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A gradient laminated composite supporting frame material based on bionic structures and its preparation method

A composite scaffold and gradient technology, applied in the field of biomedical materials, can solve problems such as unsatisfactory repair effect and poor repair effect of scaffold materials, and achieve adjustable degradation rate, good mechanical properties, and good biocompatibility Effect

Inactive Publication Date: 2008-10-01
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the use of these bracket materials can not get a good repair effect
For example, chitosan-collagen composite scaffold, although its biological activity is good, its repair effect is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Take 0.2 g of PLGA (LA:GA molar ratio is 50:50, molecular weight 10W) ​​dissolved in 2 ml of dichloromethane, add 2 g of NaCl particles (particle size 150-200 microns) to make holes, stir well, this is a solution A: Dissolve 0.17 g of PLGA in 2 ml of dichloromethane, disperse 0.03 g of nano-HA in 1 ml of dichloromethane to make a suspension, homogenize it by ultrasonic for 20 minutes, and then add it to the solution of PLGA in dichloromethane , add 2 grams of NaCl particles to make holes, stir well, this is solution B; take 0.10 grams of PLGA dissolved in 2 milliliters of dichloromethane, disperse 0.10 grams of nano-HA in 2 milliliters of dichloromethane to make a suspension, ultrasonic After homogenizing for 20 minutes, add it to the dichloromethane solution of PLGA, add 2 grams of NaCl particles to make holes, and stir evenly. This is solution C; inject the three solutions A, B, and C into the mold respectively, and wait for the three solutions After the solvent in the...

Embodiment 2

[0036] Take 0.2 g of PLGA (the molar ratio of LA:GA is 75:25, molecular weight 18W) is dissolved in 2 ml of dichloromethane, add 2.4 g of NaCl particles (particle size 150-400 microns) to make holes, stir evenly, this is a solution A: Dissolve 0.16 g of PLGA in 2 ml of dichloromethane, disperse 0.02 g of nano-HA and 0.02 g of β-TCP in 1 ml of dichloromethane to make a suspension, and add it to PLGA after ultrasonic homogenization for 20 minutes Add 2.4 g of NaCl particles to the dichloromethane solution, stir evenly, and this is solution B; dissolve 0.12 g of PLGA in 2 ml of dichloromethane, and disperse 0.06 g of nano-HA and 0.02 g of β-TCP in Suspension was prepared in 2 ml of dichloromethane, homogenized by ultrasonic for 20 minutes, added to the dichloromethane solution of PLGA, 2.4 g of NaCl particles were added to make holes, and stirred evenly, this was solution C; A, B, C The three solutions are injected into the mold respectively, and after the solvents in the three s...

Embodiment 3

[0039] Take 0.1 g of II collagen, put it into 2 ml of 1% acetic acid aqueous solution, stir at room temperature for 2 hours to completely dissolve, add dropwise 2 ml of 1% hyaluronic acid aqueous solution, stir rapidly for 1 hour, add 1% hyaluronic acid solution Carbodiimide cross-linking agent to obtain cross-linked II collagen-hyaluronic acid aqueous solution, which is A solution; take 0.07 g of II collagen, put it into 2 ml of 1% acetic acid aqueous solution, stir at room temperature for 2 hours to completely Dissolve, add 2 ml of 1% hyaluronic acid aqueous solution dropwise, stir rapidly for 1 hour, add carbodiimide crosslinking agent with a concentration of 1%, to obtain crosslinked II collagen-hyaluronic acid aqueous solution, add 0.03 g nano- HA, ultrasonically homogenized for 20 minutes to obtain solution B; take 0.02 g of II collagen, put it into 2 ml of 1% acetic acid aqueous solution, stir at room temperature for 2 hours to completely dissolve, add dropwise 2 ml of 1...

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PUM

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Abstract

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method and a technology of a tissue engineering laminated composite support material for osteochondral defect repair. Background technique [0002] Articular cartilage defect is a very common disease, and its defect models include: I, less than 4 mm, a small area of ​​cartilage defect, which is a slight injury; II, greater than 4 mm, difficult to heal by itself, and requires external force; III, injury to the subchondral bone , because blood vessels in the bone will penetrate the subchondral bone and enter, leading to cartilage necrosis and fibrous tissue growth. The most common is the third type of injury. Due to the special tissue structure and biological characteristics of articular cartilage, although there are many known methods for treating articular cartilage defects, including: subchondral bone drilling, electrical stimulation, laser, drug an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46
Inventor 张胜民鲁华周为
Owner HUAZHONG UNIV OF SCI & TECH
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