21 results about "Cartilage formation" patented technology

Compositions and methods for improving chondrogenesis, or treating osteoarthritis, or treating joint degradation or treating an osteochondral defect in a subject.

The present invention discloses the use of Avn Anthramides C (Avn C) as an inducer of chondrocytic differentiation in Mesenchymal Stem Cells (MSCs), providing a promising approach for regenerative cartilage therapy. By employing Avn C treatment, MSCs exhibit enhanced chondrocytic differentiation and elevated expression of key specific chondrogenic extracellular matrix (ECM) factors, notably Collagen Type II (COL2A1). Furthermore, when Avn C is used in combination with Chondroitin Sulfate (CS), a synergistic effect is observed, leading to upregulation of the transcriptional activity state of SOX9, a key regulator of chondrogenesis. The disclosed invention shows significant improvements in mRNA gene expression (ACAN, LRP1, SOX9), cellular protein levels (COL2A1, SOX9, LRP1), and functional outcomes, as evidenced by the secretion of extracellular matrix proteins (FSTL-1, BGH3, CO1A1, CO1A2, and CO3A1).

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

This application proposes a probe assembly, reagent kit, and their applications for detecting knee osteoarthritis, belonging to the field of biomedical detection technology. It addresses the technical problems of existing methods for detecting the risk or pathogenesis factors of knee osteoarthritis, such as slow speed, low throughput, and high cost. The probe assembly includes 112 probe sequences for detecting 56 target genes. The nucleotide sequences of these 112 probe sequences are shown in SEQ ID NO.1~SEQ ID NO.112, with at least two probe sequences corresponding to each target gene. The 56 target genes include 20 genes related to bone metabolism and cartilage formation, 19 genes related to inflammation, 8 genes related to extracellular matrix, and 9 genes related to drug metabolism and efficacy. This application features high throughput, speed, and accuracy. It can be applied to genetic risk assessment of knee osteoarthritis.

The invention belongs to the technical field of biological medicine, and discloses application of miRNA Let-7a-5p in a medicine for promoting cartilage formation, the invention provides the effect of miRNA let-7a-5p in cartilage formation / generation for the first time, and experiments prove that miRNA let-7a-5p can remarkably promote cartilage cell proliferation, enhance the activity of cartilage cells and remarkably improve matrix secretion of cartilage cells, so that the effect of promoting cartilage formation / generation is achieved. The expression of type II collagen is increased (the type II collagen is the main component of the cartilage matrix). Meanwhile, the miRNA let-7a-5p can also regulate other genes (such as NBL1 and DUSP7 genes) to inhibit cartilage ossification, and is expected to be applied to cartilage injury repair and osteoarthritis treatment, cartilage regeneration promotion and cartilage injury repair.

Compositions and peptides for enhancing formation of cartilage in a site of a cartilage injury or defect, or joint condition, and methods of the use of the compositions and peptides in such treatments. Examples of the sites may include a knee, ankle, wrist, shoulder, elbow, patella, hip, vertebrae, femoral head, temporomandibular joint, glenoid of the scapula, jaw, and growth plate.

The invention relates to the technical field of biological medicines, in particular to application of biotin medicines and application of a biotin sustained release preparation. Biotin drugs are used for preparing drugs for promoting cartilage formation, retarding osteoarthritis progress and inhibiting cartilage loss, and can effectively relieve and treat osteoarthritis.

This application relates to the field of biomedical technology, and in particular to the application of biotin-based drugs and biotin sustained-release formulations. Biotin-based drugs are used to prepare medications that promote cartilage formation, slow the progression of osteoarthritis, and inhibit cartilage loss, and can effectively relieve and treat osteoarthritis.

This invention relates to a recombinant human secretory DDRGK1-Fc and its applications. Through experimental verification, the invention synthesizes recombinant human secretory DDRGK1-Fc, which has good bone-promoting function and is applied to the preparation of drugs for treating bone and joint-related diseases, promoting cartilage formation, and promoting intervertebral disc formation. This opens up new ideas for the treatment of bone and joint diseases and has obtained relatively solid scientific evidence.

The invention relates to the technical field of biological medicine, in particular to a traditional Chinese medicine composition for promoting regeneration of hyaline cartilage and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-30 parts of prepared rehmannia root, 10-30 parts of astragalus membranaceus, 10-20 parts of Chinese yam, 10-20 parts of cistanche deserticola, 5-15 parts of angelica sinensis, 5-15 parts of corydalis tuber, 5-15 parts of spina date seed and 3-10 parts of safflower carthamus. The traditional Chinese medicine composition has the traditional effects of tonifying kidney and replenishing essence, tonifying qi and activating blood, and activating meridians to stop pain, and is proved to be capable of effectively relieving oxidative stress of a local microenvironment of joint injury and remodeling metabolic homeostasis of mesenchymal stem cells by up-regulating expression of HIF-1alpha and PLK1, so that regeneration of high-quality transparent cartilage is specifically induced, formation of fibrocartilage is inhibited, and the joint injury effect is improved. The composition is used for preventing or treating articular cartilage defects and osteoarthritis.

The invention provides a probe combination and a kit for detecting knee osteoarthritis and application of the probe combination and the kit, belongs to the technical field of biological medicine detection, and can solve the technical problems of low speed, relatively low detection flux, high detection cost and the like of the existing knee osteoarthritis risk or pathogenesis factor detection means. The probe combination comprises 112 probe sequences for detecting 56 target genes, the nucleotide sequences of the 112 probe sequences are respectively shown as SEQ ID NO.1-SEQ ID NO.112, and each target gene corresponds to at least two probe sequences; wherein the 56 target genes comprise 20 bone metabolism and cartilage formation related genes, 19 inflammatory response related genes, 8 extracellular matrix related genes and 9 drug metabolism and curative effect related genes. The method has the characteristics of high throughput, rapidness, accuracy and the like. The method can be applied to genetic risk assessment of knee osteoarthritis and the like.

The invention relates to regenerated cartilage based on hydrostatic pressure stimulation and a preparation method and application thereof. The method comprises the following steps: inoculating cells on a porous hydrogel scaffold, placing the porous hydrogel scaffold in a culture medium, performing pressure stimulation by adopting a hydrostatic pressure bioreactor, and performing in-vitro culture for a period of time to obtain the regenerated cartilage based on hydrostatic pressure stimulation. Key mechanical sensitive channels TRPV4 and PIEZO1 are up-regulated through hydrostatic pressure stimulation, a Ca < 2 + > dependent TRPV4 / PIEZO1-Ca < 2 + >-SOX9 mechanical transduction pathway is activated, cartilage formation is promoted, and hypertrophy and ossification are inhibited. Through verification, the bioreactor system is combined with the porous hydrogel scaffold, has good biocompatibility, controllable pore structure and mechanical property, and is suitable for cartilage cell culture. After in-vitro pre-culture for 4 weeks, the cartilage-like construct subjected to HP pretreatment shows a long-term stable regeneration effect in an animal model (such as a goat), and especially in an in-situ auricle microenvironment, inflammation and heterotopic ossification can be effectively inhibited, and an efficient in-vitro cartilage engineering strategy is provided for repairing of various cartilage injuries.

This disclosure contemplates methods of improving the growth of damaged cartilage. In certain embodiment, this disclosure relates to methods performing microfracture and applying components reported herein to provide marrow stimulation with improved cartilage regeneration. In certain embodiments, compositions and materials comprise fasudil optionally in combination with thrombin, aprotinin, and transforming growth factor-beta.

Pharmaceutical and / or cosmetic formulations containing artificial peptides and their medical uses are described, the artificial peptides being characterized by proline sequences that are ubiquitous in key stromal cell proteins involved in wound healing, bone and cartilage formation, and the maintenance of connective tissue in all vertebrates. This invention relates to a pharmaceutical formulation in gel form, comprising a. a substance comprising Pro-X-X-Pro-Y-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Y-Y-Pro-Y-Y-Y-Pro-Y-Y-Y-Y-Pro-X-X-Pro-X-Pro-Y-Y-Y-Y-Pro-X-X-Pro-Y-Y-Y-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-Y-Pro-X-X-Pro-Y-Pro-Y-Pro-X-X-Pro-Y-Pro-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-X ... Artificial peptides comprising the amino acid sequence of NO:1) and / or artificial peptides comprising or composed of the amino acid sequence Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-Pro-X-Pro-Pro (SEQ ID NO:2), wherein i) Pro is proline; ii) X is an amino acid selected from Ala, Ile, Leu, Met, Phe, Trp and Val; iii) Y is an amino acid selected from Asn, Cys, Gln, Ser, Thr and Tyr; b. cross-linked hyaluronic acid fibers (HA-XL) and c. linear hyaluronic acid fibers (HA).

Compounds, pharmaceutical compositions, and a method of treating hard tissue diseases and disorders are disclosed. The compounds may be a peptide and is structured to bind integrin αvβ3 expressed by osteocytes and by selective binding to the cell surface integrin on hard tissue forming cells regulate three-dimensional bone shape, cartilage formation and repair.

The invention discloses a preparation method and an application of a double-layer GelMA-AlgMA functional hydrogel scaffold loaded with a growth factor. Comprising the following steps: preparing a GelMA hydrogel precursor solution of composite KGN; an AlgMA hydrogel precursor solution of the composite BMP-2 is prepared; and 3D printing. The preparation method has the beneficial effects that the GelMA compounded with the KGN and the AlgMA compounded with the BMP-2 are combined into the double-layer stent, the KGN promotes cartilage formation, the BMP-2 promotes bone formation, growth factors are released in a targeted manner, and specific repair is performed aiming at different tissue layers of an osteochondral interface.

The present invention belongs to the field of medical technology, and in particular relates to the use of halofuginone in the preparation of a drug for treating heterotopic ossification. The present invention provides an application of halofuginone in the preparation of a drug for treating heterotopic ossification. Through imaging observation, histological section staining result observation, and vascular fluorescence staining observation, the positive control group had obvious bone hyperplasia in the Achilles tendon of mice, bone structure formation, obvious cartilage formation in the bone formation area, and obvious blood vessel formation in the Achilles tendon of mice with heterotopic ossification, while the halofuginone-treated group had no obvious bone hyperplasia, no obvious bone formation and cartilage accumulation, and no obvious blood vessel formation in the Achilles tendon of mice with heterotopic ossification. This shows that halofuginone has a good effect of improving bone formation, cartilage accumulation and blood vessel formation, that is, it shows that halofuginone has a good anti-heterotopic ossification effect.

The present invention provides 6-hydroxy-8-oxatricyclo[3.2.1.02,4]octane-2-carboxamide derivatives of Formula (1) wherein the variables are as defined herein. The present invention further provides pharmaceutical compositions comprising such compounds. The compounds are used for inducing chondrogenesis, in methods of treating or preventing joint damage, resulting from joint injury or arthritis, and for inducing hyaline cartilage production. The present description discloses the preparation of exemplary compounds as well as pharmacological data thereof (examples 1 to 82; tables 1 and 2). An exemplary compound is e.g. rac-(1R,2R,4S,5R,6S)-4-(2-fluoropyridin-4-yl)-6-hydroxy-N-(4-(trifluoromethyl)pyridin-2-yl)-8-oxatricyclo[3.2.1.02,4]octane-2-carboxamide (example 1).

The present invention provides modified monocytes, modified macrophages, pharmaceutical compositions comprising the modified monocytes or modified macrophages described herein and at least one pharmaceutically acceptable carrier or excipient. Uses of the modified monocytes or the modified macrophages for the treatment of musculoskeletal diseases and inducing cartilage formation are provided. Also disclosed herein are in vitro culture methods for generating the modified macrophages.

The invention relates to lactoferrin, and compositions comprising the same, for use in enhancing connective tissue in a subject, especially in enhancing bone strength and / or bone growth and / or cartilage formation and / or cartilage health.

The invention provides a serum-free culture medium and application thereof, the serum-free culture medium comprises bioactive substances and a basic culture medium, does not contain B-27 cell culture additives, and is simple in preparation method and high in safety; the bioactive substances comprise vitamin C or a derivative thereof, heparin or a salt thereof, a transforming growth factor-beta, a fibroblast growth factor and a platelet-derived growth factor, and the basic culture medium is a commercialized mesenchymal stem cell serum-free culture medium with definite components. The serum-free culture medium is simple in preparation method and high in safety, the primary culture proliferation effect of the tendon-derived mesenchymal stem cells can be improved, and the tendon differentiation capacity and the osteogenesis, cartilage formation and adipogenesis differentiation capacity of the mesenchymal stem cells can be improved. The types and concentrations of the five bioactive factors and the type of the basic culture medium are screened and optimized, the effect of the serum-free culture medium is further improved, and a prospect is provided for clinical treatment of motor system injury.