The present invention belongs to the field of
biology, and relates to applications of serum ssc-miR-194b as a molecule marker for detection of piglet intestinal tract
stress injury. According to the present invention, the
nucleotide sequence of the serum ssc-miR-194b is represented by SEQ ID NO.1, the stem-loop primer
nucleotide sequence required by the reverse transcription of the ssc-miR-194b is represented by SEQ IDNO.2, the
forward primer nucleotide sequence required by the amplification of the ssc-miR-194b is represented by SEQ ID NO.3, and with the primers, the diagnosis on the weaned piglet intestinal tract
stress injury can be achieved, and the diagnosis method comprises serum
total RNA extraction, micromolecule
RNA separation, cDNA synthesis, and PCR amplification and identification; and the guide is provided for the prevention, the diagnosis and the treatment of the weaned piglet intestinal tract
stress injury, and the new molecule marker is provided for the marker-assisted selection of the pig anti-stress strain.