34 results about "Bone epiphysis" patented technology

The invention provides a controllable degradation magnesium-based bionic biphasic stent as well as a preparation method and application thereof. The stent comprises a porous magnesium stent, hydrogel and a ceramic coating, the porous magnesium scaffold comprises a cartilage phase scaffold and a subchondral bone phase scaffold which are arranged from top to bottom; hydrogel is arranged on pores and the outer surface of the cartilage phase scaffold, and a ceramic coating is arranged on the surface of the subchondral bone phase scaffold. From the bionic angle, the three-dimensional communicated porous magnesium stent with gradient porosity is designed by simulating the structure, mechanics and other characteristics of joint tissues (upper cartilage and lower subchondral bone); meanwhile, according to the characteristics of joint tissue components, biological properties and the like, hydrogel (an upper cartilage phase) is filled in the stent, and a bioactive ceramic coating (a lower subchondral bone phase) is prepared on the surface of the stent, so that the degradation rate of the biphasic magnesium stent is regulated and controlled, and the cytotoxicity is reduced; meanwhile, the biocompatibility and the biological activity are improved, and endogenous cartilage-subchondral bone integrated repair is realized.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the femorotibial joint, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The present invention relates generally to minimally invasive, cost-effective, adaptable methods, systems, and devices used to repair anatomical joint conditions. The repair may be necessitated by trauma, disease or other conditions. The anatomical joint may specifically include mammalian joints such as the knee, shoulder, elbow, wrist, finger, hip, spine, toe and ankle, for example. The methods, systems, and devices disclosed herein include leveraging the significant (and often unappreciated) role the subchondral bone plays in the health status of the afflicted anatomical joint.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

The present invention demonstrates that isosakurabin can inhibit the formation and function of osteoclasts in vitro, protect the bone microstructure of the subchondral bone in mice with osteoarthritis (OA), reduce articular cartilage degeneration in OA mice, inhibit the formation of abnormal blood vessels in the subchondral bone marrow cavity during OA progression, and alleviate joint pain induced by abnormal osteoclast activation in OA mice. This invention demonstrates for the first time that isosakurabin has a therapeutic effect on osteoarthritis and reveals its mechanism of action.

A method for modeling temporomandibular joint osteoarthritis, 6-week-old rats are selected, the rats are confirmed to have entered an anesthetized state before surgery, the rat's mandibular incisors are first checked and confirmed to be normal, the first arm of the device is sleeved on one side of the mandibular incisor, and the palatal side of the maxillary incisor is made to touch the slope surface of the second arm of the device, the device is checked and confirmed to not affect the rat's mandibular movement and occlusion, not significantly raise the bite, have no early contact, and the upper and lower jaw relationship is relatively stable; the device and the mandibular incisor sleeved with the device are fixed; then the rat is put back into the cage, and soft food or powdered feed is given after waking up; the device is removed after being fed for 8 weeks or more. It is verified that the condylar process of the rat changes significantly, the overall condylar process is significantly reduced, the subchondral bone is destroyed, and the expression of the cartilage matrix is also reduced. Thus, a TMJOA rat model exhibiting mandibular retrusion is formed.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The invention discloses an RIP3-mediated osteoarthritis animal model as well as a construction method and application thereof. The construction method comprises the following steps: delivering an effective dose of a genetic tool (such as an adenovirus vector) capable of expressing RIP3 protein into a knee joint subchondral bone area of an experimental animal, and realizing continuous overexpression of RIP3 through regular operation, so as to induce pathological characteristics highly similar to human osteoarthritis. The model stably shows typical OA phenotypes such as limb pain sensitization, subchondral bone loss and bone microstructure degeneration, osteoclast number increase and overlying cartilage degeneration. The invention reveals that the natural process of occurrence and development of the osteoarthritis can be directly simulated through specific overexpression of the RIP3 in the subchondral bone for the first time, and a brand-new and valuable tool is provided for researching the pathogenesis of the osteoarthritis and screening and evaluating medicines and therapies for treating the osteoarthritis.

The invention relates to an in-situ lubrication 3D printing Janus bone cartilage repair scaffold and a preparation method thereof.The scaffold is prepared through 3D printing and comprises a subchondral bone layer and a cartilage layer, the subchondral bone layer is prepared from GelMA and NAGA loaded nHA, and bone healing is promoted; the cartilage layer is provided with zwitterionic monomers, and in-situ lubrication is achieved. In-situ lubrication can be realized through lubrication modification of the cartilage layer scaffold structure, and bone healing is promoted by loading hydroxyapatite on the subchondral bone layer scaffold.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused 2024 / 064830 by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the metatarsal phalangeal joint or other joint of the foot or ankle, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.WO

There is disclosed a defect sizer tool for selection of an appropriately sized pre-cut core for a cryopreserved osteochondral allograft (COCA) transplantation procedure. In an embodiment, the tool includes an elongated connector that provides a handle portion. A ring measurement guide at one end, with a given diameter, and alignable with a cartilage defect to provide an estimated measurement of a diameter. There is disclosed an instrument kit with a pin, a pin guide, and a reamer configured to slidingly receive the pin, blades at one end, and a rotational drive connector at the other end. In an embodiment, a method for a COCA transplantation procedure includes providing an instrument kit, using the pin guide to place the pin in the subchondral bone, and operating the reamer to score surrounding cartilage prior to the reamer bit portion reaming the subchondral bone to the desired depth. Other embodiments are also disclosed.

An adaptive porous femoral head necrosis support rod comprises a head, a body, a temperature-sensitive bone conduction layer and a tail; the head, the body and the tail are coaxially connected in sequence, and the temperature-sensitive bone conduction layer covers the surface of the body; the shape of the head matches the cavity surface left after the removal of dead bone, and the head and the body are both porous structures; the tail is provided with a thread, and the thread groove is provided with a porous structure. The porous personalized adaptive femoral head necrosis support rod has strong personalized adaptation. The shape of the head matches the cavity surface left after the removal of dead bone in the patient's marrow core, can maximize the contact area of the head and the patient's subchondral bone, improve the ability of the support rod to resist pressure and support the subchondral bone, and effectively prevent collapse.

The invention discloses a double-layer piezoelectric hydrogel scaffold for realizing articular cartilage injury repair through ultrasonic regulation, the piezoelectric hydrogel scaffold comprises a scaffold upper layer for cartilage repair and a scaffold lower layer for subchondral bone repair, the upper layer and the lower layer both comprise a colloid component and a piezoelectric component, wherein the mass percent concentration of piezoelectric components in the upper layer of the piezoelectric hydrogel stent is lower than that of piezoelectric components in the lower layer of the piezoelectric hydrogel stent. The mechanical property and gradient piezoelectric property of the double-layer piezoelectric hydrogel scaffold are matched with articular bone cartilage tissue regeneration, and cartilage-bone synergistic regeneration can be accurately regulated and controlled. The novel bionic hydrogel scaffold can significantly improve articular cartilage injury, and has a wide clinical application prospect.

Bone and joint surface reconstruction methods are disclosed for the therapeutic amelioration of joint defects caused by various conditions, injuries, and diseases to help eliminate or reduce pain and return the joint to its proper bio-mechanical function without the need to replace fully or partially the anatomical joint. The joint may specifically include mammalian joints such as the humeroradial joint or other joint of the elbow, wrist, or hand, for example. The methods disclosed herein leverage the significant role the subchondral bone plays in the health status of the afflicted joint.

The invention discloses a femoral head region bone feature segmentation method using SAM-Med3d, and belongs to the field of medical image segmentation optimization. According to a preoperative three-dimensional CT image of an SCFE patient, disease test CT is obtained, data labeling of thighbone, femoral head epiphyseal edge, femoral head and subchondral bone is carried out on part of disease test CT, a labeling result is obtained, data resampling and data scale adjustment are carried out according to the space proportion of a target area, a resampling image of each labeling label is obtained, and the resampling image of each labeling label is obtained. The method comprises the following steps of: putting the four tags and a resampling image of an original image corresponding to the four tags into SAM-Med3d for training to obtain segmentation results of the four tags, performing resampling to restore to an original scale, and combining the four tags together to obtain a final bone feature segmentation result. According to the method, the femur, the femoral head epiphyseal edge, the femoral head and the subchondral bone area in the preoperative three-dimensional CT data of the SCFE patient can be automatically segmented, and compared with original SAM-Med3d, the segmentation accuracy can be improved, and the method has important significance for subsequent operation scheme design.

The invention provides an animal osteochondral injury impactor. The animal osteochondral injury impactor comprises a main body module, an operation module, an impact module and an adjusting module. An impact hole and an installation hole are formed in the two opposite ends of the main body module and communicate with each other through an installation cavity in the main body module. The side wall of the main body module is provided with a sliding groove and an operation hole which are both communicated with the installation cavity. The sliding grooves and the operation holes are formed in the outer wall of the main body module at intervals. The operation module comprises a trigger, a first pin and a reset piece. The trigger is rotationally connected to the hole wall of the operation hole through a first pin, and the two opposite ends of the trigger are provided with a clamping hook and an operation arm correspondingly. By means of force storage and force adjustment, more real impact injury can be generated on the cartilage surface of an animal bone, a clinical real injury environment is simulated, impact energy is transmitted to a subchondral bone from the cartilage after being generated on the cartilage surface, and then injury to the cartilage and the subchondral bone can be generated. The accuracy and the high efficiency of osteochondral injury research are favorably improved.

The invention discloses a double-layer bionic composite scaffold for osteochondral defect integrated repair and a preparation method thereof.The scaffold comprises an integrated scaffold upper layer and an integrated scaffold lower layer, the integrated scaffold upper layer is a hydrogel layer for repairing a cartilage layer, HAMA and ChsMA are introduced into the cartilage repairing layer of the scaffold, and TGF-beta3 is compounded to form composite hydrogel, so that the composite scaffold is obtained. The cartilage layer has biological activity and degradation performance; the lower layer of the integrated scaffold is a Col I scaffold layer for repairing a subchondral bone layer, nHA and Col I are mixed in the Col I scaffold layer, BMP-2 is added, and the scaffold with high mechanical strength and good osteoconductivity is prepared in a freeze-drying mode. An integrated structure is formed between the cartilage repair layer and the bone repair layer of the composite scaffold, and the concentration and molecular weight of the hydrogel and the collagen scaffold are regulated and controlled, so that the degradation rate of the repair layer and the slow release control of bioactive factors can be realized. The composite scaffold not only promotes the regeneration of cartilage and subchondral bone, but also can improve the mechanical properties and functions of new tissues.

The invention discloses a three-layer repair scaffold and a preparation method and application thereof, and the three-layer repair scaffold comprises a first repair layer containing a first matrix and a first active component dispersed in the first matrix, a second repair layer containing a second matrix and a second active component dispersed in the second matrix, and a middle connecting layer, the first repairing layer is located between the first repairing layer and the second repairing layer and connected with the first matrix and the second matrix through intermolecular force; the first active component and the second active component are coated in the same or different sustained-release materials. The three-layer repair scaffold provided by the invention can better promote mesenchymal stem cells to differentiate towards cartilage and osteogenesis in different repair layers, so that the injury of cartilage and subchondral bone can be more effectively repaired.

The invention relates to the field of tissue engineering biological materials, in particular to a preparation method and application of keratin-based composite osteochondral, and according to the preparation method, a cartilage layer 1, a calcified cartilage layer 2 and a subchondral bone layer 3 are combined into the composite osteochondral. The cartilage layer 1 is keratin-based nanofiber aerogel prepared through an electrostatic spinning method and a foaming technology, the calcified cartilage layer 2 is a keratin nanofiber membrane which is prepared through the electrostatic spinning method and is rich in calcium and phosphorus ions, and the subchondral bone layer 3 is a modified keratin-based hydrogel material prepared through a 3D printing technology. The three layers of structures are combined in sequence to form the keratin-based composite osteochondral bone. The prepared keratin-based scaffold has good biocompatibility, is beneficial to adhesion and proliferation of bone cells, and has the function of promoting repair and regeneration of bone cartilage.

The present invention relates generally to minimally invasive, cost-effective, adaptable methods, systems, and devices used to repair anatomical joint conditions. The repair may be necessitated by trauma, disease or other conditions. The anatomical joint may specifically include mammalian joints such as the knee, shoulder, elbow, wrist, finger, hip, spine, toe and ankle, for example. The methods, systems, and devices disclosed herein include leveraging the significant (and often unappreciated) role the subchondral bone plays in the health status of the afflicted anatomical joint.

A glenoid implant including a base plate and an articular component. The base plate can include a body and a support structure extending from a distal surface of the body. The body can include a plurality of openings. The articular component can be configured to removably couple to the base plate. The articular component can include a recessed portion configured to at least partially receive the body of the base plate. At least one engagement structure can protrude from a distal facing surface of the recessed portion. Each engagement structure can correspond to one of the plurality of openings in the body. A distal face of the articular component surrounding the recessed portion can be configured to abut the subchondral bone.

The invention belongs to the technical field of bone tissue engineering and regenerative medicine, and particularly discloses a piezoelectric-photo-thermal dual-mode composite stent for articular cartilage repair and a preparation method of the piezoelectric-photo-thermal dual-mode composite stent. The preparation method comprises the three steps of MXene preparation, electrostatic spinning and paper folding structure construction, poly-L-lactic acid and MXene nanosheets are compounded to prepare a fiber membrane, and then the fiber membrane is subjected to hot pressing through a corrugated mold to form the three-dimensional stent with the periodic creased structure. The scaffold has piezoelectric property and photo-thermal property at the same time, promotes cartilage differentiation through the piezoelectric effect, inhibits subchondral bone angiogenesis through the photo-thermal effect, maintains a low-oxygen microenvironment, realizes integral repair of cartilage-subchondral bone functional units, can be used for treatment of cartilage defects, osteochondral defects and osteoarthritis, and has a wide application prospect. The problem that the cartilage steady state is damaged due to the fact that a traditional piezoelectric material promotes bone vascularization is solved.

The application provides a kind of osteochondral tissue repair scaffold and its preparation method.The osteochondral tissue repair scaffold is made by self-made photo-curing 3D printing equipment integration, including bionic double-layer structure: cartilage layer and subchondral bone layer, cartilage layer has "lotus root-like" and "radiation-like" hole, subchondral bone layer has "lotus root-like" hole, and longitudinal interstices between cartilage layer and subchondral bone layer are mutually through, aperture is accurately controlled, and also can add calcium phosphate nanocluster material with 1 nanometer particle size in subchondral bone layer.The osteochondral tissue repair scaffold prepared by the application aims at the different tissue characteristics and regeneration requirements of cartilage and subchondral bone, designs double-layer scaffold with different physical and biological characteristics, simulates the anisotropy of natural osteochondral structure, promotes cell migration and tissue growth, and achieves the effect of promoting synchronous regeneration of cartilage and subchondral bone, so it has great application potential.

The application relates to a cartilage repair bracket, which comprises subchondral bone and cartilage attached to the subchondral bone, the attachment mode comprising the use of sutures, biocompatible adhesives, porous absorbable polymer layers and / or absorbable fasteners, the subchondral bone comprises a multi-stage porous metal material, and the subchondral bone comprises a first surface and a second surface. The cartilage repair bracket has the advantages of good fusion, stable structure and the like.

The application relates to the technical field of biological medicine, and discloses application of wormwood exosome in prevention and treatment of joint diseases. The wormwood exosome has the advantages of wide source, low extraction cost, high safety, good biocompatibility and definite biological effect, can realize targeted treatment of arthritis, effectively delays joint cartilage degeneration, reduces the pain reaction, and improves pathological remodeling of the subchondral bone, provides a new technical path and treatment strategy for intervention of arthritis, and has wide application prospects.

This invention relates to the field of biomedical technology, specifically providing a product for the treatment and diagnosis of osteoarthritis (OA) targeting Y-box-binding protein 1 (YBX1) and its applications. The method utilizes a recombinant adeno-associated virus (AAV) vector to deliver a YBX1 interference sequence, which is then injected locally into the knee joint to specifically target subchondral bone marrow macrophages (BMMs). Experiments have demonstrated that this method effectively inhibits osteoclast differentiation of BMMs, alleviates pain in OA model mice, improves abnormal bone remodeling and bone loss in the subchondral bone, and delays degeneration of the overlying cartilage, while exhibiting good biocompatibility. This invention provides a novel strategy and drug candidate for the etiological treatment of OA.

A photocurable 3D-printed bioceramic osteochondral scaffold, comprising a cartilage repair layer and a subchondral bone repair layer that are formed of the same material, wherein the cartilage repair layer has a porous structure having a first pore size, and the first pore size is 100-1000 µm; the subchondral bone repair layer has a porous structure having a second pore size, and the second pore size is 300-1000 µm; the cartilage repair layer and the subchondral bone repair layer interdigitate at the interface to form a porous structure having a third pore size, and the third pore size is 50-300 µm; the third pore size is less than the first pore size and the second pore size. The osteochondral scaffold can not only ensure the infiltration of bone marrow blood carrying bioactive substances such as bone marrow mesenchymal stem cells and growth factors into cartilage defect sites, but also prevent vascular invasion from the subchondral bone repair layer, thereby inhibiting calcification of the cartilage layer; and the subchondral bone repair layer provides sufficient support for the repair of the cartilage layer during the repair process.

The invention relates to the technical field of biological medicines, and particularly provides a Y-frame binding protein 1 (YBX1)-targeted osteoarthritis treatment and diagnosis product and application thereof. According to the method, a YBX1 interference sequence is delivered by using a recombinant adeno-associated virus (AAV) vector, and subchondral bone marrow macrophages (BMMs) are specifically targeted through knee joint local injection. Experiments prove that the method can effectively inhibit osteoclast differentiation of BMMs, relieve pain of OA model mice, improve abnormal bone remodeling and bone loss of subchondral bones and delay degeneration of overlying cartilage, and shows good biological safety at the same time. The invention provides a new strategy and drug candidate for etiological treatment of OA.