100 results about "Subchondral bone" patented technology

The invention relates to an in-situ osteochondral regeneration and repair scaffold and a preparation method thereof, the in-situ osteochondral regeneration and repair scaffold prepared by the method comprises a cartilage layer, a connecting layer and a subchondral bone layer, the porosity of the cartilage layer is not less than 85%, the Young modulus is 6-13KPa, and the aperture is 130-140 [mu] m; the porosity of the connecting layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 50-120 [mu] m; the porosity of the subchondral bone layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 300-400 microns. The in-situ osteochondral regeneration and repair scaffold prepared by the method has good biocompatibility, is beneficial to attachment, migration and proliferation of cartilage cells and bone marrow mesenchymal stem cells, does not cause immunological rejection, provides a better scaffold environment for repair after osteochondral implantation, and has a good application prospect in the field of in-situ osteochondral regeneration and repair, and experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good application prospect in the field of in-situ osteochondral regeneration and repair. The in-situ osteochondral regeneration and repair scaffold does not affect cell migration; animal experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good repair effect on rat osteochondral defects.

An ankle joint replacement prosthesis comprises a tibia prosthesis assembly and a talus prosthesis, the tibia prosthesis assembly comprises a tibia prosthesis body and a cushion block, the tibia prosthesis body and the cushion block are in an arc shape with the middle bent upwards on the sagittal plane, the cushion block is arranged at the bottom of the tibia prosthesis body in a sleeving mode, and the surface of the upper portion of the tibia prosthesis body is a tibia joint face. The surface of the lower portion of the cushion block is a first articular surface, the surface of the upper portion of the talus prosthesis is a second articular surface, the second articular surface is in an arc shape with the middle bent upwards on a sagittal plane, the surface of the lower portion of the talus prosthesis is a talus joint surface, and the first articular surface is matched with the second articular surface. When the prosthesis is implanted, only the articular cartilage and the subchondral bone need to be treated at the far end of the tibia, so that the bone at the far end of the tibia is reserved to the maximum extent, the anatomical integrity of the bone is guaranteed, the stability of the prosthesis is enhanced, the service life is prolonged, and the clinical operation effect is improved.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention belongs to the technical field of biological medicines, and particularly discloses application of ORM1 protein or VIM knockout adenovirus in preparation of a medicine for treating temporomandibular joint osteoarthritis (TMJOA). The invention proves that the expression of ORM1 is reduced in synovial fluid of a TMJOA patient and a unilateral anterior tooth reverse inhale model rat, and the degradation of cartilage matrix of the UAC rat can be remarkably inhibited and the progress of TMJOA can be relieved by locally injecting ORM1 protein. On the mechanism level, ORM1 is combined with waveform protein VIM to inhibit an MAPK channel, so that expression of MMP13 and MMP3 is reduced, cartilage matrix degradation is reduced, and cartilage steady state is maintained. In addition, the invention also finds that after the VIM of the rat is knocked out by using the adeno-associated virus, the progress of TMJOA, including cartilage degeneration and subchondral bone destruction, is also obviously improved. Therefore, the invention provides a new therapeutic target and a new therapeutic strategy for the TMJOA.

The invention relates to the technical field of medical image auxiliary diagnosis, and provides an intervertebral joint osteoarthritis image feature evaluation method based on deep learning, which can synchronously identify five types of FJOA image features such as joint space stenosis, osteophyte, hypertrophy, subchondral bone erosion and subchondral cyst, and improves the comprehensiveness and efficiency of evaluation. The nnU-Net model is adopted to perform high-precision segmentation on an intervertebral joint region, so that the positioning precision is effectively improved; by introducing a shared feature extraction network based on ResNet-18 and five parallel classification sub-networks, joint modeling of multi-scale semantic information is realized, and the recognition accuracy and generalization ability of the model are improved; according to the method, a Grad-CAM technology is combined to generate an activation thermodynamic diagram, a model interpretation basis is superposed to an original image, visual display of an evaluation result is realized, interpretability and clinical applicability of the model are enhanced, and FJOA image features in axial lumbar vertebra CT images from two central data sets can be quantitatively evaluated so as to comprehensively quantify individual FJOA image features.

The invention belongs to the technical field of hydrogel preparation, and relates to a cartilage-imitating multifunctional drug release hydrogel material and a preparation method thereof. According to the invention, biocompatible high polymer materials such as polyvinyl alcohol, chitosan, grape seed protein, acyl chloride, sulphobetaine methacrylate and lecithin are used as raw materials, a cartilage structure is simulated to prepare the multilayer hydrogel, gelatin and plant polyphenol are mixed as an adhesive to improve the binding capacity with subosseous bones, and a drug is loaded to improve the biological activity. The preparation method of the hydrogel material comprises the five steps of preparation of high-strength anti-swelling precursor hydrogel, introduction of double bonds through surface modification, introduction of a poly SBMA molecular brush through interfacial polymerization, drug loading and preparation of an adhesion layer. The hydrogel has excellent anti-swelling ability, lubricating property, anti-pollution ability, free radical scavenging ability, self-adhesion characteristic, drug controlled release ability and biocompatibility, and has important significance for widening the application of the hydrogel in the field of artificial cartilage.

Disclosed herein are methods of treatment of pain associated with osteoarthritis, pain associated with osteonecrosis, and pain associated with one or more subchondral insufficiency fractures; methods of improving physical function in a human subject with osteoarthritis, osteonecrosis, or one or more subchondral insufficiency fractures; and methods of treating osteoarthritis, or delaying or slowing the progression of osteoarthritis, or relieving a symptom of osteoarthritis, in humans with these diseases, comprising administering a therapeutically effective amount of a composition comprising a glycosylated p75NTR neurotrophin binding protein (NBP)-Fc fusion protein.

The invention proves that the isosakuranetin can inhibit the generation of osteoclasts and the functions of the osteoclasts in vitro, protect the bone microstructure of subchondral bones of an OA (osteoarthritis) mouse, relieve the degeneration of articular cartilage of the OA mouse, inhibit the generation of abnormal blood vessels in subchondral marrow cavities in the progress of the OA, and inhibit the osteoclasts in the osteoclasts. The invention proves that the isosakuranetin has a treatment effect on the osteoarthritis for the first time, and discloses an action mechanism of the isosakuranetin.

The invention provides a controllable degradation magnesium-based bionic biphasic stent as well as a preparation method and application thereof. The stent comprises a porous magnesium stent, hydrogel and a ceramic coating, the porous magnesium scaffold comprises a cartilage phase scaffold and a subchondral bone phase scaffold which are arranged from top to bottom; hydrogel is arranged on pores and the outer surface of the cartilage phase scaffold, and a ceramic coating is arranged on the surface of the subchondral bone phase scaffold. From the bionic angle, the three-dimensional communicated porous magnesium stent with gradient porosity is designed by simulating the structure, mechanics and other characteristics of joint tissues (upper cartilage and lower subchondral bone); meanwhile, according to the characteristics of joint tissue components, biological properties and the like, hydrogel (an upper cartilage phase) is filled in the stent, and a bioactive ceramic coating (a lower subchondral bone phase) is prepared on the surface of the stent, so that the degradation rate of the biphasic magnesium stent is regulated and controlled, and the cytotoxicity is reduced; meanwhile, the biocompatibility and the biological activity are improved, and endogenous cartilage-subchondral bone integrated repair is realized.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the femorotibial joint, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The utility model belongs to the technical field of medical instruments, and relates to a joint implant for the orthopedics department. The joint implant is used for joint bone cartilage injury and defect repair and comprises a joint implant base body with a porous titanium structure and a top solid self-lubricating film. The joint implant base includes a top region, an upper region, and a lower region. The top area is matched with the outer contour face of the human body bone and is of a dense mirror face structure, the surface of the top area is plated with a solid self-lubricating film, abrasion to the opposite-side cartilage can be reduced to a large extent, and degeneration of the opposite-side cartilage is reduced. The side faces of the top area and the upper area of the joint implant base body make contact with subchondral bones and cancellous bones of the human body, and the lower area of the joint implant base body makes contact with the cancellous bones of the human body. The aperture and porosity of the joint implant base body extend from the upper area to the lower area, are increased in a gradient manner, are matched with the mechanical properties of human bones, and are beneficial to initial stability, early-stage rapid bone ingrowth and long-term stability.

The present invention relates generally to minimally invasive, cost-effective, adaptable methods, systems, and devices used to repair anatomical joint conditions. The repair may be necessitated by trauma, disease or other conditions. The anatomical joint may specifically include mammalian joints such as the knee, shoulder, elbow, wrist, finger, hip, spine, toe and ankle, for example. The methods, systems, and devices disclosed herein include leveraging the significant (and often unappreciated) role the subchondral bone plays in the health status of the afflicted anatomical joint.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

The present invention demonstrates that isosakurabin can inhibit the formation and function of osteoclasts in vitro, protect the bone microstructure of the subchondral bone in mice with osteoarthritis (OA), reduce articular cartilage degeneration in OA mice, inhibit the formation of abnormal blood vessels in the subchondral bone marrow cavity during OA progression, and alleviate joint pain induced by abnormal osteoclast activation in OA mice. This invention demonstrates for the first time that isosakurabin has a therapeutic effect on osteoarthritis and reveals its mechanism of action.

The present application relates to the prevention and treatment of osteoarticular diseases, in particular osteoarthritis (OA), by inhibition of acetylcholinesterase (AChE). Specifically, this application provides a disease progression-modifying drug for the treatment of osteoarthritis, i.e., an acetylcholinesterase inhibitor, in particular a dimeric acetylcholinesterase inhibitor. The dimer targets both the enzymatic triad and non-enzymatic peripheral sites of acetylcholinesterase, thereby enhancing protection of articular cartilage and subchondral bone.

A method for modeling temporomandibular joint osteoarthritis, 6-week-old rats are selected, the rats are confirmed to have entered an anesthetized state before surgery, the rat's mandibular incisors are first checked and confirmed to be normal, the first arm of the device is sleeved on one side of the mandibular incisor, and the palatal side of the maxillary incisor is made to touch the slope surface of the second arm of the device, the device is checked and confirmed to not affect the rat's mandibular movement and occlusion, not significantly raise the bite, have no early contact, and the upper and lower jaw relationship is relatively stable; the device and the mandibular incisor sleeved with the device are fixed; then the rat is put back into the cage, and soft food or powdered feed is given after waking up; the device is removed after being fed for 8 weeks or more. It is verified that the condylar process of the rat changes significantly, the overall condylar process is significantly reduced, the subchondral bone is destroyed, and the expression of the cartilage matrix is also reduced. Thus, a TMJOA rat model exhibiting mandibular retrusion is formed.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The invention relates to the technical field of biomedical materials, in particular to a super-lubricating composite material for bionic cartilage and subchondral bone and a preparation method of the super-lubricating composite material. The super-lubricating composite material is formed by compounding a hydrogel precursor, a polyvinyl alcohol aqueous solution and a functional filler under an alkaline condition, the hydrogel precursor is prepared by carrying out a reaction on sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and m-aminophenylboronic acid in deionized water, and the hydrogel precursor is prepared by carrying out a reaction on the sodium alginate, the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and the m-aminophenylboronic acid; the composite material prepared by the invention has excellent mechanical properties, elastic modulus of bionic cartilage and extremely low friction coefficient, and also has excellent lubricating stability and self-repairing function, so that dynamic repairing and lubrication maintenance of a damaged interface are realized; the material is particularly suitable for a bionic cartilage layer in femoral head necrosis area replacement, friction between an implant and original cartilage can be effectively reduced, cartilage repair is promoted, and the joint function is improved.

The invention discloses an RIP3-mediated osteoarthritis animal model as well as a construction method and application thereof. The construction method comprises the following steps: delivering an effective dose of a genetic tool (such as an adenovirus vector) capable of expressing RIP3 protein into a knee joint subchondral bone area of an experimental animal, and realizing continuous overexpression of RIP3 through regular operation, so as to induce pathological characteristics highly similar to human osteoarthritis. The model stably shows typical OA phenotypes such as limb pain sensitization, subchondral bone loss and bone microstructure degeneration, osteoclast number increase and overlying cartilage degeneration. The invention reveals that the natural process of occurrence and development of the osteoarthritis can be directly simulated through specific overexpression of the RIP3 in the subchondral bone for the first time, and a brand-new and valuable tool is provided for researching the pathogenesis of the osteoarthritis and screening and evaluating medicines and therapies for treating the osteoarthritis.

The utility model provides a composite cartilage scaffold. The composite cartilage scaffold comprises a cartilage scaffold layer, a calcification scaffold layer and a subchondral bone scaffold layer which are sequentially stacked and arranged from top to bottom, a plurality of first immersion channels communicated with each other are arranged in the cartilage stent layer and the calcification stent layer, a plurality of first immersion holes are formed in the top of the cartilage stent layer, a plurality of second immersion holes are formed in the side wall of the calcification stent layer, and the first immersion holes and the second immersion holes are communicated with the first immersion channels. Before an operation, the cartilage scaffold layer and the calcification scaffold layer are soaked with cartilage cells, and the cartilage cells are rapidly immersed into the first immersion channel through the first immersion hole and the second immersion hole, so that the cartilage scaffold layer and the calcification scaffold layer can absorb more sufficient cartilage cells, and the cartilage cell content of the scaffold is increased; the secretion of cartilage extracellular matrix is better promoted, the extension of cartilage cells and the coupling between the cartilage cells and in-situ cartilage are facilitated, and the repair of cartilage injury is further promoted.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The application discloses an enzyme response self-assembly polypeptide modified methacrylating polymer and a preparation method and application thereof, and the enzyme response self-assembly polypeptide modified methacrylating polymer has a structure of formula 1. The application converts an endogenous tissue-specific marker alkaline phosphatase concentration gradient into a dynamic gradient evolution of the mechanical properties of a scaffold. By introducing an enzyme response self-assembly polypeptide into a methacrylating polymer structure, the scaffold is subjected to molecular level dynamic self-assembly under the catalysis of phosphatase, and a continuous mechanical gradient is formed from a cartilage region to a subchondral bone region. The process does not require external intervention and is completely regulated by a local microenvironment, thereby real-time adapting to the dynamic requirements of 'early proliferation-late differentiation' of bone cartilage repair. Compared with the prior art, the application not only breaks through the mismatch between a static gradient and a dynamic microenvironment, but also precisely regulates mechanical signals at a molecular scale, and significantly improves the spatial specificity and time synchronism of stem cell differentiation.

The invention relates to an in-situ lubrication 3D printing Janus bone cartilage repair scaffold and a preparation method thereof.The scaffold is prepared through 3D printing and comprises a subchondral bone layer and a cartilage layer, the subchondral bone layer is prepared from GelMA and NAGA loaded nHA, and bone healing is promoted; the cartilage layer is provided with zwitterionic monomers, and in-situ lubrication is achieved. In-situ lubrication can be realized through lubrication modification of the cartilage layer scaffold structure, and bone healing is promoted by loading hydroxyapatite on the subchondral bone layer scaffold.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

The present disclosure relates to formulations for the treatment of osteoarthritis. Disclosed are formulations for treating osteoarthritis by altering a change in shape of articular subchondral bone. The use includes assessing the bone shape of the patient's joint, injecting the patient with a peptide of SEQ ID NO: 1 or applying other therapeutic interventions that reduce changes in the shape of the articular subchondral bone, and thereby assessing the bone shape of the patient's joint.

The invention relates to the technical field of biomedical materials, in particular to a double-network hydrogel material for bionic cartilage and subchondral bone and a preparation method of the double-network hydrogel material. According to the double-network hydrogel material for the bionic cartilage and the subchondral bone, polyvinyl alcohol is adopted as a first network skeleton, and a high-toughness hydrogel structure is formed through a circulating freezing and thawing method; sulfobetaine methacrylate and a calcium chloride solution are introduced as a second network cross-linking system, so that the material is endowed with excellent hydrophilic lubrication performance; furthermore, liposome microspheres are compounded to form a self-renewal liposome boundary layer, so that the friction coefficient is remarkably reduced; the material has high strength, high toughness, low friction, self-lubrication and biocompatibility, is suitable for precise repair and functional reconstruction of osteochondral defects such as femoral head necrosis and the like, and has a wide clinical application prospect.

The invention relates to an osteochondral scaffold with biological energy activity and a preparation method of the osteochondral scaffold, and belongs to the technical field of biological materials and tissue engineering. The method comprises the following steps: carrying out first chemical reaction on an intermediate of tricarboxylic acid cycle and trihydric alcohol to obtain a bio-energy material prepolymer; performing a second chemical reaction on the bio-energy material prepolymer and a cross-linking agent, and adding an osteogenesis promoting agent into a system of the second chemical reaction to obtain composite slurry; regulating and controlling the weight of the osteogenesis promoting agents in the composite slurry to obtain three biological energy material inks containing the osteogenesis promoting agents with different weights; carrying out gradient type 3D printing on the three kinds of biological energy material ink containing the osteogenesis promoting agents with different weights so as to respectively simulate a cartilage layer, a transition layer and a subchondral bone layer in the articular cartilage, and carrying out a third chemical reaction after 3D printing, so as to obtain the target osteochondral scaffold. The osteochondral scaffold provided by the invention has biological energy activity, and realizes regeneration of transparent cartilage and reconstruction of an osteochondral complex interface.

The invention discloses an osteochondral repair scaffold and a preparation method thereof.The osteochondral repair scaffold comprises a subchondral bone layer, a calcified cartilage layer and a cartilage layer which are sequentially connected, and the calcified cartilage layer comprises an upper calcified cartilage layer, a middle calcified cartilage layer and a lower calcified cartilage layer; according to the osteochondral repair scaffold and the preparation method thereof, the layered structure is adopted to simulate the physiological cartilage of the human body, and through the layering and gradient porosity design of the calcified cartilage layer, the flexibility of the scaffold is improved; the use performance of the scaffold is improved, and it can be ensured that the scaffold can rapidly recruit autoblood of a patient after being implanted into an osteochondral defect part, so that mesenchymal stem cells are guided to be differentiated into cartilage cells in a cartilage layer, and cartilage tissue generation is promoted.

Disclosed herein are functionalized branched poly-lysine compounds and uses thereof, as well as delivery systems in which polymeric nanocarriers are functionalized with branched poly-lysine compounds. Also provided herein are uses of the functionalized branched poly-lysine compounds and the delivery systems for treating joint diseases such as osteoarthritis, and in delivering pharmaceutically active compounds to cartilage and subchondral bone.