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Magnetic nanoparticle-based immobilized laccase and ionic liquid composite particle and application thereof

A technology for immobilizing laccase and magnetic nanometers, which is applied in the directions of immobilization on or in inorganic carriers, chemical instruments and methods, microsphere preparation, etc. problem, to achieve the effect of improving stability, low cost and high removal efficiency

Inactive Publication Date: 2013-04-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ionic liquids have high viscosity. When extracting organic pollutants in water, the two-phase interface is small and recovery is difficult, which limits the effective use of ionic liquids.

Method used

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  • Magnetic nanoparticle-based immobilized laccase and ionic liquid composite particle and application thereof
  • Magnetic nanoparticle-based immobilized laccase and ionic liquid composite particle and application thereof
  • Magnetic nanoparticle-based immobilized laccase and ionic liquid composite particle and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Mix 1.0mL carbonyl iron, 20mL octyl ether and 12.9g oleic acid, heat to 100°C and react for 3 hours, then cool to room temperature, add 1.7g anhydrous trimethylamine oxide, under nitrogen protection, heat to 130°C, react 3 Hours later, cool to room temperature, add 100mL ethanol, centrifuge, and precipitate as magnetic γ-Fe 2 o 3 Nanoparticles, which are dispersed in n-hexane to obtain magnetic nano-γ-Fe with a concentration of 0.5g / mL 2 o 3 solution; 1.0mL magnetic nano-γ-Fe 2 o 3 Solution, 2.0mL polyoxyethylene nonylphenyl ether (polymerization degree 520) and 20mL n-hexane ultrasonic dispersion for 10 minutes, 0.1mL ammonia water, 0.4mL tetraethyl orthosilicate, stirred at room temperature for 36 hours, added 0.5mL N-β-(aminoethyl)-γaminopropyltrimethoxysilane, at room temperature, stirring reaction for 24 hours, centrifuged, the solid phase is the surface amino functionalized nano-magnetic silica particles; 1g surface amino Functionalized nano-magnetic silica p...

Embodiment 2

[0032] Mix 1.0mL carbonyl iron, 50mL octyl ether and 6.45g oleic acid, heat to 90°C for 4 hours, cool to room temperature, add 3.4g anhydrous trimethylamine oxide, under nitrogen protection, heat to 150°C, reaction 1 Hours later, cool to room temperature, add 300mL ethanol, centrifuge, and precipitate as magnetic γ-Fe 2 o 3 Nanoparticles, which are dispersed in n-hexane to obtain magnetic nano-γ-Fe with a concentration of 0.5g / mL 2 o 3 solution; 1.0mL magnetic nano-γ-Fe 2 o 3 Solution, 3mL polyoxyethylene nonylphenyl ether (polymerization degree 520) mixed with 30mL n-hexane and ultrasonically dispersed for 10 minutes, added 0.1mL ammonia water and 0.3mL tetraethyl orthosilicate, stirred and reacted at room temperature for 48 hours, then added 0.3mL of 3-aminopropyltriethoxysilane, stirred and reacted for 48 hours at room temperature, centrifuged, the solid phase was nano-magnetic silica particles with surface amino-functionalized; 3g of nano-magnetic silica particles with...

Embodiment 3

[0036] Mix 1.0mL carbonyl iron, 30mL octyl ether and 10.75g oleic acid, heat to 140°C and react for 1 hour, then cool to room temperature, add 2.3g anhydrous trimethylamine oxide, under nitrogen protection, heat to 120°C, reaction 3 Hours later, cool to room temperature, add 160mL ethanol, centrifuge, and precipitate as magnetic γ-Fe 2 o 3 Nanoparticles, which are dispersed in n-hexane to obtain magnetic nano-γ-Fe with a concentration of 0.5g / mL 2 o 3 solution; 1.0mL magnetic nano-γ-Fe 2 o 3 Solution, 3.0mL polyoxyethylene nonylphenyl ether (polymerization degree 520) mixed with 30mL n-hexane and ultrasonically dispersed for 10 minutes, added 0.1mL ammonia water and 0.6mL tetraethyl orthosilicate, stirred at room temperature for 24 hours, Then add 0.9mL N-β-(aminoethyl)-γaminopropyltrimethoxysilane, stir and react for 12 hours at room temperature, and centrifuge to obtain nano-magnetic silica particles whose solid phase is surface amino-functionalized; Mix 1 g of surface ...

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Abstract

The invention discloses a magnetic nanoparticle-based immobilized laccase and an ionic liquid composite particle, a preparation method thereof and application in removal of contaminants in water. The preparation method comprises the steps that carbonyl iron is taken as an iron source and is synthesized to be gamma-Fe2O3 nano-particles through a chemical precipitation method; the magnetic nanoparticles are taken as cores, tetraethoxysilane and a silane coupling agent are taken as a silicon source, and by means of a sol-gel method, surface amino-functionalized nano core-shell type magnetic silicon dioxide is prepared; 1-ethyl-(3-dimethyllaminopropyl) carbodiie hydrochlide is taken as a coupling agent, and laccase is bonded on the surface of the nano core-shell type magnetic silicon dioxide in a covalent bond manner to obtain the immobilized laccase of the magnetic nanoparticle silicon dioxide particles; and then the immobilized laccase is reacted with a functionalized ionic liquid which is synthesized by reacting N,N-carbonyldiimidazole and an ionic carboxy contained liquid to prepare the magnetic nanoparticle immobilized laccase and ionic liquid composite particle, and the composite particle is applied to the removal of the contaminants in water.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a composite microparticle based on magnetic nano-immobilization laccase and ionic liquid, its preparation method and its application in removing pollutants in water bodies. Background technique [0002] With the rapid development of the economy, the pollution of the water environment is becoming more and more serious, and organic pollutants accumulate and persist day by day, which has seriously endangered the health of the ecosystem and the sustainable development of human society. In addition to being difficult to degrade, they also have high biological toxicity and carcinogenic, teratogenic, and mutagenic properties. Therefore, the research and development of efficient and economical new technologies for wastewater treatment has become a focus of concern for environmental protection workers all over the world. [0003] Laccase is a copper-containin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C02F3/00C12N11/14
Inventor 安小宁
Owner SOUTH CHINA UNIV OF TECH
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