34results about How to "Large peak capacity" patented technology

The invention relates to a high-efficiency high-flux method of connecting two-dimension array liquid chromatogram and mass spectrum. After separated by the first-dimension chromatogram, the analyzed sample enters the second dimension for further high-efficiency separation; the first dimension enters the second-dimension chromatogram from the flow interface through a single chromatographic column; the mobile phase of the second dimension is hit by a chromatogram pump and then is divided by a dividing device through the interface and then hit into the second-dimension chromatographic column. The second-dimension chromatographic column adopts the array mode, and each chromatographic column in the array is synchronously separated with the same chromatographic conditions, the outlet of each chromatographic column of the second dimension is connected with one channel of the multi-channel detector to conduct synchronous high-sensitivity detection. The invention has the advantages of large system peak capacity, high separating efficiency, sensitive detection, simple online classification, large commercial prospect and extensive analyzing object.

The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.

The invention relates to a multidimensional chromatography combination separation device, which comprises a pressurized capillary electrochromatography unit. The pressurized capillary electrochromatography unit comprises a mobile phase driving device, an injection valve, a capillary chromatographic column and a buffer solution bottle which are connected mutually and sequentially, a high-voltage power supply connected in parallel with the capillary chromatographic column, and a detector connected on the capillary chromatographic column. The separation device also comprises a capillary ion-exchange chromatography unit. The capillary ion-exchange chromatography unit comprises a mobile phase driving device, an injection valve and a capillary ion-exchange chromatography column which are connected mutually and sequentially. The capillary ion-exchange chromatography column is connected with an injection port of the injection valve of the pressurized capillary electrochromatography unit. The multidimensional chromatography combination separation device has the advantages of high separation efficiency, large peak capacity, small sample dosage, low raw material cost and low pollution, has more excellent peak capacity and separation capacity than the conventional one-dimensional chromatography, pressurized capillary electrochromatography, two-dimensional liquid chromatography and the like, and is more suitable for analysis of the complex sample.

The invention relates to a chromatographic system with double or multiple series columns. The chromatographic system comprises a liquid delivery system, a separation system, a detection system and a collecting system which are sequentially connected with one another. The liquid delivery system comprises a liquid delivery pump and a pressure measuring apparatus, the detection system comprises a UV (ultraviolet) detector, a PH (potential of hydrogen) detector and an electric conductivity detector, the collecting system comprises a collecting valve and a collector, the separation system comprises two or more than two separation units which are serially connected with one another, each separation unit comprises switching valves and a chromatographic column, the chromatographic column and the switching valves of each separation unit are connected with one another, the various separation units are serially connected with one another by pipelines, and the chromatographic columns of the various separation units are connected with one another or are disconnected from one another under the control of the switching valves of the various separation units. The chromatographic system with the double or multiple series columns has the advantages that to-be-analyzed components in a complicated sample can be roughly and precisely separated from one another by the chromatographic system, and the chromatographic system is high in separation capacity, selectivity, peak capacity, resolution and applicability.

The invention relates to the capillary electrophoresis method in stereo multiple dimensions and multiple modes as well as its special equipment. Switching voltage makes the mobile phase be switched between different flow paths. The sample components are separated into at least two capillary columns by using different separation modes of the capillary electrophoresis through flow paths in forms of series connection, parallel connection or their combination. The special equipment is mainly composed of at least two caillary columns, at least one connecting piece and high voltage source. The connecting piece transports the samples between columns. The container containing flow path is installed on the capillary columns or the free end of the vacant capillary.

The invention provides an enrichment-thermal desorption-chromatography separating unit. The enrichment-thermal desorption-chromatography separating unit is composed of a rapid enrichment thermal desorption device, a sample introduction device, and a rapid separating device; the rapid enrichment thermal desorption device is used for realizing low temperature enrichment of target components in gas samples using an adsorption tube at the action of low temperature of carbon dioxide gas, and realizing rapid heating thermal desorption via a heating wire, wherein an adsorbent is arranged in the adsorption tube, and the adsorption tube is enwound by the heating wire; after enrichment and desorption, the target components are delivered into the rapid separating device via the sample introduction device; a low temperature carbon dioxide gas cooling chromatographic column is adopted in the rapid separating device, relatively low chromatographic column initial temperature and relatively high heating rate are achieved via combination of the low temperature carbon dioxide gas cooling chromatographic column with a rapid heating module, so that high separation effect, extremely high analysis speed, and high peak capacity are achieved. The enrichment-thermal desorption-chromatography separating unit is capable of realizing on-line enrichment, rapid desorption, and super rapid gas chromatography of trace components in gas samples.

The invention belongs to the technical field of proteomics, and in particular relates to a two-dimensional conventional column array type chromatographic separation system for removing high-abundance proteins and a separation method applying the system. According to the invention, only one multi-element liquid phase pump, one ten-way valve and two six-way valves are needed for connection and on-line two-dimensional switching; and the automation degree and the integration degree of an instrument are improved. Fractions obtained by performing first-dimensional IE separation on a protein sample are directly and sequentially switched through the ten-way valve and transferred to a column head of a multi-channel second-dimensional conventional reversed-phase column for enrichment; the reversed-phase column directly performs on-line desalting and gradient dilution on the protein sample; by two-dimensional conventional chromatographic separation, the high-abundance proteins can be precisely positioned on a two-dimensional chromatogram; and therefore, the accurate removal aim is fulfilled. By the method, large-volume sample injection, concentration desalting, array type separation and positioning and removal of the high-abundance proteins can be finished; and the two-dimensional conventional column array type chromatographic separation system is very suitable for positioning, removal and high-flux analysis for the high-abundance proteins required by research on proteomics.

The invention relates to an online volatile organic compound detector and method. The online volatile organic compound detector comprises an online preconcentration system, a full two-dimensional gaschromatography device and a controller, wherein the online preconcentration system is connected with the full two-dimensional gas chromatography device, and the two are both connected with the controller; the controller controls the online preconcentration system to capture a to-be-detected sample gas in an online mode, performs corresponding preconcentration and heating analysis processing and send the gas to the full two-dimensional gas chromatography device, completion signals are sent to the controller, and the controller controls the full two-dimensional gas chromatography device to startaccording to the completion signals; and the full two-dimensional gas chromatography device separates the to-be-detected sample gas after detection processing according to preset separation detectionand analysis conditions, and a mass chromatogram is obtained. The full two-dimensional gas chromatography device has the characteristics of high resolution, high sensitivity, fast response, and the like. The obtained mass chromatogram has high reliability. Quantitative and qualitative analysis are carried out according to the mass chromatogram and a preset standard mass chromatogram, and the components of a complex volatile organic compound can be analyzed highly reliably.

The invention relates to an analytical method of nicotinic acid content in food, which adopts a pressurized capillary electrochromatography for analysis and comprises the steps as follows: the food to be analyzed is used for preparing sample solution; the prepared sample solution is directly injected into the pressurized capillary electrochromatography for carrying out the analysis; and an obtained chromatographic peak figure is compared with a standard chromatographic peak figure of the nicotinic acid, and the normalization method is used for calculating the percentage content of the nicotinic acid in the sample, etc. The mobile phase used by the analytical method of the invention is relatively simple, the using amount of the sample is less, the peak capacity is great, the analytical process is stable, and the method has good precision and separating effect.

The invention provides a method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry. The method comprises the steps of adding an internal standardsample into a tobacco sample, adding a solvent, extracting, centrifuging and filtering to obtain a sample solution; and then, measuring the sample solution by adopting a two-dimensional liquid chromatography-mass spectrometry combination method, qualitatively determining the sucrose ester component and the internal standard sample in the sample solution by mass spectrometry, quantitatively determining the internal standard sample in the sample solution through a standard curve method, and determining the relative content of the sucrose ester component with the structure similar to that of theinternal standard sample determined quantitatively in the sample solution. The invention further provides a multi-dimensional liquid chromatography-mass spectrometry combined analysis system and a using method thereof. The method for analyzing the sucrose ester in the tobacco by the multi-dimensional liquid chromatography-mass spectrometry can effectively remove interfering substances which influence the quantification of the sucrose ester in the tobacco, has the characteristics of being high in accuracy, good in reproducibility and low in detection limit, the analysis can be automatically accomplished through an instrument, and the method is suitable for the batch detection of the tobacco sample.

The invention discloses a method for rapidly screening banned additive essence and flavor in single cream. The result shows that in the concentrations of essence and flavor and related chemicals in single cream are in corresponding ranges, a correlation coefficient (r2) is greater than 0.99 and a good linear relationship is formed. The method has a determination limit of 0.01-5.35 micrograms / kilogram, a detection capacity of 0.01-8.32 micrograms / kilogram, a recovery rate of 81%-115% and a relative standard deviation of 0.2%-6.4%. The method utilizes a known compound and unknown compound screening and analysis method, has online DSPE-full two-dimensional gas chromatography-time-of-flight mass spectrometry characteristics of fast analysis speed, high resolution, large peak capacity, high accuracy and high precision and realizes instrumental system screening of essence and flavor in single cream through the online optimization of DSPE and chromatography and mass spectrometry condition parameters.

The invention discloses a construction method and application of a fingerprint of sweet dream oral liquid. The method comprises the following steps: preparing a test solution, preparing a reference solution, optimizing and determining chromatographic conditions, measuring the fingerprint and processing the obtained fingerprint by using fingerprint software. Chromatographic columns with different stationary phases are connected through a six-way valve; gradient elution is performed by the same mobile phase; and various characteristic index components are well separated. Compared with an existing established fingerprint method, the method achieves that characteristic components of the sweet dream oral liquid can be separated and detected as many as possible, obtains more fingerprint characteristic peaks, and more comprehensively reflects component information of the oral liquid. The method further has the advantages of being easy and convenient to operate, high in precision, good in stability and reproducibility and the like, and the quality of the sweet dream oral liquid can be evaluated more comprehensively and scientifically, so that the quality and the curative effect of the product are guaranteed, and reference is provided for perfection of the quality standard of the product.

The invention discloses a method for identifying metabolites of phoxim in different tissue of freshwater fishes. A soaking method is adopted for drug delivery, and an ultra-high performance liquid chromatography tandem quadrupole flight time high-resolution mass spectrum (UPLC-QTOFMS) is used for qualitatively detecting the metabolites of phoxim in different tissue. The method comprises the following steps that 1, different tissue is collected after the freshwater fishes are soaked in a phoxim solution; 2, samples are extracted, wherein an extracting agent is obtained by mixing acetonitrile and ethyl acetate in the volume ratio of 5:1, and a purifying agent is prepared by mixing anhydrous magnesium sulfate and octadecyl silane bonded silica gel in the mass ratio 10:1; 3, UPLC-QTOFMS quantitative analysis is carried out, wherein the metabolites of the tissue samples are judged by being compared with phoxim, O,O,O',O',-tetraethyl dithiopyrophosphate and O,O-diethyl thiophosphate in the aspects of retention time and ion mass.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a characterization method of a secondary metabolite chromatogram and a mass spectrum fingerprintspectrum of callicarpa nudiflora genuine medicinal materials. According to the method, the UPLC is adopted to establish the full-component fingerprint spectrum of a callicarpa nudiflora genuine medicinal material, and the UPLC and DAD / QTOF-MS / MS combined technology is adopted to carry out on-line rapid component qualitative analysis and compound identification on the full-component fingerprint spectrum of the callicarpa nudiflora genuine medicinal material and the optimal chemical substance phenotype of the callicarpa nudiflora genuine medicinal material in the specific natural environment isformed. The callicarpa nudiflora genuine medicinal material full-component fingerprint spectrum method has the beneficial effects that the operation is simple, the peak capacity is large, the precision, the reproducibility and the stability are good, the chromatographic peak separation degree is good, the analysis time is short, and the analysis efficiency and the sensitivity are high; the formedoptimal phenotype of chemical substances of the callicarpa nudiflora genuine medicinal material in a specific natural environment solve the problems of disordered application of callicarpa nudiflorabase sources, incapability of representing genuine medicinal materials and the like in the market are solved.

The invention provides an extraction method of an anticancer active monomer in Curcuma zedoaria. The method includes: taking Curcuma zedoaria tuber as the raw material, grinding the Curcuma zedoaria tuber into powder, then conducting ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The invention provides a new compound never reported before, the process has the characteristics of simplicity, low cost, large preparation capacity and good repeatability, and the obtained monomer compound has high purity, good biological activity and anticancer drug development potential.

The invention relates to a chromatographic system with double or multiple series columns. The chromatographic system comprises a liquid delivery system, a separation system, a detection system and a collecting system which are sequentially connected with one another. The liquid delivery system comprises a liquid delivery pump and a pressure measuring apparatus, the detection system comprises a UV (ultraviolet) detector, a PH (potential of hydrogen) detector and an electric conductivity detector, the collecting system comprises a collecting valve and a collector, the separation system comprises two or more than two separation units which are serially connected with one another, each separation unit comprises switching valves and a chromatographic column, the chromatographic column and the switching valves of each separation unit are connected with one another, the various separation units are serially connected with one another by pipelines, and the chromatographic columns of the various separation units are connected with one another or are disconnected from one another under the control of the switching valves of the various separation units. The chromatographic system with the double or multiple series columns has the advantages that to-be-analyzed components in a complicated sample can be roughly and precisely separated from one another by the chromatographic system, and the chromatographic system is high in separation capacity, selectivity, peak capacity, resolution and applicability.

The invention discloses a device for improving the peak capacity of capillary electrophoresis through a capillary electrophoresis two-step speed difference mode. The device comprises a negative pole liquid storage tank (1), a positive pole liquid storage tank (2), a sample tank (4) and a capillary tube (5) and further comprises a first zero-pole liquid storage tank (3) and a second zero-pole liquid storage tank (13); the capillary tube (5) is arranged to penetrate through the first zero-pole liquid storage tank (3) and the second zero-pole liquid storage tank (13), one end of the capillary tube (5) is movably connected with the positive pole liquid storage tank (2) or the sample tank (4), the other end of the capillary tube (5) is movably connected with the negative pole liquid storage tank (1) or the sample tank (4), and a gap which can conduct electricity and cannot conduct mass transfer is formed in the portion, in each zero-pole liquid storage tank, of the capillary tube. By means of the device for improving the peak capacity of the capillary electrophoresis, the requirement for the self separation capacity of the capillary tube is reduced, even though partial components in a sample cannot reach the baseline separation, the baseline separation can be finally achieved by means of the method, and therefore the whole peak capacity of the capillary electrophoresis is improved. In addition, a low content component signal is not lost when the peak capacity is improved through the device.

The invention discloses a method for separating or analyzing a phenolic acid compound from a burdock. The method comprises a joint use process of an efficient liquid chromatograph and an ion mobilityspectrometer, wherein the ion mobility spectrometer is an electrospray-ion mobility spectrometer. Through the method, phenolic acid compound ingredients in the burdock can be quickly separated or analyzed, and an application basis is provided for new burdock medicine development.

The invention discloses a hydrophilic silica gel chromatographic packing for separating a water soluble polymer and a protein and a preparation method of the hydrophilic silica gel chromatographic packing. The preparation method comprises the following steps: (A), activating silica gels to prepare activated silica gels; (B), carrying out epoxy modification on the activated silica gels so as to prepare epoxy-based modified silica gels; (C), introducing a hydrophilic polymer to the epoxy-based modified silica gels so as to prepare hydrophilic polymer modified silica gels; and (D), hydrolyzing the hydrophilic polymer modified silica gels so as to prepare the hydrophilic silica gel chromatographic packing for separating the water soluble polymer and the protein. The hydrophilic silica gel chromatographic packing disclosed by the invention has the advantages of larger pore volume, lower hydrophobicity, higher number of theoretical plates, and larger peak capacity.

The invention relates to a method for evaluating cigarette flavor compensation technology. In the method, the distribution rule of the chemical composition of a top flavor in a cigarette product can be visually represented by utilizing the particular two-dimensional distribution of a two-dimensional gas chromatogram (comprising instruments and methods, such as the two-dimensional gas chromatogram, a two-dimensional liquid chromatogram and the like capable of displaying two-dimensional spectrograms), and further, a cigarette flavor compensation effect is evaluated. Through comparing the chemical compositions of the top flavor, unflavored cut tobacco and flavored tobacco and combining with sensory evaluation, the distribution and the action of the chemical composition of the top flavor in a tobacco blend are analyzed so as to know whether a certain top flavor has a head flavor, a body flavor and a basic flavor or not or is harmonious with the self flavor of the cigarette or not, thereby, a reference is provided for a low-tar cigarette flavor compensation technology. The method has the advantages of more convenience and rapidness, visibility, accuracy and the like.

The invention provides a new compound, which has a molecular formula of C15H20O3, a molecular weight of 248, and a Chinese name of 8-hydroxy-Germa-1, 7-diene-5one-8, 12-lactone. The preparation method includes: taking curcuma zedoaria tuber as the raw material, conducting grinding into powder, then performing ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The new compound is proved to have anti-liver cancer and anti-lung cancer biological activity and can be used for preparation of related drugs.

The invention provides an identification method used for ballpoint pen ink, which adopts a pressurized capillary electro-chromatography instrument for the identification, comprising the steps as follows: a sample solution is prepared by the ballpoint pen ink to be identified; the sampling analysis of the prepared sample solution is carried out by the pressurized capillary electro-chromatography instrument; furthermore, the obtained chromatographic peak graph is compared with the known chromatographic peak graph of the ballpoint pen ink, thus identifying the sort and type of the ballpoint pen ink to be identified, etc.; wherein, the sampling quantity is 700nl and the analysis time is 40min. The identification method of the invention uses relatively simple flow phase, and has little sample quantity, large peak capacity, stable analysis process, good preciseness and extremely good separation effect.

The invention belongs to the technical field of proteomics, and in particular relates to a two-dimensional conventional column array type chromatographic separation system for removing high-abundance proteins and a separation method applying the system. According to the invention, only one multi-element liquid phase pump, one ten-way valve and two six-way valves are needed for connection and on-line two-dimensional switching; and the automation degree and the integration degree of an instrument are improved. Fractions obtained by performing first-dimensional IE separation on a protein sample are directly and sequentially switched through the ten-way valve and transferred to a column head of a multi-channel second-dimensional conventional reversed-phase column for enrichment; the reversed-phase column directly performs on-line desalting and gradient dilution on the protein sample; by two-dimensional conventional chromatographic separation, the high-abundance proteins can be precisely positioned on a two-dimensional chromatogram; and therefore, the accurate removal aim is fulfilled. By the method, large-volume sample injection, concentration desalting, array type separation and positioning and removal of the high-abundance proteins can be finished; and the two-dimensional conventional column array type chromatographic separation system is very suitable for positioning, removal and high-flux analysis for the high-abundance proteins required by research on proteomics.

The invention discloses a method for separating or analyzing phenolic compounds from burdock, which comprises the process of combining high performance liquid chromatography-ion mobility spectrometer, and the ion mobility spectrometer is electrospray-ion mobility spectrometer . The invention can rapidly separate or analyze the phenolic acid compounds in the burdock, and provides an application basis for the development of new burdock drugs.

The invention relates to the capillary electrophoresis method in stereo multiple dimensions and multiple modes as well as its special equipment. Switching voltage makes the mobile phase be switched between different flow paths. The sample components are separated into at least two capillary columns by using different separation modes of the capillary electrophoresis through flow paths in forms of series connection, parallel connection or their combination. The special equipment is mainly composed of at least two caillary columns, at least one connecting piece and high voltage source. The connecting piece transports the samples between columns. The container containing flow path is installed on the capillary columns or the free end of the vacant capillary.

The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.

The invention relates to the technical field of traditional Chinese medicine drug analysis, in particular to a method for characterizing secondary metabolite chromatography and mass spectrometry fingerprints of a medicinal material of Radix Nutria. The present invention adopts UPLC to establish the fingerprint spectrum of the whole components of the medicinal material of Nasturtica chinensis, and adopts the combined technology of UPLC and DAD / QTOF-MS / MS to perform the online rapid component qualitative analysis of the fingerprint spectrum of the whole components of the medicinal material of Rhododendron chinensis and its compounds. Identify and form the best phenotype of the chemical substances in the specific natural environment of the natural medicinal material. The beneficial effects of the invention are as follows: the fingerprint spectrum method of the whole components of the medicinal material of Radix japonica is simple to operate, the peak capacity is large, and the precision, reproducibility and stability are all good, the chromatographic peak separation is good, the analysis time is short, and the analysis time is short. The efficiency and sensitivity are high; the best phenotype of the chemical substances in the specific natural environment of the natural medicinal material is formed, which solves the problems of confusion in the application of the base source of the naked flower and the inability to characterize the authentic medicinal material in the market.