206 results about "Catharanthus roseus" patented technology

The present invention provides formulas for producing compositions for the structural / functional nutritional support for those who struggle with poor focus, concentration and / or memory. In addition, the present invention provides compositions comprising nutritional / botanical factors helpful to those who subjectively experience transient mental fatigue or poor cognitive function. The compositions of this invention consist primarily of the following ingredients B-complex vitamins, antioxidants, minerals, phosphatidyl serine (PS), choline, dimenthyl-aminoethanol (DMAE), docosahexaenoic acid (DHA), L-pyroglutamic acid, as well as herbal extracts from Bacopa monniera, Vinca minor, and Huperzia serrata. The present invention also relates to the administration of these compounds to alleviate mental fatigue or poor cognitive function.

The invention relates to an antifungal composition for the treatment of human nails containing extracts of walnut hull, pulverized roots of Nardostachys jatamansi or Vetiveria zizanioides or Catharanthus roseus, polyols, fixed oil, non-ionic emulsifiers, thickening agent plasticizer and base. The invention also relates to a process for the preparation of the above synergistic composition.

The invention discloses an induction and in-vitro culture method of plant stem cells derived from apical meristem of catharanthus roseus roots. The induction method comprises the following steps: cutting off a root cap from the root tip of the catharanthus roseus, performing inoculating to an induction culture medium, performing culturing in dark, and proliferating and growing a stem cell layer inan explant to form a cell cluster; transferring the layered stem cell layer to a subculture medium for culturing for 10-15 days, and performing complete culturing in dark; and performing transferringto a new subculture medium, and performing culturing for 10-15 days under the same condition to obtain a catharanthus roseus stem cell line with stable cell morphology. A catharanthus roseus stem cell culture system is established by inducing apical meristem of the catharanthus roseus roots, stable subculture can be performed under certain conditions, and epigenetic variation is avoided. The catharanthus roseus stem cell culture system established by the invention has the characteristics of being high in growth speed, high in genetic stability and the like, and culture with a 10L bioreactor is preliminarily realized.

The present invention provides a novel nicotine free anticigarette herbal formulation as an anti-dote to the poisoning effects of tobacco products such as Cigarettes, Gutka, Pan masala and other similar tobacco related products. Formulation(s) comprises of sterilized dried plant powder / extracts together with the conventional additives to form the oral dosage forms, which include tablets, capsules, syrup and powders ready for suspension and mouth spray. The anti tobacco addiction herbal formulation comprises of Sesbania grandiflora, Catharanthus roseus, Ocimum sanctum, Myristica fragrans, Elettaria cardamomum, Carum copticum, Syzgium aromaticum, Cinnamomum tamala, Acorus calamus, Zingiber officinale, Piper nigrum, Cinnamomum zeylanicum, Cuminum cyminum, Nigella sativum, Cinnamomum camphora, Piper longum Ocimum gratssimum and Hemidesmus indicus.

The invention provides intravenous nanomicelle agents of vinca alkaloids antitumor drug, it contains an effective dose for treating of vinca alkaloids antitumor drug (vinblastine and vincristine or vindesine), macrogol derivatization phospholipid, and the pharmaceutical acceptable adjuvants. Its preparation is to pack the drug in the formative nanomicelle agents, prepare and make the intravenous nanomicelle agents of vinca alkaloids antitumor drug. Vinca alkaloids antitumor drug and macrogol derivatization phospholipid form into a very uniform size nanomicelle. In micelles, polyethylene glycol molecule and hydrophobic core for drug packing form a hydrophilicitious inhibitory coating, avoid the drugs contact with the protein such as enzymes in the blood and identified and phagocytized by the endothelial system in vivo, phagocytosis, the cycle time of micellar in vivo is extended. In addition, the micellar drug also increases the storage stability and the effect on the tumor of the drug and reduces drug toxicity.

The invention belongs to the plant protection subject of the agricultural category, and particularly relates to citrus yellow shoot candidatus liberibacter asiaticus detection test paper. The detection test paper is prepared through the following steps that pathogenic bacteria antigen is extracted and purifed from catharanthus roseus host with citrus yellow shoot candidatus liberibacter asiaticus antigen and used for injecting immunized rabbits to obtain yellow shoot candidatus liberibacter asiaticus specific antiserum, the yellow shoot candidatus liberibacter asiaticus specific antiserum is purified to obtain the citrus yellow shoot candidatus liberibacter asiaticus antigen, immune colloidal gold is prepared, a combined pad which is soaked with the immune colloidal gold and a chromatography membrane which is coated with a detection line T-citrus yellow shoot candidatus liberibacter asiaticus antigen and a quality control line C-goat anti-rabbit antibody are further prepared, and finally a detection test paper product is assembled. Against for the requirement of the detection of a great amount of citrus yellow shoot samples, the citrus yellow shoot candidatus liberibacter asiaticus detection test paper which is convenient, rapid and high in specificity is provided. The test paper has following advantages that 1, convenience in use and simpleness in operation are realized; 2, a detection result can be obtained within a short period of time; 3, the stability is good; and 4, the cost is low, and the test paper is particularly suitable for the discrimination of citrus yellow shoot plants of a fruit garden.

The invention discloses a method for constructing close-to-nature vegetations in a southern coastal sandy land. The method disclosed by the invention comprises the following steps: firstly, screening six plants, namely lpomoea pes-caprae, scaevola taccada, spinifex, vitex rotundifolia, catharanthus roseus and stachytarpheta jamaicensis, which are suitable for planting in the coastal sandy land, and forming two combinatorial planting; then, digging planting holes, adding family manure as a base fertilizer, adding a water-retaining agent, placing straws on the peripheries of the planting holes to form straw checkboards (which have the function of improving the microenvironment (light shading, wind keepout and the like) for seedling growth, and the straws can be used as a fertilizer after being rotted), soaking bag seedlings with water, adding a compound fertilizer, backfilling sand or foreign soil, and maintaining. After about two months, the six plants are relatively good in growth effect and can form the close-to-nature vegetations; in addition, the vegetations are relatively simple to manage and maintain, and the survival rate reaches over 90 percent respectively.

Formulas for producing compositions for the dietary supplement and nutraceutical products, containing virgin olive oil, vitamins, substances as vincamine, vinpocetine or vinburnine, as well as herbal extracts from Vinca Minor and Crioceras longiflorus. The present invention also relates to the oral administration of these compounds, to alleviate mental fatigue or poor cognitive function and anti-ageing prevention.

The invention discloses an LAMP primer group for detecting phytoplasma as well as a kit of the LAMP primer group and an application of the kit. The LAMP primer group, which is strong in specificity and is constituted by nucleotide sequences shown as SEQ ID No.2, SEQ ID No.3, SEQ ID No.4 and SEQ ID No.5, is finally obtained by designing six groups of primers for six regions of the conserved sequence of a 16S gene of 16SrI group phytoplasma and designing two groups of primers for six regions of the conserved sequence of a tuf gene of the 16SrI group phytoplasma. The invention further discloses the kit of paulownia witch phytoplasma, Chinaberry witch phytoplasma, mulberry dwarf phytoplasma, lettuce yellow phytoplasma and catharanthus roseus green phytoplasma prepared by virtue of the primer group and the invention also establishes a detection method; the detection method has the advantages of being good in stability, strong in specificity, high in sensitivity and the like; and the method is applicable to the detection of the paulownia witch phytoplasma, the Chinaberry witch phytoplasma, the mulberry dwarf phytoplasma, the lettuce yellow phytoplasma and the catharanthus roseus green phytoplasma.

The present invention provides therapeutic compositions derived from plants useful for treating diabetes. The present invention also provides methods for screening plant metabolites and constituents to identify therapeutic agents and / or dietary supplements for treating diabetes. The therapeutic compositions can be derived from plants such as Phyllanthus emblica, Azadiractha indica, Terminalia chebula, Mucunapuriens, Curcuma longa, Ficus glomerata, Phyllanthus niruri, Momordica charantia, Euphorbia royleana, Catharanthus roseus, Eugenia jambolana, Emblica officinalis, Gymnema sylvestre, Melia azadirechta, Morinda citrifolia, Pterocarpus marsupium, Tinospora cardifolia, Tribulus teristris, Trigonella foenum graecum, and Withania somnifera.

The invention discloses an appetizing beef cookie and a processing method of the appetizing beef cookie. The appetizing beef cookie is composed of, by weight, 20-30 parts of beef, 5-8 parts of corn flour, 80-100 parts of flour, 10-15 parts of cream, 8-10 parts of cocoa powder, 2-3 parts of fructus amomi, 2-4 parts of amomum tsao-ko, 1-3 parts of endothelium corneum gigeriae galli, 2-4 parts of hawthorns, 2-3 parts of poria cocos, 1-3 parts of madder, 2-4 parts of tulips, 2-3 parts of achyranthes bidentata, 1-3 parts of catharanthus roseus, 8-10 parts of malt sugar, and 8-10 parts of nutrition additives. The processing method is simple in operation, the formula is reasonable, the source of raw materials is wide, and the appetizing beef cookie processed according to the method is crispy, delicious and agreeable in sweetness; meanwhile, due to the fact that Chinese herbal medicinal ingredients such as fructus amomi, amomum tsao-ko and endothelium corneum gigeriae galli are added into the raw materials, the appetizing beef cookie has the health care effects of coordinating intestines and stomach and promoting appetite.

The invention discloses a method for increasing content of camptothecin in camptotheca hairy root in the technical field of biology. The method comprises the following steps of: cloning coding frame sequences of CrORCA3 and CrG10H genes from catharanthus roseus; constructing a plant bivalent high-efficiency expression vector including the genes; and genetically transforming the camptotheca into camptotheca hairy root genetically modified by CrORCA3 and CrG10H genes, by using the hair root agrobacterium-mediated technique. The camptothecin content in the obtained genetically-modified camptotheca hairy root is increased obviously. The content of the camptothecin is 5.4 times of the contrast. The content of hydroxycamptothecin is 2.3 times of the contract. The content of the hairy root camptothecin independently genetically-modified by CrORCA3 is increased by 1.5 times of the contract. The content of the hairy root camptothecin independently genetically-modified by CrG10H gene is not increased obviously. The invention provides a method for increasing content of the camptothecin in the camptotheca hairy root, thereby providing a novel method for producing the camptothecin and the hydroxycamptothecin as anti-cancer drugs of important clinical requirements.

The invention relates to a method to extract the vinblastine extracted from the catharanthus roseus, including the following processes: (1) the catharanthus roseus is agitated and smashed after the wetting by the acidic water and then is soaked in the acidic water, the filtrate after the filtration is regulated to alkalinity by using alkali, a alkaline water phase is extracted by using chloroform, a chloroform phase is collected, and the solid reclaimed materials can be obtained after the reclaiming of the chloroform; (2) the reclaimed materials are dissolved by using the acidic water, the ethyl acetate is added for extraction, and a acidic water phase is reclaimed; (3) the reclaimed acidic water phase in step (2) is regulated to alkalinity, the chloroform is used for extraction, and the crude alkaloid is obtained by the reclaim of chloroform of the extraction phase; (4) the obtained crude alkaloid in step (3) is dissolved by using the acidic water, of which the pH is regulated to alkalinity, the lower layer of sediments is collected centrifugally, the refined catharanthus roseus alkaloid is obtained after the drying of the sediments, and the vinblastine and leurocristine can be obtained by alumina column chromatography separation; (5) the filtrate which is obtained by centrifugation in step (4) is extracted by using chloroform, the catharanthine and the vindoline are obtained by vacuum concentration and macroporous resin separation. The method can improve the utilization ratio of catharanthus roseus.

The present invention is method of regenerating vinca hairy root plant in biotechnology. The method of the present invention includes cutting sections of vinca hairy root explant, setting on callus tissue culture medium and lighting culturing to form callus, inoculating to adventitious bud culture medium to form bud, further lighting culturing to form adventitious bud, cutting adventitious bud and transferring to rooting culture medium to produce adventitious root, further culturing of the regenerated complete plant onto fast propagation culture medium, selecting plant and transplanting to flower pot with transplanting culture medium to obtain normally growing vinca plant via domestication. The present invention screens out proper culture medium, and has vinca hairy root adventitious bud differentiating rate up to 80 %, adventitious bud rooting rate up to 100 % and regenerated plant transplanting survival rate up to 100 %.

The invention discloses a garden herbicide synergistic combination which contains two effective constituents, namely imazapic and glyphosate isopropylamine, wherein the weight ratio of the imazapic to the glyphosate isopropylamine is 1:(0.8-13). The garden herbicide synergistic combination can be developed into more general water aqua and other general dosage forms conveniently applied to garden plants. The combination with the two constituents has a synergistic interaction effect, so that the weed removal spectrum can be expanded, the prevention and removal effect is enhanced, and the pesticide hazard to garden trees is reduced. A test result shows that cuscuta chinensis lam, catharanthus roseus, cuscuta reflexa, cuscuta australis and the like can be effectively prevented and removed. The garden herbicide synergistic combination has the characteristics of high obvious dispersion performance, wide weed removal spectrum, high durability, convenience in use, safety against garden trees, safety to human and environment and the like.

The invention discloses spicy mutton sauce and a processing method thereof. The spicy mutton sauce is prepared from the following raw materials in parts by weight: 40-50 parts of lamb leg meat, 2-4 parts of sealwort, 2-4 parts of sea-buckthorn, 2-3 parts of Chinese wolfberry fruit, 1-3 parts of cape jasmine, 2-4 parts of ginseng leaves, 2-4 parts of catharanthus roseus, 1-3 parts of tulip bulb, 10-20 parts of shelled-melon seeds, 5-8 parts of soybean oil, 5-8 parts of cayenne pepper, 3-5 parts of chopped green onion, 5-6 parts of table salt, 2-3 parts of aginomoto, and 7-10 parts of nutritional additives. The processing method is simple to operate and simple and reasonable in formula; the spicy mutton sauce is nutritionally balanced; traditional Chinese medicine components having the effect of preventing cancers such as the sealwort, the sea-buckthorn and the cape jasmine are added to the raw materials, so that the spicy mutton sauce has the effect of preventing cancers and the characteristics of being delicious in taste, spicy, palatable and rich in nutrient.

The invention discloses an anticancer fragrance-increasing cigarette holder. A cigarette holder tube (2) and a suction nozzle (3) are of an integrated structure; one end of a cigarette insertion opening (1) is inserted into the outer end of the cigarette holder tube (2); both ends of the cigarette holder tube (2) are separated by a filtration net (4) to form a filtration region; and an anticancer fragrance-increasing composition for cigarettes is filled in the filtration region and contains the following components: 2 to 3 parts of taxus chinensis, 0.5 to 1 part of curcuma zedoary, 0.5 to 1 part of tears, 0.5 to 1 part of catharanthus roseus, 0.5 to 1 part of cephalotaxus fortunei, 0.5 to 1 part of disporum leschenaultianum, 0.5 to 1 part of rabdosia rubescens, 0.5 to 1 part of meadow saffron, 0.5 to 1 part of camptotheca acuminate, and 0.5 to 1 part of Java brucea. The anticancer fragrance-increasing cigarette holder facilitates the filling and exchange of the anticancer fragrance-increasing composition for cigarettes, so the harm from carcinogenic substances to human organs can be greatly reduced and the harm from smokes to the human bodies is reduced to the minimum; moreover, instead of damaging the original taste of the cigarette by simply absorbing a large amount of smoke, the anticancer fragrance-increasing cigarette holder makes the taste of the cigarettes more purer and easily accepted by adding a large quantity of plant spices.

The invention discloses a traditional Chinese medicine plaster for treating depression and insomnia of middle-aged and old people. The etiology and pathogenesis of depression and insomnia of middle-aged and old people mainly are poor meridian circulation. The traditional Chinese medicine plaster is prepared by the following steps: grinding cypress branches, edgeworthia chrysantha lindl, plagiopus oederi limpr, medical angiopteris rhizome, sensitive plant, catharanthus roseus, rhizoma acori graminei, spinach fruit, root of procumbent Indian mallow, thickstiped pollia root, intermediate peashrub root and smilax riparia into powder; taking a proper amount of water and a little of honey; and mixing into paste. The paste is applied to the corresponding acupuncture point. Through a clinical experiment, the total effective rate can be up to 90.0%, and is obviously superior to that of a control group; and the traditional Chinese medicine plaster is free of a side effect.

The invention discloses bone-strengthening medicinal liquor, and belongs to traditional Chinese medicine health care liquor. The medicinal liquor is prepared by infusing traditional Chinese medicines prepared according to the following traditional Chinese medicine formula in liquor; according to the traditional Chinese medicine formula, a main formula comprises: 60 g of twoleaf swertia herbs, 30 g of aristolochia mollissima, 20 g of radix cyathulae, 60 g of vinegar-processed radix clematidis, 60 g of woodlouse, 20 g of eucommia, 25 g of radix aconiti preparata, 25 g of prepared kusnezoff monkshood roots, 20 g of phryma leptostachya, 20 g of herba epimedii, 60 g of radix dipsaci, 60 g of rhizoma drynariae, 100 g of native copper, 60 g of caulis spatholobi, 30 g of frankincense, 10 g of pawpaw, 20 g of philippine flemingia roots, 15 g of white peony roots, 15 of centipede, 15 g of scorpion, 3 pieces of prepared nux vomica, 20 g of bombyx batryticatus, 10 g of cornua cervi pantotrichum, 1 pair of sea horses, 15 g of deer's sinew, 15 g of rhizoma corydalis, 10 g of dragon's blood, 20 g of long-noded pit viper, and 15 g of myrrh; an assistant formula comprises: 25 g of girald daphne bark, 30 g of scorpion, 15 g of uncaria, 15 g of rhizoma gastrodiae, 20 g of catharanthus roseus, 25 g of carpesium abrotanoides, 15 g of ramulus mori, 10 g of cassia twig, 35 g of radix puerariae, 60 g of rhizoma cibotii, and 10 g of semen brassicae. The bone-strengthening medicinal liquor of the invention has good curative effect, quick effect, high cure rate, low recurrence rate, and no side effect with respect to lumbar vertebra and cervical vertebra hyperplasia, hyperosteogeny, osteoporosis, rheumatoid arthritis, and the like.

The invention relates to a Chinese patent medicament for treating intractable hypertension, which comprises the following Chinese herbal medicaments in part by weight: 50 to 100 parts of seabuckthorn, 80 to 100 parts of wild thyme, 100 to 150 parts of madagascar periwinkle herb, 100 to 150 parts of ganoderma lucidum karst, 150 to 300 parts of black fungus, 80 to 100 parts of emblic root, 50 to 100 parts of salidroside, 50 to 100 parts of devilpepper root, 200 to 300 parts of kudzuvine root, 200 to 300 parts of hawthorn root and 200 to 300 parts of cassia seed. The medicaments are subjected to reasonable compatibility and are complementary in medicinal effects, so the Chinese patent medicament has good effect of reducing blood pressure, can address both symptoms and root causes, is suitable for patients who suffer from senile hypertension, juvenile hypertension, gestational hypertension, salt-sensitive hypertension and the like and particularly has the special and obvious curative effect on patients who suffer from refractory hypertension; and the Chinese patent medicament has the cure rate of 82.7 percent on the refractory hypertension, and has the total cure rate of 68.6 percent, total effectual rate (cure and effect producing) of 89.3 percent and the total effective rate of 100 percent on various kinds of hypertension, and the hypertension does not relapse after being cured.

The invention belongs to the technical field of bioengineering, and provides a method for improving camptothecin content in hairy roots of novel medicine source plant ophiorrhiza pumila of camptothecin. The method comprises the following steps of: cloning coder frame sequences of CrORCA3 and CrG10H genes from catharanthus roseus; constructing a bivalent efficient expression vector of a plant containing such genes; and carrying out genetic transformation to the ophiorrhiza pumila through the agrobacterium rhizogenes mediated transformation method, thus obtaining the hairy roots of the ophiorrhiza pumila containing transformed CrORCA3 and CrG10H genes. The camptothecin content in the obtained hairy roots of the transgenic ophiorrhiza pumila is obviously increased, the camptothecin output in each flask(2.62mg / flask) is averagely increased by 5.52 times in comparison with that in the contrast, and the average content (8.44mg / g DW) is increased by 4.04 times. The total camptothecin output of each flask, induced by plant hormones after culturing one strain for six weeks, is increased by 7.8 times. The preparation method provided by the invention is a novel method for producing an anti-cancer drug that is camptothecin under important clinical demand.

The invention discloses a broadleaf holly leaf buccal tablet capable of reducing high blood pressure, high blood lipid and high blood glucose and a production method of the buccal tablet. The broadleaf holly leaf buccal tablet is characterized being prepared from the following raw materials in parts by weight: 90-100 parts of broadleaf holly leaves, 20-30 parts of momordica grosvenori, 8-12 parts of radix polygonati officinalis, 8-12 parts of rhinacanthus nasutus, 4-8 parts of persimmon leaves, 3-5 parts of radix curcumae longae, 3-5 parts of kelp, 1-2 parts of catharanthus roseus, 1-2 parts of nicotinic acid, 1-2 parts of menthol, 50-70 parts of filler and 10-15 parts of a lubricant. The broadleaf holly leaf buccal tablet capable of reducing high blood pressure, high blood lipid and high blood glucose has the characteristics of reducing high blood pressure, high blood lipid and high blood glucose, clearing away heat and toxic materials, tonifying spleen, calming nerves, losing weight, inhibiting and preventing cancer and the like.

The invention discloses a method for enriching jujube witches' broom phytoplasma by using catharanthus roseus. The method uses catharanthus roseus as a rootstock, and a jujube witches' broom branch asa scion for grafting, then a grafted plant is moved into an artificial climate control room for cultivation, first dark culture is performed for 1 d under conditions of 23 DEG C and 85% relative humidity, and then culture is performed until the onset of illness under conditions of 16 h photoperiod, 23 DEG C and 65% relative humidity. The method relatively well achieves the enrichment of jujube witches' broom phytoplasma, so that problems such as low content of phytoplasma in an original host, separation difficulty and cumbersome operation in the existing jujube witches' broom phytoplasma separation research are relatively well solved, and thereby the foundation for the in-depth research of jujube witches' broom phytoplasma, and how to prevent and treat jujube witches' broom is laid.

The invention discloses a method for quickly extracting the DNA (deoxyribonucleic acid) of huanglongbing pathogen. The method comprises the following steps: sequentially performing freeze-drying and grinding on orange leaves, roots and periwinkle leaves which are infected with huanglongbing, adding a proper amount of NaOH and a proper amount of quartz to separate the DNA of the huanglongbing pathogen through secondary grinding, performing high-speed centrifugation and absolute ethyl alcohol precipitation. The method is simple, safe, quick and low in cost; the extracted DNA solution can be directly used as a template for nest type PCR (polymerase chain reaction) and quantitative PCR detection; the method is suitable for extraction of the DNA of the huanglongbing pathogen in various tissues such as the orange leaves, the roots and the periwinkle leaves which are infected with the huanglongbing; furthermore, according to the extracting method, high-corrosivity reagents such as Tris saturated phenol and chloroform are not needed; the safety of people can be effectively guaranteed.

The invention provides a traditional Chinese medicine for treating liver Qi stagnation type chronic hepatitis B and a preparation method for the traditional Chinese medicine. The traditional Chinese medicine is prepared from the following raw materials: begonia fimbristipula, rhizoma panacis majoris, catharanthus roseus, root of ficus gasparriniana, stem of moupin dutchmanspipe, herb of denseflower bulbophyllum, magnolia officinalis flowers, loquat cores, fiveleaf akebia fruits, hedychium spicatum, mongollian thyme herb, hinn ervtmi root, herb of creeping falsepimpernel, homalocladium platycladum, melastoma dodecandrum roots, pearly maesa, root of kerr treebine, impatiens balsamina, callicarpa longissima and root of Chinese eriosema. The traditional Chinese medicine has the beneficial effects that the traditional Chinese medicine is high in pertinence to the liver Qi stagnation type chronic hepatitis B according to the principle of evidence-based medicine in traditional Chinese medicines, definite in treating effect, quick in response, low in toxic and side effect and low in recurrence rate.

The invention discloses a traditional Chinese medicine for treating deficiency of qi and yin after ovarian cancer chemotherapy. The traditional Chinese medicine is prepared from the following medicinal materials: codonopsis pilosula, astragalus membranaceus, rhizoma atractylodis macrocephalae, radix trichosanthis, schisandra chinensis, radix paeoniae alba, asparagus cochinchinensis, radix ophiopogonis, cortex moutan, radix adenophorae, poria cocos, radix rehmanniae, radix scrophulariae, colla corii asini, angelica sinensis, ligusticum wallichii, rehmannia glutinosa, fructus psoraleae, eclipta alba, fingered citron, rhizoma smilacis glabrae, radix aucklandiae, cornu cervi degelatinatum, arillus longan, solanum lyratum, dysosma versipellis, terfezia leonis, fruit of Chinese wolfberry, sulfur bacteria, spirulina, fried rice sprout, roasted malt, orange peel, cortex lycii radicis, felt covered agaric, caulis spatholobi, catharanthus roseus, cephalotaxus oliveri, fructus amomi, medicated leaven, endothelium corneum gigeriae galli, turtle shell, tortoise plastron, stir-baked squama manitis, raw oyster shell, ground beetle, pinellia ternate, caulis bambusae in taeniam, ginger and liquorice. Clinic practice proves that traditional Chinese medicine is capable of safely and effectively treating the deficiency of qi and yin after ovarian cancer chemotherapy.

This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, this method is characterized in that the gene coding for isochorismate synthase is selected from a group consisting of entC, orfA, pchA and ICS, this last gene preferably the ICS gene from Catharantus roseus. Another embodiment of the invention is a method according to the method described above, characterized in that plants are additionally transformed with an expression cassette harboring a gene coding for an isochorismate pyruvate lyase, preferably on the same expression cassette as the gene coding for isochorismate synthase. The gene coding for isochorismate pyruvate lyase is preferably selected from the group consisting of orfD and pchB. A further aspect of the invention is a protein having isochorismate synthase activity which is isolated from Catharantus roseus. Still a further aspect of the invention is the nucleotide sequence comprising the 5' regulatory region which is naturally found to regulate the expression of the ICS gene in Catharantus roseus.

Compounds, pharmaceutical compositions including the compounds, and methods of preparation and use thereof are disclosed. The compounds are noscapine analogs. The compounds and compositions can be used to treat and / or prevent a wide variety of cancers, including drug resistant cancers. While the antitussive plant alkaloid, noscapine, binds tubulin, displays anticancer activity, and has a safe pharmacological profile in humans, structure-function analyses pointed to a proton at position 9 of the isoquinoline ring that can be modified without compromising tubulin binding activity. Noscapine analogs with various functional moieties at position 9 on the isoquinoline ring kill human cancer cells resistant to other anti-microtubule agents, such as vincas and taxanes. Representative analogs include the 9-nitro, 9-bromo-, 9-iodo-, and 9-fluoro-noscapines, which bind tubulin and induce apoptosis selectively in tumor cells (ovarian and T-cell lymphoma) resistant to paclitaxel, vinblastine and teniposide. Surprisingly, treatment with one of the analogs, 9-nitro-nos, at doses as high as 100 μM, did not affect the cell cycle profile of normal human fibroblasts. This selectivity for cancer cells represents a unique edge over the other available antimitotics. The compounds can perturb the progression of cell cycle by mitotic arrest, followed by apoptotic cell death associated with increased caspase-3 activation and appearance of TUNEL-positive cells. Thus, the compounds are novel therapeutic agents for a variety of cancers, including ovarian and T-cell lymphoma cancers, even those that have become drug-resistant to currently available chemotherapeutic drugs.

The invention discloses a traditional Chinese medicine composition for relieving uneasiness and supplementing qi after operation and a preparation method thereof. The traditional Chinese medicine composition for relieving uneasiness and supplementing qi after operation comprises the following materials: benzoin, lily bulb, cypraeae concha, catharanthus roseus, medulla junci, cecidien, flos albizzia, choerospondias axillaris, Edgeworthia chrysantha Lindl., oenanthe stolonifera, Swertia bifolia Batal, cactus, elm leaves, panax quinquefolium, stigma croci, rhodiola henryi, husked sorghum, yam, alpiniae oxyphyllae fructus, longan aril, rigescent hawksbeard root, lotus seed and wild panax pseudo-ginseng, and further comprises the following auxiliary materials: traditional Chinese medicine nutrient composite powder, bee honey and water soluble vitamin. The preparation method comprises the steps of extracting the effective ingredients of the raw materials to be prepared into extract powder, and preparing the extract powder together with the auxiliary materials into corresponding preparation formulations according to conventional pharmaceutical processes. The traditional Chinese medicine composition disclosed by the invention has a reasonable formula with the materials supplementing each other, has the effects of tranquilizing and allaying excitement, nourishing blood and supplementing qi, strengthening the spleen and replenishing qi, treating consumptive diseases, strengthening the waist and knees, strengthening the liver and stomach, replenishing essence and blood, strengthening tendons and muscles and nourishing heart and easing mind, enhances the immune function of bodies, has a remarkable treatment effect, and is especially suitable for patients of postoperative recovery.