The invention discloses an integrated
proteomics sample pretreatment platform based on SCX / SAX mixed filler and its application. The
proteomics sample pretreatment platform includes
pipette tip (1), strong cation exchange resin and strong anion Exchange resin mixed packing (2) and
solid-phase extraction membrane (3); Described
solid-phase extraction film (3) is filled in the lower end of
pipette tip (1), strong cation exchange resin and
strong anion exchange resin mixed packing ( 2) Fill in the lower end of the
pipette tip (1) and be located on the
solid phase extraction membrane (3). The
proteomics sample pretreatment platform of the present invention completes the steps of sample pre-enrichment, reduction,
alkylation, and enzymolysis on the mixed
ion exchange packing. The characteristic of the reactor is that the mixed
ion exchange packing used can be used at any pH value
Protein enrichment can be realized under the condition of
enzymatic hydrolysis pH replacement process, less
protein loss,
trypsin can move back and forth between the two fillers, and the
enzymatic hydrolysis efficiency is higher.