11 results about "Ascorbate Oxidase" patented technology

The application relates to a buffer reagent, a kit, a branched-chain alpha-keto acid or a branched-chain amino acid detection method in the technical field of biological detection. The buffer reagent comprises 15-25 mM, pH 8.5-9.5 Tris-HCl buffer solution, 10-50 g / L trehalose, 0.1-1.5 g / L Proclin 300, 0.1-0.5 g / L Triton X-100, 0.1-1.5 g / L sodium salt of EDTA, 1-10 kU / L ascorbic acid oxidase and 0.5-3 g / L bovine serum albumin. The buffer reagent is suitable for preparation of a reagent containing leucine dehydrogenase, the reagent containing the buffer reagent and the leucine dehydrogenase and the kit prepared by using the reagent can effectively eliminate interference problems in the process of being used for branched-chain alpha-keto acid, branched-chain amino acid detection, and has good correlation with mass spectrometry.

The invention provides a lipoprotein sub-component phospholipid detection kit, and belongs to the field of in-vitro diagnostic reagents, and in particular, the lipoprotein sub-component phospholipid detection kit provided by the invention comprises a reaction reagent, the reaction reagent comprises a buffer solution, a surfactant, magnesium chloride, 4-aminoantipyrine, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3, 3, 4-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5- The preservative is prepared from 2, 5-dimethoxyaniline sodium salt, phospholipase D, choline oxidase, peroxidase, ascorbic acid oxidase, a protective agent and a third preservative. The lipoprotein subfraction phospholipid detection kit provided by the invention can be used for determining the content of lipoprotein subfraction phospholipid by using a VAP system, simultaneously realizes simultaneous detection of a plurality of lipoprotein subfraction phospholipid items for the first time, and has the advantages of high precision and high accuracy.

The application belongs to the technical field of nanomaterials, and provides ascorbic acid oxidation mimetic enzyme and preparation thereof. The application adopts a methanol solution of zinc nitrate, copper acetylacetonate and 2-methyl imidazole to perform a hydrothermal reaction, and obtains the ascorbic acid oxidation mimetic enzyme by oxygen isolation calcination of a hydrothermal product. The copper-nitrogen-carbon nanomaterial obtained by the application has a rhombohedron structure, can specifically oxidize ascorbic acid, and can be applied to ascorbic acid content detection, oxidized ascorbic acid preparation and dehydroascorbic acid preparation as ascorbic acid oxidation enzyme mimetic enzyme. The nanomaterial can maintain ascorbic acid oxidation mimetic enzyme activity under acid, alkaline and high temperature conditions; the steady-state kinetic parameter (Michaelis constant is 0.012 mM) is better than that of natural ascorbic acid oxidation enzyme, and the ascorbic acid oxidation mimetic enzyme activity of the material has a broad application prospect in the fields of nanozyme, nanotechnology and biological analysis detection.

The application provides an electrochemical uric acid test paper and a uric acid detection method. The electrochemical uric acid test paper has simple structure, low cost, easy manufacturing process, rapid reaction, sensitive detection, wide storage temperature, wide use temperature, wide effective period, accurate result, simple operation, etc. The uric acid detection method distinguishes the test current of ascorbic acid by different time and different excitation signals, subtracts the current signal of ascorbic acid from the current signal of uric acid and ascorbic acid, eliminates the interference of ascorbic acid, is simple to operate and low in cost, does not need to add other material components, does not need the participation of uric acid oxidase and ascorbic acid oxidase, directly distinguishes the current signal by different voltages, is short in test time, reduces the complex data correction process, maintains good repeatability of data, is simple in logic operation, basically has no influence on the repeatability of test signals, and can effectively remove the interference of ascorbic acid.

The invention discloses a method for synthesizing nano-enzyme with ascorbic acid oxidase and catalase double-enzyme activity based on an iron-doped zinc-based metal organic framework material. The zinc-based metal organic framework material is a porous coordination high-molecular polymer crystal formed by coordination of zinc ions and organic ligands. The metal organic framework material is rich in active sites, large in specific surface area, adjustable in structure, large in porosity and remarkable in catalytic performance. Furthermore, the metal organic framework material is used as a precursor and is calcined and pyrolyzed in inert gas such as argon or nitrogen or air to synthesize a novel porous material, and the novel porous material has relatively large specific surface area, good thermal stability and chemical stability and unique catalytic performance. The invention discloses a method for synthesizing a unique nano-enzyme with ascorbic acid oxidase and catalase double-enzyme activity by using an iron-doped zinc-based metal organic framework material as a precursor and calcining the iron-doped zinc-based metal organic framework material in an inert gas atmosphere at a high temperature, and the method has the advantages of simplicity in operation, simple procedure and the like.

The invention provides a copper calcium phosphate nano-enzyme fibroin antibacterial dressing as well as a preparation method and application thereof, and belongs to the technical field of material preparation. CCP HNS nano-enzyme with ascorbic acid oxidase is prepared through a coprecipitation method, nano-enzyme particles with controllable morphology, stable structure and excellent catalytic performance are obtained by regulating and controlling the ratio of copper to calcium and reaction conditions, and then the nano-enzyme particles are uniformly loaded on a natural protein material silk fibroin (SF), so that the nano-enzyme is prepared. The calcium copper phosphate nano-enzyme fibroin antibacterial dressing is obtained; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has an excellent antibacterial effect, is relatively low in cytotoxicity, maintains good cell viability and shows good biocompatibility; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has obvious advantages in the aspects of infection control, inflammation relief and tissue regeneration, can greatly shorten the wound closing time, shows good healing promoting ability, and has good practicability.

The invention provides a whole blood treatment agent, a kit and application of the whole blood treatment agent in detection of calprotectin, and relates to the technical field of in-vitro detection, the whole blood treatment agent comprises 5-15 mmoL / ml of ammonium chloride or 0.01-0.05 mg / ml of ascorbic acid oxidase, and a solvent is an enzyme diluent. The enzyme diluent is used as a solvent, so that the whole blood treatment agent maintains a stable environment in a sample treatment process, and related enzyme activity is maintained. Ammonium chloride promotes erythrocyte lysis to release hemoglobin; ascorbic acid oxidase can catalyze ascorbic acid to be oxidized into dehydroascorbic acid, and interference factors are reduced. By using the whole blood treatment agent, a tedious pretreatment step on a whole blood sample by using a sample treatment liquid is not needed, the directly collected whole blood sample can be used for subsequent detection after being mixed with the whole blood treatment agent, and the directly collected whole blood sample can be used for subsequent detection, so that the sample treatment is simplified, and the risk of introducing an error due to the sample treatment is reduced; a detection result accords with a clinical diagnosis result, and the accuracy is high.

The invention provides a lipoprotein subfraction triglyceride detection kit which is suitable for a blood fat subfraction detector. Belongs to the technical field of in vitro diagnosis, the lipoprotein subfraction triglyceride detection reagent provided by the invention comprises a diluent, and the diluent comprises a first preservative, a first density regulator and a first solvent; the density liquid comprises a second preservative, a second density regulator and a second solvent; the reaction reagent comprises a surfactant, magnesium chloride, 4-aminoantipyrine, 4-chlorophenol, lipoprotein esterase, glycerophosphate oxidase, glycerol kinase, peroxidase, ascorbic acid oxidase, a protective agent, a third preservative and a third solvent; the kit provided by the invention determines the content of the lipoprotein subfraction triglyceride through a VAP system for the first time, and has the advantages of high precision and high accuracy.

The invention discloses a gametophyte male sterility related gene OsPTDb and application thereof. The amino acid sequence of the OsPTDb gene is as shown in SEQ ID NO. 1; the OsPTDb gene encodes an ascorbic acid oxidase which is an essential gene for male gametophyte development, the insemination of the male gametophyte cannot be completed and gametophyte male sterility is generated due to the abnormal function of the gene, and the OsPTDb gene plays an important role in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The application provides a method for directly detecting lytic polysaccharide monooxygenase activity in a fermentation broth and identifying oxidation types based on secondary hydrolysis of a product, color development by coupling of a glycol oxidase and a horseradish peroxidase. The method comprises the following steps: (1) culturing a recombinant strain of lytic polysaccharide monooxygenase to obtain fermentation supernatant thereof; (2) performing an oxidative cleavage reaction on a sample to be tested and a polysaccharide substrate in the presence of an electron donor and copper ions to obtain a reaction solution containing cleavage products; (3) adding ascorbate oxidase to the reaction solution to terminate the cleavage reaction of the lytic polysaccharide monooxygenase; (4) adding a glycosidase to the supernatant containing the cleavage products to perform secondary hydrolysis to obtain the sample to be tested; (5) adding a GOOX-HRP color development solution and the sample to be tested in a 96-well plate, and performing kinetic detection by using an enzyme-labeled instrument to measure the absorbance value at 515 nm; and (6) quantifying and classifying the sample to be tested according to the absorbance value. T. reesei ​

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.