71 results about "Subchondral bone" patented technology

An ankle joint replacement prosthesis comprises a tibia prosthesis assembly and a talus prosthesis, the tibia prosthesis assembly comprises a tibia prosthesis body and a cushion block, the tibia prosthesis body and the cushion block are in an arc shape with the middle bent upwards on the sagittal plane, the cushion block is arranged at the bottom of the tibia prosthesis body in a sleeving mode, and the surface of the upper portion of the tibia prosthesis body is a tibia joint face. The surface of the lower portion of the cushion block is a first articular surface, the surface of the upper portion of the talus prosthesis is a second articular surface, the second articular surface is in an arc shape with the middle bent upwards on a sagittal plane, the surface of the lower portion of the talus prosthesis is a talus joint surface, and the first articular surface is matched with the second articular surface. When the prosthesis is implanted, only the articular cartilage and the subchondral bone need to be treated at the far end of the tibia, so that the bone at the far end of the tibia is reserved to the maximum extent, the anatomical integrity of the bone is guaranteed, the stability of the prosthesis is enhanced, the service life is prolonged, and the clinical operation effect is improved.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention relates to the technical field of medical image auxiliary diagnosis, and provides an intervertebral joint osteoarthritis image feature evaluation method based on deep learning, which can synchronously identify five types of FJOA image features such as joint space stenosis, osteophyte, hypertrophy, subchondral bone erosion and subchondral cyst, and improves the comprehensiveness and efficiency of evaluation. The nnU-Net model is adopted to perform high-precision segmentation on an intervertebral joint region, so that the positioning precision is effectively improved; by introducing a shared feature extraction network based on ResNet-18 and five parallel classification sub-networks, joint modeling of multi-scale semantic information is realized, and the recognition accuracy and generalization ability of the model are improved; according to the method, a Grad-CAM technology is combined to generate an activation thermodynamic diagram, a model interpretation basis is superposed to an original image, visual display of an evaluation result is realized, interpretability and clinical applicability of the model are enhanced, and FJOA image features in axial lumbar vertebra CT images from two central data sets can be quantitatively evaluated so as to comprehensively quantify individual FJOA image features.

Disclosed herein are methods of treatment of pain associated with osteoarthritis, pain associated with osteonecrosis, and pain associated with one or more subchondral insufficiency fractures; methods of improving physical function in a human subject with osteoarthritis, osteonecrosis, or one or more subchondral insufficiency fractures; and methods of treating osteoarthritis, or delaying or slowing the progression of osteoarthritis, or relieving a symptom of osteoarthritis, in humans with these diseases, comprising administering a therapeutically effective amount of a composition comprising a glycosylated p75NTR neurotrophin binding protein (NBP)-Fc fusion protein.

The invention provides a controllable degradation magnesium-based bionic biphasic stent as well as a preparation method and application thereof. The stent comprises a porous magnesium stent, hydrogel and a ceramic coating, the porous magnesium scaffold comprises a cartilage phase scaffold and a subchondral bone phase scaffold which are arranged from top to bottom; hydrogel is arranged on pores and the outer surface of the cartilage phase scaffold, and a ceramic coating is arranged on the surface of the subchondral bone phase scaffold. From the bionic angle, the three-dimensional communicated porous magnesium stent with gradient porosity is designed by simulating the structure, mechanics and other characteristics of joint tissues (upper cartilage and lower subchondral bone); meanwhile, according to the characteristics of joint tissue components, biological properties and the like, hydrogel (an upper cartilage phase) is filled in the stent, and a bioactive ceramic coating (a lower subchondral bone phase) is prepared on the surface of the stent, so that the degradation rate of the biphasic magnesium stent is regulated and controlled, and the cytotoxicity is reduced; meanwhile, the biocompatibility and the biological activity are improved, and endogenous cartilage-subchondral bone integrated repair is realized.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the femorotibial joint, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The present invention relates generally to minimally invasive, cost-effective, adaptable methods, systems, and devices used to repair anatomical joint conditions. The repair may be necessitated by trauma, disease or other conditions. The anatomical joint may specifically include mammalian joints such as the knee, shoulder, elbow, wrist, finger, hip, spine, toe and ankle, for example. The methods, systems, and devices disclosed herein include leveraging the significant (and often unappreciated) role the subchondral bone plays in the health status of the afflicted anatomical joint.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

The present invention demonstrates that isosakurabin can inhibit the formation and function of osteoclasts in vitro, protect the bone microstructure of the subchondral bone in mice with osteoarthritis (OA), reduce articular cartilage degeneration in OA mice, inhibit the formation of abnormal blood vessels in the subchondral bone marrow cavity during OA progression, and alleviate joint pain induced by abnormal osteoclast activation in OA mice. This invention demonstrates for the first time that isosakurabin has a therapeutic effect on osteoarthritis and reveals its mechanism of action.

A method for modeling temporomandibular joint osteoarthritis, 6-week-old rats are selected, the rats are confirmed to have entered an anesthetized state before surgery, the rat's mandibular incisors are first checked and confirmed to be normal, the first arm of the device is sleeved on one side of the mandibular incisor, and the palatal side of the maxillary incisor is made to touch the slope surface of the second arm of the device, the device is checked and confirmed to not affect the rat's mandibular movement and occlusion, not significantly raise the bite, have no early contact, and the upper and lower jaw relationship is relatively stable; the device and the mandibular incisor sleeved with the device are fixed; then the rat is put back into the cage, and soft food or powdered feed is given after waking up; the device is removed after being fed for 8 weeks or more. It is verified that the condylar process of the rat changes significantly, the overall condylar process is significantly reduced, the subchondral bone is destroyed, and the expression of the cartilage matrix is also reduced. Thus, a TMJOA rat model exhibiting mandibular retrusion is formed.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The invention relates to the technical field of biomedical materials, in particular to a super-lubricating composite material for bionic cartilage and subchondral bone and a preparation method of the super-lubricating composite material. The super-lubricating composite material is formed by compounding a hydrogel precursor, a polyvinyl alcohol aqueous solution and a functional filler under an alkaline condition, the hydrogel precursor is prepared by carrying out a reaction on sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and m-aminophenylboronic acid in deionized water, and the hydrogel precursor is prepared by carrying out a reaction on the sodium alginate, the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and the m-aminophenylboronic acid; the composite material prepared by the invention has excellent mechanical properties, elastic modulus of bionic cartilage and extremely low friction coefficient, and also has excellent lubricating stability and self-repairing function, so that dynamic repairing and lubrication maintenance of a damaged interface are realized; the material is particularly suitable for a bionic cartilage layer in femoral head necrosis area replacement, friction between an implant and original cartilage can be effectively reduced, cartilage repair is promoted, and the joint function is improved.

The invention discloses an RIP3-mediated osteoarthritis animal model as well as a construction method and application thereof. The construction method comprises the following steps: delivering an effective dose of a genetic tool (such as an adenovirus vector) capable of expressing RIP3 protein into a knee joint subchondral bone area of an experimental animal, and realizing continuous overexpression of RIP3 through regular operation, so as to induce pathological characteristics highly similar to human osteoarthritis. The model stably shows typical OA phenotypes such as limb pain sensitization, subchondral bone loss and bone microstructure degeneration, osteoclast number increase and overlying cartilage degeneration. The invention reveals that the natural process of occurrence and development of the osteoarthritis can be directly simulated through specific overexpression of the RIP3 in the subchondral bone for the first time, and a brand-new and valuable tool is provided for researching the pathogenesis of the osteoarthritis and screening and evaluating medicines and therapies for treating the osteoarthritis.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

The invention relates to an in-situ lubrication 3D printing Janus bone cartilage repair scaffold and a preparation method thereof.The scaffold is prepared through 3D printing and comprises a subchondral bone layer and a cartilage layer, the subchondral bone layer is prepared from GelMA and NAGA loaded nHA, and bone healing is promoted; the cartilage layer is provided with zwitterionic monomers, and in-situ lubrication is achieved. In-situ lubrication can be realized through lubrication modification of the cartilage layer scaffold structure, and bone healing is promoted by loading hydroxyapatite on the subchondral bone layer scaffold.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

The invention relates to the technical field of biomedical materials, in particular to a double-network hydrogel material for bionic cartilage and subchondral bone and a preparation method of the double-network hydrogel material. According to the double-network hydrogel material for the bionic cartilage and the subchondral bone, polyvinyl alcohol is adopted as a first network skeleton, and a high-toughness hydrogel structure is formed through a circulating freezing and thawing method; sulfobetaine methacrylate and a calcium chloride solution are introduced as a second network cross-linking system, so that the material is endowed with excellent hydrophilic lubrication performance; furthermore, liposome microspheres are compounded to form a self-renewal liposome boundary layer, so that the friction coefficient is remarkably reduced; the material has high strength, high toughness, low friction, self-lubrication and biocompatibility, is suitable for precise repair and functional reconstruction of osteochondral defects such as femoral head necrosis and the like, and has a wide clinical application prospect.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused 2024 / 064830 by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the metatarsal phalangeal joint or other joint of the foot or ankle, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.WO

The invention discloses a double-layer piezoelectric hydrogel stent for temporal-mandibular joint injury repair and a preparation method and application thereof.The piezoelectric hydrogel stent comprises an upper stent layer for cartilage repair and a lower stent layer for subchondral bone repair, and the upper layer and the lower layer both comprise a colloid component and a piezoelectric component; wherein the mass percent concentration of piezoelectric components in the upper layer of the piezoelectric hydrogel stent is lower than that of piezoelectric components in the lower layer of the piezoelectric hydrogel stent. The mechanical property and the gradient piezoelectric property of the double-layer piezoelectric hydrogel stent are matched with tissue regeneration at the temporomandibular joint, and cartilage-bone synergistic regeneration at the temporomandibular joint can be accurately regulated and controlled. The novel bionic hydrogel stent disclosed by the invention can remarkably improve temporal-mandibular joint injury and has a wide clinical application prospect.

This invention discloses a neutralizing nanobody targeting soluble interleukin-17 receptor D (sIL-17RD), its preparation method, and its applications. The heavy chain variable region (VHH) of this nanobody contains a heavy chain complementarity-determining region 3 (CDR-H3) that specifically binds to sIL-17RD, with a length of 10 amino acids and containing 2–6 lysine residues. This invention provides four nanobodies: CQ5, CQ13, CQ11, and CQ6, corresponding to well-defined CDR-H1, CDR-H2, and CDR-H3 sequence combinations. This nanobody can specifically bind to and neutralize the biological activity of sIL-17RD, reducing chondrocyte death, promoting proliferation, and maintaining extracellular matrix homeostasis by inhibiting the P2rx7-NLRP3 inflammasome-mediated chondrocyte pyroptosis pathway. This invention also protects the nucleic acid encoding this nanobody, the recombinant expression vector, the host cell, the pharmaceutical composition, and the preparation method. The nanobodies described can be used to prepare drugs for the prevention and treatment of inflammatory osteoarthritis and other osteoarthritis. They can inhibit synovitis, reverse cartilage and subchondral bone degeneration, and relieve pain, showing promising application prospects.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

An adaptive porous femoral head necrosis support rod comprises a head, a body, a temperature-sensitive bone conduction layer and a tail; the head, the body and the tail are coaxially connected in sequence, and the temperature-sensitive bone conduction layer covers the surface of the body; the shape of the head matches the cavity surface left after the removal of dead bone, and the head and the body are both porous structures; the tail is provided with a thread, and the thread groove is provided with a porous structure. The porous personalized adaptive femoral head necrosis support rod has strong personalized adaptation. The shape of the head matches the cavity surface left after the removal of dead bone in the patient's marrow core, can maximize the contact area of the head and the patient's subchondral bone, improve the ability of the support rod to resist pressure and support the subchondral bone, and effectively prevent collapse.

The invention provides application of a composition in treatment of knee osteoarthritis. According to the invention, two rat knee osteoarthritis models (the former is called as an ACLT-induced KOA model for short, and the latter is called as a papain-induced KOA model for short) are established by respectively utilizing methods of combining anterior cruciate ligament transection (ACLT) with running and injecting papain composite L-cysteine into an articular cavity; the composition provided by the invention is used for treating two rat KOA models. Results show that the composition can improve knee joint swelling of two KOA rats, increase the pain threshold, improve the abnormal gait state, relieve cartilage injury, inhibit ACLT-induced abnormal change of a KOA rat knee joint subchondral bone microstructure and papain-induced PGE2 content in KOA rat synovial tissue, and can be used for preparing a medicine for treating osteoporosis. The level of inflammatory factors in serum and joint lavage fluid of the two KOA rats is reduced, and the discovery shows that the KOA has important application in treatment of knee osteoarthritis.

The invention provides a 3D printing double-layer gradient composite scaffold and a preparation method and application thereof, the 3D printing double-layer gradient composite scaffold is prepared by adopting a multi-nozzle collaborative printing technology of a 3D printing system, and the 3D printing double-layer gradient composite scaffold comprises a cartilage repair layer and a subchondral bone repair layer. Wherein the cartilage repair layer and the subchondral bone repair layer are respectively prepared by cooperatively printing a thermoplastic polymer material and a photocurable natural polymer material mixed with cells and double drug-loaded nanoparticles through double nozzles of a 3D printing system. The distance between the printed microwires of the cartilage repair layer is smaller than that between the printed microwires of the subchondral bone repair layer. The double-layer gradient composite scaffold is integrally prepared by using a multi-nozzle collaborative printing technology of a 3D printing system, has good mechanical strength and biocompatibility, and can realize integrated repair of osteochondral defects.

The invention discloses a double-layer piezoelectric hydrogel scaffold for realizing articular cartilage injury repair through ultrasonic regulation, the piezoelectric hydrogel scaffold comprises a scaffold upper layer for cartilage repair and a scaffold lower layer for subchondral bone repair, the upper layer and the lower layer both comprise a colloid component and a piezoelectric component, wherein the mass percent concentration of piezoelectric components in the upper layer of the piezoelectric hydrogel stent is lower than that of piezoelectric components in the lower layer of the piezoelectric hydrogel stent. The mechanical property and gradient piezoelectric property of the double-layer piezoelectric hydrogel scaffold are matched with articular bone cartilage tissue regeneration, and cartilage-bone synergistic regeneration can be accurately regulated and controlled. The novel bionic hydrogel scaffold can significantly improve articular cartilage injury, and has a wide clinical application prospect.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the humeroradial joint or other joint of the elbow, wrist, or hand, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The invention discloses an oleanolic acid cubic liquid crystal nanoparticle temperature-sensitive gel preparation and application thereof. The preparation is prepared by dispersing oleanolic acid cubic liquid crystal nanoparticles in a temperature-sensitive gel matrix, the oleanolic acid cubic liquid crystal nanoparticles are prepared from oleanolic acid, phytantriol and a stabilizer, and the temperature-sensitive gel matrix is composed of poloxamer F127 and poloxamer F68. The preparation disclosed by the invention has good temperature-sensitive characteristic, slow release performance and joint lubrication function, and can effectively prolong the residence time of oleanolic acid in a joint cavity and improve the bioavailability of oleanolic acid. Experimental results show that the preparation can effectively relieve pain and depression-like behaviors related to knee osteoarthritis, inhibit cartilage degradation and subchondral osteosclerosis, regulate inflammatory factors and oxidative stress indexes and systematically improve the functions of hippocampus through a brain-bone axis, and has important clinical application value.

The invention discloses ROS response type oil-water two-phase hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials.The ROS response type oil-water two-phase hydrogel is characterized in that alendronate sodium and Ce < 3 + > form a hydrogel matrix, the hydrogel matrix degrades and reduces ROS of an inflammation part under the action of ROS, the alendronate sodium and Ce < 3 + > in soluble Ce < 3 + > salt form chemical cross-linked hydrogel, and the ROS response type oil-water two-phase hydrogel is prepared. The effect between Ce < 3 + > and alendronate sodium can be destroyed by ROS, because the oil-water two-phase hydrogel prepared by the invention has ROS responsiveness, the oil-water two-phase hydrogel is degraded in a high ROS environment, such as an inflammatory site, and can further release Ce < 3 + > with an ROS removal function, alendronate sodium with pharmacological activity, anti-inflammatory essential oil and a hydrophobic drug, so that oxidative stress is relieved, osteoclast is inhibited, and the effect of the alendronate sodium is inhibited. Subchondral bone repair is promoted, synovitis is inhibited, mesenchymal stem cells are induced to be differentiated into cartilage, and the synergistic effect of multiple mechanisms of oxidation resistance, inflammation resistance and repair is achieved.