34results about How to "Efficient enzymatic hydrolysis" patented technology

The invention relates to a method for separating corncob cellulose by using ionic liquid. According to the invention, an ionic liquid 1-allyl-3-methylimidazole chlorine salt is synthesized with raw materials of allyl chloride and 1-methylimidazole; and with the high solubility of the ionic liquid upon cellulose, the ionic liquid is used for separating and extracting cellulose in corncob. The method comprises the steps of corncob pretreatment, lignin removing, cellulose separation, cellulose extraction, and the like. The extraction rate of corncob cellulose is approximately 18.35%. With the method, the purity of the extracted corncob is high, and the film-forming property of the cellulose is good. According to the invention, a green solvent which is the ionic liquid is used as a dissolving medium. The solvent can be recovered and reused, such that the requirement of green production is satisfied.

The invention discloses a method for preparing butyl alcohol through fermenting by utilizing a lignocellulose raw material. The method comprises the following steps: (1) pre-treating the lignocellulose raw material; (2) adopting Trichoderma viride F4 for performing enzymatic hydrolysis on the pre-treated raw material, thereby acquiring a hydrolyzed mixed liquid glucose of cellulose and hemicellulose, wherein the Trichoderma viride has been preserved at China General Microbiological Culture Collection Center on November 10, 2008 and the preservation No. is CGMCC No. 2736; (3) utilizing calcium hydroxide to perform detoxification treatment on the hydrolyzed mixed liquid glucose; (4) taking the detoxified liquid glucose as a carbon source, replenishing nutrient elements and preparing a fermenting culture medium; and (5) inoculating butylic zymophyte into the fermenting culture medium, fermenting and preparing butyl alcohol. According to the method disclosed by the invention, the cellulose and the hemicellulose can be simultaneously subjected to enzymatic hydrolysis, the hydrolysate is utilized as a fermentation substrate for preparing butyl alcohol and the method has the characteristics of good enzymatic hydrolysis effect, high fermentation yield, and the like.

The invention belongs to the technical field of biomass application, and discloses a method for improving lignin fractional separation and depolymerization of biomass and an application thereof. The method comprises the following steps: cooking treated biomass with a pretreatment solution at 120-173 DEG C, and taking acid as a catalyst, wherein the pretreatment solution comprises an organic solvent and water; wherein the organic solvent is C2-C5 and an organic solvent containing more than two hydroxyl groups, and obtaining biomass containing cellulose and lignin and a pretreatment solution containing lignin; separating and washing the pretreatment solution, combining the pretreatment solution containing lignin, mixing the combined pretreatment solution with water, and carrying out precipitation, filtration and drying treatment to obtain lignin; mixing lignin, an organic solvent and a depolymerization catalyst, and carrying out catalytic depolymerization reaction at 180-250 DEG C in a protective atmosphere to obtain the lignin monomer. According to the method, the depolymerizability of the lignin and the yield of the monophenol compound are improved, and the lignin monomer has morebeta-O-4 bonds and less condensed phenolic groups.

The invention discloses a method for producing butyl alcohol through fermenting by utilizing a lignocellulose raw material. The method comprises the following steps: (1) pre-treating the lignocellulose raw material; (2) adopting Aspergillus niger T2 for performing enzymatic hydrolysis on the pre-treated raw material, thereby acquiring a hydrolyzed mixed liquid glucose of cellulose and hemicellulose, wherein the Aspergillus niger is the Aspergillus niger T2 said in patent CN 200910011768.8; (3) utilizing calcium hydroxide to perform detoxification treatment on the hydrolyzed mixed liquid glucose; (4) taking the detoxified liquid glucose as a carbon source, replenishing nutrient elements and preparing a fermenting culture medium; and (5) inoculating Clostridium beijerinckii XH0906 into the fermenting culture medium, fermenting and preparing butyl alcohol. The Clostridium beijerinckii XH0906 has been preserved at China General Microbiological Culture Collection Center on May 04, 2014 and the preservation No. is CGMCC No.9124. According to the method disclosed by the invention, the Aspergillus niger is utilized to perform enzymatic hydrolysis on the cellulose and the hemicelluloses, the xylose and glucose in the hydrolysate are utilized to ferment and produce butyl alcohol and the method has the characteristics of good enzymatic hydrolysis effect, high fermentation yield, and the like.

The invention discloses a method for carrying out enzymatic hydrolysis. The method comprises the following steps: adding water into protein, evenly stirring, transferring the mixture to an electrophoresis tank, carrying out electrophoresis under a voltage of 80 to 120 V, collecting the protein solution after electrophoresis, dissolving the protein by an enzymatic hydrolysis buffer solution containing trifluoroethanol, subjecting the protein to reduction and alkylation treatments, adjusting the pH value of processed protein to 7.5-8.5, then adding trypsase accounting for 90 to 98% of the protein solution, and carrying out enzymatic hydrolysis to obtain polypeptide; wherein the work electrode in the electrophoresis tank is modified by oxidation-reduction enzyme and Zn-Al layered double hydroxides. The provided method can obtain peptides suitable for liquid chromatogram-mass spectrum (LS-MS) analysis, and provides stable and reliable samples for proteomics analysis.

The invention relates to a method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology, belonging to the field of analyticalchemistry. The method comprises the following steps: selecting the medicinal powder of Scutellaria baicalensis, and carrying out infrared-assisted enzymatic hydrolysis at first, i.e., adding water ora buffer solution with a certain pH value as an enzymatic hydrolysis solution, subjecting the medicinal powder of Scutellaria baicalensis to infrared radiation with a certain power at a certain temperature for a certain period of time, and then adding an organic solvent for infrared-assisted extraction; and then subjecting the obtained extract to filtering at first and then to detection and analysis with a high-peformance liquid chromatograph. The infrared-assisted enzymatic hydrolysis technology provided by the invention is sufficient in enzymatic hydrolysis, fast in speed, high in efficiency, safe, friendly to environment, and beneficial for extraction of the total flavonoid aglycone of Scutellaria baicalensis in the medicinal material Scutellaria baicalensis; and the method is of greatsignificance to development and utilization of the medicinal material Scutellaria baicalensis and to preparation of the total flavonoid aglycone of Scutellaria baicalensis.

The invention discloses a corn stalk pre-processing method, which is used for pre-processing corn stalks by virtue of ethylene glycol assisted NMMO (N-methyl-N-morpholine oxide), wherein the method comprises the following steps: 1, pre-processing a corn stalk raw material by virtue of an NMMO-ethylene glycol-water mixed system, so that a regenerated cellulose material is obtained, wherein the mass of the NMMO is 70-80% of the NMMO-ethylene glycol-water mixed system and the mass of ethylene glycol is 5-15% of the NMMO-ethylene glycol-water mixed system; and 2, washing the generated cellulose material by virtue of de-ionized water, adding an acetic acid-sodium acetate buffer solution, so that a mixed system is formed, and then conducting enzymatic hydrolysis on a constant-temperature shaker with the addition of cellulase. The corn stalk pre-processing method provided by the invention can achieve efficient enzymatic hydrolysis of the corn stalks and reduce an NMMO dosage, so that processing cost is reduced.

The invention discloses a white gourd high-fiber beverage. The white gourd high-fiber beverage comprises the following raw materials of honey, sodium carboxymethyl cellulose, arabic gum, white gourd peel, white gourd juice and a grape extracting solution, wherein the white gourd high-fiber beverage comprises the following preparation steps of (1) drying and crushing the white gourd peel; (2) performing freezing treatment: uniformly mixing the white gourd peel powder with the white gourd juice in proportion, and performing freezing for 24-30h to obtain cooling liquid; (3) performing enzymolysis; and (4) performing filtering and performing bottling: filtering 200 parts by weight of the enzymatic hydrolysate with a plate-frame filter, taking filtrate, adsorbing pigment with an absorption column using white gourd peel carbon as a filler, collecting separated liquid, adding 20-30 parts by mass of the honey, 2 parts by mass of the sodium carboxymethyl cellulose and 3-5 parts by mass of the arabic gum, performing stirring for 10min, and then performing pasteurization and performing bottling, so as to obtain the white gourd high-fiber beverage. The prepared white gourd high-fiber beverageis rich in dietary fibers, water-soluble sugar and multiplex vitamins, and has broad market promotion value.

The invention discloses a method for preparing chitosan oligosaccharide, chitosanase as well as a gene, an enzyme preparation and application of the chitosanase, and belongs to the technical field of hydrolase. The method for preparing the chitosan oligosaccharide comprises the following step: carrying out enzymolysis on chitosan or colloid chitin by adopting chitosanase as shown in SEQ ID NO.1 under the conditions that the pH is 4.0-8.0 and the temperature is 50-80 DEG C to produce the chitosan oligosaccharide. The amino acid sequence of the chitosanase disclosed by the invention is as shown in SEQ ID NO. 1, and the gene for coding the chitosanase is as shown in SEQ ID NO. 2. The enzyme preparation provided by the invention contains the chitosanase. The application of the chitosanase and the enzyme preparation in degradation of chitosan or colloid chitin and the application in preparation of chitosan oligosaccharide are provided. The chitosan oligosaccharide is prepared from specific chitosanase, the catalytic activity is high, and the method can be carried out under a high-temperature condition. The chitosanase disclosed by the invention can be used for carrying out enzymolysis on chitosan under a high-temperature condition, and chitosan oligosaccharide is prepared by efficiently carrying out enzymolysis on the chitosan.

The invention relates to the biotechnical field and particularly discloses a preparation method of fish protein peptide. The method comprises steps as follows: raw material preparation: raw materialscomprise low-value small trash fish in seas and aquatic product leftovers; raw material crushing: fish paste enzymolysis: fish paste in the crushing step is transferred into an enzymolysis tank, wateris added to the enzymolysis tank, and the water and the fish paste are mixed in the mass ratio being 1:1 to form a mixture A, wherein the enzymolysis temperature is 50-70 DEG C, the pH during enzymolysis is 7.0 and the enzymolysis lasts for 2-6 h; enzyme deactivation; small peptide liquid preparation; enzymatic hydrolysate spray drying; microbial fermentation: aspergillus oryzae is mixed in a solid in the small peptide liquid preparation step to form a mixture C, the water content of the mixture C is 30%-60%, the temperature is 25-45 DEG C, fermentation time is 16-28 h, and a mixture D is formed; drying; mixing and crushing. By means of the scheme, protein peptide can be extracted from low-value small trash fish and aquatic product leftovers, and thus, the processing cost of the protein peptide is reduced.

The invention provides a cassava residue fermented feed and a preparation method thereof, and belongs to the technical field of utilization of cassava residues. The cassava residue fermented feed is prepared by mixing and fermenting the following raw materials in parts by weight: 240 to 260 parts of cassava residue, 20 to 25 parts of wheat bran, 15 to 20 parts of cottonseed cake, 10 to 15 parts offructus momordicae, 15 to 20 parts of beet, 5 to 8 parts of fish meal, 3 to 5 parts of shell powder, 8 to 10 parts of herba trifolii repens, 0.8 to 1.5 parts of fructus corni, and 0.06 to 0.08 part of active enzyme. The preparation method of the cassava residue fermented feed has the advantages that by utilizing the fermenting and enzyme hydrolysis function, the utilization conversion rate of thecassava residue is improved; the preparation method is simple and is suitable for large-scale application; multiple beneficial components of cellulose, saccharides and the like are contained, and canbe easily digested and absorbed.

The invention discloses a method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and a pretreated solid matrix, comprising the following steps: carrying out prehydrolysis on degreased peanut powder, including ball-milling treatment, enzyme hydrolysis, separation and other processes; then, mixing the degreased peanut powder prehydrolysate with the pretreated solid matrix, and carrying out ultrasonic treatment; and finally, adding the pretreated solid matrix, cellulase, cellobiase and yeast in batches, and carrying out semi-synchronous enzymolysis and fermentation on the degreased peanut powder prehydrolysate and the pretreated solid matrix to produce the fuel ethanol. According to the invention, peanut protein, monosaccharideor oligosaccharide is extracted from cheap degreased peanut powder; peanut protein can effectively shield lignin deposited on the surface of the pretreated solid matrix and reduce the use amount of cellulase, monosaccharide or oligosaccharide can improve the concentration of fermentable sugar and the concentration of the fuel ethanol in fermentation liquor, and the purpose of effectively reducingthe production cost of the fuel ethanol is achieved.

The invention relates to the technical field of biology, in particular to instant milk powder contributing to digestion and absorption and a preparation process thereof. According to the preparation process, milk protein concentration is firstly hydrolyzed into peptide, thereby reducing the viscosity of feed liquid, and facilitating working procedures such as pipeline transportation and later-period desugarization and enzyme deactivation; meanwhile, digestion and absorption are easier, and sensitization is reduced. A product provided by the invention contains OPO with the chemical name of 1,3-dioleoyl-2-palmitoyl triglyceride, so that the product is more approximate to breast milk, and has remarkable effects of reducing constipation and defecation difficulty of babies, reducing loss of nutritional ingredients and promoting skeletal development of the babies. Meanwhile, the invention relates to the production process of the milk powder, and solves the problem of difficult brewing of milk powder due to the existence of fine powder; the fine powder can not only be recycled, but also be recycled for granulation.

The invention discloses an ionic liquid-complex microbial inoculum system which comprises ionic liquid and a complex microbial inoculum. The ionic liquid and the complex microbial inoculum form a coupling system for enzymolysis of lignocellulose; the ionic liquid contains 1-ethyl-3-methylimidazole ferric chloride salt; and the complex microbial inoculum comprises fermentation liquor of wet cellulomonas, streptomyces albus, candida tropicalis and white rot fungi. In an enzymolysis test, the highest cellulase activity of enzymatic hydrolysate is 185.1 U.mL<1>, the degradation weight loss rate ofgarden biomass reaches 52.6% and is respectively increased by 59.0% and 66.5% with respect to that of a only complex microbial inoculum, and lignin content of enzymolysis residues is reduced by 35.2%. In the enzymolysis application, the enzymolysis period of the garden biomass is 18 to 26 days, the high-temperature period lasts for 12 to 21 days, the highest temperature reaches 62 to 72 DEG C, the final degradation weight loss rate is 47.2 to 54.2%, and the lignin content is reduced to 8.5 to 10.6%. The ionic liquid-complex microbial inoculum system is simple and convenient in preparation process and outstanding in enzymolysis efficiency, and can meet the social requirements of intensive and resource treatment and utilization of agriculture and forestry biomass and the development conceptof green circulation.

The invention provides a preparation method of a sea cucumber beauty health-care product, and belongs to the technical field of health-care products, and the preparation method comprises the following steps: S1, washing and soaking fresh sea cucumbers; S2, performing grinding treatment to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile reaction kettle, adding soda water and anthocyanin, and performing refrigeration; S3, adding xylanase and protease, stirring, heating, and carrying out an enzymatic hydrolysis reaction; S4, respectively grinding barley, peach gum and pummelo peel into powder; baking and frying brown rice with slow fire, and grinding into brown rice slurry; and S5, adding barley, peach gum, pummelo peel powder and brown rice slurry into a reaction kettle, then adding the peach gum and pummelo peel which are ground into powder, homogenizing, discharging and filling to obtain a finished product. The sea cucumber protein can be hydrolyzed to a high degree under the relatively mild condition, active ingredients are reserved as many as possible, and the effects of beautifying and health care are achieved.