5094results about "Flow mixers" patented technology

An external mixer assembly is provided which externally mixes and delivers a first and a second component of a biological adhesive to tissues or organs for sealing wounds, stopping bleeding and the like. The first and second components are mixed immediately after exiting from separate outlet ports disposed in fluid communication with component reservoirs. In on embodiment, the external mixer assembly includes a housing having a housing head for enclosing therein a first reservoir containing the first component, and a second reservoir containing the second component. The housing further includes a discharge nozzle defining a longitudinal axis for enclosing therein a conduit assembly having a first and a second conduit in communication with the first and second reservoir, respectively. A deflector assembly is connected to the discharge nozzle. The deflector assembly includes a deflector plate to provide a space for initial mixing of the first and second components. The deflector plate is oriented in generally parallel juxtaposed relation distal to the distal face of the discharge nozzle. The first and second components are preferably fibrinogen and thrombin which intermix to form a fibrin sealant.

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

A conductorless charging and power system for electronic appliances and method for communicating power to a power receiver employing wireless energy transmission are disclosed. The remote charging system includes a power transmission unit, which transmits energy as a directional power beam, and a power receiver system that receives the transmitted energy. The power receiver system is preferably incorporated in an appliance and includes an energy receptor capable of receiving the wireless power beam and transferring the energy from the beam to an energy storage device included in the appliance. The power transmission unit receives and tracks a power request signal from the power receiver system to track the power receiver system location during energy transmission. Data streams may be incorporated into the wireless signals of the remote charging system, allowing the remote charging system to function as a communications pathway as well as a power delivery system.

Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalising the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.

A method of making high internal phase emulsions is described. The method forms high internal phase emulsion (HIPE) using a single pass through the static mixer. In alternative embodiments, the HIPE may be further processed to farther modify the size of dispersed phase droplets, to incorporate additional materials into the HIPE, to alter emulsion temperature, and the like.

A counterflow mixing device for a process chamber is disclosed, comprising an injection tube that introduces a fluid in a manner counter to a flow of a post-plasma gas mixture traveling downward from a plasma source. The invention allows for proper mixing of the fluid as well as avoiding recombination of generated ions and radicals.

Methods and implants for treating glaucoma in an eye are described. The implant includes an inlet section configured to be positioned in the anterior chamber of the eye and an outlet section in fluid communication with the inlet section. The outlet section is configured to be positioned in Schlemm's canal of the eye. The implant comprises a hydrogel and is configured to conduct aqueous humor from the anterior chamber to Schlemm's canal.

Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

The movement and mixing of microdroplets through microchannels is described employing microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. Microdroplets are metered into defined volumes and are subsequently incorporated into a variety of biological assays. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.

An apparatus configured for connection to a mixer for receiving a slurry and altering the flow characteristics of the slurry includes a conduit having a main inlet in slurry receiving communication with the mixer outlet and extending to a spout for discharging the slurry, at least one conduit restrictor associated with the conduit for creating back-pressure between the conduit restrictor and the mixer outlet for keeping the mixer full, and at least one pressure reducer associated with the discharge spout and configured for reducing the pressure of the slurry dispensed from the discharge spout. The apparatus is configured for maintaining a generally laminar flow from the mixer outlet to the discharge spout.

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

A series of microactuators for manipulating small quantities of liquids, and methods of using these for manipulating liquids, are disclosed. The microactuators are based on the phenomenon of electrowetting and contain no moving parts. The force acting on the liquid is a potential-dependent gradient of adhesion energy between the liquid and a solid insulating surface.

A mixing fluid providing device (1) which provides with the further homogenized mixed state is provided with a mixing gas tubing (4) that mixes the auxiliary gas provided from the main gas tubing and a mixing fluid homogenizing device (6) that is arranged in the mixing gas tubing (4); the mixing fluid homogenizing device (6) is provided with a fixed perforated plate (8) and a movable perforated plate (9) which are mutually overlapped and jointed, and a driving cylinder (11) which causes the movable perforated plate (9) move in the surface direction relative to the fixing perforated plate (9); each perforated plate (8 and 9) is formed with a plurality of through holes (10) and forms a structure which can change the overlapping degree between the through hole (10) of the two perforated plates (9) and the aperture ratio of all through holes (10) by the moving of the movable perforated plate (9).

The present invention provides structures and methods that allow polymers of any length, including nucleic acids containing entire genomes, to be stretched into a long, linear conformation for further analysis. The present invention also provides structures and methods for selecting and stretching polymers based on their lengths. Polymers are loaded into a device and run through the structures. Stretching is achieved by, e.g., applying shear forces as the polymer passes through the structures, placing obstacles in the path of the polymer, or a combination thereof. Since multiple molecules may be stretched in succession, extremely high throughput screening, e.g., screening of more than one molecule per second, is achieved.

The disclosure relates to a method and an apparatus (1) for continuously mixing two flows, a first, larger flow (2) and a second, smaller flow (3). The second flow (3) is introduced counter-directed into the first flow (2). The apparatus (1) comprises a T pipe (4) where a first connection (6) constitutes an inlet (20) for the first flow. A second connection (7), at 180° in relation to the first connection (6), constitutes an inlet (21) for the second flow (3). The second flow (3) is led into the first flow (2) through a conduit (13) within the T pipe (4). The first connection (6) is provided with a conical portion (10) in which are provided a number of holes (12), so that the first flow (2) is throttled and divided up into a plurality of subflows immediately before the mixing operation. A third connection (9) is oriented at 90° in relation to the other connections (6, 7) and constitutes an outlet (22) for the intermixed flows (19), which implies that the intermixed flows (19) are caused to change direction immediately after the mixing.

Devices and methods for the encapsulation of cells on microfluidic platforms are disclosed. The microfluidic device generally includes a plurality of functional regions to shear, focus, and encapsulate a desired cell or group of cells into a droplet. The microfluidic device can further comprise a polymerization zone to form a polymer bead around the droplet.

Submicron particles of water-insoluble compounds, particularly drugs, are prepared by simultaneously stabilizing microparticulate suspensions of same with surface modifier molecules by rapid expansion into an aqueous medium from a compressed solution of the compound and surface modifiers in a liquefied gas and optionally homogenizing the aqueous suspension thus formed with a high pressure homogenizer.

Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of dense arrays of a variety of fluid control structures, such as structures for isolating, routing, merging, splitting, and storing volumes of fluid. The fluid control structures can be used to implement a pathogen detection and analysis system including integrated immunoaffinity capture and analysis, such as polymerase chain reaction (PCR) and capillary electrophoresis (CE) analysis. An analyte solution can be input into the device and pumped through a series of immunoaffinity capture matrices in microfabricated chambers having antibodies targeted to the various classes of microbiological organisms such as bacteria, viruses and bacterial spores. The immunoaffinity chambers can capture, purify, and concentrate the target for further analysis steps.

A freight container (10) has a base (20) or a portion thereof that can be opened to discharge its contents. This container (10) has a top which can be opened in similar manner as the base. Another container having at least one compartment, each compartment having a lower section with a reducing cross-sectional area and a pivotably operable closure assembly, with several such closure assemblies being linked together by means of bars. Such a container is used to contain raw building materials for stockpiling of these materials at a container port. It is also used to supply materials to a concrete production plant whee pollution control containers are provided below the supply container and above the scaling and mixing stations to reduce particulate pollution. A pair of slewing apparatuses is also provided to engage both ends of a container and turn the container over about its longitudinal axis, thereby emptying its contents.

A microchannel apparatus includes a plural partition walls 4 formed as extensions of plural microchannels 1 toward a continuous phase, and a flow path 5 is formed between adjacent ones of the partition walls 4. A dispersed phase pumped into a continuous phase via each microchannel 1 generates nearly perfect spheres in the course of passing through the microchannel and the flow path 5 in between the partition walls 4, thereby producing emulsions comprising fine and homogenous microspheres.

Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and/or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and/or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.

This invention provides a device and method for creating hydrodynamic cavitation in fluids which includes a flow-through chamber intermediate an inlet opening and an outlet opening; the flow-through chamber having an upstream opening portion communicating with the inlet opening and a downstream opening portion communicating with the outlet opening; the cross-sectional area of the downstream opening portion being greater than the cross-sectional area of the upstream opening portion; and a cavitation generator located within the flow-through chamber for generating a hydrodynamic cavitation field downstream from the generator. This invention also provides for a device for creating hydrodynamic cavitation in fluids wherein the walls of the flow-through chamber are removable mounted within the device and are interchangeable and replaceable with replacement walls having various shapes and configurations, thereby enabling the flow-through chamber to assume various shapes and configurations to affect cavitation. This invention also provides for a device for a device for creating hydrodynamic cavitation in fluids wherein the baffle elements are removably mounted within the device and are interchangeable and replaceable with replacement baffles having various shapes and configurations thereby enabling variable effects on cavitation.

An exhaust aftertreatment system comprises an injector for injecting urea water into an exhaust duct, and a denitration catalyst disposed downstream of the injector with respect to a flow of exhaust gas. The exhaust aftertreatment system reduces nitrogen oxides in the exhaust gas by the denitration catalyst while using ammonia produced from the urea water injected from the injector. The urea water is injected along a direction of the flow of the exhaust gas within the exhaust duct, and a porous plate is disposed in multiple stages in a space of the exhaust duct such that droplets of the injected urea water impinge against the porous plate before reaching a wall surface of the exhaust duct. A surface of the porous plate subjected to the impingement of the droplets is arranged to face downstream with respect to the flow of the exhaust gas. Deposition of the urea water is prevented by causing film boiling when the droplets impinge against the porous plate, and the urea water reflected by the porous plate is uniformly dispersed into the exhaust gas. Thus, the urea water is uniformly dispersed into the exhaust gas without increasing a pressure loss of the exhaust gas. The urea water is prevented from depositing on the wall surface and producing a precipitate in the form of a solid.

A bezel and bezel assembly in which the bezel is a rigid block with a plurality of cavities. A depression in the block has ribs extending up therefrom to form an elevated contour. The depression includes at least one cavity therein for the application of air pressure into the depression and over the elevated contour. A gasket fits over the bezel so that positive pressure applied through the at least one cavity in the depression forces a gasket membrane to expand away from the pumping side and negative pressure applied through the at least one cavity in the depression pulls the gasket membrane against the elevated contour of the ribs. The bezel may include solvent bondable tubing connections for making pneumatic connections to the bezel.

Methods of beverage and flowable food product production using steam injection are provided to efficiently destroy microorganisms able to withstand normal pasteurization temperatures. Microorganisms such as Alicyclobacillus acidoterrestris and its spores may be eliminated from fruit juices and the like while minimizing organoleptic degradation due to heating. The apparatus is capable of pasteurizing, blending and controlling the product specifications of a finished beverage in a continuous matter.