152 results about "Large cell" patented technology

A rare-earth-containing high-silicon Y-type zeolite, the zeolite contains rare earths, is characterized in that the silicon-aluminum ratio of the zeolite is 5-30, the initial unit cell constant is 2.430-2.465 nanometers, and the balance unit cell constant and the initial unit cell constant The ratio is at least 0.985. The zeolite has the characteristics of small initial unit cell constant and large equilibrium unit cell constant. It has high structural stability and hydrothermal stability. The cracking catalyst containing this zeolite is not easy to deactivate. The choice of gasoline, diesel oil, dry gas and coke Good sex.

A cell-specific dictionary is applied adaptively to adequate cells, where the cell-specific dictionary subsequently optimizes the handling of frequency-partitioned multi-dimensional data. This includes improved data partitioning with super cells or adjusting resulting cells by sub-dividing very large cells and merging multiple small cells, both of which avoid the highly skewed data distribution in cells and improve the query processing. In addition, more efficient encoding is taught within a cell in case the distinct values that actually appear in that cell are much smaller than the size of the column dictionary.

An expandable stent has larger cells located at the distal end of the stent than in the body portion so that a catheter balloon can more easily protrude into the cells to increase stent retention relative to the balloon. The intravascular stent has a plurality of cylindrical rings connected by links, the spacing of which is a factor in defining the cell size. The stent can be compressed or crimped onto a balloon catheter to a very low profile and maintain a high degree of stent retention due to increased spacing between rings in the region of the distal end ring.

Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates.

A method and apparatus is provided for transmitting an orthogonal frequency domain multiple access (OFDMA) signal including a synchronization channel signal transmitted within a localized portion of a bandwidth of the OFDMA signal (818), the synchronization channel signal having predetermined time domain symmetry within the localized portion of the bandwidth (816) and including information for providing at least partial cell identification information (812). The synchronization channel signal enables an initial acquisition and cell search method with low computational load which provides OFDMA symbol timing detection and frequency error detection (1112) and frame boundary detection and cell specific information detection (1114) in an OFDMA system supporting multiple system bandwidths, both synchronized and un-synchronized systems, a large cell index and an OFDMA symbol structure with both short and long cyclic prefix length.

The invention provides a multi-parameter paper-chip electrochemical immunosensor which comprises a covering layer, a filtering film and a foldable paperchip, wherein the covering layer is provided with a sample introduction hole, and first comb teeth which are used for exposing the end of a metal lead wire on the foldable paperchip; the filtering film is closely attached to a position below the sample introduction hole to filter and intercept large cells in a sample; the foldable paperchip comprises a first filter paperchip, a second filter paperchip and a broken line located in a middle position, sensitive areas and central drainage holes which are formed in the first filter paper chip, and working electrodes and an Ag-AgCl electrodes which are arranged on the second filter paper chip along the broken line, are symmetrically distributed; craphene-metal composite nano-materials, electronicmediators and various antibodies are decorated in the sensitive areas. After specific recognition with various substances to be detected, the filter paper chip is folded to be used for detecting the electrochemical signal change caused by the substances to be detected in each sensitive area. The invention also provides a method for detecting multiple lung cancer markers.

Compounds of the present invention find utility in the treatment of mammalian cancers and especially human cancers including, but not limited to, malignant melanomas, solid tumors, glioblastomas, ovarian cancer, pancreatic cancer, prostate cancer, lung cancers, breast cancers, kidney cancers, hepatic cancers, cervical carcinomas, metastasis of primary tumor sites, myeloproliferative diseases, chronic myelogenous leukemia, leukemias, papillary thyroid carcinoma, non-small cell lung cancer, mesothelioma, hypereosinophilic syndrome, gastrointestinal stromal tumors, colonic cancers, ocular diseases characterized by hyperproliferation leading to blindness including various retinopathies, diabetic retinopathy, rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, mastocytosis, mast cell leukemia, and diseases caused by PDGFR-α kinase, PDGFR-β kinase, c-KIT kinase, cFMS kinase, c-MET kinase, and oncogenic forms, aberrant fusion proteins and polymorphs of any of the foregoing kinases.

An improved centrifuge system having a single means for both feeding and collecting liquid streams aseptically from rotating components is provided. Also methods and apparatus for centrifugal separation of cells from cell culture media of large cell culture batches by processing a large volume within a few hours, using pre-sterilized, single-use fluid path components. The apparatus uses a sealing approach that improves reliability while avoiding air contamination as well as shedding from mechanical seals. The risk of process liquid leaks is minimized.

Disclosed is a hierarchical random access method for a wireless communication system having a significantly large cell. According to the present invention, a length of a preamble sequence and a length of a reference slot may be designed based on a terminal having greatest capacity of adjusting a timing error arrived at a base station, and a slot length may be designed to be an integer multiple of the length of the reference slot depending on a timing error correction capacity, thereby enabling terminals to use various slot lengths.

The invention provides an acquisition method for exosomes derived from human urinary cells. The acquisition method includes the steps that firstly, human urine source cells are separately cultured, a culture medium is collected, the culture medium of the human urine source cells is filtered through a 0.22-micrometer filter membrane, and then large cell fragments and other impurities are removed; then an organelle is centrifugally removed, and supernatant is reserved; a membrane capable of intercepting 100KD molecular weight is used, the exosomes in the supernatant are centrifugally intercepted, after interception, PBS is used for eluting the membrane, and an exosome concentrated solution is obtained. The exosomes can be used for preparing medicine with the effects of resisting apoptosis, promoting angiogenesis, restoring ischemia damage and promoting cell growth and used for treating skin defects, skin ulcers, pressure sores, bone defects, bone ununion, femoral head necrosis, renal injury, ischemic injury, spinal cord injury, islet damage, diabetes, complications of diabetes, Alzheimer's diseases and the like.

The invention discloses a biologically-safe quality control material for a blood tester and a preparation method thereof. The preparation method comprises that animal red blood cells having the sizes similar to sizes of human red blood cells are used for simulation of human red blood cells; animal red blood cells having the sizes similar to sizes of large cells in human white blood cells and animal red blood cells have the sizes similar to sizes of small cells in human white blood cells are used for simulation of human white blood cells; animal red blood cells have the sizes similar to sizes of human blood platelets are used for simulation of human blood platelets; the animal red blood cells for simulation of human white blood cells and human blood platelets are cured by formaldehyde having the content of 2-8%; the cured animal red blood cells are cleaned and then are added with bovine serum albumin so that the cure agent is removed; and according to requirements, through blending, the single-component or whole blood quality control material is obtained. The biologically-safe quality control material has good stability, a low cost and high safety, and is suitable for a blood cell analyzer, a urinary sediment analyzer and a hemoglobin analyzer.

In a liquid crystal display device, a thickness of a liquid crystal layer in each pixel is adjusted based on a display color (red, green, and blue). For example, thicknesses of color filters corresponding to the display colors are varied based on the display color to set the thickness (cell gap) of the liquid crystal layer in each pixel to a predetermined value corresponding to a transmittance of the liquid crystal. A spacer provided to maintain the cell gap at a constant value is not provided in a region, among the plurality of display colors, having a large cell gap and the spacer is provided above the color filter or the like at a pixel region having smaller cell gap. With this structure, all spacers provided within a cell can substantially contribute to maintaining the gap.

A cell-specific dictionary is applied adaptively to adequate cells, where the cell-specific dictionary subsequently optimizes the handling of frequency-partitioned multi-dimensional data. This includes improved data partitioning with super cells or adjusting resulting cells by sub-dividing very large cells and merging multiple small cells, both of which avoid the highly skewed data distribution in cells and improve the query processing. In addition, more efficient encoding is taught within a cell in case the distinct values that actually appear in that cell are much smaller than the size of the column dictionary.

Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.

The invention provides a terahertz metamaterial device for detecting circulating tumor cells and a preparation method thereof. The device is mainly characterized with a stereo terahertz metamaterial,supplemented by a liquid diversion region. Only a small amount of blood sample is introduced from an inlet hole in a cover plate, and then blood cells of smaller size can be filtered according to structural features. Cancer cells of larger size are fixed at the sensitive positions, namely the clamping positions, of a metamaterial resonant structure. The cancer cells in the sample can be detected by comparing spectral changes before and after the sample is introduced. In addition, the device enables single separation of cancer cells, and can be used for subsequent biological study of individualcancer cells, so as to master the trait changes of each individual cancer cell stimulated by external conditions based on spectral analysis. Compared with the prior art, the device has high sensing sensitivity, and has remarkable effects when being used for fixed-point capture or large cell monitoring.

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.

An airfoil member can have a root end, a tip end, a leading edge, and a trailing edge. The airfoil member can include an upper skin, a lower skin, and a composite core member having a plurality of cells, an upper surface network of the cells can be bonded to the upper skin, a lower surface network of the cells can be bonded to the lower skin. The composite core can have a septum layer embedded in the cells that form the composite core, the septum layer being configured to provide tailored characteristics of the airfoil member.

The invention aims to provide an efficient culture method of CIK cells. The prepared CIK cell has the characteristics of high multiplication speed, large cell quantity, high cell viability, high killing capacity on cancer cells and the like. The efficient culture method mainly characterized in that a culture flask coated with laminin and fibronectin is used for culturing PBMCs (peripheral blood mononuclear cells), and the cells can be in a half-adherence state by means of the absorption effect of the two kinds of protein, accordingly, the cells can be stimulated by various factors, and the two kinds of protein can promote CIK cell multiplication; and with the adoption of an anti-CD28 monoclonal antibody, the co-stimulation signal of a T cell subset in the CIK cells can be stimulated, and the activation of the CIK cells is promoted. So far, a research report on jointly using laminin, fibronectin and the anti-CD28 monoclonal antibody to prepare the CIK cells is absent. The method for increasing the cell number and improving the killing activity by adding the three factors during preparation of the CIK cells is firstly provided.

A method for performing a cell reselection in a system comprised of a hierarchy of cells, wherein cells of one layer of the hierarchy have a size that differs from cells of another layer of the hierarchy. The method includes steps of (a) identifying to a user equipment a layer to which individual cells in a neighbor cell list are associated; (b) when performing neighbor cell measurements for reselection purposes, avoiding a measurement of cells in the list that are larger than a current serving cell unless a Cell Selection (S) parameter falls below a search threshold parameter (Ssearch), and is greater than zero; and (c), if S is less than or equal to zero, and no cell reselection to a better cell is in process, beginning a measurement of neighbor cells without regard for their hierarchical level. If the user equipment has made cell reselection to N different cells on the same hierarchical layer within a time Tmax, the user equipment initiates a reselection procedure for larger neighbor cells. This procedure accommodates user equipment that is moving at a relatively high speed through the cells. If the user equipment locates a larger cell, which fulfills cell reselection criteria Sn > 0 and Qn > Qs+Qoffsets,n+Qhyst for a period of time Treselection, cell reselection to the larger cell is made. Furthermore, if the user equipment performs a cell reselection to the larger cell, the user equipment does not attempt to reselect to a smaller cell within a time X, unless an immediate cell evaluation is triggered, wherein the time X can be pre-specified, or can be set by the network.

A method of treating large cell lung cancer in a subject in need thereof is provided. The method comprising administering to the subject a therapeutically effective amount of a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof, thereby treating the large cell lung cancer in the subject.

The invention discloses a cervical cell pathological slice image pathological cell identification method and system based on cell mass, which comprises the following steps: extracting a single cell mass region on the cervical pathological slice image; extracting the single cell mass region on the cervical pathological slice image; counting the size of the cell mass region, and treating the cell mass whose size exceeds a predetermined threshold value as a super-large cell mass; the superlarge cell mass being divided into daughter cell mass; the region to be recognized being obtained by boundaryfilling treatment of the cell cluster region other than the super-large cell cluster and the daughter cell cluster obtained by splitting; a lesion cell being identified in that region to be identified. The invention aims at the whole slice image of the massive pixels, takes the cell cluster as the processing and recognition unit, instead of the conventional image partition and fusion frame, whichis more suitable for the characteristics of the cervical cell pathological slice image, and improves the efficiency and the precision at the same time.

The present invention provides a signal transducer specifically expressed in mouse mast cells that has the amino acid sequence of SEQ ID No. 2, a signal transducer specifically expressed in human mast cells that has the amino acid sequence of SEQ ID No. 4, polynucleotides encoding these proteins, an expression vector involving these polynucleotides, transformed cells induced by these expression vectors, and antibodies against the foregoing proteins. The signal transducer provided in the present invention is useful for screening of novel medicines against allergic diseases.

The invention discloses a tumor cell mass separation and filtration device which comprises a bottom plate, two support plates are symmetrically and fixedly connected to the upper side of the bottom plate, and fixed boxes are fixedly connected to the upper ends of the support plates. The invention further provides a use method of the tumor cell mass separation and filtration device. The method comprises the following steps that S1, a double-head motor is started, and drives an eccentric wheel to rotate, the inertia of the eccentric wheel can make a transverse plate to move up and down, and thetransverse plate drives two first sliding rods to slide back and forth, and then a connecting block with a sieve mechanism to move up and down. The device is provided with a vibration mechanism and the sieve mechanism so that a first filter screen can jitter up and down and left and right, manual stirring can be omitted, the burden of operators is reduced, and cell damage is reduced; meanwhile, flowing is more smoothly, the device is provided with the two filter screens arranged vertically, large cell masses are removed for the first time, after separation, the cell masses are more uniform, the second filter screen on the lower layer, damaged single cells can be removed, and the separation quality is guaranteed.

Techniques for organizing a cell library permit a large number of cells. To improve design accuracy using cell libraries, very large cell libraries are needed. However, optimization tools are not able to use very large cell libraries directly, since their results suffer. Very large cell libraries are organized into sublibraries that are adapted to be processed by optimization tools. This allows improvement in the design quality of integrated circuits, while allowing the designs to be processed by optimization tools.

The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBSAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAq particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.

The invention discloses a method of producing a foot-and-mouth disease vaccine by using a large-scale high-density BHK-21 cell adherent culture technology. The method comprises the following steps of: 1) thawing and primarily amplifying cells; 2) conducting large-scale and high-density cell culture in a medium-volume bioreactor, and after the culture is completed, diluting the high-density cell sap in a large cell culture pot; and 3) inoculating foot-and-mouth disease seed virus, harvesting all virus suspension under proper conditions, and preparing the foot-and-mouth disease vaccine through the following steps. The process method is free from the process of acclimating the adherent cells into suspension cells; the necessarily gradual amplification process in the existing technology of preparing the foot-and-mouth disease vaccine by conducting large-scale suspension culture of the BHK-21 cell can be avoided, the technology flow can be obviously simplified, the production cycle can be shortened, the escape of the foot-and-mouth disease virus can be furthest reduced, and the pollution possibility can be decreased.

The invention provides a micro-fluidic chip-based multiple-organ tumor targeting drug testing platform and application thereof. A micro-fluidic chip consists of an upper layer chip, a lower layer chip and a porous filter membrane, wherein the upper part of the porous filter membrane is connected with the upper layer chip, and the lower part of the porous filter membrane is connected with the lower layer chip; the structure of the upper layer chip is of a large cell culture chamber, and the structure of the lower layer chip is of independent multiple channels, wherein the number of the channels is 1-9. A construction method of the micro-fluidic chip-based multiple-organ tumor targeting drug testing platform comprises the following steps: (1) manufacturing and modifying the chip; (2) inoculating and culturing cells in the upper layer chip; (3) inoculating and culturing cells in the lower layer chip. The upper layer chip is inoculated with hepatocytes so as to simulate the process of liver metabolism in a body, the lower layer chip is inoculated with different tumor cells and normal tissue cells, so that the effect of drugs, subjected to liver metabolism, on the different cells can be observed, and the effects of drugs, subjected to liver metabolism, on multiple groups of cells can be also observed; therefore, the screening and testing of the tumor targeting drugs can be realized, and the medicine formation rate of the screened drugs is greatly improved.

The invention is a cell filter, a micro-fluid device for cell filtration and separation. It uses a closed fluid channel as a cavity, two ends of the cavity are provided with an fluid input port and an fluid output port, respectively, there is a cell filtering device, namely a cell particles stopper arranged between the two ports, and there is a fluid tank beside the stopper. The cell stopping mechanism can stop larger cells in the fluid and make them gather in the fluid tank, and they are caught by the related antibodies. By the external physical or chemical action, it makes them broken or inactivated, thus achieving the cell filtration. It can be used in eliminating tumor cells in the blood and has important application prospect in reducing transfer danger of tumor cells for the tumor patient operated, inhibiting proliferation and recrudescence of the tumors, improving the curative effect of tumor patients, etc.

The invention discloses a tissue-like manufacture die based on a spheroid elementary unit and a preparation process. The invention mainly comprises that small balls with internal channels are used as elementary units to construct a compound body of brackets, cells and growth factors, i.e. millimeter-sized small balls with internal channels are constructed through a pressing method, then larger cell and bracket like tissues are constructed through a plurality of small ball elementary units, and the prepared compound can be further used for culturing in vitro or being directly implanted into the body.

Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.