40 results about "Cartilage bone" patented technology

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

This invention provides a composition for increasing a body height of a patient with short stature or an individual other than patients with short stature. More specifically, the invention provides: a composition for increasing the body height of an individual comprising a guanyl cyclase B (GC-B) activator as an active ingredient, the composition being to be administered to an individual free from FGFR3 abnormality; a method for increasing the body height of an individual free from FGFR3 abnormality which comprises activating GC-B; a method for screening an agent for increasing the body height of an individual which comprises selecting an agent for increasing the body height using GC-B activity as an indication; and a method for extending a cartilage bone free from FGFR3 abnormality which comprises activating GC-B in an individual.

A production method for producing cell colony forming units in vitro from a mammalian tissue explant, the method comprises the steps of a) growing a piece of the mammalian tissue explant in a growth medium to obtain cell colony forming units from immature cells from the piece of explant, and b) harvesting cells from one or more of the cell colony forming units for use in Autologous Cell Implantation/transplantation methods. The mammalian tissue explant is selected from the group consisting of cartilage; bone such as, e.g., bone marrow; connective tissue; muscle tissue such as, e.g., smooth muscle tissue, heart tissue, liver tissue and skeletal muscle tissue; skin tissue such as, e.g., periosteum; mucosal tissue; brain tissue, pancreas tissue and blood vessels. In particularly, the mammalian tissue explant is cartilage, such as elastic, fibro, hyalin or articular hyalin cartilage. The cells obtained are suitable for use in autologous implantation/transplantation methods. In a specific embodiment, the cell obtained are chondroytes, especially for use in autologous chondrocyte implantation (ACI) methods.

The invention discloses cartilage deficiency prosthesis, a preparation method thereof and an integrated cartilage-bone repair material. The prosthesis is composed of collagen and proteoglycan based on the mass ratio of 1/3-3, wherein, the collagen is I-type collagen or II-type collagen, and the proteoglycan comprises glycosaminoglycan, chitosan or chondroitin sulfate; and natural cartilage matrixes are longitudinally arranged in the prosthesis. The integrated cartilage-bone repair material is made by compounding the prosthesis with a bone matrix material together. The cartilage defect prosthesis of an articular cartilage matrix structure of the invention can meet the repair need of articular cartilage deficiency, effectively improve repair rate of articular cartilage injury and lower disability incidence of a patient.

The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises the following steps: preparing cartilage acellular matrixpowder, and preparing bone tissue demineralized bone matrix powder; preparing gelatin or a collagen solution; preparing gelatin or collagen suspension containing a cartilage acellular matrix, and preparing gelatin or collagen suspension containing a demineralized bone matrix; preparing gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix; constructing a cartilage-bone integrated three-dimensional digital model through computer aided design, and forming a cartilage-bone integrated kernel grid framework through three-dimensional printing; fixing the cartilage-bone integrated grid framework in a cylindrical mould, pouring the gelatin or collagen suspension containing the demineralized bone matrix, preparing a bone phase scaffold, pouringthe gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix, preparing a junctional phase scaffold, pouring the gelatin or collagen suspension containing the cartilage acellular matrix again, preparing a cartilage phase scaffold, and preparing into the cartilage-bone integrated three-dimensional frozen scaffold.

The object of the present invention is to provide a non-fibrogenesis collagen material which has a sufficient strength and can be used as a cell culture substrate, a scaffold material for regenerative medicine (for example, material for tissue engineering of cartilage, bone, ligament, corneal stroma, skin, or liver), an implantation material (for example, wound dressing material, bone grafting material, hemostatic material, anti-adhesive material) or a carrier for drug delivery. The object of the present invention can be solved by a non-fibrogenesis collagen material characterized by comprising a fish-derived collagen.

The invention discloses a gel-poly(lactide-co-glycolide) (PLGA) two-phase gradient transition cartilage-bone repair material and preparation thereof. The material is cylindrical and consists of an upper material and a lower material, wherein the upper material is glycidyl methacrylate-modified carboxymethyl chitosan and gelatin; the lower material is porous PLGA. The preparation process comprises the following steps: preparing a gelatin microsphere serving as a pore-forming agent into a PLGA porous scaffold of which one end face is a conical concave surface; preparing a gel solution by using the modified chitosan and modified gelatin; dripping the gel solution on the PLGA porous scaffold of the conical concave surface, inverting, performing ultraviolet light cross-linking to obtain the gel-PLGA two-phase gradient transition cartilage-bone repair material. The material has high biocompatibility and biodegradability, is in a two-phase gradient transition structure, is tightly combined without drop and is simple in preparation process.

The invention discloses a cartilago articularis vitrification cryoprotectant and a cartilage preservation method. Each 1000ml of a vitrification cryoprotectant comprises 150-250ml of 99.9% dimethyl sulfoxide, 110-200g of acetamide, 70-120ml of 1,2-propylene glycol, 10-80g of polyethylene glycol, 20-100g of trehalose and the balance of deionized water. The cartilage preservation method comprises the following steps: soaking cartilage bone blocks at 4 DEG C by a gradient vitrification cryoprotectant sequentially for 10 minutes, packaging by a refrigerating pipe, putting into liquid nitrogen and storing; taking out before transplanting, rewarming, and carrying out water bath rewarming at 37 DEG C for 10 seconds; then putting cartilage bone blocks in a 10% saccharose solution and a 5% saccharose solution at 37 DEG C respectively, and remaining for 5 minutes; and finally washing by a phosphate buffer for 3 times so as to transplant and apply. According to the invention, the cartilago articularis tissues can be stored for a long time, the relatively high cell survival rate of cartilage is maintained, and the osteochondral graft preservation effect is enhanced; the cartilago articularis vitrification cryoprotectant and the cartilage preservation method are applied to the cartilage tissue library and the clinical cartilage transplantation.

The invention provides a bone and cartilage integrated repair support and a preparation method thereof. The bone and cartilage integrated repair support comprises a bone repair layer and a cartilage repair layer connected with the bone repair layer; the bone repair layer comprises a biodegradable synthetic polymeric compound and a powder component with osteogenic activity; and the cartilage repairlayer comprises a biodegradable synthetic polymeric compound and a powder component with a cartilage formation activity. The bone and cartilage integrated repair support can stimulate a defect part of cartilages and sub-cartilage bones in a targeted way and facilitates tissue regeneration. Further, the cartilage recovered layer of the support includes cartilage cells, so that cartilage repair canbe accelerated, and the cartilage repair quality is improved.

The invention belongs to the field of tissue engineering, and discloses a preparation method of a nano-fiber scaffold for promoting cartilage regeneration to solve the problem that Nell-1 serving as anovel cartilage-derived growth factor can promote cartilage bone cell functions, but is easily inactivated in the process of being integrated into a tissue engineering cartilage scaffold. The preparation method includes the steps: firstly, preparing Nell-1 supported chitosan nano-particle solution and collagen/poly (L-lactic acid)-copolymer (epsilon-caprolactone) mixed solution; secondly, preparing a PLLA-CL/collagen nano-fiber mesh by coaxial electro-spinning and dynamic liquid collection technology; finally, preparing the collagen/HA mixed nano-fiber scaffold by collagen and hyaluronic acid(HA). The operation method is simple, the prepared nano-fiber scaffold is good in biological compatibility, biological activity of the growth factor can be protected, the release time of the growth factor is prolonged, and cartilage repair and regeneration can be promoted.

The object of the present invention is to provide a collagen fiber membrane, which has sufficient strength and can be used as a cell culture substrate, a scaffold material for regenerative medicine (for example, material for tissue engineering of cartilage, bone, ligament, corneal stroma, skin, or liver), an implantation material (for example, wound dressing material, bone grafting material, hemostatic material, anti-adhesive material) or a carrier for drug delivery. The object of the present invention can be solved by a fish-derived collagen fiber membrane, characterized in that (1) a tensile strength is 30 MPa or more, (2) a density determined by the gravimetric method, is 0.4 g/cm³ or more, and (3) an average membrane thickness is 1 μm to 2 mm, and a variation in membrane thickness is plus or minus 30%, relative to the average membrane thickness.

The invention discloses a surface modification method of a bio-material promoting ossification and a growth factor controllable slow-releasing system composite multilayer membrane promoting the ossification. In particular, the invention relates to a biological multilayer membrane which is constructed on the surface of the bio-material in a layer-by-layer electrostatic self-assembly manner through a layer-by-layer self-assembly technology and a growth factor slow-controllable-releasing technology with casein and heparin as a negative electric macro-molecular layer and collagen and a growth factor as a positive electric macro-molecular layer, wherein the obtain the multilayer membrane is in a structure of a circulation of a casein layer/heparin layer and a collagen/growth factor layer. In the invention, the four biological macro-molecules having excellent physico-chemical properties are deposited onto a same substrate material alternatingly, so that not only is the biological activity of the growth factor protected, but also the load amount and slow releasing of the growth factor can be controlled and adjusted. In addition, the multilayer membrane is excellent in biological mineralization promoting and ossification promoting capabilities and meanwhile can improve adhesion, growth and proliferation of mesenchymal stem cells. The multilayer membrane can be widely used in tissue repair engineering of cartilage, bone, blood vessel, nerve, cardiac valve and the like.

The invention discloses a preparation method of bionic directional double-layer hydrogel for repairing bone/cartilage, and belongs to the field of a biomaterial. The preparation method comprises the following steps of respectively preparing a growth factor modified cellulose nanocrystalline PDA-CNC for inducing cartilage regeneration and a hydroxyapatite in-situ mineralization modified cellulose nanocrystalline CaP-CNC by utilizing an adhesion effect based on mussel-imitating polydopamine; and enabling the PDA-CNC and natural macromolecules having good biocompatibility to be made into an upper-layer hydrogel prepolymer solution. Based on the directional arrangement characteristic of the upper-layer hydrogel prepolymer solution under the action of shearing force, a force field is adjusted by means of injection extrusion and the like, so that cellulose nanocrystals are arrayed in a hydrogel matrix in an oriented manner. The CaP-CNC is added into the lower-layer hydrogel, so that the mechanical strength of the lower-layer hydrogel is improved, and the hydrogel has osseointegration. By adjusting technological parameters, the upper layer and the lower layer of a hydrogel matrix have different active ingredients, structures and mechanical properties, natural cartilage/bone tissue can be simulated, and integrated repair and regeneration of cartilage and bone tissue are facilitated.

The invention belongs to the technical field of medical treatment, and particularly relates to a tendon-bone combined three-phase scaffold prepared by fusion electrospinning three-dimensional printing. A method comprises the following steps: S1, cell cultivation; S2, bracket preparation; and S3, cell planting. The method can accurately control the fiber diameter and the printing path by using a fusion electrostatic spinning three-dimensional printing technology, thereby greatly improving the printing precision; by using a spinning solution containing cytokine microspheres for electrospinning printing, the electrospinning fibers containing a large amount of cytokines can be obtained; and through different three-dimensional printing path parameters, the tendon-cartilage-bone three-phase scaffold can be obtained by printing. The scaffold obtained by the method has a bone-cartilage-tendon tissue gradient structure, has high structure precision, carries a large amount of microspheres with cytokines for slow release, promotes cell proliferation repair, and is beneficial to tendon-bone combined and normal function recovery.

The invention discloses a cartilage-bone repair support with bionic gradient and a preparation method thereof. For the cartilage-bone repair support, a cartilage layer of an interwoven porous structure is prepared from sodium hyaluronate and chitosan, a subchondral bone layer of a porous structure is prepared from graphene oxide, chitosan and nano-hydroxyapatite, and structure, composition and function bionics of natural articular cartilage is realized by combining a coprecipitation method and a gradient refrigeration technology by referring to an in-situ bionic thorough. The obtained supporthas an upper layer of structure and a lower layer of structure, nano-hydroxyapatite presents change of bionic gradient distribution which is gradually increased from the cartilage layer to the subchondral bone layer; meanwhile, the cartilage layer in the upper layer is of a mutually penetrating interwoven hierarchical pore structure, the subchondral bone layer is of an array microtube structure which is axially arranged and is vertically through, the support material is excellent in mechanical performance, strong in interface bonding property and good in biological performance, and is expectedto become a novel composite material for treating cartilage-bone defect.

The invention belongs to the technical field of surgical patches, and provides an artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and a preparation method thereof. The method comprises the following steps: selecting PET yarns as an inner layer and a gelatin solution in which CTGF, KGN and induction factors of nano-hydroxyapatite are respectively dispersedas an outer layer; spraying the gelatin solution onto the PET yarns by using an electrostatic spinning technology so as to obtian CTGF yarns, KGN yarns and nano hydroxyapatite yarns, wherein the three kinds of yarns are all nanofiber yarns with a double-layer structure; and then performing weaving according to the direction of CTGF-KGN-nano hydroxyapatite to obtain a nanofiber yarn stent with a knitted double-layer structure, namely the artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure. The preparation method of the artificial rotator cuff patch capable of inducing formation of the tendon-bone gradient structure is simple, and the obtained three-section artificial rotator cuff patch can promote formation of the tendon-fibrous cartilage-bone gradient structure, and has good mechanical properties, biocompatibility and clinical application prospects.

The invention discloses an injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading. The injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation is a porous microsphere constructed by poly(4-hydroxybutyrate) and a copolymer thereof through a pore-foaming agent. The invention further provides a preparation method of the microsphere and application of the microsphere in bone defect repairing. The prepared porous microsphere has the mechanical property similar to that of an extracellular matrix, can be used for enhancing progenitor cell aggregation through the porous structure of the microsphere on the premise of without stem cell or growth factor loading, so that the internal repairing capacity of bones can be supported, and intracartilage ossification bone repairing can be completed through a single material.