148results about How to "Inhibit transfer" patented technology

The invention discloses a double-layer microneedle patch and a preparation method thereof.The preparation method of the double-layer microneedle patch comprises the following steps that firstly, 3D printing is conducted through polydimethylsiloxane, and a PDMS mold similar to a machining microneedle is obtained; dispersing the medicine into the sericin solution to serve as an outer layer solution; dispersing germanium nanoparticles into the polycaprolactone solution to obtain an inner layer solution; and finally, adding the outer-layer solution into the needle-shaped groove of the PDMS mold, so that the needle-shaped groove is filled with the outer-layer solution, drying, adding the inner-layer solution, drying, and demolding to obtain the double-layer microneedle patch. When the double-layer microneedle patch is used for drug delivery, drugs can be dissolved and released in a short time, the inner layer is polycaprolactone (PCL) carrying photo-thermal agent germanium nanoparticles, degradation is slow, a photo-thermal effect is generated under irradiation of 808nm near-infrared light, residual tumor cells are induced to die immunogenicity, an immune microenvironment is programmed in a time sequence, and the double-layer microneedle patch has the advantages of being high in sensitivity, high in sensitivity and good in stability. Postoperative recurrence and metastasis of tumors are efficiently inhibited, and meanwhile operative wound repair is promoted.

The invention provides an ultrahigh-ductility cement-based composite material for ocean engineering and a preparation method of the ultrahigh-ductility cement-based composite material for ocean engineering. The concrete is prepared from the following raw materials in parts by weight: 250 to 500 parts of cement clinker, 400 to 600 parts of fly ash, 200 to 300 parts of mineral powder, 100 to 150 parts of silica fume, 160 to 240 parts of metakaolin, 600 to 900 parts of quartz sand, 10 to 20 parts of organic synthetic fiber, 20 to 30 parts of organic corrosion inhibitor, 180 to 240 parts of water and 1 to 8 parts of water reducing agent. The organic corrosion inhibitor provided by the invention can be combined with chloride ions to form a passive film, so that the corrosion of reinforcing steel bars and the invasion of the chloride ions into concrete are inhibited, and the durability of the concrete is improved; by regulating and controlling the mixing ratio of the four mineral admixtures and the mixing amount of the synthetic fiber component, the concrete can well consider high strength, high ductility, high durability, high flow state and excellent deformation resistance. The ultrahigh-ductility concrete for ocean engineering, which can meet the toughness requirements of high compression resistance, fracture resistance and impact resistance and the requirements of high chloride ion permeation resistance and steel bar corrosion resistance, can be obtained.

A film for manufacturing a flexographic printing plate, which comprises a base material and an ablation layer laminated on the base material, the ablation layer containing a resin having a structural unit (1) represented by general formula (1), and the thickness of the base material being 10 [mu] m or more and less than 100 [mu] m. (In formula (1), R1 and R2 each independently represent a non-polar group, and R3 and R4 each independently represent a hydrogen atom or a non-polar group. > # imgabs0 #

The invention relates to bionic ferroptosis nanoparticles loaded with superparamagnetic iron oxide nanoparticles as well as a preparation method and application of the bionic ferroptosis nanoparticles. The biomimetic ferroptosis nano-particles are obtained by loading the superparamagnetic iron oxide nano-particles packaged by the biomimetic platelet membrane, Erastin and lactate oxidase together, and are used for guiding magnetic resonance imaging and inducing tumor cell ferroptosis. According to the method, ferroptosis is induced through Erastin, lactic acid is removed through lactate oxidase, and ferroptosis is enhanced. In addition, lactic acid is oxidized to generate hydrogen peroxide, the hydrogen peroxide is converted into cytotoxic hydroxyl radicals, and cell death is further accelerated. When the PM-ESLNP is combined with an alphaPD-L1 immunotherapy, the PM-ESLNP obviously inhibits tumor growth and metastasis in a liver cancer mouse model, and the result exceeds that of a single immunotherapy. In addition, the superparamagnetic nanoparticles in the PM-coated ESLNP can be used for carrying out real-time MRI (Magnetic Resonance Imaging) monitoring on the treatment effect. According to the invention, a groundbreaking MRI guiding method is introduced, so that ferroptosis of tumors can be enhanced and tumor immunotherapy can be synergistically improved.

The invention relates to an application of galuteolin-sensitized cis-platinum in preparation of a medicine for treating esophageal squamous cell carcinoma. It is found that the galuteolin can block the progress of esophageal squamous carcinoma cells in multiple links including apoptosis induction, growth and invasion inhibition and the like, the galuteolin can also significantly enhance inhibition of cis-platinum on lymphatic metastasis of esophageal squamous carcinoma cells in a mouse model, and the galuteolin and the cis-platinum have a significant synergistic effect.

The invention discloses application of PABPC3 as a marker for evaluating prognosis of metastatic ovarian cancer, and belongs to the technical field of biological diagnosis and medicine. Specifically, differential genes in an in-situ ovarian cancer and a metastatic ovarian cancer in a database are screened, and are combined with differential genes related to ovarian cancer prognosis in a pan-cancer database, so that a gene PABPC3 with remarkable differential expression is obtained. The influence of the gene PABPC3 on the activity, migration ability and the like of ovarian cancer cells is researched through cell experiments and in-vivo experiments, and the feasibility of the marker in evaluation of ovarian cancer prognosis is proved. Meanwhile, the invention also discovers that the expression of the knock-down gene PABPC3 can inhibit the metastasis of cancer cells. Therefore, the invention provides a novel strategy for detecting and treating ovarian cancer metastasis, which is expected to overcome the defects of the traditional detection and treatment mode, and provides a new choice for clinic.

The invention discloses a nano-carrier based on charge crosslinking, a preparation method of the nano-carrier and application of the nano-carrier as a tumor chemotherapy drug. Wherein the nano-carrier is formed by assembling two polymer chains with positive and negative charges through electrostatic interaction, a precursor ligand on a polymer chain B releases an organic ligand under exogenous or endogenous stimulation, and competes for metal ions on a polymer chain A with positive charges to generate a metal complex in situ, and meanwhile, the electrostatic interaction of the nano-carrier is weakened, so that the metal complex is formed. The dissociation of the nano-carrier is caused, a macromolecular chain B containing an organic ligand is released, and the effect of tumor chemotherapy or immunoregulation is achieved. The method is simple to operate, short in time consumption and mild in reaction condition; the material is good in biocompatibility and high in safety degree. Pharmacodynamic experiments in some embodiments show that the nano-carrier can effectively kill the breast cancer and trigger cellular immunogenic death, meanwhile, tumor cell metastasis is inhibited, and a new thought and means are provided for the clinical problem of breast cancer lung metastasis urgently needing to be solved at present.

The invention belongs to the field of tumor drugs, and discloses a radioactive hydrogel based on crosslinking of sodium alginate and metal ions and a preparation method and application thereof.The preparation method of the radioactive hydrogel comprises the following steps that lutetium chloride and metal ions are mixed, and a metal ion mixed solution is obtained; doping 177Lu radioactive isotope into the obtained metal ion mixed solution to obtain a metal ion cross-linking agent; and injecting a metal ion cross-linking agent into the sodium alginate solution under a vortex condition, centrifuging, and discarding the supernatant to obtain the radioactive hydrogel. The radioactive hydrogel (177Lu-RH) provided by the invention has good in-tumor colonization capability, can be directly injected into a tumor to realize local radiotherapy, has good radioactive stability, and can be stably retained at a tumor part for more than 120 hours, so that the stability of radioactive labeling in a treatment process is ensured.

The invention relates to the field of soil remediation, in particular to a method for preventing and controlling transfer of cadmium in hyperaccumulators, which comprises the following steps: planting annual ryegrass in cadmium-containing soil, and applying a leaf surface preventing and controlling agent to the annual ryegrass. According to the method, the leaf surface resistance and control agent is added into the annual ryegrass, the enrichment capacity of the underground part of the plant to cadmium is enhanced through the leaf surface resistance and control agent, transfer of cadmium of the underground part of the plant to the overground part of the plant is reduced, a large amount of biomass polluted by cadmium is prevented from being generated, and the cadmium of the overground part of the plant meets the regulation of pollutant limit in food in the national food safety standard.

The invention relates to a universal hydrogel vaccine based on tumor neoantigen peptide as well as a preparation method and application of the universal hydrogel vaccine. The hydrogel vaccine comprises tumor neoantigen peptide hydrogel and an adjuvant loaded on the tumor neoantigen peptide hydrogel, the tumor neoantigen peptide-peptidyl assembly sequence conjugate is assembled in a solvent to form a nanofiber solution, and the nanofiber solution is triggered to form hydrogel under physiological conditions; the conjugate is obtained by coupling a tumor neoantigen peptide and a peptidyl assembly sequence through a covalent bond; the adjuvant is at least any one of a cell factor, an STING agonist, a Toll-like receptor agonist and an immune cell. The vaccine disclosed by the invention has universality, aiming at different tumor neoantigen peptides, self-assembly of a conjugate in a solvent is realized by regulating and controlling the quantity of valine or phenylalanine in a peptidyl assembly sequence to form a nanofiber solution, and the vaccine adjuvant is loaded through physical action to obtain a nanofiber solution containing the vaccine adjuvant; therefore, personalized cancer immunotherapy is realized.

The invention provides application of combination of tageteoside and difacitinib or ponatinib to blocking of esophageal squamous cell carcinoma progression. The tageteoside is combined with an FAK inhibitor defacitinib or an Src family kinase inhibitor ponatinib, so that the metastasis of esophageal squamous carcinoma, especially lymphatic metastasis, can be inhibited, the growth of primary focus and metastatic focus esophageal squamous carcinoma cells can be inhibited, and a synergistic anti-cancer effect is achieved.

The invention relates to the technical field of health-care products, in particular to a health-care product rich in momordica charantia polypeptide and capable of regulating blood sugar metabolism and promoting insulin secretion, momordica charantia polypeptide can stimulate pancreatic beta cells to secrete insulin, and insulin is the only hormone capable of reducing blood sugar in a human body. The balsam pear polypeptide component in the balsam pear extract is specially used for reducing the blood sugar level and improving related health problems, the application of the balsam pear polypeptide in the health care product can effectively regulate the blood sugar metabolism of a human body and promote the secretion of insulin, and the balsam pear polypeptide stimulates pancreas islet beta cells to promote the secretion of insulin. In addition, the balsam pear polypeptide can improve the insulin resistance, increase the number of insulin receptors or enhance the activity of the insulin receptors, so that the body cells are more sensitive to the insulin, the uptake and utilization of glucose are promoted, and the abnormal increase of the blood glucose level is avoided.

The present invention relates to a lithium secondary battery with improved lifetime characteristics, a driving method thereof, a battery module including the same, and a battery pack including the battery module, provides the lithium secondary battery having an operating voltage that is less than a plateau voltage of the lithium / nickel composite oxide, and thus has an advantage of being enable to prevent the lithium / nickel composite oxide from being transferred to a spinel-like structure, suppress the capacity degradation according to an increase in cycle, and improve a lifetime characteristic.

The present invention relates to the field of biomedicine, and specifically to a polypeptide that blocks HGF-HGFR binding and its application. The amino acid sequence of the polypeptide contains any one of a first amino acid sequence as shown in SEQ ID NO: 1 and a second amino acid sequence composed of a plurality of consecutive amino acids in the first amino acid sequence. The polypeptide provided by the present invention can effectively block the binding of HGF-HGFR, prevent HGFR from being overactivated and causing excessive cell proliferation to form tumors, cause tumor cell apoptosis, and inhibit tumor cell proliferation and tumor cell metastasis, thereby preventing and / or treating diseases related to its signaling pathway.

The application provides a preparation method of a diphenylfluorene polyether. The method uses diphenylfluorene as a substrate, butylene oxide as an epoxide monomer, toluene as an organic solvent, and under the action of a composite catalyst, reacts at 110-200 DEG C for 2-6 hours to obtain the diphenylfluorene polyether. The composite catalyst is obtained by reacting a porous aromatic skeleton with a bromoalkyl quaternary ammonium salt to obtain a quaternary ammonium salt functionalized porous aromatic skeleton, and finally, a basic catalyst is limitedly encapsulated in the pore channel of the quaternary ammonium salt functionalized porous aromatic skeleton by electrostatic attraction. The catalyst of the method can be recycled and reused, and the yield retention rate is greater than or equal to 92% after the composite catalyst is used for 5 cycles, and the method is suitable for optical display, high-frequency circuit board, semiconductor packaging and other fields.

The invention discloses nano-particles containing biological enzyme as well as a preparation method and application of the nano-particles. The double-drug nano-particle provided by the invention is prepared by taking a nano composite material Cu / ZIF-8 obtained by modifying a ZIF-8 carrier with a copper base as a carrier, wrapping PPE in the carrier, adsorbing a small molecule drug CB-839 outside to form a nano-drug, and modifying the nano-drug with hyaluronic acid. The double-drug nanoparticle improves the properties of free PPE and CB-839, improves the stability, prolongs the tumor residence time and enhances the curative effect; and PPE and CB-839 can be released under low pH response of lysosome in cells, so that high-efficiency and low-toxicity treatment of tumors is realized. The double-drug nanoparticle can achieve a synergistic anti-tumor effect that 1 + 1 is greater than 2, and has a good application prospect in preparation of anti-tumor drugs.

The utility model provides a medical waste cleaning device, which relates to the field of medical waste cleaning, and comprises a treatment box, a feeding assembly is arranged on the treatment box, the feeding assembly comprises an overturning plate, the overturning plate is connected onto the treatment box, a sliding rail is connected onto the overturning plate, a third rotating shaft is connected onto the sliding rail, a connecting rod is connected onto the third rotating shaft, and the connecting rod is connected onto the third rotating shaft. A second rotating shaft is connected to the connecting rod, a sliding frame is connected to the second rotating shaft, the sliding frame is connected to the sliding rail, clamping blocks are connected to the second rotating shaft, the number of the clamping blocks is two, an extending block is connected between the two clamping blocks in a clamped mode, and when the connecting rod moves, the second rotating shaft can slide on the sliding frame to drive the clamping blocks to slide out of sliding grooves in the sliding rail; and an electric telescopic rod drives an overturning plate to be lifted up, the waste box is driven to pour medical waste in the waste box into a material collecting barrel, and workers do not need to manually transfer the waste into a cleaning device.

The present invention pertains to the pharmaceutical field, and particularly relates to a pharmaceutical combination comprising a Bcl-2 inhibitor or a Bcl-2 / Bcl-xL inhibitor and one or more anticancer reagents, and the use of the combination to treat a disease such as cancer, espefically acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL) or multiple myeloma (MM) resistant or insensitive to Bcl-2 inhibitors and / or carrying Bcl-2 mutation and / or TP53 mutation. The invention also relates to a pharmaceutical composition or kit comprising the combination.

The present disclosure relates to a pharmaceutical composition for preventing or treating triple-negative breast cancer, comprising an oligonucleotide as an active ingredient.

The utility model belongs to the field of tree juice extraction devices, and discloses a handheld automatic juice extraction device which comprises an abutting plate and an extraction needle, the abutting plate is connected with a mounting frame, the mounting frame is rotationally connected with a first threaded rod and a second threaded rod, the mounting frame is connected with a servo motor, and an output shaft of the servo motor is connected with the first threaded rod. The extraction needle is connected with a fixing plate, the fixing plate is in threaded connection with the first threaded rod, the mounting frame is connected with an auxiliary sliding rod, the fixing plate is in sliding connection with the auxiliary sliding rod, the second threaded rod is in threaded connection with a positioning plate, the positioning plate is in sliding connection with the auxiliary sliding rod, the mounting frame is connected with scale marks, the extraction needle is provided with a liquid inlet hole, and the mounting frame is provided with a moving groove. The extraction needle is connected with a suction pipe, and the abutting plate and the mounting frame are provided with through holes respectively. According to the technical scheme, the insertion depth of the extraction needle head can be controlled, the situation that the extraction needle is inserted too deep or too shallow is avoided, liquid extraction efficiency and quality are improved, tree safety is protected, the structure is simple, and practicability is greatly improved.

The invention belongs to the technical field of medicines, and particularly relates to application of NOTCH3 serving as a target spot to preparation of a pharmaceutical composition for treating bladder cancer. The invention relates to a targeted inhibitor of NOTCH3, which is shRNA (short hairpin ribonucleic acid). Experimental results show that NOTCH3 is a key oncogene of bladder cancer and can be used as a therapeutic target of bladder cancer, and an inhibitor of NOTCH3 can be used for preparing a pharmaceutical composition for treating bladder cancer.

This invention discloses the application of a branched-chain amino acid (BCAA) restricted diet in the preparation of drugs for treating liver metastases of tumors. Through mouse model experiments, this invention found that restricting the intake of BCAAs (valine, isoleucine, and leucine) in the diet, or using the SCD1 inhibitor A939572, can significantly inhibit liver metastases of tumors, especially pancreatic cancer liver metastases. Experimental results show that a BCAA restricted diet or SCD1 inhibitor can reduce the number and volume of liver metastases and inhibit their growth. This invention also reveals its mechanism of action: BCAA restriction reduces the propionylation modification of SREBP1, downregulates the transcriptional expression of its target gene SCD1, and thus reduces lipid synthesis, thereby exerting an anti-tumor liver metastasis effect. This invention provides a novel dietary intervention strategy and drug target for the treatment of liver metastases of tumors.

The application discloses a method for preparing polyethylene wax by using a carborane bridged rare earth metallocene catalyst, and simultaneously preparing a carborane bridged indenyl rare earth metallocene compound as a main catalyst, loading the prepared main catalyst and a cocatalyst on a magnetic mesoporous Fe3O4@Al2O3 core-shell nanoparticle carrier to obtain a supported rare earth metallocene catalyst; the main catalyst of the application is a carborane bridged indenyl rare earth metallocene compound with a structure shown in formula (I), wherein a bridging group adopts a closed carborane (closed-1,2-dicarbadodecaborane), and the cage-like three-dimensional structure has super-high thermal stability and chemical inertness and does not degrade or rearrange in a high-temperature molten state of 130-200 DEG C. Large-volume substituents such as methyl, tert-butyl, phenyl or 4-tert-butylphenyl are introduced on the indene ring to provide sufficient steric protection and further inhibit chain transfer and side reactions at high temperatures.

The present invention discloses an immune-enhancing targeted hollow manganese dioxide radiotherapy sensitizer and its preparation method and application, which belongs to the field of tumor treatment technology. Aiming at the problems of short circulation time and poor targeting of radiotherapy sensitizers, the present invention adopts an engineered extracellular vesicle modification method to improve the circulation time, so that it has tumor targeting; Aiming at the problem of poor improvement effect of hypoxic microenvironment in the prior art, the present invention prepares hollow structure MnO2 by template self-etching method to improve the catalytic efficiency of overexpressed hydrogen peroxide in tumor cells, and uses metformin, a cell respiration inhibitory drug, in synergy to reduce cell oxygen consumption, improve hypoxic microenvironment from two aspects, and enhance radiotherapy sensitization effect; At the same time, the present invention effectively regulates the immunosuppressive microenvironment of tumors by reducing manganese ions generated by sensitizers, and activates the cGAS-STING pathway to enhance the activity of natural immune cells, release immune factors, and inhibit the metastasis and recurrence of tumors after radiotherapy.

The present invention discloses a lymph node targeted tumor in situ vaccine and a preparation method and application thereof, which belongs to the field of biomedicine technology. The immune adjuvant CpG is coated on mannose nanoparticles to obtain a CpG nanoadjuvant, and then an MMP responsive gel is used as a delivery system to deliver the CpG nanoadjuvant and trametinib together, thereby achieving sustained in situ antigen production, lymph node drainage and APCs presentation, and further activating anti-tumor immune response. The vaccine has a use in preparing vaccine drugs for treating tumors, and as an effective cancer vaccine for immunotherapy, achieves targeting to lymph nodes to induce anti-tumor immunity.

The invention belongs to the technical field of medical preparations, and particularly relates to a resveratrol-succinyl glycan-tetravalent platinum nano-drug as well as a preparation method and application of the resveratrol-succinyl glycan-tetravalent platinum nano-drug. In the resveratrol-succinyl glycan-tetravalent platinum nano-drug provided by the invention, the succinyl glycan (RIC) can exert the anti-tumor activity by improving the immune function of a body; resveratrol (RES) can be used for directly acting on related targets and the like to exert anti-tumor activity by inhibiting proliferation of tumor cells, inducing apoptosis of the tumor cells, inhibiting invasion and metastasis of the tumor cells and regulating the immune function of a body. According to the invention, the succinylglycan-tetravalent platinum conjugate is used for wrapping an anti-cancer drug micromolecule RES, so that the anti-cancer curative effect of the drug is improved, the problems of toxic and side effects of platinum drugs, drug resistance of tumor cells and the like are solved, and a new idea is provided for construction and application of platinum nano-drugs.

The invention discloses a chimeric antigen receptor targeting human alpha-enolase, a CAR-T cell and application of the chimeric antigen receptor. The invention relates to a chimeric antigen receptor targeting human alpha-enolase, the chimeric antigen receptor comprises an scFv structural domain aiming at the human alpha-enolase, a transmembrane region and an intracellular signal transduction region, and the amino acid sequence of the chimeric antigen receptor is SEQUENCE No.1. The chimeric antigen receptor aiming at the tumor metabolic protein ENO1 is constructed, on one hand, tumor cells are recognized and killed through the highly expressed ENO1, and on the other hand, the chimeric antigen receptor has the effect of inhibiting invasion and metastasis of the tumor cells. Compared with ENO1 monoclonal antibody targeted therapy, the ENO1CAR therapy can directly kill tumor cells, and the effect is stronger; compared with other CAR treatment targets, the ENO1 has more adaptations and can play a role in inhibiting invasion and metastasis of tumors.

The present invention discloses an application of a genetically engineered bacterium VNP20009-M of attenuated Salmonella typhimurium in the preparation of a drug for preventing and treating post-metastatic cancer. The genetically engineered bacterium VNP20009-M has tumor targeting and significant effects of inhibiting metastasis and growth on cancer cells, and can be used for the preparation of a drug for preventing and treating post-metastatic tumors.

The invention discloses a gallium-indium alloy nano dispersion system, preparation thereof and application of the gallium-indium alloy nano dispersion system in radio frequency sensibilization, and belongs to the cross technical field of chemistry, medicine and other subjects. Raw materials of the gallium-indium alloy nanometer dispersion system comprise gold nanoparticles prepared by a glutathione reduction method and nanometer liquid drops obtained after ultrasonic treatment of liquid metal gallium-indium alloy, and electrochemical replacement occurs in the process that the gold nanoparticles prepared by the glutathione reduction method make contact with the liquid metal nanometer liquid drops. And adsorbing on the surface of the nano liquid drop of the liquid metal gallium-indium alloy after ultrasonic treatment. The gallium-indium alloy nano dispersion system can generate various reactive oxygen free radicals under the action of radio-frequency electromagnetic waves, enables the temperature of an irradiated part to rise quickly, cooperatively kills tumor cells, activates whole-body anti-tumor immune response, and effectively inhibits tumor recurrence and metastasis. The gold nanoparticles not only can significantly improve the stability of the nano-dispersion, but also endow the nano-dispersion with catalase activity, and further enhance the antitumor activity of the nano-dispersion.

The application provides a polypeptide for specifically blocking the combination of ACVR1C and Grem1 and application thereof, and belongs to the technical field of biological medicine. The application finds a new combination of receptors of Grem1 and ACVR1C, and determines the combination sites of the two; then according to the details of the combination of Grem1 and ACVR1C, a specific blocking small molecule polypeptide (ACVR1C peptide) is designed. The small molecule polypeptide can interfere with or block the combination of Grem1 and ACVR1C in an in-vitro experiment; in an in-vivo treatment experiment, the liver metastasis of colon cancer of the treatment group mice is obviously lower than that of the control group, which indicates that the small molecule polypeptide reaches the target of blocking the liver metastasis of colon cancer by interfering with or blocking the combination of Grem1 and ACVR1C, and provides a new drug design and treatment target for relieving the liver metastasis of colon cancer.