457 results about "Laccase" patented technology

The invention relates to the technical field of carbon black, in particular to a preparation process of high-adsorbability carbon black, which comprises the following steps: performing pore-forming treatment on carbon black to obtain a porous carbon black material, then treating the porous carbon black material with a first mixed amino acid solution to obtain a carbon black material loaded with a first mixed amino acid adsorption layer, and drying the carbon black material to obtain the high-adsorbability carbon black. And treating the carbon black material with a second mixed amino acid solution to obtain a carbon black material loaded with a second mixed amino acid adsorption layer, and treating the carbon black material loaded with mixed amino acids with laccase and tyrosinase. The carbon black product obtained by the preparation process has high adsorbability, and is used for treating metal ions in wastewater, so that the removal rate of the metal ions is greatly improved.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

The invention discloses a laccase mutant and application thereof in desizing wastewater treatment. Wild laccase is used as a female parent, and a high-enzyme-activity mutant is obtained through multiple groups of mutations. The temperature of the desizing wastewater subjected to centralized treatment is 15-50 DEG C, the pH value is 4-7, and the application condition is wide. Through verification, the enzyme activity of the laccase mutant disclosed by the invention is more than 10U / mg under the environment that the temperature is 25 DEG C and the pH value is 6.0 (common conditions for centralized treatment of desizing wastewater). Even under the extreme conditions that the pH is 4.0 or 7.0 and the reaction temperature is 15 DEG C or 50 DEG C, the laccase variant can still keep the enzyme activity of 5.5 U / mg or above, and therefore the laccase variant can be used for treating desizing wastewater with the temperature being 15-50 DEG C and the pH being 4-7 in a large scale. Compared with wild type laccase with lower enzyme activity, the laccase mutant obtained by the invention has the advantages that the enzyme activity is obviously improved and can be improved by 10 times at most, and the wastewater treatment cost is obviously reduced.

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention belongs to the technical field of gene engineering of enzymes, and particularly relates to high-temperature-resistant laccase as well as preparation and application thereof. According to the present invention, the laccase gene derived from Priesta aryabhattai is subjected to molecular modification through the rational design so as to obtain the S281E mutant and the S281E / N387D mutant; the enzyme activities of the mutants S281E and S281E / N387D are respectively improved by 72.7% and 40.4% compared with the enzyme activities of the wild type mutants S281E and S281E / N387D; under the conditions that the temperature is 70 DEG C and the temperature is kept for 60 minutes, the residual enzyme activity of WT is 25.2%, the residual enzyme activity of S281E is 45.1%, and the residual enzyme activity of S281E / N387D is 80.2%.

The invention discloses a laccase mutant and application thereof in oxidative conversion of aflatoxin. According to the invention, laccase of coprinus cinerea is used as a starting enzyme, and through molecular docking, a structure-function relationship is analyzed, and mutation is designed. After induced expression of engineering bacteria containing mutant genes, mutant laccase Lcc5-T485E with improved detoxification efficiency is obtained through screening. When the enzyme with the same protein mass is used, the AFB1 conversion efficiency of the mutant Lcc5-T485E is obviously improved compared with that of Lcc5, and the mutant enzyme has potential application value in oxidative conversion of aflatoxin.

The invention provides an apple laccase LAC3 gene and application thereof in improving aphid resistance of plants, the apple laccase LAC3 gene is cloned, a nucleotide sequence and a protein structure of the apple laccase LAC3 gene are clarified, a primer is designed on the basis, an overexpression vector of the apple laccase LAC3 gene is constructed, and the overexpression vector is transferred into tobacco through tobacco tissue culture seedling culture and agrobacterium mediation to obtain a transgenic plant. Homozygote strains are obtained through screening, aphid feeding behavioral observation is carried out through an EPG technology, aphid inoculation research is carried out, and the aphid resistance function of the aphid strains is clarified. Compared with a wild plant, the growth vigor density of the overground part of the transgenic plant is larger than that of the wild plant, stalks are thick and strong, lignin is increased, a good resistance effect on aphids is achieved, and a theoretical basis is provided for aphid-resistant molecule improved breeding.

The invention discloses a laccase mutant and application of the laccase mutant in preparation of polyflavone by oxidative conversion of daidzein. According to the invention, laccase Lcc5 of coprinus cinerea is used as a starting enzyme, and through molecular docking, a position structure is analyzed, and mutation is designed. Synthesizing a mutant gene, and constructing a recombinant expression engineering bacterium of the mutant gene. After engineering bacteria containing mutant genes are subjected to induced expression, the Lcc5 mutant Lcc5-T485E is obtained through separation and purification, the specific enzyme activity and ethanol tolerance of the mutant are improved, and the conversion rate of daidzein is increased to about 1.26 times under the same enzyme activity; the antioxidant activity, the enzyme inhibition activity and the antibacterial activity of the obtained conversion product are also remarkably improved. The mutant enzyme has potential application value in oxidative conversion of daidzein and improvement of biological value of daidzein.

The invention discloses a supported ozone catalyst for printing and dyeing wastewater treatment and a treatment process, and relates to the technical field of wastewater treatment, the supported ozone catalyst comprises an environmental response type porous medium, an active center and a biological enzyme modification layer; the environment-responsive porous medium is based on poly (N-isopropylacrylamide) grafted and modified mesoporous alumina, the initial aperture of the mesoporous alumina is 10-15nm, the grafting rate of poly (N-isopropylacrylamide) is 12-20%, and an active center is composed of a transition metal active center and quantum dots modified on the transition metal active center. The biological enzyme modified layer is obtained by carrying out deep biological modification on the surface of the catalyst by using laccase with oxidation-reduction activity. According to the invention, an environment-responsive porous medium is constructed by grafting mesoporous alumina with poly (N-isopropylacrylamide), so that the problems that a traditional fixed-aperture catalyst is low in mass transfer efficiency and poor in adaptability to working conditions, and the pollutant release efficiency is difficult to effectively improve under different temperature and pH conditions are solved.

The invention belongs to the technical field of gene engineering, and relates to high-temperature-resistant laccase, a gene and application of the high-temperature-resistant laccase in mycotoxin degradation. The amino acid sequence of the laccase TtLac-4 is as shown in SEQ ID NO: 1. On the basis of wild laccase, site-directed mutagenesis modification is carried out through artificial rational design, and an amino acid sequence of the modified high-temperature-resistant laccase and a nucleotide sequence of the artificially optimized high-temperature-resistant laccase gene are obtained. The high-temperature-resistant laccase obtained through gene coding of the high-temperature-resistant laccase has the remarkably-improved high-temperature-resistant characteristic, and the optimum temperature reaches 90 DEG C. The degradation rate of the laccase TtLac-4 obtained by the invention on mycotoxin aflatoxin B1 and zearalenone reaches 100%. The invention lays a foundation for a green degradation process and application of mycotoxin degradation.

The invention discloses coprinus cinereus sourced laccase as well as a recombinant expression strain and a high-efficiency expression method thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, aspergillus niger MA70.15 is used as an experimental strain, and a random integration strategy is adopted, so that a pC3-PcitA-SPGlaA-lcc9-TtrpC-pyrG expression vector is integrated in an upstream region of a promoter of host bacteria citrate synthase in a form of nine continuous copies. In order to explore the influence of particles and osmotic regulators in a fermentation culture medium on laccase production through fermentation of recombinant aspergillus niger, the particles and osmotic regulators of different types and concentrations are added, so that the enzyme activity of the laccase fermented in a shake flask of the recombinant strain AnRcgL1 reaches 1566.7 U / L, the enzyme activity fermented in a 1L fermentation tank reaches 1961 U / L, and the recombinant high-efficiency expression of the laccase Lcc9 in the aspergillus niger is successfully realized.

The invention provides a modification method of a ramie fabric, which comprises the following steps: sequentially treating and modifying the ramie fabric by three stages of pectinase, complex enzyme comprising xylanase, hemicellulase, cellulase and laccase, and alkaline protease, so that hairiness on the surface of the ramie fabric is greatly reduced, and the scratchiness is greatly reduced. The wearing experience feeling of the textile taking the ramie fabric as the raw material is greatly improved.

The invention belongs to the technical field of nano-enzymes, and particularly relates to a CuPH (at) KMO composite nano-enzyme material as well as a preparation method and application thereof. Aiming at the problems of poor catalytic activity, complex steps, high raw material cost and low biocompatibility of nano-enzyme prepared by the existing method, the preparation method comprises the following steps: by taking terephthalic acid and histidine as dual-coordination copper-based nano-enzyme as a precursor, introducing manganese oxide in situ under a hydrothermal condition; and the flower-like nano mimic enzyme which is uniform in size and has high laccase-like catalytic characteristics at the same time is synthesized. The composite nano-enzyme provided by the invention can catalytically oxidize phenol amine organic compounds under the condition that the pH value is 3-9, can realize the detection of the content of ergothioneine in cosmetics and nutritional supplement capsules, and can be applied to the field of cosmetic and food safety monitoring.

The invention discloses a method for jointly degrading a dicarboximide bactericide through a bacterium-enzyme medium system. According to the method, Pseudomonas sp. F13, laccase and a laccase medium are adopted for jointly degrading the dicarboximide bactericide and metabolites of the dicarboximide bactericide. The preservation number of the pseudoarthrobacter sp. F13 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20241866, and the preservation number of the pseudoarthrobacter sp. F13 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20241866. Experimental results show that under the condition that the pH is 4.8 and 6.0, 100% degradation of the dicarboximide fungicide can be achieved through the method, accumulation of 3, 5-DCA is not detected, the method provides a new way for degradation of procymidone and toxic intermediate metabolite 3, 5-DCA, and the method is simple, easy to operate and suitable for market popularization and utilization.

The invention relates to a method for determining potassium by using a nano-enzyme-based colorimetric probe, and the probe and application thereof. The invention relates to a method for determining potassium by using a nano-enzyme-based colorimetric probe, which comprises the following steps of: synthesizing copper and cerium-containing phosphotungstic acid nano-enzyme by using phosphotungstic acid, cerous nitrate and copper chloride as precursors through a hydrothermal method, detecting the absorbance of K < + > standard solutions with different concentrations by using the copper and cerium-containing phosphotungstic acid nano-enzyme as a probe, establishing a quantitative relation between the absorbance and the K < + > concentration, drawing a standard curve, obtaining a regression equation, and determining the potassium content by using the regression equation. And detecting the absorbance of the sample determination standard solutions with different concentrations, and obtaining the K < + > content of the sample through the regression equation. According to the method, the nano-enzyme (Cu, Ce-PWs) is prepared by using tungsten-molybdenum heteropoly acid with high negative charges, unique chelating properties and various structures and Ce < 4 + > and Cu < 2 + > as raw materials, strong mimic enzyme laccase activity is generated through the synergistic effect of Ce atoms and Cu atoms, and the activity of the mimic enzyme laccase is further improved in the presence of H2O2.

The invention relates to the technical field of food processing, in particular to a chicken premade dish and a preparation method thereof. By innovatively integrating a laccase-chequer-shaped indocalamus leaf cross-linking system and a double-frequency ultrasonic pretreatment process, the water retention, bacteriostasis and flavor regulation performance of the chicken premade dish can be synchronously improved, and the effect cannot be replaced by other enzymes such as papain; besides, double-frequency ultrasonic pretreatment is adopted, dynamic balance of deep penetration-surface tenderization is achieved through frequency alternation, and compared with single-frequency or traditional soaking, the method can better cooperate with laccase catalysis to directionally cross-link the preservative, and the effects of water retention, bacteriostasis and flavor regulation and control are achieved.

The present invention provides a azelastine hydrochloride nasal spray and a preparation method thereof, belonging to the technical field of nasal sprays. The azelastine hydrochloride nasal spray is composed of the following components by mass percentage: 0.1% of azelastine hydrochloride, 0.3 - 0.4% of a composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower, 0.005 - 0.01% of a stabilizer, 0.002 - 0.01% of a preservative, 0.95 - 1.05% of a thickening agent, 0.7 - 0.8% of a pH regulator, 0.45 - 0.48% of sodium chloride, and the balance of purified water; the composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower is prepared by first subjecting a mixture of chamomile, houttuynia cordata, and peony flower to water extraction to obtain a water extract and then subjecting it to composite enzyme fermentation. The composite enzyme includes cellulase, pectate lyase, and laccase. The present invention can effectively reduce the irritation to the eyes during use and ensure safer and more reassuring use.

The application discloses a kind of fungal laccase mutant Lcc5-G and its expression strain and application.The application is based on the fungal laccase from Coprinopsis cinerea heterologous expression since, by site-directed mutagenesis, obtain mutant gene.After fungal induction expression containing mutant plasmid, obtain laccase mutant enzyme Lcc5-G with improved specific activity, stability and AFB1 removal efficiency.When ABTS is used as substrate, the specific activity of the mutant is improved by 3 times, and the stability of the mutant enzyme is improved to 5 times of the original enzyme at 45 DEG C and pH 7.The mutant has potential application value in oxidizing aflatoxin.

The invention relates to the technical field of sewage treatment, and particularly discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The invention discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The high-suspended-matter sewage treatment agent comprises 40-60 parts of modified bentonite; 5-15 parts of ferrous sulfate; 5-15 parts of aluminum ferric sulfate; 1 to 5 parts of ethylenediamine tetraacetic acid; 10 to 20 parts of an active biochar material; 3 to 8 parts of chitosan; 5 to 10 parts of a flocculating agent; the modified bentonite is obtained by modifying bentonite through laccase and nano zero-valent iron; the active biochar material is obtained by taking waste biomass as a raw material and carrying out semi-carbonization and activation treatment. The sewage treatment agent disclosed by the invention can be used for purifying high-suspended-matter sewage generated by oil extraction plants and oil wells, has high removal efficiency on suspended matters, and can effectively remove organic humus, so that the content of the suspended matters in the treated sewage reaches the standard.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The invention discloses a microbial decomposing inoculant for straws and a preparation method thereof, and belongs to the technical field of microorganisms. The microbial decomposing inoculant for straw is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of a nano-porous diatomite carrier, 5-7 parts of a humectant, 2-3 parts of humic acid chelated trace elements and 2-4 parts of an inducer. The preparation method comprises the following steps: screening talaromyces thermophilus, Guangxi thermoactinomycetes and arthrobacter habitualis to form a functional microbial liquid, adding a laccase-protease compounding system to cooperate with the physical adsorption effect of diatomite, and adding humic acid chelated trace elements to form an ion exchange network with polyglutamic acid; straw components are efficiently degraded through a triple mechanism of'enzymatic degradation-element supplementation-microenvironment regulation and control ', the decomposition process is accelerated, and the straw compost quality is improved.

The present disclosure provides a porous microcarrier loaded with laccase, a preparation method and an application. The porous microcarrier loaded with laccase includes: a hydrogel porous microcarrier; laccase, which is loaded on the hydrogel porous microcarrier by covalent bonding; wherein the hydrogel porous microcarrier is a methacrylated hydrogel microcarrier filled with chitosan, providing a loading site for laccase.

The invention belongs to the technical field of biology, and particularly discloses application of laccase AmLac in degradation of biogenic amines. The amino acid sequence of the laccase is shown as SEQ ID NO.1. The laccase has different degrees of degradation effects on the biogenic amines such as histamine, spermidine, tyramine, phenylethylamine and putrescine, and the degradation rates of the histamine and the putrescine both reach 100%. The enzyme is stable in enzymatic property, has alcohol tolerance and acid tolerance, has certain adaptive capacity to food matrixes, and can effectively degrade biogenic amines in wine and reduce potential hazards of the biogenic amines to human bodies, so that the safety and stability of products are improved. Therefore, the AmLac has a wide application prospect in the food industry, and especially when the AmLac is used for treating food containing high biogenic amine, the safety quality of the food can be remarkably improved.

The invention discloses a preparation method and application of immobilized laccase at room temperature, and the preparation method comprises the following steps: step 1, weighing laccase, dissolving the laccase in a sodium acetate-acetic acid buffer solution, carrying out magnetic stirring, and then adding a sodium hydroxide solution containing trimesic acid to form a mixed solution; step 2, adding an aqueous solution containing ferric ions into the mixed solution, magnetically stirring, standing and aging in a refrigerator, and centrifugally collecting a solid product; and step 3, re-suspending the washed precipitate in a polyvinylpyrrolidone aqueous solution, fully dispersing, and then drying to obtain the immobilized laccase, which is recorded as Lacc-Fe-BTC. The immobilized laccase prepared by the invention can be used for rapidly and efficiently degrading aflatoxin B1 and zearalenone, and recycling and reutilization of the enzyme after reaction can be realized.

The embodiment of the invention provides a fluorescent probe, an application method thereof and a fluorescent probe kit. According to the fluorescent probe provided by the embodiment of the invention, the laccase has relatively high sensitivity and specificity, and the laccase can be accurately detected under the condition that the laccase concentration is relatively low, so that the laccase detection accuracy is effectively improved. When the fluorescent probe provided by the embodiment of the invention is used for laccase detection, the detection cost can be reduced to 1 yuan per time, and compared with the existing ABTS colorimetric method, the cost advantage is better.

The invention relates to high-strength degradable self-healing hydrogel and a preparation method thereof, and belongs to the technical field of high polymer materials. The hydrogel is prepared from the following components in percentage by mass: 15 to 20 percent of carboxymethyl cellulose, 10 to 14 percent of acrylic acid, 15 to 20 percent of N-isopropylacrylamide, 4 to 8 percent of tannic acid, 2 to 4 percent of borax, 0.06 to 0.1 percent of laccase, 0.5 to 1 percent of N, N-methylene bisacrylamide, 0.4 to 0.6 percent of potassium persulfate and the balance of phosphate buffer solution. According to the hydrogel disclosed by the invention, through dual-network structure design, dynamic covalent bond crosslinking and enzyme catalysis green synthesis, collaborative optimization of high strength, high self-healing property, degradability and multi-responsiveness is realized, and the problems of poor mechanical property, low repairing efficiency, environmental unfriendliness and the like of traditional hydrogel are solved; the method has wide application potential in the fields of biomedicine, flexible electronics, intelligent packaging and the like.

The invention discloses a method for brewing high-quality antioxidant Luzhou-flavor liquor in a semi-solid state. The method comprises the following steps: separating glutinous sorghum serving as a wine brewing raw material into sorghum rice and sorghum peel through rice milling, recovering residual starch in the sorghum peel by adopting alpha-amylase and saccharifying enzyme, releasing different tannins by adopting cellulase, and depolymerizing tannin dissolved in the sorghum peel and a solution by adopting laccase to release tannin with different structures, so as to obtain tannin enzymatic hydrolysate; the enzymatic hydrolysate is added into the steamed peeled sorghum rice according to a certain proportion, so that the thermal destructive effect of the steamed material on tannin in the sorghum rice is avoided; and adding yeast for making the white spirit in a semi-solid state to obtain the white spirit which has the antioxidant activity exceeding 80% compared with that of the existing white spirit and meets the national standard of semi-solid Luzhou-flavor white spirit. Compared with the traditional baijiu brewed by steaming sorghum, the total ester content of the baijiu brewed by adding the tannin enzymatic hydrolysate is increased by 87.50-102.27%, the free radical scavenging rate of DPPH is increased by 134.16-158.71%, and the content of guaiacol substances (4-ethyl guaiacol and guaiacol) is increased by 160.69-166.90%.