863 results about "Aspergillus niger" patented technology

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The invention discloses a compound microbial agent for enhancing production of beta-phenethyl alcohol in a white spirit brewing process as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of microorganisms. The compound microbial agent is prepared from aspergillus niger, pichia membranaefaciens, Kazakstan monospora, saccharomyces cerevisiae and sporospora delbrueckii; in the compound microbial agent, the final concentration OD600 of the aspergillus niger, the final concentration OD600 of the pichia membranaefaciens, the final concentration OD600 of the Kazakstein monospora, the final concentration OD600 of the saccharomyces cerevisiae and the final concentration OD600 of the sporospora delbrueckii are 0.8-1.5, 0.8-1.5, 0.5-1, 0.8-1.5 and 0.5-1 respectively. After the compound microbial agent provided by the invention is applied to a solid-state fermentation system of Luzhou-flavor liquor, the yield of the aroma substance beta-phenethyl alcohol can be obviously increased by adjusting the eukaryotic microbial community structure of the fermentation system; the method can effectively solve the problem that the stability of the method for producing beta-phenethyl alcohol in the process of intensifying Baijiu fermentation by a single strain is poor.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly discloses an acid protease mutant and application thereof. According to the invention, genome DNA from Aspergillus niger is taken as a basis, a parent acid protease gene sequence is obtained through PCR amplification, random mutation is carried out through error-prone PCR, and an acid protease mutant with improved enzyme activity and a recombinant strain thereof are obtained through screening. After modification, under the optimal condition, the enzyme activity of the acid protease mutant is 251.64 U / mL, the enzyme activity is improved by 23.43% compared with that of parent acid protease (203.64 U / mL), meanwhile, the acid protease mutant is not prone to inactivation in the reaction process, has higher stability, can tolerate strong acid and high salt environments, shows a higher conversion rate in enzymolysis of collagen, and can be used for preparing the acid protease mutant. The leather softening agent has a good application prospect in leather softening.

The invention relates to the technical field of grease chemistry, in particular to a preparation method and application of diglyceride oil capable of preventing and treating fatty liver. The preparation method comprises the following steps: (1) mixing raw material oil, water, glycerol and a lipase preparation for catalytic reaction to obtain a product 1; the raw material oil comprises 0.1-8 parts of sea buckthorn seed oil, 0.1-8 parts of acer truncatum seed oil, 60-90 parts of low erucic acid rapeseed oil, 15-35 parts of corn oil and 10-25 parts of peanut oil; the lipase preparation is aspergillus niger lipase and rhizopus niger lipase; (2) performing five-stage molecular distillation on the product 1 to obtain a product 2; and (3) decoloring and deodorizing the product 2 to obtain the product. The diglyceride oil prepared by the invention has excellent effects of preventing and treating fatty liver.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention discloses a livestock composite biological feed prepared from probiotics and a method thereof, and relates to the technical field of livestock feeds. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus niger BNCC147472, enterococcus faecalis BNCC186075, yarrowia lipolytica BNCC193899, nutrient substances, mineral trace elements, vitamins, amino acids and prebiotics. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus The compound biological feed fermented by adopting various probiotics can improve the antioxidant level of the broiler chicken, improve the health condition of intestinal tracts, increase the villus height and the villus-implicit ratio of the jejunum of the broiler chicken and reduce the occurrence of intestinal inflammation, so that the growth performance of the broiler chicken is promoted, and the immune performance of the body is improved.

The invention belongs to the field of microorganisms, and particularly relates to aspergillus niger, a microbial agent, solid-state fermentation liquor as well as a preparation method and application of the solid-state fermentation liquor. The Aspergillus niger is Aspergillus niger AGM01, the Aspergillus niger is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Aspergillus niger is CCTCC NO: M2024768. The aspergillus niger AGM01 provided by the invention has relatively high enzyme activity of beta-glucosidase. The aspergillus niger AGM01 is subjected to fermentation culture through the solid-state fermentation culture medium containing the small molecule inducer to obtain the aspergillus niger solid-state fermentation liquid, and the enzyme activity of beta-glucosidase in the aspergillus niger solid-state fermentation liquid can be improved.

The invention discloses fermented shrimp paste and a bacterial strain used by the same. The strain for fermenting the shrimp paste is at least one of aspergillus, aspergillus niger, mucor and / or rhizopus oryzae. The fermented shrimp paste has excellent indexes such as amino nitrogen, volatile basic nitrogen, flavor, color and the like.

The invention relates to bacillus velezensis and application thereof in prevention and treatment of wheat leaf blight and inhibition of plant pathogenic fungi, and belongs to the field of biological prevention and treatment. The bacillus velezensis HXYT7 is separated and screened from tobacco leaves growing in a tobacco plantation (the longitude is 113.73 and the latitude is 33.91) in Xiangchang County in elm city, Henan province, has a remarkable inhibition effect on mophila, fusarium moniliforme, fusarium graminearum, rhizoctonia cerealis, aspergillus flavus and aspergillus niger, can damage the hypha structure of the mophila, and can be used for preparing the bacillus velezensis HXYT7. The bacillus subtilis can inhibit germination of spores of the helminthosporium, has an obvious prevention and treatment effect on the wheat leaf blight caused by the helminthosporium, and provides a new microbial resource for prevention and treatment of the wheat leaf blight.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention relates to the field of microorganisms, and discloses a microbial composite inoculant and a method for degrading lignocellulose. The compound microbial agent comprises phanerochaete chrysosporium, aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum. The phanerochaete chrysosporium is a white-rot fungus with strong performance, and can secrete a plurality of lignocelluloses at the same time. Aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum are mainly used for secreting high-activity cellulase and hemicellulase. Destroying the lignocellulose structure needs the combined action of various lignocellulose enzyme systems, and after the five strains are mixed, the synergistic effect among the enzyme systems in the system can be remarkably enhanced, the lignocellulose structure is efficiently destroyed, and the degradation rate of the wood fiber raw material is increased.

The invention discloses a kind of eucommia leaf fermentation extract and its preparation method and application, and belongs to the technical field of microorganisms and feed additives. With eucommia leaf and wheat bran as substrates, by configuring a specific black aspergillus niger-trichoderma harzianum-saccharomyces cerevisiae complex bacterial liquid as a fermentation agent, a closed fermentation is carried out to make black aspergillus, trichoderma harzianum, and saccharomyces cerevisiae ferment to produce a large amount of cellulase, protease, lipase, amylase and glucosidase, etc., and then by pumping and circulating treatment, the cellulose, protein, starch, beta-glycan and other macromolecular substances in eucommia leaf are further enzymolyzed into protein oligopeptides, monosaccharides, and soluble oligosaccharides that are beneficial to livestock and poultry absorption. The eucommia leaf fermentation extract prepared by the present invention is used as a feed additive for beef cattle feeding, improves the daily weight gain and feed intake of beef cattle, and significantly reduces the feed-to-meat ratio, saves feed consumption, improves the protein content in meat, reduces the fat content, reduces energy, and improves meat quality.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses a method for preparing an agricultural biostimulant containing fructooligosaccharide from molasses and application of the agricultural biostimulant, and relates to the technical field of agricultural planting of fruits. The agricultural biostimulant is prepared by fermenting cane molasses with aspergillus oryzae or aspergillus niger, and the specific fermentation method comprises the following steps: inoculating activated strains into a cane molasses diluent with the baume degree of 10-16oBX, and carrying out aerobic fermentation on a shaking table at 25-35 DEG C for 70-72 hours to obtain a strain seed solution; and inoculating the strain seed solution into cane molasses diluent with the baume degree of 20-80 oBX, carrying out aerobic fermentation on a shaking table at 40-55 DEG C for 70-72 hours, and sterilizing to obtain the strain. By controlling the types of fermentation strains, the baume degree of cane molasses, the fermentation temperature and the fermentation time, the obtained fermentation product has the effects of promoting the growth and development of crops and increasing the yield, and is suitable for popularization.

The invention belongs to the technical field of agricultural biology, and particularly relates to a compound bacterial fertilizer with a soil structure repairing function and a preparation method thereof. The three-layer embedding structure is more beneficial to improving the survival rate of strains, the limitation of traditional single-layer embedding is broken through through the three-stage structure design of inner core slow release, middle layer regulation and control and shell adsorption, integration of salt adsorption, structure improvement and flora colonization is achieved through cooperation of multiple materials including PGA, biochar and montmorillonite, and saline-alkali soil can be well repaired and improved. Besides, compared with conventional Aspergillus niger, the Aspergillus niger GRJC004 provided by the invention is suitable for survival in a saline-alkali environment, and is especially suitable for restoration and improvement of saline-alkali soil in combination with the three-stage structural design of the Aspergillus niger GRJC004.

The invention discloses a ganoderma lucidum edgeworthia chrysantha promoting agent for promoting efficient edgeworthia chrysantha of aquilaria plants, and belongs to the technical field of edgeworthia chrysantha of aquilaria plants. The component A is prepared from the following components in parts by weight: 20 to 40 parts of aspergillus niger microbial fermentation liquor, 15 to 30 parts of verticillium fulvum microbial fermentation liquor, 10 to 15 parts of fusarium oxysporum microbial fermentation liquor, 5 to 10 parts of ganoderma lucidum extract, 6 to 8 parts of seaweed extract, 2 to 8 parts of malic acid, 3 to 6 parts of fructose, 1 to 3 parts of pectinase and 0.5 to 2 parts of cellulase. Through the synergistic effect of the component A and the component B, the agilawood formation rate and quality of agilawood are effectively improved, the synergistic effect of microorganisms stimulates the agilawood formation potential of plants, accurate regulation and control of the plant hormone xylan emulsion provide conditions for synthesis and accumulation of agilawood substances, and compared with a traditional agilawood formation method, the agilawood formation method has the advantages that the agilawood formation rate is increased, and the agilawood formation quality is improved. According to the method, the agilawood with higher quality can be obtained within shorter time, and the yield is increased.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention relates to the technical field of soil remediation, and provides a soil pollution remediation process which comprises the following steps: distributing points in grids of an area to be remediated, measuring the content of heavy metals and the concentration of organic pollutants, applying multi-frequency microwave radiation, synchronously applying ultrasonic treatment, and injecting a complex microbial inoculant containing sphingomonas, aspergillus niger spores and a nitrogen and phosphorus nutrient solution. A direct-current electric field is applied, pH, oxidation-reduction potential Eh and temperature parameters are monitored and measured in real time through an embedded sensor, a repair by-product recovery system is arranged, the purity of recovered metal is not lower than 98%, and mobile repair equipment is used in a matched mode. According to the method, the pollution degree is accurately quantified by constructing the pollution index PI, and dynamic regulation and repair are realized in combination with real-time monitoring parameters of a sensor. The pollutant removal efficiency is improved through the synergistic effect of multiple technologies, metal resource recovery is achieved through a repair by-product recovery system, traditional problems are solved, and efficient and environment-friendly repair is achieved.

The present invention belongs to the technical field of biomass resource utilization and microbial fertilizers, and specifically relates to a fertilizer based on oil-tea camellia cattail, a preparation method and its application. The fertilizer described in the present invention is prepared by a collaborative fermentation process of Aspergillus niger, Phanerochaete chrysosporium and Bacillus subtilis. The obtained fermentation product is separated into solid and liquid to obtain a solid, the solid being a solid fertilizer, and the liquid being a foliar fertilizer after post-treatment. Both can be used for the cultivation of crops such as tangerines and oil-tea camellias. Based on the microbial collaborative fermentation process, the content of anti-nutritional factors tannin and tea saponin in the obtained solid fertilizer is significantly reduced, which is less than 1.8 g / kg and 0.1% respectively on a dry basis, while maintaining a seed germination index higher than 95%. According to a planting comparative test, the average yield per mu of oil-tea camellias using the solid fertilizer and liquid fertilizer obtained in this application increased by 12%. The average yield per mu of tangerines increased by about 11%, and the sugar, vitamin C, and trace element contents in tangerines were higher than those using traditional fertilizers.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention relates to the technical field of Chinese herbal medicine fermentation, and discloses a mixed strain fermentation method for efficient conversion of non-medicinal parts of traditional Chinese medicine, which comprises the following steps: constructing an intelligent fermentation system comprising a plurality of independent fermentation units, respectively culturing different strains such as aspergillus niger, bacillus subtilis, saccharomyces cerevisiae and the like in each fermentation unit, the online sensor is integrated in the system and used for monitoring key fermentation parameters in real time, and a fermentation model and an optimization algorithm are arranged in the system and can automatically adjust fermentation conditions. An intelligent fermentation system comprising a plurality of independent fermentation units is constructed, precise classification pretreatment and mixing according to a specific ratio are carried out on rhizoma polygonati stems and leaves and gentian stems and leaves, and an intelligent control system is utilized to dynamically regulate and control the fermentation process, so that the synergistic effect of different strains such as aspergillus niger, bacillus subtilis and saccharomyces cerevisiae is fully exerted, and the fermentation efficiency is improved. The dissolution rate and the conversion rate of active ingredients in the non-medicinal part of the traditional Chinese medicine are improved.

The invention discloses coprinus cinereus sourced laccase as well as a recombinant expression strain and a high-efficiency expression method thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, aspergillus niger MA70.15 is used as an experimental strain, and a random integration strategy is adopted, so that a pC3-PcitA-SPGlaA-lcc9-TtrpC-pyrG expression vector is integrated in an upstream region of a promoter of host bacteria citrate synthase in a form of nine continuous copies. In order to explore the influence of particles and osmotic regulators in a fermentation culture medium on laccase production through fermentation of recombinant aspergillus niger, the particles and osmotic regulators of different types and concentrations are added, so that the enzyme activity of the laccase fermented in a shake flask of the recombinant strain AnRcgL1 reaches 1566.7 U / L, the enzyme activity fermented in a 1L fermentation tank reaches 1961 U / L, and the recombinant high-efficiency expression of the laccase Lcc9 in the aspergillus niger is successfully realized.

The invention provides a method for preparing quintuple diglyceride oil for improving blood vessel health through enzymolysis, application and a product, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, and carrying out enzymolysis reaction, molecular distillation, decoloration and deodorization to obtain diglyceride oil; the lipase is a mixture of Candida antarctica Aspergillus niger lipase, Candida column lipase and bovine pancreas lipase, and the weight ratio of the low-erucic acid rapeseed oil to the glycerin to the water to the fatty acid to the lipase is 500: (80-100): (1-2): (30-60): (10-20). The diglyceride oil prepared by the method can be used for preparing a diglyceride oil composition, and has five functions of reducing blood uric acid, reducing blood total cholesterol, reducing blood triglyceride, reducing blood sugar and increasing blood flow velocity to improve blood vessel health.

The invention provides a conditioner for soil heavy metal pollution remediation and a preparation method thereof, and belongs to the technical field of soil conditioning. The conditioner for soil heavy metal pollution remediation provided by the invention is prepared from the following components in parts by mass: 12 to 25 parts of biochar, 5 to 7 parts of potassium feldspar, 1 to 5 parts of inorganic nano material and 40 to 70 parts of water, the fertilizer is prepared from 40-50 parts of humic acid, 15-25 parts of amino acid, 5-12 parts of citric acid, 4-8 parts of bagasse and 25-40 parts of vinasse. 0.5 to 1 part of aspergillus niger and 0.1 to 1 part of clostridium capsulatum. By utilizing the adsorption of inorganic components on heavy metal elements, the improvement of organic components on saline-alkali soil and the continuous remediation effect of microbial components on the soil, through the synergistic effect of the multiple components, the soil is treated at the same time from multiple aspects of soil fertilization, plant growth, soil loosening, salinity and alkalinity reduction and the like; the soil salinity and alkalinity are reduced, and heavy metals are adsorbed and absorbed.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.

The invention provides a recombinant Aspergillus niger strain with high yield of trans-aconitic acid, and belongs to the technical field of gene engineering. The recombinant aspergillus niger strain can simultaneously express aconitase and aconitic acid isomerase. According to the genetic engineering aspergillus niger strain provided by the invention, the trans-aconitic acid fermentation yield is generally higher than that of an original strain, and the strain with the highest yield can reach 51g / L on a shake flask level, so that the genetic engineering aspergillus niger strain has very high application value.