71results about How to "Meet the needs of clinical applications" patented technology

The invention discloses a mussel mucoprotein gel for repairing and reliving itching. The mussel mucoprotein gel is prepared from the following components in percentage by weight: 0.15%-0.2% of mussel mucoprotein, 0.5%-2% of a macromolecule host material, 3%-10% of a humectant, 1%-5% of a surfactant, 0.1%-0.5% of a preservative, 1%-2% of a protein protectant, 0.5%-3% of a stabilizer, 0.5%-1% of a bacteriostatic agent, 0.8%-5.2% of a pH modifier and the balance of purified water. The mussel mucoprotein gel has good biological adhesion, is rapidly and effectively adhered to a wound surface, is capable of rapidly reliving itching and easing pain, and has the advantages of being low in immunogenicity, good in biocompatibility with a human body, convenient to use, free of thrill to skin, safe, nontoxic and free of bad reaction.

The invention discloses a method for preparing a polyvinylidene fluoride affinity membrane using amino acid as a ligand. The affinity membrane is prepared by hydrophilically modifying a polyvinylidene fluoride hollow fiber membrane and grafting the amino acid ligand. The grafting amount of the amino acid is 150 to 250mg/g for each membrane. The invention also discloses application of the affinity membrane in removing endotoxin in blood plasma, which removes the endotoxin in the blood plasma by a dynamic absorption means. The polyvinylidene fluoride affinity membrane prepared by the method has stable performance, good bio-compatibility and high endotoxin removing efficiency, and can be used for whole blood perfusion as well as blood plasma perfusion.

The invention relates to a dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and a preparation method thereof. The preparation method comprises the following steps: dissolving CTAB, and adding NaOH; dropwise adding TEOS; stirring, aging, washing, drying and roasting; dispersing a product in a toluene solution, adding APTES, refluxing, washing, dispersing through water to obtain a solution a, adding a chelating agent in DMSO, dissolving, separately adding NHS and EDC, stirring, adding the solution a, adding a gadolinium salt, filtering, washing, and dispersing with water to obtain a solution b; adding fluorescent molecules containing carboxyl in another DMSO, separately adding NHS and EDC, stirring, adding the solution b, stirring, filtering, washing, drying to obtain the dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica. The difunctional contrast agent has the advantages of small particle size, stable performance, good biocompatibility and strong fluorescence and magnetic resonance signals.

The invention provides a method for preparing a nano-silver-based dual-curing dental antibacterial adhesive. The method comprises the following specific steps: (1) preparing monodisperse spherical nano SiO2 particles by using a Stober method; (2) generating nano silver particles with small particle sizes on the surfaces of the nano SiO2 particles by utilizing a reduction reaction of an ammonia silver nitrate solution, namely preparing a nanoscale silver-loaded inorganic antibacterial agent; (3) performing proper surface modification on the previous silver-loaded inorganic antibacterial agent; and (4) preparing the optical and thermal dual-curing dental adhesive, and adding the inorganic antibacterial agent. The dental adhesive prepared by the invention has the multiple advantages of simple and feasible preparation process, product system stability, high operability, excellent antibacterial performance, durability and the like and has high clinical application potential.

The present invention provides a strain, which is a Lactobacillus gasseri strain and is characterized in that the preservation number is CGMCC No.6362. The present invention further provides uses of the strain in preparation of drugs for prevention and/or treatment of vaginitis, preparation of vagina care products, and preparation of external genitalia sanitary products. With the technical scheme, antagonism effects of the Lactobacillus gasseri strain on multiple pathogenic bacteria can be achieved. In addition, the strain has high adhesion to vaginal epithelial cells. The strain can well meet practical clinical application requirements.

The invention discloses a preparation method of an ultrasound and magnetic resonance two-mode contrast medium having lymph targeting. The preparation method comprises the following steps: adding a surfactant to an alkaline solution, stirring, adding a silicon source to the solution after the surfactant is dissolved completely, continuously stirring, adding a magnetic compound to generate a sol mixture, stirring, washing and carrying out centrifugal drying to obtain a powdery product; carrying out high-temperature calcination on the product to obtain powdery nanometer mesoporous silica particles without the surfactant; dispersing the particles in an organic solvent, adding a silane coupling agent, stirring, washing with ethyl alcohol and water, and carrying out centrifugal drying to obtain a powdery product; dispersing the powder in deionized water, adding a cross-linking agent and sodium hyaluronate, stirring, washing with water and carrying out centrifugal drying to obtain nano-particles; and dispersing the particles in deionized water, dropwise adding fluorocarbon, stirring, washing with water and drying to obtain the contrast medium. By utilizing the preparation method, integration of double imaging modes can be realized, and two imaging manners can be developed.

The invention provides a preparation method of an emulsion for magnetic resonance imaging of lung. The preparation method of the emulsion for magnetic resonance imaging of the lung comprises the following specific steps: (1) adding a certain amount of magnevist solution into water, stirring and adding dipalmitoyl phosphatidyl choline, mPEG (polyethylene glycol)-DSPE (distearoyl phosphatidyl ethanolamine), phospholipid, surfactant, co-surfactant and stabilizer; (2) magnetically stirring at the room temperature till no obvious blocky substance is produced to obtain an emulsion; (3) adding pulmonary alveoli spreading agent into the emulsion and further stirring to obtain a mixed solution; (4) intermittently stirring the mixed solution by using a homogenizer, then standing, centrifuging and removing sediments to obtain an emulsion containing magnevist. The prepared emulsion for magnetic resonance imaging of lung is capable of entering the body through a pulmonary drug delivery way, avoiding a large quantity of inconveniences brought by parenteral drug delivery and meeting the requirements on a variety of clinical applications.

The invention discloses a multifunctional probe and a preparation method and application thereof. The nano diagnosis and treatment probe with the two functions of magnetic resonance imaging and thermal therapy is formed in the mode that nano gold bars are formed by adding surfactant, chloroauric acid, a reduction agent and silver nitrate, target macromolecules are inoculated, and gadolinium ions are adsorbed. The preparation method is simple, the probe is good in dispersity in an aqueous solution, good in stability and capable of meeting requirements of clinical application and combining radiography and thermal therapy, and the dual functions of lymphoma diagnosis and treatment are achieved.

The invention provides a preparation method for a porous calcium phosphate stent. The preparation method comprises the following steps: with polycaprolactone (PCL) as a printing template, dissolving polycaprolactone in acetone so as to obtain a polycaprolactone solution, and uniformly mixing a mixture of tetracalcium phosphate (TTCP) and calcium hydrogen phosphate (DCPD) with the above polycaprolactone solution to prepare printing slurry; subjecting the slurry to 3D printing to print a green stent body with a certain shape and of a certain structure, and placing the stent in a low-temperaturehydration environment for a reaction so as to form a hydroxyapatite HAp porous stent, thereby avoiding dimensional shrinkage deformation caused by high-temperature sintering and reducing energy loss;and finally, putting the porous stent in acetone to dissolve and remove the stent template PCL so as to obtain the individually-controllable porous calcium phosphate stent with HAp as a main componentand with certain osteoinductivity and mechanical strength.

The invention provides a high-purity minicircle DNA (deoxyribonucleic acid) and a preparation method and application thereof. The preparation method includes the steps: 1) providing a parental plasmid containing a target sequence, wherein the parental plasmid has a specific recombination site, a nucleotide sequence of a skeleton DNA and a nucleotide sequence of the minicircle DNA; 2) transferring the parental plasmid to a host cell, inducing the parental plasmid to generate the minicircle DNA and the skeleton DNA containing the target sequence under the action of site-specific recombination; 3) subjecting the host cell to lysis, and subjecting plasmids to prepurification to obtain mixed plasmids including the minicircle DNA, the parental plasmid containing the target sequence and/or the skeleton DNA containing the target sequence; 4) removing the parental plasmid containing the target sequence and/or the skeleton DNA containing the target sequence from the mixed plasmids according to a tri-spiral purification method to obtain the high-purity minicircle DNA.

The invention discloses a porous metal bone implant material and its preparation method and application and belongs to the technical field of biomedical materials. The porous metal bone implant material comprises a porous metal material matrix and a silk fibroin material filling the pores of the porous metal material matrix. The porous metal material matrix is prepared from metal raw material powder through 3D printing. The silk fibroin material is loaded with a bioactive drug. The preparation method comprises 1) preparing a customized 3D printed porous metal material matrix according to demands, 2) preparing a silk fibroin solution and loading the silk fibroin with a bioactive drug, and 3) filling pores of the porous metal material matrix with the silk fibroin with the bioactive drug, and carrying out freeze-drying to obtain the porous metal bone implant material. The porous metal bone implant material is precisely controlled in structure, can be customized, can effectively improve osseointegration of a metal bone implant in diabetic patients and can reduce the loosening rate of the implant.

The invention relates to a contrast agent having two functions of up-conversion luminescence and magnetic resonance imaging, and a method for preparing the same. The contrast agent is obtained by modifying a gadolinium chelating agent on the surfaces of carbon nanospheres through taking up-conversion luminescent material carbon nanospheres as cores and using a coupling agent, wherein the mol ratio of the carbon nanospheres to the coupling agent to the gadolinium chelating agent is 1: 1: 1 to10: 1: 1. The dual-function contrast agent is small in grain size, high in crystallinity, even in dispersion, stable in performance, and strong in both fluorescence and magnetic resonance signal. The product obtained is capable of meeting the requirements of clinical application.

The invention discloses an immunofluorescence kit for quantitatively detecting the content of troponin I and a preparation method. The preparation method comprises the following steps: preparing a troponin antibody-large-particle fluorescent particle conjugate; preparing a nitrocellulose membrane labeled antibody; assembling a reagent card; preparing a TNI calibration product; establishing a standard curve; calculating the content of the troponin in a sample to be detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I comprises a nitrocellulose membrane, a reagent and the calibration product. The lowest detection limit of the kit can reach 0.1ng/ml and the requirement of clinical application is met; the fastest time of a process from sample acquisition to detection result presentation only needs 10 to 15 minutes so that more expensive time for patients is provided, and illness states of the patients can be dynamically detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I has good result relevance, no remarkable difference and an accurate and reliable detection result, and can be used for clinically replacing imported products; the detection cost is greatly reduced.

The invention relates to a preparation method of a sandwich type degradable stent for alveolar bone repair as well as a product and application thereof. The preparation method comprises the following steps: preparing a self-curing hydroxyapatite and PLGA double-layer stent through double-nozzle 3D printing; and afterwards, performing amination of the surface of the polyester, and chemically fixing the silk fibroin coating loaded with the endothelial growth promoting medicine through an EDC/NHS coupling reaction. The hydroxyapatite stent matched with bone trauma is prepared, the bioactive coating modified PLGA provides a matched base for external fixation of bone wounds, the hydroxyapatite stent is used in cooperation with bone glue, bone nails and suture lines to achieve the fixing effect, and accurate bone grafting is achieved. All the materials are biodegradable, the degradation rate of the materials is suitable for growth of new bones, the silk fibroin coating loaded with VEGF improves the compatibility of PLGA and mucous membranes, the defects that a traditional titanium mesh stent is exposed outside the mucous membranes in advance and cannot be degraded in vivo are overcome, and the requirements of clinical application are met.

The invention relates to a preparation method of a lymphatic targeted molybdenum sulfide nanosheet and a product thereof. The molybdenum sulfide is synthesized by means of a hydrothermal synthetic method, and sulfide nanoparticles are embedded at the same time, so that the spaces among layers are enlarged, and peeling is facilitated further. By taking an organic solvent as a reaction solvent, molybdenum sulfide is peeled by a liquid phase to prepare the two-dimensional molybdenum sulfide nanosheet. Finally, modified by hyaluronic acid, the lymphatic targeted two-dimensional molybdenum sulfidenanosheet is prepared. The invention relates to the novel method of preparing molybdenum sulfide. The method, by being applied to treating cancers clinically, can achieve targeted photothermal therapyof tumor cells and prevent normal cells from being killed.

The invention relates to a preparation method of a detection probe for alpha-synuclein accumulation as well as a product and application of the detection probe. According to the preparation method, magnetic ferroferric oxide nanoparticles (Fe3O4 NPs) are used as carriers, and tetraethyl orthosilicate (TEOS) and 3-aminopropyltrimethoxysilane (APTES) are used for aminated modification of the surfaceof Fe3O4 NPs; further, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) are used as coupling agents for binding alpha-synuclein monoclonal antibodies; the Fe3O4 NPscarriers are coated with silica shell by utilizing the autocatalytic activity of Fe3O4 NPs, and then the alpha-synuclein monoclonal antibodies are bound to synthesize the detection probe which targets a Parkinson's disease, and is capable of fixed-point clearing intracellular ROS, and delaying the further accumulation of alpha-synuclein. The detection probe combines targeting, imaging and treatment into one, and is simple in synthesis method; the raw materials used are high in biosafety, relatively good in catalytic activity and good in physical stability.

The invention relates to a fast propagation method for cultivating a salt-tolerant and low temperature-tolerant periwinkle strain. The fast propagation method disclosed by the invention is based on preparation of a periwinkle growth culture medium, induction of the growth culture medium and screening of the culture medium in a salt-tolerant and low temperature-tolerant manner, wild periwinkle blades are taken as original materials for inducing an aseptic seedling, and a salt-tolerant and low temperature-tolerant periwinkle strain cultivation system is established by fast propagation and multi-generation induction acclimatization of the aseptic seedling, wherein the alkaloid content of the salt-tolerant and low temperature-tolerant periwinkle strain is higher than that of wild periwinkle by about 20%. The fast propagation method disclosed by the invention has the advantages that, (1) the cultivation process is controllable completely and can not affected by a natural environment; (2) the salt-tolerant and low temperature-tolerant periwinkle can grow normally on a saline-alkali mudflat; and (3) the growth period of the periwinkle in the Yangtze River valley is prolonged and the biomass of the periwinkle is increased.

The invention discloses a method for obtaining maternal mesenchymal stem cells from placenta. The method for obtaining the maternal mesenchymal stem cells from the placenta comprises the following steps: obtaining placenta tissues from a position, which are within 5cm away from the radius of an umbilical cord and are 0.5 cm away from a fetal surface, of the placenta; and carrying out enzyme digestion treatment on placenta tissues by adopting an enzyme digestion solution, and then carrying out cell culture on a product of the enzyme digestion treatment to obtain the maternal mesenchymal stem cells. According to the method disclosed by the invention, the technical problem of separating the maternal mesenchymal stem cells from the placenta is solved, the prepared maternal mesenchymal stem cells are large in quantity and high in purity, neonatal cells are not easy to mix, and the clinical application requirements can be met; the method provided by the invention solves the problems that thequantity of the maternal mesenchymal stem cells obtained from the decidua basalis is small and the decidua basalis is easy to lose, and provides a new scheme and way for obtaining the maternal mesenchymal stem cells from the placenta.

The invention relates to preparation of polyester/periodic mesoporous bone-filling composite material with a fluorescently-labeled degradation rate, a product and application. The preparation comprises the steps of firstly, synthesizing rare-earth periodic mesoporous nanoparticles with fluorescence performance, then, subjecting the rare-earth periodic mesoporous nanoparticles to chemical modification by taking poly-L-lactic acid with low molecular weight as a modifier, and finally, adding the modified nanoparticles into a polyester matrix by a solution blending method. The invention further provides the product obtained by the method and application of the product in bone restoration as a composite bone filling material. The fluorescent mesoporous material obtained by the method provided by the invention is uniformly dispersed in an organic matrix, mechanical properties of a polyester are improved greatly, and the degradation behavior of the composite bone filling material can be monitored in real time. The preparation process is simple, and the composite bone filling material can serve as a bone filling material of load bearing bone parts with relatively high performance index requirements. The obtained composite material can meet the requirements of clinical application.