Fast propagation method for cultivating salt-tolerant and low temperature-tolerant periwinkle strain

A periwinkle, low temperature-resistant technology, applied in the field of bioengineering, can solve problems such as unsatisfactory chemical synthesis, and achieve the effect of increasing biomass and prolonging growth period

Inactive Publication Date: 2012-08-01
上海安体康生植物化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The content of vinblastine and vincristine in plants is extre

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1:

[0016] This embodiment includes the following steps:

[0017] 1) Preparation of Catharanthus roseus growth medium, induced growth media and salt-tolerant and low-temperature-tolerant screening media: Take MS basic medium and add 6 grams of agar powder per liter to obtain Catharanthus roseus growth medium for use; take MS basic Medium, add 0.1 g of naphthalene acetic acid, 3 g of 6-benzylaminopurine, 0.2 g of colchicine and 6 g of agar powder per liter as a unit to obtain an induction growth medium for use; another MS basic medium is used per liter Add 10 grams of sodium chloride and 6 grams of agar powder as a unit to obtain a salt-tolerant low-temperature screening medium for use.

[0018] Adjust the pH value of the growth medium: use a pH meter to measure the pH value of the solid growth medium and the liquid growth medium, and use HCl and NaOH to adjust the pH value of the Catharanthus roseus growth medium, the induced growth medium and the salt-tolerant low-t...

Example Embodiment

[0023] Example 2:

[0024] This embodiment includes the following steps.

[0025] 1) Preparation of Catharanthus roseus growth medium, induced growth media and salt-tolerant and low-temperature-resistant screening media: Take MS basic medium and add 6.5 grams of agar powder per liter to obtain Catharanthus roseus growth medium for use; take MS basic Medium, add 0.15 grams of naphthaleneacetic acid, 2 grams of 6-benzylaminopurine, 0.3 grams of colchicine and 6.5 grams of agar powder per liter as a unit to obtain an induction growth medium for use; another MS basic medium is used per liter Add 15 grams of sodium chloride and 6.5 grams of agar powder as a unit to obtain a salt-tolerant low-temperature screening medium for use.

[0026] Adjust the pH value of the growth medium: use a pH meter to measure the pH value of the solid growth medium and the liquid growth medium, and use HCl and NaOH to adjust the pH value of the Catharanthus roseus growth medium, the induced growth medium and ...

Example Embodiment

[0031] Example 3:

[0032] This embodiment includes the following steps.

[0033] 1) Prepare Catharanthus roseus growth medium, induced growth media and salt-tolerant and low-temperature resistant screening media: Take MS basic medium and add 7g of agar powder per liter to obtain Catharanthus roseus growth medium for use; take MS basic Medium, add 0.2 g of naphthalene acetic acid, 3.5 g of 6-benzylaminopurine, 0.3 g of colchicine and 7 g of agar powder per liter as a unit to obtain an induction growth medium for use; another MS basic medium is used per liter Add 15 grams of sodium chloride and 7 grams of agar powder as a unit to obtain a salt-tolerant low-temperature screening medium for use.

[0034] Adjust the pH value of the growth medium: use a pH meter to measure the pH value of the solid growth medium and the liquid growth medium, and use HCl and NaOH to adjust the pH value of the Catharanthus roseus growth medium, the induced growth medium and the salt-tolerant low-temperatur...

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Abstract

The invention relates to a fast propagation method for cultivating a salt-tolerant and low temperature-tolerant periwinkle strain. The fast propagation method disclosed by the invention is based on preparation of a periwinkle growth culture medium, induction of the growth culture medium and screening of the culture medium in a salt-tolerant and low temperature-tolerant manner, wild periwinkle blades are taken as original materials for inducing an aseptic seedling, and a salt-tolerant and low temperature-tolerant periwinkle strain cultivation system is established by fast propagation and multi-generation induction acclimatization of the aseptic seedling, wherein the alkaloid content of the salt-tolerant and low temperature-tolerant periwinkle strain is higher than that of wild periwinkle by about 20%. The fast propagation method disclosed by the invention has the advantages that, (1) the cultivation process is controllable completely and can not affected by a natural environment; (2) the salt-tolerant and low temperature-tolerant periwinkle can grow normally on a saline-alkali mudflat; and (3) the growth period of the periwinkle in the Yangtze River valley is prolonged and the biomass of the periwinkle is increased.

Description

technical field [0001] The invention relates to a cultivation method of periwinkle, in particular discloses a method for rapid propagation of a salt-tolerant and low-temperature resistant periwinkle strain, and belongs to the technical field of bioengineering. Background technique [0002] Vinca (Catharanthus roseus) is a plant of Apocynaceae, which contains more than 100 kinds of indole alkaloids, most of which have biological activity. The alkaloids extracted or semi-synthesized from Vinca include vinblastine sulfate (vinblastine), vincristine sulfate (vincristine), vindesine sulfate (vinblastine, Vindestine, VDS), vinorelbine tartrate (Vinrelbine, VNB), is At present, the most widely used natural plant anti-tumor drug, there are two vinblastine analogues (anhydrovinblastine anhydrovinblastine and 20,20'-difluorovinorelbine, vinflunine) are under clinical research. The research and development of periwinkle is also one of the most potential and active fields at present. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 倪迪安倪新忠
Owner 上海安体康生植物化学有限公司
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