2584results about How to "Rapid production" patented technology

Apparatus for producing an object by sequentially forming thin layers of a construction material one on top of the other responsive to data defining the object, the apparatus comprising: a plurality of printing heads each having a surface formed with a plurality of output orifices and controllable to dispense the construction material through each orifice independently of the other orifices; a shuttle to which the printing heads are mounted; a support surface; and a controller adapted to control the shuttle to move back and forth over the support surface and as the shuttle moves to control the printing heads to dispense the construction material through each of their respective orifices responsive to the data to form a first layer on the support surface and thereafter, sequentially the other layers; wherein each printing head is dismountable from the shuttle and replaceable independently of the other printing heads.

Compositions and methods for modulating the activity of RNA and DNA are disclosed. In accordance with preferred embodiments, antisense compositions are prepared comprising targeting and reactive portions. Reactive portions which act, alternatively, through phosphorodiester bond cleavage, through backbone sugar bond cleavage or through base modification are preferrably employed. Groups which improve the pharmacodynamic and pharmacokinetic properties of the oligonucleotides are also useful in accordance with certain embodiments of this invention. Delivery of the reactive or non-reactive functionalities into the minor groove formed by the hybridization of the composition with the target RNA is also preferrably accomplished. Therapeutics, diagnostics and research methods and also disclosed. Synthetic nucleosides and nucleoside fragments are also provided useful for elaboration of oligonucleotides and oligonucleotide analogs for such purposes.

An inorganic binder and a dissolving agent are put into ceramic powder. They are mixed to form a plastic green mixture. Then the said mixture is formed into a thin green layer. Preferably, this thin green layer will be preheated and dried such that the thin green layer will be hardened due to the bonding effect of the inorganic binder. A portion of the thin green layer exposed under a directed high-energy beam is sintered, preferably by a laser beam, to cause ceramic molecules to bond together locally due to heat fusion. By controlling the scanning path of the high-energy beam, a two-dimensional thin cross section of the ceramic part in arbitrary form can be produced. A second thin ceramic layer can be built onto the first thin ceramic layer and bonded to it by the same method. After multiple repetitions of this procedure a three dimensional ceramic part can be fabricated layer upon layer. The green portion, which is not scanned by the high-energy beam, will be removed with suitable method. A ceramic part can be rapidly produced in this way.

Methods for fast generating spatially reduced-size images directly from compressed video streams supporting the coding of interlaced frames through transform coding and motion compensation. A sequence of reduced-size images are rapidly generated from compressed video streams by efficiently combining the steps of inverse transform, down-sampling and construction of each field image. The construction of reduced-size field images also allows the efficient motion compensation. The fast generation of reduced-size images is applicable to a variety of low-cost applications such as video browsing, video summary, fast thumbnail playback and video indexing.

The invention provides methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface. The substrates surfaces can be flat or geometrically altered to include wells of receiving material. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface. The tool can form an array of sample material by dispensing sample material in a series of steps, while moving the pin to different locations above the substrate surface to form the sample array. The invention then passes the prepared sample arrays to a plate assembly that disposes the sample arrays for analysis by mass spectrometry. To this end, a mass spectrometer is provided that generates a set of spectra signal which can be understood as indicative of the composition of the sample material under analysis.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

An automated method of rapidly producing customized 3D graphics images in which various user images and video are merged into 3D computer graphics scenes, producing hybrid images that appear to have been created by a computationally intensive 3D rendering process, but which in fact have been created by a much less computationally intensive series of 2D image operations. To do this, a 3D graphics computer model is rendered into a 3D graphics image using a customized renderer designed to automatically report on some of the renderer's intermediate rendering operations, and store this intermediate data in the form of metafilm. User images and video may then be automatically combined with the metafilm, producing a 3D rendered quality final image with orders of magnitude fewer computing operations. The process can be used to inexpensively introduce user content into sophisticated images and videos suitable for many internet, advertising, cell phone, and other applications.

A fireproof insulating foamed cementitious composition with thermal energy storage capacity is provided for use in producing wall insulation boards, fireproof claddings for steel structures, inner cores of fire resistant wall or door panels, and the like. The composition demonstrates improved energy efficiency in which phase change materials, such as microencapsulates, are used in conjunction with a cementitious mixture of calcined gypsum and hydraulic cement, lightweight aggregates, a polymer latex, and a foaming solution to create stable air bubbles inside the cementitious matrix. The calcined gypsum and the hydraulic cement are present in a weight ratio range from about 1:3 to about 3:1. The composition may further include reinforced fibers, surfactants, inorganic flame retardants, and other additives. The presence of the phase change material not only increases energy efficiency of the cured cementitious foam material, but also improves compatibility between calcined gypsum and cement during slurry mixing and hardening.

Oxazolidinones and methods for their synthesis are provided. Also provided are combinatorial libraries comprising oxazolidinones, and methods to prepare the libraries. Further provided are methods of making biologically active oxazolidinones as well as pharmaceutically acceptable compositions comprising the oxazolidinones. The methods of library preparation include the attachment of oxazolidinones to a solid support. The methods of compound preparation in one embodiment involve the reaction of an iminophosphorane with a carbonyl containing polymeric support.

A system and methods are provided for evaluating the position of surgical implants or openings formed in anatomical tissue during medical procedures, in which an initial 3-dimensional image of the anatomical tissue is combinable with one or more subsequent 3-dimensional images of the same or correlating tissue, and without the use of ionizing radiation on a patient for at least the subsequent images. The system includes a software program, a computer with display, a radiographic image scanning device, and a non-radiographic image scanning device. The computer accesses a medical patient information database and a medical implant database for images used by the software program. A pre-operative image is combined with subsequent images to facilitate evaluation of the proposed placement of a surgical implant or opening relative to tissues of the patient anatomy. Methods of evaluating the accuracy of surgical guides and of fabricating surgical guides are also disclosed.

An integrated process for the production of ethanol from woody plant material is provided, the process comprising: contacting a continuous flow of the plant material with a counter-current continuous flow of an aqueous ethanol solution at elevated temperature and pressure to provide plant material depleted of lignin; removing ethanol from the lignin-depleted plant material; contacting a continuous flow of the lignin-depleted plant material with water at elevated temperature and pressure to solubilize xylose within the plant material; and hydrolyzing cellulose present in the plant material to form glucose, which in turn is fermented to produce ethanol.

A method of aiding creation of a service offer associated with a Service Delivery Framework (SDF) includes providing a plurality of reusable Application Objects (AOs) that may be associated with an Interactive Development Environment (IDE). The AOs are prototype customer facing service offers that include standardized functions supporting ordering, billing, management and monitoring. The AOs also include standardized event formats and configurable attributes that affect the behavior and pricing of service offers derived from the AOs. A Services Marketplace facilitates reuse of AOs and supports relationships between customers, application creators, service providers and OSS / BSS providers. A computer-readable medium includes instructions that when executed by a computing device aids in creation of a service offer associated with a SDF by providing a plurality of reusable Application Objects (AOs) in the context of a services marketplace.

The invention provides compositions and methods for the detection and / or quantification of biological targets (e.g., nucleic acids and proteins) by the nucleic acid-templated creation of one or more reaction products, for example, epitopes, enzyme substrates, enzyme activators, and ligands. The reaction products can be detected and / or quantitated after signal amplification using an amplification system.

Disclosed is a method for producing colloidal graphene dispersions comprising the steps of(i) dispersing graphite oxide in a dispersion medium to form a colloidal graphene oxide or multi-graphene oxide dispersion(ii) thermally reducing the graphene oxide or multi-graphene oxide in dispersion.Dependent on the method used for the preparation of the starting dispersion a graphene or a multi-graphene dispersion is obtained that can be further processed to multi-graphene with larger inter-planar distances than graphite. Such dispersions and multi-graphenes are for example suitable materials in the manufacturing of rechargeable lithium ion batteries.

Processes for producing interbody spinal implants having a body with a top surface, a bottom surface, opposing lateral sides, opposing anterior and posterior portions, a substantially hollow center, and a single vertical aperture; and optionally, one or two integration plates affixed to the body. The processes include applying an additive process, a subtractive process, or both processes to at least one surface of the interbody spinal implant to form a roughened surface topography having a regular repeating pattern. The roughened surface topography is specifically designed to provide certain frictional characteristics, load dispersion, and to influence the biological responses that occur during bone healing and fusion.

This invention provides a system for performing PCR, and real time PCR in particular, with great speed and specificity. The system employs a heat block containing a liquid composition to rapidly transfer heat to and from reaction vessels. The system makes use of the reflective properties of the liquid metal to reflect signal from the PCR into the vessel and out the top. In this way, the signal can be measured by an optical assembly in real time without removing the vessels from the heat block.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of mouse embryonic fibroblast feeder cells to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by defined components added to the culture environment that support rapid proliferation without differentiation. The medium contains an isotonic buffer, a blend of essential nutrients such as protein and lipids, and an effective growth factor or combination of factors that promote proliferation while inhibiting differentiation. Culturing human embryonic stem cells in fresh medium on an extracellular matrix according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

An integrated process for the production of ethanol from woody plant material is provided, the process comprising: contacting a continuous flow of the plant material with a counter-current continuous flow of an aqueous ethanol solution at elevated temperature and pressure to provide plant material depleted of lignin; removing ethanol from the lignin-depleted plant material; contacting a continuous flow of the lignin-depleted plant material with water at elevated temperature and pressure to solubilize xylose within the plant material; and hydrolyzing cellulose present in the plant material to form glucose, which in turn is fermented to produce ethanol.

An automatic vending machine for fruit juice and a control method thereof. The vending machine includes a main body having both a fruit storage unit for storing fresh fruit in a refrigerated state and a fruit feeding unit for feeding stored fresh fruit in the main body; a rotary arm unit having two rotary arms that rotate in opposite directions such that the fresh fruit, fed by the fruit feeding unit, is seated and held by the two rotary arms; a fruit cutting unit installed below the rotary arm unit and having a blade for cutting the fruit, held by the two rotary arms, into two pieces when the fruit is moved downwards; a fruit crushing unit installed below the fruit cutting unit and having two crushing bars, which are placed vertically and crush the fruit pieces held by the two rotary arms to make fruit juice when the two rotary arms holding the respective fruit pieces are rotated downwards and move downwards; and a control unit for recognizing an input bill or an input coin and controlling the vending machine in accordance with manipulation of a user.

The invention discloses a buffering and energy absorbing structure. The buffering and energy absorbing structure comprises a housing. The buffering and energy absorbing structure is a multi-layer metal grid structure or porous bubble structure filled in the housing; the metal grid structures or porous bubble structures of adjacent layers are lengthways alternatively overlapped in positive and negative poisson ratio; the metal grid structure in the positive poisson ratio is composed of a three-dimensional honeycomb grid structured formed by array type regular hexagonal pore grids; the metal grid structure in the negative poisson ratio is composed of a three-dimensional stretching and expanding grid structure formed by array type concave angle pore grids; the meshes of the porous bubble structure in the positive poisson ratio are of an array type rhombus structure; the meshes of the porous bubble structure in the negative rhombus structure are of an array type four-star shaped structure. Compared with the traditional buffering and energy absorbing structure, the buffering and energy absorbing structure can effectively reduce the peak value of the impact force, so that the whole energy absorbing process is stable, and as a result, the energy absorbing efficiency of the structure can be increased.

A polypeptide self-antigen useful in a tumor-specific vaccine mimics one or more epitopes of an antigen uniquely expressed by cells of the tumor. The polypeptide is preferably produced in a plant that has been transformed or transfected with nucleic acid encoding the polypeptide and is obtainable from the plant in correctly folded, preferably soluble form without a need for denaturation and renaturation. This plant-produced polypeptide is immunogenic without a need for exogenous adjuvants or other immunostimulatory materials. The polypeptide is preferably an scFv molecule that bears the idiotype of the surface immunoglobulin of a non-Hodgkin's (or B cell) lymphoma. Upon administration to a subject with lymphoma, the plant-produced, tumor-unique scFv polypeptide induces an idiotype-specific antibody or cell-mediated immune response against the lymphoma.

A process for producing metal oxide thin films on a substrate by the ALD method comprises the steps of bonding no more than about a molecular monolayer of a gaseous metal compound to a growth substrate, and converting the bonded metal compound to metal oxide. The bonded metal compound is converted to metal oxide by contacting it with a reactive vapour source of oxygen other than water, and the substrate is kept at a temperature of less than 190° C. during the growth process. By means of the invention it is possible to produce films of good quality at low temperatures. The dielectric thin films having a dense structure can be used for passivating surfaces that do not endure high temperatures. Such surfaces include, for example, organic films in integrated circuits and polymer films such as in organic electroluminescent displays and organic solar cells. Further, when a water-free oxygen source is used, surfaces that are sensitive to water can be passivated.

A method of preparing a multimedia recording of a live presentation, where the presentation comprising a plurality of events, is provided. The method includes the steps of, (a) preparing a recording means and gathering information associated with said presentation, (b) recording the events of said presentation, (c) digitizing any event recording not digitally recorded, (d) transferring said recordings to said electronic storage medium, (e) processing said recordings to create a digital multimedia presentation wherein said events are presented in a visual and audio format and the staging of said visual and audio formats are automatically synchronized, and displaying said digital multimedia presentation.

A device and method for reinforcing a blister, including a transport mechanism for transporting the blister, and a centering mechanism that bring the blister into a particular position in the direction transverse to the travel direction of the transport mechanism. A gluing station applies an adhesive to the blister and / or to a card, with the card having at least one opening that is matched to the geometry of a pocket of the blister. An insertion device is provided that sets the card into place on the blister.

The present invention provides a healing abutment for attachment to a dental implant with marking locations thereon. The marking locations either lack or have markers that provide a binary code system for retrieving unique information about the healing abutment and the underlying implant.

A method for starting a fast light-off catalytic reformer for producing hydrogen-rich reformate fuel from hydrocarbon fuel and air, the reformer having means for receiving flows of fuel and air, a reforming catalyst for reforming the fuel and air mixture, and an ignition device. A control system selects fuel and air flow rates to form a lean fuel / air mixture and operates the ignition device to ignite the lean mixture to produce hot exhaust gases that flow over and heat the reforming catalyst for a first length of time. Fuel flow is then stopped temporarily for a second length of time, and further ignition is terminated. Fuel flow is then restarted and adjusted to provide a rich fuel / air mixture which is directed to the heated catalyst for reforming into reformate fuel. Air flow may also be adjusted in setting the lean and / or rich fuel / air mixtures.

A method for creating and a device for a rotary switchable multi-core element, permanent magnet-based apparatus, for holding or lifting a target, comprised of two or more carrier platters, each containing a plurality of complementary first and second core elements. Each core element comprises permanent magnet(s) with magnetically matched soft steel pole conduits attached to the north and south poles of the magnet(s). Core elements are oriented within adjacent carrier platters such that relative rotation allows for alignment in-phase or out-of-phase of the magnetic north and south fields within the pole conduits. Aligning a first core element “in-phase” with a second core element, that is, north-north / south-south, activates that core element pair, allowing the combined magnetic fields of the pole conduits to be directed into a target. Aligning the core element pair “out-of-phase,” that is, north-south / south-north, deactivates that core element pair by containing opposing fields within the pole conduits.

Lentiviral vectors modified at the 5′ LTR or both the 5′ and 3′ LTR are useful in the production of recombinant lentivirus vectors (See the Figure). Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production. The vectors can contain inducible or conditional promoters.