310 results about "Laccase" patented technology

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The present invention provides a azelastine hydrochloride nasal spray and a preparation method thereof, belonging to the technical field of nasal sprays. The azelastine hydrochloride nasal spray is composed of the following components by mass percentage: 0.1% of azelastine hydrochloride, 0.3 - 0.4% of a composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower, 0.005 - 0.01% of a stabilizer, 0.002 - 0.01% of a preservative, 0.95 - 1.05% of a thickening agent, 0.7 - 0.8% of a pH regulator, 0.45 - 0.48% of sodium chloride, and the balance of purified water; the composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower is prepared by first subjecting a mixture of chamomile, houttuynia cordata, and peony flower to water extraction to obtain a water extract and then subjecting it to composite enzyme fermentation. The composite enzyme includes cellulase, pectate lyase, and laccase. The present invention can effectively reduce the irritation to the eyes during use and ensure safer and more reassuring use.

The application discloses a kind of fungal laccase mutant Lcc5-G and its expression strain and application.The application is based on the fungal laccase from Coprinopsis cinerea heterologous expression since, by site-directed mutagenesis, obtain mutant gene.After fungal induction expression containing mutant plasmid, obtain laccase mutant enzyme Lcc5-G with improved specific activity, stability and AFB1 removal efficiency.When ABTS is used as substrate, the specific activity of the mutant is improved by 3 times, and the stability of the mutant enzyme is improved to 5 times of the original enzyme at 45 DEG C and pH 7.The mutant has potential application value in oxidizing aflatoxin.

The invention relates to the technical field of sewage treatment, and particularly discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The invention discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The high-suspended-matter sewage treatment agent comprises 40-60 parts of modified bentonite; 5-15 parts of ferrous sulfate; 5-15 parts of aluminum ferric sulfate; 1 to 5 parts of ethylenediamine tetraacetic acid; 10 to 20 parts of an active biochar material; 3 to 8 parts of chitosan; 5 to 10 parts of a flocculating agent; the modified bentonite is obtained by modifying bentonite through laccase and nano zero-valent iron; the active biochar material is obtained by taking waste biomass as a raw material and carrying out semi-carbonization and activation treatment. The sewage treatment agent disclosed by the invention can be used for purifying high-suspended-matter sewage generated by oil extraction plants and oil wells, has high removal efficiency on suspended matters, and can effectively remove organic humus, so that the content of the suspended matters in the treated sewage reaches the standard.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The present disclosure provides a porous microcarrier loaded with laccase, a preparation method and an application. The porous microcarrier loaded with laccase includes: a hydrogel porous microcarrier; laccase, which is loaded on the hydrogel porous microcarrier by covalent bonding; wherein the hydrogel porous microcarrier is a methacrylated hydrogel microcarrier filled with chitosan, providing a loading site for laccase.

The embodiment of the invention provides a fluorescent probe, an application method thereof and a fluorescent probe kit. According to the fluorescent probe provided by the embodiment of the invention, the laccase has relatively high sensitivity and specificity, and the laccase can be accurately detected under the condition that the laccase concentration is relatively low, so that the laccase detection accuracy is effectively improved. When the fluorescent probe provided by the embodiment of the invention is used for laccase detection, the detection cost can be reduced to 1 yuan per time, and compared with the existing ABTS colorimetric method, the cost advantage is better.

The invention relates to high-strength degradable self-healing hydrogel and a preparation method thereof, and belongs to the technical field of high polymer materials. The hydrogel is prepared from the following components in percentage by mass: 15 to 20 percent of carboxymethyl cellulose, 10 to 14 percent of acrylic acid, 15 to 20 percent of N-isopropylacrylamide, 4 to 8 percent of tannic acid, 2 to 4 percent of borax, 0.06 to 0.1 percent of laccase, 0.5 to 1 percent of N, N-methylene bisacrylamide, 0.4 to 0.6 percent of potassium persulfate and the balance of phosphate buffer solution. According to the hydrogel disclosed by the invention, through dual-network structure design, dynamic covalent bond crosslinking and enzyme catalysis green synthesis, collaborative optimization of high strength, high self-healing property, degradability and multi-responsiveness is realized, and the problems of poor mechanical property, low repairing efficiency, environmental unfriendliness and the like of traditional hydrogel are solved; the method has wide application potential in the fields of biomedicine, flexible electronics, intelligent packaging and the like.

The present invention belongs to the field of biodegradation of mycotoxins, and specifically relates to a complex enzyme preparation for degrading aflatoxins, a complex enzyme preparation solution, and its preparation method and application. The complex enzyme preparation comprises: 35-45 wt% tyrosinase, 20-30 wt% cellulase, 8-12 wt% pectinase, 8-12 wt% xylanase, and 10-20 wt% mushroom laccase. Compared with existing chemical and biological detoxification technologies, the complex enzyme preparation of the present invention contains safe and non-toxic components, is simple to use, and has low economic costs. After treatment with the enzyme preparation solution, the cellulose and hemicellulose in the peanut meal are initially decomposed, making it easier for animals to digest and absorb, and having higher nutritional value and better economic benefits.

The invention relates to the technical field of nano-enzymes, and discloses a copper-based nano-enzyme with laccase and peroxidase double-enzyme activity and preparation and application thereof, and the copper-based nano-enzyme is a nano-material prepared by forming MOF through solvothermal reaction of copper ions and diamino terephthalic acid and then performing electrostatic adsorption on the MOF and polyoxometallate. The copper-based nano enzyme has high-efficiency laccase-like activity and peroxidase-like activity at the same time through the combination and synthesis process of the copper ion cluster and the organic ligand. The invention further discloses a preparation method of the copper-based nano-enzyme, peroxidase activity detection and application of the copper-based nano-enzyme to degradation and determination of dichlorophenol. The copper-based nano-enzyme provided by the invention has high catalytic activity and stability, is low in cost and easy to prepare, and can be applied to biosensing detection of hydrogen peroxide and environmental remediation degradation of organic pollutants such as phenols and dyes.

This invention discloses a laccase mutant, Lcc5-I479T, its expression strain, and its applications. Using laccase from *Coprinopsis cinerea* as the starting enzyme, this invention obtained the mutant gene through molecular docking and positional analysis. After induction expression in engineered bacteria containing the mutant gene, the stable laccase mutant enzyme Lcc5-I479T was obtained. Using ABTS as a substrate, the mutant enzyme Lcc5-I479T showed a stable increase in activity to 8-20 times that of the starting enzyme at 35-50℃. This mutant enzyme has potential application value in the oxidative transformation of aflatoxin.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

The invention relates to a bacillus velezensis strain resistant to high temperature and alkali, and belongs to the technical field of microbial detoxification and feed safety. The bacillus velezensis B.26 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34475 is provided aiming at the problems that in the prior art, the thermal stability of zearalenone (ZEN) degrading enzyme is insufficient, the efficiency is low in an alkaline environment, and toxic metabolites are easily generated by traditional strains. CotA laccase and peroxide reductase Prx secreted by the strain can efficiently degrade ZEN and derivatives alpha-zearalenol and beta-zearalenol, the products are low-toxicity C17H24O4 and C12H16O4, and harmful by-products such as alpha-ZEL / beta-ZEL and the like do not exist. The strain and recombinase thereof are suitable for a high-temperature alkaline environment for feed processing, and can be applied to biological detoxification of ZEN pollution in food and feed on a large scale through an immobilized carrier.

The invention belongs to the technical field of agricultural environment protection, discloses an agricultural plant pesticide residue degradation preparation based on laccase, lipase and catalase and a preparation method thereof, and belongs to the technical field of agricultural environment protection. The degradation preparation comprises the following components in percentage by mass: 0.5-3% of laccase, 0.3-2.5% of lipase, 0.2-2% of catalase, 1-5% of a stabilizer, 5-15% of a buffer agent and the balance of water, the stabilizer is one or a combination of more of sodium alginate, chitosan and glycerol, and the buffer agent is one of three buffer solutions of a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution. The preparation method comprises the following steps: weighing raw materials, preparing a buffer solution, dissolving a stabilizer, adding enzyme, stirring and mixing, carrying out temperature-controlled reaction, filtering and sterilizing and the like. According to the preparation, through the synergistic effect of the three enzymes, various pesticide residues such as organic phosphorus, organic chlorine and pyrethroids can be efficiently degraded, the stabilizer protects the enzyme activity, the buffering agent maintains the proper pH value, the preparation method is simple and environmentally friendly, and the quality safety of agricultural products can be effectively guaranteed.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to hovenia dulcis thunb powder with a hangover alleviating effect and a preparation method thereof. Comprising the following steps: (1) mixing hovenia dulcis thunb powder and water to obtain a mixed solution; (2) adding a compound enzyme into the mixed solution for enzymolysis to obtain an enzymatic hydrolysate A; (3) adding laccase into the enzymatic hydrolysate A for enzymolysis to obtain enzymatic hydrolysate B; (4) adding tannase into the enzymatic hydrolysate B for enzymolysis to obtain enzymatic hydrolysate C; (5) adding an alcohol solvent into the enzymatic hydrolysate C for extraction, carrying out solid-liquid separation, and concentrating and drying supernate to obtain hovenia dulcis thunb powder; wherein the compound enzyme comprises cellulase, pectinase and hemicellulose. According to the method, the contents of quercetin, myricetin, dihydromyricetin and spinosin in the hovenia dulcis thunb powder can be remarkably increased.

The application discloses a laccase-producing polyporus brumalis and a method for preparing laccase. The application provides polyporus brumalis CCTCC NO: M 2020809 for fermenting and preparing laccase, and the amino acid sequence of the laccase is shown as SEQ ID NO: 2. The fermentation level of the laccase is high, the fermentation level in a flask reaches 980 U / mL, and the fermentation level in a 70L fermenter reaches 880 U / mL. In order to ensure the food safety of the laccase, the strain and the preferred medium components of the laccase are edible. In addition, the laccase obtained by the application can catalyze the polymerization of film-forming monomers to generate a copolymer film, and is a method for synthesizing a copolymer film by using a biological enzyme catalyst.

The invention discloses a biological agent for efficiently degrading mycotoxin, a method and application. Belongs to the technical field of enzyme biological preparations. The novel laccase is obtained from a Ganoderma lucidum culture solution and is named as GL-Lac, the amino acid sequence of the novel laccase is shown as SEQ ID NO.1, the novel laccase can effectively degrade AFB1, ZEN, DON, T-2 and other mycotoxins, non-phenolic toxin-AFB1 can be efficiently oxidized without additional mediators, in addition, by means of a composite induction strategy, the degradation rates of AFB1, ZEN, DON and T-2 toxins are remarkably increased and reach 80% or above, 90% or above, 78% or above and 75% or above respectively, and the novel laccase is suitable for industrial production. And a technical support is provided for degradation of mycotoxin.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

The invention discloses a protein-based multifunctional food preservative film as well as a preparation method and application thereof, and belongs to the technical field of functional modification of natural polymer materials. The preparation method of the protein-based multifunctional food preservative film comprises the following steps: firstly, carrying out hydrolysis pretreatment on zein by utilizing the hydrolysis effect of protease, then grafting catechol into a protein structure by utilizing an acyl transfer reaction of TGase, and finally, carrying out functional grafting modification on the zein by utilizing chitosan by utilizing an oxidation reaction of laccase, so as to obtain the protein-based multifunctional food preservative film. The physical and mechanical properties, the stability and the antibacterial activity of the zein film are further improved, and the protein film is endowed with antioxidant activity, so that the protein-based multifunctional food preservative film material suitable for food preservation is prepared. When the protein-based multifunctional food fresh-keeping film is used for fruit fresh-keeping treatment, the shelf life is remarkably prolonged, and the protein-based multifunctional food fresh-keeping film has a good fresh-keeping effect.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention provides a liquid laccase protective agent and application thereof, and belongs to the technical field of bioengineering. According to the liquid laccase protective agent, a NaOH buffer solution with the pH value of 10.0 is used as a solvent, and solutes comprise trehalose, bovine serum albumin, glycine, Tween 80, sodium alginate, glycerin and copper chloride; the liquid laccase protective agent can effectively reduce the enzyme activity loss of laccase in the storage and transportation processes, prolong the storage life of the laccase and improve the application effect of the laccase, so that the application cost of the laccase is reduced, the application of the liquid laccase in the fields of food processing, papermaking industry, textile industry, industrial wastewater treatment and the like is facilitated, and the liquid laccase protective agent has good practicability.

Provided is a laccase derived from a strain of Versicolor versicolor. The present invention relates to a polypeptide comprising the mature polypeptide of the amino acid sequence of SEQ ID NO: 2. Compared to existing laccases, the polypeptide of the present invention exhibits a higher benzo[a]pyrene removal effect.

The invention discloses iron-based nano laccase and a preparation method and decoloration application thereof, and relates to the technical field of iron-based nano laccase preparation, and the preparation method comprises the following steps: S1, iron-based nano carrier pretreatment; s2, synergistically modifying the iron-based nano-carrier; s3, preparing a laccase buffer solution; s4, carrying out ultrasonic and microwave synergistic in-situ loading; s5, step-by-step curing is carried out; s6, gradient purification; and S7, vacuum drying. According to the method, the binding effect of the laccase and the carrier is greatly improved, the problems of low laccase loading rate and large enzyme activity loss in the prior art are effectively improved, the product has excellent catalytic activity and stability, meanwhile, the binding firmness of the laccase and the carrier is further enhanced through the step-by-step curing process, laccase falling in the using process is reduced, and the service life of the product is prolonged. And in combination with excellent magnetic responsiveness of the iron-based nano-carrier, rapid separation, recovery and reutilization of the product are realized, and the practical application cost is greatly reduced.

This invention discloses a laccase gene PmLAC31 derived from *Pinus massoniana* and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the *PmLAC31* laccase gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. This invention also provides biomaterials containing the gene, and the application of this gene or biomaterials in improving plant drought resistance and / or lignin content. Experiments show that heterologous overexpression of the PmLAC31 gene in poplar significantly promotes lignin deposition and enhances the plant's drought tolerance. This invention provides important gene resources and application pathways for molecular breeding of forest trees for stress resistance.

The invention belongs to the field of polishing solutions, and particularly relates to a green polishing solution based on an enzymatic Fenton system. The 4H-SiC substrate polishing solution comprises a component A and a component B, the effective component of the component A is an enzyme-catalyst co-immobilized abrasive material, and the enzyme-catalyst co-immobilized abrasive material comprises an abrasive material matrix, laccase and an iron-based catalyst, and the laccase and the iron-based catalyst are loaded on the abrasive material matrix; the active ingredient of the component A is a phenol substrate, the active ingredient of the component B is a phenol substrate, after the component A and the component B are mixed, laccase catalyzes oxidation of the phenol substrate to generate H2O2 in situ, the H2O2 and an iron-based catalyst are subjected to a Fenton reaction, and hydroxyl free radicals are generated at a polishing interface in situ. According to the invention, continuous, efficient and stable supply of hydroxyl radicals is ensured through enzymatic endogenous hydrogen peroxide circulation and catalyst regeneration circulation, so that the polishing solution can realize long-time stable polishing under a nearly neutral condition, and has the advantages of environmental protection, high polishing efficiency, good surface quality and the like.

The application relates to the technical field of sponge production, and discloses a production process of high-density elastic environment-friendly sponge. The application realizes gradient foaming under laminar flow control by designing pH-sensitive three-layer core-shell laccase microcapsules with a particle size of 3-4 microns and an enzyme activity of 180-220 U / g, combining a bio-based polyester matrix with a water and liquid CO2 composite foaming system; the process core comprises the following steps: enzyme triggering solidification, microcapsule rupture triggered by pressure relief at 55-65 DEG C, crosslinking completed by three-stage gradient heating at 75-85 DEG C; a double-gradient structure with a surface layer bubble hole density of 150-190 cells / cm3 and a core layer bubble hole density of 75-95 cells / cm3 is formed; finally, the density is 90-110 kg / m3, the compression permanent deformation is less than or equal to 5%, VOCs is less than or equal to 0.02 mu g / m3, and the biodegradation rate is greater than 90%; the process solves the problems of poor durability and insufficient environmental protection of traditional sponges.

The present application belongs to the technical field of biological remediation and solid organic waste treatment and resource, and particularly relates to application of phenolic compounds directly degradable by laccase in compost and composting method for promoting humification. The present application uses phenolic compounds directly degradable by laccase in compost, and the composting process incubates a large number of microbial communities. When nutrients are sufficient, most of the microorganisms will show competitive response; and under stress environment, the microorganisms tend to be synergistic. In the early stage of the composting reaction, the humification process in the compost is often slow, and the microorganisms will make full use of the humic acid precursor, slowing down the composting. The toxicity of the phenolic compounds directly degradable by laccase can promote the synergistic function of the microorganisms in the compost, and because it can enhance the expression of laccase, it can promote the co-metabolic effect of laccase on pollutants and lignin, degrade the pollutants while promoting the humification of the compost, and protect the environment.

The invention provides a biomimetic enzyme functional composite membrane constructed by microfluidics and application of the biomimetic enzyme functional composite membrane in removal of water pollutants. According to the composite membrane, lipidosome vesicles embedded with laccase molecules are prepared through a microfluidic method, a functional coating is formed by sodium alginate, and the functional coating and a porous PVDF supporting membrane are compounded to form a multi-layer membrane structure. The prepared biomimetic membrane simulates the gradient structure of a biological water channel, achieves the synergistic effect of enzyme catalytic degradation and physical filtration, has high selectivity and high permeation flux, and solves the technical problem that'flux-selectivity 'of a traditional membrane is difficult to consider. The membrane material is derived from natural renewable resources, the preparation process is green and environment-friendly, and the membrane shows efficient purification capacity in the aspect of removing heavy metal ions and organic dye pollutants and has excellent anti-pollution and self-cleaning characteristics.

The application belongs to the technical field of bioengineering, and particularly relates to a high-temperature-resistant laccase mutant obtained by site-directed mutation through an overlapping PCR technique, and preparation and application thereof. The laccase mutant is obtained on the basis of a wild-type laccase shown in SEQ ID NO. 1, and at least one of D221Y, E231D, Y441H or Y441F mutations occurs, and the mutant enzyme activity and the thermal stability at 80 DEG C are both improved to different degrees relative to the wild type, which is beneficial to expand the application in the industrial fields of degrading lignocellulose, paper pulp bleaching and the like.