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Magnetic bead-based sample treatment and nucleic acid automatic extraction system

The invention discloses a magnetic bead-based sample treatment and nucleic acid automatic extraction system, comprising a micro-fluidic chip and a micro motor drive device, wherein the micro-fluidic chip comprises a layer of cover plate, two layers of bottom plates and two layers of structure plates, wherein the chip can automatically finish the operation processes such as cell lysis, nucleic acid purification, nucleic acid eluting and the like, and finally a nucleic acid sample is obtained. Based on siphon principle or micro-fluidic surface tension properties, a driven drive pump without external drive force is designed by the chip, so as to achieve flowing and transferring of a detection sample, a reaction reagent and reaction liquid waste. Under mixed hardening of a micro vibration motor, through a periodic alternating magnetic field and a constant magnetic field provided by an external magnet, effective mixing between the magnetic bead and the reaction sample and the reaction reagent in a reaction cavity, and fixing of the magnetic bead in the liquid waste discharge process are achieved; and the chip is matched with a full-automatic micro motor drive device to automatically finish a plurality of reaction steps, so that a high-purity nucleic acid sample template is provided for gene amplification reaction.
Owner:BEIJING UNIV OF CHEM TECH +1
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