29 results about "Basic fibroblast growth factor" patented technology

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The present application relates to the technical field of biology, and particularly relates to a mesenchymal stem cell culture medium and a preparation method and application thereof.The mesenchymal stem cell culture medium comprises a basic culture medium, fetal bovine serum, basic fibroblast growth factor, polymyxin B and deionized water.The present application can simultaneously improve the proliferation activity of mesenchymal stem cells and the secretion performance of HGF by simultaneously adding the basic fibroblast growth factor and the polymyxin B in the basic culture medium; more stem cells can be in the active proliferation and division process by limiting the content of the basic fibroblast growth factor in the mesenchymal stem cell culture medium to 5-50 ng / mL; and the secretion performance of HGF can be improved while the cell proliferation activity is maintained by limiting the content of the polymyxin B in the mesenchymal stem cell culture medium to 50-100 mu g / mL.

The invention discloses a serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and application thereof. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition is prepared from the following components in parts by mass: 15 to 25 parts of platelet-derived growth factor-BB; 15 to 25 parts of basic fibroblast growth factors; 8 to 12 parts of transforming growth factor-beta; and 8-12 parts of recombinant human insulin. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and the application thereof disclosed by the invention have the following beneficial effects: (1) the proliferation efficiency is remarkably improved; (2) the cell morphology and dryness can be well maintained; and (3) the compliance and the compatibility are good.

The invention relates to the field of medical beauty industry, and particularly discloses intelligent response type hydroxyapatite medical beauty gel as well as a preparation method and application thereof. The intelligent response type hydroxyapatite medical beauty gel is prepared from the following raw material components: 90 to 95 parts of hydroxyapatite, 35 to 40 parts of regenerated silk fibroin composite magnetic microspheres, 50 to 55 parts of hyaluronic acid, 20 to 25 parts of recombinant III type humanized collagen and 15 to 20 parts of basic fibroblast growth factor sustained release microspheres. The preparation method comprises the following steps: uniformly dispersing all the components in hyaluronic acid, and stirring to obtain the medical beauty gel. The intelligent response type hydroxyapatite medical beauty gel belongs to an important gel material in orthopedic repair in the medical beauty industry, can well repair a bone defect part, and has excellent osteogenesis performance, biocompatibility and biological safety in vivo; and the product has the great advantages of high safety and good beautifying effect in medical beautifying bone shaping and bone repair.

The application discloses a temperature-sensitive hydrogel with a diabetes treatment effect, a kit, a use method and application thereof, and belongs to the technical field of diabetes treatment drugs. The temperature-sensitive hydrogel is formed by mixing a human umbilical vein endothelial cell suspension, a mixed solution with a final concentration of 15-20% of P407 and a final concentration of 0.6-0.8% of sodium hyaluronate, a final concentration of 50 ng / mL of vascular endothelial growth factor, a final concentration of 10 ng / mL of basic fibroblast growth factor, a final concentration of 0.5-2% of lidocaine hydrochloride, a final concentration of 100-200 mg / mL of tumor necrosis factor alpha monoclonal antibody and a final concentration of 2-5% of islet cell-alginic acid gel fiber segments with a length of less than 1 mm. The temperature-sensitive hydrogel is safe, non-toxic, low in immunogenicity, low in cost and good in treatment effect. The reagent is combined with sterile consumables to form a kit, and the kit is convenient to use and can be better applied to the treatment of diabetes.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The present invention relates to a method for producing neuronal cells from dedifferentiated adipocytes derived from a mammal, the method comprising a step for culturing dedifferentiated adipocytes derived from a mammal using a medium that contains a basic fibroblast growth factor (bFGF), retinoic acid, and a neuronal differentiation inducer, but does not substantially contain an epigenetic inhibitor.

The invention discloses hydrogel capable of releasing growth factors after heat absorption and contraction and a preparation method of the hydrogel, and belongs to the field of biomedical materials. The hydrogel is of a three-layer composite structure, wherein the first layer is a sodium alginate-chitosan polyelectrolyte semi-interpenetrating network layer and serves as a compact protective layer; the second layer is a bacterial cellulose-sodium alginate high-toughness layer and is used as a bearing layer and a shrinkage driving layer; and the third layer is a basic fibroblast growth factor (bFGF)-loaded poly (butylene adipate / terephthalate) (PBAT) electrostatic spinning film layer and is used as a medicine storage cavern. According to the invention, the characteristic that the bacterial cellulose / sodium alginate layer can generate reversible heat absorption volume shrinkage at a specific temperature is used as a physical driving force to extrude and promote the bFGF in the third layer of PBAT fiber membrane to directionally release outwards.

The invention relates to the technical field of protein purification, and provides a method for eukaryotic purification of alpha-syn A53T protein through HEK293 cells. The method comprises the following steps: constructing a recombinant expression plasmid containing an SNCA-A53T mutation target fragment, inserting an affinity tag, transferring into escherichia coli, and performing amplification expression to obtain an endotoxin-free superhelix plasmid for transfection; inoculating HEK293 cells, and adding L-glutamine into the culture medium; the preparation method comprises the following steps: adding endotoxin-free superhelix plasmids and polyethyleneimine into HEK293 cells in sequence, uniformly mixing, standing, adding into the HEK293 cells, and supplementing feed Feed-1, L-glutamine, glucose, epidermal growth factors (EGF) and basic fibroblast growth factors (bFGF); agarose magnetic beads are specifically combined with an affinity tag, and then the magnetic beads are washed and eluted to obtain the purified alpha-syn A53T protein. A supplementary material Feed-1, an epidermal growth factor (EGF) and a basic fibroblast growth factor (bFGF) are introduced in an induced expression stage, so that the expression quantity and the purity of the alpha-syn A53T protein are effectively improved, and the structural stability and the functional integrity of the alpha-syn A53T protein are enhanced.

The invention relates to the technical field of biological medicines, and provides a high VEGF protein / miR-210 extracellular vesicle induced by a serum-free combined culture medium and application of the high VEGF protein / miR-210 extracellular vesicle. According to the invention, high VEGF protein / miR-210 extracellular vesicles are obtained based on induction of a serum-free culture medium; the serum-free culture medium comprises a basic culture medium component combination: sugar-free RPMI1640 and MCDB131 in a volume ratio of 4: 1; the metabolic regulation additive is prepared by synergistically adding sodium pyruvate, cysteine and a proline hydroxylase inhibitor and taking alanine glutamine as an energy substitute; the growth factor is a combination of polyvinyl alcohol, 4-hydroxyethylpiperazine ethanesulfonic acid, ITS-X, epidermal growth factors and basic fibroblast growth factors, wherein the ITS-X, the epidermal growth factors and the basic fibroblast growth factors are added before use. CD63 and CD81 are specifically expressed on the surface of the prepared high VEGF protein / miR-210 extracellular vesicle, the high VEGF protein / miR-210 extracellular vesicle is rich in VEGF protein and miR-210, and the high VEGF protein / miR-210 extracellular vesicle is applied to targeted therapy of allergic rhinitis by inhibiting Th2 type immunoreaction.

The invention relates to the technical field of organoid culture, and discloses an induction culture medium for culturing a pig lung organoid. Advanced DMEM / F12 is used as a basic culture solution of the culture medium; the invention relates to a basic fibroblast growth factor composition, which is prepared from the following added components with the final concentration: 1500ng / mL of R-Spondinin, 25ng / mL of basic fibroblast growth factor 7 (FGF7), 10100ng / mL of basic fibroblast growth factor, 100ng / mL of Noggin protein, 50ng / mL of epidermal growth factor, 50ng / mL of Wnt3a protein, 500nM of TGF-beta pathway inhibitor, 5mM of ROCK inhibitor, 500nM of p38MAPK inhibitor, 1X of B27 supplement, 1.25 mM of N-acetyl-L-cysteine, 10mM of nicotinamide, 1X of glutamine supplement GlutaMAX1X, 1X of HEPES buffer solution and 1X of broad-spectrum antibiotic (Primocin). According to the method disclosed by the invention, the lung organ with the complex multi-cell lineage is efficiently and stably constructed from the pig lung adult tissue by a one-step method. The obtained organoid is high in biomimetic property and good in stability, provides a tool platform for pig lung development research, disease model construction, drug toxicology testing and regenerative medicine research, and has great scientific research value and wide application prospects.

The present application relates to the technical field of regenerative medicine application, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, and an induction method and application. A method for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, which is to use a serum-free induction medium to induce culture of adipose-derived mesenchymal cells, so that the adipose-derived mesenchymal cells are differentiated into dermal papilla cells. The formula of the serum-free induction medium comprises hepatocyte growth factor, hydrocortisone, bone morphogenetic protein 2, BIO, and basic fibroblast growth factor. The present application is based on the differentiation potential of adipose-derived mesenchymal cells and the mechanism of maintaining the function of dermal papilla cells, and constructs a serum-free induction system with clear components to realize directional differentiation. The technical scheme can solve the technical problem that there is no method for inducing directional differentiation of adipose-derived mesenchymal cells into dermal papilla cells with effective hair follicle regulation function in the prior art, and has ideal application and popularization potential.

The invention discloses a vector, a construction method thereof and application of the vector to FGF (fibroblast growth factor) obtained by expression based on a nicotiana benthamiana chassis, and belongs to the field of basic fibroblast growth factor production. The technical scheme of the invention is as follows: the biomarker comprises a carrier pJL-TRBOGZ082 or a carrier pJL-TRBGZ125; the vector pJL-TRBOGZ082 comprises a 6Xhis-FGF (Fibroblast Growth Factor) sequence as shown in SEQ ID No.1 (Sequence Identifier Number 1); the vector pJL-TRBGZ125 contains a GST (Glutathione S Transferase)-FGF (Fibroblast Growth Factor)-6 Xhis sequence as shown in SEQ ID No. 2. The method is applied to the production of the basic fibroblast growth factor, and solves the technical problems that the related research on the production of the bFGF by utilizing a tobacco transient expression technology at present is not sufficient, and particularly, the key links such as expression vector optimization, permeation infection condition control and protein purification process are still lack of system optimization. The invention provides an efficient and low-cost FGF (Fibroblast Growth Factor) production vector based on a tobacco transient expression system, a construction method of the vector and an application of the vector in obtaining the FGF based on the expression of a nicotiana benthamiana chassis.

The invention belongs to the field of medicine application, and particularly relates to application of polygonum multiflorum and an extract thereof in preparation of a medicine for repairing diabetic wounds. Cell experiments show that the polygonum multiflorum ethanol extract has remarkable in-vitro antioxidant activity and good cell safety, and can effectively enhance proliferation, migration, apoptosis resistance and oxidative stress resistance of human umbilical vein endothelial cells in a high-glucose environment by activating a P62-Keap1-Nrf2 signal channel. Animal experiments further prove that the extracting solution can remarkably accelerate wound healing of diabetic mice, relieve local inflammation infiltration, increase the collagen content, promote transformation of the diabetic mice to mature collagen and reduce the oxidative stress level of tissues at the same time. The treatment effect of the radix polygoni multiflori is equivalent to that of a clinical common drug basic fibroblast growth factor, and the radix polygoni multiflori has a wide application prospect in the aspect of wound repair of diabetes mellitus.

The invention relates to the technical field of information, and particularly provides an automatic sorting method for germline stem cells of plectropomus leopardus, which comprises the following steps: selecting healthy plectropomus leopardus as a raw material, anesthetizing with eugenol, taking gonads, cleaning the gonads with a 1 * PBS buffer solution containing antibiotics and an antifungal agent under an aseptic condition, and drying to obtain germline stem cells of plectropomus leopardus; removing surface blood vessels and coating membranes, and cutting into tissue blocks; the method comprises the following steps: re-suspending gonad cells by using a 1 * PBS buffer solution, adding a predetermined volume of suspension into the 1 * PBS buffer solution mixed with trehalose lipid and glycine betaine in a predetermined ratio, incubating, centrifuging, discarding supernatant, and pre-treating the cells to obtain pre-treated cells; putting the separated germline stem cells into a basal culture medium, adding serum, mercaptoethanol, a GlutaMAX additive, a basic fibroblast growth factor, a leukemia inhibition factor and a glial cell-derived neurotrophic factor, and culturing.

The invention discloses a fusion protein containing a fibroblast growth factor and fibronectin and application of the fusion protein. The fusion protein comprises a fibronectin truncated fragment (such as FNIIIC or FNIIICFC) and a basic fibroblast growth factor (bFGF). The invention also provides a polynucleotide for coding the fusion protein, a recombinant expression vector and a host cell containing the polynucleotide, and a method for purifying the fusion protein through fermentation culture and affinity chromatography. Experiments show that compared with a single component or FN-full, the fusion protein, especially FNIIICFC-b, disclosed by the invention can remarkably promote proliferation and migration of mesenchymal stem cells (MSC) and up-regulate expression of beneficial factors such as HGF (Hepatocyte Growth Factor) and VEGF (Vascular Endothelial Growth Factor). The fusion protein, the pharmaceutical composition containing the fusion protein and the cell culture coating material containing the fusion protein have wide application prospects in the fields of tissue engineering, regenerative medicine and the like.

This invention discloses a recombinant human (or bovine) basic fibroblast growth factor gel with wound-healing and antibacterial functions, and its preparation method. Recombinant human (or bovine) basic fibroblast growth factor gel is a genetically engineered topical drug used clinically to promote healing of burn wounds, chronic wounds, and fresh wounds, with significant efficacy. However, its drawbacks include limited antibacterial properties and inability to be used simultaneously with chemical disinfectants such as iodine and ethanol, which, while promoting wound healing, are not conducive to controlling wound infection. Research has found that protamine sulfate or polylysine, with high safety and good antibacterial properties, can exert good antibacterial and anti-infection functions without negatively affecting the bioactivity and stability of recombinant human (or bovine) basic fibroblast growth factor. This represents an advanced method for preparing recombinant human (or bovine) basic fibroblast growth factor gel with wound-healing and antibacterial functions, enabling this type of drug to better exert its clinical effects and expand its application scope.

The purpose of the present invention is to provide a method whereby hindbrain neural stem cells can be cultured over a long period of time while maintaining multipotency. The present invention relates to a method for manufacturing hindbrain neural stem cells, the method involving: a step for inducing differentiation of hindbrain neural stem cells from pluripotent stem cells; and a step for culturing the hindbrain neural stem cells by using a culture medium that contains a GSK3β inhibitor, a TGFβ inhibitor, and a BMP inhibitor and does not contain a basic fibroblast growth factor (bFGF).

The invention discloses a compound of a basic fibroblast growth factor and a metal organic framework, a pharmaceutical composition and application, and belongs to the technical field of wound healing drugs. The bFGF (at) ZPF-2 compound provided by the invention comprises a metal organic framework material ZPF-2 and bFGF loaded on the ZPF-2, the bFGF (at) MIL-53 (Al)-FA compound provided by the invention comprises a metal organic framework material MIL-53 (Al)-FA and bFGF loaded on the MIL-53 (Al)-FA. By constructing two compounds of bFGF-coated ZPF-2 and bFGF-coated MIL-53 (Al)-FA, efficient loading and activity protection of bFGF are realized, rapid effect taking and continuous release synergy can be realized in response to a wound surface microenvironment, the biocompatibility is good, the wound surface healing effect is remarkably improved, and the storage and transportation cost is reduced.

The invention relates to injectable active bone regeneration hydrogel for delivering bone marrow mesenchymal stem cells as well as a preparation method and application of the injectable active bone regeneration hydrogel, and belongs to the technical field of biological materials. The hydrogel comprises cross-linked methacryloyl chondroitin sulfate (ChSMA) and bacterial cellulose (BC), and basic fibroblast growth factors (bFGF) and bone marrow mesenchymal stem cells (BMSCs) are loaded in the hydrogel. The composite hydrogel formed by crosslinking has balanced and excellent mechanical properties, and has a porous structure beneficial to cell migration and nutrition transportation, so that the basic fibroblast growth factor bFGF loaded in the composite hydrogel can be released more slowly and lastingly; the problems of short half-life period and high-dose inhibition effect of the basic fibroblast growth factor bFGF in vivo are effectively solved, and bone regeneration of a critical-size bone defect part can be remarkably promoted.

The invention provides a preparation method of a recombinant cat basic fibroblast growth factor solution, and relates to the field of medicines, the preparation method comprises the following steps: constructing a cat basic fibroblast growth factor expression vector pET30a-Fe-bFGF, successfully transforming into genetic engineering escherichia coli BL21 (DE3), and screening to obtain a soluble expression Fe-bFGF strain; a recombinant escherichia coli strain BL21 (Fe-bFGF) for expressing the cat basic fibroblast growth factor is successfully constructed, and a cation and affinity chromatography purification mode is established according to protein properties, so that cat-derived recombinant basic fibroblast growth factor protein with biological activity can be purified. The cat-derived basic fibroblast growth factor for promoting the healing of the cat wound in a targeted manner has a promoting effect on the healing of the wound, so that the technical blank of the cat-derived basic fibroblast growth factor for promoting the healing of the cat wound is solved.

The invention provides a culture medium and a culture method of fibroblasts related to enameloblastoma. The culture medium comprises DMEM (Dulbecco's Modified Eagle Medium) high glucose and the following components based on the volume of the DMEM high glucose: 5v%-15v% of fetal calf serum; 2.0 to 10.0 ng / ml of a basic fibroblast growth factor; a 1% penicillin-streptomycin solution; and 1.0-5.0 [mu] g / ml of fibroblast growth factor 10. According to the culture mode of combining a culture medium formula and a digestion method with a tissue block method, the growth of fibroblasts can be promoted, the excessive proliferation of tumor cells can be inhibited, and pollution can be effectively reduced, so that the culture success rate and the cell viability of the fibroblasts related to the glamoblastoma are improved.

The application provides a prostate cancer organoid special culture medium and a culture method thereof, and belongs to the technical field of biological medicine. The prostate cancer organoid special culture medium is composed of a basic culture medium and a culture additive. The basic culture medium does not contain exogenous added Noggin recombinant protein and R-spondin recombinant protein. The culture additive comprises basic fibroblast growth factor, keratinocyte growth factor, fibroblast growth factor 10, insulin, p38MAPK signal pathway inhibitor, insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, forskolin and androgen. The prostate cancer organoid special culture medium can reduce the cost and improve the culture success rate.

The invention relates to a method for preparing iPSC and differentiating the iPSC into iNKT cells. The method comprises the following steps: (1) introducing a reprogramming induction factor into the iNKT cells, and then reprogramming; (2) culturing the reprogrammed cells to obtain iPSCs, in which the culturing process comprises: (a) culturing the reprogrammed cells in a culture medium comprising basic fibroblast growth factor (bFGF), and (b) culturing the reprogrammed cells in a culture medium comprising an antioxidant, a glycogen synthase kinase 3 (GSK-3) inhibitor, a ROCK inhibitor, and a histone deacetylase (HDAC) inhibitor; and (3) differentiating the iPSCs into the iNKT cells.

The invention discloses levobupivacaine hydrochloride-bFGF composite gel for postoperative wound analgesia and repair as well as a preparation method and application of the levobupivacaine hydrochloride-bFGF composite gel, and relates to the technical field of medicines. The levobupivacaine hydrochloride-bFGF composite gel disclosed by the invention comprises a levobupivacaine hydrochloride solid dispersion, the gel matrix is loaded with basic fibroblast growth factors; the levobupivacaine hydrochloride solid dispersion is dispersed in the gel matrix. The preparation structure constructed step by step and physically isolated is adopted, and the technical bottleneck that levobupivacaine hydrochloride and bFGF are difficult to stably compound is overcome; the structure realizes long-acting stable coexistence of the two components in a single preparation on the premise of ensuring that the biological activity of the bFGF is completely retained; the in-vitro research proves that the time sequence synergistic release of the two active substances can be accurately controlled, the animal in-vivo experiment proves that the analgesia and repair promotion effects of the composition are obviously superior to those of a single component and a simple mixture, and an innovative solution is provided for the integrated treatment of postoperative analgesia and tissue repair.

The invention provides a piezoelectric responsive composite stent based on body surface micro-motion driving and application thereof, and belongs to the technical field of biomedical materials. Comprising a piezoelectric material matrix selected from a polylactic acid-polyvinylidene fluoride composite fiber membrane with improved wet stability; the surface bioactive modification layer comprises collagen, hyaluronic acid and / or polypeptide containing an RGD sequence and is used for enhancing cell adhesion and proliferation; and the growth factor slow-release unit is composed of degradable polymer microspheres entrapped with vascular endothelial growth factors and / or basic fibroblast growth factors, and is embedded in the piezoelectric material matrix. An electric field adaptive to skin repair is generated by responding to natural micro-motion of a body surface and is used for regeneration and repair of chronic wounds, burns and full-thickness skin defects.

The invention relates to a heart organoid cryopreservation solution and application thereof, and belongs to the technical field of biology. The freezing medium capable of maintaining the structure and function stability of the heart organoid is obtained through screening, and the freezing medium is selected from one or more of fetal calf serum, dimethyl sulfoxide, a ROCK inhibitor, methylcellulose, a basic fibroblast growth factor and L-ascorbic acid. The method can effectively protect cells of the heart organoid from being damaged in the cryopreservation process, obviously reduce apoptosis of the cells, maintain the stability of the structure and function of the cryopreserved heart organoid, and provide possibility for large-scale preservation and transportation of heart organoid samples and subsequent use of a large number of heart organoid as experimental models.

The invention relates to an anti-aging composition containing umbilical cord stem cell extract and a preparation method of the anti-aging composition. The composition consists of an umbilical cord stem cell extract, a natural antioxidant, a stabilizer, a skin penetration enhancer and deionized water or a biocompatible solvent, and placenta polypeptide, a basic fibroblast growth factor (bFGF) and an epidermal growth factor (EGF) can also be added as synergistic components. The preparation method comprises the following steps: mixing the components under the protection of inert gas, homogenizing, defoaming, filling, sterilizing and the like. The composition provided by the invention has the advantages of remarkable anti-aging effect, high stability, good safety, high bioavailability and various dosage forms, and can meet different use requirements.