The invention belongs to veterinary medicine preparation technology and provides an emulsified isoflurane compound anesthetic for dogs and a preparation method thereof. The emulsified isoflurane compound anesthetic for the dogs is formed by isoflurane, citric acid fentanyl citrate, lidocaine hydrochloride and 30% of fat emulsion. The isoflurane, the citric acid fentanyl citrate and the lidocaine hydrochloride are dissolved in the 30% of fat emulsion and then potted and sealed in ampoule bottles, after full oscillation, water bath and sterilization, the emulsified isoflurane compound anesthetic for the dogs is obtained. The anesthetic is rapid in anesthesia induction, safe and effective in the process of anesthesia, strong in anesthesia controllability, small in toxic and side effects, convenient to use, and especially suitable for complex operations or long-time operations, and the dogs can revive rapidly.