The invention relates to a method for determining the residual quantity of 10 aminoglycoside antibiotics in eggs, and belongs to the technical field of trace veterinary drug residue detection. The determination method specifically comprises the following steps of: pre-treating a sample, preparing a standard stock solution, a mixed standard intermediate solution and a matrix matching working solution, performing determination by utilizing the matrix matching standard working solution and adopting hydrophilic interaction chromatography-triple quadrupole mass spectrometry, drawing a standard curve, and detecting the to-be-detected solution according to the same instrument parameters, thus obtaining mass chromatograms of the 10 aminoglycosides in the sample, and then carrying out qualitative and quantitative analysis. According to the invention, pollution of ion pair reagents to chromatography and mass spectrometry is avoided, according to the detection method provided by the invention, pollution of heptafluorobutyric acid, sodium heptanesulfonate and other ions to a chromatographic column and a mass spectrometry detector is avoided; the pretreatment operation is simple, the purification effect is good, the sensitivity is higher, the detection limit and the quantitation limit are greatly reduced, and the limit requirement of the national standard on aminoglycoside in eggs is completely met.