36 results about "Laccase" patented technology

This invention discloses a laccase mutant, Lcc5-I479T, its expression strain, and its applications. Using laccase from *Coprinopsis cinerea* as the starting enzyme, this invention obtained the mutant gene through molecular docking and positional analysis. After induction expression in engineered bacteria containing the mutant gene, the stable laccase mutant enzyme Lcc5-I479T was obtained. Using ABTS as a substrate, the mutant enzyme Lcc5-I479T showed a stable increase in activity to 8-20 times that of the starting enzyme at 35-50℃. This mutant enzyme has potential application value in the oxidative transformation of aflatoxin.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

This invention relates to the field of food processing, and provides an enzyme-mediated enzymatic treatment method for olive de-astringency and polyphenol conversion, solving the problems of high pollution from chemical methods and severe browning from traditional enzymatic methods in existing olive de-astringency technologies, resulting in poor product appearance, strong astringent taste, and loss of nutritional active ingredients. The method includes the following steps: S1, preparing an olive raw material slurry; S2, adding laccase to the olive raw material slurry, adding a citrate-sodium citrate buffer solution to adjust the reaction environment, and then adding a color-protecting agent to obtain a first solution system; S3, carrying out an enzymatic reaction in the first solution system obtained in step S2 under water bath conditions; S4, after the enzymatic reaction is completed, obtaining a second solution system, subjecting the second solution system to enzyme inactivation treatment, and obtaining a de-astringent olive product after cooling and centrifugation; wherein the color-protecting agent is citric acid; the de-astringency rate of the de-astringent olive product is >62.0%, and the browning degree is ≤0.219.

This invention discloses a closed-loop antibacterial cat litter and method for the gradient decomposition of tea polyphenols by activated zeolite with a seven-enzyme gradient. The raw material composition, by weight of the cat litter, includes: 40-60 parts activated zeolite, 20-35 parts corn starch, 0.5-2 parts compound bio-enzyme, 0.3-1.5 parts tea polyphenols, 1-5 parts binder, and 0.1-0.5 parts retention aid. The compound bio-enzyme is a seven-enzyme gradient combination, including: 15-25% ammonia oxidase, 15-25% glutamate dehydrogenase, 10-20% laccase, 10-20% protease, 8-15% lipase, 8-15% amylase, and 6-7% cellulase. The preparation process employs low-temperature hydraulic dry granulation technology. This invention solves the problems of insufficient odor control, easy loss of bio-enzyme activity, and poor antibacterial effect in existing cat litter products. The resulting cat litter has high adsorption capacity, long-lasting and stable deodorization, and significant antibacterial effect. The preparation process is environmentally friendly and efficient, and can be widely used in the pet cleaning field.

This invention discloses a highly efficient strain of *Bacillus tekirii* BB that degrades PLA microplastics and its applications. This strain was deposited on February 2, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing, with accession number CGMCC NO. 37630. This strain can secrete multiple enzyme systems, including lipase, amylase, laccase, and catalase. In soil systems, this strain can effectively degrade PLA, increase soil lactic acid content and the activity of various soil enzymes, and improve soil organic matter, nitrogen, phosphorus, and potassium nutrient status. This strain can alleviate the growth stress of PLA pollution on plants, increasing the plant height, biomass, and antioxidant enzyme activity of crops such as buckwheat. This strain combines PLA degradation, soil remediation, and plant growth promotion functions, making it suitable for in-situ bioremediation of PLA microplastic pollution in farmland and possessing significant environmental and agricultural application value.

The present application is suitable for the field of bioremediation technology, and provides a bioremediation preparation for heavy oil pollution, which comprises an immobilized heavy oil degrading bacterial population preparation, an immobilized laccase preparation, a slow-release nitrogen and phosphorus nutrient preparation, and a rhamnolipid surfactant; the bioremediation preparation is a solid preparation. The present application also provides application of the bioremediation preparation in a heavy oil pollution intertidal zone. The bioremediation preparation has the advantages of not being easy to lose, fast bioremediation starting speed, being superior in competition with indigenous bacterial species, and good environmental tolerance, thereby overcoming the problems of slow starting speed and fast failure speed of traditional bioremediation preparations in remediation of heavy oil pollution intertidal zones.

The application discloses a kind of MSNs@Au-LAC@PDA-PPy composite material and its preparation method and application.It is mainly prepared by MSNs@Au, lacase LAC, polydopamine PDA and polypyrrole PPy composite;Preparation is dissolved in acetic acid-sodium acetate buffer lacase to prepare lacase solution, MSNs@Au is added into lacase solution and is ultrasonic, stirring, soaking and acetic acid-sodium acetate buffer is washed by centrifugation, then hydrochloric acid dopamine, pyrrole and Tris solution, initiator are added, stirring is used at specific temperature Distilled water is washed by centrifugation, finally freeze-drying treatment obtains final MSNs@Au-LAC@PDA-PPy composite material, and further preparation electrode is used for bisphenol A detection.The application prepares high-sensitivity electrochemical sensor, is used for the high-sensitivity detection of BPA in complex matrix, can realize the efficient fixation and protection of lacase.

This invention discloses a material for highly selective detection of alkaline phosphatase, its preparation method, and its applications, belonging to the field of analytical chemistry. The material for highly selective detection of alkaline phosphatase comprises a phosphate-decomposed framework and laccase encapsulated within the framework. The material of this invention eliminates the need for exogenous H2O2 and peroxidase, utilizing framework encapsulation to achieve controlled "encapsulation-release" of laccase, thereby realizing in-situ cascade catalytic amplification. The proposed dual-product (phosphate and phenol) synergistic gating strategy effectively eliminates false positive signals caused by endogenous phosphate or phenolic interferences, providing a universal framework for designing highly selective and sensitive biosensors, and overcoming the fundamental defect of poor accuracy in complex samples in traditional direct coupling methods.

This invention discloses a sampling and observation device and operating method for preliminary judgment of the natural resin formation of agarwood, relating to the field of guiding mechanism technology. It includes a main body, a sampling structure at one end of the main body, a pushing component at the end of the sampling structure away from the main body, and a sampling channel at the axis of the sampling structure. One end of the sampling channel is connected to the main body. The sampling structure includes a sampling component and a gripping part for sampling agarwood. The pushing component includes a pushing part, the outer diameter of which is the same as the inner diameter of the sampling channel. The sampling component allows for minimally invasive sampling of the agarwood tree, avoiding destructive sampling methods such as cutting down trees or drilling holes. Only a small incision needs to be made in the tree to obtain samples from inside the trunk. Furthermore, because agarwood is relatively viscous, the pushing part can push the sample inside the sampling channel into the main body, preventing the agarwood from clogging the sampling channel.

This invention relates to a method for improving the intestinal tolerance of wheat bran dietary fiber based on synergistic modification by microorganisms and enzymes. The method includes ultra-fine grinding and high-pressure moist heat activation of wheat bran; multi-enzyme synergistic microbial stepwise treatment, first using high-temperature amylase and neutral protease to remove interfering components such as starch and protein, then using laccase to degrade lignin, resulting in a looser structure; subsequently, phytase efficiently degrades phytic acid, Aspergillus nidulan degrades the side chains of arabinogalactan, and snail enzyme and ferulic acid esterase work synergistically to convert insoluble arabinoxylan into soluble dietary fiber and release phenolic antioxidants. This method increases the soluble dietary fiber content of wheat bran by 2 times, reduces phytic acid to 10 mg / g, increases free phenolic acid content by 40%, achieves a DPPH free radical scavenging rate of 60%, significantly enhances antioxidant activity, and significantly reduces IgG levels in mouse models compared to those consuming unmodified wheat bran, thus improving intestinal tolerance and probiotic function; the product can be used as a high-quality functional food ingredient.

The application belongs to the field of environmental catalytic materials and wastewater treatment technology, and particularly relates to a zinc-doped graphite carbon nitride material for loading laccase, immobilized laccase and a preparation method and application thereof. The zinc-doped graphite carbon nitride material for loading laccase is prepared by mixing zinc salt, urea and deionized water in a ratio of (0.04g-0.08g):8g:20mL, drying, and calcining under an inert atmosphere to obtain a solid material, and then silanizing the solid material to obtain the zinc-doped graphite carbon nitride material. The immobilized laccase is used to degrade pollutants with a concentration of 10mg / L-30mg / L and a pH of 2-11.

The application discloses a laccase mutant, and preparation and application thereof, and solves the technical problem of providing an enzyme mutant with higher enzyme activity and a complex formed by the enzyme mutant and bacillus subtilis with higher enzyme stability. The application specifically discloses a protein, which is any one of the following: B1) a protein with an amino acid sequence shown in sequence 3; B2) a protein with more than 80% identity with the protein shown in B1) and with the same function, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein in B1); and B3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the protein in B1) or B2). The protein can be used alone to degrade zearalenone and aflatoxin B1, or the protein can be mixed with bacillus subtilis to form a complex, so that the stability of the enzyme can be improved, and the complex can be used in industrial production.

The application discloses a kind of peroxidase / laccase-like nanoscale enzyme colorimetric / fluorescence dual-mode rapid detection sodium dehydroacetate method, the method with 1-hydroxybenzotriazole (HOBT) as organic ligand, select Cu and Zn two transition metals as central ion, Cu / Zn-MOF material is synthesized.The linear range of colorimetric mode is 0.067-12 μg / mL, and the detection limit is 0.020 μg / mL;The linear range of fluorescence mode is 0.01-22 μg / mL, and the detection limit is 0.041 μg / mL, the sensor is applied to the recovery experiment of DHA in food sample, the recovery rate is between 92.1%~106.4%, and RSD is not more than 5%, verify that the analysis method has good stability, reliability and reproducibility.

The application belongs to the field of food safety detection, and relates to a kind of multi-pesticide residue detection method based on Cu@MOF-808-NH2 nanoenzyme sensing array.The application first synthesizes Cu@MOF-808-NH2 nanoenzyme with phosphatase-like, laccase-like, peroxidase-like activity and fluorescence characteristics by hydrothermal method; then, the optimal reaction conditions of each enzymatic activity are systematically optimized, and a four-channel sensor array detection system is constructed; the application also comprehensively utilizes PCA method and HCA method to reduce the dimension of multi-dimensional response data, realizes cross-validation and qualitative identification of different types of pesticides through visual clustering and tree diagram analysis. Further, by analyzing the quantitative relationship between the response signal value of a specific channel and the pesticide concentration, a linear regression equation is constructed, and the precise quantification of the specific pesticide residue concentration in the sample under test is realized, which has the advantages of simple operation, good stability, strong anti-interference ability, wide applicability and the like.

This invention provides laccase mutants and their applications. The laccase mutants, relative to the amino acid sequence of the wild-type laccase shown in SEQ ID NO:1, have one or more of the following mutations: M40K, D50N, F68L, N83T, D206A, I226V, and V268T. The laccase mutants of this invention exhibit significantly enhanced enzyme activity at 0–45°C, with peak activities at 0°C, 15°C, 30°C, and 45°C, respectively, being 3.88 times, 5.24 times, 6.47 times, and 6.42 times that of the wild-type. They also exhibit significantly enhanced enzyme activity at pH 3.0 and pH 4.0, with peak activities at pH 3.0 and pH 4.0, respectively, being 7.02 times and 6.97 times that of the wild-type. In exemplary industrial applications for the degradation of crystal violet and lignin, they have demonstrated excellent degradation capabilities, laying a solid foundation for the application of laccase.

This invention relates to the field of preservation films, specifically to a nanocomposite film for apple storage and preservation and its preparation method, comprising a base film and a coating liquid. This invention combines a cellulose nanocrystal base film with an evaporation-induced self-assembled layered ordered structure with a superhydrophobic cellulose ester coating. A cyclodextrin-laccase cascade system is integrated in the base film to specifically capture and enzymatically degrade the pathogenic factors of tiger skin disease. A micro-nano rough structure is constructed in the coating to encapsulate humidity-triggered microencapsulated degradation enzymes. Compared with existing technologies, this invention simultaneously achieves multiple effects on a single film: passive regulation of the respiratory microenvironment, active removal of stored pathogens, surface anti-condensation, and accelerated degradation on demand after disposal. It leaves no harmful residues throughout its entire life cycle and has broad application prospects in the cold chain logistics and green packaging of fresh agricultural products.

The application provides a method for pork head texture restructuring based on complex enzyme treatment, comprising the following steps: dissolving transglutaminase, laccase, soybean protein isolate and epigallocatechin gallate in a curing liquid, mixing the curing liquid with pork head in a vacuum tumbling machine for tumbling, performing press molding, and performing gradient temperature cooking. The application combines the meat product processing technology, uses the combined use of transglutaminase and laccase to promote the texture restructuring and bonding process of pork head meat in the press molding and product cooking process to a greater extent, and enhances the catalytic effect of transglutaminase and laccase through the addition of soybean protein isolate and epigallocatechin gallate and the vacuum tumbling and programmed temperature rising process. Finally, the structure characteristics (texture characteristics, meat color, microstructure) and the generation amount of flavor substances of the product are improved, and the final product has better overall acceptability. The method is simple and easy to implement, and has a broad application prospect.

This invention discloses a bacterial residue compound fermentation agent and its preparation method, belonging to the field of biotechnology. The fermentation agent is composed of *Bacillus subtilis*, *Lactobacillus plantarum*, and *Candida utilis* in a specific live bacteria ratio, and is synergistically formulated with cellulase, xylanase, laccase, and peroxidase. The total bacterial dry matter accounts for 35%–45%, and the compound enzyme system accounts for 55%–65%. The preparation method includes: step S1, bacterial culture and powder preparation; step S2, bacterial powder mixing and compounding; step S3, enzyme powder preparation; and step S4, mixing and sieving. This invention achieves efficient degradation of lignocellulose, enrichment of crude protein, and efficient chemical degradation of aflatoxin B1, zearalenone, and vomitoxin through dynamic pH control and metabolic flux distribution. When used for corn cob treatment, this fermentation agent significantly improves the production performance, nutrient digestibility, and antioxidant and immune functions of beef cattle.

This invention discloses a composite microbial preparation for solid fecal degradation, its preparation method, and its application, belonging to the field of microbial preparation technology. The composite microbial preparation comprises a composite microbial agent and an exogenous composite enzyme preparation; the composite microbial agent includes laccase-producing bacteria, keratinase-producing bacteria, auxiliary degradation bacteria, and composite probiotics; the exogenous composite enzyme preparation includes cellulase, neutral protease, and xylanase. This invention employs a mixed strategy of "exogenous enzyme + enzyme-producing bacteria," rapidly decomposing cellulose, protein, and hemicellulose in the initial stage of composting by adding exogenous cellulase, protease, and xylanase, providing readily available carbon and nitrogen sources for the microorganisms, shortening the composting start-up time to less than 24 hours; simultaneously, the laccase-producing bacteria and keratinase-producing bacteria continuously produce laccase and keratinase during composting, achieving deep degradation of lignin and keratin. This invention has advantages such as rapid start-up, continuous degradation, good deodorization effect, high nitrogen retention rate, and low cost, and has good prospects for industrial application.

This invention belongs to the field of pesticide formulation and discloses a UV-resistant and laccase-responsive nanopesticide slow-release agent and its preparation method. The UV-resistant and laccase-responsive nanopesticide slow-release agent is a composite nanoparticle formed by self-assembly of a fungicide active ingredient and a phthalate-modified Pluronic F127 and lignin sulfonate as carriers; the fungicide active ingredient is a photodegradable small-molecule pesticide fungicide. This invention's nanopesticide controlled-release agent can effectively increase the water solubility and liquid surface adhesion efficiency of pesticides, while also exhibiting specific enzyme responsiveness, releasing the drug only within fungi where laccase is present, and having minimal toxic side effects on humans and aquatic organisms such as fish that do not contain laccase.

The application discloses a preparation method of a copper-iron bimetallic nano-enzyme and application thereof, and belongs to the technical field of nano-enzyme preparation and catalytic detection, and solves the technical problems of single nano-enzyme activity, low detection flux, and difficulty in simultaneously detecting multi-component pesticide residues in the prior art. The GMP, copper ions and iron ions are subjected to self-assembly reaction in a buffer solution, and after separation and drying, a GMP-CuFe bimetallic nano-enzyme is obtained. The GMP-CuFe nano-enzyme has dual activities of peroxidase and laccase, a colorimetric array sensor comprising three sensing channels of LAC incubation, LAC and POD is constructed, and by utilizing the differential colorimetric signals generated after different pesticides and the nano-enzyme act in different channels, and by combining linear discriminant analysis, the glyphosate, nicosulfuron, halosulfuron-methyl, metsulfuron-methyl and ferrous methylene bis (dimethyl dithiocarbamate) can be distinguished and quantitatively detected. The preparation method is simple and low in cost, the sensor array is high in sensitivity, good in selectivity and strong in anti-interference capability.

The technical problem of this invention lies in providing a novel protein cross-linking method. This method promotes the cross-linking reaction by allowing both oxidoreductases such as laccase and protein deamidases such as protein glutaminase to act on the substrate protein.

The invention relates to the technical field of biology. The invention provides a strain of Neurospora crassa N7, and the preservation number of the Neurospora crassa N7 is CGMCC (China General Microbiological Culture Collection Center) No.40738. The Neurospora crassa N7 can be used for preparing a microbial inoculum. The strain is preserved in the China General Microbiological Culture Collection Center on July 24, 2023, the China General Microbiological Culture Collection Center is called CGMCC for short, and the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Neurospora crassa N7 provided by the invention can directly utilize straws to produce cellulase, hemicellulase and laccase, has a good effect in the aspect of decomposing or degrading the straws, not only can effectively degrade straw lignocellulose under a liquid culture condition, but also can effectively degrade the straw lignocellulose in a soil environment. In addition, the strain has wide temperature adaptability, and has a good effect on promoting straw in-situ decomposition in normal-temperature and low-temperature environments.

The invention relates to the technical field of straw decomposition, and discloses a complex microbial inoculant for straw decomposition as well as a preparation method and application of the complex microbial inoculant. Comprising the following components in parts by mass: 10-20 parts of bacillus subtilis, 20-30 parts of aspergillus niger, 15-25 parts of penicillium oxalicum, 10-14 parts of cellulase, 6-10 parts of xylanase, 3-8 parts of laccase, 1-5 parts of a first degradation carrier, 5-10 parts of a second degradation carrier, 3-8 parts of a composite freeze-drying protective agent, 0.5-1.5 parts of a pH buffer agent and 0.3-0.8 part of an enzyme activity protective agent, according to the preparation method disclosed by the invention, the viable count of the complex microbial inoculant is high, and an exogenous enzyme is added, so that the decomposition period of the straws is greatly shortened.

This invention discloses a gene, TaLAC58, capable of enhancing wheat resistance to Fusarium head blight and its applications. The TaLAC58 gene belongs to the laccase family and is a gene specifically expressed in the resistant wheat variety Sumai 3. This gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in stable transgenic plants. In the three transgenic lines obtained, resistance to Fusarium head blight was significantly enhanced. This invention has significant production implications for improving wheat resistance to Fusarium head blight and provides a new gene resource for breeding high-yielding, stable-yielding, and widely adaptable new wheat varieties.

This invention relates to the field of predicting minor water loss in highland barley plants, specifically to a method for predicting minor water loss in highland barley plants based on DETR multimodal fusion. The technical solution includes: sampling minor water loss in highland barley plants and acquiring multimodal data to obtain an original dataset containing flag leaf hyperspectral data, whole-plant RGB data, and true water content labels; preprocessing the flag leaf hyperspectral data and RGB image data in the original dataset; extracting one-dimensional spectral deep features related to water content within the flag leaf and two-dimensional visual deep features related to water stress in the whole-plant canopy; fusing multimodal features based on a DETR encoder to output multimodal global features; constructing an improved DETR regression model; training the improved DETR regression model using the original dataset; and using the trained model to predict minor water loss in highland barley plants. This invention is applicable to predicting minor water loss in highland barley plants.

This invention discloses a highly efficient and harmless composting method for livestock and poultry manure based on enzymatic pretreatment, belonging to the field of livestock and poultry manure composting technology. It includes two stages: enzymatic pretreatment and dynamic control of composting. The specific steps are as follows: Enzymatic pretreatment: Livestock and poultry manure is mixed with auxiliary materials at a mass ratio of 1:0.5-1:0.8, and the moisture content is adjusted to 55%-65%. A compound enzyme preparation is added, comprising cellulase, xylanase, laccase, and neutral protease, with an enzyme activity ratio ranging from 3:2:1:1.5. This invention significantly improves composting efficiency and material degradation rate through the enzymatic pretreatment stage: Pretreatment of livestock and poultry manure with a compound enzyme preparation containing cellulase, xylanase, laccase, and neutral protease effectively degrades complex organic matter such as cellulose, hemicellulose, lignin, and proteins, achieving a relative cellulose degradation rate of 25%-40%, laying a good foundation for the subsequent composting process and significantly shortening the composting cycle.

This invention discloses an amylase-co-laccase-modified millet straw adsorbent material, its preparation method, and its application, belonging to the technical field of adsorbent materials. The preparation steps of the amylase-co-laccase-modified millet straw adsorbent material include: soaking cooked millet straw in an amylase solution, removing it, soaking it in a laccase solution, then inactivating the enzyme, washing, and drying to obtain the amylase-co-laccase-modified millet straw adsorbent material. The raw material used in this invention—millet straw—is an agricultural waste resource, abundant in resources and rich in active groups such as cellulose and lignin, possessing excellent adsorption potential. This invention uses millet straw as a raw material to develop a highly efficient bioadsorbent for nicotine adsorption through modification and other technologies, and prepares a high-adsorption-capacity millet straw adsorbent material through optimized preparation processes. This is beneficial for protecting human health and also features low energy consumption and green, efficient utilization of millet straw agricultural and forestry waste.

This invention relates to a method for preparing an antioxidant coupling compound based on peach peel residue polyphenols, comprising the following steps: (1) steam explosion treatment, drying, and ultrafine pulverization of peach peel residue; (2) compound enzymatic hydrolysis of the ultrafine pulverized peach peel residue: solid-liquid separation of the hydrolyzed material, and collection of the supernatant; (3) adsorption of the supernatant through a macroporous adsorption resin, followed by elution, concentration, and drying to obtain an antioxidant functional raw material rich in peach polyphenols; (4) mixing peach polyphenols with ascorbate palmitate, adding laccase for catalysis to form an ascorbate palmitate-peach polyphenol coupling compound. Applying this coupling compound to fish oil can effectively extend the shelf life of fish oil and inhibit the oxidative decomposition of DHA and EPA. This invention generates highly active free radicals through dual-substrate activation, endowing the product with stable antioxidant properties, while simultaneously achieving efficient extraction and resource utilization of polyphenols from peach peel residue, significantly improving the utilization rate and economic value of by-products.

This invention relates to the field of wood adhesives and bioresins, and discloses a large-scale production process for high-adhesion-strength formaldehyde-free bioresin, comprising: pre-treatment of plant phenolic biomass raw materials with wood ash alkali activation to release free phenolic hydroxyl groups; physical adsorption and immobilization of laccase and soybean peroxidase on a bamboo charcoal carrier to obtain a bamboo charcoal dual-enzyme co-immobilized composite; mixing activated phenolic solution with soybean protein isolate solution, adding bamboo charcoal dual-enzyme body and batch-added components to form a phenol-phenol and phenol-protein dual-type covalent cross-linking network; sequentially adding pre-activated dehydrated clinoptilolite and flaxseed gum; coating the obtained adhesive onto a substrate and then hot-pressing and curing, followed by cooling and curing to obtain a dense, microporous-free cured adhesive layer. This invention achieves complete formaldehyde-free adhesive, with adhesive strength reaching the standards for engineering-grade boards, eliminating microporous defects in the hot-pressed adhesive layer, and using raw materials entirely derived from nature or industrial and agricultural by-products, making it suitable for large-scale continuous production.