5 results about "Ascorbate Oxidase" patented technology

The application belongs to the technical field of nanomaterials, and provides ascorbic acid oxidation mimetic enzyme and preparation thereof. The application adopts a methanol solution of zinc nitrate, copper acetylacetonate and 2-methyl imidazole to perform a hydrothermal reaction, and obtains the ascorbic acid oxidation mimetic enzyme by oxygen isolation calcination of a hydrothermal product. The copper-nitrogen-carbon nanomaterial obtained by the application has a rhombohedron structure, can specifically oxidize ascorbic acid, and can be applied to ascorbic acid content detection, oxidized ascorbic acid preparation and dehydroascorbic acid preparation as ascorbic acid oxidation enzyme mimetic enzyme. The nanomaterial can maintain ascorbic acid oxidation mimetic enzyme activity under acid, alkaline and high temperature conditions; the steady-state kinetic parameter (Michaelis constant is 0.012 mM) is better than that of natural ascorbic acid oxidation enzyme, and the ascorbic acid oxidation mimetic enzyme activity of the material has a broad application prospect in the fields of nanozyme, nanotechnology and biological analysis detection.

The application provides an electrochemical uric acid test paper and a uric acid detection method. The electrochemical uric acid test paper has simple structure, low cost, easy manufacturing process, rapid reaction, sensitive detection, wide storage temperature, wide use temperature, wide effective period, accurate result, simple operation, etc. The uric acid detection method distinguishes the test current of ascorbic acid by different time and different excitation signals, subtracts the current signal of ascorbic acid from the current signal of uric acid and ascorbic acid, eliminates the interference of ascorbic acid, is simple to operate and low in cost, does not need to add other material components, does not need the participation of uric acid oxidase and ascorbic acid oxidase, directly distinguishes the current signal by different voltages, is short in test time, reduces the complex data correction process, maintains good repeatability of data, is simple in logic operation, basically has no influence on the repeatability of test signals, and can effectively remove the interference of ascorbic acid.

The invention provides a copper calcium phosphate nano-enzyme fibroin antibacterial dressing as well as a preparation method and application thereof, and belongs to the technical field of material preparation. CCP HNS nano-enzyme with ascorbic acid oxidase is prepared through a coprecipitation method, nano-enzyme particles with controllable morphology, stable structure and excellent catalytic performance are obtained by regulating and controlling the ratio of copper to calcium and reaction conditions, and then the nano-enzyme particles are uniformly loaded on a natural protein material silk fibroin (SF), so that the nano-enzyme is prepared. The calcium copper phosphate nano-enzyme fibroin antibacterial dressing is obtained; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has an excellent antibacterial effect, is relatively low in cytotoxicity, maintains good cell viability and shows good biocompatibility; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has obvious advantages in the aspects of infection control, inflammation relief and tissue regeneration, can greatly shorten the wound closing time, shows good healing promoting ability, and has good practicability.

The invention provides a whole blood treatment agent, a kit and application of the whole blood treatment agent in detection of calprotectin, and relates to the technical field of in-vitro detection, the whole blood treatment agent comprises 5-15 mmoL / ml of ammonium chloride or 0.01-0.05 mg / ml of ascorbic acid oxidase, and a solvent is an enzyme diluent. The enzyme diluent is used as a solvent, so that the whole blood treatment agent maintains a stable environment in a sample treatment process, and related enzyme activity is maintained. Ammonium chloride promotes erythrocyte lysis to release hemoglobin; ascorbic acid oxidase can catalyze ascorbic acid to be oxidized into dehydroascorbic acid, and interference factors are reduced. By using the whole blood treatment agent, a tedious pretreatment step on a whole blood sample by using a sample treatment liquid is not needed, the directly collected whole blood sample can be used for subsequent detection after being mixed with the whole blood treatment agent, and the directly collected whole blood sample can be used for subsequent detection, so that the sample treatment is simplified, and the risk of introducing an error due to the sample treatment is reduced; a detection result accords with a clinical diagnosis result, and the accuracy is high.

This invention discloses a method for detecting organophosphorus pesticides using a self-cascaded Pt@Fe-CDs nanozyme, belonging to the field of chemical analysis and detection technology. This method synthesizes Pt@Fe-CDs nanozymes using iron-doped carbon dots (Fe-CDs) as a reducing agent and template. Pt@Fe-CDs simultaneously possess ascorbic acid oxidase-like and peroxidase-like activities. Under the catalysis of alkaline phosphatase, they hydrolyze L-ascorbic acid-2-phosphate trisodium salt to AA. Based on the fact that organophosphorus pesticides can inhibit ALP activity and hinder AA2P dephosphorylation, leading to a reduction in AA and thus causing changes in fluorescence and colorimetric signals, a simple and sensitive dual-signal fluorescence / colorimetric sensing platform for organophosphorus pesticides is constructed, with a detection limit of 0.28-0.53 ng / mL. The colorimetric and fluorescence biosensors established in this invention exhibit excellent performance in terms of sensitivity, specificity, and accuracy in actual samples.