Stable NEFA (non-esterified fatty acid) measuring kit

Abstract

The invention relates to a stable kit for measuring NEFA (non-esterified fatty acid) through an enzymic method. The kit comprises two separate liquid reagents, namely, a reagent 1 and a reagent 2 which are respectively placed, and is good in stability and anti-interference performance. The enzymic method is adopted to measure NEFA, and the method is easy, convenient and easy to operate. According to the kit, novel chromogen is selected, and anti-interference agents such as ascorbic acid oxidase and potassium ferrocyanide are added into the formula at the same time, so that the reagent has the characteristics of being high in sensitivity and anti-interference performance; a liquid stabilization technology is adopted, and a series of special stabilizers, chelating agents and enzymatic protective agents are added, so that the reagent is good in stability, and can be widely applied to most semi/full automatic biochemical analyzers, and used for monitoring human body fat metabolic status, lipid level, and the like, and clinical popularization is facilitated.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a stable free fatty acid assay kit. Background technique [0002] Free fatty acids, namely non-esterfied fatty acids (NEFA), are intermediate products of fat metabolism in the human body, substrates of cell membrane lipid structures and donors of intracellular signaling molecules such as prostaglandins. When glycogen, the energy source for muscle activity, is exhausted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Although free fatty acids only account for a small part of body fat, they meet a large part of energy demand, so they are important indicators for monitoring body fat metabolism and glucose metabolism. Free fatty acids can not only reflect the body's fat metabolism and blood lipid levels, evaluate blood sugar and assist in the diagnosis of diabetes, but also reflect a variety of other pathological and physiological...

AI Technical Summary

Problems solved by technology

[0011] 1. The kit for the determination of free fatty acid by enzymatic method was originally imported from abroad. The imported reagent has good stability and excellent analytical performance, but it is expensive and the detection cost is high, so it is not suitable for large-scale application

Nowadays, the biochemical detection of free fatty acids is in the ascendant in the domestic market, and only a few manufacturers can realize the self-production of free fatty acid kits, but the chromogen reaction substances used in them have low activity, poor color stability and weak anti-interference , resulting in poor stability of the entire kit, inaccurate detection results, and low sensitivity

[0012] 2. Since the peroxidase that catalyzes the Trinder reaction has poor substrate specificity, the reaction is easily interfered by endogenous substances, such as ascorbic acid, bilirubin, hemolysis, etc.

Reducing substances such as ascorbic acid will also react with H 2 o 2 Compete with peroxidase (POD) or compete with chromogenic substances for H 2 o 2 , so that the H produced during the oxidation process 2 o 2 is consumed, and the red quinone imine compound produced decreases, resulting in inaccurate determination results

[0013] 3. At present, the commercially available enzymatic free fatty acid kits all use a single stabilizer. Since the free fatty acid kit contains a variety of enzymes, the stability of the kit is directly related to the enzyme activity, and the activity of the enzyme is easily affected by various enzymes. The interference of factors, such as pH, temperature, ultraviolet rays, heavy metal salts, inhibitors, activators, etc., lead to the lack of stability of the kit and short storage time, which affects clinical use

A single stabilizer can no longer meet the requirements for the stability of the kit. Therefore, two or more stabilizers with simple preparation, low cost, and good stabilizing effect on free fatty acid assay kits with many types of enzymes should be selected in combination. agent is very necessary

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