13 results about "Isoflurane" patented technology

The invention belongs to the technical field of pressure sore treatment, and discloses an experimental test method for treating stress injury based on danshen emodin, which comprises the following specific steps: step 1, preparing experimental materials, selecting a healthy SD (Sprague Dawley) rat, and preparing a clean and sterile magnet and a shaver; the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1 part of isoflurane, 1 part of iodophor, 1 part of Vaseline, 1 part of Rhapontigenin and 1 part of resveratrol. The method comprises the following steps: modeling a stress injury model for an SD (Sprague Dawley) rat so as to simulate a stress injury disease, then respectively smearing three medicines, i.e., Danjie emodin, resveratrol and vaseline, tracking the healing condition of a stress injury wound, and finally performing ELISA (Enzyme-Linked Immunosorbent Assay) and Hamp; according to the present invention, the detection analysis is performed through the dyeing E, and the results show that the rheum emodin has good treatment effect on the stress injury, can reduce the expression of the proinflammatory factor, can promote the wound healing, has characteristics of environmental protection and low cost, and provides the new research direction for the treatment of the stress injury.

The invention provides a polymer framework ink, a polymer framework and cell-loaded gel collaborative 3D printing method and a structural body. The polymer framework ink contains a high-molecular polymer, an organic solvent and organic modified synthetic flaky silicate, wherein the organic solvent comprises a first solvent and a second solvent, the first solvent is an ethanol aqueous solution and / or absolute ethyl alcohol, and the second solvent is methyl acetate and / or isoflurane. The polymer framework ink provided by the invention is rapidly cured through direct volatilization of the organic solvent; the flake silicate is synthesized through organic modification, so that higher fidelity is obtained, and the effect of rapid shaping is achieved while curing is carried out; different sizes of pores in the polymer filaments can be obtained by controlling the temperature difference between the polymer frame ink and the environment; finally, collaborative printing of the polymer framework and the cell-carrying gel can be realized under mild conditions, and the activity of cells is ensured.

The invention relates to the technical field of neutrophile granulocyte separation, in particular to a mouse neutrophile granulocyte separation method which comprises the following steps: (1) anesthetizing and disinfecting a mouse; (2) tissue sampling; (3) tissue digestion; (4) preparing a cell suspension; (5) purifying cells; (6) cell culture; according to the method disclosed by the invention, isoflurane is adopted in the anesthesia disinfection step, so that central nervous activity of mice can be rapidly inhibited, tissue damage caused by stress reaction is avoided, and chemical stimulation can be reduced while body surface microorganisms are cleared through combination of ethanol disinfection and sterile normal saline flushing, and aortic tissues with intact activity are provided for subsequent material taking. In the compound enzyme digestive juice, collagenase I can specifically degrade aortic wall collagenous fibers, trypsin dissociates adhesion connection between cells, neutral protease assists in decomposing elastic fibers, and DNA enzyme I is supplemented to inhibit cell aggregation, so that the contradiction between incomplete digestion and large cell damage of the traditional enzyme juice is effectively solved.

This invention relates to the field of synthetic biology, specifically to an oxygen methyltransferase and its application in the biosynthesis of isozyridine and aziridine. This application identifies the core gene elements of the isozyridine biosynthetic pathway—the oxygen methyltransferase genes SgOMT3 and Ese08G000662.t1—which encode proteins capable of catalyzing the conversion of fraxinus to isozyridine. Based on the oxygen methyltransferase of this application, combined with the isozyridine metabolic pathway, isozyridine can be synthesized in bacteria, yeast, algae, and plant substrates. This application provides an artificial synthetic pathway for isozyridine, offering a theoretical basis for the efficient, green, and low-carbon production of isozyridine through biosynthesis.

The invention belongs to the technical field of bioengineering, and discloses a method for constructing a bone-affinity mouse hepatoma cell line with bone metastasis characteristic, which comprises the following steps: digesting a mouse hepatoma cell line Hepa1-6 with pancreatin for 5 minutes, adding into a complete medium to stop digestion, centrifuging for 5 minutes at normal temperature by 300g, resuspending into 1 million cells / 100mu l by using PBS, and transferring into a 1.5 ml EP tube; after the mouse is anesthetized, shaving off hair at the chest of the mouse by using a shaver with the abdomen facing upwards, fixing the mouse on an operation table, sucking 100 [mu] l of cell suspension by using an insulin needle, inserting the needle from the intersection point of the left-side xiphoid process and the lower costal margin, and slowly injecting cells after active blood return is seen; the bone metastasis formation condition of the lower limb bone Hepa1-6 of the mouse is monitored regularly through an SPECTRAL Lago X small animal living body imaging system, isoflurane is used as an anesthetic, and it is guaranteed that the shooting time and the exposure condition of each time of monitoring are consistent; after obvious bone metastasis is formed, obvious mobility inconvenience of the mouse is observed; through multi-generation in-vivo screening, the mouse liver cancer cell line with strong bone affinity is obtained.

The application belongs to the technical field of medicine synthesis, and provides a new preparation method of isoflurane. The method uses hypochlorite and a strong acid as a chlorinating reagent to perform chlorination, which avoids the problems of low conversion rate and excessive chlorination to generate more impurities caused by chlorine gas chlorination. The reaction is more moderate, economical, environment-friendly and high-yield, and is suitable for industrial production.

The utility model discloses an etherification reaction rectification device for isoflurane, and belongs to the field of rectification devices.The etherification reaction rectification device comprises a connecting pipe, a mounting device is arranged on the connecting pipe and comprises a supporting plate, the supporting plate is fixedly connected to the outer wall of the connecting pipe, a sleeve is fixedly connected to the top of the supporting plate, and a sliding rod is slidably connected to the inner wall of the sleeve; the top of the sliding rod is fixedly connected with a fixing rod, the bottom of the fixing rod is fixedly connected with an inserting rod, a sliding groove is formed in the front face of the sleeve, the front face of the sliding rod is rotationally connected with a pulley through a bearing, and the sliding rod movably penetrates through the supporting plate and extends to the bottom. The bottom of a connecting plate at the bottom of a sliding rod is pushed, the sliding rod moves towards the top, a spring is stretched, the sliding rod drives an inserting rod to reset and be installed in an inserting hole, the stability and safety of the reaction kettle pipeline in the using process are ensured through spring resetting and clamping mechanisms, and the reaction kettle pipeline is convenient to replace.

The utility model discloses a chlorination reaction washing device for isoflurane, and belongs to the field of isoflurane processing.The chlorination reaction washing device for isoflurane comprises supporting legs, a device body is fixedly installed at the tops of the supporting legs, a water storage tank is arranged at a water outlet of the device body, a filter is arranged in the water storage tank, and a water outlet of the device body is provided with a water outlet. A moving assembly is arranged on the filter and comprises a first supporting frame, a motor is fixedly connected to the top of the first supporting frame, a rotating plate is arranged at the front end of the motor, a first gear is hinged to the front end of the upper portion of the rotating plate, and a second gear is meshed with the bottom of the first gear. By arranging the moving assembly, the filter can be driven by the moving assembly to move up and down in the water storage tank, impurities contained in washing liquid discharged by the device main body can be cleaned by the filter, and secondary utilization of the washing liquid is facilitated.

This application relates to the field of adhesive technology, and provides a polyurethane adhesive and its preparation and application methods. The polyurethane adhesive includes a first component and a second component. The raw materials of the first component include a polyurethane prepolymer, isocyanate, pigments and fillers, and a thixotropic agent. The raw materials of the second component include a polyol, a dehydrating agent, pigments and fillers, a first catalyst, and a second catalyst. The polyurethane prepolymer is obtained by reacting a low-activity isocyanate monomer and a chain extender. The low-activity isocyanate monomer is selected from at least one of isoflurane diisocyanate, hexamethylene diisocyanate, dicyclohexylmethane diisocyanate, and hexamethylene diisocyanate trimer. The first catalyst is selected from at least one of organotin catalysts and organobismuth catalysts. The second catalyst is selected from at least one of amine catalysts.

The invention discloses a flame-retardant high-resilience polyurethane foam packaging material and a preparation method thereof, and belongs to the technical field of new materials, the flame-retardant high-resilience polyurethane foam packaging material can be used for packaging precise instruments, precious equipment and the like, and the flame-retardant high-resilience polyurethane foam packaging material is prepared by foaming a flame-retardant black material A and a flame-retardant white material B to obtain a body material and then soaking the body material in halloysite branched polyethyleneimine and polyacrylic acid suspension in sequence; the A is prepared by mixing a flame retardant, imidazolidinyl urea, isobornyl acrylate and hydroxyethyl acrylate, wherein the flame retardant is prepared by mixing isophorone diisocyanate, benzoguanamine, phosphonitrilic chloride trimer and triethylamine; the component B is prepared by mixing polyester polyol, two polyether polyols, silicone oil, glycerol, water, pentamethyldiethylenetriamine, stannous octoate and a flame retardant F; the F is formed by mixing a ZIF solution G and a flame-retardant solution H; g is formed by mixing 2-methylimidazole and an ethanol solution of cobalt nitrate hexahydrate; h is prepared by mixing and reacting a diethylenetriamine ethanol solution with a mixed solution of ethanol, diphenyl chlorophosphate and triethylamine. The environment-friendly flame-retardant rubber has good flame retardance and resilience and is environment-friendly.

This application relates to the field of wiring harness protection materials, and discloses a wiring harness protection material for a new energy vehicle motor controller and its preparation method. The wiring harness protection material for a new energy vehicle motor controller is made by impregnating a base fabric layer with a polyurethane adhesive and then curing it. The polyurethane adhesive is prepared from the following raw materials in parts by weight: 70-80 parts of silane-modified polyurethane prepolymer, 22-28 parts of conductive filler, 4.5-7.5 parts of dispersant, 1-3 parts of antioxidant, 2-4 parts of curing agent, and 80-100 parts of solvent. The silane-modified polyurethane prepolymer is prepared by reacting isoflurane diisocyanate, N-(p-maleimide-phenyl)isocyanate, 3-isocyanatopropyltriethoxysilane, polyether polyol, and a catalyst, with DMF as the solvent. The wiring harness protection material of this application, when applied to a new energy vehicle motor controller, exhibits good electromagnetic shielding stability under long-term high-temperature conditions and good flexibility and bending resistance.

The invention relates to the technical field of experimental medicine, and discloses a controllable hierarchical thoracic and abdominal knocking injury animal model establishment method, which comprises the following steps: S1, selecting a healthy beagle; s2, trachea cannula vein general anesthesia is conducted, a medicine injection cannula is connected with a breathing machine, and suction of 1% of isoflurane is maintained; s3, lying on the back and fixing on an experiment table with a thoracico-abdominal exposure hole; s4, a DZHE-01 injury platform is used for being aligned with the chest and the abdomen; s5, regulating pressure, grading and causing injury, and recording an overpressure curve; s6, recording indexes according to time after injury, and performing detection and image inspection. According to the method for establishing the controllable graded chest and abdomen knocking injury animal model, a healthy adult beagle is supine and fixed to an experiment table with chest and abdomen exposure holes, the upper edge of the sternum is exposed to symphysis pubis, a 5 mm nitrile rubber pad is laid on the table top, a lead plate is used for shielding the head and neck, rubber protection plates are used for shielding the limbs, and DZHE-01 platform shock waves only act on the chest and the abdomen; by combining light single-time, middle 1-2-time and heavy two-time grading injury, multiple thoracic and abdominal organ injuries are simulated, and the problem that an existing model is single in part is solved.