205 results about "Laccase" patented technology

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention relates to the technical field of nano-enzymes, and discloses a copper-based nano-enzyme with laccase and peroxidase double-enzyme activity and preparation and application thereof, and the copper-based nano-enzyme is a nano-material prepared by forming MOF through solvothermal reaction of copper ions and diamino terephthalic acid and then performing electrostatic adsorption on the MOF and polyoxometallate. The copper-based nano enzyme has high-efficiency laccase-like activity and peroxidase-like activity at the same time through the combination and synthesis process of the copper ion cluster and the organic ligand. The invention further discloses a preparation method of the copper-based nano-enzyme, peroxidase activity detection and application of the copper-based nano-enzyme to degradation and determination of dichlorophenol. The copper-based nano-enzyme provided by the invention has high catalytic activity and stability, is low in cost and easy to prepare, and can be applied to biosensing detection of hydrogen peroxide and environmental remediation degradation of organic pollutants such as phenols and dyes.

This invention discloses a laccase mutant, Lcc5-I479T, its expression strain, and its applications. Using laccase from *Coprinopsis cinerea* as the starting enzyme, this invention obtained the mutant gene through molecular docking and positional analysis. After induction expression in engineered bacteria containing the mutant gene, the stable laccase mutant enzyme Lcc5-I479T was obtained. Using ABTS as a substrate, the mutant enzyme Lcc5-I479T showed a stable increase in activity to 8-20 times that of the starting enzyme at 35-50℃. This mutant enzyme has potential application value in the oxidative transformation of aflatoxin.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

The application discloses a laccase-producing polyporus brumalis and a method for preparing laccase. The application provides polyporus brumalis CCTCC NO: M 2020809 for fermenting and preparing laccase, and the amino acid sequence of the laccase is shown as SEQ ID NO: 2. The fermentation level of the laccase is high, the fermentation level in a flask reaches 980 U / mL, and the fermentation level in a 70L fermenter reaches 880 U / mL. In order to ensure the food safety of the laccase, the strain and the preferred medium components of the laccase are edible. In addition, the laccase obtained by the application can catalyze the polymerization of film-forming monomers to generate a copolymer film, and is a method for synthesizing a copolymer film by using a biological enzyme catalyst.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

The invention discloses a protein-based multifunctional food preservative film as well as a preparation method and application thereof, and belongs to the technical field of functional modification of natural polymer materials. The preparation method of the protein-based multifunctional food preservative film comprises the following steps: firstly, carrying out hydrolysis pretreatment on zein by utilizing the hydrolysis effect of protease, then grafting catechol into a protein structure by utilizing an acyl transfer reaction of TGase, and finally, carrying out functional grafting modification on the zein by utilizing chitosan by utilizing an oxidation reaction of laccase, so as to obtain the protein-based multifunctional food preservative film. The physical and mechanical properties, the stability and the antibacterial activity of the zein film are further improved, and the protein film is endowed with antioxidant activity, so that the protein-based multifunctional food preservative film material suitable for food preservation is prepared. When the protein-based multifunctional food fresh-keeping film is used for fruit fresh-keeping treatment, the shelf life is remarkably prolonged, and the protein-based multifunctional food fresh-keeping film has a good fresh-keeping effect.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention discloses iron-based nano laccase and a preparation method and decoloration application thereof, and relates to the technical field of iron-based nano laccase preparation, and the preparation method comprises the following steps: S1, iron-based nano carrier pretreatment; s2, synergistically modifying the iron-based nano-carrier; s3, preparing a laccase buffer solution; s4, carrying out ultrasonic and microwave synergistic in-situ loading; s5, step-by-step curing is carried out; s6, gradient purification; and S7, vacuum drying. According to the method, the binding effect of the laccase and the carrier is greatly improved, the problems of low laccase loading rate and large enzyme activity loss in the prior art are effectively improved, the product has excellent catalytic activity and stability, meanwhile, the binding firmness of the laccase and the carrier is further enhanced through the step-by-step curing process, laccase falling in the using process is reduced, and the service life of the product is prolonged. And in combination with excellent magnetic responsiveness of the iron-based nano-carrier, rapid separation, recovery and reutilization of the product are realized, and the practical application cost is greatly reduced.

This invention discloses a laccase gene PmLAC31 derived from *Pinus massoniana* and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the *PmLAC31* laccase gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. This invention also provides biomaterials containing the gene, and the application of this gene or biomaterials in improving plant drought resistance and / or lignin content. Experiments show that heterologous overexpression of the PmLAC31 gene in poplar significantly promotes lignin deposition and enhances the plant's drought tolerance. This invention provides important gene resources and application pathways for molecular breeding of forest trees for stress resistance.

The invention belongs to the field of polishing solutions, and particularly relates to a green polishing solution based on an enzymatic Fenton system. The 4H-SiC substrate polishing solution comprises a component A and a component B, the effective component of the component A is an enzyme-catalyst co-immobilized abrasive material, and the enzyme-catalyst co-immobilized abrasive material comprises an abrasive material matrix, laccase and an iron-based catalyst, and the laccase and the iron-based catalyst are loaded on the abrasive material matrix; the active ingredient of the component A is a phenol substrate, the active ingredient of the component B is a phenol substrate, after the component A and the component B are mixed, laccase catalyzes oxidation of the phenol substrate to generate H2O2 in situ, the H2O2 and an iron-based catalyst are subjected to a Fenton reaction, and hydroxyl free radicals are generated at a polishing interface in situ. According to the invention, continuous, efficient and stable supply of hydroxyl radicals is ensured through enzymatic endogenous hydrogen peroxide circulation and catalyst regeneration circulation, so that the polishing solution can realize long-time stable polishing under a nearly neutral condition, and has the advantages of environmental protection, high polishing efficiency, good surface quality and the like.

The application relates to the technical field of sponge production, and discloses a production process of high-density elastic environment-friendly sponge. The application realizes gradient foaming under laminar flow control by designing pH-sensitive three-layer core-shell laccase microcapsules with a particle size of 3-4 microns and an enzyme activity of 180-220 U / g, combining a bio-based polyester matrix with a water and liquid CO2 composite foaming system; the process core comprises the following steps: enzyme triggering solidification, microcapsule rupture triggered by pressure relief at 55-65 DEG C, crosslinking completed by three-stage gradient heating at 75-85 DEG C; a double-gradient structure with a surface layer bubble hole density of 150-190 cells / cm3 and a core layer bubble hole density of 75-95 cells / cm3 is formed; finally, the density is 90-110 kg / m3, the compression permanent deformation is less than or equal to 5%, VOCs is less than or equal to 0.02 mu g / m3, and the biodegradation rate is greater than 90%; the process solves the problems of poor durability and insufficient environmental protection of traditional sponges.

The present application belongs to the technical field of biological remediation and solid organic waste treatment and resource, and particularly relates to application of phenolic compounds directly degradable by laccase in compost and composting method for promoting humification. The present application uses phenolic compounds directly degradable by laccase in compost, and the composting process incubates a large number of microbial communities. When nutrients are sufficient, most of the microorganisms will show competitive response; and under stress environment, the microorganisms tend to be synergistic. In the early stage of the composting reaction, the humification process in the compost is often slow, and the microorganisms will make full use of the humic acid precursor, slowing down the composting. The toxicity of the phenolic compounds directly degradable by laccase can promote the synergistic function of the microorganisms in the compost, and because it can enhance the expression of laccase, it can promote the co-metabolic effect of laccase on pollutants and lignin, degrade the pollutants while promoting the humification of the compost, and protect the environment.

The application belongs to the technical field of bioengineering, and particularly relates to a high-temperature-resistant laccase mutant obtained by site-directed mutation through an overlapping PCR technique, and preparation and application thereof. The laccase mutant is obtained on the basis of a wild-type laccase shown in SEQ ID NO. 1, and at least one of D221Y, E231D, Y441H or Y441F mutations occurs, and the mutant enzyme activity and the thermal stability at 80 DEG C are both improved to different degrees relative to the wild type, which is beneficial to expand the application in the industrial fields of degrading lignocellulose, paper pulp bleaching and the like.

The invention discloses a composite leavening agent and application of the composite leavening agent in high epiphytic microorganism and high lignocellulose straw silage and biomass pretreatment. The technical problem to be solved is how to improve the quality of silage obtained by fermenting silage raw materials (especially silage raw materials with high epiphytic microorganisms and / or high lignocellulose) and / or shorten the fermentation time of the silage and / or improve the cellulose conversion rate of the silage raw materials. The composite leavening agent provided by the invention is prepared from lactobacillus casei TD, lactobacillus plantarum JT-10, laccase and cellulase. The method can be used for fermentation of silage raw materials with high epiphytic microorganisms and high lignocellulose, the quality, nutritional value and the like of silage can be effectively improved, the conversion efficiency of biomass cellulose can be improved, and biological energy can be produced through pretreatment.

The invention discloses a fermentation method for fungus-enzyme synergistic treatment of lucid ganoderma and application of the method in pet food. The fermentation method comprises the following steps: (1) enzymolysis pretreatment: mixing ganoderma lucidum sporocarp powder with water, adding cellulase and laccase, and performing enzymolysis to obtain an enzymolysis solution; (2) sterilizing and supplementing a carbon source: adding the carbon source into the enzymatic hydrolysate, and sterilizing; (3) lactobacillus fermentation: adding lactobacillus plantarum into the sterilized enzymatic hydrolysate, and fermenting under a fermentation condition to obtain fermentation liquor; and (4) solid-liquid separation: performing centrifugal separation on the fermentation liquor, and collecting supernate to obtain the ganoderma lucidum fermentation liquor. According to the method, the laccase and the cellulase are synergistically broken, so that the release efficiency of triterpenes and total phenols in the ganoderma lucidum with spores is remarkably improved. The fermentation liquor can improve lipid accumulation (the oil red O staining lipid content is reduced) and relieve oxidative stress injury on the cell level, and can be used for preparing functional food for improving metabolic health, especially for lipid abnormality and oxidative stress state related to metabolic dysfunction of pets.

To achieve the unique aesthetic appeal of lacquer while providing a mycelial structure with excellent strength, water resistance, and abrasion resistance. [Solution] The mycelial structure 100 of the present invention comprises mycelium and lacquer that has permeated the mycelium. Preferably, the mycelium contains laccase enzyme. Preferably, the mycelial material 10 comprises a base 11 having mycelium and a lacquer-penetrating layer 13 in which the lacquer has permeated the mycelium, and a lacquer coating layer 20 provided on the surface of the lacquer-penetrating layer 13. Preferably, the lacquer coating layer 20 is thicker than the lacquer-penetrating layer 13. Preferably, the thickness of the lacquer coating layer 20 is 5.0 μm or more and 100.0 μm or less. Preferably, the thickness of the lacquer-penetrating layer 13 is greater than 0.0 μm and 30.0 μm or less.

The invention discloses a nutrient signal substance synergistic controlled-release fertilizer for promoting efficient absorption of phosphorus in rice, and belongs to the technical field of controlled-release fertilizer production. Three technical breakthroughs are realized by constructing a'microbial response outer membrane layer-enzyme response inner membrane layer 'dual biological response controlled release system and cooperating with an'organelle targeted phytic acid synthesis signal guide chain substance': firstly, phosphorus release is synchronized with a rhizosphere activity period through the microbial response characteristic of an outer membrane; secondly, accurate release of signal substances in the filling stage is ensured through an inner layer film laccase triggering mechanism; 3, an endogenous signal channel of the plant is activated by utilizing an IP3 analogue precursor molecule, so that the synthesis of phytic acid is enhanced from a metabolism level, and a'grain bank strong 'is created to pull the absorption and transport of phosphorus, thereby systematically improving the utilization efficiency of the phosphate fertilizer and realizing normal form transformation from'passive supply' to'active demand '.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

The application discloses a method for super-hydrophilic modification of polyamide fiber based on DES combined with multiple enzymes, and belongs to the technical field of functionalized textiles.The method for super-hydrophilic modification specifically comprises the following steps: immersing the polyamide fabric after refining treatment into DES for pretreatment, taking out and drying; then immersing into a protease modification solution for reaction; after the reaction is completed, washing, drying and balancing; then immersing into a mixed solution formed by laccase, TEMPO, trehalose and a buffer solution for reaction; after the reaction is completed, washing, drying, balancing and hydrophilic modification of the polyamide fabric are obtained; the super-hydrophilic modified polyamide fabric can reach a surface static contact angle of 9 degrees within 60 seconds, and the contact angle is reduced by 90.2 percent; the method has low energy consumption, is safe and environmentally friendly, and has good durability of hydrophilic modification.

The invention relates to the technical field of reverse osmosis membranes, and discloses a high chlorine-resistant hydrophilic membrane and a preparation process thereof.The hydrophilic membrane comprises a membrane body, a first chlorine-resistant layer and a second chlorine-resistant layer, the first chlorine-resistant layer and the second chlorine-resistant layer are sequentially arranged on the surface of the membrane body, the first chlorine-resistant layer is prepared from laccase, dopamine, 5, 5-dimethylethylhydantoin and a stabilizer, the laccase concentration is 0.1-0.3 wt%, the dopamine concentration is 0.1-0.3 wt%, and the stabilizer concentration is 0.1-0.3 wt%. The concentration of the dopamine ranges from 0.8 wt% to 1.2 wt%, the concentration of the 5, 5-dimethylethyl hydantoin ranges from 0.3 wt% to 0.6 wt%, and the concentration of the stabilizer ranges from 0.2 wt% to 0.4 wt%; the second chlorine-resistant layer is obtained by crosslinking first carboxymethyl dextran and second carboxymethyl dextran, the molecular weight of the first carboxymethyl dextran is smaller than that of the second carboxymethyl dextran, and the weight ratio of the first carboxymethyl dextran to the second carboxymethyl dextran is (1-5): 1. According to the invention, the durability of chlorine resistance can be improved, and good water flux can be maintained.

The invention relates to the field of pulping and papermaking, in particular to a mediator-free laccase delignification bamboo pulp bleaching method which comprises the following steps: S1, mixing laccase and absolute dry pulp, adjusting pulp concentration, introducing oxygen at a certain temperature for reaction, washing with deionized water, spin-drying and scattering; the absolutely dry pulp is obtained by defibering, screening and drying unbleached chemical bamboo pulp. And S2, carrying out acid pickling chelation on the absolutely dry slurry treated in the step S1. And S3, bleaching the absolutely dry slurry treated in the step S2. According to the method, the lignin in the bamboo pulp is efficiently removed, the kappa number is reduced by more than 40%, the biological enzyme is directly used for replacing about 30% of total chemicals under the condition of not changing the section of an oxygen removal tower in the original process, and the consumption of chemicals and the discharge of bleaching wastewater in industrial production are reduced; and meanwhile, no expensive mediator is added, so that the problem of corrosion of the mediator to equipment is avoided, and the maintenance cost is greatly reduced.

This invention belongs to the field of agricultural fertilizer technology, specifically relating to an organic water-soluble fertilizer based on molasses fermentation concentrate and its preparation method. The organic water-soluble fertilizer comprises molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. It employs a staged temperature-varying enzymatic hydrolysis process. Laccase first oxidizes and degrades polyphenols at 30–35°C, opening the polyphenol-polysaccharide complex structure. Then, the temperature is raised to 42–48°C, where β-glucanase, xylanase, and mannanase directionally degrade colloidal polysaccharides. Simultaneously, EDTA-2Na is introduced to chelate metal ions, and polyaspartic acid inhibits scale dispersion. These three components synergistically form an "enzymatic hydrolysis-chelation-scale inhibition" anti-clogging system. This invention addresses the technical problems of existing molasses fermentation concentrate fertilizers being prone to clogging in drip irrigation systems, having low efficiency in constant-temperature synchronous enzymatic hydrolysis, and having an imperfect single anti-clogging mechanism. It provides an organic water-soluble fertilizer composed of molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. This invention effectively solves the problem of clogging of honey fermentation concentrate in drip irrigation systems, improves enzymatic hydrolysis efficiency, and is suitable for drip irrigation fertilization of various crops such as bananas and vegetables.

The invention discloses a method for improving the quality of rye bread by using a laccase and alpha-amylase composite biological enzyme preparation, and belongs to the technical field of food processing. The method comprises the following steps: adding laccase and alpha-amylase into rye bread raw materials, kneading, carrying out primary fermentation, cutting, shaping, carrying out secondary fermentation, baking and cooling to obtain the rye bread. The laccase-alpha-amylase composite starch has the beneficial effects that laccase and alpha-amylase generate a synergistic effect of '1 + 1gt; 2' on construction of a gluten network, optimization of a starch structure and water distribution through compounding in a specific proportion. The specific volume, the texture and the aging resistance of the rye bread are improved through the comprehensive quality.

The invention relates to the technical field of pet food, in particular to an eye-benefiting and liver-protecting fermentation type canine nutrient and a preparation method thereof, and the eye-benefiting and liver-protecting fermentation type canine nutrient comprises the following components: a liver-protecting component, an eye-benefiting component, a chitosan-calcium compound solution, beer yeast powder, honey, lactobacillus plantarum freeze-dried powder and bacillus subtilis freeze-dried powder. Porous starch of the liver protection component carries and protects N-acetylcysteine, and carnosine and quercetin modified by zein remove fermentation byproducts. The eye benefiting component is loaded and protected by octenyl succinic acid modified starch, and the dispersity and the stability of the xanthophyll are improved by grafting a hydrophobic cavity of sulfobutyl-beta-cyclodextrin. The laccase fixed on the surface of the porous starch catalytically oxidizes phenolic hydroxyl groups in a tannic acid-gelatin network on the surface of the eye benefiting component by using dissolved oxygen, a protective shell is formed by in-situ crosslinking and curing, and a chitosan-calcium compound solution closes the physical distance between the laccase and the tannic acid-gelatin network, so that the enzymatic oxidation reaction rate is increased.

The invention relates to an antibacterial detoxification water solvent and a preparation method thereof, and belongs to the technical field of antibacterial detoxification materials. A formula of the antibacterial and detoxifying aqueous solution comprises the following components in parts by weight: 0.1 to 0.3 part of a zinc-based compound, 0.3 to 0.5 part of microencapsulated laccase, 1 to 3 parts of an organic acid stabilizer, 0.5 to 1 part of cocamidopropyl betaine and 70 to 90 parts of deionized water. Wherein the zinc-based compound is obtained by growing zinc oxide on the surface of metal zinc powder in situ and then forming a silicon dioxide thin layer on the surface. The antibacterial and detoxicating aqueous solvent prepared by the invention has excellent antibacterial and detoxicating effects.

This invention relates to the field of food processing, and provides an enzyme-mediated enzymatic treatment method for olive de-astringency and polyphenol conversion, solving the problems of high pollution from chemical methods and severe browning from traditional enzymatic methods in existing olive de-astringency technologies, resulting in poor product appearance, strong astringent taste, and loss of nutritional active ingredients. The method includes the following steps: S1, preparing an olive raw material slurry; S2, adding laccase to the olive raw material slurry, adding a citrate-sodium citrate buffer solution to adjust the reaction environment, and then adding a color-protecting agent to obtain a first solution system; S3, carrying out an enzymatic reaction in the first solution system obtained in step S2 under water bath conditions; S4, after the enzymatic reaction is completed, obtaining a second solution system, subjecting the second solution system to enzyme inactivation treatment, and obtaining a de-astringent olive product after cooling and centrifugation; wherein the color-protecting agent is citric acid; the de-astringency rate of the de-astringent olive product is >62.0%, and the browning degree is ≤0.219.

Various aspects of this disclosure relate to the discovery that phosphatase and laccase enzymes confound the extraction and quantification of psilocybin and other tryptamines during the preparation of psilocybin-containing products from mushrooms. Various aspects of this disclosure relate to the discovery that ionization states of phosphoryloxytryptamines affect the extraction and quantification of psilocybin and other tryptamine. This disclosure describes methods to denature enzymes, optimize ionization states, and control for other variables to improve extraction yields of tryptamines from mushrooms, manufacturing intermediates, and tryptamine-containing products and to improve the accuracy of methods to quantify tryptamines.

The present application relates to the field of biological enzyme catalyst, especially to a kind of immobilized laccase based on inorganic porous material and its preparation method and application, the immobilized laccase based on inorganic porous material designed in the present application, the immobilized laccase includes inorganic porous material and laccase adsorbed and fixed on the surface of inorganic porous material, the inorganic porous material is prepared by dynamic hydrothermal synthesis method with piperidine as template agent, with aluminum isopropylate as aluminum source, boric acid as boron source, fumed silica as silicon source. Laccase is adsorbed and immobilized by using the inorganic porous material, and the immobilized laccase catalyst obtained has good enzymatic performance, and the immobilized laccase has significant effect in removing quinolone antibiotics.

This invention belongs to the field of microbial inoculant technology and discloses a corn stalk decomposing agent product and its preparation method. The decomposing agent is composed of microbial inoculants, enzyme preparations, nutrient adjuvants, and a modified carrier in specific weight proportions. The microbial inoculants are a compound of Bacillus subtilis, Trichoderma viride, and Aspergillus niger; the enzyme preparation is a complex of cellulase, xylanase, and laccase; the nutrient adjuvant is a mixture of urea, sucrose, and potassium dihydrogen phosphate; and the carrier is an alkali-modified complex of wheat bran and straw powder. The preparation method includes preparing each microbial inoculant separately, alkali-modifying the carrier, and mixing all components. This invention also discloses the application method of this decomposing agent in corn stalk decomposition. This product achieves rapid, efficient, and thorough decomposition of corn stalks through the synergistic effect of multiple components, and the preparation process is stable, the product has a long shelf life, and it is suitable for large-scale production and field application.