Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
45 results about "Protein enrichment" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Basically, protein enrichment is a technique where proteins of interest in a biological sample are concentrated to make them more suitable for identification and downstream analysis. In most cases,...
Multifunctional double-layer core-shell structure magnetic nano particle, preparation method and application thereof
InactiveCN101923932ARapid enrichmentRapid separation and purificationInorganic non-active ingredientsInorganic material magnetismMagnetite NanoparticlesSuperparamagnetism
The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.
Owner:NANJING UNIV
Dual-layer hollow fibers with enhanced flux as forward osmosis membranes for water reuses and protein enrichment
A hollow fiber includes a lumen, a polymeric membrane defining the lumen, and a porous tubular substrate, a circumferential surface of which is in contact with a circumferential surface of the polymeric membrane. The polymeric membrane includes a first polymer having monomers each containing an imidazole group. The hollow fiber can be used for water reclamation and protein enrichment
Owner:NAT UNIV OF SINGAPORE
Trace polypeptide or protein-enriched and its direct analyzing method
InactiveCN1560625AGuaranteed reproducibilityGuaranteed reliabilityComponent separationParticle adsorptionDirect analysis
The invention is a method for carrying on enrichment-groundmass assistant laser analyzing ionized mass spectrum direct analysis to trace peptide and protein product using zeolite nanometer particles as nanometer micro absorbing agent. The zeolite nanometer particle has excellent absorbing effect to the protein and the polypeptide, and it has good compatibility to the groundmass assistant laser analyzing ionized spectrum, the sample absorbed by the zeolite nanometer particle needs no sample rinsing step to carry on groundmass assistant laser analyzing ionized spectrum analysis directly, the operation is simple, avoids the sample loss in rinsing process. The enrichment efficiency to polypeptide in the invention can reach to 1250 times, the protein enzyme sample with 50X10 to the power -12g / micro litre can be testified by the invention.
Owner:FUDAN UNIV
Method of selective protein enrichment and associated applications
Owner:LEAP BIOSCI CORP
Integrated proteomic sample pretreatment platform based on SCX (strong cation exchange)/SAX (strong anion exchange) mixed filling material and application thereof
ActiveCN107402269AEfficient enzymatic hydrolysisEnable mass authenticationComponent separationAlkyl transferPipette
The invention discloses an integrated proteomic sample pretreatment platform based on an SCX (strong cation exchange) / SAX (strong anion exchange) mixed filling material and application thereof. The proteomic sample pretreatment platform comprises a pipette gun head (1), strong cation exchange resin and strong anion exchange resin mixed filling materials (2) and a solid phase extraction film (3), wherein the solid phase extraction film (3) is loaded in the lower end of the pipette gun head (1); the strong cation exchange resin and strong anion exchange resin mixed filling materials (2) are loaded in the lower end of the pipette gun head (1), and are positioned above the solid phase extraction film (3). The proteomic sample pretreatment platform has the advantages that the steps of sample preenrichment, reduction, alkylation and enzymolysis are completed on the mixed ion exchange filling materials; a reactor is characterized in that the used mixed ion exchange filling materials can realize the protein enrichment at any pH values; in the enzymolysis pH replacement process, the protein loss is little; trypsinase can move back and forth between the two kinds of filling materials; the enzymolysis efficiency is higher.
Owner:深圳深维超生物科技有限公司
Preparation of organic phosphonic acid functionalized magnetic nano material and application in protein enrichment
InactiveCN101923934ARealize the combinationImprove bindingComponent separationOrganic/organic-metallic materials magnetismSynthesis methodsPhosphorylation
The invention provides an R-CH2-PO3-M modified magnetic nanoparticle as a phosphorylation protein enrichment material, as well as a preparation method and application thereof in phosphorylation protein enrichment. The purpose of combining the functions of magnetic nanoparticles and phosphonic acid groups can be achieved through modifying carboxyl groups for organic phosphonic acid gene R. The synthesis method of the magnetic nano material with functionalized organic phosphonic acid is simple, rapid and green. The material has favorable enrichment effect on phosphorylation proteins, and an MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry) can be used for detecting a phosphorylation peptide segment in 10-8M of bovine serum beta-casein digestive juice. The results show that a type of favorable phosphorylation protein enrichment materials can be constructed through combining the magnetic nanoparticles with the organic phosphonic acid.
Owner:NANJING UNIV
Sugar protein enrichment purification method
ActiveCN104710506AReduce lossesAchieve the purpose of simultaneous enrichmentPeptide preparation methodsPurification methodsUltrafiltration
The invention relates to a sugar protein enrichment purification method, according to the method, hydrazide chemical method for high selective sugar protein enrichment and a centrifugal ultrafiltration device for efficient purification are integrated, hydrazide sugar protein enrichment process can be completed in the centrifugal ultrafiltration device, the whole sugar protein enrichment process can be completed in situ, the sample loss can be effectively reduced, at the same time, by use of the centrifugal ultrafiltration device, a solubilizing agent can be applied in the enrichment process so as to realize sugar protein simultaneous enrichment in soluble protein and / or insoluble membrane protein; by use of the advantages of effective solvent replacement of the centrifugal ultrafiltration device, various types of elution solutions can be introduced into the sugar protein enrichment process to complete effective removal of non sugar protein so as to enhance the selectivity of sugar protein enrichment.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Protein enrichment detection device based on light-operated fluid transportation and magnetic control sample separation
ActiveCN111774106AReduce volumeHigh control precisionLaboratory glasswaresFluorescence/phosphorescenceProtein detectionProtein target
The invention relates to a protein enrichment detection device based on light-operated fluid transportation and magnetic control sample separation. The interior of an optical flow control micro-tube actuator is used for accommodating magnetic nanoparticles, a to-be-detected liquid sample and a detection liquid and is a place for capturing the target protein of magnetic nano particles and reactionbetween the target protein and detection liquid. An electromagnetic control device is arranged in the middle of the exterior of the optical flow control micro-tube actuator, and exertion and cancellation of a magnetic field are achieved by controlling on-off of a power supply; a control light source is arranged above the outer part of the optical flow control micro-tube actuator and is used for driving liquid in the optical flow control micro-tube actuator to finish directional movement; the detection tube is connected with one end of the optical flow control micro-tube actuator, and the optical detection instrument is used for optical detection of liquid in the detection tube. Compared with the prior art, non-contact control can be achieved, portable protein detection can be truly achieved, and meanwhile the risk that a sample is polluted is effectively avoided.
Owner:FUDAN UNIV
Glycopeptide or glycoprotein enrichment material, preparation thereof and application of material
InactiveCN109482150AEasy to makeImprove reaction efficiencyOther chemical processesPeptide preparation methodsChemical reactionDisulfide bond
The invention belongs to the technical field of glycopeptide / glycoprotein enrichment materials and analysis, and relates to a sugar chain releasable glycosylated peptide fragment / protein enrichment material based on a hydrazide strategy, a preparation method of the material and an application of the material. The surface of a matrix micro-sphere is bonded with a hydrazide compound containing disulfide bonds to form a functional material with a hydrazide group on the surface, wherein the functional material can be used for glycopeptide enrichment. The disulfide bonds are led to the surface of the material to realize sugar chain releasable enrichment of glycopeptide / glycoprotein, sugar chains are in covalent binding, and the material has the advantage of high enrichment efficiency and overcomes the shortcoming that the sugar chains are not easily released in a traditional hydrazide method and cannot be used for O-glycosylated peptide fragment / protein enrichment. The sugar chain releasable enrichment material is easy to prepare and good in enrichment selectivity, is widely applicable to N-glycosylated and O-glycosylated peptide fragment / protein enrichment and has a high practical value in the fields of glycoproteomics and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Flavone glycoside functionalized magnetic nanometer affinity probe, preparation method and applications thereof, and intracellular target protein capture method
ActiveCN108693354AHigh capture specificityAchieve captureMaterial nanotechnologyNanomedicineProtein targetCoupling
The invention relates to the field of drug target protein enrichment, separation and detection, and discloses a flavone glycoside functionalized magnetic nanometer affinity probe, and further providesa preparation of the flavone glycoside functionalized magnetic nanometer affinity probe, applications of the flavone glycoside functionalized magnetic nanometer affinity probe in the drug target protein capture and / or identification of cells, and a method for capturing an intracellular target protein through the flavone glycoside functionalized magnetic nanometer affinity probe, wherein the flavone glycoside functionalized magnetic nanometer affinity probe comprises magnetic nanoparticles, a coupling structure and a flavone glycoside compound supported on the surface of the magnetic nanoparticle, one end of the coupling structure is coupled to the surface of the magnetic nanoparticles, and the other end of the coupling structure is coupled to the flavone glycoside compound. With the flavone glycoside functionalized magnetic nanometer affinity probe of the present invention, the high-specificity capture can be achieved only with a small amount of the whole protein extraction liquid.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Specific reversible newborn protein enrichment method
ActiveCN111777696AStable structureHigh enrichment capacityPreparing sample for investigationEnzyme digestionTarget peptide
The invention belongs to the technical field of protein enrichment, and relates to a specific reversible newborn protein enrichment method which comprises steps: marking cells with AHA, and extractingtotal proteins; taking cell total proteins and an alkynyl resin to be connected through click reaction; washing non-specifically adsorbed proteins with different buffer solutions to remove the non-specifically adsorbed proteins; carrying out enzyme digestion by trypsin to release a target peptide fragment; collecting samples for mass spectrometry. Compared with the prior art, according to the method, high-selectivity enrichment of a newly synthesized low-abundance protein is achieved through biolabeling, selective covalent bonding, strict elution conditions and selective dissociation, large-scale analysis and identification of protein can be achieved by combining nano-LC-MS / MS, and the newly-generated protein can be further identified according to the added mass label. The method providesan efficient method for efficient enrichment and accurate identification of the newborn protein, and is widely applied to the research field of low-abundance new proteomics.
Owner:FUDAN UNIV
N-phosphopeptide and protein enrichment material and preparation and application thereof
ActiveCN108079957AMass transfer speedRapid enrichmentOther chemical processesPreparing sample for investigationTert-Butyloxycarbonyl protecting groupMicrosphere
The present invention relates to a novel silica gel material capable of recognizing N-phosphopeptides and proteins under neutral conditions and application thereof in N-phosphopeptide and protein enrichment. Non-porous silica microspheres are prepared by a seed growth method, a core-shell microsphere initial layer is formed by a template-guided dissolution and re-deposition method, and finally sub-2-micron core-shell silica gel with vertical pores can be obtained by acid reflux. N-tert-butyloxycarbonyl-L-tyrosine and N, N'-dipicolylamine are reacted to form a support molecule, and a phosphaterecognition functional molecule is formed by complexation of a metal ion on the support molecule. Finally, the novel phosphoric acid recognition functional silica gel material is obtained by covalently bonding the phosphoric acid recognition functional molecule with the sub-2-micron core-shell silica gel having the vertical pores through an amide bond. The novel phosphoric acid recognition functional silica gel material can rapidly and specifically enrich the N-phosphopeptides and proteins in a neutral buffer system.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
A kind of glycoprotein enrichment and purification method
ActiveCN104710506BReduce lossesAchieve the purpose of simultaneous enrichmentPeptide preparation methodsUltrafiltrationCell Membrane Proteins
The invention relates to a method for enriching and purifying glycoproteins. The method combines the hydrazide chemical method for highly selective enrichment of glycoproteins and the use of a centrifugal ultrafiltration device for high-efficiency purification. The hydrazide is completed in situ on the centrifugal ultrafiltration device. Enrichment glycoprotein process. This method carries out the whole glycoprotein enrichment process in situ, which can effectively reduce the sample loss. At the same time, the use of centrifugal ultrafiltration device makes it possible to apply solubilizer in the enrichment process, so as to achieve soluble protein and / or insoluble membrane protein Simultaneous enrichment of glycoproteins; the advantages of effective replacement of solvents by centrifugal ultrafiltration devices enable the introduction of various types of elution solutions during the enrichment of glycoproteins to complete the effective removal of non-glycoproteins, thereby improving the selectivity of glycoprotein enrichment.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Concentrated milk with improved taste
Proposed is concentrated milk with the improved taste, and the concentrated milk can be obtained by: (a) separating raw milk or whole milk in a method known per se to obtain a skimmed milk fraction and a cream fraction; (b) subjecting the skimmed milk fraction obtained from step (a) to nanofiltration and obtaining a mineral-enriched permeate P1 and a partially mineral-removed and protein-enrichedretentate R1; (c) mixing the retentate R1 with at least part of the cream fraction obtained from step (a); (d) optionally dehydrating the mixture obtained from step (c) to obtain a concentrate; (e) mixing the concentrate obtained in step (d) or the mixture obtained in step (c) with a buffer containing or consisting of at least one phosphate and at least one hydroxycarboxylate to obtain a mixture;(f) homogenizing the mixture obtained in the step (e) to obtain a homogenized product; and (g) pasteurizing the homogenate obtained from step (f).
Owner:DMK DEUT MILCHKONTOR
Pretreatment method, preservation method, automatic treatment system and detection method of urine sample
PendingCN114354333AStable quantityQuality improvementComponent separationPreparing sample for investigationPolyvinylidene difluoridePolyvinylidene fluoride
The invention discloses a pretreatment method, a preservation method, an automatic treatment system and a detection method of a urine sample, and relates to the technical field of biological detection.The pretreatment method comprises the steps that the urine sample subjected to protein splitting is subjected to reductive alkylation treatment, and then protein enrichment and enzymolysis are conducted; in the protein enrichment step, a PVDF (Polyvinylidene Fluoride) filter plate is adopted to carry out protein enrichment on the sample subjected to reductive alkylation treatment. The invention further provides an automatic urine sample treatment system and an automatic sample treatment method. The treatment system greatly reduces the labor intensity of people, facilitates the improvement of the treatment efficiency of urine sample treatment, meets the high-flux and automatic proteomics pretreatment requirements, and adapts to the reproducibility and flux of the current clinical requirements.
Owner:JIANGSU QLIFE MEDICAL TECH GRP CO LTD
Protein enriching device
InactiveCN107903299AThe effect of enrichment solution is goodLarge amount of processingPeptide preparation methodsEngineeringFoam separation
The invention discloses a protein enriching device. The protein enriching device mainly comprises a shell, a water drawing pump and a foam separation device, wherein a stirring device, a centrifugingdevice and a ball milling device are arranged in the shell and are used for extracting protein; the foam separation device is used for enriching the protein. Through the protein enriching device, liquid supernatant can be obtained by only adjusting a control device and a driving device to crush and centrifuge cells in raw liquid; the process is simple; the manpower and the time are saved; meanwhile, protein enriching liquid obtained by a foam separation method is excellent in effect, simple in treatment process, high in treatment capacity and low in cost and low in energy consumption.
Owner:湖北普罗金科技有限公司
Photo-induced multifunctional cross-linking agent, preparation method and application thereof
PendingCN112574089AExtended half-lifeGood cross-linking efficiencyUrea derivatives preparationCarbamic acid derivatives preparationLysisMass Spectrometry-Mass Spectrometry
The invention relates to a photo-induced multifunctional cross-linking agent as shown in a general formula (I), wherein the photo-induced multifunctional cross-linking agent is mainly applied to biomacromolecule interaction, such as protein-protein interaction and protein-nucleic acid interaction. According to the invention, the cross-linking agent can be used in biological samples or cells of cell lysis solutions to capture protein-protein interaction or protein-nucleic acid interaction, and can be applied to subsequent protein enrichment and protein cross-linking mass spectrometry; and the photo-induced multifunctional cross-linking agent has important application potential and practical value in interaction research of biomacromolecules.
Owner:SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Method for efficiently enriching cell protein of buffalo
ActiveCN108191953AReduce usageImprove extraction efficiencyPeptide preparation methodsNon-contact ultrasoundMagnetic bead
The invention relates to the technical field of protein extraction, in particular to a method for efficiently enriching cell protein of a buffalo. The method comprises the steps of cell pyrolysis, preparation of a protein sample, purification of protein, recycling of peptide fragments and the like, the method of combining non-contact ultrasonic sample crushing and protein enrichment by magnetic beads is adopted, the sample dosage is greatly lowered, and the extracting efficiency of protein is improved. The method has high operating practicability and is easy to popularize, an effective sampletreatment method is provided for a proteomic technology to study micro-samples or precious samples so that the method can satisfy the sample condition needed by the proteomic study. By means of the sample treatment method, the bottleneck problem that currently, the micro-samples cannot satisfy the sample dosage needed by the proteomic study is solved, a feasible scheme is also provided for the unicellular proteomic analysis, and the cell protein of the buffalo is efficiently enriched.
Owner:GUANGXI UNIV
Puffed black fungus powder and preparation method thereof
The invention discloses puffed black fungus powder and a preparation method thereof. The preparation method comprises the following steps of cleaning black fungi, and mashing into small blocks; drying the black fungi at the drying temperature of 40 to 60DEG C for 30 to 50 minutes, and enabling the water content to be 8 to 30 percent; performing vacuum microwave puffing treatment on the black fungi, wherein the microwave power is 650 to 780W, the puffing time is 5 to 12 minutes, and the vacuum degree is 0.05 to 0.10MPa; feeding the puffed black fungi into a crusher for crushing to 100 meshes, thus obtaining the black fungus powder. The puffing rate of the black fungi is 250 to 450 percent. According to the puffed black fungus powder and the preparation method thereof disclosed by the invention, firstly, the black fungi are puffed and are then crushed, so that microstructures of the black fungi are changed, the enrichment ratio of protein as well as the dissolution rate, the solubility, the medicinal effect and the bioavailability of functional substances such as polysaccharide and the like are remarkably improved, and quick absorption and intake of multiple nutrients by a human body are facilitated.
Owner:HEILONGJIANG ACADEMY OF SCI
Dual-layer hollow fibers with enhanced flux as forward osmosis membranes for water reuses and protein enrichment
A hollow fiber includes a lumen, a polymeric membrane defining the lumen, and a porous tubular substrate, a circumferential surface of which is in contact with a circumferential surface of the polymeric membrane. The polymeric membrane includes a first polymer having monomers each containing an imidazole group. The hollow fiber can be used for water reclamation and protein enrichment
Owner:NAT UNIV OF SINGAPORE
High-specificity cell secreted protein enrichment method
PendingCN114075588AMaterial analysis by electric/magnetic meansPeptide preparation methodsBiotechnologyDisease
Research of secretory proteomics has wide application in the aspects of early diagnosis of diseases and treatment of diseases. A traditional secretory protein enrichment method mostly adopts serum-free culture, but the culture mode has various defects. The invention provides a protein enrichment process for two-step peptide fragment layer enrichment, and the specificity of the method is greatly improved. Meanwhile, according to the invention, an unnatural amino acid, namely Azidohomoalanine (AHA), which can be used for replacing methionine is introduced to mark the protein synthesized by the cells per se. And in combination with a click chemical reaction which is widely applied in recent years, alkynyl modified biotin (biotin) can be efficiently added to an insertion site of AHA. With the help of biotin as a marker, separation, purification and identification of secreted protein in a serum-containing culture medium can be realized.
Owner:SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
Method for enriching sulfydryl nitroso protein in serum
The invention belongs to the technical field of medicines, and particularly relates to a method for enriching sulfydryl nitroso protein in serum. According to the method, firstly, high-abundance interference proteins (albumin and immune globulin) in a serum sample are selectively removed, and then nitrosolated proteins in serum are further enriched through a specific labeling and affinity chromatography combined method. The method disclosed by the invention is good in specificity and simple to operate, and the problems of complex operation, large impurity influence and low enrichment efficiency of the existing mercaptonitrosylated protein enrichment are solved.
Owner:FUZHOU UNIV
A method for specific and reversible enrichment of nascent proteins
ActiveCN111777696BImprove digestion efficiencyImprove reaction efficiencyPreparing sample for investigationTarget peptideAlkyne
The invention belongs to the technical field of protein enrichment and relates to a method for specific and reversible enrichment of nascent proteins. Label the cells with AHA to extract the whole protein; take the whole protein of the cells and connect the alkyne-based resin through a click reaction; wash the non-specifically adsorbed protein with different buffers to remove the non-specifically adsorbed protein; digest with trypsin to release the target peptide section; samples were collected for mass spectrometry analysis. Compared with the prior art, the present invention achieves highly selective enrichment of newly synthesized low-abundance proteins through biomarkers, selective covalent bonding, stringent elution conditions and selective dissociation, combined with nano‑LC‑ MS / MS enables large-scale analysis and identification of proteins, and nascent proteins can be further identified based on increased mass tags. This method provides an efficient method for the high-efficiency enrichment and accurate identification of nascent proteins, and is widely used in the research field of low-abundance nascent proteomics.
Owner:FUDAN UNIV
Preparation of organic phosphonic acid functionalized magnetic nano material and application in protein enrichment
InactiveCN101923934BRealize the combinationImprove bindingComponent separationOrganic/organic-metallic materials magnetismSynthesis methodsPhosphorylation
The invention provides an R-CH2-PO3-M modified magnetic nanoparticle as a phosphorylation protein enrichment material, as well as a preparation method and application thereof in phosphorylation protein enrichment. The purpose of combining the functions of magnetic nanoparticles and phosphonic acid groups can be achieved through modifying carboxyl groups for organic phosphonic acid gene R. The synthesis method of the magnetic nano material with functionalized organic phosphonic acid is simple, rapid and green. The material has favorable enrichment effect on phosphorylation proteins, and an MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry) can be used for detecting a phosphorylation peptide segment in 10-8M of bovine serum beta-casein digestive juice. The results show that a type of favorable phosphorylation protein enrichment materials can be constructed through combining the magnetic nanoparticles with the organic phosphonic acid.
Owner:NANJING UNIV
Construction and application of membraneless organelles in prokaryotes
ActiveCN112575016APromote exchangeRetain biological activityBacteriaAntibody mimetics/scaffoldsEscherichia coliChemical synthesis
The invention discloses construction and application of a membraneless organelle in prokaryotic escherichia coli. According to the method, an expression vector of recombinant spider silk protein or arthropod-like elastin of protein consisting of a tandem mass of repeated peptide segment monomers of nephila cavaleriei dragline silk protein MaSp1 shown as Seq ID No. 1 and protein consisting of a tandem mass of MaSp2 repeated peptide segment monomers shown as Seq ID No. 2 is constrcuted, and is introduced into an expression host; after induced expression, a soluble protein enrichment phase, namely a membrane-free chamber, is formed; and component protein in the membrane-free area exists in a soluble state, and the biological activity of the component protein is reserved to the maximum extent.According to the method, 1,3-propane diamine is produced through the membraneless organelle in escherichia coli for the first time, and the nanoparticles are synthesized in the membraneless organellefor the first time, so that the method is milder and more environment-friendly than a chemical synthesis method.
Owner:SHANGHAI JIAO TONG UNIV
Stable concentrated milk
Proposed is a concentrated milk with the improved stability, and the concentrated milk can be obtained by: (a) separating raw milk or whole milk by means of a method known per se to obtain a skimmed milk fraction and a cream fraction; (b) subjecting the skimmed milk fraction obtained from step (a) to nanofiltration and obtaining a mineral-enriched permeate P1 and a partially mineral-removed and protein-enriched retentate R1; (c) mixing the retentate R1 with at least part of the cream fraction obtained from step (a); (d) optionally dehydrating the mixture obtained from step (c) to obtain a concentrate; (e) mixing the concentrate obtained from step (d) or the mixture obtained from step (c) with at least one hydrocolloid and at least one buffer to obtain a mixture; (f) homogenizing the mixture obtained in the step (e) to obtain a homogenized product; and (g) pasteurizing the homogenate obtained from step (f).
Owner:DMK DEUT MILCHKONTOR
A kind of hydrophilic branch and c18 modified magnetic graphene material and its preparation method and application
ActiveCN106732464BHigh recovery rateImprove efficiencyOther chemical processesPeptide preparation methodsCompound aSilylation
Owner:FUDAN UNIV
Integrated proteomics sample pretreatment platform and its application based on scx/sax mixed filler
ActiveCN107402269BEfficient enzymatic hydrolysisEnable mass authenticationComponent separationEnzymatic hydrolysisIon exchange
The invention discloses an integrated proteomics sample pretreatment platform based on SCX / SAX mixed filler and its application. The proteomics sample pretreatment platform includes pipette tip (1), strong cation exchange resin and strong anion Exchange resin mixed packing (2) and solid-phase extraction membrane (3); Described solid-phase extraction film (3) is filled in the lower end of pipette tip (1), strong cation exchange resin and strong anion exchange resin mixed packing ( 2) Fill in the lower end of the pipette tip (1) and be located on the solid phase extraction membrane (3). The proteomics sample pretreatment platform of the present invention completes the steps of sample pre-enrichment, reduction, alkylation, and enzymolysis on the mixed ion exchange packing. The characteristic of the reactor is that the mixed ion exchange packing used can be used at any pH value Protein enrichment can be realized under the condition of enzymatic hydrolysis pH replacement process, less protein loss, trypsin can move back and forth between the two fillers, and the enzymatic hydrolysis efficiency is higher.
Owner:深圳深维超生物科技有限公司
Method for enriching phosphorylation peptide fragments and proteins and application
PendingCN109897087AMore responsiveAchieve specific identificationPeptide preparation methodsBiological testingTert-Butyloxycarbonyl protecting groupTyrosine
The invention relates to functional molecules for specifically recognizing phosphorylation peptide fragments and proteins in a neutral buffered solution and application. Through a reaction of N-t-butyloxycarboryl-L-tyrosine and N, N'-dipicolylamine, supporting molecules are formed, and metal ions are complexed onto the supporting molecules to form phosphoric acid recognition functional molecules;the phosphoric acid recognition functional molecules are covalently bound with carboxylated adamantane ammonium, and guest molecules with enriched groups are obtained; cucurbituril is covalently modified on the surfaces of silicon spheres, and main molecular functional materials are obtained; and finally, the phosphorylation peptide fragments and the proteins are enriched by capturing the guest molecules in the solution through main materials. The novel phosphoric acid recognition functional molecules can realize liquid phase recognition and solid phase separation of N-phosphorylated peptide fragments and the proteins under a neutral buffer system.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com