3031 results about "Aspergillus niger" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention discloses an organic matter decomposing inoculant, and a preparation method and application thereof. The organic matter decomposing inoculant comprises the following ingredients in part by weight: 10 to 20 parts of aspergillus niger microbial inoculum, 10 to 20 parts of lactobacillus plantarum microbial inoculum, 20 to 30 parts of saccharomyces cerevisiae microbial inoculum and 40 to 60 parts of bacillus subtilis microbial inoculum. The organic matter decomposing inoculant mainly comprises various microbial floras with mutual promotion and mutual synergy effects. The organic matter decomposing inoculant can decompose various organic matters quickly, can effectively kill germs, ova and weed seeds in the decomposed raw materials, can reduce diseases, weed and insect pests, andcan remove water and odor. The organic matter decomposing inoculant can stimulate growth and development of crops, can improve disease resistance, drought resistance and cold resistance, can increasesoil nutrients, can improve the soil structure, and can increase the utilization ratio of chemical fertilizer. The organic matter decomposing inoculant can be used directly and can also be used for producing commercial organic fertilizer, biological organic fertilizer, organic and inorganic complex fertilizer, biological organic and inorganic complex fertilizer and the like.

The invention relates to a production technology of black tea, in particular to the technical field of the black tea. The production technology of the black tea comprises the following working procedures: green removing, twisting, piling, drying at first time, steaming, compacting, fermenting and drying 2, wherein pulp filling is carried out on a tea leaf raw material before the green removing working procedure, and the mass ratio of water to tea leaf is 6-9% in the pulp filling process; and artificially adding inoculated strains between the steaming working procedure and the fermenting working procedure, wherein the artificially inoculated strains are one or more of Eurotium cristatum, candida albican and aspergillus niger. By adopting the production technology provided by the invention,time of a fermentation technology in the production process of the black tea is reduced, production efficiency is improved, and yield of the black tea is increased; meanwhile, the problem that the traditional black tea production technology is controlled by experience and level of a tea making master worker is solved, the time for producing the black tea by utilizing the technology is short, the produced black tea has good consistency, strong taste, pure fragrance, red-yellow soup colour and brown and bright leaf edge.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The invention discloses microbial straw decomposition additive, which belongs to the field of straw decomposition additive and is obtained through combining four bacteria: bacillus subtilis, aspergillus niger, trichoderma reesei and saccharomyces cerevisiae. The microbial straw decomposition additive utilizes the temperature rise benefit of the aspergillus niger spore enzyme activity for raising the environment temperature of decomposition, the further temperature rise benefit in the growth process of the bacillus subtilis is cooperated, and the temperature required by the decomposition is ensured, so the decomposition effect of straws is improved. The microbial straw decomposition additive has the obvious temperature rise benefit and can be used for the in-site field returning decomposition of the straws, the straw decomposition can be better accelerated in low-temperature and normal-temperature environment, the bottleneck problem of straw utilization is solved, the straw burning can be effectively reduced, the decomposition work procedure and the labor cost are saved, the fertilizer feeding is reduced, the soil structure is improved, and the planting benefit is improved.

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

The invention discloses microbial ecological organic fertilizer and a preparation method thereof. The organic chemical fertilizer comprises the following components: edible mushroom dregs, sugarcane dregs, peat soil, oil squeezed waste, filter sludge, livestock and poultry manure and a microbial agent, wherein the microbial agent comprises bacillus subtilis, saccharomycetes, aspergillus niger, guanyloribonuclease, trichoderma, lactobacillus, photosynthetic bacteria, actinomycetes and streptomyces microflavus. The prepared organic fertilizer has advantages of wide raw material source, low cost, high absorption utilization rate, comprehensive nutrients, low dosage, no odor, fast fertilizer effect, various bacteria types, high content, capability of preventing injurious pests, simple preparation method and the like.

The invention relates to a Bacillus subtilis T-500. The Bacillus subtilis T-500 has been preserved in CGMCC (China General Microbiological Culture Collection), and the preservation number is CGMCC NO:6058. The Bacillus subtilis T-500 can be used as a biological pesticide for preventing and treating multiple plant diseases, and can also be used as a biological fertilizer for promoting the yield increase of crops. The Bacillus subtilis T-500 has higher antibacterial activity on Magnaporthe oryzae, Pellicularia sasakii, Xanthomonas oryzae, Ustilaginoidea virens, Xanthomonas oryzae pv. oryzicola, Fusarium graminearum, Aspergillus flavus, Aspergillus niger and other pathogenic bacteria, and has better prevention and control effects on diseases caused by the pathogenic bacteria. The Bacillus subtilis T-500 can prevent and treat rice leaf diseases when being diluted 200-500 times on the leaves of rice, can prevent and treat bakanae diseases of rice after seed soaking, and can prevent and treat soil-borne diseases of vegetables and the like after root irrigation.

The subject invention pertains to polynucleotides encoding the enzyme oxalate decarboxylase from the filamentous fungus Aspergillus niger and methods of use. The subject invention also pertains to methods of using the enzyme oxalate decarboxylase from Bacillus subtilis.

The invention relates to an antibacterial mildewproof polylactic acid composition and a preparation method thereof. The composition comprises the following mixed components: polylactic acid, an antibacterial mildewproof agent, and a dispersant; wherein on a basis of 100 parts by weight of polylactic acid, the composition comprises 0.3-2 parts by weight of antibacterial mildewproof agents, and 0-0.5 parts by weight of dispersants; the antibacterial mildewproof agent is a polyguanidine pyrithione antibacterial mildewproof agent which is obtained by mixing an aqueous solution of water-soluble polyguanidine inorganic acid salts or organic acid salts and an aqueous solution of sodium pyrithione; the molar ratio of the water-soluble polyguanidine inorganic acid salts or organic acid salts and sodium pyrithione is 1:(0.1-5). The polylactic acid composition of the invention has good antibacterial and mildewproof effect. The antibacterial effect on staphylococcus aureus and escherichia coli is up to 99.9%; the mildewproof grade for mold such as aspergillus niger, aspergillus terreus, paecilomyces varioti bainier, and the like reaches 0 grade; the composition has good water resistant stability, and has wide application prospects.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu/g; lactobacillus acidophilus is not less than 1.0*10cfu/g; lactobacillus plantarum is not less than 1.0-10cfu/g; bacillus natto is not less than 1.0*10cfu/g; aspergillus niger is not less than 1.0*10cfu/g; and candida utili is not less than 1.0*10cfu/g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention discloses a meat chick granulated feed containing an active bacterium fermentation ingredient and a traditional Chinese medicine ingredient. The meat chick granulated feed comprises 5-15 percent of the active bacterium fermentation ingredient, 0.3-1 percent of the traditional Chinese medicine ingredient and the balance of a full value chicken feed. The active bacterium fermentation ingredient is prepared by adopting the following method of: adding an aspergillus niger culture solution, a probiotics culture solution and a yeast culture solution to a fermentation medium respectively according to an inoculation quantity of 5-15 percent for fermentation comprising primary anaerobic fermentation and secondary aerobic fermentation; and after the fermentation, carrying out low-temperature drying at 55 DEG C and smashing on the culture solution to obtain the active bacterium fermentation ingredient. Proved through tests, the feed provided by the invention can be used for obviously improving the food consumption and the daily gain of meat chicken and reducing the feed conversion ratio and the diarrhea rate, does not contain antibiotics, has no toxic and side effects and environmental pollution and can be used for providing high-quality green livestock and poultry products.

The invention discloses a method for producing fermented bean curd through multi-strain mixed fermentation. The method comprises the following steps of: (1) preparing raw fermented bean curd, namely preparing the raw fermented bean curd from soybean; (2) culturing bacteria at the early stage, namely inoculating mixed strains comprising mucor, Aspergillus oryzae and Aspergillus niger into the raw fermented bean curd to culture bacteria at the early stage to obtain the fermented bean curd blanks; (3) pickling by using salt, namely placing the fermented bean curd blanks obtained in the step (2) into a rubber box, scattering salt layer by layer, and pickling with the salt to prepare salty blanks; (4) fermenting at the later stage, namely placing the pickled fermented bean curd salty blanks into a bottle, inoculating flavor-increasing yeast into wine liquid of semifinished products, and fermenting at the later stage; and (5) obtaining the fermented bean curd. By the method, the flavor of the fermented bean curd products can be enriched and the fermenting period of the fermented bean curd is shortened.

A process for preparing the multi-vitamine multi-enzyme high-protein microbial preparation by the solid fermentation of multiple bacterial strains includes such steps as preparing seed culture media, respectively inoculating reticularia, torula yeast, glutamic corynebacteria, Bacillus subtitis, Aspergillus niger, etc into said seed culture media, culturing at 30-35 deg.C for 15-18 hr, inoculating them into the solid culture medium, fermenting at 33+/-0.5 deg.C for 70-80 hr, and fast drying. Its can be used as the feed containing living microbes, microbial enzymes, vitamines, etc.

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r/min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U/L of Lip, 11U/L of MnP and 5.6U/L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.