45 results about "Protein enrichment" patented technology

The present invention provides methods of selective enrichment of ligands present in a biological sample. One or a plurality of receptor carriers are used to capture ligands capable of binding to receptors immobilized on the surface of the receptor carriers. Receptor carriers bound with the ligands are separated from the remaining sample and the ligands are then eluted with a ligand elution solution to result in an enriched ligand sample. The enriched ligand sample may be used for further isolation of one or more ligands of interest, or for ligand profiling using 2-D gel electrophoresis coupled with mass spectrometry, for example. Such ligand profiling may have a number of applications, such as disease diagnosis, pathogen detection and drug screening.

The invention relates to a method for enriching and purifying glycoproteins. The method combines the hydrazide chemical method for highly selective enrichment of glycoproteins and the use of a centrifugal ultrafiltration device for high-efficiency purification. The hydrazide is completed in situ on the centrifugal ultrafiltration device. Enrichment glycoprotein process. This method carries out the whole glycoprotein enrichment process in situ, which can effectively reduce the sample loss. At the same time, the use of centrifugal ultrafiltration device makes it possible to apply solubilizer in the enrichment process, so as to achieve soluble protein and / or insoluble membrane protein Simultaneous enrichment of glycoproteins; the advantages of effective replacement of solvents by centrifugal ultrafiltration devices enable the introduction of various types of elution solutions during the enrichment of glycoproteins to complete the effective removal of non-glycoproteins, thereby improving the selectivity of glycoprotein enrichment.

A hollow fiber includes a lumen, a polymeric membrane defining the lumen, and a porous tubular substrate, a circumferential surface of which is in contact with a circumferential surface of the polymeric membrane. The polymeric membrane includes a first polymer having monomers each containing an imidazole group. The hollow fiber can be used for water reclamation and protein enrichment

The invention belongs to the technical field of protein enrichment and relates to a method for specific and reversible enrichment of nascent proteins. Label the cells with AHA to extract the whole protein; take the whole protein of the cells and connect the alkyne-based resin through a click reaction; wash the non-specifically adsorbed protein with different buffers to remove the non-specifically adsorbed protein; digest with trypsin to release the target peptide section; samples were collected for mass spectrometry analysis. Compared with the prior art, the present invention achieves highly selective enrichment of newly synthesized low-abundance proteins through biomarkers, selective covalent bonding, stringent elution conditions and selective dissociation, combined with nano‑LC‑ MS / MS enables large-scale analysis and identification of proteins, and nascent proteins can be further identified based on increased mass tags. This method provides an efficient method for the high-efficiency enrichment and accurate identification of nascent proteins, and is widely used in the research field of low-abundance nascent proteomics.

The invention provides an R-CH2-PO3-M modified magnetic nanoparticle as a phosphorylation protein enrichment material, as well as a preparation method and application thereof in phosphorylation protein enrichment. The purpose of combining the functions of magnetic nanoparticles and phosphonic acid groups can be achieved through modifying carboxyl groups for organic phosphonic acid gene R. The synthesis method of the magnetic nano material with functionalized organic phosphonic acid is simple, rapid and green. The material has favorable enrichment effect on phosphorylation proteins, and an MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry) can be used for detecting a phosphorylation peptide segment in 10-8M of bovine serum beta-casein digestive juice. The results show that a type of favorable phosphorylation protein enrichment materials can be constructed through combining the magnetic nanoparticles with the organic phosphonic acid.

The invention belongs to the technical field of biotechnology and specifically relates to a hydrophilic branch and C18 long-chain modified magnetic graphene material as well as a preparation method and application thereof in protein enrichment. The method comprises the following steps: utilizing a hydrothermal method to compound a high-stability magnetic graphene material; coating a layer of silicon dioxide on the surface according to a reversed-phase micro-emulsion method; adding amino and C18 silylation reagent; and connecting PAMAM with amino according to a glutaraldehyde crosslinking method, thereby acquiring the hydrophilic-hydrophobic dendritic molecule and C18 long-chain modified magnetic graphene material. The modified PAMAM molecule has higher hydrophilicity, so that the dispersibility of the material in water is better; the C18 long chain has higher hydrophobicity and is easy to enrich protein; in the enrichment for four standard proteins, the recovery rate is as high as above 80%; in the enrichment for standard protein BSA and myohemoglobin MYO, the limit of detection is as low as 1 ng / muL; the hydrophilic branch and C18 modified magnetic graphene material is practical and efficient, is high in repeatability, is high in stability and is wide in application prospect.

The invention discloses an integrated proteomics sample pretreatment platform based on SCX / SAX mixed filler and its application. The proteomics sample pretreatment platform includes pipette tip (1), strong cation exchange resin and strong anion Exchange resin mixed packing (2) and solid-phase extraction membrane (3); Described solid-phase extraction film (3) is filled in the lower end of pipette tip (1), strong cation exchange resin and strong anion exchange resin mixed packing ( 2) Fill in the lower end of the pipette tip (1) and be located on the solid phase extraction membrane (3). The proteomics sample pretreatment platform of the present invention completes the steps of sample pre-enrichment, reduction, alkylation, and enzymolysis on the mixed ion exchange packing. The characteristic of the reactor is that the mixed ion exchange packing used can be used at any pH value Protein enrichment can be realized under the condition of enzymatic hydrolysis pH replacement process, less protein loss, trypsin can move back and forth between the two fillers, and the enzymatic hydrolysis efficiency is higher.