82 results about "Basic fibroblast growth factor" patented technology

The present invention relates to the field of stem cell technology. The present invention discloses a method for inducing stem cells to secrete specific factors, which includes stimulating stem cells with high-frequency waves, emitting sound waves of a specific frequency to stem cells, regulating stem cells in a hypoxic environment, culturing stem cells in a hypoxic environment, stimulating stem cells with oxidative stress, and subjecting stem cells to oxidative stimulation treatment. The present invention can significantly enhance the proliferation ability of stem cells, promote the secretion of multiple active factors, and improve cell function and adaptability. As a non-invasive physical method, high-frequency wave stimulation can activate internal cell signal pathways without introducing exogenous substances, significantly improving the proliferation ability and tissue repair potential of stem cells. Hypoxic environment regulation simulates the naturally existing microenvironmental conditions in the body, greatly promoting the secretion of active factors such as vascular endothelial growth factor, basic fibroblast growth factor, and brain-derived neurotrophic factor for tissue repair and immune regulation.

The invention relates to morin nanoparticles, pH responsive hydrogel and application of the morin nanoparticles and the pH responsive hydrogel. The morin-loaded nanoparticles provided by the invention can be rapidly degraded in an alkaline environment, have certain stability in an acidic environment, have the capability of releasing morin in response to pH, and can realize accurate regulation and control of morin release according to the change of environmental pH. According to the invention, hyaluronic acid is further subjected to methacrylation treatment, such that MeHA is obtained. The characteristic that MeHA is easier to degrade in an acid environment is utilized, and MeHA is used as hydrogel to load CFMNPs and can be effectively used for treating wounds caused by burns and frostbite. Furthermore, the pH responsive double-layer hydrogel based on pigment nanoparticles-basic fibroblast growth factors can realize sequential release of the effective components according to the change of the pH value of the burn or frostbite wound. The composition can effectively inhibit inflammation, resist oxidative stress, promote proliferation and migration of endothelial cells, promote neovascularization and finally accelerate wound healing.

The present invention discloses a low - serum medium and its application in the preparation of cultured meat from Larimichthys crocea cells, which is also applicable to other marine or freshwater fish, belonging to the field of bioengineering technology. The low - serum medium comprises a basal medium, fetal bovine serum accounting for 30 - 80 μL / mL, and exogenous additives. The exogenous additives include human serum albumin, ascorbic acid, basic fibroblast growth factor polypeptide, human holo - transferrin, lysophosphatidic acid, and human recombinant IGF - 1. Based on the DMEM / F12 medium, the low - serum medium provided by the present invention adds six substances that promote cell growth, reduces the dependence of satellite cells on fetal bovine serum, effectively reduces the usage amount of fetal bovine serum, and saves the culture cost. Compared with the general - type medium (DMEM high - glucose, adding 10% FBS), the low - serum medium provided by the present invention has an equivalent effect on the maintenance and proliferation ability of cell stem cells.

The invention relates to the technical field of culture media, in particular to a serum-free complete culture medium for human umbilical cord mesenchymal stem cells and a preparation method thereof.The serum-free complete culture medium takes DMEM / F12 as a basic culture medium and is matched with UltraGRO-Advanced, a penicillin-streptomycin double-antibody solution, a recombinant human epidermal growth factor and a basic fibroblast growth factor, and the serum-free complete culture medium for the human umbilical cord mesenchymal stem cells is obtained. The serum-free complete culture medium can replace a serum culture medium in a conventional culture system of mesenchymal stem cells, the characteristics of normal adherence, good growth state and stem cells of the cells are kept, the serum-free complete culture medium reduces the risks of heterologous contamination and immunological rejection to the maximum extent, the added components capable of promoting cell growth and the content can be determined and controlled, and the culture medium is suitable for large-scale popularization and application. The use is safer.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention relates to an external degradable microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: S1, preparing powdery CS-TA; s2, preparing a powdery Cu-TA nano enzyme; s3, adding the Cu-TA nano-enzyme and glucose oxidase GOD into water, stirring at 1-4 DEG C in an inert gas atmosphere, centrifuging, and re-suspending in water to obtain a GOD-coated Cu-TA compound enzyme solution; s4, adding CS-TA, hyaluronic acid, polyvinylpyrrolidone, L-arginine and a basic fibroblast growth factor into the GOD (at) Cu-TA compound enzyme solution, and preparing a microneedle mother solution; s5, preparing an aqueous solution containing CS-TA, hyaluronic acid and polyvinylpyrrolidone to obtain a substrate mother solution; and S6, filling a microneedle mold cavity of a mold with the microneedle mother solution to form enzyme series microneedles arranged in an array, then pouring a substrate mother solution to form a substrate, drying, and demolding to obtain the external degradable microneedle patch.

The present application relates to the technical field of biology, and particularly relates to a mesenchymal stem cell culture medium and a preparation method and application thereof.The mesenchymal stem cell culture medium comprises a basic culture medium, fetal bovine serum, basic fibroblast growth factor, polymyxin B and deionized water.The present application can simultaneously improve the proliferation activity of mesenchymal stem cells and the secretion performance of HGF by simultaneously adding the basic fibroblast growth factor and the polymyxin B in the basic culture medium; more stem cells can be in the active proliferation and division process by limiting the content of the basic fibroblast growth factor in the mesenchymal stem cell culture medium to 5-50 ng / mL; and the secretion performance of HGF can be improved while the cell proliferation activity is maintained by limiting the content of the polymyxin B in the mesenchymal stem cell culture medium to 50-100 mu g / mL.

The invention discloses a serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and application thereof. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition is prepared from the following components in parts by mass: 15 to 25 parts of platelet-derived growth factor-BB; 15 to 25 parts of basic fibroblast growth factors; 8 to 12 parts of transforming growth factor-beta; and 8-12 parts of recombinant human insulin. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and the application thereof disclosed by the invention have the following beneficial effects: (1) the proliferation efficiency is remarkably improved; (2) the cell morphology and dryness can be well maintained; and (3) the compliance and the compatibility are good.

The invention provides a special culture medium for prostate cancer organoid and a culture method thereof, and belongs to the technical field of biological medicines. The special culture medium for the prostatic cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium does not contain exogenously added Noggin recombinant protein and R-spondin recombinant protein. The culture additive is prepared from a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, a p38MAPK signal channel inhibitor, an insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, a ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, a eriocin and androgen. According to the special culture medium for the prostatic cancer organoid, the culture success rate is increased while the cost is reduced.

The present invention provides a special culture medium for breast cancer organoids and a culture method thereof, belonging to the technical field of primary cell culture. A special culture medium for breast cancer organoids is composed of a basal medium and culture additives. The basal medium is DMEM / F12 medium, and the culture additives are composed of epidermal growth factor, basic fibroblast growth factor, keratinocyte growth factor, fibroblast growth factor 10, insulin, transferrin, sodium selenite, A83-01, SB202190, insulin-like growth factor 1, B27, nicotinamide and N-acetylcysteine. The culture medium of the present invention improves the stability of the tumor cell genome during the culture while reducing the culture cost.

The invention relates to the field of medical beauty industry, and particularly discloses intelligent response type hydroxyapatite medical beauty gel as well as a preparation method and application thereof. The intelligent response type hydroxyapatite medical beauty gel is prepared from the following raw material components: 90 to 95 parts of hydroxyapatite, 35 to 40 parts of regenerated silk fibroin composite magnetic microspheres, 50 to 55 parts of hyaluronic acid, 20 to 25 parts of recombinant III type humanized collagen and 15 to 20 parts of basic fibroblast growth factor sustained release microspheres. The preparation method comprises the following steps: uniformly dispersing all the components in hyaluronic acid, and stirring to obtain the medical beauty gel. The intelligent response type hydroxyapatite medical beauty gel belongs to an important gel material in orthopedic repair in the medical beauty industry, can well repair a bone defect part, and has excellent osteogenesis performance, biocompatibility and biological safety in vivo; and the product has the great advantages of high safety and good beautifying effect in medical beautifying bone shaping and bone repair.

The application discloses a temperature-sensitive hydrogel with a diabetes treatment effect, a kit, a use method and application thereof, and belongs to the technical field of diabetes treatment drugs. The temperature-sensitive hydrogel is formed by mixing a human umbilical vein endothelial cell suspension, a mixed solution with a final concentration of 15-20% of P407 and a final concentration of 0.6-0.8% of sodium hyaluronate, a final concentration of 50 ng / mL of vascular endothelial growth factor, a final concentration of 10 ng / mL of basic fibroblast growth factor, a final concentration of 0.5-2% of lidocaine hydrochloride, a final concentration of 100-200 mg / mL of tumor necrosis factor alpha monoclonal antibody and a final concentration of 2-5% of islet cell-alginic acid gel fiber segments with a length of less than 1 mm. The temperature-sensitive hydrogel is safe, non-toxic, low in immunogenicity, low in cost and good in treatment effect. The reagent is combined with sterile consumables to form a kit, and the kit is convenient to use and can be better applied to the treatment of diabetes.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

The present invention relates to a method for producing neuronal cells from dedifferentiated adipocytes derived from a mammal, the method comprising a step for culturing dedifferentiated adipocytes derived from a mammal using a medium that contains a basic fibroblast growth factor (bFGF), retinoic acid, and a neuronal differentiation inducer, but does not substantially contain an epigenetic inhibitor.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The invention discloses a culture medium for in-vitro adherent culture of primary tumor cells and application, and relates to the technical field of tumor cell culture. Comprising a basic culture medium, fetal calf serum FBS with the concentration of 5 ng / mL-15 ng / mL, an epidermal growth factor EGF with the concentration of 1 ng / mL-10 ng / mL, a basic fibroblast growth factor bFGF with the concentration of 0.5 ng / mL-5 ng / mL, an insulin-transferrin-selenium ITS supplement with the final volume concentration of 0.1%-1%, L-glutamine with the final volume concentration of 0.5 mM-2 mM, ascorbic acid with the final volume concentration of 0.1%-0.5% and non-essential amino acid with the final volume concentration of 1%-5%. The kit comprises 10 [mu] g / mL-50 [mu] g / mL of heparin and 1 [mu] g / mL-5 [mu] g / mL of an Rho kinase inhibitor. The culture medium overcomes the defects that in the prior art, when primary tumor cells are subjected to adherent culture, apoptosis or dedifferentiation is prone to occurring, in-vivo tumor characteristics are difficult to simulate, the tumor growth speed is low, the proliferation effect is poor, and the adherent rate is low.

The present invention provides a culture medium for culturing primary gastric cancer cells, comprising an MST1 / 2 kinase inhibitor, a ROCK kinase inhibitor, at least one of a B27 additive and an N2 additive, basic fibroblast growth factor, CHIR99021, epidermal growth factor, an ITS cell culture additive, SB202190, dexamethasone, fibroblast growth factor 10, N-acetyl-L-cysteine, and gastrin. The present invention also relates to a culture method using the primary cell culture medium, wherein the culture medium is used to culture the primary cells on a culture vessel coated with extracellular matrix glue, thereby rapidly proliferating the primary cells.

The present invention provides an improved method for culturing mesenchymal stem cells and a culture medium used therefor, belonging to the technical field of cell culture. The improved mesenchymal stem cell culture medium provided by the present invention comprises the following exogenous additive components: recombinant human serum albumin, recombinant transferrin, recombinant human epidermal growth factor, basic fibroblast growth factor, platelet-derived growth factor, insulin, L-glutamine, reduced glutathione, β-mercaptoethanol, sodium selenite, penicillin-streptomycin, allicin, heparin and a proliferation promoter; the proliferation promoter comprises paeoniflorin, total saponins of Panax notoginseng, wolfberry polysaccharide and alcohol extract of Saussurea involucrata. By using the above culture medium for culturing mesenchymal stem cells, the cell activity and proliferation ability of mesenchymal stem cells can be significantly increased, the cell viability is increased by more than 50%, and the cell proliferation ability is increased by more than 40%, having great application potential in the culture of mesenchymal stem cells.

The invention relates to the technical field of regenerative medicine application, in particular to a serum-free induction culture medium with definite components for promoting adipose tissue-derived cells to be differentiated into dermal papilla cells, an induction method and application. The invention relates to a method for promoting adipose tissue-derived cells to differentiate into dermal papilla cells, which is characterized in that the adipose tissue-derived cells are induced and cultured by using a serum-free induction culture medium, so that the adipose tissue-derived cells are differentiated into the dermal papilla cells. The formula of the serum-free induction culture medium comprises hepatocyte growth factors, hydrocortisone, bone morphogenetic protein 2, BIO and basic fibroblast growth factors. Based on the differentiation potential of adipose tissue-derived mesenchymal cells and the function maintenance mechanism of dermal papilla cells, a serum-free induction system with definite components is constructed, and directional differentiation is realized. According to the technical scheme, the technical problem that a method for inducing the adipose-derived mesenchymal cells to be directionally differentiated into the dermal papilla cells with the effective hair follicle regulation function is lacked in the prior art can be solved, and the method has ideal application and popularization potential.

The invention discloses hydrogel capable of releasing growth factors after heat absorption and contraction and a preparation method of the hydrogel, and belongs to the field of biomedical materials. The hydrogel is of a three-layer composite structure, wherein the first layer is a sodium alginate-chitosan polyelectrolyte semi-interpenetrating network layer and serves as a compact protective layer; the second layer is a bacterial cellulose-sodium alginate high-toughness layer and is used as a bearing layer and a shrinkage driving layer; and the third layer is a basic fibroblast growth factor (bFGF)-loaded poly (butylene adipate / terephthalate) (PBAT) electrostatic spinning film layer and is used as a medicine storage cavern. According to the invention, the characteristic that the bacterial cellulose / sodium alginate layer can generate reversible heat absorption volume shrinkage at a specific temperature is used as a physical driving force to extrude and promote the bFGF in the third layer of PBAT fiber membrane to directionally release outwards.

The invention relates to the technical field of protein purification, and provides a method for eukaryotic purification of alpha-syn A53T protein through HEK293 cells. The method comprises the following steps: constructing a recombinant expression plasmid containing an SNCA-A53T mutation target fragment, inserting an affinity tag, transferring into escherichia coli, and performing amplification expression to obtain an endotoxin-free superhelix plasmid for transfection; inoculating HEK293 cells, and adding L-glutamine into the culture medium; the preparation method comprises the following steps: adding endotoxin-free superhelix plasmids and polyethyleneimine into HEK293 cells in sequence, uniformly mixing, standing, adding into the HEK293 cells, and supplementing feed Feed-1, L-glutamine, glucose, epidermal growth factors (EGF) and basic fibroblast growth factors (bFGF); agarose magnetic beads are specifically combined with an affinity tag, and then the magnetic beads are washed and eluted to obtain the purified alpha-syn A53T protein. A supplementary material Feed-1, an epidermal growth factor (EGF) and a basic fibroblast growth factor (bFGF) are introduced in an induced expression stage, so that the expression quantity and the purity of the alpha-syn A53T protein are effectively improved, and the structural stability and the functional integrity of the alpha-syn A53T protein are enhanced.

The invention relates to the technical field of biological pharmacy, and discloses a mesenchymal stem cell exosome enhancer and a preparation method thereof, the enhancer is composed of purified mesenchymal stem cell exosome, a functional lipid complex, cytokine sustained release microspheres, a targeting modifier, a stabilizer and a penetration enhancer according to specific parts by weight. The preparation method comprises the following steps: obtaining a high-activity exosome by combining ultracentrifugation with size exclusion chromatography purification; preparing a functional lipid complex composed of cholesterol, sphingomyelin and phosphatidylserine to promote membrane fusion; preparing a sodium alginate-chitosan sustained release microsphere loaded with a basic fibroblast growth factor and a transforming growth factor-beta1; synthesizing an RGD peptide modified hyaluronic acid targeting modifier; and finally, mixing the components, filtering and sub-packaging. The quality fluctuation risk of subsequent preparation preparation is reduced, and it is guaranteed that the final reinforcing agent product has a highly uniform structure.

The present invention relates to a multifunctional bionic hydrogel and its preparation method and application, belonging to the technical field of hydrogel preparation. The present invention discloses a multifunctional bionic hydrogel, which is composed of fibrin (Fn), HA-SH-DA (a product obtained by the amide reaction of thiol, dopamine (DA) and hyaluronic acid (HA)), Ag<supgt;+< / supgt; and thrombin (containing basic fibroblast growth factor and alanyl glutamine). In the multifunctional bionic hydrogel of the present invention, Ag<supgt;+< / supgt>, HA-SH-DA, fibrinogen and thrombin interact with each other to form a hydrogel with a double-network interweaving structure; the multifunctional bionic hydrogel provided by the present invention gradually degrades in the acidic intestinal environment under ulcerative colitis (UC) conditions and continuously releases therapeutic contents, making it have good application prospects in the preparation of drugs for treating ulcerative colitis.

The invention relates to the technical field of biological medicines, and provides a high VEGF protein / miR-210 extracellular vesicle induced by a serum-free combined culture medium and application of the high VEGF protein / miR-210 extracellular vesicle. According to the invention, high VEGF protein / miR-210 extracellular vesicles are obtained based on induction of a serum-free culture medium; the serum-free culture medium comprises a basic culture medium component combination: sugar-free RPMI1640 and MCDB131 in a volume ratio of 4: 1; the metabolic regulation additive is prepared by synergistically adding sodium pyruvate, cysteine and a proline hydroxylase inhibitor and taking alanine glutamine as an energy substitute; the growth factor is a combination of polyvinyl alcohol, 4-hydroxyethylpiperazine ethanesulfonic acid, ITS-X, epidermal growth factors and basic fibroblast growth factors, wherein the ITS-X, the epidermal growth factors and the basic fibroblast growth factors are added before use. CD63 and CD81 are specifically expressed on the surface of the prepared high VEGF protein / miR-210 extracellular vesicle, the high VEGF protein / miR-210 extracellular vesicle is rich in VEGF protein and miR-210, and the high VEGF protein / miR-210 extracellular vesicle is applied to targeted therapy of allergic rhinitis by inhibiting Th2 type immunoreaction.

Provided are novel serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3 and ascorbic acid at a concentration of at least about 50 microgram / ml; ascorbic acid at a concentration range of about 400-600 microgram / ml, bFGF at a concentration range of about 50-200 ng / ml, xeno-free serum replacement and a lipid mixture; the IL6RIL6 chimera at a concentration range of about 50-200 picogram per milliliter (pg / ml); or leukemia inhibitory factor (LIF) at a concentration of at least 2000 units / ml; cell cultures comprising same with pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems; and methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation.

The invention discloses an umbilical cord mesenchymal stem cell culture medium and application thereof, and particularly relates to the technical field of cell culture. The umbilical cord mesenchymal stem cell culture medium is prepared from the following components: 10 to 40 ng / mL of human basic fibroblast growth factors, 55 to 95 ng / mL of ambrotin, 22 to 53 ng / mL of porphyrin sodium, 2 to 5 [mu] g / mL of transferrin, 6 to 11 mM of L-glutamine, 45 to 90 [mu] M of beta-mercaptoethanol, 8 to 12 [mu] g / mL of insulin, 7 to 13 [mu] g / mL of L-glutathione and a DMEM / F12 culture medium. In addition, the culture medium can effectively improve the proliferation activity and osteogenic differentiation potential of the umbilical cord mesenchymal stem cells, and is expected to make an important contribution to the development of the fields of regenerative medicine and tissue engineering.

The invention relates to the technical field of stem cell culture. The invention provides a stem cell three-dimensional culture medium and application thereof. The culture medium comprises a basic culture medium and an additive, the additives comprise collagen, Pluronic F-68, hyaluronic acid, folic acid, basic fibroblast growth factors, linoleic acid, arachidonic acid, platelet-derived growth factors, epidermal growth factors, insulin-like growth factor-1, SCF stem cell factors, thrombopoietin, vitamin C, vitamin E, vitamin B1, vitamin B2 and vitamin B6. The culture medium provided by the invention does not need a three-dimensional carrier, and stem cells can be directly cultured in a suspension culture mode. Meanwhile, cell adherence can be effectively prevented, collagenase is not needed in subsequent treatment, the in-vitro culture amplification speed and the cell survival rate are further increased, and the method is suitable for large-scale production and preparation of stem cells.

The invention discloses an efficient amplification process and anti-aging application of human-derived amniotic mesenchymal stem cells, and belongs to the technical field of stem cell culture. Aiming at the problems of slow cell proliferation, easy aging and pluripotency loss caused by traditional two-dimensional culture, efficient amplification is realized by constructing a modified polylactic acid-glycolic acid-chondroitin sulfate gel three-dimensional scaffold and combining with an optimized culture medium. The scaffold is formed by cross-linking a norbornene modified polylactic acid-glycolic acid copolymer and tetrazine functionalized chondroitin sulfate through an anti-Diels-Alder reaction, a natural extracellular matrix structure is simulated, the cell attachment rate is remarkably increased, and the doubling time is shortened. The culture medium contains methacrylated hyaluronic acid, growth factors and a serum substitute to synergistically support cell activity. The three-dimensional scaffold has a pH-responsive degradation characteristic, can realize biphasic slow release of basic fibroblast growth factors and platelet-derived growth factors, and maintains the dryness and differentiation potential of stem cells.

The invention discloses a skin lesion repairing ointment and a preparation method thereof, and belongs to the technical field of medicines, and the skin lesion repairing ointment is prepared from the following raw materials: periplaneta americana, purslane, golden cypress, scutellaria baicalensis and radix sophorae flavescentis. Tests show that the skin lesion repairing ointment has the same curative effect as the compound dexamethasone acetate cream and the recombinant bovine basic fibroblast growth factor gel on skin lesion treatment, and the curative effect is more lasting. However, the specific action mechanism is still unclear, and further research is needed. In the aspect of safety, the skin lesion repairing ointment group and the compound dexamethasone acetate cream and recombinant bovine basic fibroblast growth factor gel group do not have adverse reaction, so that the external ointment and the compound dexamethasone acetate cream and recombinant bovine basic fibroblast growth factor gel group are relatively safe.