47 results about "Subchondral bone" patented technology

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention relates to the technical field of medical image auxiliary diagnosis, and provides an intervertebral joint osteoarthritis image feature evaluation method based on deep learning, which can synchronously identify five types of FJOA image features such as joint space stenosis, osteophyte, hypertrophy, subchondral bone erosion and subchondral cyst, and improves the comprehensiveness and efficiency of evaluation. The nnU-Net model is adopted to perform high-precision segmentation on an intervertebral joint region, so that the positioning precision is effectively improved; by introducing a shared feature extraction network based on ResNet-18 and five parallel classification sub-networks, joint modeling of multi-scale semantic information is realized, and the recognition accuracy and generalization ability of the model are improved; according to the method, a Grad-CAM technology is combined to generate an activation thermodynamic diagram, a model interpretation basis is superposed to an original image, visual display of an evaluation result is realized, interpretability and clinical applicability of the model are enhanced, and FJOA image features in axial lumbar vertebra CT images from two central data sets can be quantitatively evaluated so as to comprehensively quantify individual FJOA image features.

The invention provides a controllable degradation magnesium-based bionic biphasic stent as well as a preparation method and application thereof. The stent comprises a porous magnesium stent, hydrogel and a ceramic coating, the porous magnesium scaffold comprises a cartilage phase scaffold and a subchondral bone phase scaffold which are arranged from top to bottom; hydrogel is arranged on pores and the outer surface of the cartilage phase scaffold, and a ceramic coating is arranged on the surface of the subchondral bone phase scaffold. From the bionic angle, the three-dimensional communicated porous magnesium stent with gradient porosity is designed by simulating the structure, mechanics and other characteristics of joint tissues (upper cartilage and lower subchondral bone); meanwhile, according to the characteristics of joint tissue components, biological properties and the like, hydrogel (an upper cartilage phase) is filled in the stent, and a bioactive ceramic coating (a lower subchondral bone phase) is prepared on the surface of the stent, so that the degradation rate of the biphasic magnesium stent is regulated and controlled, and the cytotoxicity is reduced; meanwhile, the biocompatibility and the biological activity are improved, and endogenous cartilage-subchondral bone integrated repair is realized.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the femorotibial joint, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The present invention relates generally to minimally invasive, cost-effective, adaptable methods, systems, and devices used to repair anatomical joint conditions. The repair may be necessitated by trauma, disease or other conditions. The anatomical joint may specifically include mammalian joints such as the knee, shoulder, elbow, wrist, finger, hip, spine, toe and ankle, for example. The methods, systems, and devices disclosed herein include leveraging the significant (and often unappreciated) role the subchondral bone plays in the health status of the afflicted anatomical joint.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

A method for modeling temporomandibular joint osteoarthritis, 6-week-old rats are selected, the rats are confirmed to have entered an anesthetized state before surgery, the rat's mandibular incisors are first checked and confirmed to be normal, the first arm of the device is sleeved on one side of the mandibular incisor, and the palatal side of the maxillary incisor is made to touch the slope surface of the second arm of the device, the device is checked and confirmed to not affect the rat's mandibular movement and occlusion, not significantly raise the bite, have no early contact, and the upper and lower jaw relationship is relatively stable; the device and the mandibular incisor sleeved with the device are fixed; then the rat is put back into the cage, and soft food or powdered feed is given after waking up; the device is removed after being fed for 8 weeks or more. It is verified that the condylar process of the rat changes significantly, the overall condylar process is significantly reduced, the subchondral bone is destroyed, and the expression of the cartilage matrix is also reduced. Thus, a TMJOA rat model exhibiting mandibular retrusion is formed.

The invention relates to the technical field of biomedical materials, in particular to a super-lubricating composite material for bionic cartilage and subchondral bone and a preparation method of the super-lubricating composite material. The super-lubricating composite material is formed by compounding a hydrogel precursor, a polyvinyl alcohol aqueous solution and a functional filler under an alkaline condition, the hydrogel precursor is prepared by carrying out a reaction on sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and m-aminophenylboronic acid in deionized water, and the hydrogel precursor is prepared by carrying out a reaction on the sodium alginate, the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and the m-aminophenylboronic acid; the composite material prepared by the invention has excellent mechanical properties, elastic modulus of bionic cartilage and extremely low friction coefficient, and also has excellent lubricating stability and self-repairing function, so that dynamic repairing and lubrication maintenance of a damaged interface are realized; the material is particularly suitable for a bionic cartilage layer in femoral head necrosis area replacement, friction between an implant and original cartilage can be effectively reduced, cartilage repair is promoted, and the joint function is improved.

The invention discloses an RIP3-mediated osteoarthritis animal model as well as a construction method and application thereof. The construction method comprises the following steps: delivering an effective dose of a genetic tool (such as an adenovirus vector) capable of expressing RIP3 protein into a knee joint subchondral bone area of an experimental animal, and realizing continuous overexpression of RIP3 through regular operation, so as to induce pathological characteristics highly similar to human osteoarthritis. The model stably shows typical OA phenotypes such as limb pain sensitization, subchondral bone loss and bone microstructure degeneration, osteoclast number increase and overlying cartilage degeneration. The invention reveals that the natural process of occurrence and development of the osteoarthritis can be directly simulated through specific overexpression of the RIP3 in the subchondral bone for the first time, and a brand-new and valuable tool is provided for researching the pathogenesis of the osteoarthritis and screening and evaluating medicines and therapies for treating the osteoarthritis.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

The invention relates to the technical field of biomedical materials, in particular to a double-network hydrogel material for bionic cartilage and subchondral bone and a preparation method of the double-network hydrogel material. According to the double-network hydrogel material for the bionic cartilage and the subchondral bone, polyvinyl alcohol is adopted as a first network skeleton, and a high-toughness hydrogel structure is formed through a circulating freezing and thawing method; sulfobetaine methacrylate and a calcium chloride solution are introduced as a second network cross-linking system, so that the material is endowed with excellent hydrophilic lubrication performance; furthermore, liposome microspheres are compounded to form a self-renewal liposome boundary layer, so that the friction coefficient is remarkably reduced; the material has high strength, high toughness, low friction, self-lubrication and biocompatibility, is suitable for precise repair and functional reconstruction of osteochondral defects such as femoral head necrosis and the like, and has a wide clinical application prospect.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused 2024 / 064830 by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the metatarsal phalangeal joint or other joint of the foot or ankle, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.WO

The invention discloses a double-layer piezoelectric hydrogel stent for temporal-mandibular joint injury repair and a preparation method and application thereof.The piezoelectric hydrogel stent comprises an upper stent layer for cartilage repair and a lower stent layer for subchondral bone repair, and the upper layer and the lower layer both comprise a colloid component and a piezoelectric component; wherein the mass percent concentration of piezoelectric components in the upper layer of the piezoelectric hydrogel stent is lower than that of piezoelectric components in the lower layer of the piezoelectric hydrogel stent. The mechanical property and the gradient piezoelectric property of the double-layer piezoelectric hydrogel stent are matched with tissue regeneration at the temporomandibular joint, and cartilage-bone synergistic regeneration at the temporomandibular joint can be accurately regulated and controlled. The novel bionic hydrogel stent disclosed by the invention can remarkably improve temporal-mandibular joint injury and has a wide clinical application prospect.

This invention discloses a neutralizing nanobody targeting soluble interleukin-17 receptor D (sIL-17RD), its preparation method, and its applications. The heavy chain variable region (VHH) of this nanobody contains a heavy chain complementarity-determining region 3 (CDR-H3) that specifically binds to sIL-17RD, with a length of 10 amino acids and containing 2–6 lysine residues. This invention provides four nanobodies: CQ5, CQ13, CQ11, and CQ6, corresponding to well-defined CDR-H1, CDR-H2, and CDR-H3 sequence combinations. This nanobody can specifically bind to and neutralize the biological activity of sIL-17RD, reducing chondrocyte death, promoting proliferation, and maintaining extracellular matrix homeostasis by inhibiting the P2rx7-NLRP3 inflammasome-mediated chondrocyte pyroptosis pathway. This invention also protects the nucleic acid encoding this nanobody, the recombinant expression vector, the host cell, the pharmaceutical composition, and the preparation method. The nanobodies described can be used to prepare drugs for the prevention and treatment of inflammatory osteoarthritis and other osteoarthritis. They can inhibit synovitis, reverse cartilage and subchondral bone degeneration, and relieve pain, showing promising application prospects.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

An adaptive porous femoral head necrosis support rod comprises a head, a body, a temperature-sensitive bone conduction layer and a tail; the head, the body and the tail are coaxially connected in sequence, and the temperature-sensitive bone conduction layer covers the surface of the body; the shape of the head matches the cavity surface left after the removal of dead bone, and the head and the body are both porous structures; the tail is provided with a thread, and the thread groove is provided with a porous structure. The porous personalized adaptive femoral head necrosis support rod has strong personalized adaptation. The shape of the head matches the cavity surface left after the removal of dead bone in the patient's marrow core, can maximize the contact area of the head and the patient's subchondral bone, improve the ability of the support rod to resist pressure and support the subchondral bone, and effectively prevent collapse.

The invention provides application of a composition in treatment of knee osteoarthritis. According to the invention, two rat knee osteoarthritis models (the former is called as an ACLT-induced KOA model for short, and the latter is called as a papain-induced KOA model for short) are established by respectively utilizing methods of combining anterior cruciate ligament transection (ACLT) with running and injecting papain composite L-cysteine into an articular cavity; the composition provided by the invention is used for treating two rat KOA models. Results show that the composition can improve knee joint swelling of two KOA rats, increase the pain threshold, improve the abnormal gait state, relieve cartilage injury, inhibit ACLT-induced abnormal change of a KOA rat knee joint subchondral bone microstructure and papain-induced PGE2 content in KOA rat synovial tissue, and can be used for preparing a medicine for treating osteoporosis. The level of inflammatory factors in serum and joint lavage fluid of the two KOA rats is reduced, and the discovery shows that the KOA has important application in treatment of knee osteoarthritis.

The invention provides a 3D printing double-layer gradient composite scaffold and a preparation method and application thereof, the 3D printing double-layer gradient composite scaffold is prepared by adopting a multi-nozzle collaborative printing technology of a 3D printing system, and the 3D printing double-layer gradient composite scaffold comprises a cartilage repair layer and a subchondral bone repair layer. Wherein the cartilage repair layer and the subchondral bone repair layer are respectively prepared by cooperatively printing a thermoplastic polymer material and a photocurable natural polymer material mixed with cells and double drug-loaded nanoparticles through double nozzles of a 3D printing system. The distance between the printed microwires of the cartilage repair layer is smaller than that between the printed microwires of the subchondral bone repair layer. The double-layer gradient composite scaffold is integrally prepared by using a multi-nozzle collaborative printing technology of a 3D printing system, has good mechanical strength and biocompatibility, and can realize integrated repair of osteochondral defects.

The invention discloses a double-layer piezoelectric hydrogel scaffold for realizing articular cartilage injury repair through ultrasonic regulation, the piezoelectric hydrogel scaffold comprises a scaffold upper layer for cartilage repair and a scaffold lower layer for subchondral bone repair, the upper layer and the lower layer both comprise a colloid component and a piezoelectric component, wherein the mass percent concentration of piezoelectric components in the upper layer of the piezoelectric hydrogel stent is lower than that of piezoelectric components in the lower layer of the piezoelectric hydrogel stent. The mechanical property and gradient piezoelectric property of the double-layer piezoelectric hydrogel scaffold are matched with articular bone cartilage tissue regeneration, and cartilage-bone synergistic regeneration can be accurately regulated and controlled. The novel bionic hydrogel scaffold can significantly improve articular cartilage injury, and has a wide clinical application prospect.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the humeroradial joint or other joint of the elbow, wrist, or hand, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The invention discloses an oleanolic acid cubic liquid crystal nanoparticle temperature-sensitive gel preparation and application thereof. The preparation is prepared by dispersing oleanolic acid cubic liquid crystal nanoparticles in a temperature-sensitive gel matrix, the oleanolic acid cubic liquid crystal nanoparticles are prepared from oleanolic acid, phytantriol and a stabilizer, and the temperature-sensitive gel matrix is composed of poloxamer F127 and poloxamer F68. The preparation disclosed by the invention has good temperature-sensitive characteristic, slow release performance and joint lubrication function, and can effectively prolong the residence time of oleanolic acid in a joint cavity and improve the bioavailability of oleanolic acid. Experimental results show that the preparation can effectively relieve pain and depression-like behaviors related to knee osteoarthritis, inhibit cartilage degradation and subchondral osteosclerosis, regulate inflammatory factors and oxidative stress indexes and systematically improve the functions of hippocampus through a brain-bone axis, and has important clinical application value.

The invention discloses ROS (reactive oxygen species) response type liposome hydrogel as well as a preparation method and application thereof, and the preparation method comprises the following steps: sequentially preparing drug-loaded liposome, phenylboronic acid modified hyaluronic acid and ROS response type hydrogel, and mixing the drug-loaded liposome and the ROS response type hydrogel to obtain the ROS response type liposome hydrogel. According to the preparation method disclosed by the invention, through surface modification of the cartilage affinity peptide and ROS responsive hydrogel encapsulation, the targeting capability and the retention capability of the medicines resveratrol and chondroitin sulfate are successfully improved, and the purpose of responsive release of the medicines is achieved. According to the ROS response type liposome hydrogel, accumulation of ROS in cells can be reduced, and the inflammatory mediator and matrix degrading enzyme level can be lowered; meanwhile, cartilage matrix degradation and subchondral bone change can be inhibited, development of osteoarthritis is delayed, and the novel delivery system for osteoarthritis treatment has huge potential.

The utility model discloses a kind of minimally invasive implant artificial partial surface femoral head joint prosthesis, comprising: umbrella handle;Cylinder is sleeved on umbrella handle and is movably connected with it;First support prosthesis is sleeved on umbrella handle and is located above cylinder, with multiple first umbrella leaves in fan shape;Second support prosthesis is sleeved on umbrella handle and is located above cylinder, with multiple second umbrella leaves in fan shape being set in multiple first umbrella leaves dislocation;Wherein, multiple first umbrella leaves and multiple second umbrella leaves can be opened to complete umbrella-shaped joint prosthesis by relative umbrella handle under the impetus of cylinder.The utility model head joint prosthesis, can replace cartilage and subchondral bone of femoral head necrosis area, form good mechanical support, relieve patient discomfort, weight bearing with prosthesis, reduce femoral head endosteal bone weight bearing, slow down even reverse the progress of femoral head necrosis, can reach minimally invasive artificial joint partial replacement effect, reduce surgical trauma.

The application provides a Chinese medicine composition containing Notopterygium incisum and Lonicera japonica in preparation and treatment of rheumatoid arthritis, and belongs to the technical field of medicine; scientific research shows that the Chinese medicine composition containing Notopterygium incisum and Lonicera japonica can reduce joint cartilage destruction, subchondral bone destruction caused by rheumatoid arthritis, promote osteoblast differentiation and mineralization, reduce joint swelling, synovial hyperplasia, and inflammatory cell infiltration of joints caused by rheumatoid arthritis, has the effect of treating rheumatoid arthritis, and can be developed into a medicine for treating rheumatoid arthritis.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

The invention provides a bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration and a preparation method of the bionic magnesium alloy material, and relates to the technical field of biomedical materials. The bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration comprises a porous magnesium alloy stent, and bionic hydrogel HAMA-RGD (at) BMM-KGN and HAMA-RGD (at) BMM-Q which are cured in pores of the porous magnesium alloy stent in a layered manner; the HAMA-RGD (at) BMM-KGN is a bionic hydrogel formed by dispersing the BMM loaded with the KGN into a HAMA-RGD solution, and the BMM loaded with the KGN is coated with the HAMA-RGD. The HAMA-RGD (at) BMM-Q is a bionic hydrogel which is formed by dispersing BMM loaded with quercetin Q in an HAMA-RGD solution. The bionic magnesium alloy material for inducing bone-cartilage synchronous regeneration can provide stable three-dimensional mechanical support macroscopically, reconstruct a spatial gradient structure of cartilage and subchondral bone microscopically, and realize'bone-cartilage 'synchronous and partitioned regeneration.

The invention belongs to the technical field of bone tissue engineering and regenerative medicine, and particularly discloses a piezoelectric-photo-thermal dual-mode composite stent for articular cartilage repair and a preparation method of the piezoelectric-photo-thermal dual-mode composite stent. The preparation method comprises the three steps of MXene preparation, electrostatic spinning and paper folding structure construction, poly-L-lactic acid and MXene nanosheets are compounded to prepare a fiber membrane, and then the fiber membrane is subjected to hot pressing through a corrugated mold to form the three-dimensional stent with the periodic creased structure. The scaffold has piezoelectric property and photo-thermal property at the same time, promotes cartilage differentiation through the piezoelectric effect, inhibits subchondral bone angiogenesis through the photo-thermal effect, maintains a low-oxygen microenvironment, realizes integral repair of cartilage-subchondral bone functional units, can be used for treatment of cartilage defects, osteochondral defects and osteoarthritis, and has a wide application prospect. The problem that the cartilage steady state is damaged due to the fact that a traditional piezoelectric material promotes bone vascularization is solved.

This invention belongs to the biomedical field, specifically relating to the use of BSP-MA / HA-NB hydrogel in the preparation of drugs for treating bone defects, cartilage damage, or degenerative diseases of articular cartilage. This invention introduces HA-NB (hyaluronic acid modified with o-nitrosobenzaldehyde) into BSP-MA (methacrylated Bletilla striata polysaccharide) to prepare BSP-MA / HA-NB hydrogel, which exhibits a significant synergistic effect in promoting cartilage and subchondral bone regeneration. Simultaneously, it can reduce inflammatory responses and promote tissue repair by inhibiting the activation of M1 macrophages and promoting the increase of M2 macrophages. It can be used to treat cartilage damage and / or degenerative diseases of articular cartilage, and has broad application prospects.