4 results about "Catharanthus roseus" patented technology

The present application belongs to the field of microbial technology, and particularly relates to a fungus in promoting growth of Nauclea officinalis and increasing yield of active substance in a microbial agent. The fungus has a preservation number of GDMCC No: 66543, and belongs to Cladosporium herbarum Cladosporium tenuissimum. Researches show that the fungus can well colonize in the roots of Nauclea officinalis, promote growth of the plant, and increase the yield of vinosamycin lactam in the leaves of Nauclea officinalis, thereby facilitating further improvement of availability of medicinal substance vinosamycin lactam and promoting cultivation of Nauclea officinalis.

The invention discloses an enrichment method suitable for trace leaf plasma membrane protein, and belongs to the technical field of biology. According to the method, a mode of combining column type separation with density gradient centrifugation is adopted, components highly enriched in plasma membrane protein can be obtained through the processes of adjusting the material dosage, the centrifugation mode and the like, and the enrichment purity is improved while the separation time is saved. Compared with an untreated component, the plasma membrane labeled protein H + ATPase in the enriched component is obviously increased; meanwhile, compared with components only subjected to column type separation, chloroplast labeled protein RuBisCO ACT, cell nucleus labeled protein Histone H3 and cytoplasm labeled protein UGP are remarkably reduced. According to the method, an effective system suitable for extracting and purifying the plasma membrane protein of the catharanthus roseus leaves is established for the first time, and the method has reference significance for constructing plasma membrane protein enrichment of other plant leaves.

The invention discloses a method for accurately editing key enzyme genes in a plant secondary metabolic pathway, belongs to the technical field of plant genetic engineering and metabolic engineering, and realizes accurate editing of a plurality of key enzyme genes in the plant secondary metabolic pathway by adopting a CRISPR / Cas9 technology through a multi-target synchronous editing strategy in combination with a tRNA-sgRNA tandem expression system and a homologous recombination repair mechanism. Through the optimized agrobacterium tumefaciens-mediated transformation method, the transformation efficiency is improved to 15-35%. The editing integrity is improved to 55-75%, and the off-target frequency is reduced to 0.3-1.2%. The method is applied to various plants such as sweet wormwood, tomatoes and catharanthus roseus, the content of secondary metabolites such as terpenoids, flavonoids and alkaloids is increased by 3-10 times, and a powerful technical support is provided for efficient production of the secondary metabolites of the plants.

This utility model discloses a periwinkle self-pollinator, comprising: a fixed cylinder with a handle and flower bud clips; a housing fixedly installed on the top surface of the fixed cylinder, the housing having a conveying mechanism for conveying the flower bud clips inside. The flower bud clips are sequentially pushed into the fixed cylinder by the feeding mechanism and then conveyed sequentially to a movable tube by the conveying mechanism. Simultaneously, a moving mechanism causes the movable tube to reciprocate and extend. After the flower bud clips are conveyed into the elastic grippers, the movable tube begins to contract, causing the elastic grippers to close and squeeze the flower bud clips, thus clamping the top of the periwinkle flower buds. When the movable tube extends, the elastic grippers open and separate from the flower bud clips clamping the periwinkle flower buds, thereby achieving the effect of binding the periwinkle flower buds for self-pollination, effectively improving the efficiency of periwinkle self-pollination and better maintaining the purity of self-pollinated seeds.