The invention discloses a micro-fluidic
chip structure for a flow cytometer, and a preparation method a micro-fluidic
chip. The
chip comprises a sample liquid inlet 1, a column array structure 6, a conical focusing structure 7, a micro-channel 8, a detection zone 4, a flow expanding channel 9 and a waste liquid outlet end 5, wherein the column array structure 6 can play a role of filtering
agglomerate protein impurities and other large biological solids inside sample liquid; and the conical focusing structure 7 has a focusing effect which is similar to that of the traditional sheath
liquid system, so that
cell granules flow inside the micro-channel 8 one by one; and the micro-channel 8 restricts the cells through the channel, so that the cells pass through the detection zone 4 one by one. By adopting the chip structure disclosed by the invention, the focusing effect without sheath flow liquid can be achieved; and blockage is not generated, so as to reduce use of the sheath flow liquid; and meanwhile, the situation that the
cell granules pass through the detection zone one by one is ensured. The
processing method of the micro-fluidic chip is convenient; the used bonding method is a common bonding method, and is simple and convenient to operate; and the used material PDMS (
polydimethylsiloxane) is easy to process and has good
biocompatibility.