2 results about "Normal phase" patented technology

The application belongs to the technical field of detection and analysis, and discloses a normal phase detection method for imine reaction control. The method is detected by normal phase liquid chromatography, and the detection conditions include: a polysaccharide derivative normal phase coated type chiral chromatographic column is used, and a mixed solution of n-hexane, ethanol, ethylenediamine and trifluoroacetic acid is used as the mobile phase, wherein the volume ratio of n-hexane, ethanol, ethylenediamine and trifluoroacetic acid is (75-85):(15-25):(0.05-0.15):(0.05-0.15). The method can realize effective separation, detect the content of 1,2,3,4,10,14B-hexahydrodibenzo[C,F]pyrazino[1,2-A]azepine in the reaction solution, and has the advantages of simplicity, accuracy, rapidness and reliability.

The invention provides a high performance liquid chromatography analysis method of paricalcitol related substances. By changing a mobile phase system, changing a reverse phase elution system into a normal phase elution system and adjusting the column temperature, the separation degree of a main peak and a plurality of related impurities is improved, related substances in paricalcitol can be accurately detected, and the separation effect is outstanding. The analysis method disclosed by the invention is stable in baseline, good in stability, good in specificity and high in sensitivity, and has very important significance for comprehensively, accurately and objectively evaluating the product quality.