Preparation method of compound microbial feed additive

Abstract

The invention relates to a feed additive, in particular to a prepration method of a compound microbial feed additive, which is used for solving the problems that a variety of feed additives have respective different shortcomings in the actual applications. The preparation method comprises the following steps: preparing selenium yeast, iron yeast and zinc yeast which are rich in trace elements, taking vinegar residue, wheat bran, corn flour, compound Daqu, ammonium sulfate and other auxiliary materials as culture media, adding aspergillus niger, aspergillus oryzae, bacillus subtilis, lactobacillus and yeast, producing a multi-bacterial compound enzyme by the solid-state method, and mixing the functional yeast, phytase and the multi-bacterial compound enzyme for producing a biological feed micro-multi-enzyme which has a large number of live bacteria in beneficial bacterial groups, a complete enzyme system of digestive enzymes and non-digestive enzymes needed by animals and high enzyme activity, and is rich in organic trace elements, vitamins, amino acids and unknown growth-promoting factors with functional nutrition for the animals and people; furthermore, the feed additive has the advantages of advanced process, abundant nutrition, abundant bacterial groups, full enzyme spectrum, high activity, greenness, no pollution and the like.

Description

technical field

[0001] The invention relates to a feed additive, in particular to a preparation method of a compound micro-ecological feed additive. Background technique

[0002] With the development of the economy and the improvement of people's living standards, safe, hygienic, pollution-free and green livestock and poultry products will be favored by consumers, and animal food has better quality and higher safety. Towards. The relationship between animal feed and livestock products is very close. The former is directly related to the quality of the latter, and the quality of livestock and poultry products is directly related to human health. Animal feed is transformed into livestock and poultry products for human consumption, which forms a food chain such as animal feed-livestock and poultry products-human food. Therefore, the safety of animal feed is directly related to people's health and life safety. At the same time, how to improve the utilization of feed High effic...

Examples

Embodiment 1

[0039] A preparation method of a compound microecological feed additive, comprising the following steps:

[0040] (1) Preparation of organic functional yeast rich in trace elements:

[0041] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 20 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 3% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 18h, add the sodium selenite solution of 3mg / L, continue to cultivate to 45h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0042] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...

Embodiment 2

[0061] A preparation method of a compound microecological feed additive, comprising the following steps:

[0062] (1) Preparation of organic functional yeast rich in trace elements:

[0063] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 25 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 6% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 25h, add the sodium selenite solution of 6mg / L, continue to cultivate to 55h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0064] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...

Embodiment 3

[0083] A preparation method of a compound microecological feed additive, comprising the following steps:

[0084] (1) Preparation of organic functional yeast rich in trace elements:

[0085] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 23 hours as seed liquid; prepare pH4. 5 YEPD liquid medium, inoculate activated beer yeast with 5% bacterial weight, and after 20 hours of reciprocating shaking culture on a shaker at 30 ° C, add 5 mg / L sodium selenite solution and continue to cultivate until 48 hours; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0086] B. Preparation of iron yeast: Passage beer yeast on a YEPD slant to obtain activated strains, inoculate the activated strains in the prepared YEPD liquid medium, and shake at 28°C for 22 ho...

technical field

[0001] The invention relates to a feed additive, in particular to a preparation method of a compound micro-ecological feed additive. Background technique

[0002] With the development of the economy and the improvement of people's living standards, safe, hygienic, pollution-free and green livestock and poultry products will be favored by consumers, and animal food has better quality and higher safety. Towards. The relationship between animal feed and livestock products is very close. The former is directly related to the quality of the latter, and the quality of livestock and poultry products is directly related to human health. Animal feed is transformed into livestock and poultry products for human consumption, which forms a food chain such as animal feed-livestock and poultry products-human food. Therefore, the safety of animal feed is directly related to people's health and life safety. At the same time, how to improve the utilization of feed High effic...

Examples

Embodiment 1

[0039] A preparation method of a compound microecological feed additive, comprising the following steps:

[0040] (1) Preparation of organic functional yeast rich in trace elements:

[0041] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 20 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 3% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 18h, add the sodium selenite solution of 3mg / L, continue to cultivate to 45h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0042] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...

Embodiment 2

[0061] A preparation method of a compound microecological feed additive, comprising the following steps:

[0062] (1) Preparation of organic functional yeast rich in trace elements:

[0063] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 25 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 6% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 25h, add the sodium selenite solution of 6mg / L, continue to cultivate to 55h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0064] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...

Embodiment 3

[0083] A preparation method of a compound microecological feed additive, comprising the following steps:

[0084] (1) Preparation of organic functional yeast rich in trace elements:

[0085] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 23 hours as seed liquid; prepare pH4. 5 YEPD liquid medium, inoculate activated beer yeast with 5% bacterial weight, and after 20 hours of reciprocating shaking culture on a shaker at 30 ° C, add 5 mg / L sodium selenite solution and continue to cultivate until 48 hours; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;

[0086] B. Preparation of iron yeast: Passage beer yeast on a YEPD slant to obtain activated strains, inoculate the activated strains in the prepared YEPD liquid medium, and shake at 28°C for 22 ho...