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Method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology

A technology of gas chromatography mass spectrometry and aroma components, which is applied in the direction of material separation, material analysis, measuring devices, etc.

Inactive Publication Date: 2010-03-03
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
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Problems solved by technology

However, no related research has been carried out in the analysis of tobacco components, especially in the analysis of tobacco flavor components.

Method used

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  • Method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology
  • Method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology
  • Method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology

Examples

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Embodiment

[0029] Examples Tobacco powder

[0030] 1. At the same time, one end of the distillation extraction device is connected to a 1000mL flat-bottomed flask containing 25g of tobacco powder, 350mL of water and 90g of sodium chloride, and is heated by an electric furnace with controllable voltage. At the same time, the other end of the distillation extraction device was connected to a 100 mL flask filled with 45 mL of dichloromethane, and this end was heated on a water bath, and the temperature of the water bath was 60°C. After the extraction was completed, 9 g of anhydrous sodium sulfate was added and dried overnight. The extract was then concentrated to 1 mL.

[0031] 2. The concentrated sample is separated by normal phase liquid chromatography: select amino analysis column (250mm×2.0mmi.d.), n-hexane-dichloromethane-acetonitrile (volume ratio is 90:6.6:3.4) as the mobile phase, inject the sample The volume is 5 μL, and the mobile phase flow rate is 0.2mL / min. Under this chromat...

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Abstract

A method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography / mass spectrometry technology comprises the following steps: 1. taking a given weight of tobacco products and extracting the volatile and semi-volatile flavor components from the tobacco by simultaneously adopting distillation and extraction; 2. concentrating the extracted samples with a concentrator; 3. carrying out group component separation on the concentrated extracted samples by normal-phase liquid chromatography and collecting distillates by different time sections; and 4. concentrating the collected distillates by the concentrator and respectively entering a gas chromatography-mass spectrometer (GC-MS) for detailed analysis. Compared with theprior art, the method of the invention can effectively extract the volatile and semi-volatile flavor components from the tobacco, carry out group component separation on the extract liquor by normal-phase liquid chromatography, then carry out accurate GC-MS qualitative and quantitative analysis on each group component according to different boiling points, not only has good selectivity and high separation efficiency but also ensures more accurate qualitative and quantitative analysis of each component.

Description

technical field [0001] The invention relates to a method for separating and analyzing tobacco components, in particular to a method for separating and analyzing volatile and semi-volatile aroma components in tobacco by using liquid chromatography-gas chromatography-mass spectrometry. Then the concentrated sample is separated by normal phase liquid chromatography, and the fractions are collected, and finally the collected fractions are subjected to gas-mass analysis, which realizes efficient and highly selective separation and analysis of tobacco aroma components. Background technique [0002] The chemical components in tobacco and smoke are very complex. According to relevant literature reports, more than 5,000 compounds have been found in tobacco and cigarette smoke (Dube, M.F. and Green, C.R.36 th TCRC, 1982). Among these compounds, the flavor components that have an important impact on cigarette smoking mainly come from volatile and semi-volatile substances in tobacco. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 刘绍锋白军超谢复炜刘惠民夏巧玲张晓兵赵晓东郭吉兆赵阁
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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