77 results about "Volatile flavor" patented technology

The invention relates to a rhodotorula mucilaginosa strain and a yellow wine co-fermentation process thereof, and belongs to the technical field of fermentation engineering and strains. The invention provides the rhodotorula mucilaginosa with the fermentation characteristics of low temperature resistance, acid resistance, high yield of beta-glucosidase and aroma production, sequential inoculation is adopted, the rhodotorula mucilaginosa H04 is firstly inoculated for fermentation for two days, then the saccharomyces cerevisiae is inoculated, and the yellow wine co-fermentation is carried out by an equal-proportion 20 DEG C low-temperature fermentation process. The yellow rice wine meeting national standard requirements of yellow rice wine can be obtained after 20 days of fermentation, and the volatile flavor type, content, taste and aroma sensory quality of the yellow rice wine after fermentation are improved.

The present application relates to the technical field of vinegar brewing, and discloses a method for brewing Guizhou sun-dried vinegar by using high-temperature Daqu to ferment wine mash, which relies on the unique microbial ecological advantages of the Chishui River basin, and comprises the following steps: firstly, pretreating glutinous rice, then adding the pretreated glutinous rice into local sauce-flavor high-temperature Daqu to prepare wine mash through alcohol fermentation; secondly, mixing the wine mash with bran and chaff at a ratio of 3L:1kg:0.125kg, and inoculating the mixture with traditional sun-dried vinegar seed koji; thirdly, completing vinegar koji fermentation by following the solid-state layered fermentation process of vinegar koji; and finally, obtaining finished sun-dried vinegar through three sets of circulating vinegar spraying, pasteurization, daily sunning and night airing aging. The present application optimizes the flavor basis of wine mash from the source, increases the total content of esters in vinegar koji by 22.1% and the total content of acids by 28.1%, introduces three kinds of volatile flavor substances unique to high-temperature Daqu, shortens the sunning aging period from 1-3 years to 6-8 months, and forms a differentiated flavor with both sauce-flavor heritage and sun-dried vinegar original flavor, thereby providing a new path for the quality upgrading and characteristic development of Guizhou sun-dried vinegar industry.

The application provides application of bacitracin or a composition of the bacitracin and oregano essential oil in improving meat quality of ruminants, and relates to the technical field of animal feed. The bacitracin is used as an active ingredient for preparing feed, and the addition amount of the bacitracin in the feed is 1.0-2.4 g / kg. The active ingredient also comprises oregano essential oil, and the mass ratio of the bacitracin to the oregano essential oil is 3.5-4.0:1. The bacitracin can be used for improving meat quality of ruminants for the first time, and when the bacitracin is used in combination with the oregano essential oil, synergistic effects can be produced in meat color, tenderness, fatty acid composition and formation of volatile flavor substances, muscle quality of different parts is improved, meat quality is effectively improved, the comprehensive commodity value of ruminant meat is greatly improved, and the bacitracin has a good popularization prospect.

The present application relates to a kind of natural osmanthus incense type micro powder lining paper perfuming method, natural osmanthus is crushed to obtain osmanthus powder, the volatile flavoring components of natural osmanthus and osmanthus powder are determined, and the loss of flavoring components is compared, the olfactory threshold of each substance is determined using three-point selection method, the key aroma-producing components with higher contribution degree of osmanthus powder loss are determined, and the transfer rate of each flavoring substance is calculated by HS-SPME-GC-MS, the transfer rate of flavoring substance obtained and the difference before and after each flavoring component loss are calculated to obtain the flavoring agent that each gram of osmanthus powder needs to supplement the key aroma-producing component composition, after dilution with anhydrous ethanol, it is sprayed on the surface of osmanthus powder;After osmanthus powder after flavoring is mixed evenly with coating liquid and is applied to the surface of cigarette lining paper, the coated lining paper is applied to cigarette production.The present application utilizes the load aroma function of osmanthus micro powder to better impart natural osmanthus incense to cigarette lining paper, and the aroma of ordinary imitation flavoring oil is more persistent.

The utility model discloses a device for extracting fragrance components in a curing barn, which comprises a shell, a rotating drum is arranged in the shell, a cover plate is fixedly connected above the inner wall of the rotating drum, the top of the cover plate is fixedly connected with a rotating rod which is rotatably connected with the inner wall of the shell through a sealing bearing, and the rotating rod is fixedly connected with the inner wall of the shell through the sealing bearing. A light phase flow guide disc fixedly connected with the upper portion of the outer wall of the shell is attached to the upper portion of the outer wall of the rotary drum, and a light phase outlet is communicated with the upper portion of one side of the shell. The utility model relates to the technical field of tobaccos, in particular to a device for extracting aroma components in a flue-curing barn, which works together in a centrifugal extraction mode and a flue-curing barn condensate water collection mode. Volatile flavor components in the curing barn are changed into condensate water through the flue-cured tobacco curing barn condensate water collecting device, the condensate water directly enters the shell to be extracted so as to collect the flavor components in the curing barn, the whole device is sealed in the extraction stage, and loss of an extracting agent and the flavor components is reduced.

The invention relates to the technical field of food fermentation and food flavor improvement. The invention relates to a fermentation product, in particular to a flavor improving method of a daylily fermentation product and application thereof. The flavor improving method comprises the following steps: S1, carrying out fixation treatment on cleaned day lily, and pulping to obtain day lily slurry; s2, performing low-temperature plasma treatment on the day lily slurry obtained in the step S1; s3, inoculating pre-activated saccharomycetes and lactobacillus plantarum into the day lily slurry subjected to low-temperature plasma treatment, and fermenting in a fermentation environment to obtain a day lily fermentation product; wherein the generation of various volatile flavor substances in the day lily fermentation process is promoted through the synergistic regulation effect of the low-temperature plasma treatment and the microbial fermentation process, so that the aroma component types of the fermentation product are richer; meanwhile, excessive accumulation of low-molecular alcohol substances, such as ethanol, isobutanol and isoamyl alcohol, which are easy to generate pungent smell is inhibited, so that the overall flavor coordination and quality of the daylily fermented product are improved.

The present application relates to food preservation technology field, propose a kind of additive influence analysis method on goose meat storage quality, steps include: after pretreatment, goose meat is placed in pickling liquid containing additive and pickled;After pickling, halogenation, vacuum packaging and secondary boiling water sterilization are carried out, and stored in cold storage condition;During storage, total number of colonies, pH value, TVBN value, TBARS value, color difference, texture characteristics, sensory evaluation score and volatile flavor substances are detected periodically.The present application evaluates the influence of different additives on the storage quality of marinated goose through multi-dimensional index system, and the results show that: composite natural preservative can significantly inhibit microbial growth and fat oxidation, extend the shelf life to more than 35 days, and improve texture and color;Fresh spices and hot reaction goose meat essence give marinated goose rich layered flavor and prominent spicy characteristics respectively.The present application provides experimental basis for the application of natural composite additives in marinated goose preservation and flavor control, and can guide industrial production.

The invention discloses a method for rapidly identifying production places and years of dried orange peel based on a GC-IMS technology and a specific volatile flavor substance proportion model, and belongs to the field of food science and analytical chemistry. According to the present invention, Guangdong Xinhui Citrus reticulata 'Citrus reticulata' dried orange peel, Sichuan Citrus reticulata 'Dahongpao' dried orange peel, and Zhejiang Taizhou Citrus reticulata 'Unshiu' dried orange peel are adopted as research objects, and the difference of the volatile flavor substances of the dried orange peel of different producing areas and aging years is compared and analyzed; through a difference comparison diagram, a fingerprint spectrum and a PCA diagram and by constructing a dual model of the proportion of specific volatile substances and the total amount of large-class substances, dried orange peel samples of different producing areas and aging time can be visually distinguished. The method is based on the GC-IMS technology, the pretreatment is simple, the analysis is rapid, the sensitivity is high, the explicitly quantified scale model is combined, the standardization degree of the method is high, and the method is easy to popularize and apply to market supervision and enterprise quality control.

The present application relates to a kind of extraction method and extraction concentration equipment of spearmint flavor, the method uses liquid nitrogen brittle treatment, low-temperature ultrasonic extraction, two-stage ultrafiltration purification, then liquid-liquid extraction by ethyl acetate, the organic phase is concentrated in vacuum;Concentrated solvent vapor generated is introduced into microbubble in separation tank two, and the water phase residual flavor component, concentrated volatile flavor component and ethyl acetate are recovered synchronously.The equipment includes separation tank one, separation tank two, concentration tank and vacuum pump, micro gas plate, temperature control module and the like, and the extraction agent and flavor recovery rate are improved by pressure boosting and low-temperature control.The present application can efficiently recover flavor component, improve the total yield of aroma, reduce solvent loss and flavor loss.

The invention belongs to the technical field of food processing, and particularly relates to a method for improving the flavor of instant fish head soup, the fish head soup is prepared by ultrasonic-assisted boiling, and the flavor of the perch fish head soup is improved in combination with fermented bacillus tropicalis SCSMX-8. The taste components and volatile flavor components of the perch head soup and the fermented fish head soup are measured by methods such as sensory evaluation, peptidomics, solid-phase microextraction / gas chromatography-mass spectrometry and the like, and the influence of a bacillus tropicalis fermentation method on the flavor characteristics of the perch head soup is explored. According to the invention, a technical basis is provided for development and industrial production of the lateolabrax japonicus head soup and the pretreated product thereof, and lateolabrax japonicus byproducts which are not fully utilized are converted into economically feasible and environmentally sustainable food.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

The application discloses a Pediococcus acidilactici for improving color and flavor of low-salt solid-state soy sauce and belongs to the technical field of bioengineering. The application obtains a Pediococcus acidilactici WT1 which can grow well under higher temperature and certain salinity by being separated and screened from soy sauce mash. The P.acidilactici WT1 is strengthened to carry out low-salt solid-state soy sauce fermentation, so that the amino acid nitrogen of the soy sauce is increased by 9.43%, the contents of succinic acid and tartaric acid in the soy sauce are respectively increased by 71.87% and 5.43 times, the contents of umami and sweet amino acids are respectively increased by 35.16% and 13.44%, the total amount of volatile flavor substances is increased by 19.95%, and the color rate is increased by 21.36%. By strengthening the P.acidilactici WT1, the contents of main flavor substances, i.e., guaiacol and phenol in the soy sauce are respectively increased by 35.49% and 50.64%, and the contents of benzyl alcohol and isoamyl alcohol are respectively increased by 16.83% and 21.86%. Therefore, strengthening the P.acidilactici WT1 in the low-salt solid-state soy sauce fermentation process has important significance for improving the quality and flavor of the low-salt solid-state soy sauce.

This invention provides a type of yeast called *Saccharomyces clavatum*, characterized in that the *Saccharomyces clavatum* is: *Saccharomyces clavatum* (… Saccharomycopsis fibuligera AMCC 32248, preservation number CCTCC NO: M 20251908. Enzyme activity phenotypic screening, carbon source utilization analysis, and quantitative enzyme activity detection showed that this strain possesses highly efficient β-glucosidase activity; further analysis of key genes related to β-glucosidase... BGL1 Specific functional sequences were extracted and identified. Enzyme production conditions were optimized using response surface methodology, resulting in a significant increase in enzyme activity compared to before optimization. In a simulated fermentation system, this strain significantly promoted the production of specific acids, esters, aldehydes, alcohols, and other volatile flavor compounds. Its enzyme production characteristics and flavor regulation capabilities provide a new technical solution for addressing issues such as low hydrolysis efficiency of glycosides and insufficient flavor release in fermentation systems, showing potential applications in the food, brewing, feed, and pharmaceutical industries.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method and application of high-activity bacterium enzyme complex liquid for fermenting fermented soybean cakes. The activity of protease, alpha-amylase and lipase in the fermented soya bean cake prepared by the method is improved; soluble protein and polypeptide are improved compared with naturally-fermented fermented soya bean cakes; the contents of total acid, reducing sugar, amino acid nitrogen, free amino acid and fatty acid are higher than those of naturally-fermented black bean cakes, but the content of NaCl is remarkably reduced; and the content of volatile flavor substances is obviously increased compared with that of naturally fermented black bean cakes. The fermented soya bean cakes are fermented by the method, so that the bitter taste, astringent taste and salty taste of the fermented soya bean cakes can be reduced, and the delicate flavor and the taste richness of the fermented soya bean cakes are improved. The method can improve the quality and edible safety of the fermented bean products in multiple aspects, and provides high-quality strains and technical support for industrial safe production of the fermented bean products.

The invention provides precise coffee baking and flavor preserving equipment, which belongs to the technical field of coffee bean processing and comprises a box shell component, a hot air component, a turnover shaft body component, a baking cylinder component, a rotary driving component, an outer cover component and a feeding and air returning switching component. According to the equipment, coffee beans are evenly heated through rotation of the baking barrel, and accurate temperature control is achieved through a hot air assembly and a temperature sensor; the innovation is that an air return mode is set, the preheating stage is a non-air return mode and used for adding air flow for circulating hot air into the top box body, and the baking stage is switched to a closed-loop air return mode, so that volatile flavor substances are circulated and enriched in the system, and flavor loss can be effectively reduced; after baking is finished, cold air can be switched for rapid cooling, and automatic forward-tilting discharging is achieved by turning over the shaft body. According to the equipment, automatic control from feeding, preheating, sealed return air baking to cooling and discharging is achieved, and the coffee flavor retention effect is remarkably improved while the baking curve is precisely regulated and controlled.

The invention provides saccharomycopsis fibuligera, which is characterized in that the saccharomycopsis fibuligera is saccharomycopsis fibuligera AMCC 32248, and the preservation number of the saccharomycopsis fibuligera is CCTCC (China Center for Type Culture Collection) NO: M 20251908. Enzyme activity phenotype screening, carbon source utilization analysis and enzyme activity quantitative detection show that the strain has efficient beta-glucosidase activity; furthermore, the key gene BGL1 of the beta-glucosidase is called, and a specific functional sequence is excavated. The enzyme production condition is optimized through a response surface method, and the enzyme activity is remarkably improved compared with that before optimization. In a simulated fermentation system, the strain can remarkably promote generation of volatile flavor substances such as specific acids, esters, aldehydes and alcohols, and a new technical scheme is provided for solving the problems of low hydrolysis efficiency of glycoside substances, insufficient flavor release and the like in the fermentation system due to the enzyme production characteristic and flavor regulation and control capability of the strain. The method has application potential in the fields of food, brewing, feed, medicine and the like.

The invention discloses a citrus essential oil volatile flavor component analysis method, and relates to the technical field of food science, the analysis steps are as follows: S1, sample preparation: selecting fresh citrus fruits, separating peels, extracting citrus essential oil by a cold pressing method, placing the essential oil sample in a headspace bottle, sealing, and standing at normal temperature for 15 min; s2, solid-phase micro-extraction enrichment: using an extraction head, inserting the extraction head into a headspace bottle after aging treatment, pushing out the extraction head, adsorbing at the headspace position for 35 minutes, and taking back a fiber head. According to the method, the SPME conditions are optimized, the extraction head is selected, adsorption is controlled at 40 DEG C for 35 min, the enrichment efficiency of the low-content volatile components in the citrus essential oil is remarkably improved, a scientific basis is provided for essential oil quality grading and product formula optimization, the method is easy and convenient to operate, no organic solvent is needed, reagent consumption is low, the detection period is short, and the method is suitable for industrial production. The method is suitable for batch detection and industrial application of citrus essential oil.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

The application discloses a lactobacillus buchneri with blood sugar reducing activity and application of the lactobacillus buchneri in fermented milk, the strain is lactobacillus buchneri PB6, which is preserved in the Guangdong Microbial Culture Collection Center on May 19, 2025, and the preservation number is GDMCC No: 66352, the strain has blood sugar reducing activity, safety and probiotic property, has good tolerance to gastrointestinal environment, has excellent self-aggregation capacity, antioxidant capacity, and can inhibit the growth of common pathogenic bacteria, and is helpful to maintaining the balance of intestinal flora. The application of the lactobacillus buchneri PB6 in fermented milk can improve the sensory quality and rheological properties of the fermented milk, and has blood sugar reducing activity, rich organic acids and volatile flavor substances, and provides a basis for the application of the strain in functional food.

The invention discloses a fresh meat pretreatment method for preparing dry salted meat, and belongs to the technical field of food processing. According to the method, through a four-section type collaborative process of fresh meat primary treatment, low-temperature plasma activation, probiotic compounding, plant extract soaking, light enzymolysis and low-temperature standing, low-temperature plasma of mixed gas of oxygen and nitrogen is adopted for forming pores in the surface of fresh meat; and performing vacuum pulsating soaking with a composite treatment liquid containing probiotics, a rosemary extract, tea polyphenols, fructo-oligosaccharide and trehalose. Chemical additives such as phosphate and nitrite are not needed, the sterilization rate of fresh meat can be greatly increased, the water loss rate after dry pickling is greatly reduced, the content of free amino acids and volatile flavor substances is increased, meanwhile, special parameters are designed for pig meat, cattle meat and mutton to ensure uniform quality, the total time consumption of pretreatment is shortened, and the cost is reduced. The technical bottlenecks of insufficient sterilization, poor water binding capacity, flavor loss and dependence on chemical additives in traditional pretreatment are effectively broken through.

The invention provides a method for detecting flavor substances in camellia oleosa seed oil, which realizes efficient enrichment and accurate qualitative and quantitative detection of volatile flavor substances in camellia oleosa seed oil by optimizing sample pretreatment, HS-SPME extraction conditions and gas chromatography-mass spectrometry (GC-MS) detection parameters. And a characteristic flavor substance is screened in combination with a relative odor activity value (ROAV). The method comprises the following specific steps: pretreatment of a camellia oleosa seed oil sample, enrichment of flavor substances through headspace solid-phase microextraction, GC-MS separation and detection, data processing and flavor substance identification, and screening of characteristic flavor substances. The method is easy and convenient to operate, high in enrichment efficiency and high in detection sensitivity, flavor substances such as aldehydes, alcohols and esters in the camellia oleosa seed oil can be comprehensively captured, characteristic components (such as nonanal, alpha-cedrene and capraldehyde) contributing key aroma are accurately screened out, and a scientific basis is provided for camellia oleosa seed oil quality evaluation, variety breeding and processing technology optimization.

The invention discloses a method for screening high-flavor-quality chicken individuals by detecting specific SNP locus genotypes, a related kit and application thereof, and belongs to the technical field of molecular breeding. The SNP molecular marker combination comprises four specific sites, namely, chr3: 100011635 (G / A), chr3: 100011636 (T / C), chr3: 100011645 (C / T) and chr3: 100011656 (A / T), which are positioned in a chicken KCNS3 gene region. The invention also provides a specific primer pair for detecting the molecular marker combination and a kit containing the primer. By detecting the genotypes of chicken individuals to be detected at the sites, excellent individuals with high volatile flavor substance content can be quickly and accurately screened out. According to the method, high-quality chicken breeds can be accurately screened, the flavor quality of meat products is improved, the problems that high-quality chicken breeding is long in period, low in efficiency and poor in selection accuracy are solved, an efficient and reliable molecular technical means is provided for early breeding of chicken flavor characters, and the breeding process is remarkably accelerated.

The invention discloses a salty chicken flavor enhancer as well as a preparation method and application thereof, and belongs to the technical field of food processing. The salty and fragrant chicken flavor enhancer is prepared from tea powder, salt, kaempferia galanga powder, gardenia jasminoides ellis powder and edible oil. According to the method, sensory analysis, an electronic tongue, an electronic nose, gas chromatography-mass spectrometry (GC-MS) and flavor amino acid and free fatty acid index analysis are combined, the improvement effect of different tea leaves and spices on the flavor quality of the salted chicken is analyzed, and a detection result shows that the effect of a black tea group is optimal. Therefore, the invention provides the salty and fragrant chicken flavor enhancer containing the black tea powder, and the salty and fragrant chicken flavor enhancer can improve the contents of alcohol volatile flavor substances, aldehyde volatile flavor substances, acid volatile flavor substances, ketone volatile flavor substances, flavor free amino acids and fatty acids in chicken. The invention provides a theoretical basis for flavor optimization and quality regulation and control of the salty chicken.

The application discloses a method for detecting the mildew of soybean meal based on volatile flavor substances, and relates to the technical field of food / feed safety detection, and comprises a soybean meal sample pretreatment step and the following steps: directional enrichment-purification, double-trigger reaction, double-mode detection and result output; in the application, the binary limitation of only judging whether mildew exists or not is broken through temperature-responsive double-ligand affinity microspheres, the aspergillus flavus, aspergillus niger and penicillium are accurately distinguished through an enzyme-antibody double-trigger recognition unit to fill the risk assessment fault, the double-mode detection of graphite powder-carbon black composite conductive hydrogel and indicator takes into account the precision and industrialization cost, the database matching is combined to realize the pollution link traceability and storage environment optimization, and finally, the principle contradiction between the specificity of mold metabolism and the broad spectrum of detection technology is resolved, and the integrated precision identification-risk assessment-targeted prevention and control of soybean meal mildew is achieved.

The invention provides a rapid pre-discrimination method for outputting sensory values according to flavor characteristics of cow milk. The rapid pre-discrimination method comprises the following steps: performing program optimization on a raw milk sample by adopting HS-SPME-GC-MS to determine volatile matters; heating the raw milk and then carrying out sensory evaluation; measuring by an electronic nose to obtain a sensor response value; establishing a sensory value prediction model based on volatile flavor factor data or fusion of volatile flavor factors and sensor signal data, and evaluating a model effect; and introducing a new sample, and pre-judging the flavor attribute according to the two established models. According to the method, a multi-data fusion method is creatively adopted, that is, the accuracy is higher by combining the temperament data and the electronic nose data and establishing the model with the sensory values, the model accuracy can be improved by increasing the sample number in a gradient mode, and the sensory values are more comprehensively and accurately output. The reason for establishing the model for the two types of data is that the sensory value information can be predicted more accurately according to which data source, and the subjective error and time cost of sensory evaluation personnel are effectively reduced.

The invention discloses lactobacillus brevis and an application of the lactobacillus brevis in solid-state fermentation of bunge pricklyash leaves, the lactobacillus brevis is lactobacillus brevis RS3, the lactobacillus brevis is registered and preserved in the China Center for Type Culture Collection on October 20, 2025, and the preservation number is CCTCC NO: M20252257. The lactobacillus brevis is lactobacillus brevis RS3, the lactobacillus brevis RS3 is lactobacillus brevis RS3, the lactobacillus brevis RS3 is lactobacillus brevis RS3, the lactobacillus brevis RS3 is lactobacillus brevis RS3, and the lactobacillus brevis RS3 is lactobacillus brevis RS3 and is preserved in the China Center for Type Culture Collection on October 20, 2025. The 16s rDNA (ribosomal Deoxyribose Nucleic Acid) sequence of the lactobacillus brevis is SEQ ID NO: 1. The lactobacillus brevis RS3 disclosed by the invention is applied to solid-state fermentation of the bunge pricklyash leaves, so that the nutritional value of a bunge pricklyash leaf fermentation product can be improved, the oxidation resistance can be enhanced, the content of anti-nutritional factors such as phytic acid and tannin in the bunge pricklyash leaves can be reduced, and the safety and digestibility of the bunge pricklyash leaves in feed can be improved; meanwhile, organic acid generated through fermentation can prolong the shelf life, generated volatile flavor substances improve the original pungent smell of the bunge pricklyash leaves, the palatability of the bunge pricklyash leaves in the feed is improved, and animal ingestion is facilitated.

The invention relates to the technical field of microorganisms, in particular to a zygosaccharomyces rouxii ZB445 and Weissella fusiformis ZF629 composite zymophyte agent and application of the zygosaccharomyces rouxii ZB445 and Weissella fusiformis ZF629 composite zymophyte agent. The compound fermentation inoculant provided by the invention has high stress resistance and excellent capability of producing alcohol and ester volatile flavor substances, is used for fermenting the thick broad-bean sauce, and can obviously increase the content of various key volatile compounds in the thick broad-bean sauce, including the total content of flavor substances such as alcohol and ester, so that the yield of the thick broad-bean sauce is increased. The content of key flavor compounds, especially phenethyl alcohol, 3-methylthio propyl alcohol, eugenol, ethyl caprylate and the like, is reduced, and meanwhile, excellent flavor amino acid accumulation capacity is also shown. The composite fermentation inoculant is used for fermenting the thick broad-bean sauce, so that the mellow fragrance and the fruit ester fragrance of the thick broad-bean sauce can be remarkably improved, the fresh, sweet and mellow feeling is enhanced, the overall fragrance and flavor of the thick broad-bean sauce are improved, the flavor and taste of the thick broad-bean sauce are richer, more complex, more harmonious and layered, and the overall flavor and quality of the thick broad-bean sauce are remarkably improved.

The invention discloses a method for detecting mildewing of soybean meal based on volatile flavor substances, and relates to the technical field of food / feed safety detection.The method comprises a soybean meal sample pretreatment step and further comprises the following steps of directional enrichment-purification, double-trigger reaction, double-mode detection and result output; according to the invention, the binary limitation that whether mildewing or not can only be judged is broken through the temperature response type double-ligand affinity microspheres, and aspergillus flavus, aspergillus niger and penicillium are accurately distinguished through the enzymatic-immune double-trigger recognition unit so as to fill the risk assessment fault; through dual-mode detection of graphite powder-carbon black composite conductive hydrogel and an indicator, the precision and the industrialization cost are both considered, and database matching is combined to realize pollution link traceability and storage environment optimization, so that the principle contradiction between the mildew metabolism specificity and the detection technology broad spectrum is finally solved. The integration of accurate recognition of soybean meal mildewing, risk assessment and targeted prevention and control is achieved.

The invention provides a flavor maintaining method and system for seasoned sea skirt vegetables, and relates to the technical field of marine food manufacturing, the technical scheme is characterized in that first parameter information is obtained, the content difference between volatile flavor substances and non-volatile flavor components is judged, the first parameter information comprises the content of characteristic flavor substances of raw materials before operation, and the first parameter information comprises the content of the characteristic flavor substances of the raw materials before operation; the characteristic flavor substances comprise volatile flavor substances and non-volatile umami components; when the content of the volatile flavor substance is greater than the content of the non-volatile flavor component, generating a fragrance enhancing scheme; and when the content of the volatile flavor substance is less than that of the non-volatile umami component, generating a freshness enhancing scheme. The method and the system for maintaining the flavor of the seasoned sea skirt vegetable, provided by the invention, have the advantages that accurate adaptation of the seasoning direction is realized according to the delicate flavor components of the sea skirt vegetable, the flavor advantages of the sea skirt vegetable are exerted, and the flavor quality is highlighted.