3 results about "Ascorbate Oxidase" patented technology

The application provides an electrochemical uric acid test paper and a uric acid detection method. The electrochemical uric acid test paper has simple structure, low cost, easy manufacturing process, rapid reaction, sensitive detection, wide storage temperature, wide use temperature, wide effective period, accurate result, simple operation, etc. The uric acid detection method distinguishes the test current of ascorbic acid by different time and different excitation signals, subtracts the current signal of ascorbic acid from the current signal of uric acid and ascorbic acid, eliminates the interference of ascorbic acid, is simple to operate and low in cost, does not need to add other material components, does not need the participation of uric acid oxidase and ascorbic acid oxidase, directly distinguishes the current signal by different voltages, is short in test time, reduces the complex data correction process, maintains good repeatability of data, is simple in logic operation, basically has no influence on the repeatability of test signals, and can effectively remove the interference of ascorbic acid.

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.