369 results about "Laccase" patented technology

The invention relates to the technical field of carbon black, in particular to a preparation process of high-adsorbability carbon black, which comprises the following steps: performing pore-forming treatment on carbon black to obtain a porous carbon black material, then treating the porous carbon black material with a first mixed amino acid solution to obtain a carbon black material loaded with a first mixed amino acid adsorption layer, and drying the carbon black material to obtain the high-adsorbability carbon black. And treating the carbon black material with a second mixed amino acid solution to obtain a carbon black material loaded with a second mixed amino acid adsorption layer, and treating the carbon black material loaded with mixed amino acids with laccase and tyrosinase. The carbon black product obtained by the preparation process has high adsorbability, and is used for treating metal ions in wastewater, so that the removal rate of the metal ions is greatly improved.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

The invention discloses a laccase mutant and application thereof in desizing wastewater treatment. Wild laccase is used as a female parent, and a high-enzyme-activity mutant is obtained through multiple groups of mutations. The temperature of the desizing wastewater subjected to centralized treatment is 15-50 DEG C, the pH value is 4-7, and the application condition is wide. Through verification, the enzyme activity of the laccase mutant disclosed by the invention is more than 10U / mg under the environment that the temperature is 25 DEG C and the pH value is 6.0 (common conditions for centralized treatment of desizing wastewater). Even under the extreme conditions that the pH is 4.0 or 7.0 and the reaction temperature is 15 DEG C or 50 DEG C, the laccase variant can still keep the enzyme activity of 5.5 U / mg or above, and therefore the laccase variant can be used for treating desizing wastewater with the temperature being 15-50 DEG C and the pH being 4-7 in a large scale. Compared with wild type laccase with lower enzyme activity, the laccase mutant obtained by the invention has the advantages that the enzyme activity is obviously improved and can be improved by 10 times at most, and the wastewater treatment cost is obviously reduced.

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention belongs to the technical field of gene engineering of enzymes, and particularly relates to high-temperature-resistant laccase as well as preparation and application thereof. According to the present invention, the laccase gene derived from Priesta aryabhattai is subjected to molecular modification through the rational design so as to obtain the S281E mutant and the S281E / N387D mutant; the enzyme activities of the mutants S281E and S281E / N387D are respectively improved by 72.7% and 40.4% compared with the enzyme activities of the wild type mutants S281E and S281E / N387D; under the conditions that the temperature is 70 DEG C and the temperature is kept for 60 minutes, the residual enzyme activity of WT is 25.2%, the residual enzyme activity of S281E is 45.1%, and the residual enzyme activity of S281E / N387D is 80.2%.

The invention discloses a supported ozone catalyst for printing and dyeing wastewater treatment and a treatment process, and relates to the technical field of wastewater treatment, the supported ozone catalyst comprises an environmental response type porous medium, an active center and a biological enzyme modification layer; the environment-responsive porous medium is based on poly (N-isopropylacrylamide) grafted and modified mesoporous alumina, the initial aperture of the mesoporous alumina is 10-15nm, the grafting rate of poly (N-isopropylacrylamide) is 12-20%, and an active center is composed of a transition metal active center and quantum dots modified on the transition metal active center. The biological enzyme modified layer is obtained by carrying out deep biological modification on the surface of the catalyst by using laccase with oxidation-reduction activity. According to the invention, an environment-responsive porous medium is constructed by grafting mesoporous alumina with poly (N-isopropylacrylamide), so that the problems that a traditional fixed-aperture catalyst is low in mass transfer efficiency and poor in adaptability to working conditions, and the pollutant release efficiency is difficult to effectively improve under different temperature and pH conditions are solved.

The invention belongs to the technical field of gene engineering, and relates to high-temperature-resistant laccase, a gene and application of the high-temperature-resistant laccase in mycotoxin degradation. The amino acid sequence of the laccase TtLac-4 is as shown in SEQ ID NO: 1. On the basis of wild laccase, site-directed mutagenesis modification is carried out through artificial rational design, and an amino acid sequence of the modified high-temperature-resistant laccase and a nucleotide sequence of the artificially optimized high-temperature-resistant laccase gene are obtained. The high-temperature-resistant laccase obtained through gene coding of the high-temperature-resistant laccase has the remarkably-improved high-temperature-resistant characteristic, and the optimum temperature reaches 90 DEG C. The degradation rate of the laccase TtLac-4 obtained by the invention on mycotoxin aflatoxin B1 and zearalenone reaches 100%. The invention lays a foundation for a green degradation process and application of mycotoxin degradation.

The invention belongs to the technical field of nano-enzymes, and particularly relates to a CuPH (at) KMO composite nano-enzyme material as well as a preparation method and application thereof. Aiming at the problems of poor catalytic activity, complex steps, high raw material cost and low biocompatibility of nano-enzyme prepared by the existing method, the preparation method comprises the following steps: by taking terephthalic acid and histidine as dual-coordination copper-based nano-enzyme as a precursor, introducing manganese oxide in situ under a hydrothermal condition; and the flower-like nano mimic enzyme which is uniform in size and has high laccase-like catalytic characteristics at the same time is synthesized. The composite nano-enzyme provided by the invention can catalytically oxidize phenol amine organic compounds under the condition that the pH value is 3-9, can realize the detection of the content of ergothioneine in cosmetics and nutritional supplement capsules, and can be applied to the field of cosmetic and food safety monitoring.

The invention relates to a method for determining potassium by using a nano-enzyme-based colorimetric probe, and the probe and application thereof. The invention relates to a method for determining potassium by using a nano-enzyme-based colorimetric probe, which comprises the following steps of: synthesizing copper and cerium-containing phosphotungstic acid nano-enzyme by using phosphotungstic acid, cerous nitrate and copper chloride as precursors through a hydrothermal method, detecting the absorbance of K < + > standard solutions with different concentrations by using the copper and cerium-containing phosphotungstic acid nano-enzyme as a probe, establishing a quantitative relation between the absorbance and the K < + > concentration, drawing a standard curve, obtaining a regression equation, and determining the potassium content by using the regression equation. And detecting the absorbance of the sample determination standard solutions with different concentrations, and obtaining the K < + > content of the sample through the regression equation. According to the method, the nano-enzyme (Cu, Ce-PWs) is prepared by using tungsten-molybdenum heteropoly acid with high negative charges, unique chelating properties and various structures and Ce < 4 + > and Cu < 2 + > as raw materials, strong mimic enzyme laccase activity is generated through the synergistic effect of Ce atoms and Cu atoms, and the activity of the mimic enzyme laccase is further improved in the presence of H2O2.

The invention relates to the technical field of food processing, in particular to a chicken premade dish and a preparation method thereof. By innovatively integrating a laccase-chequer-shaped indocalamus leaf cross-linking system and a double-frequency ultrasonic pretreatment process, the water retention, bacteriostasis and flavor regulation performance of the chicken premade dish can be synchronously improved, and the effect cannot be replaced by other enzymes such as papain; besides, double-frequency ultrasonic pretreatment is adopted, dynamic balance of deep penetration-surface tenderization is achieved through frequency alternation, and compared with single-frequency or traditional soaking, the method can better cooperate with laccase catalysis to directionally cross-link the preservative, and the effects of water retention, bacteriostasis and flavor regulation and control are achieved.

The present invention provides a azelastine hydrochloride nasal spray and a preparation method thereof, belonging to the technical field of nasal sprays. The azelastine hydrochloride nasal spray is composed of the following components by mass percentage: 0.1% of azelastine hydrochloride, 0.3 - 0.4% of a composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower, 0.005 - 0.01% of a stabilizer, 0.002 - 0.01% of a preservative, 0.95 - 1.05% of a thickening agent, 0.7 - 0.8% of a pH regulator, 0.45 - 0.48% of sodium chloride, and the balance of purified water; the composite water extraction fermentation product of chamomile, houttuynia cordata, and peony flower is prepared by first subjecting a mixture of chamomile, houttuynia cordata, and peony flower to water extraction to obtain a water extract and then subjecting it to composite enzyme fermentation. The composite enzyme includes cellulase, pectate lyase, and laccase. The present invention can effectively reduce the irritation to the eyes during use and ensure safer and more reassuring use.

The application discloses a kind of fungal laccase mutant Lcc5-G and its expression strain and application.The application is based on the fungal laccase from Coprinopsis cinerea heterologous expression since, by site-directed mutagenesis, obtain mutant gene.After fungal induction expression containing mutant plasmid, obtain laccase mutant enzyme Lcc5-G with improved specific activity, stability and AFB1 removal efficiency.When ABTS is used as substrate, the specific activity of the mutant is improved by 3 times, and the stability of the mutant enzyme is improved to 5 times of the original enzyme at 45 DEG C and pH 7.The mutant has potential application value in oxidizing aflatoxin.

The invention relates to the technical field of sewage treatment, and particularly discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The invention discloses a high-suspended-matter sewage treatment agent for an oil extraction plant. The high-suspended-matter sewage treatment agent comprises 40-60 parts of modified bentonite; 5-15 parts of ferrous sulfate; 5-15 parts of aluminum ferric sulfate; 1 to 5 parts of ethylenediamine tetraacetic acid; 10 to 20 parts of an active biochar material; 3 to 8 parts of chitosan; 5 to 10 parts of a flocculating agent; the modified bentonite is obtained by modifying bentonite through laccase and nano zero-valent iron; the active biochar material is obtained by taking waste biomass as a raw material and carrying out semi-carbonization and activation treatment. The sewage treatment agent disclosed by the invention can be used for purifying high-suspended-matter sewage generated by oil extraction plants and oil wells, has high removal efficiency on suspended matters, and can effectively remove organic humus, so that the content of the suspended matters in the treated sewage reaches the standard.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The invention discloses a microbial decomposing inoculant for straws and a preparation method thereof, and belongs to the technical field of microorganisms. The microbial decomposing inoculant for straw is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of a nano-porous diatomite carrier, 5-7 parts of a humectant, 2-3 parts of humic acid chelated trace elements and 2-4 parts of an inducer. The preparation method comprises the following steps: screening talaromyces thermophilus, Guangxi thermoactinomycetes and arthrobacter habitualis to form a functional microbial liquid, adding a laccase-protease compounding system to cooperate with the physical adsorption effect of diatomite, and adding humic acid chelated trace elements to form an ion exchange network with polyglutamic acid; straw components are efficiently degraded through a triple mechanism of'enzymatic degradation-element supplementation-microenvironment regulation and control ', the decomposition process is accelerated, and the straw compost quality is improved.

The present disclosure provides a porous microcarrier loaded with laccase, a preparation method and an application. The porous microcarrier loaded with laccase includes: a hydrogel porous microcarrier; laccase, which is loaded on the hydrogel porous microcarrier by covalent bonding; wherein the hydrogel porous microcarrier is a methacrylated hydrogel microcarrier filled with chitosan, providing a loading site for laccase.

The invention belongs to the technical field of biology, and particularly discloses application of laccase AmLac in degradation of biogenic amines. The amino acid sequence of the laccase is shown as SEQ ID NO.1. The laccase has different degrees of degradation effects on the biogenic amines such as histamine, spermidine, tyramine, phenylethylamine and putrescine, and the degradation rates of the histamine and the putrescine both reach 100%. The enzyme is stable in enzymatic property, has alcohol tolerance and acid tolerance, has certain adaptive capacity to food matrixes, and can effectively degrade biogenic amines in wine and reduce potential hazards of the biogenic amines to human bodies, so that the safety and stability of products are improved. Therefore, the AmLac has a wide application prospect in the food industry, and especially when the AmLac is used for treating food containing high biogenic amine, the safety quality of the food can be remarkably improved.

The embodiment of the invention provides a fluorescent probe, an application method thereof and a fluorescent probe kit. According to the fluorescent probe provided by the embodiment of the invention, the laccase has relatively high sensitivity and specificity, and the laccase can be accurately detected under the condition that the laccase concentration is relatively low, so that the laccase detection accuracy is effectively improved. When the fluorescent probe provided by the embodiment of the invention is used for laccase detection, the detection cost can be reduced to 1 yuan per time, and compared with the existing ABTS colorimetric method, the cost advantage is better.

The invention relates to high-strength degradable self-healing hydrogel and a preparation method thereof, and belongs to the technical field of high polymer materials. The hydrogel is prepared from the following components in percentage by mass: 15 to 20 percent of carboxymethyl cellulose, 10 to 14 percent of acrylic acid, 15 to 20 percent of N-isopropylacrylamide, 4 to 8 percent of tannic acid, 2 to 4 percent of borax, 0.06 to 0.1 percent of laccase, 0.5 to 1 percent of N, N-methylene bisacrylamide, 0.4 to 0.6 percent of potassium persulfate and the balance of phosphate buffer solution. According to the hydrogel disclosed by the invention, through dual-network structure design, dynamic covalent bond crosslinking and enzyme catalysis green synthesis, collaborative optimization of high strength, high self-healing property, degradability and multi-responsiveness is realized, and the problems of poor mechanical property, low repairing efficiency, environmental unfriendliness and the like of traditional hydrogel are solved; the method has wide application potential in the fields of biomedicine, flexible electronics, intelligent packaging and the like.

A fuel pellet is disclosed. The fuel pellet comprises a plant fiber and a lignin modifying enzyme exogenous to the plant fiber. In some embodiments, the plant fiber is a Tracheophytes plant fiber. In some embodiments, the plant fiber is a monocot plant fiber. In some embodiments, the plant fiber is a Poaceae plant fiber. In some embodiments, the plant fiber is a Saccharum plant fiber. In some embodiments, the plant fiber is sugarcane bagasse plant fiber. In some embodiments, the pellet does not contain a binding agent. In embodiments without a binding agent, the fuel pellet is held together by lignin, which is present in the plant fiber and modified to hold the plant fibers together. In some embodiments, the lignin modifying enzyme is a lignase. In some other embodiments the lignin modifying enzyme is a laccase. The disclosure further provides a method of making a fuel pellet.

The present invention relates to a mycotoxin degrading agent and its application, belonging to the field of agricultural biology. The present invention provides a highly efficient mycotoxin degrading agent that can be used for mycotoxin degradation. The mycotoxin degrading agent of the present invention comprises bacterial laccase Lac_P.E. and an artificial mediator, and can efficiently degrade mycotoxins, including aflatoxin B1 and zearalenone, and is widely used in the field of mycotoxin detoxification.

The invention relates to a method for efficiently degrading phenolic acid autotoxic substances in continuous cropping of crops by using biochar immobilized laccase. Pyrolyzing the tobacco stem and straw powder in a muffle furnace to obtain tobacco stem and straw biochar BC; the preparation method comprises the following steps: soaking BC and potassium carbonate in a mass ratio of 1: 4 for 2-4 hours, drying, pyrolyzing in a muffle furnace after drying, air-cooling to room temperature, washing with deionized water, drying and sieving to obtain potassium carbonate modified tobacco stem and straw biochar CBC; dispersing CBC in an HNO3 solution, mixing for 3-5 hours at the speed of 300rpm and the temperature of 30 DEG C, washing with deionized water until the pH value is 7, drying overnight, and storing at room temperature to obtain functionalized CBC; placing the functionalized CBC in a 50mM phosphate buffer solution for 20-30 hours, adding 1.0% w / v glutaraldehyde with the same volume as the functionalized CBC, oscillating at room temperature, washing with redundant glutaraldehyde deionized water, and drying overnight to obtain glutaraldehyde-containing CBC; and carrying out enzyme immobilization on the glutaraldehyde-containing CBC and a 5-10U / mL laccase solution according to a solid-to-liquid ratio of 1g: 10mL at 25-30 DEG C to obtain the immobilized laccase. Autotoxic substances are effectively degraded, the yield and quality of peppers are improved, and ecological restoration of soil is promoted.

The present invention belongs to the field of biodegradation of mycotoxins, and specifically relates to a complex enzyme preparation for degrading aflatoxins, a complex enzyme preparation solution, and its preparation method and application. The complex enzyme preparation comprises: 35-45 wt% tyrosinase, 20-30 wt% cellulase, 8-12 wt% pectinase, 8-12 wt% xylanase, and 10-20 wt% mushroom laccase. Compared with existing chemical and biological detoxification technologies, the complex enzyme preparation of the present invention contains safe and non-toxic components, is simple to use, and has low economic costs. After treatment with the enzyme preparation solution, the cellulose and hemicellulose in the peanut meal are initially decomposed, making it easier for animals to digest and absorb, and having higher nutritional value and better economic benefits.

The invention relates to the technical field of nano-enzymes, and discloses a copper-based nano-enzyme with laccase and peroxidase double-enzyme activity and preparation and application thereof, and the copper-based nano-enzyme is a nano-material prepared by forming MOF through solvothermal reaction of copper ions and diamino terephthalic acid and then performing electrostatic adsorption on the MOF and polyoxometallate. The copper-based nano enzyme has high-efficiency laccase-like activity and peroxidase-like activity at the same time through the combination and synthesis process of the copper ion cluster and the organic ligand. The invention further discloses a preparation method of the copper-based nano-enzyme, peroxidase activity detection and application of the copper-based nano-enzyme to degradation and determination of dichlorophenol. The copper-based nano-enzyme provided by the invention has high catalytic activity and stability, is low in cost and easy to prepare, and can be applied to biosensing detection of hydrogen peroxide and environmental remediation degradation of organic pollutants such as phenols and dyes.

The invention discloses an energy-saving pulping method based on pretreatment strengthening and segmented enzyme activation, and belongs to the technical field of pulping and papermaking. According to the pulping method provided by the invention, p-toluenesulfonic acid is added in pretreatment to adjust pH and strengthen enzyme activity, and enzyme is wrapped by using a temperature or pH response type carrier, so that pectinase, cellulase and laccase can be controlled to be released and activated in a proper treatment stage. By accurately controlling the enzyme action sequence, substrate competition is avoided, enzyme inactivation is reduced, the enzymolysis efficiency and the utilization rate are effectively improved, and the COD content of wastewater is reduced; and two-stage pulping and p-toluenesulfonic acid are combined to soften fibers synergistically, so that the power consumption per ton of pulp is reduced. According to the invention, fiber fibrillation is promoted through enzyme treatment, and the tensile strength and the water retention value are effectively improved; the laccase catalyzes crosslinking of lignin, so that the wet strength is remarkably improved. The paper pulp provided by the invention gives consideration to physical properties and environmental protection requirements of high-strength packaging paper manufacturing, and has a wide application prospect.

This invention discloses a laccase mutant, Lcc5-I479T, its expression strain, and its applications. Using laccase from *Coprinopsis cinerea* as the starting enzyme, this invention obtained the mutant gene through molecular docking and positional analysis. After induction expression in engineered bacteria containing the mutant gene, the stable laccase mutant enzyme Lcc5-I479T was obtained. Using ABTS as a substrate, the mutant enzyme Lcc5-I479T showed a stable increase in activity to 8-20 times that of the starting enzyme at 35-50℃. This mutant enzyme has potential application value in the oxidative transformation of aflatoxin.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.