352 results about "Aspergillus oryzae" patented technology

The invention relates to the field of microorganisms, and discloses an Aspergillus oryzae strain and application thereof.The Aspergillus oryzae can produce protease, aminopeptidase, glutaminase, cellulase, amylase and pectinase at high yield, and when the Aspergillus oryzae is applied to soy sauce brewing, the utilization rate of raw materials can be increased, the content of amino acid in soy sauce can be increased, and the flavor of the soy sauce can be increased.

The invention discloses a compound feed for raising plateau live pigs and a preparation method thereof, and relates to the technical field of feed processing. The compound feed for raising the plateau live pigs is prepared from manganese-selenium compound modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles, aspergillus oryzae, soybean meal powder, corn flour, wheat bran, mountain flour, calcium hydrophosphate, table salt and multivitamins. Manganese-selenium composite modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles and aspergillus oryzae are subjected to co-fermentation culture to form a uniform, stable, loose and porous three-dimensional network fermentation substrate composite system, so that the damage of gastric acid to components is reduced, the targeted release of intestinal tracts is realized, and the bioavailability is improved. The composite functional additive which is high in nutrition, easy to absorb, anti-oxidation, anti-anemia, anti-ultraviolet radiation and the like is obtained, and the adverse effects of low temperature, oxygen deficit and ultraviolet factors on animal growth and feed oxidative rancidity are effectively reduced.

The invention discloses antioxidant salmon collagen peptide and a preparation method thereof, and belongs to the technical field of processing of salmon collagen peptide. The preparation method comprises the following steps: firstly, carrying out enzymolysis on the salmon material by using phospholipase A2, hydrolyzing abundant membrane phospholipids in the salmon material, destroying fat cell structures and releasing wrapped triglyceride, then carrying out secondary enzymolysis by using aspergillus oryzae lipase, hydrolyzing macromolecular lipids into free fatty acids which are easily dissolved in supercritical carbon dioxide, and carrying out secondary enzymolysis by using aspergillus oryzae lipase. The degreased salmon material is obtained by means of supercritical carbon dioxide extraction, the lipid removal rate is greatly increased, and the antioxidant salmon collagen peptide which is small in molecular weight and high in oxidation resistance is obtained through subsequent collagen extraction and enzymolysis.

The invention relates to the technical field of microorganisms, in particular to an Aspergillus oryzae ZA307 strain and application of the Aspergillus oryzae ZA307 strain. The aspergillus oryzae ZA307 high-yield protease and aminopeptidase provided by the invention cooperate with aspergillus oryzae Huniang 3.042 for starter propagation, so that the activity of the protease, aminopeptidase, saccharifying enzyme and amylase of a starter material can be remarkably improved, and furthermore, the content of ammonia nitrogen and total nitrogen and the variety and content of volatile flavor substances in a product are remarkably improved. The soy sauce or soybean paste prepared and fermented by double-strain starter propagation is obviously improved in the aspects of sauce fragrance, ester fragrance, delicate flavor and harmonious taste, and the quality of the product is comprehensively improved.

The invention discloses a decay-promoting and carbon-retaining agent and a preparation method, application and a verification method thereof. The decay-promoting and carbon-retaining agent comprises a complex microbial inoculant and a nutrient preparation, the complex microbial inoculant comprises the following substances in parts by weight: 2 parts of aspergillus oryzae, 4 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 2 parts of lactic acid bacteria, 4 parts of Koxime saccharomycetes, 3 parts of acetobacter aceti, 4 parts of aspergillus candidus, 1 part of deuteromycetes and 1 part of arthrobacter; the nutrient preparation comprises the following substances in parts by weight: 5 parts of urea, 2 parts of diammonium phosphate and 1.3 parts of potassium sulfate. According to the method, the method for verifying the effect of mixed application of the straw and the decay-promoting and carbon-retaining agent to the soil is constructed, and the 13C isotope tracing technology is combined to verify the SOC immobilization effect of the decay-promoting and carbon-retaining agent on the soil with different textures, so that the situation that the decay-promoting and carbon-retaining agent with different components can achieve more effective SOC immobilization on the soil with the different textures is obtained, and straw resources are fully utilized.

The invention discloses a high-efficiency enzymatic fermentation soy sauce production process, and relates to the technical field of soy sauce production processes, the high-efficiency enzymatic fermentation soy sauce production process comprises the following process steps: S1, material preparation, S2, material steaming, S3, inoculation, S4, fermented grain preparation and fermentation, S5, squeezing, and S6, sterilization and filling. The high-temperature-resistant amylase is added in the material steaming process, starch on the surfaces of the wheat and soybean meal raw materials is preliminarily decomposed, the starchy raw material can be decomposed in advance, growth of the aspergillus oryzae raw material in starter propagation is facilitated, clinker can be prevented from caking and being sticky, and the looseness of the starter material is improved; papain is added in the starter propagation process, and protein in wheat and soybean meal raw materials is continuously decomposed by aspergillus oryzae protease in starter propagation, so that the protein raw materials are quickly decomposed in advance, the growth of the aspergillus oryzae raw materials in starter propagation is facilitated, and the fermentation period is shortened; the compound clarification enzyme is added during fermentation to decompose intermediate products such as phytic acid, cellulose and the like which cause a large amount of aggregation after the raw soy sauce is heated, the clarification speed is high after the soy sauce is sterilized, and the precipitation amount is reduced by 50%-70%.

The invention relates to a preparation method of a double-bacterium fermented opuntia ficus-indica fermentation extract and application of the double-bacterium fermented opuntia ficus-indica fermentation extract in cosmetics, and belongs to the technical field of cosmetics. The invention provides a preparation method of a double-bacterium fermented opuntia ficus-indica fermentation extract and application of the double-bacterium fermented opuntia ficus-indica fermentation extract to cosmetics, and the preparation method roughly comprises the following steps: preparing opuntia ficus-indica stem slurry and rice milk, mixing the slurry and the rice milk, performing double-bacterium fermentation, centrifuging, retaining residues, and finally enriching supernate. According to the invention, food-grade opuntia ficus stem and rice are used for fermentation, and aspergillus oryzae and saccharomycetes are used for combined fermentation, so that the introduction of exogenous enzyme is avoided, macromolecular substances are fully decomposed through synergistic metabolism, the release rate of micromolecular active ingredients is increased, and finally, the effects of moisturizing, oxidation resistance, wrinkle resistance and the like of the product are further improved through enrichment.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention discloses efficient expression of an aspergillus oryzae beta-galactosidase mutant and application of the aspergillus oryzae beta-galactosidase mutant in dairy products. The invention belongs to the technical field of enzyme engineering and the technical field of dairy product processing, and particularly relates to efficient expression of an aspergillus oryzae beta-galactosidase mutant and application of the aspergillus oryzae beta-galactosidase mutant in dairy products. The mutant protein disclosed by the invention can be as follows: A1) a protein with an amino acid sequence of SEQ ID No.2; a2) a fusion protein which is obtained by connecting a label to the N end and / or the C end of A1) and has the same function. The mutant protein is subjected to high-density fermentation in a 5L fermentation tank, the enzyme activity of fermentation liquor can reach 4628U / mL, and the recovery rate of mutant enzyme is increased by 1.7 times. Compared with a wild type, the mutant has the advantages that the optimum pH is increased from 4.5 to 5.5, the optimum temperature is reduced from 60 DEG C to 50 DEG C, the lactose hydrolysis efficiency is improved by 11%, and the mutant is more suitable for a production process of low / zero lactose dairy products.

The preparation method comprises the following steps: mixing salvia miltiorrhiza-schisandra chinensis superfine powder and roxburgh rose normal juice in a ratio of 3: 2: 5, carrying out composite enzymolysis and saccharomyces cerevisiae pretreatment, and sequentially carrying out aspergillus oryzae solid-state fermentation, acetobacter pasteurianus liquid-state index fed-batch fermentation and lactobacillus plantarum anaerobic flavor fermentation, so as to obtain the vinegar product with the homology of medicine and food. And parameters are regulated and controlled in real time through the three-layer stacked LSTM neural network. According to the invention, the conversion rate of tanshinone IIA to tanshinol reaches 65.8%, the total acid is greater than or equal to 7.2 g / 100mL, at least 21 flavor substances are contained, the batch difference rate is 4.2%, the energy consumption is reduced by 29.2%, the equipment utilization rate is increased by 50%, the product safety reaches the standard, and the method has a health-care function and palatability, and is suitable for industrial production.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention relates to the technical field of food processing, and particularly discloses soybean flavor peptide and a preparation method and application thereof. Aspergillus oryzae is selected as a leavening agent, a microbial endogenous enzyme generated by full fermentation of the aspergillus oryzae is utilized to cooperate with a compound enzyme preparation to perform combined action, abundant enzyme systems effectively perform enzymolysis on macromolecules such as protein, and related taste substances are provided for subsequent enzymolysis products; in the soybean flavor peptide prepared by enzyme hydrolysis and Maillard reaction, the contents of target peptide and target amino acid (umami peptide, umami amino acid and the like) are high; when the soybean flavor peptide is applied to the compound seasoning with the main function of increasing freshness, the freshness score can be increased by 0.5-2 times. In addition, on the basis of improving freshness, the taste richness of the food or the compound seasoning can be enhanced, and the aftertaste time is prolonged.

The invention relates to a composition for promoting collagen generation based on sophora flower powder as well as a preparation method and application of the composition, and belongs to the technical field of sophora flower extracts. A preparation method of the sophora flower powder extract comprises the following steps: taking sophora flower powder, adding water, boiling, cooling, adding a carbon source and aspergillus oryzae CICC 2066, fermenting, sterilizing, filtering, concentrating and freeze-drying to obtain the sophora flower powder extract. The sophora flower powder extract can be applied to food, can also be applied to cosmetics, and can effectively promote the generation of collagen and improve the skin elasticity and tightening effect.

An application of rice NAT protein and a coding gene thereof in regulation and control of plant disease resistance, a method for regulating and controlling rice resistance to rice blast and a method for cultivating transgenic rice with high resistance to rice blast are provided. In the research of rice immune response, a gene NAT of which the translation is induced by M. oryzae is identified and obtained.

The invention provides high-satiety chicken small molecule peptide and application thereof in chicken meal replacement powder.The method comprises the steps that firstly, intragastric self-swelling microspheres are prepared, wall materials are yeast beta-glucan and hydroxypropyl methyl cellulose, a core material is modified chicken small molecule peptide, the microspheres absorb water to swell in a gastric acid environment to generate satiety, and the self-swelling microspheres absorb water to swell in the gastric acid environment; the modified chicken small molecule peptide is slowly released in an intestinal environment, so that absorption is promoted, and intestinal health is regulated; in addition, the coarse cereal powder is subjected to solid state fermentation by using aspergillus oryzae and then is used for preparing the chicken meal replacement powder, and the self-expanding microspheres in the stomach and the fermented coarse cereal powder are added, so that the satiety is improved, and the market demand of healthy meal replacement food is met.

The application discloses efficient expression of Aspergillus oryzae beta-galactoside enzyme mutant and application thereof in dairy products, and belongs to the technical fields of enzyme engineering and dairy product processing, and particularly relates to efficient expression of Aspergillus oryzae beta-galactoside enzyme mutant and application thereof in dairy products.The mutant protein can be as follows: A1) a protein with an amino acid sequence of SEQ ID No.2; A2) a fusion protein with the same function obtained by connecting a label to the N terminal and / or C terminal of A1).The mutant protein is subjected to high-density fermentation in a 5L fermentation tank, and the enzyme activity of the fermentation liquor can reach 4628U / mL, and the recovery rate of the mutant enzyme is increased by 1.7 times.Compared with the wild type, the optimal pH of the mutant is increased from 4.5 to 5.5, the optimal temperature is decreased from 60 DEG C to 50 DEG C, the lactose hydrolysis efficiency is increased by 11%, and the mutant is more suitable for the production process of low / zero lactose dairy products.

The utility model discloses an aspergillus oryzae fermentation extraction device, which belongs to the technical field of fermentation devices and comprises a water tank, an electric heating box fixedly connected to the top end of the water tank and a mixing box fixedly connected to the top end of the electric heating box, a steam box is arranged above the mixing box, and a pressing block is arranged above the steam box. According to the utility model, the electric heating box, the mixing box, the steaming box and the pressing block are matched with one another, so that the processes of steaming, mixing and fermenting rice can be realized, the rice does not need to be transferred for many times, and the efficiency from steaming to fermenting of the rice is obviously improved; according to the rice steaming machine, when the rice is steamed, the steamed rice is driven to move upwards, the rice penetrates through a net plate under the downward pressing action of a pressing block, the rice can be cut up under the cutting action of the net plate, and meanwhile the mixing difficulty of the rice and aspergillus oryzae strains can be remarkably reduced under the mixing action of a comb plate.

The invention relates to the technical field of microbial fermentation, and particularly discloses a microbial fermentation process for food processing. The composition used in the process comprises lactobacillus plantarum, aspergillus oryzae and a compound enzyme preparation containing neutral protease, cellulase and phytase. The preparation method comprises the following steps: firstly, pretreating the raw materials, then compounding lactobacillus plantarum and aspergillus oryzae into a fermentation starter according to a specific proportion, fermenting in a liquid environment, and implementing dynamic stepped oxygen control; adding a complex enzyme preparation in a hypha growth period; then transferring into solid matrix conversion to construct a porous structure; and finally, carrying out two-stage temperature-controlled vacuum drying to obtain the product. The composition disclosed by the invention can be used for fermenting plant-based raw materials such as beans and grains, and has the advantage that the availability of a substrate can be improved through the synergistic interaction of bacteria and enzymes; in addition, according to the process, through stepped oxygen control, the double-bacterium respiratory rhythm can be precisely adapted, and the problem of metabolism mutual exclusion in a constant oxygen mode is thoroughly solved.

To provide a novel technique which enables efficient production of mycelium in a short time.SOLUTION: The present invention provides a method for producing mycelium of koji mold, the method including a culture step involving liquid-culture of koji mold in a culture medium containing grains, and a recovery step involving recovery of the mycelium obtained in the culturing step.SELECTED DRAWING: None

The invention discloses an aspergillus oryzae engineering bacterium as well as a construction method and application of the aspergillus oryzae engineering bacterium, and relates to the technical field of genetic engineering. The method comprises the following steps of: (a) connecting and fusing an open reading frame of an endogenous gene with a fungal laccase copper oxidoreductase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; or connecting and fusing an open reading frame of an endogenous gene with a multi-copper oxidase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; and (b) at least one of a gene Aovps10, a gene AosedD and a gene Aoemp47 is knocked out. The aspergillus oryzae engineering bacterium provided by the invention can realize high-efficiency expression of the heterologous protein, and the extracellular yield of the heterologous protein is obviously improved.

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention provides a beef essence preparation method and system based on dual enzymolysis-Maillard reaction synergistic interaction. The preparation method comprises the following steps: (1) carrying out first-step enzymolysis, namely adding compound protease into minced beef, regulating the pH value of a system to 6.5-7.0, controlling the temperature to be 45-50 DEG C, and carrying out enzymolysis reaction for 2-3 hours; (2) carrying out second-step enzymolysis, adding lipase derived from aspergillus oryzae, adjusting the pH value of the reaction system to 7.5-8.0, raising the temperature to 55-60 DEG C, and carrying out enzymolysis reaction for 1-2 hours; (3) performing enzyme deactivation treatment to obtain enzymatic hydrolysate; and (4) mixing the enzymatic hydrolysate with reducing sugar, thiamine and a yeast extract, and carrying out a Maillard reaction. The beef ingredients can be fully decomposed through double enzymolysis, and sufficient flavor precursor substances are provided for reaction; the Maillard reaction is intelligently regulated and controlled, more flavor substances are generated, and the flavor of the essence is remarkably improved.

The invention discloses a soothing and repairing gentiana macrophylla extract composition as well as a preparation method and application thereof, and particularly belongs to the technical field of preparation of raw materials of daily necessities. The gentiana macrophylla extract composition is obtained by performing compound solid-state fermentation on gentiana macrophylla through aspergillus oryzae, leuconostoc citreum and saccharomyces cerevisiae and then performing solvent extraction. The gentiana macrophylla extract composition provided by the invention has relatively high oleanolic acid content, which proves that the aspergillus oryzae, the leuconostoc citreum and the saccharomyces cerevisiae can effectively enrich active ingredients in gentiana macrophylla, and meanwhile, the aspergillus oryzae, the leuconostoc citreum and the saccharomyces cerevisiae have a certain synergistic effect on the enrichment of the active ingredients in the fermentation process; the gentiana macrophylla extract composition provided by the invention can effectively inhibit release of inflammatory factors and promote skin repair.

The invention discloses a method for improving livestock and poultry breeding wastewater treatment by a phycomycete system through starch wastewater and application, and belongs to the technical field of environmental engineering and biological recycling. The method comprises the following steps: (1) pretreating livestock and poultry breeding wastewater and starch wastewater to remove impurities; livestock and poultry breeding wastewater and starch wastewater are mixed according to a certain volume ratio to prepare mixed wastewater; (2) mixing chlorella sorojin and aspergillus oryzae in a logarithmic phase according to a certain quantity ratio, and inoculating to construct a microalgae-fungus co-culture system; and (3) putting the microalgae-fungus co-culture system into the mixed wastewater, treating under an illumination oscillation condition, and purifying the mixed wastewater. According to the method, the starch wastewater is adopted as a novel carbon source for replacing a traditional commercial carbon source, and a biological treatment mode of microalgae-fungus co-culture is combined, so that efficient purification of the livestock and poultry breeding wastewater is realized, meanwhile, biomass accumulation is promoted, resource utilization of wastes is realized, and dual targets of wastewater treatment and resource recovery are achieved.

The invention relates to the technical field of microorganisms, in particular to soybean paste and a preparation method thereof. The soybean paste comprises the following raw material components: soybeans, flour, aspergillus oryzae, saline water, xanthan gum, pre-gelatinized wheat starch, vegetable oil and saccharomyces cerevisiae. The saccharomyces cerevisiae is screened and separated from commercially available soybean paste, and is preserved in the China Center for Type Culture Collection on November 13, 2024, and the preservation number is CCTCC NO: M 20242550. Compared with the traditional soybean paste obtained by natural fermentation, the soybean paste disclosed by the invention has similar volatile flavor substances, but the succinic acid content is obviously increased, so that the soybean paste shows better taste. The screened and separated saccharomyces cerevisiae is independently inoculated for fermentation to generate rich flavor substances, so that the risk of infectious microbe pollution in the fermentation process can be reduced, the fermentation time is shortened, and the quality and production efficiency of the soybean paste are synchronously improved.

The invention relates to a microbial agent based on a phosphate tailing storage yard and application of the microbial agent, the microbial agent based on the phosphate tailing storage yard comprises a talaromyces new species Talaromyces coriarcola KY-QH135, which is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753, and the microbial agent based on the phosphate tailing storage yard is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753. The invention relates to the field of biotechnology, and in particular relates to a biotechnology which is selected from one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger, as well as one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger.

The invention discloses a Chinese herbal medicine leavening, a preparation method thereof and application of the Chinese herbal medicine leavening in cosmetics, and particularly relates to the technical field of cosmetics. Comprising the following raw materials in parts by weight: 12-18 parts of radix astragali, 8-12 parts of radix ampelopsis, 10-15 parts of radix saposhnikoviae, 18-22 parts of rhizoma chuanxiong, 6-9 parts of bitter almond seeds, 18-22 parts of radix salviae miltiorrhizae, 7-10 parts of herba centellae, 6-9 parts of folium artemisiae argyi, 7-10 parts of fructus gardeniae, 4-6 parts of flos sophorae immaturus, 5-8 parts of fructus citri, 12-15 parts of poria cocos and 1.5-2.5 parts of a dendrobium officinale extract. The composition can synergistically exert moisturizing, immune regulation and anti-allergy effects with other raw material components to form an anti-allergy, anti-oxidation and repair efficacy closed loop, a process chain from aspergillus oryzae high-enzyme-activity solid fermentation to double-bacterium liquid symbiosis to chitinase directional enzymolysis can synergistically improve the yield of active matters, and the situation that traditional anti-allergy components have no hair growth effect is broken through, so that the anti-allergy and anti-allergy effects are achieved. Due to the limitation that the hair restorer stimulates sensitive skin, the hair growing and double effects are integrated, and the hair restorer is suitable for preparing cosmetics for resisting allergy, resisting oxidation and repairing barriers and is especially suitable for sensitive skin care and scalp care.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

The invention provides a low-sensitization whey protein hydrolysate as well as a preparation method and application thereof, and the preparation method of the low-sensitization whey protein hydrolysate comprises the following steps: adjusting the pH value of whey liquid to be alkaline, then adding protease, and carrying out heat-preservation hydrolysis, the protease is selected from at least one of bacillus licheniformis alkaline protease, aspergillus oryzae neutral protease and aspergillus mellitus protease. At least one of the bacillus licheniformis alkaline protease, the aspergillus oryzae neutral protease and the honey aspergillus protease is adopted to hydrolyze the whey liquid, unique active ingredients of protein hydrolysate can be enriched, and therefore the problem that existing protein hydrolysate is high in sensitization is effectively solved, excessive hydrolysis is avoided, and the product quality is improved. The nutritional value of protein is reserved, the crowd acceptability is high, the problem that special crowds are allergic or intolerant to cow milk protein is effectively solved, the method is simple, the raw materials are easy to obtain, and the cost is low.