → use mask for 15 minutes, remove mask → cleanse face → collect EVASKIN imaging pictures, VISIA-CR, and skin elasticity.

[0176] 6. Test Results

[0177] (1) Skin texture improvement effect test The test site room temperature is (25±1)℃, relative humidity is (40±5)%, and the subjects should keep their bodies in a stable state before the test. After cleansing with the same cleansing product, the subjects should sit quietly in the test environment for 30 minutes before starting the test.

[0178] Before using the samples, the EVASKIN instrument was used to collect skin texture data of the test area and the control area.

[0179] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, the EVASKIN instrument was used to collect images of the left and right faces. All 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a mask matrix without Inula flower extract (control area); the 6th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competitor area); the 7th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an Arnica flower extract mask (competitor area); the 8th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competitor area). After 15 minutes, the mask was removed, and the face was cleansed with water. The EVASKIN instrument was used again to collect imaging data of the test area and the control area. Finally, the test areas on both sides of the cheeks were circled during treatment to obtain the skin texture roughness (Ra, Rq) data obtained during treatment, and t-test analysis was performed using SPSS statistical software.

[0180] The improvement effect of the Inula flower extract on skin texture was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 1.

[0181] Table 1 Effect of each group of extracts on improving skin texture

[0182]

[0183]

[0184] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0185] As can be seen from Table 1, when the 8 groups were compared within the group, the skin texture roughness Ra and Rq of sample A, sample B, sample C, sample D, sample F, and sample H were significantly reduced after use compared with before use (p < 0.05), indicating that the samples have the effect of reducing skin texture and smoothing the skin. There was no significant change before and after sample I, indicating that there was no effect of reducing skin texture and smoothing the skin; when compared between groups, when groups 1-4 used sample A, sample B, sample C, and sample D and compared with sample G, the skin texture roughness Ra and Rq were significantly lower than sample G (p < 0.05). In groups 6-8, the roughness Ra and Rq values ​​of sample C after use were significantly lower than those of sample F, sample H, and sample I (p < 0.05). Among them, in the difference of changes in the difference between sample C and competing products, the best ranking of the difference in skin roughness is sample C> sample F> sample H> sample I.

[0186] (2) Skin crow's feet depth improvement effect test The test site room temperature is (25±1)℃, relative humidity is (40±5)%, and the subjects should keep their bodies in a stable state before the test. After cleansing with the same cleansing product, the subjects should sit quietly in the test environment for 30 minutes before starting the test.

[0187] Before using the samples, the EVASKIN instrument was used to collect skin texture data of the test area and the control area.

[0188] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, the EVASKIN instrument was used to collect images of the left and right faces. All 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a mask matrix without Inula flower extract (control area); the 6th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competitor area); the 7th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an Arnica flower extract mask (competitor area); the 8th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competitor area). After 15 minutes, the mask was removed, and the face was cleansed with water and the EVASKIN instrument was used again to collect imaging data of the test area and the control area. Finally, the test areas on both sides of the cheeks were circled during treatment to obtain the crow's feet data of the treated skin, and t-test analysis was performed using SPSS statistical software.

[0189] The improvement effect of the Inula flower extract on the depth of crow's feet wrinkles was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 2.

[0190] Table 2 Changes in the depth of crow's feet in each group

[0191]

[0192]

[0193] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0194] As shown in Table 2, when the 8 groups were compared within the group, the depth of the crow's feet of the skin after using samples A, B, C, D, F, and H was significantly shallower than before (p < 0.05). When compared between groups, the depth of the crow's feet of groups 1-4 after using samples A, B, C, and D was significantly less than that of the G sample (p < 0.05). In group 6, the depth of the crow's feet of sample C was significantly shallower than that of sample F (p < 0.05). In group 7, the depth of the crow's feet of sample C was significantly shallower than that of sample H (p < 0.05). In group 8, the depth of the crow's feet of sample C was significantly shallower than that of sample I (p < 0.05). Among them, the best order of the difference in the change of the depth of crow's feet of sample C compared with the competing products is sample C>sample F>sample H>sample I.

[0195] (3) Test results for plumping up apple cheeks:

[0196] The room temperature of the test site is (25±1)℃, and the relative humidity is (40±5)%. The subjects should keep their bodies in a stable state before the test. After cleansing with the same cleansing product, sit quietly in the test environment for 30 minutes before starting the test.

[0197] Before using the samples, facial images of the test area and the control area were collected using the EVASKIN instrument.

[0198] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, the EVASKIN instrument was used to collect left and right facial images. Then, all 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula extract on half of their face (test area), and the other half applied a mask matrix without Inula extract (control area); the sixth group applied a mask containing Inula extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competing area); the seventh group applied a mask containing Inula extract on half of their face (test area), and the other half applied an Arnica extract mask (competing area); the eighth group applied a mask containing Inula extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competing area). After 15 minutes, the mask was removed, and the EVASKIN instrument was used again after cleansing with water to collect imaging data of the test area and the control area respectively. Finally, the apple muscle area was circled during processing, the apple muscle volume was processed, and t-test analysis was performed using SPSS statistical software. The effect of the Inula japonica extract on the plumping of the apple muscles was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 3.

[0199] Table 3 Changes in apple muscles of each group

[0200]

[0201] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0202] As shown in Table 3, when the 8 groups were compared within the group, the apple muscle volume of sample A, sample B, sample C, sample D, and sample H increased significantly after use compared with before use (p < 0.05). When compared between groups, the apple muscle volume of groups 1-4 after using sample A, sample B, sample C, and sample D was significantly higher than that of sample G (p < 0.05); in group 6, the apple muscle volume of sample C was significantly higher than that of sample F (p < 0.05). In group 7, the apple muscle volume of sample C was significantly higher than that of sample H (p < 0.05).

[0203] In Group 8, the apple muscle volume of sample C was significantly higher than that of sample I (p < 0.05). Among them, the order of the best apple muscle volume difference between sample C and its competitors was sample C > sample H > sample I > sample F.

[0204] (4) Jaw-line lifting test results:

[0205] The room temperature of the test site is (25±1)℃, and the relative humidity is (40±5)%. The subjects should keep their bodies in a stable state before the test. After cleansing with the same cleansing product, sit quietly in the test environment for 30 minutes before starting the test.

[0206] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, VISIA-CR imaging was used to collect frontal photos first. Then, all 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a mask matrix without Inula flower extract (control area); the sixth group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competitor area); the seventh group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an Arnica flower extract mask (competitor area); the eighth group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competitor area). After 15 minutes, the mask was removed, and the overlapping parts were collected again after cleansing with water. Frontal photos were collected, and the angles of the horizontal lines and the jaw line on the left and right sides were measured using PS software. SPSS statistical software was used for t-test analysis.

[0207] The effect of the extract of Inula japonica on jawline lifting was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 4.

[0208] Table 4 Changes in jaw line angles in each group

[0209]

[0210] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0211] As shown in Table 4, when the 8 groups were compared within the group, the jaw line angles of A, B, C, D, and I samples increased significantly after use compared with those before use (p < 0.05). When compared between groups, the jaw line angles of groups 1-4 after using A, B, C, and D samples were significantly higher than those of G samples (p < 0.05). In group 6, the jaw line angle of C sample was significantly higher than that of F sample (p < 0.05). In group 7, the jaw line angle of C sample was significantly higher than that of H sample (p < 0.05). In group 8, the jaw line angle of C sample was significantly higher than that of I sample (p < 0.05). Among them, the best ranking of the difference in the difference in jaw line angle between C sample and competing products is C sample > I sample > H sample > F sample.

[0212] (5) Skin pore depth improvement effect test The test site room temperature is (25±1)℃, relative humidity is (40±5)%, and the subjects should keep their bodies in a stable state before the test. After cleansing with the same cleansing product, the subjects should sit quietly in the test environment for 30 minutes before starting the test.

[0213] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, the EVASKIN instrument was used to collect images of the left and right faces. All 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a mask matrix without Inula flower extract (control area); the 6th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competitor area); the 7th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an Arnica flower extract mask (competitor area); the 8th group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competitor area). After 15 minutes, the mask was removed, and the face was cleansed with water. The EVASKIN instrument was used again to collect imaging data of the test area and the control area. Finally, the test area on both sides of the nose was circled for processing and the processed data were obtained. SPSS statistical software was used for t-test analysis.

[0214] The improvement effect of the Inula japonica extract on the depth of pores was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 5.

[0215] Table 5 Changes in pore depth in each group

[0216]

[0217]

[0218] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0219] As shown in Table 5, when the 8 groups were compared within the group, the pore depth of samples A, B, C, D and I after use was significantly shallower than before use (p < 0.05). When compared between groups, the pore depth of groups 1-4 after using samples A, B, C and D was significantly shallower than that of sample G; in group 6, the pore depth of sample C was significantly shallower than that of sample F. In group 7, the pore depth of sample C was significantly shallower than that of sample H. In group 8, the pore depth of sample C was significantly shallower than that of sample I. Among them, the best ranking of the difference in pore depth between sample C and competing products is sample C>sample I>sample H>sample F.

[0220] (6) Skin elasticity test results The room temperature of the test site was (25±1)℃ and the relative humidity was (40±5)%. The subjects were required to keep their bodies in a stable state before the test. After cleansing their faces with the same cleansing product, the subjects were required to sit quietly in the test environment for 30 minutes before the test began.

[0221] The test divided 120 volunteers into 8 groups. After cleansing and before using the samples, the skin elasticity tester Cutometer dualMPA580 was used to collect data on the left and right faces. All 8 groups of subjects underwent half-face experiments. The first 5 groups applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a mask matrix without Inula flower extract (control area); the sixth group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied a rambutan leaf extract mask (competitor area); the seventh group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an Arnica flower extract mask (competitor area); the eighth group applied a mask containing Inula flower extract on half of their face (test area), and the other half applied an oatmeal firming protein mask (competitor area). After 15 minutes, the mask was removed and the face was cleansed with water, and the elasticity data of the test area and the control area (R2 and F4) were collected again. Three points were collected each time, and finally the data of the three points were averaged to calculate the data on the left and right sides, and t-test analysis was performed using SPSS statistical software.

[0222] The improvement effect of the Inula flower extract on skin elasticity was evaluated by testing the changes in indicators before and after use and between the test area and the control area. The results are shown in Table 6.

[0223] Table 6 Changes in skin elasticity (R2, F4) in each group

[0224]

[0225] Note: *: compared with the same group, P < 0.05; #: compared with the other groups, P < 0.05.

[0226] As shown in Table 6, when the 8 groups were compared within the group, the skin elasticity of samples A, B, C, D, H, and I increased significantly after use (p < 0.05), and the skin firmness decreased significantly (p < 0.05). When compared between groups, in groups 1-4, when samples A, B, C, and D were used and compared with samples G, the skin elasticity was significantly higher than that of samples G (p < 0.05), and the firmness was significantly lower than that of samples G (p < 0.05); in group 6, the elasticity of sample C was significantly higher than that of sample F, and the firmness was significantly lower than that of sample F (p < 0.05). In group 7, the elasticity of sample C was significantly higher than that of sample H, and the firmness was significantly lower than that of sample H (p < 0.05). In group 8, the elasticity of sample C was significantly higher than that of sample I, and the firmness was significantly lower than that of sample I (p < 0.05). Among them, in the difference in changes between sample C and competing products, the best ranking of the difference in skin elasticity and firmness is sample C > sample I > sample H > sample F.

[0227] In summary, the cosmetic composition prepared from the Inula japonica extract of the present invention has excellent effects of reducing skin texture (smoothing skin), reducing the depth of crow's feet, and has anti-wrinkle effects, plumping up apple muscles, lifting jaw lines, reducing pore depth, and improving skin elasticity.

[0228] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An Inula flower extract, characterized in that: The extract comprises water extract and / or organic solvent extract of Inula japonica, and the volume fraction of the organic solvent is 0.01% to 100%.

2. The Inula japonica extract according to claim 1, characterized in that The organic solvent includes an alcohol compound solution; Preferably, the alcohol compound includes at least one of a monohydric alcohol and a polyhydric alcohol.

3. The method for preparing the Inula japonica extract according to claim 1 or 2, characterized in that: The following steps are involved: The first mixed system is obtained by soaking the inula flower in water or an organic solvent with a volume fraction of 0.01% to 100%. The first mixed system is subjected to heat reflux extraction to obtain a first mixed extraction system, and then solid-liquid separation is performed, and the liquid phase is collected to obtain the inula flower extract.

4. The method for preparing the Inula japonica extract according to claim 3, characterized in that: The dosage ratio of the Inula japonica flower to water or organic solvent is 1 g: (5-50) mL; Preferably, the soaking time is 20 to 40 minutes; Preferably, the temperature of the hot reflux extraction is 78-80° C., and the time of the hot reflux extraction is 50-80 min.

5. Use of the Inula fragrans extract according to claim 1 or 2 or the Inula fragrans extract obtained by the method according to claim 3 or 4 in the preparation of cosmetics.

6. A cosmetic composition, characterized in that The cosmetic composition comprises the Inula fragrans extract according to claim 1 or 2 or the Inula fragrans extract obtained by the method according to claim 3 or 4; in the cosmetic composition, the mass fraction of the Inula fragrans extract is greater than or equal to 0.5%.

7. The cosmetic composition according to claim 6, characterized in that The cosmetic composition further comprises auxiliary materials; The auxiliary materials include at least one of a moisturizer, a preservative, a stabilizer, a surfactant and water.

8. The cosmetic composition according to claim 6, characterized in that The cosmetic composition is mainly made of the following components in percentage by mass: The composition includes 0.5% to 6% of Inula japonica extract, 0.02% to 0.08% of stabilizer, 1% to 3% of moisturizer, 0.5% to 1% of preservative, and the balance of water.

9. The cosmetic composition according to claim 8, characterized in that The cosmetic composition is mainly made of the following components in percentage by mass: 2% to 5% of Inula japonica extract, 0.03% to 0.05% of stabilizer, 1.5% to 2.5% of moisturizer, 0.5% to 1% of preservative, and the balance of water; Preferably, the stabilizer comprises disodium ethylenediaminetetraacetate; Preferably, the humectant comprises glycerin; Preferably, the preservative comprises p-hydroxyacetophenone, 1,2-hexanediol.

10. The cosmetic composition according to claim 8 or 9, characterized in that: The preparation method of the cosmetic composition comprises the following steps: The mixture of stabilizer, moisturizer and water is subjected to heat treatment and then subjected to cooling treatment to obtain a first raw material system; the first raw material system is mixed with the Inula japonica extract to obtain a second raw material system; the second raw material system is mixed with a preservative to obtain a cosmetic composition; Preferably, the temperature of the heat treatment is 85-90°C; Preferably, the cooling treatment is performed to a temperature of 40-50°C.

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