New compound and preparation method thereof
A compound, the technology of n-hexane, applied in organic chemistry, drug combination, antineoplastic drugs, etc., can solve the problem of difficult to ensure the effectiveness of clinical medication, safety and sustainability, lack of material basis for drug effect and drug effect research, zedoary volatile oil Complicated components and other issues, to achieve quality and controllable drug use, broaden the source of raw materials, good separation effect
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Embodiment 1
[0041] Use curcuma as raw material, grind it into powder, weigh 4kg, dissolve it in 25L of 98% ethanol solution, and carry out reflux extraction, and finally obtain the filtrate by rotary evaporation to dryness, and then dissolve it in n-hexane-ethanol with a volume fraction of n-hexane of 70%. In a binary mixed solvent, prepare an extract with a concentration of 100 mg / mL, pass through a 0.45 μm microporous membrane, and prepare by one-dimensional liquid chromatography.
[0042] One-dimensional liquid chromatography adopts Silica150×250mm, 10μm, The mobile phase adopts the mixture of organic phases, wherein the organic phase A phase is n-hexane, B phase is ethanol, gradient elution mode: B phase concentration 0-50% gradient elution for 30 minutes. Adopt the ultraviolet detector 210nm to select the absorption wavelength, the preparation temperature is room temperature, the injection volume is 100mL / needle, the mobile phase flow rate is 600mL / min, collect the component of 15.5...
Embodiment 2
[0046] The purpose component that embodiment 1 obtains utilizes analytical chromatographic column to carry out purity test, as image 3 As shown, the purity reaches 99%, and it is a monomeric compound. The chromatographic column selects the normal phase silica gel chromatographic column Silica4.6×250mm, 10μm,
[0047] The target monomer compound was subjected to NMR analysis (BrukerA.GAVIII600PLUS, Hanmeng Biotechnology (Tianjin) Co., Ltd.), and the NMR spectrum was as follows: Figure 4 , 5 As shown, the NMR data of the monomer compound obtained according to 1HNMR and 13CNMR spectrograms are shown in Table 1.
[0048] Table 1: 1HNMR (600MHz) and 13CNMR (600MHz) (CDCl 3 ,TMS,ppm)
[0049] Carbon number
δC
δH(JinHz)
1
134.05
4.90(d,10.4)
2
27.30
1.95(m)2.26(m)
3
36.13
1.71(m)2.05(m)
4
47.91
2,47(m)
5
209.27
6
40.26
3.30(d,15.2),3.58(d15.2)
7
154.45
...
Embodiment 3
[0053] Embodiment 3: Human liver cancer cell Hep--G2 experiment
[0054] Combine the eight-well plate (E-PlateL8) produced by Eisen Bio (Hangzhou) Co., Ltd. with the iCELLigence real-time label-free cell function analyzer, add 150ulF-12K medium to each well, and place at 37°C, 5% CO 2 In the incubator, adjust the background data to between 40-130. The compound sample (EZ-8-3) isolated in Example 1 was completely dissolved to 50 mg / ml with dimethyl sulfoxide, and then diluted into a 5 mg / ml working solution with F-12K medium, and the cultured Human liver cancer cells Hep-G2 were digested with trypsin and diluted to 4×10 4 / ml live cell suspension, inoculated in eight-well plate, 345ul per hole, added 5ul of working solution to make the final concentration 50μg / ml, and added 5ul of 1‰ dimethyl sulfoxide F- 12K medium, placed at 37°C, 5% CO 2 culture medium for 72 hours. The results showed that the target monomer compound 8-hydroxy-jima-1,7-dien-5-one-8,12-lactone significant...
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