Preparation method of fish protein peptide

Abstract

The invention relates to the biotechnical field and particularly discloses a preparation method of fish protein peptide. The method comprises steps as follows: raw material preparation: raw materialscomprise low-value small trash fish in seas and aquatic product leftovers; raw material crushing: fish paste enzymolysis: fish paste in the crushing step is transferred into an enzymolysis tank, wateris added to the enzymolysis tank, and the water and the fish paste are mixed in the mass ratio being 1:1 to form a mixture A, wherein the enzymolysis temperature is 50-70 DEG C, the pH during enzymolysis is 7.0 and the enzymolysis lasts for 2-6 h; enzyme deactivation; small peptide liquid preparation; enzymatic hydrolysate spray drying; microbial fermentation: aspergillus oryzae is mixed in a solid in the small peptide liquid preparation step to form a mixture C, the water content of the mixture C is 30%-60%, the temperature is 25-45 DEG C, fermentation time is 16-28 h, and a mixture D is formed; drying; mixing and crushing. By means of the scheme, protein peptide can be extracted from low-value small trash fish and aquatic product leftovers, and thus, the processing cost of the protein peptide is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing fish protein peptides. Background technique [0002] Due to the rapid development of aquaculture and special breeding in China, the demand for animal protein feed is growing rapidly. Animal protein feed has the advantages of high protein content, high animal meat yield, and easy matching with other ingredients. However, the fish meal produced in my country is generally high-grade. The price of fishmeal is relatively low, and the processing technology is backward, resulting in the low price of fishmeal. Some fishmeal with high protein content, such as special meal for prawns and special meal for soft-shelled turtle, have a serious problem of relying on imports. [0003] At present, the main economic fish resources in the ocean are withered, and the low-value small trash fish are insufficiently caught and processed due to their various shortcomings. L...

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods for preparing bioactive peptides, the common ones are enzymatic hydrolysis and microbial methods, but due to the high cost of external enzymes in the enzymatic hydrolysis method, the enzymatic hydrolysis time for preparing small peptides by the microbial method is too long, the cost is high, and conventional methods are used. After protease enzymatic hydrolysis or microbial hydrolysis, the amount of protein hydrolyzed in raw materials can only reach 26%, and the content of polypeptides below 3000Da is only 83%. This hydrolysis efficiency and degree of hydrolysis are difficult to achieve industrial production.

Images